Miyakogusa Predicted Gene

Lj0g3v0277769.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0277769.1 tr|G7LEC2|G7LEC2_MEDTR Gamma-tubulin complex
component OS=Medicago truncatula GN=MTR_8g011450 PE=4
S,88.69,0,Spc97_Spc98,Spc97/Spc98; GAMMA-TUBULIN COMPLEX COMPONENT 2
(GCP-2),NULL; GAMMA TUBULIN COMPLEX PROTE,CUFF.18456.1
         (654 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g011450.1 | Spc97/Spc98 family of spindle pole body (SBP) ...  1123   0.0  
Medtr7g093560.1 | tubulin gamma complex-associated protein | HC ...   140   4e-33
Medtr3g101230.1 | Spc97/Spc98 family of spindle pole body (SBP) ...    52   2e-06

>Medtr8g011450.1 | Spc97/Spc98 family of spindle pole body (SBP)
           component | HC | chr8:3076171-3085297 | 20130731
          Length = 664

 Score = 1123 bits (2906), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 543/656 (82%), Positives = 576/656 (87%), Gaps = 2/656 (0%)

Query: 1   MNQSTT-GFQNPIGYYDPAVQELIVIDDVLSAMVAVEGRYILIKTVRGKKDDV-TFLVDP 58
           MNQ+   GF+N IGYYDP VQELIVID+VLSAMV VEGRYI IKT+RGKKD++  FLVDP
Sbjct: 1   MNQTAAAGFENLIGYYDPPVQELIVIDEVLSAMVGVEGRYISIKTLRGKKDEIINFLVDP 60

Query: 59  SMDLALQELAKRIFPLCRSYLLINQFVESRSQFQNGLVNHAFSAALRALLIDYQAMVAQL 118
           SMDLALQELAKRIFPLCRS+LLINQFVESRSQF+NGLVNHAFSAALRA L+DYQAMVAQL
Sbjct: 61  SMDLALQELAKRIFPLCRSFLLINQFVESRSQFENGLVNHAFSAALRAFLLDYQAMVAQL 120

Query: 119 EHQFRLGRLSLQGLWFYCQPMMRSMQALSTVIQMASVNNFSGSAVLNLLQSQAKAMAGDN 178
           EHQFRLGRLSLQGLWFYCQPM+RSMQALSTVIQ ASVNNF+GSAVLNLLQSQAKAMAGDN
Sbjct: 121 EHQFRLGRLSLQGLWFYCQPMLRSMQALSTVIQKASVNNFTGSAVLNLLQSQAKAMAGDN 180

Query: 179 AVRLLLDKMAQCASSAYMSILERWVYEGVIDDPYGEFFIAEDKSLQKDSLTQDYEAKYWR 238
           AVRLLL+KM QCAS AYMSILERWVYEGVIDDPYGEFFIAEDKSLQK+SLT+DY+AKYWR
Sbjct: 181 AVRLLLEKMTQCASRAYMSILERWVYEGVIDDPYGEFFIAEDKSLQKESLTKDYDAKYWR 240

Query: 239 QRYSLKDGIPSFLANIAGTILTTGKYLNVMRECGHNVQVPPSENSKLMSLGSNHHYLECI 298
           QRYSLKDGIPSFLAN AGTILTTGKYLNV+RECGHNVQVPPSENSKLMS GSNHHYLECI
Sbjct: 241 QRYSLKDGIPSFLANSAGTILTTGKYLNVLRECGHNVQVPPSENSKLMSFGSNHHYLECI 300

Query: 299 KAAYNFASGELLNLIKEKYDLTGRLRSIKHYLLLDQGDFLVHFMDIARDELAKKPDEVSV 358
           KAAYNFASGEL+NLIKEKYDLTG+LRSIKHYLLLDQGDFLVHFMDIARDELAKKPDEVSV
Sbjct: 301 KAAYNFASGELVNLIKEKYDLTGKLRSIKHYLLLDQGDFLVHFMDIARDELAKKPDEVSV 360

Query: 359 EKLQSXXXXXXXXXXXXXXPFHEGLTCVVERSSLLKRLGTFNDLEVSQRNSGNNDLLEEP 418
           EKLQS              P+HEGLTC+VERSSLLKRLGTFN  EV+QR+  NND+LEEP
Sbjct: 361 EKLQSLLDLALRTTAAAVDPYHEGLTCIVERSSLLKRLGTFNVTEVNQRDVINNDILEEP 420

Query: 419 VSITCLETFSLSYKVHWPLSIVLSRKALTKYQLIFRFLFHCKHVDRQLCGAWQVHQGVRA 478
           VSIT LETFSLSYK HWPLSIVLSRKALTKYQLIFRFLFHCKHVDRQLCGAWQ HQGVRA
Sbjct: 421 VSITGLETFSLSYKAHWPLSIVLSRKALTKYQLIFRFLFHCKHVDRQLCGAWQTHQGVRA 480

Query: 479 LNTHGTAISRSSLLCRSMLKFINSLLHYLTFEVIEPNWHLMYNRLQSANSIDEVIQHHDF 538
           LNT GTAISRSSLLCRSMLKF+NSLLHYLTFEVIEPNWH+MY RLQSANSIDEVIQHHDF
Sbjct: 481 LNTRGTAISRSSLLCRSMLKFVNSLLHYLTFEVIEPNWHMMYTRLQSANSIDEVIQHHDF 540

Query: 539 FXXXXXXXXXXXXXXXXXXXXXXXSMCLQYAAATQWLISSSIVLHSPEEPNDDSVRSKQR 598
           F                       SMCLQYAAATQWLISSSIVLHSP+EP  DS  +KQ 
Sbjct: 541 FLDKCLRECLLLLPELLKKVERLKSMCLQYAAATQWLISSSIVLHSPDEPKVDSTGAKQS 600

Query: 599 KSGQILKSTTNNAAVTDSVLKFEKEFNAEXXXXXXXXXXXXHAEPYLAHLAQWILG 654
           KSGQ+LKSTT NAAVT+S+LKFEKEF+AE             AEPYLAHLA+W+LG
Sbjct: 601 KSGQVLKSTTRNAAVTESILKFEKEFDAELQSLGSILSSSSQAEPYLAHLAKWVLG 656


>Medtr7g093560.1 | tubulin gamma complex-associated protein | HC |
           chr7:37201001-37197287 | 20130731
          Length = 841

 Score =  140 bits (353), Expect = 4e-33,   Method: Compositional matrix adjust.
 Identities = 148/560 (26%), Positives = 250/560 (44%), Gaps = 85/560 (15%)

Query: 19  VQELIVIDDVLSAMVAVEGRYILIKTVRGKKDDVTFLVDPSMDLALQELAKRIFPLCRSY 78
           V E  ++ DV+ A   V+G+Y+       ++ D   L+D S+ ++ +  +  +F LC   
Sbjct: 181 VTEEAMVTDVIYACQGVDGKYVKFD----EESDGYVLLD-SIRVS-RASSSMVFKLCELG 234

Query: 79  LLINQ---FVE-SRSQFQN---GLVNHAFSAALRALLIDYQAMVAQLEHQ---------- 121
           +L  +   F+E S  +F     G V  AF AAL+  L DY  ++A LE Q          
Sbjct: 235 VLFKRVVGFIEMSLGRFPAEDVGTVGQAFCAALQDELSDYYKLLAVLEAQSSNPIPLLSE 294

Query: 122 -FRLGR-LSLQGLWFYCQPMMRSMQALSTVIQMASVNNFSGSAVLNLLQSQAKAMAGDNA 179
               G  LSL+ L  +    M  M+ ++ +++   V    G A+   +   A+   GD  
Sbjct: 295 GVSSGNYLSLRRLAVWLAEPMVKMKLMADLVEKCRV--LRGGAMAGAIHLHARH--GDPM 350

Query: 180 VRLLLDKMAQCASSAYMSILERWVYEGVIDDPYGEFFIAEDKSLQKDSLTQDYEAKYWRQ 239
           V   + ++ +   S    +++RWV EG ++D + EFFI   + ++ +SL        WR+
Sbjct: 351 VHEFMKRLLRRVCSPLFEMVKRWVLEGELEDIFVEFFIV-GQPVKAESL--------WRE 401

Query: 240 RYSLKDG-IPSFL-ANIAGTILTTGKYLNVMRECGHNV--QVPPSENSKLMSLGSNHHYL 295
            Y L D  +PSF+ A++A  IL TGK +N +R C  +       +E++  M+      Y 
Sbjct: 402 GYRLYDAMLPSFISASLAQRILRTGKSINFLRVCCEDRGWARAATEDTGAMARRGGFGYG 461

Query: 296 EC------IKAAYNFASGELLNLIKEKYDLTGRLRSIKHYLLLDQGDFLVHFMDIARDEL 349
           E       +  A       LL++I E+Y       +IK YLLL QGDF+ + MDI   EL
Sbjct: 462 ETDTLESLVDEASKRIDKHLLDVIYERYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPEL 521

Query: 350 AKKPDEVSVEKLQSXXXXXXXXXXXXXXPFHEGLTCVVERSSLLKRLGTFNDLEVSQRNS 409
           +   + +S  KL                    GL     R+S       ++D ++  R  
Sbjct: 522 SVPANTISSFKLA-------------------GLLETAIRAS----NAQYDDPDILDRLR 558

Query: 410 GNNDLLEEPVSITCLETFSLSYKVHWPLSIVLSRKALTKYQLIFRFLFHCKHVDRQLCGA 469
               ++         + FSL Y    PL  V +   + +Y  IF FL+  K V+  L GA
Sbjct: 559 VK--MMPHESGDRGWDVFSLEYDARVPLDTVFTESVMARYLRIFNFLWKLKRVEHALIGA 616

Query: 470 WQVHQ-GVRALNTHG-----------TAISRSSLLCRSMLKFINSLLHYLTFEVIEPNWH 517
           W+  +      NT             +A+ R  +L   +  FI++L +Y+ FEV+E +W 
Sbjct: 617 WKTMKPNCITSNTFNRLQHAVKMQLVSALRRCQVLWVEINHFISNLQYYIMFEVLEISWS 676

Query: 518 LMYNRLQSANSIDEVIQHHD 537
              + ++ A  +D+++  H+
Sbjct: 677 NFLSEMEVAKDLDDLLAAHE 696


>Medtr3g101230.1 | Spc97/Spc98 family of spindle pole body (SBP)
           component | HC | chr3:46567256-46577932 | 20130731
          Length = 1001

 Score = 51.6 bits (122), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 54/236 (22%), Positives = 95/236 (40%), Gaps = 16/236 (6%)

Query: 309 LLNLIKEKYDLTGRLRSIKHYLLLDQGDFLVHFMDIARDELAKKP---DEVSVEKLQSXX 365
           LL L+ E + L   L  ++   LL  GD L HF  +  D+L K     D+  +  +    
Sbjct: 652 LLKLMNE-WRLMEELAVLRAIYLLGSGDLLQHFSTVIFDKLDKGETWDDDFELNTILQES 710

Query: 366 XXXXXXXXXXXXPFHEGLTCVVERSSLLKRLGTFNDLEVSQRNS--GNNDLLEEPVSITC 423
                       P  + L   + ++ +       +D E S   S  G +      + +  
Sbjct: 711 IRNSADCMLLSAP--DSLVVSITKNIVE------SDGEASTSGSVLGTHKSRVNNLGMNG 762

Query: 424 LETFSLSYKVHWPLSIVLSRKALTKYQLIFRFLFHCKHVDRQLCGAWQVHQGVRALNTHG 483
           L+    +YKV WPL ++ + +A+ KY  + RFL   K     L    +     R   T+ 
Sbjct: 763 LDMLKFTYKVPWPLELIANTEAIKKYNQVMRFLLKVKRAKFVLDKVRRWMWKGRGSTTNN 822

Query: 484 TAISRSSLLCRSMLKFINSLLHYLTFEVIEPNWHLMYNRLQSANSIDEVIQHHDFF 539
                  L+ + +L F+++   Y+   V    W  +   +  A S+DEVI+ H+ +
Sbjct: 823 R--KHHWLVEQKLLHFVDAFHQYVMDRVYHSAWRELCESMTVAKSLDEVIEAHEAY 876