Miyakogusa Predicted Gene

Lj0g3v0277769.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0277769.1 tr|G7LEC2|G7LEC2_MEDTR Gamma-tubulin complex
component OS=Medicago truncatula GN=MTR_8g011450 PE=4
S,88.69,0,Spc97_Spc98,Spc97/Spc98; GAMMA-TUBULIN COMPLEX COMPONENT 2
(GCP-2),NULL; GAMMA TUBULIN COMPLEX PROTE,CUFF.18456.1
         (654 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G17410.1 | Symbols:  | Spc97 / Spc98 family of spindle pole b...   882   0.0  
AT5G17410.2 | Symbols:  | Spc97 / Spc98 family of spindle pole b...   881   0.0  
AT5G06680.1 | Symbols: SPC98, ATGCP3, ATSPC98, GCP3 | spindle po...   131   1e-30
AT3G53760.1 | Symbols: GCP4, ATGCP4 | GAMMA-TUBULIN COMPLEX PROT...    79   1e-14
AT1G80260.1 | Symbols: emb1427 | Spc97 / Spc98 family of spindle...    61   3e-09
AT1G20570.1 | Symbols:  | Spc97 / Spc98 family of spindle pole b...    60   4e-09

>AT5G17410.1 | Symbols:  | Spc97 / Spc98 family of spindle pole body
           (SBP) component | chr5:5730651-5736415 FORWARD
           LENGTH=678
          Length = 678

 Score =  882 bits (2280), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 441/654 (67%), Positives = 507/654 (77%), Gaps = 23/654 (3%)

Query: 1   MNQSTTGFQNPIGYYDPAVQELIVIDDVLSAMVAVEGRYILIKTVRGKKDDVTFLVDPSM 60
           ++ S++G +  IG YD  VQELIVIDD+LSA+V +EGRYI IK   GK+D + F VDPSM
Sbjct: 44  LDSSSSGVEQAIGCYDTPVQELIVIDDLLSALVGIEGRYISIKRFHGKEDSIAFQVDPSM 103

Query: 61  DLALQELAKRIFPLCRSYLLINQFVESRSQFQNGLVNHAFSAALRALLIDYQAMVAQLEH 120
           DLALQELAKRIFPLC  YLLI+QFVES SQF+NGLVNHAF+AALRALL+DYQAMVAQLEH
Sbjct: 104 DLALQELAKRIFPLCEYYLLIDQFVESSSQFKNGLVNHAFAAALRALLLDYQAMVAQLEH 163

Query: 121 QFRLGRLSLQGLWFYCQPMMRSMQALSTVIQMASVNNFSGSAVLNLLQSQAKAMAGDNAV 180
           QFRLGRLS+QGLWFYCQPMM SM+AL+ VIQ AS   F GS VLNLLQSQAKAMAGDN+V
Sbjct: 164 QFRLGRLSIQGLWFYCQPMMGSMRALAAVIQQASTKQFVGSGVLNLLQSQAKAMAGDNSV 223

Query: 181 RLLLDKMAQCASSAYMSILERWVYEGVIDDPYGEFFIAEDKSLQKDSLTQDYEAKYWRQR 240
           R LL+KM +CAS+AY+SILERWVYEG+IDDPYGEFFIAE++SL+K+SL+QD  AKYW QR
Sbjct: 224 RSLLEKMTECASNAYLSILERWVYEGIIDDPYGEFFIAENRSLKKESLSQDSTAKYWSQR 283

Query: 241 YSLKDGIPSFLANIAGTILTTGKYLNVMRECGHNVQVPPSENSKLMSLGSNHHYLECIKA 300
           YSLKD IP FLANIA TILTTGKYLNVMRECGHNVQVP SE SKL   GSNHHYLECIKA
Sbjct: 284 YSLKDTIPGFLANIAATILTTGKYLNVMRECGHNVQVPISERSKLTIFGSNHHYLECIKA 343

Query: 301 AYNFASGELLNLIKEKYDLTGRLRSIKHYLLLDQGDFLVHFMDIARDELAKKPDEVSVEK 360
           A+ FAS EL+NLIK+KYDL GRLRSIKHYLLLDQGDFLVHFMDIAR+EL KK  E+SVEK
Sbjct: 344 AHEFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVHFMDIAREELNKKVHEISVEK 403

Query: 361 LQSXXXXXXXXXXXXXXPFHEGLTCVVERSSLLKRLGTFNDLEVSQRNSGNNDLLEEPVS 420
           LQS              P HE LTC V+R+SLL  LG   D         +++ +E+P+S
Sbjct: 404 LQSLLDLALRTTAAAADPRHEDLTCCVDRASLLTTLGMHKD--------TDSNSIEDPMS 455

Query: 421 ITCLETFSLSYKVHWPLSIVLSRKALTKYQLIFRFLFHCKHVDRQLCGAWQVHQGVRALN 480
           IT LETFSLSYKV WPLSIV+S+KAL+KYQLIFRFLFHCKHV+RQLCGAWQ+HQG+R++N
Sbjct: 456 ITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCGAWQIHQGIRSMN 515

Query: 481 THGTAISRSSLLCRSMLKFINSLLHYLTFEVIEPNWHLMYNRLQSANSIDEVIQHHDFFX 540
           + GTAI RSSLLCRSMLKFI+SLLHYLTFEV+EPNWH+M++RLQS  S+DEVIQHHDFF 
Sbjct: 516 SKGTAILRSSLLCRSMLKFISSLLHYLTFEVLEPNWHVMHDRLQSTRSVDEVIQHHDFFL 575

Query: 541 XXXXXXXXXXXXXXXXXXXXXXSMCLQYAAATQWLISSSIVLHSPEEPNDDSVRSKQRKS 600
                                 S+CLQYAAATQWLISSSI ++S   P    +R      
Sbjct: 576 DKCLRGCLLLLPDVLKKMEKLKSVCLQYAAATQWLISSSIDINSQSHPQKTMIR------ 629

Query: 601 GQILKSTTNNAAVTDSVLKFEKEFNAEXXXXXXXXXXXXHAEPYLAHLAQWILG 654
                    +  VT+S+  FE+EFN+E             AEPYL HL+QWILG
Sbjct: 630 ---------DTTVTESIFNFEREFNSELQSLGPVLSKGSQAEPYLTHLSQWILG 674


>AT5G17410.2 | Symbols:  | Spc97 / Spc98 family of spindle pole body
           (SBP) component | chr5:5730651-5736415 FORWARD
           LENGTH=679
          Length = 679

 Score =  881 bits (2277), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 441/653 (67%), Positives = 506/653 (77%), Gaps = 23/653 (3%)

Query: 2   NQSTTGFQNPIGYYDPAVQELIVIDDVLSAMVAVEGRYILIKTVRGKKDDVTFLVDPSMD 61
           + S++G +  IG YD  VQELIVIDD+LSA+V +EGRYI IK   GK+D + F VDPSMD
Sbjct: 46  SSSSSGVEQAIGCYDTPVQELIVIDDLLSALVGIEGRYISIKRFHGKEDSIAFQVDPSMD 105

Query: 62  LALQELAKRIFPLCRSYLLINQFVESRSQFQNGLVNHAFSAALRALLIDYQAMVAQLEHQ 121
           LALQELAKRIFPLC  YLLI+QFVES SQF+NGLVNHAF+AALRALL+DYQAMVAQLEHQ
Sbjct: 106 LALQELAKRIFPLCEYYLLIDQFVESSSQFKNGLVNHAFAAALRALLLDYQAMVAQLEHQ 165

Query: 122 FRLGRLSLQGLWFYCQPMMRSMQALSTVIQMASVNNFSGSAVLNLLQSQAKAMAGDNAVR 181
           FRLGRLS+QGLWFYCQPMM SM+AL+ VIQ AS   F GS VLNLLQSQAKAMAGDN+VR
Sbjct: 166 FRLGRLSIQGLWFYCQPMMGSMRALAAVIQQASTKQFVGSGVLNLLQSQAKAMAGDNSVR 225

Query: 182 LLLDKMAQCASSAYMSILERWVYEGVIDDPYGEFFIAEDKSLQKDSLTQDYEAKYWRQRY 241
            LL+KM +CAS+AY+SILERWVYEG+IDDPYGEFFIAE++SL+K+SL+QD  AKYW QRY
Sbjct: 226 SLLEKMTECASNAYLSILERWVYEGIIDDPYGEFFIAENRSLKKESLSQDSTAKYWSQRY 285

Query: 242 SLKDGIPSFLANIAGTILTTGKYLNVMRECGHNVQVPPSENSKLMSLGSNHHYLECIKAA 301
           SLKD IP FLANIA TILTTGKYLNVMRECGHNVQVP SE SKL   GSNHHYLECIKAA
Sbjct: 286 SLKDTIPGFLANIAATILTTGKYLNVMRECGHNVQVPISERSKLTIFGSNHHYLECIKAA 345

Query: 302 YNFASGELLNLIKEKYDLTGRLRSIKHYLLLDQGDFLVHFMDIARDELAKKPDEVSVEKL 361
           + FAS EL+NLIK+KYDL GRLRSIKHYLLLDQGDFLVHFMDIAR+EL KK  E+SVEKL
Sbjct: 346 HEFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVHFMDIAREELNKKVHEISVEKL 405

Query: 362 QSXXXXXXXXXXXXXXPFHEGLTCVVERSSLLKRLGTFNDLEVSQRNSGNNDLLEEPVSI 421
           QS              P HE LTC V+R+SLL  LG   D         +++ +E+P+SI
Sbjct: 406 QSLLDLALRTTAAAADPRHEDLTCCVDRASLLTTLGMHKD--------TDSNSIEDPMSI 457

Query: 422 TCLETFSLSYKVHWPLSIVLSRKALTKYQLIFRFLFHCKHVDRQLCGAWQVHQGVRALNT 481
           T LETFSLSYKV WPLSIV+S+KAL+KYQLIFRFLFHCKHV+RQLCGAWQ+HQG+R++N+
Sbjct: 458 TGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCGAWQIHQGIRSMNS 517

Query: 482 HGTAISRSSLLCRSMLKFINSLLHYLTFEVIEPNWHLMYNRLQSANSIDEVIQHHDFFXX 541
            GTAI RSSLLCRSMLKFI+SLLHYLTFEV+EPNWH+M++RLQS  S+DEVIQHHDFF  
Sbjct: 518 KGTAILRSSLLCRSMLKFISSLLHYLTFEVLEPNWHVMHDRLQSTRSVDEVIQHHDFFLD 577

Query: 542 XXXXXXXXXXXXXXXXXXXXXSMCLQYAAATQWLISSSIVLHSPEEPNDDSVRSKQRKSG 601
                                S+CLQYAAATQWLISSSI ++S   P    +R       
Sbjct: 578 KCLRGCLLLLPDVLKKMEKLKSVCLQYAAATQWLISSSIDINSQSHPQKTMIR------- 630

Query: 602 QILKSTTNNAAVTDSVLKFEKEFNAEXXXXXXXXXXXXHAEPYLAHLAQWILG 654
                   +  VT+S+  FE+EFN+E             AEPYL HL+QWILG
Sbjct: 631 --------DTTVTESIFNFEREFNSELQSLGPVLSKGSQAEPYLTHLSQWILG 675


>AT5G06680.1 | Symbols: SPC98, ATGCP3, ATSPC98, GCP3 | spindle pole
           body component 98 | chr5:2056741-2059369 FORWARD
           LENGTH=838
          Length = 838

 Score =  131 bits (330), Expect = 1e-30,   Method: Compositional matrix adjust.
 Identities = 130/563 (23%), Positives = 238/563 (42%), Gaps = 88/563 (15%)

Query: 19  VQELIVIDDVLSAMVAVEGRYILIKT-VRGKKDDVTFLVDPSMDLALQELAKRIFPLCRS 77
           V E +++ DVL A   ++G+Y+   + + G     +  V  +  + ++ L++  +   + 
Sbjct: 184 VTEEVLVRDVLYASQGIDGKYVKFNSEIDGYAVQESVKVPRATRIMVRMLSELGWLFRKV 243

Query: 78  YLLINQFVESRSQFQNGLVNHAFSAALRALLIDYQAMVAQLEHQ---------------- 121
              I + ++       G V  AF AAL+  L DY  ++A LE Q                
Sbjct: 244 KTFITESMDRFPAEDVGTVGQAFCAALQDELSDYYKLLAVLEAQAMNPIPLVSESASSNN 303

Query: 122 -FRLGRLSLQGLWFYCQPMMRSMQALSTVIQMASVNNFSGSAVLNLLQSQAKAMAGDNAV 180
              L RLS+   WF  +PM++ M+ ++ ++    V    G A+   +   A+   GD  V
Sbjct: 304 YLSLRRLSV---WF-AEPMVK-MRLMAVLVDKCKV--LRGGAMAGAIHLHAQH--GDPLV 354

Query: 181 RLLLDKMAQCASSAYMSILERWVYEGVIDDPYGEFFIAEDKSLQKDSLTQDYEAKYWRQR 240
              +  + +C  S    ++  WV EG ++D +GEFF+   + ++ D L        WR+ 
Sbjct: 355 HDFMMSLLRCVCSPLFEMVRSWVLEGELEDTFGEFFVV-GQPVKVDLL--------WREG 405

Query: 241 YSLKDG-IPSFLA-NIAGTILTTGKYLNVMRECGHN-----------VQVPPSENSKLMS 287
           Y L    +PSF++ ++A  IL TGK +N +R C  +                +     + 
Sbjct: 406 YKLHPAMLPSFISPSLAQRILRTGKSINFLRVCCDDHGWADAASEAAAASGTTTRRGGLG 465

Query: 288 LGSNHHYLECIKAAYNFASGELLNLIKEKYDLTGRLRSIKHYLLLDQGDFLVHFMDIARD 347
            G        +  A       LL+++ ++Y       +IK YLLL QGDF+ + MDI   
Sbjct: 466 YGETDALEHLVTEAAKRIDKHLLDVLYKRYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGP 525

Query: 348 ELAKKPDEVSVEKLQSXXXXXXXXXXXXXXPFHEGLTCVVERSSLLKRLGTFNDLEVSQR 407
           +L++  + +S  +L                 F E    +   ++        + L V   
Sbjct: 526 KLSEPANNISSFELAG---------------FLEA--AIRASNAQYDDRDMLDRLRVKMM 568

Query: 408 NSGNNDLLEEPVSITCLETFSLSYKVHWPLSIVLSRKALTKYQLIFRFLFHCKHVDRQLC 467
             G+ D           + FSL Y+   PL  V +   L+KY  +F FL+  K V+  L 
Sbjct: 569 PHGSGD--------RGWDVFSLEYEARVPLDTVFTESVLSKYLRVFNFLWKLKRVEHALI 620

Query: 468 GAWQVHQGVRALNTHG-------------TAISRSSLLCRSMLKFINSLLHYLTFEVIEP 514
           G W+  +    + ++              +A+ R  +L   M  F+ +  +Y+ FEV+E 
Sbjct: 621 GIWKTMKP-NCITSNSFVKLQSSVKLQLLSALRRCQVLWNEMNHFVTNFQYYIMFEVLEV 679

Query: 515 NWHLMYNRLQSANSIDEVIQHHD 537
           +W      +++A  +D+++  H+
Sbjct: 680 SWSNFSKEMEAAKDLDDLLAAHE 702


>AT3G53760.1 | Symbols: GCP4, ATGCP4 | GAMMA-TUBULIN COMPLEX PROTEIN
           4 | chr3:19918182-19922264 REVERSE LENGTH=745
          Length = 745

 Score = 78.6 bits (192), Expect = 1e-14,   Method: Compositional matrix adjust.
 Identities = 100/486 (20%), Positives = 179/486 (36%), Gaps = 111/486 (22%)

Query: 146 LSTVIQMASVNNFSGSAVLNLLQSQAKAMAGDNAVRLLLDKMAQCASSAYMSILERWVYE 205
           L  VI     ++  G  +LN+L    +   G   +R  L ++         + L  W+  
Sbjct: 164 LYEVILEIERDDIRGGQLLNVLNK--RCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVY 221

Query: 206 GVIDDPYGEFFIA--EDKSLQKDSLTQDYEAKY------------WRQRYSLK-DGIPSF 250
           G++ DP+GEFFI   +D  L   S  ++   K             W   + +  D +P +
Sbjct: 222 GILQDPHGEFFIKRQDDGDLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDY 281

Query: 251 LA-NIAGTILTTGKYLNVMRECGHNVQ--------------------------------- 276
           +   +  +IL  GK + V+R      Q                                 
Sbjct: 282 IPMRLGESILFAGKAIRVLRNPSPAFQFQKDKSFQQTMRGSQRIRGFMHSDFPETETELD 341

Query: 277 --------VPPSENSKLMSL------GSNHHY--LEC-IKAAYNFASGELLNLIKEKYDL 319
                   +P SE  K+ ++       S  H    EC + +    A+  L  L+  + DL
Sbjct: 342 ADLTGGELLPQSEADKIEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADL 401

Query: 320 TGRLRSIKHYLLLDQGDFLVHFMDIARDELAKKPDEVSVEKLQSXXXXXXXXXXXXXXPF 379
            G L+++K Y LL++GDF   F++ +R  +   P + + E                  PF
Sbjct: 402 NGHLKALKDYFLLEKGDFFQCFLEESRQLMRLPPRQSTGES-------------DLMVPF 448

Query: 380 HEGLT-CVVERSSLLKRLG-TFNDLEVSQRNSGNNDLLEEPVSIT------------CLE 425
               T  + E      R+        V+ R+S   D++   VS+T              +
Sbjct: 449 QLAATKTIAEEDKYFSRVSLRMPSFGVTVRSS-QADMVRSKVSLTGKANLTSDTSVDGWD 507

Query: 426 TFSLSYKVHWPLSIVLSRKALTKYQLIFRFLFHCKHVDRQLCGAWQ--VHQG------VR 477
             +L Y V WP+ +  +++ L+KY  +F++L   K    +L  +W   +HQ        R
Sbjct: 508 AIALEYSVDWPMQLFFTQEVLSKYLKVFQYLIRLKRTQMELEKSWASVMHQDHIESAQHR 567

Query: 478 ALNTHGTAISRSSLLCRSMLK-------FINSLLHYLTFEVIEPNWHLMYNRLQSANSID 530
               +G+   +     R M +        I +L  Y+  +VIE  W ++   +  +    
Sbjct: 568 KDGLNGSTSQQRRQGIRPMWRVREHMAFLIRNLQFYIQVDVIESQWKVLQTHIHDSQDFT 627

Query: 531 EVIQHH 536
           E++  H
Sbjct: 628 ELVGFH 633


>AT1G80260.1 | Symbols: emb1427 | Spc97 / Spc98 family of spindle
           pole body (SBP) component | chr1:30175924-30180511
           FORWARD LENGTH=995
          Length = 995

 Score = 60.8 bits (146), Expect = 3e-09,   Method: Compositional matrix adjust.
 Identities = 55/250 (22%), Positives = 99/250 (39%), Gaps = 22/250 (8%)

Query: 298 IKAAYNFASGELLNLIKEKYDLTGRLRSIKHYLLLDQGDFLVHFMDIARDELAKKP---D 354
           I+   ++    +L+ +   + L   L  ++   LL  GD L HF+ +  D L K     D
Sbjct: 633 IRRQVDYIGKVILSKLMNDWKLMHELAVLRAIYLLGSGDLLQHFLTVIFDRLGKGESSND 692

Query: 355 EVSVEKLQSXXXXXXXXXXXXXXPFHEGLTCVVERSSLLKR-LGTFNDLEVSQRNSGNND 413
           +  +  +                P  + L   + R    K   G    L  ++++  N+ 
Sbjct: 693 DFELNIILQESIRNSADAMLLSSP--DSLVVSISREDRDKDDKGDIIPLSSTRKSRVNS- 749

Query: 414 LLEEPVSITCLETFSLSYKVHWPLSIVLSRKALTKYQLIFRFLFHCKH----VDRQLCGA 469
                  I CLE+   +YKV WPL ++ + +A+ KY  +  FL   K     +D+     
Sbjct: 750 -----FGIDCLESLKFTYKVPWPLELIANSEAIKKYNQVMGFLLKVKRAKYVLDKARRWM 804

Query: 470 WQVHQGVRALNTHGTAISRSSLLCRSMLKFINSLLHYLTFEVIEPNWHLMYNRLQSANSI 529
           W+       +  H        LL + +L F+++   Y+   V    W  +   +  A S+
Sbjct: 805 WKGKGSATKIRKH------HWLLEQKLLNFVDAFHQYVMDRVYHTAWRELCEAMVKAGSL 858

Query: 530 DEVIQHHDFF 539
           DEVI  H+ +
Sbjct: 859 DEVIYVHETY 868


>AT1G20570.1 | Symbols:  | Spc97 / Spc98 family of spindle pole body
           (SBP) component | chr1:7122018-7126559 REVERSE
           LENGTH=976
          Length = 976

 Score = 60.5 bits (145), Expect = 4e-09,   Method: Compositional matrix adjust.
 Identities = 53/231 (22%), Positives = 90/231 (38%), Gaps = 24/231 (10%)

Query: 316 KYDLTGRLRSIKHYLLLDQGDFLVHFMDIARDELAKKP---DEVSVEKLQSXXXXXXXXX 372
           ++ L   L  ++   LL  GD L HF+ +  D L K     D+  +  +           
Sbjct: 650 EWKLMHELAVLRAIYLLGSGDLLQHFLTVIFDRLGKGESSNDDFELNIIIQESIRNSADT 709

Query: 373 XXXXXPFHEGLTCVVERSSLLKR----LGTFNDLEVSQRNSGNNDLLEEPVSITCLETFS 428
                P  + L   +     L R     G    L   + +S NN       +I CLE+  
Sbjct: 710 MLLSSP--DALVVSISSEGCLDRDKDDKGDVKSLSSPRESSVNN------YAIDCLESLK 761

Query: 429 LSYKVHWPLSIVLSRKALTKYQLIFRFLFHCKHVDRQLCGAWQVHQGVRALNTHGTAISR 488
            +YKV WPL ++ + +A+ KY  + R  +      R +   W+       +  H      
Sbjct: 762 FTYKVPWPLELIANSEAIKKYNQVKRAKYVLDKARRLM---WKGKGSATKIRKH------ 812

Query: 489 SSLLCRSMLKFINSLLHYLTFEVIEPNWHLMYNRLQSANSIDEVIQHHDFF 539
             LL + +L F+++   Y+   V    W  +   +  A S+DEVI  H+ +
Sbjct: 813 HCLLEQKLLNFVDAFHQYVMDRVYHTAWRELCEAMVKAGSLDEVIDVHETY 863