Miyakogusa Predicted Gene
- Lj6g3v1078670.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj6g3v1078670.1 Non Chatacterized Hit- tr|I3S8H4|I3S8H4_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,99.72,0,Glyco_hydro_18,Glycoside hydrolase, family 18, catalytic
domain; no description,Chitinase II; no des,CUFF.59006.1
(365 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma15g25060.1 495 e-140
Glyma17g08400.1 340 1e-93
Glyma15g25020.1 272 5e-73
Glyma17g33190.1 226 4e-59
Glyma17g11170.1 197 2e-50
Glyma13g22350.1 184 2e-46
Glyma15g41240.1 58 1e-08
>Glyma15g25060.1
Length = 365
Score = 495 bits (1275), Expect = e-140, Method: Compositional matrix adjust.
Identities = 245/339 (72%), Positives = 287/339 (84%), Gaps = 1/339 (0%)
Query: 21 YFSSAEAAIKGGYWYSGSGLSVSDINPSHFTHLFCAFADLDLQTNTLTISPANAAQFSTF 80
+F+ ++AAIKGGYWYS SGL+VS+INPSHFTHLFCAFA LD TN +TIS ++++QFSTF
Sbjct: 21 HFTPSKAAIKGGYWYSESGLAVSNINPSHFTHLFCAFAHLDPNTNKVTISSSDSSQFSTF 80
Query: 81 TQTLQAKNPSVKTLLSIGGGGGHDLAANFSKMASQASSRKTFIDSSIQLARKNNFHGLDL 140
TQTLQAKNPSVKTLLSIGGG G LAANFS+MA QA++RK+FIDSSIQ AR NNF GLDL
Sbjct: 81 TQTLQAKNPSVKTLLSIGGGFGPSLAANFSRMARQANTRKSFIDSSIQQARSNNFLGLDL 140
Query: 141 DWEYPSTNTDKTNFGSLIKEWRAAVARDSSNTGKPXXXXXXXXXXXXQITELQFYPGQDI 200
DWEYPS++TDKTNF SLIKEW+ AV ++S +GK QIT L++YPG+D+
Sbjct: 141 DWEYPSSDTDKTNFASLIKEWKEAVTKESRTSGKAPLFLSAAVAGSDQITPLKYYPGKDV 200
Query: 201 ANNLDWVNVMAYDLFRSDDYPTLTQPPAPLRNPRGQFSADEGVTKWIQ-LGVPQSKLALG 259
ANNLD+VNVMAYDLF S+ YPT+TQPPAP NPRGQFSA++GVT+W + LGVP +KL LG
Sbjct: 201 ANNLDFVNVMAYDLFTSEGYPTVTQPPAPWNNPRGQFSAEQGVTEWNKTLGVPLNKLNLG 260
Query: 260 LPFYGFKWSLSDPNQHRLFAPATKGLGQVKYKDIKNAGAQIVYNSTFVTNYCFKGTDWFG 319
LPFYG+KWSLSD N++ LFAPA +GLG VKYKDIKN AQ+V++ST+VTNYCFKGTDWFG
Sbjct: 261 LPFYGYKWSLSDSNKNGLFAPAKQGLGAVKYKDIKNVAAQVVFDSTYVTNYCFKGTDWFG 320
Query: 320 YDDTQSIAAKVSYAKQKGLFGYFAWQIEQDSNWALSQAG 358
YDDTQSI+AKV AKQKGL GYFAW IEQDSNWALSQA
Sbjct: 321 YDDTQSISAKVVNAKQKGLVGYFAWHIEQDSNWALSQAA 359
>Glyma17g08400.1
Length = 372
Score = 340 bits (872), Expect = 1e-93, Method: Compositional matrix adjust.
Identities = 181/348 (52%), Positives = 221/348 (63%), Gaps = 15/348 (4%)
Query: 24 SAEAAIKGGYWYSGSGLSVSDINPSHFTHLFCAFADLDLQTNTLTISPANAAQFSTFTQT 83
S IKGGYW+ GS +VSDIN + +THLFCAFADL+ T +TIS +NA QFSTFTQT
Sbjct: 24 STAQKIKGGYWFPGSSFAVSDINSTLYTHLFCAFADLNASTYQVTISSSNAPQFSTFTQT 83
Query: 84 LQAKNPSVKTLLSIGGGGGHDLAANFSKMASQASSRKTFIDSSIQLARKNNFHGLDLDWE 143
+Q KNPSVKTLLSIGGG + + FS MASQA RKTFIDSSIQLAR NNFHGLD+DWE
Sbjct: 84 VQKKNPSVKTLLSIGGGASN--PSTFSAMASQAGRRKTFIDSSIQLARSNNFHGLDMDWE 141
Query: 144 YPSTNTDKTNFGSLIKEWRAAVARDSSNTGKPXXXXXXXXXXXXQITELQFYPGQDIANN 203
YPST+TD TN+G LI+EWR A+ ++ N+GK L Y QD+ N
Sbjct: 142 YPSTSTDMTNYGFLIREWRTALVNEARNSGKQILLLVGAVFYSSNYYSLN-YTIQDVINA 200
Query: 204 LDWVNVMAYDLFRSDDYPTLTQPPA---PLRNPRG-QFSADEGVTKWIQLGVPQSKLALG 259
D+VNVMAYD + + YP T PPA L +P + S D G+ WI G+P SKL LG
Sbjct: 201 FDFVNVMAYDFYGPNWYPNFTAPPAALYALNHPAANRVSGDMGIRDWIGSGMPASKLVLG 260
Query: 260 LPFYGFKWSLSDPNQHRLF----APATKGLGQVKYKDIKNAGAQ----IVYNSTFVTNYC 311
+PFYG+ W L + N LF A G G + Y I+ +Q +NST V++YC
Sbjct: 261 VPFYGYAWRLLNSNNRGLFDRANGSAFGGDGSMGYSQIREFVSQNRAACTFNSTVVSDYC 320
Query: 312 FKGTDWFGYDDTQSIAAKVSYAKQKGLFGYFAWQIEQDSNWALSQAGE 359
GT W GYDD QS++AKV+YAK L G+F W + D NW LSQA
Sbjct: 321 SSGTTWIGYDDVQSVSAKVAYAKTNNLGGHFVWHVGADYNWVLSQAAS 368
>Glyma15g25020.1
Length = 331
Score = 272 bits (695), Expect = 5e-73, Method: Compositional matrix adjust.
Identities = 145/335 (43%), Positives = 192/335 (57%), Gaps = 13/335 (3%)
Query: 29 IKGGYWYSGSGLSVSDINPSHFTHLFCAFADLDLQTNTLTISPANAAQFSTFTQTLQAKN 88
++ GYWYSGSG VSDIN + +THL CAFA+L+ T L +SP + FS+FT T++ KN
Sbjct: 1 LQAGYWYSGSGFPVSDINSALYTHLICAFAELNSSTYELYVSPEDEQSFSSFTTTVKQKN 60
Query: 89 PSVKTLLSIGGGGGHDLAANFSKMASQASSRKTFIDSSIQLARKNNFHGLDLDWEYPSTN 148
PS+ TLLSI GG G+D S M S+ SSRK FI SSI++AR F GLDL W P T
Sbjct: 61 PSITTLLSIAGGNGNDTV--LSLMVSKDSSRKYFIQSSIRIARLYGFQGLDLSW-VPETI 117
Query: 149 TDKTNFGSLIKEWRAAVARDSSNTGKPXXXXXXXXXXXXQITELQFYPGQDIANNLDWVN 208
+D N G L +EWRAA +++N + YP + I NNL+WV+
Sbjct: 118 SDMNNMGRLFEEWRAAAKSEAANDSTQVLILTAAVHFRPGLDSAS-YPVESIQNNLNWVH 176
Query: 209 VMAYDLFRSDDYPTLTQPPAPLRNPRGQFSADEGVTKWIQLGVPQSKLALGLPFYGFKWS 268
++ YD + T A L +P + D G+ +WI GV SKL LGLPFYG+ W+
Sbjct: 177 ILTYD-YHMPQLANFTAAHAALYDPSSSVNTDNGIKEWIGSGVTASKLVLGLPFYGYAWN 235
Query: 269 LSDPNQHRLFA----PATKGLGQVKYKDIK----NAGAQIVYNSTFVTNYCFKGTDWFGY 320
L +P + + A PA G + YKDIK G + YN+T+V NY G+ W GY
Sbjct: 236 LRNPEDNAIGASATGPAIGKSGAMNYKDIKAYIQRYGGHVKYNATYVVNYFSNGSTWIGY 295
Query: 321 DDTQSIAAKVSYAKQKGLFGYFAWQIEQDSNWALS 355
DD + + KVSYA++ L GY WQ+ D NW LS
Sbjct: 296 DDVEVVKMKVSYARENKLLGYAVWQVPYDDNWVLS 330
>Glyma17g33190.1
Length = 384
Score = 226 bits (575), Expect = 4e-59, Method: Compositional matrix adjust.
Identities = 129/341 (37%), Positives = 183/341 (53%), Gaps = 16/341 (4%)
Query: 28 AIKGGYWYSGSGLSVSDINPSHFTHLFCAFADLDLQTNTLTISPANAAQFSTFTQTLQAK 87
+++ YW +G LS S I+ +FTH++ AF D Q L+++ + F L+ +
Sbjct: 40 SVRAAYWPAGDDLSPSSIDTKYFTHIYYAFIQQDPQLFHLSVTEFDEKWIPKFINGLRYR 99
Query: 88 NPSVKTLLSIGGGGGHDLAANFSKMASQASSRKTFIDSSIQLARKNNFHGLDLDWEYPST 147
P VKTLLSIGGGG + A FS MAS +R+ FI+S+I +AR+ F+GLDLDWE+P
Sbjct: 100 YPPVKTLLSIGGGGSNSTA--FSLMASNKHTRQVFINSTIHVARQYGFNGLDLDWEFPDD 157
Query: 148 NTDKTNFGSLIKEWRAAVARDSSNTGKPXXXXXXXXXXXXQITEL----QFYPGQDIANN 203
D +N G L +EW A+ ++ + KP I + + YP Q I N
Sbjct: 158 EHDMSNLGVLFQEWHQALVVEAKISRKPRLLLTAAVYYASTIKLIGDGPRSYPAQAIRNY 217
Query: 204 LDWVNVMAYDLFRSDDYPTLTQPPAPLRNPRGQFSADEGVTKWIQLGVPQSKLALGLPFY 263
LDW + M +D + D T A L +P+ S G+ WI+ GVP KL +GLP Y
Sbjct: 218 LDWASPMCFDYHGTWD--NFTGFNAALYDPKSNISTQYGIGSWIESGVPTQKLVMGLPLY 275
Query: 264 GFKWSLSDPNQHRLFAP----ATKGLGQVKYKDI----KNAGAQIVYNSTFVTNYCFKGT 315
G W+L DPN + + A AT G + Y +I K GA +VY+ V Y + GT
Sbjct: 276 GRAWALRDPNVNGVEAEAVGKATDTDGTMDYDEILVFNKENGATVVYDDVAVAFYSYAGT 335
Query: 316 DWFGYDDTQSIAAKVSYAKQKGLFGYFAWQIEQDSNWALSQ 356
W GYDD SI KV +A+ GL GYF W + +D +W +S+
Sbjct: 336 TWIGYDDGPSIKKKVQFARFTGLKGYFFWAVGKDKDWTISR 376
>Glyma17g11170.1
Length = 377
Score = 197 bits (500), Expect = 2e-50, Method: Compositional matrix adjust.
Identities = 131/348 (37%), Positives = 186/348 (53%), Gaps = 24/348 (6%)
Query: 23 SSAEAAIKGGYWYSGSGLSVSDINPSHFTHLFCAFADLDLQTNTLTISPANAAQFSTFTQ 82
+S+ +K YW S IN S FTH++ AF + T L +S + A + FT
Sbjct: 21 TSSTNDVKAIYWLEQPLFPPSAINTSLFTHVYYAFLAPNNVTYKLHVSNSTATNLAKFTT 80
Query: 83 TLQAKNPSVKTLLSIGGGGGHDLAANFSKMASQASSRKTFIDSSIQLARKNNFHGLDLDW 142
TL + +V TL+SIGG + A FS +A+ +++R TFI+S+I LAR FHG+DLDW
Sbjct: 81 TLGS---TVATLISIGGANSN--ATLFSLIAANSAARATFINSTITLARTFGFHGVDLDW 135
Query: 143 EYPSTNTDKTNFGSLIKEWRAAVARDSSNTGKPXXXXXXXXXXXX-----QITELQFYPG 197
E+P T ++ N G L K+WRAA+A +++ TG+ + + L+ YP
Sbjct: 136 EFPRTASEMKNLGLLFKKWRAAIAAEAAATGREPLLLTAAVYFSVDYFLSETSSLR-YPV 194
Query: 198 QDIANNLDWVNVMAYDLFRSDDYPTLTQPPAPLRNPRGQFSADEGVTKWIQLGVPQSKLA 257
I NLDWVNVM+YDL + + T PPA L NP+ S G+ WI+ GV K+
Sbjct: 195 GSINENLDWVNVMSYDL--NGPWSNHTGPPAGLFNPKSNASVGYGLGSWIRGGVIPKKVV 252
Query: 258 LGLPFYGFKWSLSDPNQHRLFAP---------ATKGLGQVKYKDIKNAGAQIVYNSTFVT 308
+GLP YG W L D + H + AP L QV + K GA++VY+ +
Sbjct: 253 MGLPLYGRTWQLRDRSVHGIGAPTIGPVPGSDGVMALFQVLQFN-KETGAKVVYDRETAS 311
Query: 309 NYCFKGTDWFGYDDTQSIAAKVSYAKQKGLFGYFAWQIEQD-SNWALS 355
Y + G+ W GYDD S+A KV +A+ L GYF W D S+W +S
Sbjct: 312 VYSYSGSYWIGYDDPISVAVKVGFAQAFSLRGYFFWAAGLDTSDWKIS 359
>Glyma13g22350.1
Length = 379
Score = 184 bits (466), Expect = 2e-46, Method: Compositional matrix adjust.
Identities = 126/342 (36%), Positives = 183/342 (53%), Gaps = 23/342 (6%)
Query: 29 IKGGYWYSGSGLSVSDINPSHFTHLFCAFADLDLQTNTL-TISPANAAQFSTFTQTLQAK 87
IK YW S IN S FTH++ AF + T L +S + +TFT TL++
Sbjct: 28 IKAIYWLEQPLFPPSAINTSLFTHVYYAFLAPNNVTYELDVVSNSTGTNLATFTNTLRSN 87
Query: 88 NPSVKTLLSIGGGGGHDLAANFSKMASQASSRKTFIDSSIQLARKNNFHGLDLDWEYPST 147
V TL+SIGG + + FS +A+ A++R TFI+S+I +AR F+G+DLDWE+P T
Sbjct: 88 ---VATLISIGGANSN--STLFSLIAANAAARATFINSTITVARTFGFNGIDLDWEFPRT 142
Query: 148 NTDKTNFGSLIKEWRAAVARDSSNTGKPXXXXXXXXXXXX-----QITELQFYPGQDIAN 202
+ + G L KEWRAAV+ +++ TG+ + T L+ YP I
Sbjct: 143 VNEMNDLGLLFKEWRAAVSAEAAATGREPLLLTAAVYFSVDYFLSETTSLR-YPVDSINE 201
Query: 203 NLDWVNVMAYDLFRSDDYPTLTQPPAPLRNPRGQFSADEGVTKWIQLGVPQSKLALGLPF 262
NLDWVNVM+YDL + + T PPA L +P+ S G+ WI+ GV K+ +GLP
Sbjct: 202 NLDWVNVMSYDL--NGPWSNHTGPPAGLFDPKNNASVSYGLGSWIRGGVIPKKVVMGLPL 259
Query: 263 YGFKWSLSDPNQHRLFAPAT-------KGLGQVKYKDIKN-AGAQIVYNSTFVTNYCFKG 314
YG W L +PN H + APA+ + + + N GA +V + + Y + G
Sbjct: 260 YGRTWQLLEPNVHGIGAPASGPGPGSNGAMALFQVLEFNNETGANVVCDKETASVYSYSG 319
Query: 315 TDWFGYDDTQSIAAKVSYAKQKGLFGYFAWQIEQD-SNWALS 355
+ W GYDD +++A KV +A+ L GYF W D S+W +S
Sbjct: 320 SYWVGYDDPKTVAVKVGFAQALSLHGYFFWAAGLDTSDWKIS 361
>Glyma15g41240.1
Length = 87
Score = 58.2 bits (139), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 29/62 (46%), Positives = 37/62 (59%)
Query: 113 ASQASSRKTFIDSSIQLARKNNFHGLDLDWEYPSTNTDKTNFGSLIKEWRAAVARDSSNT 172
+SQ R TFI+S+I L R FH +DLDWE+P T N G L KEWR A+A +
Sbjct: 2 SSQNPQRATFINSTITLPRTFGFHDIDLDWEFPRTANKMKNLGLLFKEWRIAIAAEVIAI 61
Query: 173 GK 174
G+
Sbjct: 62 GR 63