Miyakogusa Predicted Gene
- Lj1g3v2294860.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj1g3v2294860.1 tr|F9W2W3|F9W2W3_SOYBN Alpha-amylase OS=Glycine
max GN=AMY1 PE=4 SV=1,86.36,0,no description,Immunoglobulin-like fold;
no description,Glycoside hydrolase, catalytic domain; no
de,CUFF.28875.1
(848 letters)
Database: TAIR10_pep
35,386 sequences; 14,482,855 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G03650.1 | Symbols: SBE2.2 | starch branching enzyme 2.2 | ch... 806 0.0
AT2G36390.1 | Symbols: SBE2.1, BE3 | starch branching enzyme 2.1... 797 0.0
AT3G20440.2 | Symbols: EMB2729, BE1 | Alpha amylase family prote... 445 e-125
AT3G20440.3 | Symbols: EMB2729 | Alpha amylase family protein | ... 437 e-122
AT3G20440.1 | Symbols: EMB2729, BE1 | Alpha amylase family prote... 402 e-112
AT1G03310.1 | Symbols: ATISA2, ISA2, DBE1, BE2 | debranching enz... 56 1e-07
AT1G03310.2 | Symbols: ATISA2, ISA2, DBE1, BE2 | debranching enz... 56 1e-07
AT2G39930.1 | Symbols: ISA1, ATISA1 | isoamylase 1 | chr2:166660... 54 5e-07
AT4G09020.1 | Symbols: ATISA3, ISA3 | isoamylase 3 | chr4:578409... 52 2e-06
>AT5G03650.1 | Symbols: SBE2.2 | starch branching enzyme 2.2 |
chr5:931924-937470 FORWARD LENGTH=805
Length = 805
Score = 806 bits (2082), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 387/689 (56%), Positives = 493/689 (71%), Gaps = 26/689 (3%)
Query: 87 ILHIDPAIKPFKDHFKCRLKRYIDQKKLIEEYEGGLEEFAQGYLKFGFNREEGGIVYREW 146
I IDP ++ + +H R +Y ++ I++YEGGLE F++GY K GF+R + GI YREW
Sbjct: 125 IYEIDPMLRTYNNHLDYRYGQYKRLREEIDKYEGGLEAFSRGYEKLGFSRSDAGITYREW 184
Query: 147 APAAQEAQIIGDFNEWNGSNHPMEKNQFGVWSIKIPD-VAGNPAIPHNSRVKFRFRHGGV 205
AP A+ A +IGDFN WN + M +N+FGVW I +P+ G+PAIPH SRVK R
Sbjct: 185 APGAKAASLIGDFNNWNSNADIMTRNEFGVWEIFLPNNTDGSPAIPHGSRVKIRMDTPSG 244
Query: 206 WADRIPAWIKYATVDPTKFAAPYDGVYWDPPLSERYQFKYXXXXXXXXXXIYEAHVGMSS 265
D IPAWIK++ P + P++G+Y+DPP E+Y FK+ IYEAHVGMSS
Sbjct: 245 IKDSIPAWIKFSVQAPGEI--PFNGIYYDPPEEEKYVFKHPQPKRPKSLRIYEAHVGMSS 302
Query: 266 SEPRINSYKEFADDILPRIRANNYNTVQLMAVMEHSYYASFGYHVTNFFAVSSRSGTPED 325
+EP +N+Y F DD+LPRI+ YN VQ+MA+ EHSYYASFGYHVTNFFA SSR GTPE+
Sbjct: 303 TEPMVNTYANFRDDVLPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRCGTPEE 362
Query: 326 LKYLIDKAHSLGLHVLMDVVHSHASNNVTDGLNGFDVGQVSQESYFHTGDRGYHKLWDSR 385
LK LID+AH LGL VLMD+VHSHAS N DGLN FD + YFH+G RGYH +WDSR
Sbjct: 363 LKSLIDRAHELGLVVLMDIVHSHASKNTLDGLNMFDG---TDAHYFHSGPRGYHWMWDSR 419
Query: 386 LFNYANWEVLRFLLSNLRWWLEEFKFDGFRFDGVTSMLYHHHGVNIAFSGDYNEYFSEAT 445
LFNY +WEVLR+LLSN RWWLEE+KFDGFRFDGVTSM+Y HHG+++ F+G+Y EYF T
Sbjct: 420 LFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLSVGFTGNYTEYFGLET 479
Query: 446 DVDAVVYLMLANSLIHNILPDATVIAEDVSGMPGLGRPISEVGIGFDYRLAMAIPDKWID 505
DVDAV YLML N +IH + P+A + EDVSGMP P+ + G+GFDYRL MAI DKWI+
Sbjct: 480 DVDAVNYLMLVNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIE 539
Query: 506 YLKNKKDHEWSMKEISLSLTNRRYSEKCVSYAESHDQSIVGDKTFSFLLMDEEIYSGMSC 565
LK K+D +W M +I +LTNRR+SEKC+SYAESHDQ++VGDKT +F LMD+++Y M+
Sbjct: 540 MLK-KRDEDWQMGDIIYTLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKDMYDFMAV 598
Query: 566 LADASPTIERGIALHKMIHFITMSLGGEGYLNFMGNEFGHPEWIDFPR-----------E 614
++P I+RGIALHKMI ITM LGGEGYLNFMGNEFGHPEWIDFPR
Sbjct: 599 DRPSTPLIDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRGEQRLSDGSVIP 658
Query: 615 GNGWSYEKCRRQWSLVDTDHLRYKFMNAFDKAMNLLDDKFSFLASTKQIVSSTNEEDKVI 674
GN +SY+KCRR++ L D D+LRY+ + FD+AM L++ + F+ S Q +S +E D+VI
Sbjct: 659 GNNFSYDKCRRRFDLGDADYLRYRGLQEFDQAMQHLEENYGFMTSEHQFISRKDEADRVI 718
Query: 675 VFERGDLVFVFNFHPETTYEGYKVGCDLPGKYRVALDSDAREFGGHGRVGHNVDHFTAPE 734
VFERGDLVFVFNFH ++Y Y++GC PGKY++ LDSD FGG R+ ++FT +
Sbjct: 719 VFERGDLVFVFNFHWTSSYFDYRIGCSKPGKYKIVLDSDDPLFGGFNRLDRKAEYFTY-D 777
Query: 735 GIPGVPESNFNNRPNSFKILSPPRTCVVY 763
G+ ++ RP SF + +P RT VVY
Sbjct: 778 GL-------YDERPCSFMVYAPCRTAVVY 799
>AT2G36390.1 | Symbols: SBE2.1, BE3 | starch branching enzyme 2.1 |
chr2:15264283-15269940 FORWARD LENGTH=858
Length = 858
Score = 797 bits (2058), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 384/685 (56%), Positives = 487/685 (71%), Gaps = 26/685 (3%)
Query: 87 ILHIDPAIKPFKDHFKCRLKRYIDQKKLIEEYEGGLEEFAQGYLKFGFNREEGGIVYREW 146
I IDP + ++H R +Y ++ I++ EGGLE F++GY FGF R GI YREW
Sbjct: 160 IYDIDPMLNSHRNHLDYRYGQYRKLREEIDKNEGGLEAFSRGYEIFGFTRSATGITYREW 219
Query: 147 APAAQEAQIIGDFNEWNGSNHPMEKNQFGVWSIKIPDVA-GNPAIPHNSRVKFRFRHGGV 205
AP A+ A +IGDFN WN + M +N FGVW I +P+ A G+PAIPH SRVK R
Sbjct: 220 APGAKAASLIGDFNNWNAKSDVMARNDFGVWEIFLPNNADGSPAIPHGSRVKIRMDTPSG 279
Query: 206 WADRIPAWIKYATVDPTKFAAPYDGVYWDPPLSERYQFKYXXXXXXXXXXIYEAHVGMSS 265
D IPAWIKY+ P + PY+GVY+DPP ++Y FK+ IYE+HVGMSS
Sbjct: 280 IKDSIPAWIKYSVQPPGEI--PYNGVYYDPPEEDKYAFKHPRPKKPTSLRIYESHVGMSS 337
Query: 266 SEPRINSYKEFADDILPRIRANNYNTVQLMAVMEHSYYASFGYHVTNFFAVSSRSGTPED 325
+EP+IN+Y F DD+LPRI+ YN VQ+MA+ EH+YYASFGYHVTNFFA SSR GTP+D
Sbjct: 338 TEPKINTYANFRDDVLPRIKKLGYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDD 397
Query: 326 LKYLIDKAHSLGLHVLMDVVHSHASNNVTDGLNGFDVGQVSQESYFHTGDRGYHKLWDSR 385
LK LIDKAH LGL VLMD+VHSHAS N DGL+ FD G Q YFH+G RGYH +WDSR
Sbjct: 398 LKSLIDKAHELGLVVLMDIVHSHASKNTLDGLDMFD-GTDGQ--YFHSGSRGYHWMWDSR 454
Query: 386 LFNYANWEVLRFLLSNLRWWLEEFKFDGFRFDGVTSMLYHHHGVNIAFSGDYNEYFSEAT 445
LFNY +WEVLR+LLSN RWWLEE+KFDGFRFDGVTSM+Y HHG+ + F+G+YNEYF +T
Sbjct: 455 LFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEYFGYST 514
Query: 446 DVDAVVYLMLANSLIHNILPDATVIAEDVSGMPGLGRPISEVGIGFDYRLAMAIPDKWID 505
DVDAVVYLML N LIH + P+A V+ EDVSGMP P+ + G+GFDYRL MA+ DKWI+
Sbjct: 515 DVDAVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVPVEDGGVGFDYRLHMAVADKWIE 574
Query: 506 YLKNKKDHEWSMKEISLSLTNRRYSEKCVSYAESHDQSIVGDKTFSFLLMDEEIYSGMSC 565
LK K+D +W + +I+ +LTNRR+ EKCV YAESHDQ++VGDKT +F LMD+++Y M+
Sbjct: 575 LLK-KRDEDWQVGDITFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAV 633
Query: 566 LADASPTIERGIALHKMIHFITMSLGGEGYLNFMGNEFGHPEWIDFPRE----------- 614
A+P ++RGIALHKMI ITM LGGEGYLNFMGNEFGHPEWIDFPR
Sbjct: 634 DRQATPRVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRTDQHLPDGRVIA 693
Query: 615 GNGWSYEKCRRQWSLVDTDHLRYKFMNAFDKAMNLLDDKFSFLASTKQIVSSTNEEDKVI 674
GN SY+KCRR++ L D ++LRY + FD+AM L++ + F+ S Q +S +E D+VI
Sbjct: 694 GNNGSYDKCRRRFDLGDAEYLRYHGLQEFDRAMQNLEETYGFMTSEHQYISRKDEGDRVI 753
Query: 675 VFERGDLVFVFNFHPETTYEGYKVGCDLPGKYRVALDSDAREFGGHGRVGHNVDHFTAPE 734
VFERG+L+FVFNFH +Y Y++GC +PGKY++ LDSD FGG R+ + + FT+
Sbjct: 754 VFERGNLLFVFNFHWTNSYSDYRIGCSVPGKYKIVLDSDNSLFGGFNRLDDSAEFFTS-- 811
Query: 735 GIPGVPESNFNNRPNSFKILSPPRT 759
+ ++RP SF + +P RT
Sbjct: 812 ------DGRHDDRPCSFMVYAPCRT 830
>AT3G20440.2 | Symbols: EMB2729, BE1 | Alpha amylase family protein
| chr3:7123603-7130364 REVERSE LENGTH=899
Length = 899
Score = 445 bits (1144), Expect = e-125, Method: Compositional matrix adjust.
Identities = 231/581 (39%), Positives = 350/581 (60%), Gaps = 28/581 (4%)
Query: 188 PAIPHNSRVKFRFRHGGVWADRIPAWIKYATVDPTKFAAPYDGVYWDPPLSERYQFKYXX 247
PA+PH S+ + F +R+PAW Y V P ++W+P Y++KY
Sbjct: 334 PAVPHGSKYRLYFNTPDGPLERVPAWATY--VQPEDEGKQAYAIHWEPSPEAAYKWKYSK 391
Query: 248 XXXXXXXXIYEAHVGMSSSEPRINSYKEFADDILPRIRANNYNTVQLMAVMEHSYYASFG 307
IYE HVG+S SEP++++++EF +LP ++ YN +QL+ V EH Y + G
Sbjct: 392 PKVPESLRIYECHVGISGSEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVG 451
Query: 308 YHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLHVLMDVVHSHASNNVTDGLNGFDVGQVSQ 367
Y VTNFFA SSR GTP+D K L+D+AH LGL V +D+VHS+A+ + GL+ FD S
Sbjct: 452 YRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD---GSN 508
Query: 368 ESYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEFKFDGFRFDGVTSMLYHHH 427
+ YFH G RG+HK W +R+F Y + +VL FL+SNL WW+ E++ DG++F + SM+Y H+
Sbjct: 509 DCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHN 568
Query: 428 GVNIAFSGDYNEYFSEATDVDAVVYLMLANSLIHNILPDATVIAEDVSGMPGLGRPISEV 487
G +F+ D ++Y ++ D DA++YL+LAN ++H P+ IAED + PGL P+S+
Sbjct: 569 GF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEPVSQG 627
Query: 488 GIGFDYRLAMAIPDKWIDYLKNKKDHEWSMKEISLSLT-NRRYSEKCVSYAESHDQSIVG 546
G+GFDY + ++ + W+ L N D+EWSM +I +L N+ Y++K +SYAE+H+QSI G
Sbjct: 628 GLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQSISG 687
Query: 547 DKTFSFLLMDEEIYSGMSCLADASP----TIERGIALHKMIHFITMSLGGEGYLNFMGNE 602
++F+ E ++ G+ + SP ++RGI+LHKMI IT + GG YLNFMGNE
Sbjct: 688 GRSFA-----EILFGGVD---NGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNFMGNE 739
Query: 603 FGHPEWIDFPREGNGWSYEKCRRQWSLVDTDHLRYKFMNAFDKAMNLLDDKFSFLASTKQ 662
FGHPE ++FP + N +S+ R+W L+++ + F +FDK + LD L+
Sbjct: 740 FGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLF--SFDKELMDLDKSKGILSRGLP 797
Query: 663 IVSSTNEEDKVIVFERGDLVFVFNFHPETTYEGYKVGCDLPGKYRVALDSDAREFGGHGR 722
+ N+ + VI F RG +F+FNFHP +YE Y VG + G+Y + L+SD ++GG G
Sbjct: 798 SIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVKYGGQGI 857
Query: 723 VGHNVDHFTAPEGIPGVPESNFNNRPNSFKILSPPRTCVVY 763
V DH+ + + + N ++ P RT VY
Sbjct: 858 VTE--DHY-----LQRSISKRIDGQRNCLEVFLPSRTAQVY 891
>AT3G20440.3 | Symbols: EMB2729 | Alpha amylase family protein |
chr3:7123603-7130364 REVERSE LENGTH=897
Length = 897
Score = 437 bits (1123), Expect = e-122, Method: Compositional matrix adjust.
Identities = 226/553 (40%), Positives = 339/553 (61%), Gaps = 26/553 (4%)
Query: 188 PAIPHNSRVKFRFRHGGVWADRIPAWIKYATVDPTKF---AAPYDGVYWDPPLSERYQFK 244
PA+PH S+ + F +R+PAW Y T F ++W+P Y++K
Sbjct: 334 PAVPHGSKYRLYFNTPDGPLERVPAWATYVQPGMTAFEDEGKQAYAIHWEPSPEAAYKWK 393
Query: 245 YXXXXXXXXXXIYEAHVGMSSSEPRINSYKEFADDILPRIRANNYNTVQLMAVMEHSYYA 304
Y IYE HVG+S SEP++++++EF +LP ++ YN +QL+ V EH Y
Sbjct: 394 YSKPKVPESLRIYECHVGISGSEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYF 453
Query: 305 SFGYHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLHVLMDVVHSHASNNVTDGLNGFDVGQ 364
+ GY VTNFFA SSR GTP+D K L+D+AH LGL V +D+VHS+A+ + GL+ FD
Sbjct: 454 TVGYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD--- 510
Query: 365 VSQESYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEFKFDGFRFDGVTSMLY 424
S + YFH G RG+HK W +R+F Y + +VL FL+SNL WW+ E++ DG++F + SM+Y
Sbjct: 511 GSNDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIY 570
Query: 425 HHHGVNIAFSGDYNEYFSEATDVDAVVYLMLANSLIHNILPDATVIAEDVSGMPGLGRPI 484
H+G +F+ D ++Y ++ D DA++YL+LAN ++H P+ IAED + PGL P+
Sbjct: 571 THNGF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEPV 629
Query: 485 SEVGIGFDYRLAMAIPDKWIDYLKNKKDHEWSMKEISLSLT-NRRYSEKCVSYAESHDQS 543
S+ G+GFDY + ++ + W+ L N D+EWSM +I +L N+ Y++K +SYAE+H+QS
Sbjct: 630 SQGGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQS 689
Query: 544 IVGDKTFSFLLMDEEIYSGMSCLADASP----TIERGIALHKMIHFITMSLGGEGYLNFM 599
I G ++F+ E ++ G+ + SP ++RGI+LHKMI IT + GG YLNFM
Sbjct: 690 ISGGRSFA-----EILFGGVD---NGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNFM 741
Query: 600 GNEFGHPEWIDFPREGNGWSYEKCRRQWSLVDTDHLRYKFMNAFDKAMNL--LDDKFSFL 657
GNEFGHPE ++FP + N +S+ R+W L+++ + F +FDK L LD L
Sbjct: 742 GNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLF--SFDKVSELMDLDKSKGIL 799
Query: 658 ASTKQIVSSTNEEDKVIVFERGDLVFVFNFHPETTYEGYKVGCDLPGKYRVALDSDAREF 717
+ + N+ + VI F RG +F+FNFHP +YE Y VG + G+Y + L+SD ++
Sbjct: 800 SRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVKY 859
Query: 718 GGHGRVGHNVDHF 730
GG G V DH+
Sbjct: 860 GGQGIVTE--DHY 870
>AT3G20440.1 | Symbols: EMB2729, BE1 | Alpha amylase family protein
| chr3:7123603-7130364 REVERSE LENGTH=869
Length = 869
Score = 402 bits (1032), Expect = e-112, Method: Compositional matrix adjust.
Identities = 219/581 (37%), Positives = 332/581 (57%), Gaps = 58/581 (9%)
Query: 188 PAIPHNSRVKFRFRHGGVWADRIPAWIKYATVDPTKFAAPYDGVYWDPPLSERYQFKYXX 247
PA+PH S+ + F +R+PAW Y V P ++W+P Y++KY
Sbjct: 334 PAVPHGSKYRLYFNTPDGPLERVPAWATY--VQPEDEGKQAYAIHWEPSPEAAYKWKYSK 391
Query: 248 XXXXXXXXIYEAHVGMSSSEPRINSYKEFADDILPRIRANNYNTVQLMAVMEHSYYASFG 307
IYE HVG+S SEP++++++EF
Sbjct: 392 PKVPESLRIYECHVGISGSEPKVSTFEEFTKK---------------------------- 423
Query: 308 YHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLHVLMDVVHSHASNNVTDGLNGFDVGQVSQ 367
VTNFFA SSR GTP+D K L+D+AH LGL V +D+VHS+A+ + GL+ FD S
Sbjct: 424 --VTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFDG---SN 478
Query: 368 ESYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEFKFDGFRFDGVTSMLYHHH 427
+ YFH G RG+HK W +R+F Y + +VL FL+SNL WW+ E++ DG++F + SM+Y H+
Sbjct: 479 DCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHN 538
Query: 428 GVNIAFSGDYNEYFSEATDVDAVVYLMLANSLIHNILPDATVIAEDVSGMPGLGRPISEV 487
G +F+ D ++Y ++ D DA++YL+LAN ++H P+ IAED + PGL P+S+
Sbjct: 539 GF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEPVSQG 597
Query: 488 GIGFDYRLAMAIPDKWIDYLKNKKDHEWSMKEISLSLT-NRRYSEKCVSYAESHDQSIVG 546
G+GFDY + ++ + W+ L N D+EWSM +I +L N+ Y++K +SYAE+H+QSI G
Sbjct: 598 GLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQSISG 657
Query: 547 DKTFSFLLMDEEIYSGMSCLADASP----TIERGIALHKMIHFITMSLGGEGYLNFMGNE 602
++F+ E ++ G+ + SP ++RGI+LHKMI IT + GG YLNFMGNE
Sbjct: 658 GRSFA-----EILFGGVD---NGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNFMGNE 709
Query: 603 FGHPEWIDFPREGNGWSYEKCRRQWSLVDTDHLRYKFMNAFDKAMNLLDDKFSFLASTKQ 662
FGHPE ++FP + N +S+ R+W L+++ + F +FDK + LD L+
Sbjct: 710 FGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLF--SFDKELMDLDKSKGILSRGLP 767
Query: 663 IVSSTNEEDKVIVFERGDLVFVFNFHPETTYEGYKVGCDLPGKYRVALDSDAREFGGHGR 722
+ N+ + VI F RG +F+FNFHP +YE Y VG + G+Y + L+SD ++GG G
Sbjct: 768 SIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVKYGGQGI 827
Query: 723 VGHNVDHFTAPEGIPGVPESNFNNRPNSFKILSPPRTCVVY 763
V DH+ + + + N ++ P RT VY
Sbjct: 828 VTE--DHY-----LQRSISKRIDGQRNCLEVFLPSRTAQVY 861
>AT1G03310.1 | Symbols: ATISA2, ISA2, DBE1, BE2 | debranching enzyme
1 | chr1:813975-816623 FORWARD LENGTH=882
Length = 882
Score = 56.2 bits (134), Expect = 1e-07, Method: Compositional matrix adjust.
Identities = 34/108 (31%), Positives = 60/108 (55%), Gaps = 11/108 (10%)
Query: 326 LKYLIDKAHSLGLHVLMDVVHSHASNNVTDGLNGFDVGQVSQESYFHTGDRGYHKLWDSR 385
+K ++ K HS G+ VL++VV +H ++ + L G D SY++ +G DS+
Sbjct: 448 MKVMVKKLHSEGIEVLLEVVFTHTAD--SGALRGID-----DSSYYY---KGRANDLDSK 497
Query: 386 LFNYANWEVLR-FLLSNLRWWLEEFKFDGFRFDGVTSMLYHHHGVNIA 432
+ N+ V++ +L +LR+W+ EF DGF F +S+L HG ++
Sbjct: 498 SYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLS 545
>AT1G03310.2 | Symbols: ATISA2, ISA2, DBE1, BE2 | debranching enzyme
1 | chr1:813975-816623 FORWARD LENGTH=882
Length = 882
Score = 56.2 bits (134), Expect = 1e-07, Method: Compositional matrix adjust.
Identities = 34/108 (31%), Positives = 60/108 (55%), Gaps = 11/108 (10%)
Query: 326 LKYLIDKAHSLGLHVLMDVVHSHASNNVTDGLNGFDVGQVSQESYFHTGDRGYHKLWDSR 385
+K ++ K HS G+ VL++VV +H ++ + L G D SY++ +G DS+
Sbjct: 448 MKVMVKKLHSEGIEVLLEVVFTHTAD--SGALRGID-----DSSYYY---KGRANDLDSK 497
Query: 386 LFNYANWEVLR-FLLSNLRWWLEEFKFDGFRFDGVTSMLYHHHGVNIA 432
+ N+ V++ +L +LR+W+ EF DGF F +S+L HG ++
Sbjct: 498 SYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLS 545
>AT2G39930.1 | Symbols: ISA1, ATISA1 | isoamylase 1 |
chr2:16666078-16672183 FORWARD LENGTH=783
Length = 783
Score = 53.5 bits (127), Expect = 5e-07, Method: Compositional matrix adjust.
Identities = 54/193 (27%), Positives = 81/193 (41%), Gaps = 33/193 (17%)
Query: 256 IYEAHV-GMSSSEPR----INSYKEFADDILPRIRANNYNTVQLMAVME------HSYYA 304
IYE HV G + E +Y+ A+ L ++ N ++LM E +SY
Sbjct: 220 IYEMHVRGFTRHESSKIEFPGTYQGVAEK-LDHLKELGINCIELMPCHEFNELEYYSYNT 278
Query: 305 SFGYHVTNFFAVSS--------RSGTPE----------DLKYLIDKAHSLGLHVLMDVVH 346
G H NF+ S+ R + + K L+ +AH G+ V+MDVV
Sbjct: 279 ILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVL 338
Query: 347 SHASNNVTDGLNGFDVGQVSQESYFHTGDRG--YHKLWDSRLFNYANWEVLRFLLSNLRW 404
+H + G F V Y+ +G Y+ FN + V +F+L LR+
Sbjct: 339 NHTAEGNEKG-PIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRY 397
Query: 405 WLEEFKFDGFRFD 417
W+ E DGFRFD
Sbjct: 398 WVTEMHVDGFRFD 410
>AT4G09020.1 | Symbols: ATISA3, ISA3 | isoamylase 3 |
chr4:5784099-5788839 FORWARD LENGTH=764
Length = 764
Score = 52.0 bits (123), Expect = 2e-06, Method: Compositional matrix adjust.
Identities = 50/186 (26%), Positives = 81/186 (43%), Gaps = 41/186 (22%)
Query: 264 SSSEPRIN-SYKEFADDILPRIRANNYNTVQLMAVMEHS-------------YYASFGYH 309
S +P I SY F + I P ++ N V+L+ V E ++GY
Sbjct: 252 SGMDPAIGGSYLGFIEKI-PHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYS 310
Query: 310 VTNFFAVSSRSGTPE--------DLKYLIDKAHSLGLHVLMDVVHSHASNNVTDGLNGFD 361
NFFA SR + E + K ++ HS G+ V++DVV++H N D
Sbjct: 311 TVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHT--------NEAD 362
Query: 362 VGQVSQESYFHTGDRGYHKL-WDSRLFNYANWE---------VLRFLLSNLRWWLEEFKF 411
S+ ++ Y+ L +++L N++ V+ +L +LR W+ E+
Sbjct: 363 DKYPYTTSFRGIDNKVYYMLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHV 422
Query: 412 DGFRFD 417
DGFRFD
Sbjct: 423 DGFRFD 428