Miyakogusa Predicted Gene

Lj4g3v1388820.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v1388820.1 tr|I1MUM7|I1MUM7_SOYBN Malate synthase OS=Glycine
max GN=Gma.31590 PE=3 SV=1,89.28,0,MALATE_SYNTHASE,Malate synthase,
conserved site; Malate_synthase,Malate synthase; no
description,NUL,CUFF.49122.1
         (631 letters)

Database: Glyma1.pep 
           75,778 sequences; 25,431,882 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Glyma17g13730.1                                                      1072   0.0  
Glyma05g03100.1                                                      1018   0.0  
Glyma17g13740.1                                                       996   0.0  
Glyma05g03090.1                                                       454   e-127
Glyma19g25300.1                                                       110   3e-24
Glyma14g12590.1                                                       107   3e-23
Glyma03g17880.1                                                        66   1e-10

>Glyma17g13730.1 
          Length = 567

 Score = 1072 bits (2773), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 508/569 (89%), Positives = 540/569 (94%), Gaps = 3/569 (0%)

Query: 64  MDLG-YGYPPQAVAGKKIDSSYDVPEGVDIRGRYDAEFAKILTKDALKFVADLQREFRNH 122
           MD+G YGYP  AV  KKI+S YDVPEGVDIRGRYDAEFAKILTKDALKFVADLQREFRNH
Sbjct: 1   MDIGTYGYPTPAV--KKIESYYDVPEGVDIRGRYDAEFAKILTKDALKFVADLQREFRNH 58

Query: 123 IKYALECRKEAKRRYNEGAVPGFDPATRCIREGEWLCAPVPAAVCDRKVEITGPVERKMV 182
           IKYALECR+EAK++YNEGA+P FDPATR IRE EW+CAPVP AV DRKVEITGPV+RKMV
Sbjct: 59  IKYALECRREAKKKYNEGALPEFDPATRYIREQEWVCAPVPPAVADRKVEITGPVDRKMV 118

Query: 183 INALNSGAKVFMADFEDALSPSWENLMRGQVNLKDAVSGTISFHDKNRNRVYKLNDQIAK 242
           INALNSGAKVFMADFEDALSP WENLMRGQVNLKDAV+GTIS HDK RNRVYKLNDQ AK
Sbjct: 119 INALNSGAKVFMADFEDALSPGWENLMRGQVNLKDAVAGTISLHDKARNRVYKLNDQTAK 178

Query: 243 LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHNHSTFRRTQGAGFGPFFYLPKMEHS 302
           LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYH++S FRRTQGAGFGPFFYLPKMEHS
Sbjct: 179 LFVRPRGWHLPEAHILIDGEPATGCLVDFGLYFYHSYSAFRRTQGAGFGPFFYLPKMEHS 238

Query: 303 REAKIWNNVFERAEKMARIERGSIRATVLIETLPAVFQMDEILYELREHSVGLNCGRWDY 362
           REAKIWNNVFE+AEK+A IERGSIRATVLIETLPAVFQM+EILYEL++HSVGLNCGRWDY
Sbjct: 239 REAKIWNNVFEKAEKVAGIERGSIRATVLIETLPAVFQMNEILYELKDHSVGLNCGRWDY 298

Query: 363 IFSYVKTFQAHPDRLLPDRVLVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDD 422
           IFSYVKTFQAHPDRLLPDRVLVGMTQHFM+SYSDLLIRTCHRRGVHAMGGMAAQIPI++D
Sbjct: 299 IFSYVKTFQAHPDRLLPDRVLVGMTQHFMKSYSDLLIRTCHRRGVHAMGGMAAQIPIKED 358

Query: 423 PAANEAAVELVRKDKLREVKAGHDGTWAAHPGLIPTCMEVFNSNMGTAPNQIKTMKRDDA 482
           P ANE A+ELVRKDKLREVKAGHDGTWAAHPGLIP CME+FN+NMG A NQI T+KR+D 
Sbjct: 359 PVANEVALELVRKDKLREVKAGHDGTWAAHPGLIPACMEIFNNNMGNASNQIDTVKREDG 418

Query: 483 ASITEEDLLQIPRGARTMDGLRLNTRVGIQYVAAWLTGAGSVPLYNLMEDAATAEISRVQ 542
           A+ITE+DLLQIPRGARTM+GLRLNTRVGIQYVAAWLTG+GSVPLYNLMEDAATAEISRVQ
Sbjct: 419 ANITEQDLLQIPRGARTMEGLRLNTRVGIQYVAAWLTGSGSVPLYNLMEDAATAEISRVQ 478

Query: 543 NWQWIKYGVELNGDGVGVKVNKELFGRVVEEEMARIEREVGKEKFKKGMYKEACKIFTRQ 602
           NWQW+KYGVELNGDG+GVKVNKELFGRVVEEEMARIE+EVG EKFKKGMYKEACKIFTRQ
Sbjct: 479 NWQWLKYGVELNGDGLGVKVNKELFGRVVEEEMARIEKEVGTEKFKKGMYKEACKIFTRQ 538

Query: 603 CTSLALDDFLTLDAYNSIVMHHPREQSKL 631
           CTS  LDDFLTLDAYN IV+HHPRE SKL
Sbjct: 539 CTSPMLDDFLTLDAYNYIVVHHPRETSKL 567


>Glyma05g03100.1 
          Length = 556

 Score = 1018 bits (2631), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 475/545 (87%), Positives = 513/545 (94%)

Query: 83  SYDVPEGVDIRGRYDAEFAKILTKDALKFVADLQREFRNHIKYALECRKEAKRRYNEGAV 142
           +Y+VPEGVDI GRYDAEFAKILTKDALKFVA+LQREFR+ IK+ALE R+EAK++YNEG +
Sbjct: 5   TYEVPEGVDILGRYDAEFAKILTKDALKFVAELQREFRSDIKHALESRREAKKKYNEGTL 64

Query: 143 PGFDPATRCIREGEWLCAPVPAAVCDRKVEITGPVERKMVINALNSGAKVFMADFEDALS 202
           PGFDPAT  IR+ EWLCAPVP A  DRKVEITGPV+RKMVINALNS AKVFMAD EDALS
Sbjct: 65  PGFDPATSHIRDQEWLCAPVPPAAADRKVEITGPVDRKMVINALNSAAKVFMADLEDALS 124

Query: 203 PSWENLMRGQVNLKDAVSGTISFHDKNRNRVYKLNDQIAKLFVRPRGWHLPEAHILIDGE 262
           P+WENL+ GQVNLKDAV GTISFHDK RNRVYKLNDQ AKLFVRPRGWHLPEAHILIDGE
Sbjct: 125 PTWENLISGQVNLKDAVDGTISFHDKVRNRVYKLNDQTAKLFVRPRGWHLPEAHILIDGE 184

Query: 263 PATGCLVDFGLYFYHNHSTFRRTQGAGFGPFFYLPKMEHSREAKIWNNVFERAEKMARIE 322
           PATGCLVDFGLYFYHNHS FRRTQGAGFGPFFYLPKMEHSREAKIWN+VFE+AE +A IE
Sbjct: 185 PATGCLVDFGLYFYHNHSAFRRTQGAGFGPFFYLPKMEHSREAKIWNSVFEKAEDVAGIE 244

Query: 323 RGSIRATVLIETLPAVFQMDEILYELREHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRV 382
           RGSIRATVLIETLPAVFQMDEILYEL+EHSVGLNCGRWDYIFSYVKTFQ HPDRLLPDRV
Sbjct: 245 RGSIRATVLIETLPAVFQMDEILYELKEHSVGLNCGRWDYIFSYVKTFQTHPDRLLPDRV 304

Query: 383 LVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPAANEAAVELVRKDKLREVK 442
            VGMTQHFM+SYSDLLIRTCHRRGVHAMGGMAAQIPI+DDP ANEAA+ELVRKDKLREVK
Sbjct: 305 QVGMTQHFMKSYSDLLIRTCHRRGVHAMGGMAAQIPIKDDPVANEAALELVRKDKLREVK 364

Query: 443 AGHDGTWAAHPGLIPTCMEVFNSNMGTAPNQIKTMKRDDAASITEEDLLQIPRGARTMDG 502
           AGHDGTWAAHPGLIP+CMEVFN+NMG APNQI T+KRDDAA++TE+DLLQIP G RTM+G
Sbjct: 365 AGHDGTWAAHPGLIPSCMEVFNNNMGNAPNQIDTVKRDDAANLTEQDLLQIPTGTRTMEG 424

Query: 503 LRLNTRVGIQYVAAWLTGAGSVPLYNLMEDAATAEISRVQNWQWIKYGVELNGDGVGVKV 562
           LRLNTRVGIQY+AAWL+G+G VPLYNLMEDAATAEISRVQNWQW+KYGVEL+GDG+G KV
Sbjct: 425 LRLNTRVGIQYLAAWLSGSGCVPLYNLMEDAATAEISRVQNWQWLKYGVELDGDGLGEKV 484

Query: 563 NKELFGRVVEEEMARIEREVGKEKFKKGMYKEACKIFTRQCTSLALDDFLTLDAYNSIVM 622
           NK+LF RVVEEEMARIE+EVG EKF+KGMYKEACKIFTRQCTSL LDDFLTLDAYN IV+
Sbjct: 485 NKDLFHRVVEEEMARIEKEVGTEKFEKGMYKEACKIFTRQCTSLTLDDFLTLDAYNYIVV 544

Query: 623 HHPRE 627
           HHP+E
Sbjct: 545 HHPKE 549


>Glyma17g13740.1 
          Length = 556

 Score =  996 bits (2576), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 466/545 (85%), Positives = 507/545 (93%)

Query: 83  SYDVPEGVDIRGRYDAEFAKILTKDALKFVADLQREFRNHIKYALECRKEAKRRYNEGAV 142
           +YDVPEGVDI GRYDAEFAKIL KDALKFVA+LQREFRN IK+ALE R+EAK++YNEG++
Sbjct: 5   TYDVPEGVDILGRYDAEFAKILAKDALKFVAELQREFRNDIKHALERRREAKKKYNEGSL 64

Query: 143 PGFDPATRCIREGEWLCAPVPAAVCDRKVEITGPVERKMVINALNSGAKVFMADFEDALS 202
           PGFDPAT  IRE EW+CAPVP A  DRKVEITGPV+RKMVINALNS AKVFMAD EDALS
Sbjct: 65  PGFDPATSDIREQEWVCAPVPPAAADRKVEITGPVDRKMVINALNSAAKVFMADLEDALS 124

Query: 203 PSWENLMRGQVNLKDAVSGTISFHDKNRNRVYKLNDQIAKLFVRPRGWHLPEAHILIDGE 262
           P+WENL+ GQVNLKDAV GTISFHDK RNR+YKLNDQ AKLFVRPRGWHLPEAHILIDGE
Sbjct: 125 PTWENLISGQVNLKDAVDGTISFHDKVRNRIYKLNDQTAKLFVRPRGWHLPEAHILIDGE 184

Query: 263 PATGCLVDFGLYFYHNHSTFRRTQGAGFGPFFYLPKMEHSREAKIWNNVFERAEKMARIE 322
           PATGCLVDFGLYFYHNHS FRRTQGAGFGPFFYLPKMEHSREAKIWN+VFE+AE +  I+
Sbjct: 185 PATGCLVDFGLYFYHNHSAFRRTQGAGFGPFFYLPKMEHSREAKIWNSVFEKAEDVVGIK 244

Query: 323 RGSIRATVLIETLPAVFQMDEILYELREHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRV 382
           RGSIRATVLIETLPAVFQM+EILYEL+EHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRV
Sbjct: 245 RGSIRATVLIETLPAVFQMNEILYELKEHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRV 304

Query: 383 LVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPAANEAAVELVRKDKLREVK 442
            VGMTQHFM+SYSDLLIRTCHRRGVHAMGGMAAQIPI+DDP ANEAA+ELVRKDKLREVK
Sbjct: 305 QVGMTQHFMKSYSDLLIRTCHRRGVHAMGGMAAQIPIKDDPVANEAALELVRKDKLREVK 364

Query: 443 AGHDGTWAAHPGLIPTCMEVFNSNMGTAPNQIKTMKRDDAASITEEDLLQIPRGARTMDG 502
           AGHDGTWAAHPGLIP CME+FN+NMG A NQI T+KR+DAA++TE+DLLQIP G RTM+G
Sbjct: 365 AGHDGTWAAHPGLIPACMEIFNNNMGNASNQIDTVKREDAANLTEQDLLQIPIGTRTMEG 424

Query: 503 LRLNTRVGIQYVAAWLTGAGSVPLYNLMEDAATAEISRVQNWQWIKYGVELNGDGVGVKV 562
           LRLNTRVGIQY+AAWL+G+G VPLYNLMEDAATAEISRVQNWQW+KYGVEL+ DG+G KV
Sbjct: 425 LRLNTRVGIQYLAAWLSGSGCVPLYNLMEDAATAEISRVQNWQWLKYGVELDEDGLGEKV 484

Query: 563 NKELFGRVVEEEMARIEREVGKEKFKKGMYKEACKIFTRQCTSLALDDFLTLDAYNSIVM 622
           NK+LF RVVEEEMARIE+EVG EKF+KGMYK ACKIFTRQCTS  LDDFLTLDAYN IV+
Sbjct: 485 NKDLFDRVVEEEMARIEKEVGTEKFEKGMYKAACKIFTRQCTSPTLDDFLTLDAYNYIVV 544

Query: 623 HHPRE 627
           HH +E
Sbjct: 545 HHAKE 549


>Glyma05g03090.1 
          Length = 397

 Score =  454 bits (1168), Expect = e-127,   Method: Compositional matrix adjust.
 Identities = 275/562 (48%), Positives = 310/562 (55%), Gaps = 184/562 (32%)

Query: 89  GVDIRGRYDAEFAKILTKDALKFVADLQREFRNHIKYALECRKEAKRRYNEGAVPGFDPA 148
           GVDIRGRY+AEFAKILTKDALKFVA+LQR+         E R+E KRRYNEGA+PGFDPA
Sbjct: 1   GVDIRGRYNAEFAKILTKDALKFVAELQRD--------AEERRETKRRYNEGALPGFDPA 52

Query: 149 TRCIREGEWLCAPVPAAVCDRKVEITGPVERKMVINALNSGAKVFMADFEDALSPSWENL 208
           TR IRE EW+CAPVP A  DR            +    N+ +         ALSPSW+N 
Sbjct: 53  TRYIREQEWVCAPVPPAGADRNT---------YLFRNKNTESCY-------ALSPSWKNP 96

Query: 209 MRGQVNLKDAVSGTISFHDKNRNRVYKLNDQIAKLFVRPRGWHLPEAHILIDGEPATGCL 268
           MRGQV LKDAV+GTIS HDK RNR YKLNDQ  KLFV+PRGW+LPEAHILIDGEPATGCL
Sbjct: 97  MRGQVKLKDAVAGTISLHDKVRNRDYKLNDQTTKLFVQPRGWYLPEAHILIDGEPATGCL 156

Query: 269 VDFGLYFYHNHSTFRRTQGAGFGPFFYLPKMEHSR-------------------EAKIWN 309
           VDFGLYFYHNH  FRRTQGA FGPFFYLPKMEHS                    E +IW 
Sbjct: 157 VDFGLYFYHNHLAFRRTQGASFGPFFYLPKMEHSSFITFNPNTQLSFTSFRLLWEVRIWF 216

Query: 310 NVFERAEKMARIERGSIRATVLIETLPAVFQMDEILYELREHSVGLNCGRWDYIFSYVKT 369
            V +       IE GSIR T LIETLP           L+EHSVGLNCGRWDYIF+Y+  
Sbjct: 217 KVLD-------IEGGSIRTTDLIETLPT----------LKEHSVGLNCGRWDYIFNYITD 259

Query: 370 FQAHPDRLLPDRVLVGMTQHFMRSYSDLLIRTCHRRGVHAMGGMAAQIPIRDDPAANEAA 429
           +           +L G    F+  + +    TC + G       +  IP           
Sbjct: 260 YN----------ILDGC---FITIFFNNCTGTC-KEGQAERSEGSGLIP----------- 294

Query: 430 VELVRKDKLREVKAGHDGTWAAHPGLIPTCMEVFNSNMGTAPNQIKTMKRDDAASITEED 489
                                       TCM+VFN+NMG APN +     +DAA      
Sbjct: 295 ----------------------------TCMKVFNNNMGNAPNLM-----EDAA------ 315

Query: 490 LLQIPRGARTMDGLRLNTRVGIQYVAAWLTGAGSVPLYNLMEDAATAEISRVQNWQWIKY 549
                                                        TAEISRVQNWQW+KY
Sbjct: 316 ---------------------------------------------TAEISRVQNWQWLKY 330

Query: 550 GVELNGDGVGVKVNKELFGRVVEEEMARIEREVGKEKFKKGMYKEACKIFTRQCTSLALD 609
                GDG+GVK+          EEM RIE+EVG  KF KGMYKEACKIFTRQCTS +LD
Sbjct: 331 -----GDGLGVKI----------EEMHRIEKEVGTYKFMKGMYKEACKIFTRQCTSPSLD 375

Query: 610 DFLTLDAYNSIVMHHPREQSKL 631
           DFLTLDAYN IV+HHPRE SKL
Sbjct: 376 DFLTLDAYNYIVLHHPRETSKL 397


>Glyma19g25300.1 
          Length = 194

 Score =  110 bits (276), Expect = 3e-24,   Method: Compositional matrix adjust.
 Identities = 49/62 (79%), Positives = 56/62 (90%)

Query: 414 AAQIPIRDDPAANEAAVELVRKDKLREVKAGHDGTWAAHPGLIPTCMEVFNSNMGTAPNQ 473
           AA+IPI++DP ANE A+ LVRKDKLREVKAG+DGTWAAHPGLIP CME+FN+NMG APNQ
Sbjct: 1   AAEIPIKEDPVANEVALGLVRKDKLREVKAGYDGTWAAHPGLIPVCMEIFNNNMGNAPNQ 60

Query: 474 IK 475
            K
Sbjct: 61  NK 62


>Glyma14g12590.1 
          Length = 115

 Score =  107 bits (268), Expect = 3e-23,   Method: Composition-based stats.
 Identities = 49/73 (67%), Positives = 62/73 (84%)

Query: 303 REAKIWNNVFERAEKMARIERGSIRATVLIETLPAVFQMDEILYELREHSVGLNCGRWDY 362
           REAKIWN VFE+ EK+  IE+GSIR TVLI+TL A+FQMD+I+YEL++  +GLN  +W+Y
Sbjct: 35  REAKIWNIVFEKNEKVVGIEKGSIRVTVLIQTLCALFQMDKIMYELQDCLMGLNRRQWNY 94

Query: 363 IFSYVKTFQAHPD 375
           IF+YVKTFQAH D
Sbjct: 95  IFNYVKTFQAHSD 107


>Glyma03g17880.1 
          Length = 126

 Score = 65.9 bits (159), Expect = 1e-10,   Method: Composition-based stats.
 Identities = 30/42 (71%), Positives = 36/42 (85%), Gaps = 3/42 (7%)

Query: 373 HPDRLLPD-RVLVGMTQHFMRSYSDLLIRTCHRRGVHAMGGM 413
           HP  +LPD RVLVG+  HFM+SY DLLI+TCHRRGVH++GGM
Sbjct: 64  HPYYVLPDDRVLVGI--HFMKSYYDLLIKTCHRRGVHSLGGM 103