Miyakogusa Predicted Gene

Lj5g3v0962390.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v0962390.1 tr|G7I2L4|G7I2L4_MEDTR Non-lysosomal
glucosylceramidase OS=Medicago truncatula GN=MTR_1g072720 PE=4
,85.21,0,Six-hairpin glycosidases,Six-hairpin glycosidase-like;
seg,NULL; no description,Six-hairpin glycosid,CUFF.54383.1
         (984 letters)

Database: Glyma1.pep 
           75,778 sequences; 25,431,882 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Glyma10g33610.1                                                      1102   0.0  
Glyma07g40310.1                                                       828   0.0  
Glyma20g33990.1                                                       795   0.0  

>Glyma10g33610.1 
          Length = 1031

 Score = 1102 bits (2849), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 543/698 (77%), Positives = 585/698 (83%), Gaps = 44/698 (6%)

Query: 287  YHKTGKCNPPVTFAIAACETQNVSVSVLPSFGLSEGSSVTAKGMWSKMVKDGHFDRENFX 346
            + KT K NPPVTFAIAACETQNV+VSVLPSFGLSE SS+TAK MWSKMVKDG FD+ENF 
Sbjct: 378  WRKTAKGNPPVTFAIAACETQNVNVSVLPSFGLSEESSMTAKHMWSKMVKDGQFDQENFN 437

Query: 347  XXXXXXXXXXETLCAAVSASTWVEPHGKCTVAFSLAWTSPKVKFVKGSSFQRRYTKFYGT 406
                      ETLCAAV+ASTWVEPHGKCTVAFSLAW+SPKVKFVKGS+F RRYTKFYGT
Sbjct: 438  SGPSMPSSPGETLCAAVAASTWVEPHGKCTVAFSLAWSSPKVKFVKGSTFNRRYTKFYGT 497

Query: 407  SDRAAVDLAHDALTHYKRWEEEIEKWQGPVLKDETIPEWYKFTLFNELYFLVAGGTIWID 466
            S++AA DLAHDALTHY RWEEEIEKWQ PVLKDE +PEWYKFTLFNELYFLVAGGTIWID
Sbjct: 498  SEKAAADLAHDALTHYNRWEEEIEKWQNPVLKDEALPEWYKFTLFNELYFLVAGGTIWID 557

Query: 467  SPLQSPLVRSDRDQVNALQNAVVKVTEAKVECRTREVVECXXXXXXXXXXXRGHNHVYEN 526
            SP+ S  +R+D+D+V  L++AVVK TE K+  R R VVE            R    +YE 
Sbjct: 558  SPVLSSNMRNDQDRVRELESAVVKETEDKMSDRKRTVVE---------RHDRADEKLYE- 607

Query: 527  HFVDESHENESVDTLRRENSIGTHNTSTMMGQQYDDNDVGRFLYLEGVEYIMWCTYDVHF 586
                                              DD+DVGRFLYLEGVEYIMWCTYDVHF
Sbjct: 608  ----------------------------------DDDDVGRFLYLEGVEYIMWCTYDVHF 633

Query: 587  YASFALLALFPRIELNIQREFAKAVLCEDGRRVKFLAEGNWGIRKVYGAVPHDLGTHDPW 646
            YASFALL LFPRIELNIQR+FA+AVLCEDGR+VKFLAEGNWGIRKVYGAVPHDLGTHDPW
Sbjct: 634  YASFALLELFPRIELNIQRDFARAVLCEDGRKVKFLAEGNWGIRKVYGAVPHDLGTHDPW 693

Query: 647  FEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDLQFGVDVWPAVRAAMEYMDQFDRDGD 706
             EMNAYNIHDTSKWKDLNPKFVLQVYRDFA TGDLQFGVDVWPAVRAAMEYM+QFDRDGD
Sbjct: 694  HEMNAYNIHDTSKWKDLNPKFVLQVYRDFATTGDLQFGVDVWPAVRAAMEYMEQFDRDGD 753

Query: 707  CLIENDGFPDQTYDTWTVHGVSAYCGCXXXXXXXXXXXXXXXXGDRDFAESCKRKFMKAK 766
             LIENDGFPDQTYDTWTVHGVS YCGC                GDR+FAE+CKRKF+KAK
Sbjct: 754  GLIENDGFPDQTYDTWTVHGVSTYCGCLWLAALQAAAVMALELGDREFAETCKRKFLKAK 813

Query: 767  PVFEEKLWNGSYFNYDSGSSGNSKSIQADQLAGQWYTASSGLPSLFDEPKIKSALRKVYD 826
            P FEEKLWNG+YFNYDSGSSGNSKSIQADQLAGQWYTASSGLPSLF++ KIKSALRKVYD
Sbjct: 814  PAFEEKLWNGTYFNYDSGSSGNSKSIQADQLAGQWYTASSGLPSLFEDSKIKSALRKVYD 873

Query: 827  FNVMKIKGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILSGMEEEAFRTAEG 886
            FNVMK+KGGRMGAVNGMHPNGKVDETCMQSRE+WTGVTYG+AATMI +GMEEEAF TAEG
Sbjct: 874  FNVMKVKGGRMGAVNGMHPNGKVDETCMQSREVWTGVTYGLAATMIHAGMEEEAFTTAEG 933

Query: 887  IFLAGWSEDGYGYWFQTPEAFTIDGHYRSLIYMRPLAIWGMQYALTLPKAILEAPKMNIM 946
            IFLAGWSEDGYGYWFQTPEA+T+DGHYRSL+YMRPLAIWGMQYA+  PKAILEAPK+NIM
Sbjct: 934  IFLAGWSEDGYGYWFQTPEAWTMDGHYRSLMYMRPLAIWGMQYAINRPKAILEAPKINIM 993

Query: 947  DRIHISPLNGGFSHNETGVRKIATKAKCFSNSVFNCAC 984
            DRIH+SP+ GG+SHNETGVRKIATKA CFSNSVFNCAC
Sbjct: 994  DRIHLSPVIGGYSHNETGVRKIATKAGCFSNSVFNCAC 1031



 Score =  521 bits (1343), Expect = e-147,   Method: Compositional matrix adjust.
 Identities = 260/293 (88%), Positives = 273/293 (93%), Gaps = 8/293 (2%)

Query: 1   MVSGSIFHFRKNSWPAEEYISKSTLQRFDYDSAAPPEQAWRRKLNSHANLLKEFKITFME 60
           MVSG+IFH RKNSWP +EYISKSTLQ FDYDS+APPEQAWRR+LNSHANLLKEF++TF E
Sbjct: 1   MVSGNIFHCRKNSWPPQEYISKSTLQLFDYDSSAPPEQAWRRRLNSHANLLKEFRVTFTE 60

Query: 61  AIKM----VRLGIRMWSYVREEASHGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRG 116
           AIKM    VRLGIR+WSYVREEASHGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRG
Sbjct: 61  AIKMASVHVRLGIRIWSYVREEASHGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRG 120

Query: 117 FRGEFRQWQIIPGLCEPSPVMANQFSIFINREGGNKSFASVLAPGQHEGLVGSSKKADDQ 176
           FRGEFRQWQIIP LCE SPVM+NQFSIFI+REGG K FASVLAPGQHEGL     K DDQ
Sbjct: 121 FRGEFRQWQIIPSLCEASPVMSNQFSIFISREGGKKKFASVLAPGQHEGL----GKPDDQ 176

Query: 177 GISSWGWNLNGQHSTYHALFPRAWTIYDGEPDPELKISCRQISPFIPHNYKESSLPAAVF 236
           GISSWGWNL+GQHSTYHALFPRAWT+YDGEPDPELKISCRQISPFIPHNY+ESSLPAAVF
Sbjct: 177 GISSWGWNLSGQHSTYHALFPRAWTVYDGEPDPELKISCRQISPFIPHNYRESSLPAAVF 236

Query: 237 VYTLVNTGKERAKVSLLFTWANSIGGNSHLSGDHVNEPFIAEDGVSGVLLYHK 289
           VYTLVNTGKERAKVSLLFTWANSIGG+SH SGDHVNEPF AEDGVSGVLLYHK
Sbjct: 237 VYTLVNTGKERAKVSLLFTWANSIGGSSHSSGDHVNEPFKAEDGVSGVLLYHK 289


>Glyma07g40310.1 
          Length = 910

 Score =  828 bits (2139), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 451/958 (47%), Positives = 595/958 (62%), Gaps = 74/958 (7%)

Query: 35  PPEQAWRRKLNSHANLLKEFKITFMEAIKMVRLGIRMWSYVREEASHGRKAPIDPFTRES 94
           P    W+RKLN++ N   +  ++  E I +  +G R+W + REEA+ GR   IDPF +  
Sbjct: 6   PAGLTWQRKLNNNGNASSQISLSLKEIIHLAPIGYRLWRHCREEAAKGRGGMIDPFAKRH 65

Query: 95  CKPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGLCEPSPVMANQFSIFINREGGNKSF 154
              +   GVPLGG+G+GSI R FRGEF++WQ+ P +CE  PV+ANQFS+F++R  G K +
Sbjct: 66  V--TFCHGVPLGGIGAGSIGRSFRGEFQRWQLFPVICEEKPVLANQFSVFVSRPSGEK-Y 122

Query: 155 ASVLAPGQHEGLVGSSKKADDQGISSWGWNLNGQHSTYHALFPRAWTIYDGEPDPELKIS 214
           +SVL P +   +    K+    GI SW WN+NG  STYHAL+PRAWT+Y+ EPDP L+I 
Sbjct: 123 SSVLCPRKPVII----KQNPVSGIESWDWNINGNSSTYHALYPRAWTVYE-EPDPALRIV 177

Query: 215 CRQISPFIPHNYKESSLPAAVFVYTLVNTGKERAKVSLLFTWANSIGGNSHLSGDHVNEP 274
           CRQISP IPHNYKESS P  VF +TL N G   A V+LLFTW NS+GG S  +GDH N  
Sbjct: 178 CRQISPVIPHNYKESSFPVTVFTFTLKNLGNTTADVTLLFTWTNSVGGISEFTGDHFNSK 237

Query: 275 FIAEDGVSGVLLYHKTGKCNPPVTFAIAACETQNVSVSVLPSFGLSEG-SSVTAKGMWSK 333
               DGV  VLL+HKT     PVTFAIAA ET++V +S  P F +S   SS++AK MW++
Sbjct: 238 TTRNDGVHAVLLHHKTANERSPVTFAIAAEETEDVHISECPVFVISGAYSSISAKNMWNE 297

Query: 334 MVKDGHFDRENFXXXXXXXXXXXETLCAAVSASTWVEPHGKCTVAFSLAWTSPKVKFVKG 393
           + + G FD  NF            ++ AA++A+  +  + +  V FSLAW  P+VKF +G
Sbjct: 298 VKQHGSFDHLNFAETSAPSEPG-SSIGAAIAATVTIPSNAQRIVTFSLAWDCPEVKFPEG 356

Query: 394 SSFQRRYTKFYGTSDRAAVDLAHDALTHYKRWEEEIEKWQGPVLKDETIPEWYKFTLFNE 453
            ++ RRYTKFYGT+  AA D+AHDA+  + +WE +I+ WQ P+L+D+  PEWY  TL NE
Sbjct: 357 KTYYRRYTKFYGTNGDAAADIAHDAIMEHCQWEAQIDDWQRPILEDKRFPEWYPTTLLNE 416

Query: 454 LYFLVAGGTIWIDSPLQSPLVRSDRDQVNALQNAVVKVTEAKVECRTREVVECXXXXXXX 513
           LY+L +GGTIW D  L  P+            + +V   E K                  
Sbjct: 417 LYYLNSGGTIWTDGSL--PV------------HGLVNTGERKFSLD-------------- 448

Query: 514 XXXXRGHNHVYENHFVDESHENE-SVDTLRRENSIGTHNTSTMMGQ--------QYDDND 564
                G N+       + SH+N+ +++ L   NS+     S    +        Q  + +
Sbjct: 449 -----GFNN-------NLSHKNDIAINILEMFNSVVEQTHSPPASKSAYGVNLLQEGEEN 496

Query: 565 VGRFLYLEGVEYIMWCTYDVHFYASFALLALFPRIELNIQREFAKAVLCEDGRRVKFLAE 624
           +G+FLYLEG+EY MW TYDVHFY+SF+L+ LFP++EL+IQR+FA AVL  D  ++K L +
Sbjct: 497 IGQFLYLEGIEYQMWNTYDVHFYSSFSLVMLFPKLELSIQRDFAAAVLMHDPSKMKLLYD 556

Query: 625 GNWGIRKVYGAVPHDLGTHDPWFEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDLQFG 684
           G W  RKV GAVPHD+G +DPWFE+N YN+++T +WKDLNPKFVLQ+YRD  ATGD +F 
Sbjct: 557 GQWAPRKVLGAVPHDIGLNDPWFEVNGYNLYNTDRWKDLNPKFVLQIYRDVVATGDKKFA 616

Query: 685 VDVWPAVRAAMEYMDQFDRDGDCLIENDGFPDQTYDTWTVHGVSAYCGCXXXXXXXXXXX 744
             VWPAV  A+ YMDQFD+DGD +IEN+GFPDQTYDTW+V GVSAY G            
Sbjct: 617 QAVWPAVYIAIAYMDQFDKDGDGMIENEGFPDQTYDTWSVSGVSAYSGGLWVAALQAASA 676

Query: 745 XXXXXGDRDFAESCKRKFMKAKPVFEEKLWNGSYFNYDSGSSGNSKSIQADQLAGQWYTA 804
                GD+   +    KF KAK V+ E LWNGSYFNYDS    +S SIQADQLAGQWY  
Sbjct: 677 LAREVGDKGSEDYFWLKFQKAKSVY-ENLWNGSYFNYDSSGGSSSLSIQADQLAGQWYAR 735

Query: 805 SSGLPSLFDEPKIKSALRKVYDFNVMKIKGGRMGAVNGMHPNGKVDETCMQSREIWTGVT 864
           + GL  + +E K +SAL+ VY+ NVMK+           H  G VD + MQSREIW+GVT
Sbjct: 736 ACGLLPIVEEKKSRSALQIVYNHNVMKV-----------HKGGVVDMSTMQSREIWSGVT 784

Query: 865 YGVAATMILSGMEEEAFRTAEGIFLAGWSEDGYGYWFQTPEAFTIDGHYRSLIYMRPLAI 924
           Y +AATMI   M + AF+TA G++   WS +G GY FQTPEA+T    YRSL YMRPLAI
Sbjct: 785 YALAATMIQENMIDMAFQTAGGVYETAWSNNGLGYSFQTPEAWTTKDEYRSLCYMRPLAI 844

Query: 925 WGMQYALTLPKAILEAPKMNIMDRIHISPLNGGFSHNETGVRKIATKAKCFSNSVFNC 982
           W MQ+ L+  K I    K ++ ++  +S  + GFS      R +  K +  S S+F  
Sbjct: 845 WAMQWELSRAKHIQHESKSDMKEKDMLSRYHDGFS---KVARLLKVKEETDSRSLFQV 899


>Glyma20g33990.1 
          Length = 964

 Score =  795 bits (2053), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 367/424 (86%), Positives = 393/424 (92%)

Query: 561 DDNDVGRFLYLEGVEYIMWCTYDVHFYASFALLALFPRIELNIQREFAKAVLCEDGRRVK 620
           DD DVGRFLYLEGVEYIMWCTYDVHFYASFALL LFP+IELNIQR+FA+AVLCEDGR+VK
Sbjct: 541 DDADVGRFLYLEGVEYIMWCTYDVHFYASFALLELFPKIELNIQRDFARAVLCEDGRKVK 600

Query: 621 FLAEGNWGIRKVYGAVPHDLGTHDPWFEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGD 680
           FLAEGNWGIRKVYGAVPHDLGTHDPW EMNAYNIHDTSKWKDLNPKFVLQVYRDFA TGD
Sbjct: 601 FLAEGNWGIRKVYGAVPHDLGTHDPWHEMNAYNIHDTSKWKDLNPKFVLQVYRDFATTGD 660

Query: 681 LQFGVDVWPAVRAAMEYMDQFDRDGDCLIENDGFPDQTYDTWTVHGVSAYCGCXXXXXXX 740
           L+FGVDVWPAVRAAMEYM+QFDRDGD LIENDGFPDQTYDTWTVHGVS YCGC       
Sbjct: 661 LEFGVDVWPAVRAAMEYMEQFDRDGDGLIENDGFPDQTYDTWTVHGVSTYCGCLWLAALQ 720

Query: 741 XXXXXXXXXGDRDFAESCKRKFMKAKPVFEEKLWNGSYFNYDSGSSGNSKSIQADQLAGQ 800
                    GDR+FAE CKRKF+KAKP FEEKLWNG+YFNYDSGSSGNSKSIQADQLAGQ
Sbjct: 721 AAAAMALDLGDREFAEKCKRKFLKAKPAFEEKLWNGTYFNYDSGSSGNSKSIQADQLAGQ 780

Query: 801 WYTASSGLPSLFDEPKIKSALRKVYDFNVMKIKGGRMGAVNGMHPNGKVDETCMQSREIW 860
           WYTASSGLP LF++ KIKSALRKVYDFNVMK+KGGRMGAVNGMHPNGKVDETCMQSRE+W
Sbjct: 781 WYTASSGLPPLFEDSKIKSALRKVYDFNVMKVKGGRMGAVNGMHPNGKVDETCMQSREVW 840

Query: 861 TGVTYGVAATMILSGMEEEAFRTAEGIFLAGWSEDGYGYWFQTPEAFTIDGHYRSLIYMR 920
           TGVTYG+AATMIL+GMEEEAF TAEGIFLAGWSEDGYGYWFQTPEA+T+DGHYRSL+YMR
Sbjct: 841 TGVTYGLAATMILAGMEEEAFATAEGIFLAGWSEDGYGYWFQTPEAWTMDGHYRSLMYMR 900

Query: 921 PLAIWGMQYALTLPKAILEAPKMNIMDRIHISPLNGGFSHNETGVRKIATKAKCFSNSVF 980
           PLAIWGMQYA+  PKAILEAPK+NIMDRIH+SP+ GG+SHNETGVRKI TKA+CF+NSVF
Sbjct: 901 PLAIWGMQYAINRPKAILEAPKINIMDRIHLSPVIGGYSHNETGVRKITTKARCFNNSVF 960

Query: 981 NCAC 984
           +CAC
Sbjct: 961 HCAC 964



 Score =  561 bits (1446), Expect = e-159,   Method: Compositional matrix adjust.
 Identities = 268/302 (88%), Positives = 287/302 (95%), Gaps = 2/302 (0%)

Query: 1   MVSGSIFHFRKNSWPAEEYISKSTLQRFDYDSAAPPEQAWRRKLNSHANLLKEFKITFME 60
           MVSG+IFH RKNSWP +EYISKSTLQ FDYDS+APPEQAWRR+LNSHANLLKEF++TFME
Sbjct: 1   MVSGNIFHCRKNSWPPQEYISKSTLQLFDYDSSAPPEQAWRRRLNSHANLLKEFRVTFME 60

Query: 61  AIKMVRLGIRMWSYVREEASHGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIR+WSYVREEASHGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRIWSYVREEASHGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGLCEPSPVMANQFSIFINREGGNKSFASVLAPGQHEGLVGSSKKADDQGISS 180
           FRQWQIIP LCE SPVMANQFSIFI+REGGNK+FASVLAPGQHEGL GSS+K DDQGISS
Sbjct: 121 FRQWQIIPSLCEASPVMANQFSIFISREGGNKNFASVLAPGQHEGL-GSSRKPDDQGISS 179

Query: 181 WGWNLNGQHSTYHALFPRAWTIYDGEPDPELKISCRQISPFIPHNYKESSLPAAVFVYTL 240
           WGWNL+GQHSTYHALFPRAWT+YDGEPDPELKISCRQISPF+PHNY+ESSLPAAVFVYTL
Sbjct: 180 WGWNLSGQHSTYHALFPRAWTVYDGEPDPELKISCRQISPFVPHNYRESSLPAAVFVYTL 239

Query: 241 VNTGKERAKVSLLFTWANSIGGNSHLSGDHVNEPFIAEDGVSGVLLYHK-TGKCNPPVTF 299
           VNTGKERAKVSLLFTWANSIGG+SHLSGDHVNEPF AEDGVSGVLLYHK +   + P++ 
Sbjct: 240 VNTGKERAKVSLLFTWANSIGGSSHLSGDHVNEPFKAEDGVSGVLLYHKQSSASHTPISS 299

Query: 300 AI 301
            I
Sbjct: 300 CI 301



 Score =  295 bits (755), Expect = 2e-79,   Method: Compositional matrix adjust.
 Identities = 149/184 (80%), Positives = 161/184 (87%)

Query: 283 GVLLYHKTGKCNPPVTFAIAACETQNVSVSVLPSFGLSEGSSVTAKGMWSKMVKDGHFDR 342
           G+ +  +T K NPPVTFAIAACETQNV+VSVLPSFGLSEGSS TAKGMWSKMVKDG FD+
Sbjct: 357 GLCVLKRTAKGNPPVTFAIAACETQNVNVSVLPSFGLSEGSSTTAKGMWSKMVKDGQFDQ 416

Query: 343 ENFXXXXXXXXXXXETLCAAVSASTWVEPHGKCTVAFSLAWTSPKVKFVKGSSFQRRYTK 402
           ENF           ETLCAAV+AS WVEPHGKCTVAFSLAW+SPKVKFVKGS+F RRYTK
Sbjct: 417 ENFNSGPSMPSSPGETLCAAVAASMWVEPHGKCTVAFSLAWSSPKVKFVKGSTFNRRYTK 476

Query: 403 FYGTSDRAAVDLAHDALTHYKRWEEEIEKWQGPVLKDETIPEWYKFTLFNELYFLVAGGT 462
           FYGTS++AA DLAHDALTHY RWEEEIEKWQ P+LKDET+PEWYKFTLFNELYFLVAGGT
Sbjct: 477 FYGTSEKAAADLAHDALTHYNRWEEEIEKWQNPILKDETLPEWYKFTLFNELYFLVAGGT 536

Query: 463 IWID 466
           IWID
Sbjct: 537 IWID 540