Miyakogusa Predicted Gene
- Lj6g3v0486200.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj6g3v0486200.1 Non Chatacterized Hit- tr|I1JJJ2|I1JJJ2_SOYBN
Uncharacterized protein OS=Glycine max PE=4
SV=1,74.93,0,EF_HAND_1,EF-Hand 1, calcium-binding site; PROBABLE
CALCIUM-ACTIVATED OUTWARD-RECTIFYING POTASSIUM
C,NODE_84114_length_1891_cov_24.810154.path1.1
(350 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma02g46930.1 473 e-133
Glyma14g01790.1 471 e-133
Glyma17g01080.1 311 6e-85
Glyma08g08710.1 204 1e-52
Glyma19g40890.1 185 7e-47
Glyma03g38280.1 184 1e-46
Glyma05g25750.1 180 2e-45
Glyma02g45510.1 179 5e-45
Glyma14g03260.1 139 6e-33
>Glyma02g46930.1
Length = 349
Score = 473 bits (1218), Expect = e-133, Method: Compositional matrix adjust.
Identities = 235/350 (67%), Positives = 274/350 (78%), Gaps = 1/350 (0%)
Query: 1 MASNGAKEPLLSWSPDPAAQKVTXXXXXXXXXXXXTAAPFADLAPPETNGTGSIQLSKSI 60
MA+NGAK+PLLS S D A QK +AP A+L P ETN SI + SI
Sbjct: 1 MANNGAKDPLLSGSLD-ATQKTKQQLLNKRRLLRSRSAPHAELVPTETNCNESIPRTASI 59
Query: 61 FGKLSPSFRQVAIYFAVYIGVGAVIFYLVRNQIKGHKTDGVLDALYFTIVTMTTVGYGDL 120
F L PSF+++AIY AVY+GVGA+IFYLVRNQIKG KTDG+LDALYFTIVTMTTVGYGDL
Sbjct: 60 FQNLHPSFKRMAIYLAVYLGVGALIFYLVRNQIKGQKTDGILDALYFTIVTMTTVGYGDL 119
Query: 121 VPNSTLTKLLACAFVFTGMSLVGLILTKAGDYLVEKQEALLLKAMTIGQKVGPTEILKEV 180
VPNS L KLLACAFVF+GM+L+GLI++KA DYLVEKQE LL+KAM + QK+G TEIL+EV
Sbjct: 120 VPNSHLAKLLACAFVFSGMALIGLIVSKAADYLVEKQELLLVKAMRMHQKIGSTEILREV 179
Query: 181 KNHKIRLKXXXXXXXXXXXIAIGTAFLVSVEKLDVIDAFYCVCSTFTTLGYGDRSFSTQT 240
+ +K R K I +GT FLV+VEKLDVIDAFYCVCST TTLGYGD+SFSTQ
Sbjct: 180 ETNKTRYKLFLVFSLLLILIIVGTIFLVTVEKLDVIDAFYCVCSTITTLGYGDQSFSTQA 239
Query: 241 GRIFAVFWILAGTICLAQLFLCIAEMNTESKQKAIVKWVLTRKMTNXXXXXXXXXXXGSV 300
GRIFAVFWIL GTI LAQLF+ IAE+NTE +QK +VKWVLTRK+TN G+V
Sbjct: 240 GRIFAVFWILTGTITLAQLFVYIAELNTEIRQKELVKWVLTRKVTNLDLEAADLDEDGTV 299
Query: 301 GAAEFIIYKLKEMGKISQEDISVILQEFEELDVDQSGSLSISDLTLSQSS 350
GAAEF+IYKLKEMGKISQEDIS+++QEFE+LDVD SG+LS SD+TL+QSS
Sbjct: 300 GAAEFVIYKLKEMGKISQEDISLVMQEFEQLDVDDSGTLSTSDITLAQSS 349
>Glyma14g01790.1
Length = 348
Score = 471 bits (1211), Expect = e-133, Method: Compositional matrix adjust.
Identities = 238/350 (68%), Positives = 272/350 (77%), Gaps = 2/350 (0%)
Query: 1 MASNGAKEPLLSWSPDPAAQKVTXXXXXXXXXXXXTAAPFADLAPPETNGTGSIQLSKSI 60
MA+NG KEPLL W A QK T +AP A++ P ETNG SI S SI
Sbjct: 1 MANNGVKEPLL-WVSLDATQK-TKQQLNKRSLLRFRSAPHAEIVPTETNGNESIPHSASI 58
Query: 61 FGKLSPSFRQVAIYFAVYIGVGAVIFYLVRNQIKGHKTDGVLDALYFTIVTMTTVGYGDL 120
F L PSF++VAIY AVY+GVGA+IFYLVRNQIKG KTDG+LDALYFTIVTMTTVGYGDL
Sbjct: 59 FQNLHPSFKRVAIYLAVYLGVGALIFYLVRNQIKGQKTDGILDALYFTIVTMTTVGYGDL 118
Query: 121 VPNSTLTKLLACAFVFTGMSLVGLILTKAGDYLVEKQEALLLKAMTIGQKVGPTEILKEV 180
VPNS LTKLLACAFVF+GM+L+GLI++KA DYLVEKQE LL+KAM + QKVG TEIL+EV
Sbjct: 119 VPNSHLTKLLACAFVFSGMALIGLIVSKAADYLVEKQELLLVKAMRMHQKVGSTEILREV 178
Query: 181 KNHKIRLKXXXXXXXXXXXIAIGTAFLVSVEKLDVIDAFYCVCSTFTTLGYGDRSFSTQT 240
+ +K R K I GT FLV+VEKLDVIDAFYCVCST TTLGYGD+SFSTQ
Sbjct: 179 QTNKTRYKLFLVFFLLLILIIAGTIFLVTVEKLDVIDAFYCVCSTITTLGYGDQSFSTQA 238
Query: 241 GRIFAVFWILAGTICLAQLFLCIAEMNTESKQKAIVKWVLTRKMTNXXXXXXXXXXXGSV 300
GRIFAVFWIL GTI LAQLFL IAE+NTE +QK +VKWVLTRK+TN G+V
Sbjct: 239 GRIFAVFWILTGTITLAQLFLYIAELNTEIRQKELVKWVLTRKVTNSDLEAADLDVDGTV 298
Query: 301 GAAEFIIYKLKEMGKISQEDISVILQEFEELDVDQSGSLSISDLTLSQSS 350
AAEF+IYKLKEMGKISQEDIS+++QEFE+LDVD SG+LS SD+TL+QSS
Sbjct: 299 RAAEFVIYKLKEMGKISQEDISLVMQEFEQLDVDDSGTLSTSDITLAQSS 348
>Glyma17g01080.1
Length = 352
Score = 311 bits (797), Expect = 6e-85, Method: Compositional matrix adjust.
Identities = 156/283 (55%), Positives = 199/283 (70%)
Query: 68 FRQVAIYFAVYIGVGAVIFYLVRNQIKGHKTDGVLDALYFTIVTMTTVGYGDLVPNSTLT 127
F+ V ++ A Y+G G + F L +QIKG KT+G LDA+YF +VTMTTVGYGDLVP+S L
Sbjct: 68 FKTVLLWLAAYLGGGTLCFLLTSHQIKGIKTNGFLDAIYFCVVTMTTVGYGDLVPDSQLA 127
Query: 128 KLLACAFVFTGMSLVGLILTKAGDYLVEKQEALLLKAMTIGQKVGPTEILKEVKNHKIRL 187
KLLAC +VFTGM+LVGLIL+KA DY+VEKQE L++ + G+ GP E+ KEV+ +K +
Sbjct: 128 KLLACIYVFTGMALVGLILSKAADYIVEKQEIFLVRTLFKGENFGPEELSKEVETNKAKY 187
Query: 188 KXXXXXXXXXXXIAIGTAFLVSVEKLDVIDAFYCVCSTFTTLGYGDRSFSTQTGRIFAVF 247
K + GT FL +E LD +DA YCVCST TTLGYGD+SFST GR FAVF
Sbjct: 188 KFILAASVFLVLMISGTIFLHYIENLDFVDALYCVCSTVTTLGYGDKSFSTTIGRAFAVF 247
Query: 248 WILAGTICLAQLFLCIAEMNTESKQKAIVKWVLTRKMTNXXXXXXXXXXXGSVGAAEFII 307
WIL+ TICLAQ F +AE TE +QKA+ K VLTRK++ V A EF++
Sbjct: 248 WILSSTICLAQSFAYLAEFYTEERQKAMAKIVLTRKLSLLDLEAADLDGDHVVSATEFVL 307
Query: 308 YKLKEMGKISQEDISVILQEFEELDVDQSGSLSISDLTLSQSS 350
YKLKEMGKI+QEDI V++ F +LDVD+SG+L+ +DL S+SS
Sbjct: 308 YKLKEMGKINQEDILVVMDIFRKLDVDKSGTLTEADLKYSESS 350
>Glyma08g08710.1
Length = 396
Score = 204 bits (518), Expect = 1e-52, Method: Compositional matrix adjust.
Identities = 105/279 (37%), Positives = 161/279 (57%), Gaps = 3/279 (1%)
Query: 69 RQVAIYFAVYIGVGAVIFYLVRNQIKGHKTDGVLDALYFTIVTMTTVGYGDLVPNSTLTK 128
RQ I A+Y+ +G VI++ R+ +T V+DALYF IVTM T+GYGD+ PNST TK
Sbjct: 113 RQGVILLALYLALGVVIYWFNRHNFTATETHPVVDALYFCIVTMCTIGYGDITPNSTATK 172
Query: 129 LLACAFVFTGMSLVGLILTKAGDYLVEKQEALLLKAM--TIGQKVGPTEILKEVKNH-KI 185
L + FV G + ++L+ Y+++ QE +L A+ G+K G + I+ K +I
Sbjct: 173 LFSILFVLVGFGFIDILLSGMVSYVLDLQENHMLTAVKGRRGEKDGKSYIIDVKKGRMRI 232
Query: 186 RLKXXXXXXXXXXXIAIGTAFLVSVEKLDVIDAFYCVCSTFTTLGYGDRSFSTQTGRIFA 245
RLK +G + VEKL +D+FY + TT+GYGD +F T GRIFA
Sbjct: 233 RLKVALALGVVVICTGVGVGVMHFVEKLGWLDSFYLSVMSVTTVGYGDHAFKTMHGRIFA 292
Query: 246 VFWILAGTICLAQLFLCIAEMNTESKQKAIVKWVLTRKMTNXXXXXXXXXXXGSVGAAEF 305
W+L T+ +A+ FL +AE + + + + KW+L + MT G V +E+
Sbjct: 293 AIWLLVSTLAVARAFLYLAEARVDKRHRRMAKWILGQDMTVSEFLAADIDNNGFVSKSEY 352
Query: 306 IIYKLKEMGKISQEDISVILQEFEELDVDQSGSLSISDL 344
+IYKLKEMGK+S++DI + ++F+ LD G ++++DL
Sbjct: 353 VIYKLKEMGKVSEKDIMQVSEKFDRLDAGNCGKITLADL 391
>Glyma19g40890.1
Length = 385
Score = 185 bits (469), Expect = 7e-47, Method: Compositional matrix adjust.
Identities = 100/282 (35%), Positives = 159/282 (56%), Gaps = 6/282 (2%)
Query: 69 RQVAIYFAVYIGVGAVIFYLVRNQIKGHKTDGVLDALYFTIVTMTTVGYGDLVPNSTLTK 128
RQ AVY+ +G VI+ R++ G +T V+DALYF IVTM T+GYGD+ P + TK
Sbjct: 99 RQGMWLLAVYLSIGVVIYSFNRDRFSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPFTK 158
Query: 129 LLACAFVFTGMSLVGLILTKAGDYLVEKQEALLLKAMTIG--QKVG---PTEILKEVKNH 183
+ ACAFV G + ++L+ +++++ QE ++L + +G ++ G I+ K
Sbjct: 159 IFACAFVLVGFGFIDILLSGLVNFVLDLQENMILTGLQMGASEREGFSARNYIVDVAKGR 218
Query: 184 -KIRLKXXXXXXXXXXXIAIGTAFLVSVEKLDVIDAFYCVCSTFTTLGYGDRSFSTQTGR 242
+IRLK I IG+ L VE LD +D+ Y + TT+GYGDR+F T GR
Sbjct: 219 MRIRLKVGLALGVVVMCIGIGSLVLYFVEGLDWVDSIYLSVMSVTTVGYGDRAFKTLPGR 278
Query: 243 IFAVFWILAGTICLAQLFLCIAEMNTESKQKAIVKWVLTRKMTNXXXXXXXXXXXGSVGA 302
+FA W+L T+ +A+ FL +AE + + + + K VL R++T G +
Sbjct: 279 LFAAIWLLFSTLMVARAFLYLAEARIDRRHRRMAKKVLHREITVQDLLAADINNTGFISK 338
Query: 303 AEFIIYKLKEMGKISQEDISVILQEFEELDVDQSGSLSISDL 344
+E++I+ LKEMGKI ++D+ I +F +LD G +++ L
Sbjct: 339 SEYVIFMLKEMGKIQEKDVLQICDQFRKLDPSNCGKITLPHL 380
>Glyma03g38280.1
Length = 376
Score = 184 bits (468), Expect = 1e-46, Method: Compositional matrix adjust.
Identities = 100/283 (35%), Positives = 159/283 (56%), Gaps = 7/283 (2%)
Query: 69 RQVAIYFAVYIGVGAVIFYLVRNQIKGHKTDGVLDALYFTIVTMTTVGYGDLVPNSTLTK 128
RQ A+Y+ +G I+ R++ G +T V+DALYF IVTM T+GYGD+ P + TK
Sbjct: 89 RQGIWLLAMYLSIGVAIYSFNRDRFSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPFTK 148
Query: 129 LLACAFVFTGMSLVGLILTKAGDYLVEKQEALLLKAMTIG---QKVG---PTEILKEVKN 182
+ ACAFV G + ++L+ +++++ QE ++L + +G Q+ G I+ K
Sbjct: 149 IFACAFVLVGFGFIDILLSGLVNFVLDLQENMILTGLQMGASDQREGFSARNYIVDVAKG 208
Query: 183 H-KIRLKXXXXXXXXXXXIAIGTAFLVSVEKLDVIDAFYCVCSTFTTLGYGDRSFSTQTG 241
+IRLK I IG L VE LD +D+ Y + TT+GYGDR+F T G
Sbjct: 209 RMRIRLKVGLALGVVVLCIGIGGLVLYFVEGLDWVDSIYLSVMSVTTVGYGDRAFKTLPG 268
Query: 242 RIFAVFWILAGTICLAQLFLCIAEMNTESKQKAIVKWVLTRKMTNXXXXXXXXXXXGSVG 301
R+FA W+L T+ +A+ FL +AE + + + + K VL R++T G +
Sbjct: 269 RLFAAIWLLFSTLMVARAFLYLAEARIDRRHRRMAKKVLHREITVEDLLAADINNTGFIS 328
Query: 302 AAEFIIYKLKEMGKISQEDISVILQEFEELDVDQSGSLSISDL 344
+E++I+KLKEMGKI ++D+ I +F +LD G +++ +L
Sbjct: 329 KSEYVIFKLKEMGKIQEKDVLQICDQFRKLDPSNCGKITLPNL 371
>Glyma05g25750.1
Length = 382
Score = 180 bits (456), Expect = 2e-45, Method: Compositional matrix adjust.
Identities = 96/277 (34%), Positives = 154/277 (55%), Gaps = 15/277 (5%)
Query: 70 QVAIYFAVYIGVGAVIFYLVRNQIKGHKTDGVLDALYFTIVTMTTVGYGDLVPNSTLTKL 129
Q I A+Y+ +G VI++ R+ +T ++DALYF IVTM T+GYGD+ PNST TKL
Sbjct: 114 QGVILLALYLALGVVIYWFNRHNFTATETHPIVDALYFCIVTMCTIGYGDITPNSTATKL 173
Query: 130 LACAFVFTGMSLVGLILTKAGDYLVEKQEALLLKAM--TIGQKVGPTEILKEVKNHKIRL 187
+ FV G + ++L+ Y+++ QE +L A+ G+K G + I+ +VK ++R+
Sbjct: 174 FSILFVLVGFGFIDILLSGMVSYVLDLQENHMLTAVKGRRGEKDGKSYII-DVKKGRMRI 232
Query: 188 KXXXXXXXXXXXIAIGTAFLVSVEKLDVIDAFYCVCSTFTTLGYGDRSFSTQTGRIFAVF 247
+ + LD F + TT+GYGD +F T GRIFA
Sbjct: 233 RLKVW------------GLCILWRSLDGWIRFIFSVMSVTTVGYGDHAFKTMHGRIFAAI 280
Query: 248 WILAGTICLAQLFLCIAEMNTESKQKAIVKWVLTRKMTNXXXXXXXXXXXGSVGAAEFII 307
W+L T+ +A+ FL +AE + + + + KW+L + MT G V +E++I
Sbjct: 281 WLLVSTLAVARAFLYLAEARVDKRHRRMAKWILGQDMTVSEFLAADIDNNGFVSKSEYVI 340
Query: 308 YKLKEMGKISQEDISVILQEFEELDVDQSGSLSISDL 344
YKLKEMGK+S++DI + ++F+ LD G ++++DL
Sbjct: 341 YKLKEMGKVSEKDIMQVSEKFDRLDAGNCGKITLADL 377
>Glyma02g45510.1
Length = 329
Score = 179 bits (453), Expect = 5e-45, Method: Compositional matrix adjust.
Identities = 96/271 (35%), Positives = 155/271 (57%), Gaps = 4/271 (1%)
Query: 77 VYIGVGAVIFYLVRNQIKGHKTDGVLDALYFTIVTMTTVGYGDLVPNSTLTKLLACAFVF 136
+Y+ +G + Y+ +G T +DA+YFT+VT+ T+GYGD+VP+ST TK+ C F+
Sbjct: 56 LYVAIGVTV-YMTSGSFRGTTTFRPVDAVYFTMVTLCTIGYGDIVPDSTFTKIFTCGFIL 114
Query: 137 TGMSLVGLILTKAGDYLVEKQEALLLKAMTIGQ--KVGPTEILKEVKNH-KIRLKXXXXX 193
G +G +L Y+ + QEA LL M + K+ T ++ E K +IR K
Sbjct: 115 VGFGFLGFLLNGLVAYICDTQEAFLLSMMDENRYKKILRTYMVDEEKGRMRIRTKVCLAL 174
Query: 194 XXXXXXIAIGTAFLVSVEKLDVIDAFYCVCSTFTTLGYGDRSFSTQTGRIFAVFWILAGT 253
IAIGT + VE L+ D+ Y ++ TT+GYGD S T TGR FA+ W+L T
Sbjct: 175 AVVIGCIAIGTVTVHLVEDLNWDDSIYLSITSVTTVGYGDFSLRTVTGRCFAIIWLLVST 234
Query: 254 ICLAQLFLCIAEMNTESKQKAIVKWVLTRKMTNXXXXXXXXXXXGSVGAAEFIIYKLKEM 313
+ +A+ F+ + E + + + + +WVL +K+T GS+ ++F+IYKLK+M
Sbjct: 235 LAVARAFIYLTEYSIHKRNRKMAQWVLQKKITLSDLAAADLDNDGSISKSDFVIYKLKQM 294
Query: 314 GKISQEDISVILQEFEELDVDQSGSLSISDL 344
GKI++ DI I ++F+ L+ G ++++DL
Sbjct: 295 GKITEIDILQISKQFDSLEHGMYGKITLADL 325
>Glyma14g03260.1
Length = 321
Score = 139 bits (349), Expect = 6e-33, Method: Compositional matrix adjust.
Identities = 96/315 (30%), Positives = 150/315 (47%), Gaps = 33/315 (10%)
Query: 36 TAAPFADLAPPETNGTGSIQLSKSIFGKLSPSFRQVAIYFAVYIGVGAVIFYLVRNQIKG 95
T F + + P + T ++LS F V +Y A V Y+ +G
Sbjct: 19 TGVDFQEPSEPHKSSTSIVKLS----------FLGVFLYVAT-----GVTVYMTSGSFRG 63
Query: 96 HKTDGVLDALYFTIVTMTTVGYGDLVPNSTLTKLLACAFVFTGMSLVGLILTKAGDYLVE 155
T +DA+YFT+VT+ +GY D+VP+ST TK+ CAF+ G +G +L Y+ +
Sbjct: 64 STTFRPVDAVYFTMVTLCNIGYVDIVPDSTFTKIFTCAFILVGFGFLGFLLNGLVAYICD 123
Query: 156 KQEALLLKAMTIGQ--KVGPTEILKEVKNH-KIRLKXXXXXXXXXXXIAIGTAFLVSVEK 212
QEA LL + + K+ T ++ E K +IR K IAIGT + VE
Sbjct: 124 IQEAFLLSMVDENRYKKILRTYMVDEEKGRMRIRTKFCLALAVVIDCIAIGTVTVHLVED 183
Query: 213 LDVIDAFYCVCSTFTTLGYGDRSFSTQTGRIFAVFWILAGTICLAQLFLCIAEMNTESKQ 272
L+ D+ Y ++ TT+GYGD S T TGR FA+ W+L T +A+ + + E + + +
Sbjct: 184 LNWDDSIYLSITSVTTVGYGDFSLRTVTGRCFAIIWLLVSTPAVARASIYLTEYSIQKRN 243
Query: 273 KAIVKWVLTRKMTNXXXXXXXXXXXGSVGAAEFIIY---------------KLKEMGKIS 317
+ +WVL +K+T GS+ I Y K +MGKI+
Sbjct: 244 CKMAQWVLQKKITLSDLAAADLDNDGSIRQRSKIDYLVEPIGLNLHAEVTQKFSQMGKIT 303
Query: 318 QEDISVILQEFEELD 332
+ DI I ++F+ L+
Sbjct: 304 EIDILQISKQFDSLE 318