Miyakogusa Predicted Gene

Lj4g3v2742940.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2742940.1 tr|G7LE12|G7LE12_MEDTR Cysteine desulfurase
OS=Medicago truncatula GN=MTR_8g093560 PE=3 SV=1,83.7,0,no
description,Pyridoxal phosphate-dependent transferase, major region,
subdomain 1; SUBFAMILY NOT N,gene.g57379.t1.1
         (580 letters)

Database: Glyma1.pep 
           75,778 sequences; 25,431,882 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Glyma05g32450.1                                                       839   0.0  
Glyma08g16610.1                                                       833   0.0  
Glyma09g02450.2                                                        64   4e-10
Glyma09g02450.1                                                        64   4e-10
Glyma15g13350.1                                                        64   6e-10
Glyma01g00280.1                                                        55   2e-07

>Glyma05g32450.1 
          Length = 491

 Score =  839 bits (2168), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 407/511 (79%), Positives = 443/511 (86%), Gaps = 30/511 (5%)

Query: 80  MLHEATEYIKKCLGGGEKDAIIFCGSGTTAAIKRLQEVLGIAVPSIMRERLLKSLTKEER 139
           M+HEA EYIKKCLGGGE DA+I CGSGTTAAIKRLQEV+GIAVPS++RER+LKSL  EER
Sbjct: 1   MVHEAREYIKKCLGGGEDDALILCGSGTTAAIKRLQEVMGIAVPSVLRERVLKSLGTEER 60

Query: 140 WVVFVGPHEHHSNLLSWRQSLAEVIEIGLDDQGLLDMEALKLQLEAFKHSNRPLLGSFSA 199
           WVVFVGPHEHHSNLLSWRQSLAEV+EIGLD +GLLD++ALKLQLEA++ +NRP+LGSFSA
Sbjct: 61  WVVFVGPHEHHSNLLSWRQSLAEVVEIGLDQKGLLDIDALKLQLEAYRDTNRPMLGSFSA 120

Query: 200 CSNVTGIYSDTRAIARLLHQYKGFACFDFAASGPYVEIDMRSGQRDGYDAVFLSTHKFLG 259
           CSNVTGIYSDTRAIA+LLH+YK FACFDFAASGPYVEID+RSG+ DGYDAVFLS HKFLG
Sbjct: 121 CSNVTGIYSDTRAIAQLLHKYKAFACFDFAASGPYVEIDVRSGESDGYDAVFLSPHKFLG 180

Query: 260 GPDSPGVLLMNKALYQLRSSPPSTCGGGTVTYVNSFNEKDTLYLENIEDRENGGTPPIIQ 319
           GPDSPGVLLMNKALY L SSPPSTCGGGTVTYVN FNE+DTLYLENIE+RE GGTPPIIQ
Sbjct: 181 GPDSPGVLLMNKALYLLGSSPPSTCGGGTVTYVNGFNEQDTLYLENIEERETGGTPPIIQ 240

Query: 320 TVRAALAFWVKEYIGYEEIEKREQHYINKALQRLVTNPNIKVLGNLKAKRQAILAFLIYS 379
           TV AALAFWVKEYI YEEIEKREQ YINKA +R  +NPNI+VLGNLKAKRQAIL+FLIYS
Sbjct: 241 TVTAALAFWVKEYISYEEIEKREQLYINKAFERFGSNPNIEVLGNLKAKRQAILSFLIYS 300

Query: 380 TTNE-------CDGDSH---ELNLWQEMGNQRGKPLHGPFVAALLNDLFGIQARGGCACA 429
           TT          DG+     EL+LW E GNQRGKPLHGPFVAALLNDLFGIQARGGCACA
Sbjct: 301 TTTNSSSAGEWSDGNKENEGELDLWAETGNQRGKPLHGPFVAALLNDLFGIQARGGCACA 360

Query: 430 GPYGHDLLNINKSQSLAIRSSVQEGYIGVKPGWTRVSFPYYMSEEDFEYILSCIEFLALY 489
           GPYGH+LL+INKSQSLAIRS+VQEGYIGVKPGWTRVSFPYYMSEEDFEYIL  IEFLA+Y
Sbjct: 361 GPYGHELLHINKSQSLAIRSAVQEGYIGVKPGWTRVSFPYYMSEEDFEYILRAIEFLAVY 420

Query: 490 GQRFFPLYSFNLRNGSWRMKTEEFEALTKEGQHLLGTTLEKVNNDVQEGRGHIRNQSYFD 549
           GQRF PLYSFNL NGSWR+KTE+FEALTKE                       R++SYF+
Sbjct: 421 GQRFIPLYSFNLINGSWRLKTEKFEALTKEDMR--------------------RDESYFE 460

Query: 550 AAKCVASSLPKFPPQGILQEDVDPNVLCFRV 580
            AKCVAS LPKFP QGILQEDVDPN+ CFRV
Sbjct: 461 VAKCVASGLPKFPSQGILQEDVDPNIFCFRV 491


>Glyma08g16610.1 
          Length = 487

 Score =  833 bits (2152), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 405/511 (79%), Positives = 445/511 (87%), Gaps = 34/511 (6%)

Query: 80  MLHEATEYIKKCLGGGEKDAIIFCGSGTTAAIKRLQEVLGIAVPSIMRERLLKSLTKEER 139
           M+HEA+EYIKKCLGGGE DA+I CGSGTTAAIKRLQEV+GIAVPS++RER+LKSL+ EER
Sbjct: 1   MVHEASEYIKKCLGGGEDDALILCGSGTTAAIKRLQEVMGIAVPSVLRERVLKSLSTEER 60

Query: 140 WVVFVGPHEHHSNLLSWRQSLAEVIEIGLDDQGLLDMEALKLQLEAFKHSNRPLLGSFSA 199
           WVVFVGPHEHHSNLLSWRQSLA V+EIGLDD+GLLDM+ALKLQLEA+K +NRP+LGSFSA
Sbjct: 61  WVVFVGPHEHHSNLLSWRQSLAMVVEIGLDDKGLLDMDALKLQLEAYKDTNRPMLGSFSA 120

Query: 200 CSNVTGIYSDTRAIARLLHQYKGFACFDFAASGPYVEIDMRSGQRDGYDAVFLSTHKFLG 259
           CSNVTGIYSDTRAIA+LLH+YK FACFDFAASGPYVEID+RSG+ DGYDAVFLS HKFLG
Sbjct: 121 CSNVTGIYSDTRAIAQLLHRYKAFACFDFAASGPYVEIDVRSGESDGYDAVFLSPHKFLG 180

Query: 260 GPDSPGVLLMNKALYQLRSSPPSTCGGGTVTYVNSFNEKDTLYLENIEDRENGGTPPIIQ 319
           GPDSPGVLLMNKALY L SSPPSTCGGGTVTYVN+FNE+DTLYLENIE+RE GGTPPIIQ
Sbjct: 181 GPDSPGVLLMNKALYLLGSSPPSTCGGGTVTYVNAFNEQDTLYLENIEERETGGTPPIIQ 240

Query: 320 TVRAALAFWVKEYIGYEEIEKREQHYINKALQRLVTNPNIKVLGNLKAKRQAILAFLIYS 379
           TVRAALAFWVKEYI YE+IEKREQ YINKAL+RL +NPNI+VLGNLKAKRQAIL+FLIYS
Sbjct: 241 TVRAALAFWVKEYISYEDIEKREQLYINKALERLGSNPNIEVLGNLKAKRQAILSFLIYS 300

Query: 380 -TTNEC------DGDSH---ELNLWQEMGNQRGKPLHGPFVAALLNDLFGIQARGGCACA 429
            TTN C      DG+     EL+LW E GNQRGKPLHGPFVAALLNDLFGIQARGGCACA
Sbjct: 301 TTTNSCSAGEWSDGNKENEGELDLWAETGNQRGKPLHGPFVAALLNDLFGIQARGGCACA 360

Query: 430 GPYGHDLLNINKSQSLAIRSSVQEGYIGVKPGWTRVSFPYYMSEEDFEYILSCIEFLALY 489
           GPYGH+LL+INKS SLAIRS+VQEGYIGVKPGWTRVSFPYYMSEEDFEYIL  IEF+A+Y
Sbjct: 361 GPYGHELLHINKSHSLAIRSAVQEGYIGVKPGWTRVSFPYYMSEEDFEYILKAIEFIAVY 420

Query: 490 GQRFFPLYSFNLRNGSWRMKTEEFEALTKEGQHLLGTTLEKVNNDVQEGRGHIRNQSYFD 549
           GQRF PLYSFNL NGSWR+KT++FEA TKEG                           F+
Sbjct: 421 GQRFIPLYSFNLINGSWRLKTQKFEAQTKEG------------------------TDKFE 456

Query: 550 AAKCVASSLPKFPPQGILQEDVDPNVLCFRV 580
            AKCVASSL KFP QGILQE+VDPN+ CFR+
Sbjct: 457 VAKCVASSLRKFPSQGILQEEVDPNIFCFRI 487


>Glyma09g02450.2 
          Length = 468

 Score = 64.3 bits (155), Expect = 4e-10,   Method: Compositional matrix adjust.
 Identities = 106/450 (23%), Positives = 174/450 (38%), Gaps = 69/450 (15%)

Query: 5   CNESVEEKLSWLRSQIIGHDAEFDSPFGRR-----KLLYADHTASGRSLHYNENFIINHL 59
            NE+V E    + S  +GH      P   +     KL+Y D+ A+ +        + N+ 
Sbjct: 44  VNETVAEPT--VGSSSLGHSTRPHFPILHQEVNGSKLVYLDNAATSQKPTTVLKALQNYY 101

Query: 60  LPFYGNTHTCDSYVGSRTTKMLHEATEYIKKCLGGGEKDAIIFCGSGTTAAIKRLQEVLG 119
             +  N H    ++ ++ T     A   +   +   +   IIF  + + A I  +    G
Sbjct: 102 EAYNSNVHRGIHFLSAKATDEYESARRKVASFINATDSREIIFTKNASEA-INLVAYSWG 160

Query: 120 IAVPSIMRERLLKSLTKEERWVVFVGPHEHHSNLLSWR---QSLAEVIE-IGLDDQGLLD 175
                      L +L  ++  ++ V   EHHS ++ W+   Q    V+  + L+   + D
Sbjct: 161 -----------LSNLKPDDEIILTVA--EHHSAIVPWQIVAQKTGAVLNFVDLNQDEIPD 207

Query: 176 MEALKLQLEAFKHSNRPLLGSFSACSNVTGIYSDTRAIARLLHQYKGFACFDFAASGPYV 235
           ++ LK  L     S +  +      SNV       R IA+  H        D   S P++
Sbjct: 208 IDKLKEML-----SRKTKIVVVHHVSNVLASVLPIRDIAQWAHDVGAKVLVDACQSVPHM 262

Query: 236 EIDMRSGQRDGYDAVFLSTHKFLGGPDSPGVLLMNKALYQLRSSPPSTCGGGTVTYVNSF 295
            +D++S   D   A   S+HK + GP   G L     L  L S PP   GG  ++ V  +
Sbjct: 263 MVDVQSLNADFLVA---SSHK-MCGPTGIGFLYGKIDL--LSSMPPFLGGGEMISDV--Y 314

Query: 296 NEKDTLYLENIEDRENGGTPPIIQTVRAALAFWVKEYIGYEEIEKREQHYINKALQRLVT 355
            +  T Y E    R   GTP I + +    A      IG + I   E        +RL++
Sbjct: 315 LDHST-YAEP-PSRFEAGTPAIGEAIGLGAAIDYLSGIGMQTIHDYEVELGRYLYERLLS 372

Query: 356 NPNIKVLG---NLKAKRQAILAFLIYSTTNECDGDSHELNLWQEMGNQRGKPLHGPFVAA 412
            PNI++ G   + K +R A+ +F +                         + LH   +A 
Sbjct: 373 VPNIRIYGPAPSEKVERAALCSFNV-------------------------ENLHPTDLAT 407

Query: 413 LLNDLFGIQARGGCACAGPYGHDLLNINKS 442
           LL+   G+  R G  CA P  H  L ++ S
Sbjct: 408 LLDQQHGVAIRSGHHCAQPL-HRYLGVSSS 436


>Glyma09g02450.1 
          Length = 468

 Score = 64.3 bits (155), Expect = 4e-10,   Method: Compositional matrix adjust.
 Identities = 106/450 (23%), Positives = 174/450 (38%), Gaps = 69/450 (15%)

Query: 5   CNESVEEKLSWLRSQIIGHDAEFDSPFGRR-----KLLYADHTASGRSLHYNENFIINHL 59
            NE+V E    + S  +GH      P   +     KL+Y D+ A+ +        + N+ 
Sbjct: 44  VNETVAEPT--VGSSSLGHSTRPHFPILHQEVNGSKLVYLDNAATSQKPTTVLKALQNYY 101

Query: 60  LPFYGNTHTCDSYVGSRTTKMLHEATEYIKKCLGGGEKDAIIFCGSGTTAAIKRLQEVLG 119
             +  N H    ++ ++ T     A   +   +   +   IIF  + + A I  +    G
Sbjct: 102 EAYNSNVHRGIHFLSAKATDEYESARRKVASFINATDSREIIFTKNASEA-INLVAYSWG 160

Query: 120 IAVPSIMRERLLKSLTKEERWVVFVGPHEHHSNLLSWR---QSLAEVIE-IGLDDQGLLD 175
                      L +L  ++  ++ V   EHHS ++ W+   Q    V+  + L+   + D
Sbjct: 161 -----------LSNLKPDDEIILTVA--EHHSAIVPWQIVAQKTGAVLNFVDLNQDEIPD 207

Query: 176 MEALKLQLEAFKHSNRPLLGSFSACSNVTGIYSDTRAIARLLHQYKGFACFDFAASGPYV 235
           ++ LK  L     S +  +      SNV       R IA+  H        D   S P++
Sbjct: 208 IDKLKEML-----SRKTKIVVVHHVSNVLASVLPIRDIAQWAHDVGAKVLVDACQSVPHM 262

Query: 236 EIDMRSGQRDGYDAVFLSTHKFLGGPDSPGVLLMNKALYQLRSSPPSTCGGGTVTYVNSF 295
            +D++S   D   A   S+HK + GP   G L     L  L S PP   GG  ++ V  +
Sbjct: 263 MVDVQSLNADFLVA---SSHK-MCGPTGIGFLYGKIDL--LSSMPPFLGGGEMISDV--Y 314

Query: 296 NEKDTLYLENIEDRENGGTPPIIQTVRAALAFWVKEYIGYEEIEKREQHYINKALQRLVT 355
            +  T Y E    R   GTP I + +    A      IG + I   E        +RL++
Sbjct: 315 LDHST-YAEP-PSRFEAGTPAIGEAIGLGAAIDYLSGIGMQTIHDYEVELGRYLYERLLS 372

Query: 356 NPNIKVLG---NLKAKRQAILAFLIYSTTNECDGDSHELNLWQEMGNQRGKPLHGPFVAA 412
            PNI++ G   + K +R A+ +F +                         + LH   +A 
Sbjct: 373 VPNIRIYGPAPSEKVERAALCSFNV-------------------------ENLHPTDLAT 407

Query: 413 LLNDLFGIQARGGCACAGPYGHDLLNINKS 442
           LL+   G+  R G  CA P  H  L ++ S
Sbjct: 408 LLDQQHGVAIRSGHHCAQPL-HRYLGVSSS 436


>Glyma15g13350.1 
          Length = 468

 Score = 63.5 bits (153), Expect = 6e-10,   Method: Compositional matrix adjust.
 Identities = 104/447 (23%), Positives = 172/447 (38%), Gaps = 63/447 (14%)

Query: 5   CNESVEEKLSWLRSQIIGHDAEFDSPFGRR-----KLLYADHTASGRSLHYNENFIINHL 59
            NE+V E +    S  +GH      P   +     KL+Y D+ A+ +        + N+ 
Sbjct: 44  VNEAVAEPIVGFLS--LGHSTRPHFPILHQEVNGSKLVYLDNAATSQKPTAVLKALQNYY 101

Query: 60  LPFYGNTHTCDSYVGSRTTKMLHEATEYIKKCLGGGEKDAIIFCGSGTTAAIKRLQEVLG 119
             +  N H    ++ +R T     A   +   +   +   I+F  + + A I  +    G
Sbjct: 102 EAYNSNVHRGIHFLSARATDEYESARRKVASFINAIDSREIVFTKNASEA-INLVAYSWG 160

Query: 120 IAVPSIMRERLLKSLTKEERWVVFVGPHEHHSNLLSWR---QSLAEVIE-IGLDDQGLLD 175
                      L +L  ++  ++ V   EHHS ++ W+   Q    V++ + L+   + D
Sbjct: 161 -----------LSNLKPDDEIILTVA--EHHSAIVPWQIVAQKTGAVLKFVDLNQDEIPD 207

Query: 176 MEALKLQLEAFKHSNRPLLGSFSACSNVTGIYSDTRAIARLLHQYKGFACFDFAASGPYV 235
           ++ LK  L     S +  +      SNV       + IA+  H        D   S P++
Sbjct: 208 IDKLKEML-----SRKTKIVVVHHVSNVLASVLPIQDIAQWAHDVGAKVLVDACQSVPHM 262

Query: 236 EIDMRSGQRDGYDAVFLSTHKFLGGPDSPGVLLMNKALYQLRSSPPSTCGGGTVTYVNSF 295
            ID++S   D   A   S+HK + GP   G L     L  L S PP   GG  ++ V  +
Sbjct: 263 MIDVQSLNVDFLVA---SSHK-MCGPTGIGFLYGKIDL--LSSMPPFLGGGEMISDV--Y 314

Query: 296 NEKDTLYLENIEDRENGGTPPIIQTVRAALAFWVKEYIGYEEIEKREQHYINKALQRLVT 355
            +  T Y E    R   GTP I + +    A      IG + I   E        +RL++
Sbjct: 315 LDHST-YAEP-PSRFEAGTPAIGEAIGLGAAIDYLSGIGMQTIHDYEVELGRYLYERLLS 372

Query: 356 NPNIKVLGNLKAKRQAILAFLIYSTTNECDGDSHELNLWQEMGNQRGKPLHGPFVAALLN 415
            PNI++ G   +++    A   ++  N                      LH   +A LL+
Sbjct: 373 VPNIRIYGPAPSEKVERAALCSFNVEN----------------------LHPTDLATLLD 410

Query: 416 DLFGIQARGGCACAGPYGHDLLNINKS 442
              G+  R G  CA P  H  L ++ S
Sbjct: 411 QQHGVAIRSGHHCAQPL-HRYLGVSSS 436


>Glyma01g00280.1 
          Length = 180

 Score = 55.1 bits (131), Expect = 2e-07,   Method: Compositional matrix adjust.
 Identities = 31/54 (57%), Positives = 38/54 (70%), Gaps = 10/54 (18%)

Query: 298 KDTLYLENIEDRENGGTPPIIQTVRAALAFW-VKEYIGYEEIEKREQ----HYI 346
           +DTLYLENIE+RE GGTPPIIQTVRA    W ++ YIG   +  R+Q    HY+
Sbjct: 8   QDTLYLENIEERETGGTPPIIQTVRA----WDLRIYIG-NHLNTRQQQLDKHYL 56