Miyakogusa Predicted Gene

Lj3g3v2888300.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v2888300.1 Non Characterized Hit- tr|I3SNX6|I3SNX6_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,100,0,EF_HAND_2,EF-HAND 2; EF-hand, calcium binding
motif,Calcium-binding EF-hand; EF-hand,NULL; EF_hand_6,CUFF.44880.1
         (124 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g052885.1 | calcium-binding EF-hand protein | HC | chr1:21...   187   3e-48
Medtr2g089210.1 | calcium-binding EF-hand protein | HC | chr2:37...   186   3e-48
Medtr2g081580.1 | calcium-binding EF-hand protein | HC | chr2:34...   157   2e-39
Medtr8g466250.1 | calcium-binding EF-hand protein | HC | chr8:23...   150   2e-37
Medtr2g011400.1 | calcium-binding EF-hand protein | HC | chr2:27...   139   8e-34
Medtr8g027180.1 | calcium-binding EF-hand protein | HC | chr8:95...   124   3e-29
Medtr2g081300.1 | calcium-binding EF-hand protein | HC | chr2:34...   119   9e-28
Medtr2g081350.1 | calcium-binding EF-hand protein | HC | chr2:34...   116   5e-27
Medtr2g081440.1 | calcium-binding EF-hand protein | HC | chr2:34...   114   1e-26
Medtr4g112460.1 | EF hand calcium-binding family protein | HC | ...    47   3e-06

>Medtr1g052885.1 | calcium-binding EF-hand protein | HC |
           chr1:21941333-21941903 | 20130731
          Length = 113

 Score =  187 bits (474), Expect = 3e-48,   Method: Compositional matrix adjust.
 Identities = 92/105 (87%), Positives = 100/105 (95%)

Query: 14  FEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLGLQDMKEDELVS 73
           FEDLLPVMANKLGGEGLIKELCNGF++LMDK++GVITL+SL+ NAAVLGLQD+KEDELV 
Sbjct: 6   FEDLLPVMANKLGGEGLIKELCNGFELLMDKEKGVITLDSLRQNAAVLGLQDLKEDELVG 65

Query: 74  MIREGDLDGDGALTQMEFCVLMFRLSPELMEESWIWLEEALQHEL 118
           M+ EGDLD DGALTQMEFCVLMFRLSPELMEESW WLEEALQHEL
Sbjct: 66  MMNEGDLDRDGALTQMEFCVLMFRLSPELMEESWFWLEEALQHEL 110


>Medtr2g089210.1 | calcium-binding EF-hand protein | HC |
           chr2:37668116-37667232 | 20130731
          Length = 115

 Score =  186 bits (473), Expect = 3e-48,   Method: Compositional matrix adjust.
 Identities = 90/110 (81%), Positives = 105/110 (95%)

Query: 9   GKGLEFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLGLQDMKE 68
           GKG++FEDLLPV+ANKLGGEGL+KELCNGF +LMDK++GVITLESL+ N+A++GLQD+KE
Sbjct: 5   GKGIDFEDLLPVIANKLGGEGLMKELCNGFKLLMDKEKGVITLESLRKNSALMGLQDLKE 64

Query: 69  DELVSMIREGDLDGDGALTQMEFCVLMFRLSPELMEESWIWLEEALQHEL 118
           DELVSM+REGDLDGDGALT+MEFCVLMFRLSP+LMEESW  LEEAL HEL
Sbjct: 65  DELVSMMREGDLDGDGALTEMEFCVLMFRLSPQLMEESWFLLEEALDHEL 114


>Medtr2g081580.1 | calcium-binding EF-hand protein | HC |
           chr2:34232235-34233533 | 20130731
          Length = 118

 Score =  157 bits (397), Expect = 2e-39,   Method: Compositional matrix adjust.
 Identities = 75/111 (67%), Positives = 94/111 (84%)

Query: 12  LEFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLGLQDMKEDEL 71
           ++F+D LP+MANKLGG+GLI ELCNGF++LMD  +GVIT ESLK N+A+LGLQD+ + EL
Sbjct: 8   VQFQDSLPLMANKLGGDGLIDELCNGFNLLMDSTKGVITFESLKKNSALLGLQDLTDVEL 67

Query: 72  VSMIREGDLDGDGALTQMEFCVLMFRLSPELMEESWIWLEEALQHELDNNY 122
            SMI EGD DGDGAL QMEFCVLMFRLSPELM+ S +WLE+ LQ E+ +++
Sbjct: 68  QSMIVEGDFDGDGALNQMEFCVLMFRLSPELMDGSKMWLEQMLQQEVQDSF 118


>Medtr8g466250.1 | calcium-binding EF-hand protein | HC |
           chr8:23630421-23630973 | 20130731
          Length = 115

 Score =  150 bits (380), Expect = 2e-37,   Method: Compositional matrix adjust.
 Identities = 73/114 (64%), Positives = 88/114 (77%)

Query: 5   GTVNGKGLEFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLGLQ 64
            + N     F D LP MANKLGG+GLI ELCNGF++L D ++GVIT ESLK N+A+LGL 
Sbjct: 2   ASQNPPQSHFYDHLPFMANKLGGDGLIDELCNGFNLLKDSNKGVITFESLKMNSALLGLN 61

Query: 65  DMKEDELVSMIREGDLDGDGALTQMEFCVLMFRLSPELMEESWIWLEEALQHEL 118
              +++L+SM+ EGD DGDGAL QMEFCVLMFRLSPELME S +WLEE LQH+ 
Sbjct: 62  GFSDEDLLSMLMEGDFDGDGALNQMEFCVLMFRLSPELMEGSKLWLEETLQHQF 115


>Medtr2g011400.1 | calcium-binding EF-hand protein | HC |
           chr2:2765951-2766307 | 20130731
          Length = 118

 Score =  139 bits (349), Expect = 8e-34,   Method: Compositional matrix adjust.
 Identities = 67/114 (58%), Positives = 85/114 (74%)

Query: 1   MAGTGTVNGKGLEFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAV 60
           MA +   NG  + FED  P M  KLG EG +KEL NGF++LMD+++ VIT ESLK N+A+
Sbjct: 1   MASSSRKNGGSVVFEDYFPSMMEKLGSEGFMKELANGFNVLMDREKKVITFESLKKNSAL 60

Query: 61  LGLQDMKEDELVSMIREGDLDGDGALTQMEFCVLMFRLSPELMEESWIWLEEAL 114
           LGL+ M +DE+  M+REGD+DGDGAL +MEFC LMFRLSP LM +S   LEEA+
Sbjct: 61  LGLEGMNDDEITCMLREGDIDGDGALNEMEFCTLMFRLSPALMSDSKQLLEEAI 114


>Medtr8g027180.1 | calcium-binding EF-hand protein | HC |
           chr8:9563180-9562497 | 20130731
          Length = 125

 Score =  124 bits (310), Expect = 3e-29,   Method: Compositional matrix adjust.
 Identities = 56/108 (51%), Positives = 84/108 (77%)

Query: 13  EFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLGLQDMKEDELV 72
           EFEDLLP+MA KL  E  + ELC GF++L D++ G+IT ESL+ N+A+LG++ M +++  
Sbjct: 18  EFEDLLPIMAEKLDVETFVSELCGGFNLLADQETGLITSESLRKNSAMLGMEGMSKEDAE 77

Query: 73  SMIREGDLDGDGALTQMEFCVLMFRLSPELMEESWIWLEEALQHELDN 120
           +M+++GDLDGDG L + EFC+LM RLSP +ME++  WLE+A++ +L N
Sbjct: 78  AMVKQGDLDGDGKLNETEFCILMVRLSPGMMEDAETWLEKAIEEQLRN 125


>Medtr2g081300.1 | calcium-binding EF-hand protein | HC |
           chr2:34115260-34114496 | 20130731
          Length = 110

 Score =  119 bits (297), Expect = 9e-28,   Method: Compositional matrix adjust.
 Identities = 59/93 (63%), Positives = 75/93 (80%), Gaps = 1/93 (1%)

Query: 12  LEFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLGLQDMKEDEL 71
           L+FED  P M ++LG EG I ELCNGF +LMD ++G+IT ESLK N  +LGL+ ++++EL
Sbjct: 3   LDFEDYFPSMISRLGAEGFIGELCNGFRLLMDVNKGLITFESLKMNCFMLGLE-VRDEEL 61

Query: 72  VSMIREGDLDGDGALTQMEFCVLMFRLSPELME 104
           V M+ EGDLDGDGAL QMEFC+LMFRLSP LM+
Sbjct: 62  VYMLMEGDLDGDGALNQMEFCILMFRLSPCLMD 94


>Medtr2g081350.1 | calcium-binding EF-hand protein | HC |
           chr2:34144304-34143960 | 20130731
          Length = 114

 Score =  116 bits (291), Expect = 5e-27,   Method: Compositional matrix adjust.
 Identities = 58/98 (59%), Positives = 74/98 (75%), Gaps = 1/98 (1%)

Query: 12  LEFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLGLQDMKEDEL 71
           ++FED  P M   LG EG I EL NGF +LMD ++G+IT ESLK N  +LGL+ ++++EL
Sbjct: 7   MDFEDYFPSMVASLGAEGFIGELYNGFHLLMDANKGLITFESLKMNCFMLGLE-VRDEEL 65

Query: 72  VSMIREGDLDGDGALTQMEFCVLMFRLSPELMEESWIW 109
           V M+ EGDLDGDGAL QMEFC+LMFRLSP LM+   I+
Sbjct: 66  VYMLMEGDLDGDGALNQMEFCILMFRLSPCLMDGPKIY 103


>Medtr2g081440.1 | calcium-binding EF-hand protein | HC |
           chr2:34176425-34176081 | 20130731
          Length = 114

 Score =  114 bits (286), Expect = 1e-26,   Method: Compositional matrix adjust.
 Identities = 58/93 (62%), Positives = 70/93 (75%), Gaps = 1/93 (1%)

Query: 12  LEFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLGLQDMKEDEL 71
           L+FED  P M   LG E  I ELC GF +LMD ++G+IT ESLK N  +LG++ +K+DEL
Sbjct: 7   LDFEDYFPSMVASLGAEAFIGELCYGFHLLMDVNKGLITFESLKMNCFMLGME-VKDDEL 65

Query: 72  VSMIREGDLDGDGALTQMEFCVLMFRLSPELME 104
           V M+ EGDLDGDGAL  MEFC+LMFRLSP LME
Sbjct: 66  VYMLMEGDLDGDGALNPMEFCILMFRLSPCLME 98


>Medtr4g112460.1 | EF hand calcium-binding family protein | HC |
           chr4:46040114-46041145 | 20130731
          Length = 169

 Score = 47.4 bits (111), Expect = 3e-06,   Method: Compositional matrix adjust.
 Identities = 34/99 (34%), Positives = 54/99 (54%), Gaps = 4/99 (4%)

Query: 8   NGKG-LEFEDLLPVMANKLGGEGLI-KELCNGFDMLMDKD-RGVITLESLKTNAAVLGLQ 64
           NG G +EF++L+  +   +  E L+ +E   G     D+D  G I+   L    A +G Q
Sbjct: 58  NGNGSVEFDELVEAIMPNMNAEVLVNQEQLIGVFKCFDRDGNGFISAAELAGAMAKMG-Q 116

Query: 65  DMKEDELVSMIREGDLDGDGALTQMEFCVLMFRLSPELM 103
            +   EL+ MIRE D+DGDG ++  EF  +M R + +L+
Sbjct: 117 PLTYKELIEMIREADMDGDGVISFSEFATIMARSASDLL 155