Miyakogusa Predicted Gene
- Lj4g3v1000030.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj4g3v1000030.1 tr|Q7Z372|Q7Z372_HUMAN Microspherule protein 1
OS=Homo sapiens GN=DKFZp686N07218 PE=2
SV=1,37.64,2e-18,MCRS_N,Microspherule protein, N-terminal domain;
FHA,Forkhead-associated (FHA) domain; FHA_DOMAIN,Fo,CUFF.48273.1
(713 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma02g05750.1 332 1e-90
Glyma16g24410.1 303 3e-82
Glyma02g05750.2 275 2e-73
Glyma04g02970.1 197 4e-50
Glyma06g02980.1 181 2e-45
Glyma17g31620.1 88 3e-17
Glyma12g16100.1 78 4e-14
>Glyma02g05750.1
Length = 610
Score = 332 bits (850), Expect = 1e-90, Method: Compositional matrix adjust.
Identities = 207/438 (47%), Positives = 265/438 (60%), Gaps = 69/438 (15%)
Query: 298 GESHGDMDLVSDPGNEVRPSSATAAVQSPHPERSEDLRICVLNTEDTDVPVNDSVNLSVV 357
G D VS+ GN+ + ++ AVQSP PER E ICVLNTEDTD+P ND ++ ++
Sbjct: 214 GGGCADQHCVSESGND---AGSSGAVQSPLPERGEGYMICVLNTEDTDIPSNDPTDIPIL 270
Query: 358 APRSVAMKSQRVANDCXXXXXXXXXXXXXXXXXXXXXXXPRSVPLKSQSIAKEVGYSDPS 417
P +PLKSQ + +E+G + S
Sbjct: 271 --------------------------------------FPELMPLKSQPVIQELGCLESS 292
Query: 418 ISNQRKNVPAGSLEKE----------EMNRAGLVPTINSSNTPIGVGMKAEISTGNSISA 467
I+N+R+N GSL+ E + GL+P +NSS+ P+ +G+K+E NS SA
Sbjct: 293 INNKRRNESDGSLKIEAVLSQSFAALQTVWQGLMPNVNSSHRPLVLGLKSENPGRNSTSA 352
Query: 468 VSRQNNNVNIK--PSQSRL----------GHAKQEEIDALASAVVYAHPKAKDHMASKKL 515
VSRQNNNVN PS RL GH KQEE +A ASA H KA++H A K
Sbjct: 353 VSRQNNNVNANINPSHGRLVRATVMPASDGHLKQEETNAPASA----HSKAEEHTALSKS 408
Query: 516 EEKSLSHRQXXXXXXXXXXXXXXXXXIPYYSDAEKMILEMDLCPADEDINASREVLEYQH 575
E KSLS Q +P ++D E+MILEMDL PAD+D NASREVL++QH
Sbjct: 409 EVKSLSLDQEGCDIKDDDDEDNDNE-LPNFADVEEMILEMDLSPADQDTNASREVLQFQH 467
Query: 576 EETKRAIMRLEQCAHAFTQRAIASEGALAVLYGRKMKQYIKKSEVILGRATEGVQVDIDL 635
E++KR IMRLEQ A + RAI+S+GA AV+YGR +K YI KS+VILGR T V VDIDL
Sbjct: 468 EQSKRTIMRLEQGAQSSMGRAISSQGAFAVVYGRILKTYITKSKVILGRETHDVHVDIDL 527
Query: 636 GREGR-ANKISRRQALIKMDANGSFIIKNLGRRSIFLNGKEVAHGQLRGLSPSCLIEITG 694
GREG+ A +ISRRQA+IK++A+GSFII NLG+RSIFLNGKE+A GQ RGLS LIEI G
Sbjct: 528 GREGQEATRISRRQAVIKLEADGSFIIINLGKRSIFLNGKEIATGQARGLSAGSLIEILG 587
Query: 695 LSFVFEFNSMSVKRFLEN 712
+S +FE N+ V++FLEN
Sbjct: 588 ISLIFETNNGCVRKFLEN 605
Score = 93.6 bits (231), Expect = 6e-19, Method: Compositional matrix adjust.
Identities = 51/111 (45%), Positives = 72/111 (64%), Gaps = 14/111 (12%)
Query: 10 WIPEDDFLLKNAVEAGASLESLAKGAVRFSRRYSLTQIQDRWRSILYGPEESAKAAASML 69
W+P+DDFLLKNA+E GASLESLAKGAVRFSRR+S+T+++DRW+++LY P+ SA A A+M
Sbjct: 4 WLPQDDFLLKNAIEGGASLESLAKGAVRFSRRFSVTELRDRWQALLYDPDVSAAALAAMA 63
Query: 70 NLELSRFXXXXXXXXXXXXXXXNGRRVKMKIETFRKRKRDSLRKQYYERMR 120
+LE +++ +KRK +S+RK Y+ R
Sbjct: 64 HLEAAKYGGAAG--------------TSEGGGGGKKRKSESIRKHYFAMQR 100
>Glyma16g24410.1
Length = 576
Score = 303 bits (777), Expect = 3e-82, Method: Compositional matrix adjust.
Identities = 216/523 (41%), Positives = 286/523 (54%), Gaps = 89/523 (17%)
Query: 205 GNAVLSVPGGAAVLGEEGVRISDSLMDLTNGDDEGLLLDGTAKEKLGCD-DKIVSENVDA 263
GN VL + AVL ++ L +L N +DE + +D KE L D DK +NVD+
Sbjct: 123 GNGVLRLNAPNAVLRDDAKNDLKCLFNLAN-EDEVVFMDLVRKEVLAADKDKPSYDNVDS 181
Query: 264 SVVVPELLKLDTGTKLAVRRGSSAAE-LGVGFNSSGESHGDMDLVSDPGNEVRPSSATAA 322
++ + V+ E LG G D VS+ GN + ++ A
Sbjct: 182 LLL---------SSPCDVQGDDDGREPLGGGC-------ADQHCVSESGNN---AGSSGA 222
Query: 323 VQSPHPERSEDLRICVLNTEDTDVPVNDSVNLSVVAPRSVAMKSQRVANDCXXXXXXXXX 382
VQ+PH E+SE ICVLNTEDTD+P ND+ ++ ++ P + +
Sbjct: 223 VQTPHAEQSEGFMICVLNTEDTDIPSNDTTDIPILVPENES------------------- 263
Query: 383 XXXXXXXXXXXXXXPRSVPLKSQSIAKEVGYSDPSISNQRKNVPAGSLEKEEMNRAGLVP 442
S+ KEV S S + Q R GLVP
Sbjct: 264 ---------------------DGSLEKEVVLSQSSAALQ-------------TVRQGLVP 289
Query: 443 TINSSNTPIGVGMKAEISTGNSISAVSRQNNNVNIKPS--QSRL----------GHAKQE 490
+NSS+ P+ +G+K+E NSISAVSRQNNNVN+ + SRL GH KQE
Sbjct: 290 NVNSSHRPLVLGLKSENPGRNSISAVSRQNNNVNVNINSRHSRLVRATVMPASDGHLKQE 349
Query: 491 EIDALASAVVYAHPKAKDHMASKKLEEKSLSHRQXXXXXXXXXXXXXXXXXIPYYSDAEK 550
+A H KA++H A K E K LS +P +SD E+
Sbjct: 350 VECVETNAPSSVHAKAEEHKALSKSEAKPLS-LDHEGGDIEDGDDNDNDDELPNFSDVEE 408
Query: 551 MILEMDLCPADEDINASREVLEYQHEETKRAIMRLEQCAHAFTQRAIASEGALAVLYGRK 610
MILEMDL P D++ NASREVL YQ +E+KR IMRLEQ A + RAI+S+GA AV+YGR
Sbjct: 409 MILEMDLSPMDQETNASREVLRYQLDESKRTIMRLEQSAQSSMGRAISSQGAFAVVYGRI 468
Query: 611 MKQYIKKSEVILGRATEGVQVDIDLGREGR-ANKISRRQALIKMDANGSFIIKNLGRRSI 669
+++YIK S+VILGRAT V VDIDLG+EG+ A +ISRRQA+IK++A+GSFIIKNLG+R I
Sbjct: 469 LEKYIKTSKVILGRATNDVHVDIDLGKEGQVATRISRRQAVIKLEADGSFIIKNLGKRCI 528
Query: 670 FLNGKEVAHGQLRGLSPSCLIEITGLSFVFEFNSMSVKRFLEN 712
FLNGKE+A GQ+RGLS LIEI G+ +FE N+ V++FLEN
Sbjct: 529 FLNGKEIATGQVRGLSAGSLIEILGIPLIFETNNSCVRKFLEN 571
Score = 99.0 bits (245), Expect = 2e-20, Method: Compositional matrix adjust.
Identities = 67/188 (35%), Positives = 99/188 (52%), Gaps = 9/188 (4%)
Query: 10 WIPEDDFLLKNAVEAGASLESLAKGAVRFSRRYSLTQIQDRWRSILYGPEESAKAAASML 69
W+P+DDFLLKNA+E GASLESLAKGAVRFSRR+S+T+++DRW+++LY P+ SA A A+M
Sbjct: 4 WLPQDDFLLKNAIEGGASLESLAKGAVRFSRRFSVTELRDRWQALLYDPDVSAAARAAMA 63
Query: 70 NLELSRFXXXXXXXXXXXXXXXNGRRVKMKIETFRKRKRDSLRKQYYERMRMRKLIRMEV 129
NLEL+++ N ++ +KR R R R K +E
Sbjct: 64 NLELTKYGGGTGTGEGGGGKKRNSESIRKHYSAMQKRLR---------RCRHGKSHGLEG 114
Query: 130 LDSLDIGFDDGVRIVDDAVGYEGDEIGGNLNCDMNHLHVDSVVGFDEAGVGPLNSMSDAP 189
+L +GV ++ D+ +L C N + D VV D L + D P
Sbjct: 115 RVNLKGECGNGVLRLNAPNAVLRDDAKNDLKCLFNLANEDEVVFMDLVRKEVLAADKDKP 174
Query: 190 LWKTIEDV 197
+ ++ +
Sbjct: 175 SYDNVDSL 182
>Glyma02g05750.2
Length = 285
Score = 275 bits (702), Expect = 2e-73, Method: Compositional matrix adjust.
Identities = 160/285 (56%), Positives = 198/285 (69%), Gaps = 18/285 (6%)
Query: 441 VPTINSSNTPIGVGMKAEISTGNSISAVSRQNNNVNIK--PSQSRL----------GHAK 488
+P +NSS+ P+ +G+K+E NS SAVSRQNNNVN PS RL GH K
Sbjct: 1 MPNVNSSHRPLVLGLKSENPGRNSTSAVSRQNNNVNANINPSHGRLVRATVMPASDGHLK 60
Query: 489 QEEIDALASAVVYAHPKAKDHMASKKLEEKSLSHRQXXXXXXXXXXXXXXXXXIPYYSDA 548
QEE +A ASA H KA++H A K E KSLS Q +P ++D
Sbjct: 61 QEETNAPASA----HSKAEEHTALSKSEVKSLSLDQEGCDIKDDDDEDNDNE-LPNFADV 115
Query: 549 EKMILEMDLCPADEDINASREVLEYQHEETKRAIMRLEQCAHAFTQRAIASEGALAVLYG 608
E+MILEMDL PAD+D NASREVL++QHE++KR IMRLEQ A + RAI+S+GA AV+YG
Sbjct: 116 EEMILEMDLSPADQDTNASREVLQFQHEQSKRTIMRLEQGAQSSMGRAISSQGAFAVVYG 175
Query: 609 RKMKQYIKKSEVILGRATEGVQVDIDLGREGR-ANKISRRQALIKMDANGSFIIKNLGRR 667
R +K YI KS+VILGR T V VDIDLGREG+ A +ISRRQA+IK++A+GSFII NLG+R
Sbjct: 176 RILKTYITKSKVILGRETHDVHVDIDLGREGQEATRISRRQAVIKLEADGSFIIINLGKR 235
Query: 668 SIFLNGKEVAHGQLRGLSPSCLIEITGLSFVFEFNSMSVKRFLEN 712
SIFLNGKE+A GQ RGLS LIEI G+S +FE N+ V++FLEN
Sbjct: 236 SIFLNGKEIATGQARGLSAGSLIEILGISLIFETNNGCVRKFLEN 280
>Glyma04g02970.1
Length = 748
Score = 197 bits (501), Expect = 4e-50, Method: Compositional matrix adjust.
Identities = 96/173 (55%), Positives = 122/173 (70%), Gaps = 1/173 (0%)
Query: 542 IPYYSDAEKMILEMDLCPAD-EDINASREVLEYQHEETKRAIMRLEQCAHAFTQRAIASE 600
+P YSD E M+L+MDL P D +D + EV YQH E+KRAIMRLEQ +H+ QRAI S
Sbjct: 569 LPSYSDVEAMVLDMDLDPHDHQDSYYNEEVSRYQHVESKRAIMRLEQGSHSCIQRAIDSH 628
Query: 601 GALAVLYGRKMKQYIKKSEVILGRATEGVQVDIDLGREGRANKISRRQALIKMDANGSFI 660
GA A+LY R K YIKK EV+LGRATE V VDIDLG+ G N ISRRQA+IKM +G+F
Sbjct: 629 GAFAILYSRHSKHYIKKPEVLLGRATESVPVDIDLGKGGHGNAISRRQAIIKMAKDGTFY 688
Query: 661 IKNLGRRSIFLNGKEVAHGQLRGLSPSCLIEITGLSFVFEFNSMSVKRFLENV 713
IKN G+ SI +N KEV GQ + L +CL+E+ G+ +FE N VK++L+ +
Sbjct: 689 IKNFGKSSILVNSKEVHTGQSQRLHSNCLVEVRGMPLIFEINQSRVKQYLDYI 741
Score = 115 bits (289), Expect = 1e-25, Method: Compositional matrix adjust.
Identities = 67/128 (52%), Positives = 80/128 (62%), Gaps = 11/128 (8%)
Query: 10 WIPEDDFLLKNAVEAGASLESLAKGAVRFSRRYSLTQIQDRWRSILYGPEESAKAAASML 69
WIPEDD LLKNAVEAGASLESLAKGAV+FSR+YSL +IQDRW S+LY P SA+AAA M
Sbjct: 10 WIPEDDLLLKNAVEAGASLESLAKGAVQFSRKYSLKEIQDRWYSLLYDPVISAEAAAGMT 69
Query: 70 NLELSRFXXXXXXXXXXXXXXXNGRRVKMKIETFRKRKRDSLRKQYYERMRMRKLIRMEV 129
N ELS G + K+ + RK +S+R YY R K IR +
Sbjct: 70 NFELS-------ASPLPSKFYRFGHLKERKVVS-ATRKSESVRNLYYARC---KRIRNSM 118
Query: 130 LDSLDIGF 137
L S+D+ F
Sbjct: 119 LTSMDLSF 126
>Glyma06g02980.1
Length = 720
Score = 181 bits (459), Expect = 2e-45, Method: Compositional matrix adjust.
Identities = 92/173 (53%), Positives = 116/173 (67%), Gaps = 1/173 (0%)
Query: 542 IPYYSDAE-KMILEMDLCPADEDINASREVLEYQHEETKRAIMRLEQCAHAFTQRAIASE 600
+P YSD E ++ +D + EV YQH E+KRAIMRLE AH+ QRAI S
Sbjct: 541 LPSYSDVEAMLLDMDLDPDDHQDSYYNEEVSRYQHVESKRAIMRLELGAHSCIQRAIDSH 600
Query: 601 GALAVLYGRKMKQYIKKSEVILGRATEGVQVDIDLGREGRANKISRRQALIKMDANGSFI 660
GA A+LY R K YIKK EV+LGRATEGV VDIDLG+ G N ISRRQA+IKMD +GSF
Sbjct: 601 GAFAILYSRHSKHYIKKPEVLLGRATEGVPVDIDLGKGGHGNAISRRQAIIKMDKDGSFY 660
Query: 661 IKNLGRRSIFLNGKEVAHGQLRGLSPSCLIEITGLSFVFEFNSMSVKRFLENV 713
IKNLG+ I +N KEV GQ + L CLIE+ G+ +FE N VK++++++
Sbjct: 661 IKNLGKSPILVNNKEVHTGQSQRLHSDCLIEVRGMPLIFEINQSRVKQYVDHI 713
Score = 134 bits (336), Expect = 5e-31, Method: Compositional matrix adjust.
Identities = 128/386 (33%), Positives = 179/386 (46%), Gaps = 57/386 (14%)
Query: 10 WIPEDDFLLKNAVEAGASLESLAKGAVRFSRRYSLTQIQDRWRSILYGPEESAKAAASML 69
WI EDD LLKN+VEAGASLESLAKGAV+FSR+YS+ +IQDRW S+LY P SA+AAA+M
Sbjct: 10 WISEDDLLLKNSVEAGASLESLAKGAVQFSRKYSIKEIQDRWYSLLYDPVISAEAAANMT 69
Query: 70 NLELSRFXXXXXXXXXXXXXXXNGRRVKMKIETFRKRKRDSLRKQYYERMRMRKLIRMEV 129
NLELS G + K+ + RK DS+R YY R RK IR +
Sbjct: 70 NLELSALPLPSKFYRF-------GHSKERKVVS-ATRKSDSVRNLYYAR---RKRIRNSM 118
Query: 130 LDSLDIGFDDGVRIVDDAVG----YEGDEIGGNLNCDMNHLHVDSVVGFDEAGVG-PLNS 184
L S+D+ F +VD G + D + G NC + D + P N
Sbjct: 119 LTSMDLSF-----LVDSENGNYAAHGSDPLSG--NCMPEGGTSNHFSSLDPSQYAFPENV 171
Query: 185 MSD-------APLWKTIEDVSAPVLPSGNAVLSVPGGAAVLGEEGVRISDSLMDLTNGDD 237
M D A +W T E + LP + G A L E +S+SL+ ++ ++
Sbjct: 172 MDDNVASDRVAAVWGTDESIQEHDLPCDGFSDPIACGDAYLEE----LSNSLLHFSS-EE 226
Query: 238 EGLLLD-----GTAKEKLGCDDKIVSENVDASVVVPELLKLDTGTKLAVRRG-------S 285
E +L+D G K G ++ +N V ++ + D L + S
Sbjct: 227 EQILMDVDGEEGIEKSYFGGLTSLL-QNSTNDVNSDQISEKDETESLMASQAHVINQSVS 285
Query: 286 SAAEL--GVGFNSSGESHGDMDLVSDPGNEVRPSSATAAVQSPHPERSEDLRICVLNTED 343
EL +G +SSG L P E + SS+ AA P+ + C +NTE
Sbjct: 286 CHKELDDNLGSSSSG-------LQVVPKLEFQMSSSVAATDPQFPDLINESMSCSINTEH 338
Query: 344 TDVPVNDSVNLSVVAPRSVAMKSQRV 369
++P ND V L P + S ++
Sbjct: 339 QEIPENDDVFLPFDVPPVIFPPSSKL 364
>Glyma17g31620.1
Length = 135
Score = 88.2 bits (217), Expect = 3e-17, Method: Composition-based stats.
Identities = 47/86 (54%), Positives = 56/86 (65%), Gaps = 2/86 (2%)
Query: 564 INASREVLEYQHEETKRAIMRLEQCAHAFTQRAIASEGALAVLYGRKMKQYIKKSEVILG 623
+N + Y H +KRA MRLEQ +H+ +RAI S GA A+LY R K YIKK EV+LG
Sbjct: 26 LNGKGACISYYHC-SKRATMRLEQGSHSCIERAIDSHGAFAILYSRHSKHYIKKPEVLLG 84
Query: 624 RATEGVQVDIDLG-REGRANKISRRQ 648
RAT+ V VDIDLG G N ISRR
Sbjct: 85 RATQSVPVDIDLGINGGHVNAISRRH 110
>Glyma12g16100.1
Length = 132
Score = 77.8 bits (190), Expect = 4e-14, Method: Composition-based stats.
Identities = 39/67 (58%), Positives = 46/67 (68%)
Query: 570 VLEYQHEETKRAIMRLEQCAHAFTQRAIASEGALAVLYGRKMKQYIKKSEVILGRATEGV 629
V YQH E+ RAIMRLEQ +++ QRAI S G ++LY YIKK EV+LG ATE V
Sbjct: 66 VSRYQHVESMRAIMRLEQGSYSCIQRAIDSHGPFSILYSCHSNHYIKKPEVLLGWATESV 125
Query: 630 QVDIDLG 636
VDIDLG
Sbjct: 126 PVDIDLG 132