Miyakogusa Predicted Gene
- Lj4g3v3002630.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj4g3v3002630.1 Non Chatacterized Hit- tr|K4DFT7|K4DFT7_SOLLC
Uncharacterized protein OS=Solanum lycopersicum
GN=Sol,27.32,2e-17,KIP1,KIP1-like; seg,NULL; Prefoldin,Prefoldin;
coiled-coil,NULL,CUFF.52034.1
(621 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma08g02510.1 452 e-127
Glyma05g37050.1 437 e-122
Glyma06g19060.1 122 1e-27
Glyma18g17000.1 75 2e-13
>Glyma08g02510.1
Length = 1302
Score = 452 bits (1162), Expect = e-127, Method: Compositional matrix adjust.
Identities = 304/632 (48%), Positives = 386/632 (61%), Gaps = 78/632 (12%)
Query: 1 MVKHDLRKSIKSFFGSHIDPDKEEQLQEAKTXXXXXXXXXXXXXXXXNVEED-------- 52
MVKH R SIKS FGSHIDP+KEEQLQEAK ++EED
Sbjct: 1 MVKHRFRDSIKSLFGSHIDPNKEEQLQEAKAEIEDKVKRILKLIKEDDLEEDGTPVELSK 60
Query: 53 KEPLVELIEDFHNQYQSLYARYDHLTGELKKRIKGKRESGXXXXXXXXXXXXXXXXX--- 109
KEPLVELIEDFHNQYQSLYA+YDHLT EL+K+I GK+E G
Sbjct: 61 KEPLVELIEDFHNQYQSLYAQYDHLTCELRKKINGKQEKGSSSSSSDSDSDSDYSSKEKD 120
Query: 110 XXNGLPENDFQKIIDGVKQELEMARAEVAEINQKLIVTHEEKEDLNSKYVAALSKIQEAD 169
NG EN+FQK IDG++QELE+ EVAE N+KL +THEEKEDLNSKY+AALSKIQEAD
Sbjct: 121 KKNGQLENEFQKKIDGLRQELEVVHIEVAESNRKLTITHEEKEDLNSKYLAALSKIQEAD 180
Query: 170 KINMDLKTDAEALGIQTSKLLDENAELKKQLDIAGKVEAELSQSLEDLKIEKGSLTMEKE 229
KINMDLKTDAEALG Q SKLL ENAEL KQL+ AGK++AELSQ LEDL EK SLT+EKE
Sbjct: 181 KINMDLKTDAEALGTQRSKLLVENAELNKQLETAGKIKAELSQKLEDLMAEKDSLTIEKE 240
Query: 230 TATQQIDEEKKITDSLRILIDQLKDDKLELGKEFQAVTDELSVVKQHLENAEQKIANVSH 289
TA QQ +EEKKITD LR L+DQLKD+KL LGKE +AV ELS++KQ LE+ EQ++ ++SH
Sbjct: 241 TALQQNEEEKKITDGLRTLVDQLKDEKLALGKELEAVAGELSILKQQLEHTEQQMTDISH 300
Query: 290 NLKITEEENESLKVKLSQASDEVQLAHKKIQESEAELCQLKEKHDVREREVSTLTN---- 345
NLK+ EEENESLKVK SQAS+EVQLAH +IQ+ AE QLKEK D RE+S LT
Sbjct: 301 NLKVAEEENESLKVKHSQASNEVQLAHNRIQDFVAESSQLKEKLDESGREISALTQMHEG 360
Query: 346 ---ESSDQIRELQGKLNKAEEEGAAQLLTFSEKINNLQHDLVSLQNEKQELAQQCEKLKL 402
ESS+QIREL+ +L +L+ +L SLQN+K+++ +Q
Sbjct: 361 YQKESSNQIRELETQL------------------TSLEQELESLQNQKRDMEEQ------ 396
Query: 403 ELDSIHSKKSEAEELLRANVDEISHLTQEKLELSDKIAQFEKRSAERDSEFSALQDKLKK 462
I S +EA EL N L ++I++ E +S ER+ E SA+ KL+
Sbjct: 397 ----IKSSTTEARELGEHNSG-----------LQNQISEHEIKSREREEELSAMMKKLED 441
Query: 463 AEEEGSAQLMALSEQISNLQHDLVSLQNEKQELTEQCEKLKLELDSIHNKKSEAEELLRA 522
E E S+++ L+ QI+ L D+ +L +K EL EQ I +K EA ++
Sbjct: 442 NENESSSKMSDLTSQINKLLADIGTLHAQKNELEEQ----------IISKSDEASTQFKS 491
Query: 523 NSDEISHLTQEKLVLSDKIAQLEKRSAERDSEFSALQDKLKKAEEEGSAQLMALSEQISN 582
++E++ L QE L + + LE + E+ E S E Q+ L E+I
Sbjct: 492 ITNELNALQQEVESLQHQKSDLEVQLVEKIQENS-----------EYVIQIQTLKEEIDR 540
Query: 583 LQHDLVSLQNEKQELTEQCEKLKLELDSIHSK 614
L +K+ L Q L+LE+++I +K
Sbjct: 541 KILGQERLLEDKENLAMQLRTLELEMNTIKNK 572
Score = 215 bits (548), Expect = 1e-55, Method: Compositional matrix adjust.
Identities = 130/265 (49%), Positives = 172/265 (64%), Gaps = 14/265 (5%)
Query: 354 LQGKLNKAEEEGAAQLLTFSEKINNLQHDLVSLQNEKQELAQQCEKLKLELDSIHSKKSE 413
LQ KL++ E E + Q+ +F+ +I+NL+HDLVS+QNEK EL QQCEKLK+ELDS +++K E
Sbjct: 709 LQEKLHEKESEASGQITSFTVQIDNLKHDLVSVQNEKHELEQQCEKLKMELDSTNNRKGE 768
Query: 414 AEELLRANVDEISHLTQEKLELSDKIAQFEKRSAERDSEFSALQDKLKKAEEEGSAQLMA 473
EE L A E + L E L L I EK AE++SE S LQ+KL E + S Q+
Sbjct: 769 IEEQLIAKDRENTKLRGEILGLQGTITALEKTLAEKESELSTLQEKLHANESKASGQITT 828
Query: 474 LSEQISNLQHDLVSLQNEKQELTEQCEKLKLELDSIHNKKSEAEELLRANSDEISHLTQE 533
+ NEK EL +QCEKL++ELDS HN+ E EE +RA E + L +E
Sbjct: 829 FT--------------NEKHELEQQCEKLRMELDSTHNQNGEVEEQMRAKDHENTELREE 874
Query: 534 KLVLSDKIAQLEKRSAERDSEFSALQDKLKKAEEEGSAQLMALSEQISNLQHDLVSLQNE 593
L L I LEK E++SE S LQ+KL + E E S Q+ A + QI NL+HDLVSLQNE
Sbjct: 875 ILGLHGTITALEKTLVEKESELSTLQEKLHEKESEASGQITAFTAQIDNLKHDLVSLQNE 934
Query: 594 KQELTEQCEKLKLELDSIHSKKTKL 618
K EL +QCEKLK+ELDS H++K+++
Sbjct: 935 KHELEQQCEKLKMELDSTHNQKSEV 959
Score = 178 bits (451), Expect = 2e-44, Method: Compositional matrix adjust.
Identities = 128/261 (49%), Positives = 166/261 (63%), Gaps = 14/261 (5%)
Query: 354 LQGKLNKAEEEGAAQLLTFSEKINNLQHDLVSLQNEKQELAQQCEKLKLELDSIHSKKSE 413
LQ K+ E+ +A++ SE+I NL HDL SLQ EKQEL QQCEKLKLE+DS+ ++KSE
Sbjct: 607 LQEKIINTEKAVSAKIQASSEQIKNLGHDLASLQQEKQELEQQCEKLKLEVDSVQNRKSE 666
Query: 414 AEELLRANVDEISHLTQEKLELSDKIAQFEKRSAERDSEFSALQDKLKKAEEEGSAQLMA 473
EE +RA E S L +E L L I EK AE+++E S LQ+KL + E E S Q+ +
Sbjct: 667 VEEQMRAKEHENSGLREENLGLQGTITVLEKTIAEKEAELSTLQEKLHEKESEASGQITS 726
Query: 474 LSEQISNLQHDLVSLQNEKQELTEQCEKLKLELDSIHNKKSEAEELLRANSDEISHLTQE 533
+ QI NL+HDLVS+QNEK EL +QCEKLK+ELDS +N+K E EE L A E + L E
Sbjct: 727 FTVQIDNLKHDLVSVQNEKHELEQQCEKLKMELDSTNNRKGEIEEQLIAKDRENTKLRGE 786
Query: 534 KLVLSDKIAQLEKRSAERDSEFSALQDKLKKAEEEGSAQLMALSEQISNLQHDLVSLQNE 593
L L I LEK AE++SE S LQ+KL E + S Q+ + NE
Sbjct: 787 ILGLQGTITALEKTLAEKESELSTLQEKLHANESKASGQITTFT--------------NE 832
Query: 594 KQELTEQCEKLKLELDSIHSK 614
K EL +QCEKL++ELDS H++
Sbjct: 833 KHELEQQCEKLRMELDSTHNQ 853
Score = 165 bits (417), Expect = 2e-40, Method: Compositional matrix adjust.
Identities = 103/213 (48%), Positives = 133/213 (62%), Gaps = 14/213 (6%)
Query: 354 LQGKLNKAEEEGAAQLLTFSEKINNLQHDLVSLQNEKQELAQQCEKLKLELDSIHSKKSE 413
LQ KL+ E + + Q+ TF+ NEK EL QQCEKL++ELDS H++ E
Sbjct: 811 LQEKLHANESKASGQITTFT--------------NEKHELEQQCEKLRMELDSTHNQNGE 856
Query: 414 AEELLRANVDEISHLTQEKLELSDKIAQFEKRSAERDSEFSALQDKLKKAEEEGSAQLMA 473
EE +RA E + L +E L L I EK E++SE S LQ+KL + E E S Q+ A
Sbjct: 857 VEEQMRAKDHENTELREEILGLHGTITALEKTLVEKESELSTLQEKLHEKESEASGQITA 916
Query: 474 LSEQISNLQHDLVSLQNEKQELTEQCEKLKLELDSIHNKKSEAEELLRANSDEISHLTQE 533
+ QI NL+HDLVSLQNEK EL +QCEKLK+ELDS HN+KSE EE RA E + L +E
Sbjct: 917 FTAQIDNLKHDLVSLQNEKHELEQQCEKLKMELDSTHNQKSEVEEQSRAKGHENTELREE 976
Query: 534 KLVLSDKIAQLEKRSAERDSEFSALQDKLKKAE 566
L L I LEK AE++S+ S LQ+KL++ E
Sbjct: 977 ILGLQGTITALEKTLAEKESDLSTLQEKLREKE 1009
Score = 78.6 bits (192), Expect = 2e-14, Method: Compositional matrix adjust.
Identities = 94/334 (28%), Positives = 148/334 (44%), Gaps = 85/334 (25%)
Query: 354 LQGKLNKAEEEGAAQLLTFSEKINNLQHDLVSLQNEKQELAQQCEKLKLELDSIHSKKSE 413
LQ KL++ E E + Q+ F+ +I+NL+HDLVSLQNEK EL QQCEKLK+ELDS H++KSE
Sbjct: 899 LQEKLHEKESEASGQITAFTAQIDNLKHDLVSLQNEKHELEQQCEKLKMELDSTHNQKSE 958
Query: 414 AEELLRANVDEISHLTQEKLELSDKIAQFEKRSAER------------------------ 449
EE RA E + L +E L L I EK AE+
Sbjct: 959 VEEQSRAKGHENTELREEILGLQGTITALEKTLAEKESDLSTLQEKLREKEKNEKNDMSS 1018
Query: 450 --------------------------DSEFSALQDKLKKAE---EEGSAQLM----ALSE 476
DS F KL+ AE EE + + + +
Sbjct: 1019 RTMDLKRSLEEREESYQKLNIEYKQIDSLFKEWMVKLEVAEKKIEEMAGEFHEGIESKDQ 1078
Query: 477 QISNLQHDLVSLQNEKQELTEQCEKLKLELDSIH---------NKKSEAEELLRANSDEI 527
+I++L+H + L K++L E+ +++ L+++ N+K E L + +E
Sbjct: 1079 KIADLEHTVEEL---KRDLEEKGDEISTSLENVRMLEVKLRLSNQKLRVTEQLLSEKEES 1135
Query: 528 SHLTQEKL-----VLSDKIAQLEKRSAERDSEFSALQDKLKKAEEEGSAQLMALS----- 577
T+EK L D+IA L + F + LK+ + + +S
Sbjct: 1136 FWKTEEKFQQDQRALEDRIATLSAIITANNEAFDEIVSNLKECANSVTTGIETISWKVSD 1195
Query: 578 ------EQISNLQHDLVSLQNEKQELTEQCEKLK 605
+ +SN+ H+L ++ +E+ + E+LK
Sbjct: 1196 DCKNFKDSVSNVSHELGVAKDHVREMKREKEQLK 1229
>Glyma05g37050.1
Length = 1152
Score = 437 bits (1124), Expect = e-122, Method: Compositional matrix adjust.
Identities = 313/686 (45%), Positives = 401/686 (58%), Gaps = 131/686 (19%)
Query: 1 MVKHDLRKSIKSFFGSHIDPDKEEQLQEAKTXXXXXXXXXXXXXXXXNVEED-------- 52
MVKH R+SIKS FGSHID +KEEQLQEAK N+EED
Sbjct: 1 MVKHRFRESIKSLFGSHIDSNKEEQLQEAKAEIEDKVKRILKLIKEDNLEEDGTPVEHSK 60
Query: 53 KEPLVELIEDFHNQYQSLYARYDHLTGELKKRIKGKRESGXXXXXXXXXXXXXXXXX--- 109
KEPLVELIEDFHNQYQSLYA+YDHLTGEL+K+IKGKRE G
Sbjct: 61 KEPLVELIEDFHNQYQSLYAQYDHLTGELRKKIKGKREKGSSSSSSDSDSDSDYSSKDRD 120
Query: 110 XXNGLPENDFQKIIDGVKQELEMARAEVAEINQKLIVTHEEKEDLNSKYVAALSKIQEAD 169
NG EN+FQK I ++QELE+ EVAE+N+KL +THEEKEDLNSKY+AALSKIQEAD
Sbjct: 121 NKNGQLENEFQKTIHDLRQELEVVHIEVAELNRKLTITHEEKEDLNSKYLAALSKIQEAD 180
Query: 170 KINMDLKTDAEALGIQTSKLLDENAELKKQLDIAGKVEAELSQSLEDLKIEKGSLTMEKE 229
KINMDLKTDAEALG Q KLL ENAEL KQLD AGK+E ELSQ LEDL EK SLTMEKE
Sbjct: 181 KINMDLKTDAEALGTQRLKLLVENAELNKQLDTAGKIEVELSQKLEDLTAEKDSLTMEKE 240
Query: 230 TATQQIDEEKKITDSLRILIDQLKDDKLELGKEFQAVTDELSVVKQHLENAEQKIANVSH 289
TA QQI+EEKKITD LR L+DQLKD+ L LGKE +AVT EQ++ ++ H
Sbjct: 241 TALQQIEEEKKITDGLRTLVDQLKDENLALGKELEAVT-------------EQQMTDIRH 287
Query: 290 NLKITEEENESLKVKLSQASDEVQLAHKKIQESEAELCQLKEKHDVREREVSTLTNESSD 349
NLK+ EEENESLKVKLSQAS+EV LAH +IQ+ AE QLKEK D R ESS+
Sbjct: 288 NLKVAEEENESLKVKLSQASNEVHLAHNRIQDFVAESSQLKEKLDER------YQKESSN 341
Query: 350 QIRELQGKLNKAEEEGAAQLLTFSEKINNLQHDLVSLQNEKQELAQQCEKLKLELDSIHS 409
QIREL+ AQ T L+ +L SLQN+K+++ +Q I S
Sbjct: 342 QIRELE-----------AQATT-------LEQELESLQNQKRDMEEQ----------IKS 373
Query: 410 KKSEAEELLRANVDEISHLTQEKLELSDKIAQFEKRSAERDSEFSALQDKLKKAEEEGSA 469
+EA EL N L ++I++ E +S ER+ E SA+ KLK E E S+
Sbjct: 374 STTEAGELGELNSG-----------LQNQISELEIKSREREEELSAMMKKLKDNENESSS 422
Query: 470 QLMALSEQISNLQHDLVSLQNEKQELTEQC------------------EKLKLELDSIHN 511
++ L+ QI L D+ +L +K EL EQ L+ E++S+ +
Sbjct: 423 KMSDLTSQIDKLLADIGTLHAQKNELEEQIISKSDEASTQVKSITNELNALRQEVESLQH 482
Query: 512 KKSEAE----ELLRANSDEISHLTQEKLVLSDKIAQLEKRSAERDS----------EFSA 557
+K + E E ++ NS+ + + K + KI + E+ ++++ E +
Sbjct: 483 QKLDLEFQLVEKVQENSEYVIQMQTLKEEIDRKILEQERLLEDKENLAMKLRTLELEMNT 542
Query: 558 LQDKLKKAEEEGSAQLMALSEQISNL-----------QHDLVSLQN-------------- 592
+++K +AEE Q+ A S +IS++ H LV LQ+
Sbjct: 543 IKNKNSEAEE----QIRAKSHEISHMTEIEKISTDRESHFLV-LQDKFINAEQVVSAKIK 597
Query: 593 EKQELTEQCEKLKLELDSIHSKKTKL 618
EKQEL +QCEK+KLE+DSI ++K+++
Sbjct: 598 EKQELEQQCEKMKLEVDSIQNQKSEI 623
Score = 189 bits (481), Expect = 7e-48, Method: Compositional matrix adjust.
Identities = 141/360 (39%), Positives = 213/360 (59%), Gaps = 17/360 (4%)
Query: 258 ELGKEFQAVTDELSVVKQHLENAEQKIANVSHNLKITEEENESLKVKLSQASDEVQLAHK 317
E + +++T+EL+ ++Q +E+ + + ++ L +EN +++ +E+ +
Sbjct: 458 EASTQVKSITNELNALRQEVESLQHQKLDLEFQLVEKVQENSEYVIQMQTLKEEID---R 514
Query: 318 KIQESEAELCQLKEKHDVRER----EVSTLTNESSDQIRELQGK---------LNKAEEE 364
KI E E L + KE ++ R E++T+ N++S+ +++ K + K +
Sbjct: 515 KILEQE-RLLEDKENLAMKLRTLELEMNTIKNKNSEAEEQIRAKSHEISHMTEIEKISTD 573
Query: 365 GAAQLLTFSEKINNLQHDLVSLQNEKQELAQQCEKLKLELDSIHSKKSEAEELLRANVDE 424
+ L +K N + + + EKQEL QQCEK+KLE+DSI ++KSE EE +RA E
Sbjct: 574 RESHFLVLQDKFINAEQVVSAKIKEKQELEQQCEKMKLEVDSIQNQKSEIEEQMRAKDHE 633
Query: 425 ISHLTQEKLELSDKIAQFEKRSAERDSEFSALQDKLKKAEEEGSAQLMALSEQISNLQHD 484
S L +E L I E AE+++E S+LQ+KL + E E S Q+ A + QI NL+HD
Sbjct: 634 NSGLREENLGFQGTITVQENTLAEKEAELSSLQEKLHEKESEASGQITAFTVQIDNLKHD 693
Query: 485 LVSLQNEKQELTEQCEKLKLELDSIHNKKSEAEELLRANSDEISHLTQEKLVLSDKIAQL 544
LVS QNEKQEL +QCEKLK+ELDS +N+ E EE L A E + L +E L L + IA L
Sbjct: 694 LVSWQNEKQELEQQCEKLKMELDSTNNQTGEIEEQLIAKDHENTELREEILRLQEAIAAL 753
Query: 545 EKRSAERDSEFSALQDKLKKAEEEGSAQLMALSEQISNLQHDLVSLQNEKQELTEQCEKL 604
EK AE++SE S LQ+KL + E E S Q++A + QI NLQ DL+S Q K+EL CEK+
Sbjct: 754 EKTLAEKESELSTLQEKLHEKESEASGQIIAFTSQIDNLQKDLLSFQKTKEELELHCEKI 813
Score = 157 bits (397), Expect = 3e-38, Method: Compositional matrix adjust.
Identities = 109/251 (43%), Positives = 149/251 (59%), Gaps = 29/251 (11%)
Query: 364 EGAAQLLTFSEKINNLQHDLVSLQNEKQELAQQCEKLKLELDSIHSKKSEAEELLRANVD 423
E Q+ T E+I+ + L +K+ LA + L+LE+++I +K SEAEE +RA
Sbjct: 500 EYVIQMQTLKEEIDRKILEQERLLEDKENLAMKLRTLELEMNTIKNKNSEAEEQIRAKSH 559
Query: 424 EISHLTQEKLELSDKIAQFEKRSAERDSEFSALQDKLKKAEEEGSAQLMALSEQISNLQH 483
EISH+T+ EK S +R+S F LQDK AE+ SA++
Sbjct: 560 EISHMTE-----------IEKISTDRESHFLVLQDKFINAEQVVSAKI------------ 596
Query: 484 DLVSLQNEKQELTEQCEKLKLELDSIHNKKSEAEELLRANSDEISHLTQEKLVLSDKIAQ 543
EKQEL +QCEK+KLE+DSI N+KSE EE +RA E S L +E L I
Sbjct: 597 ------KEKQELEQQCEKMKLEVDSIQNQKSEIEEQMRAKDHENSGLREENLGFQGTITV 650
Query: 544 LEKRSAERDSEFSALQDKLKKAEEEGSAQLMALSEQISNLQHDLVSLQNEKQELTEQCEK 603
E AE+++E S+LQ+KL + E E S Q+ A + QI NL+HDLVS QNEKQEL +QCEK
Sbjct: 651 QENTLAEKEAELSSLQEKLHEKESEASGQITAFTVQIDNLKHDLVSWQNEKQELEQQCEK 710
Query: 604 LKLELDSIHSK 614
LK+ELDS +++
Sbjct: 711 LKMELDSTNNQ 721
>Glyma06g19060.1
Length = 147
Score = 122 bits (306), Expect = 1e-27, Method: Compositional matrix adjust.
Identities = 77/157 (49%), Positives = 86/157 (54%), Gaps = 23/157 (14%)
Query: 12 SFFGSHIDPDKEEQLQEAKTXXXXXXXXXXXXXXXXNVEED--------KEPLVELIEDF 63
S FGSHIDP+KEEQLQEAK ++EED KEPLVELIEDF
Sbjct: 2 SLFGSHIDPNKEEQLQEAKAEFEDKVKRILKLIKEDDLEEDGTPIELSKKEPLVELIEDF 61
Query: 64 HNQYQSLYARYDHLTGELKKRIKGKRESGXXXXXXXXXXXXXXXXX---XXNGLPENDFQ 120
HNQY SL K+IKGK E G NG EN+FQ
Sbjct: 62 HNQYPSL------------KKIKGKPEKGSSSSSSDSDSNSDYSSKDKDKKNGQLENEFQ 109
Query: 121 KIIDGVKQELEMARAEVAEINQKLIVTHEEKEDLNSK 157
K IDG++QELEM EVAE+N KL +THEEKEDLNSK
Sbjct: 110 KTIDGLRQELEMVHIEVAELNWKLTITHEEKEDLNSK 146
>Glyma18g17000.1
Length = 70
Score = 75.5 bits (184), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 32/38 (84%), Positives = 38/38 (100%)
Query: 53 KEPLVELIEDFHNQYQSLYARYDHLTGELKKRIKGKRE 90
KEPL+ELIEDFHNQYQSLYA+YDHLTG+L+K+IKGK+E
Sbjct: 30 KEPLIELIEDFHNQYQSLYAQYDHLTGKLRKKIKGKQE 67