Miyakogusa Predicted Gene

Lj4g3v2693590.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2693590.1 Non Chatacterized Hit- tr|I1K4A3|I1K4A3_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.13289
PE,87.63,0,ZINC-BINDING PROTEIN (YIPPEE), PUTATIVE,NULL; FAD NAD
BINDING OXIDOREDUCTASES,NULL; GCV_T,Glycine cl,CUFF.51518.1
         (420 letters)

Database: Glyma1.pep 
           75,778 sequences; 25,431,882 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Glyma05g30270.1                                                       692   0.0  
Glyma05g30270.3                                                       586   e-167
Glyma05g30270.2                                                       586   e-167
Glyma08g13430.1                                                       195   6e-50
Glyma15g11590.1                                                        69   8e-12
Glyma17g01550.3                                                        66   8e-11
Glyma17g01550.2                                                        66   8e-11
Glyma17g01550.1                                                        66   8e-11
Glyma07g39190.1                                                        64   4e-10

>Glyma05g30270.1 
          Length = 423

 Score =  692 bits (1787), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 327/372 (87%), Positives = 346/372 (93%)

Query: 46  SWSISAHASPFDLSPPPIDHDFLDAVKTAGAEVSGDGIVETFHTDEEALDAADNGVAVVD 105
           SWSI A  SPFDLSPPPIDHDFLD VKTAG E+SG+GI+ETFH D+EAL A DNGV VVD
Sbjct: 52  SWSIGAQPSPFDLSPPPIDHDFLDTVKTAGGEISGEGIIETFHNDDEALAAVDNGVVVVD 111

Query: 106 LSHFGRIRVSGEDRIQFLHNQSTANFECLQPGQGCDTVFVTPTARTIDIAHAWVMKNAIT 165
           LSHFGRIRVSGEDRIQFLHNQSTANFE L  GQGCDTVFVTPTARTIDIAHAW+MKNAIT
Sbjct: 112 LSHFGRIRVSGEDRIQFLHNQSTANFESLHEGQGCDTVFVTPTARTIDIAHAWLMKNAIT 171

Query: 166 LVVSPETCGTITEMLNKYIFFGDKVEIQDITKLTSLFVLVGPKSGQVMENLNLGDLIGKP 225
           LVVSPETC TITEMLNKYIFF DKVEIQDITK TS FVLVGPKSGQVMENLNLGDL+GKP
Sbjct: 172 LVVSPETCTTITEMLNKYIFFADKVEIQDITKQTSFFVLVGPKSGQVMENLNLGDLVGKP 231

Query: 226 YGAHQHFNVDKQPVTIGVGNLLSEDGFSLLMSPAAAPSVWEAILAQGAVPMGSNGWNKLR 285
           YG H HFNVDKQP+TIGVGN++SEDGFSLLMSPAAA S+W+AIL+QGA+PMGSN WNKLR
Sbjct: 232 YGTHLHFNVDKQPITIGVGNIISEDGFSLLMSPAAAASIWKAILSQGAIPMGSNAWNKLR 291

Query: 286 VIRGRPAPGMELTKEFNVLEAHLWNSISLNKGCYKGQETIARLITYDGVKQRLWGFHLSA 345
           +IRGRP PGMELT EFNVLEA LWNS+SLNKGCYKGQETI+RLITYDG+KQRLWGFHLSA
Sbjct: 292 IIRGRPTPGMELTNEFNVLEACLWNSVSLNKGCYKGQETISRLITYDGIKQRLWGFHLSA 351

Query: 346 AAEPGSIITVDGKKIGKLTSYTSGRKQSEHFGLGYIKRQAALEGDTVIVGDNISGTVVEV 405
           AAEPGSIITVDGKK+GKLTSYTSGRKQSEHFGLGYIKR+AA EGDTVIVGDNI GTVVEV
Sbjct: 352 AAEPGSIITVDGKKVGKLTSYTSGRKQSEHFGLGYIKRRAASEGDTVIVGDNIKGTVVEV 411

Query: 406 PFLYQQRPPSGS 417
           PFL QQRPPSGS
Sbjct: 412 PFLLQQRPPSGS 423


>Glyma05g30270.3 
          Length = 371

 Score =  586 bits (1510), Expect = e-167,   Method: Compositional matrix adjust.
 Identities = 274/315 (86%), Positives = 292/315 (92%)

Query: 46  SWSISAHASPFDLSPPPIDHDFLDAVKTAGAEVSGDGIVETFHTDEEALDAADNGVAVVD 105
           SWSI A  SPFDLSPPPIDHDFLD VKTAG E+SG+GI+ETFH D+EAL A DNGV VVD
Sbjct: 52  SWSIGAQPSPFDLSPPPIDHDFLDTVKTAGGEISGEGIIETFHNDDEALAAVDNGVVVVD 111

Query: 106 LSHFGRIRVSGEDRIQFLHNQSTANFECLQPGQGCDTVFVTPTARTIDIAHAWVMKNAIT 165
           LSHFGRIRVSGEDRIQFLHNQSTANFE L  GQGCDTVFVTPTARTIDIAHAW+MKNAIT
Sbjct: 112 LSHFGRIRVSGEDRIQFLHNQSTANFESLHEGQGCDTVFVTPTARTIDIAHAWLMKNAIT 171

Query: 166 LVVSPETCGTITEMLNKYIFFGDKVEIQDITKLTSLFVLVGPKSGQVMENLNLGDLIGKP 225
           LVVSPETC TITEMLNKYIFF DKVEIQDITK TS FVLVGPKSGQVMENLNLGDL+GKP
Sbjct: 172 LVVSPETCTTITEMLNKYIFFADKVEIQDITKQTSFFVLVGPKSGQVMENLNLGDLVGKP 231

Query: 226 YGAHQHFNVDKQPVTIGVGNLLSEDGFSLLMSPAAAPSVWEAILAQGAVPMGSNGWNKLR 285
           YG H HFNVDKQP+TIGVGN++SEDGFSLLMSPAAA S+W+AIL+QGA+PMGSN WNKLR
Sbjct: 232 YGTHLHFNVDKQPITIGVGNIISEDGFSLLMSPAAAASIWKAILSQGAIPMGSNAWNKLR 291

Query: 286 VIRGRPAPGMELTKEFNVLEAHLWNSISLNKGCYKGQETIARLITYDGVKQRLWGFHLSA 345
           +IRGRP PGMELT EFNVLEA LWNS+SLNKGCYKGQETI+RLITYDG+KQRLWGFHLSA
Sbjct: 292 IIRGRPTPGMELTNEFNVLEACLWNSVSLNKGCYKGQETISRLITYDGIKQRLWGFHLSA 351

Query: 346 AAEPGSIITVDGKKI 360
           AAEPGSIITVDGKK+
Sbjct: 352 AAEPGSIITVDGKKV 366


>Glyma05g30270.2 
          Length = 371

 Score =  586 bits (1510), Expect = e-167,   Method: Compositional matrix adjust.
 Identities = 274/315 (86%), Positives = 292/315 (92%)

Query: 46  SWSISAHASPFDLSPPPIDHDFLDAVKTAGAEVSGDGIVETFHTDEEALDAADNGVAVVD 105
           SWSI A  SPFDLSPPPIDHDFLD VKTAG E+SG+GI+ETFH D+EAL A DNGV VVD
Sbjct: 52  SWSIGAQPSPFDLSPPPIDHDFLDTVKTAGGEISGEGIIETFHNDDEALAAVDNGVVVVD 111

Query: 106 LSHFGRIRVSGEDRIQFLHNQSTANFECLQPGQGCDTVFVTPTARTIDIAHAWVMKNAIT 165
           LSHFGRIRVSGEDRIQFLHNQSTANFE L  GQGCDTVFVTPTARTIDIAHAW+MKNAIT
Sbjct: 112 LSHFGRIRVSGEDRIQFLHNQSTANFESLHEGQGCDTVFVTPTARTIDIAHAWLMKNAIT 171

Query: 166 LVVSPETCGTITEMLNKYIFFGDKVEIQDITKLTSLFVLVGPKSGQVMENLNLGDLIGKP 225
           LVVSPETC TITEMLNKYIFF DKVEIQDITK TS FVLVGPKSGQVMENLNLGDL+GKP
Sbjct: 172 LVVSPETCTTITEMLNKYIFFADKVEIQDITKQTSFFVLVGPKSGQVMENLNLGDLVGKP 231

Query: 226 YGAHQHFNVDKQPVTIGVGNLLSEDGFSLLMSPAAAPSVWEAILAQGAVPMGSNGWNKLR 285
           YG H HFNVDKQP+TIGVGN++SEDGFSLLMSPAAA S+W+AIL+QGA+PMGSN WNKLR
Sbjct: 232 YGTHLHFNVDKQPITIGVGNIISEDGFSLLMSPAAAASIWKAILSQGAIPMGSNAWNKLR 291

Query: 286 VIRGRPAPGMELTKEFNVLEAHLWNSISLNKGCYKGQETIARLITYDGVKQRLWGFHLSA 345
           +IRGRP PGMELT EFNVLEA LWNS+SLNKGCYKGQETI+RLITYDG+KQRLWGFHLSA
Sbjct: 292 IIRGRPTPGMELTNEFNVLEACLWNSVSLNKGCYKGQETISRLITYDGIKQRLWGFHLSA 351

Query: 346 AAEPGSIITVDGKKI 360
           AAEPGSIITVDGKK+
Sbjct: 352 AAEPGSIITVDGKKV 366


>Glyma08g13430.1 
          Length = 200

 Score =  195 bits (496), Expect = 6e-50,   Method: Compositional matrix adjust.
 Identities = 111/197 (56%), Positives = 130/197 (65%), Gaps = 22/197 (11%)

Query: 232 FNVDKQPVTIGVGNLLSEDGFSLLMSPAAAPSVWEAILAQGAVPMGSNGWNKLRVIRGRP 291
           F VDKQPVT+GVGN++SEDGFSLLMSP AAPS+W+AIL+QGA+PMGSN WNKLR IRG  
Sbjct: 15  FLVDKQPVTLGVGNIISEDGFSLLMSPGAAPSIWKAILSQGAIPMGSNAWNKLRFIRGTE 74

Query: 292 APGMELTKEFNVLE-------AHLWNSISLNKGCYKGQETIARLITYDGVKQRLWGFHLS 344
                L   F V E         LWNS+SLNK  ++    +           R +G  + 
Sbjct: 75  L--FRLCAYFYVSELVYYSILTCLWNSVSLNK-VFQAHNMME--------SSRGYGDLIF 123

Query: 345 AAAEPGSIITVDGKK----IGKLTSYTSGRKQSEHFGLGYIKRQAALEGDTVIVGDNISG 400
              +  + +    +K    +GKLTSYTSGRKQSEHFGLGYIKR+AA EGDTVIV DNI G
Sbjct: 124 LLLQNLAALLQLMRKRKLMVGKLTSYTSGRKQSEHFGLGYIKRRAASEGDTVIVRDNIKG 183

Query: 401 TVVEVPFLYQQRPPSGS 417
           TVVE PFL Q RPPS S
Sbjct: 184 TVVEAPFLSQLRPPSAS 200


>Glyma15g11590.1 
          Length = 407

 Score = 69.3 bits (168), Expect = 8e-12,   Method: Compositional matrix adjust.
 Identities = 83/335 (24%), Positives = 146/335 (43%), Gaps = 21/335 (6%)

Query: 91  EEALDAADNGVAVVDLSHFGRIRVSGEDRIQFLHNQSTANFECLQPGQGCDTVFVTPTAR 150
           +  L+  +NG ++ D+SH   + + G+D + FL     A+   L PG G  TVF      
Sbjct: 69  DSTLNCRENG-SLFDVSHMCGLSLKGKDSVPFLEKLVIADVAGLAPGTGSLTVFTNEKGG 127

Query: 151 TIDIAHAWVMKNAITLVVSPETC-----GTITEMLNKYIFFGDKVEIQDITKLTSLFVLV 205
            ID +    +K+    +V    C       I E +  +   G  V    I    SL  L 
Sbjct: 128 AIDDSVITKVKDDHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWH-IHDERSLLALQ 186

Query: 206 GPKSGQVMENLNLGDLIGKPYGAHQHFNVDKQPVTIGVGNLLSEDGFSLLMSPAAAPSVW 265
           GP +  V+++L   DL    +G  +  +++     +       EDGF + +   +A  + 
Sbjct: 187 GPLAAPVLQHLTKEDLSKMYFGEFRVLDINGVQCFLTRTGYTGEDGFEISIPSESAVDLA 246

Query: 266 EAIL--AQGAVPM-GSNGWNKLRVIRGRPAPGMELTKEFNVLEAHLWNSISLNK---GCY 319
           +AIL  ++G + + G    + LR+  G    G +L +    +EA L  +I   +   G +
Sbjct: 247 KAILEKSEGKIRLTGLGARDSLRLEAGLCLYGNDLEQHITPIEAGLTWAIGKRRRAEGGF 306

Query: 320 KGQETIARLITYDGVKQRLWGFHLSAAAEPGS---IITVDGKKIGKLTSYTSGRKQSEHF 376
            G + I + +  +G K R  GF  S+   P S   I    G  IG+++S        ++ 
Sbjct: 307 LGADVILKQLE-EGPKIRRVGF-FSSGPPPRSHSEIQDEGGNNIGEVSSGGFSPCLKKNI 364

Query: 377 GLGYIK---RQAALEGDTVIVGDNISGTVVEVPFL 408
            +GY+K    +A  +   +I G +  G V ++PF+
Sbjct: 365 AIGYVKSGLHKAGTKVKIIIRGKSNEGVVTKMPFV 399


>Glyma17g01550.3 
          Length = 407

 Score = 65.9 bits (159), Expect = 8e-11,   Method: Compositional matrix adjust.
 Identities = 85/335 (25%), Positives = 145/335 (43%), Gaps = 21/335 (6%)

Query: 91  EEALDAADNGVAVVDLSHFGRIRVSGEDRIQFLHNQSTANFECLQPGQGCDTVFVTPTAR 150
           +  ++  +NG ++ D+SH   + + G+D   FL     A+   L PG G  TVF      
Sbjct: 69  DSTINCRENG-SLFDVSHMCGLSLKGKDAAPFLEKLVIADVAGLAPGTGTLTVFTNEKGG 127

Query: 151 TIDIAH-AWVMKNAITLVVSP----ETCGTITEMLNKYIFFGDKVEIQDITKLTSLFVLV 205
            ID +    V  + I LVV+     +    I E +  +   G  V    I    SL  L 
Sbjct: 128 AIDDSVITKVTDDHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWH-IHDERSLLALQ 186

Query: 206 GPKSGQVMENLNLGDLIGKPYGAHQHFNVDKQPVTIGVGNLLSEDGFSLLMSPAAAPSVW 265
           GP +  V+++L   DL    +G  +  +++     +       EDGF + +       + 
Sbjct: 187 GPLAAPVLQHLTKEDLSKLYFGEFRVLDINGSECFLTRTGYTGEDGFEISVPSEHGVDLA 246

Query: 266 EAIL--AQGAVPM-GSNGWNKLRVIRGRPAPGMELTKEFNVLEAHLWNSISLNK---GCY 319
           +AIL  ++G V + G    + LR+  G    G ++ +    +EA L  +I   +   G +
Sbjct: 247 KAILEKSEGKVRLTGLGARDSLRLEAGLCLYGNDMEQHITPIEAGLTWAIGKRRRAEGGF 306

Query: 320 KGQETIARLITYDGVKQRLWGFHLSAAAEPGS---IITVDGKKIGKLTSYTSGRKQSEHF 376
            G + I + +  +G K R  GF  S+   P S   I    G  IG++TS        ++ 
Sbjct: 307 LGADVILKQLE-EGPKIRRVGF-FSSGPPPRSHSEIQDEGGNNIGEITSGGFSPCLQKNI 364

Query: 377 GLGYIK---RQAALEGDTVIVGDNISGTVVEVPFL 408
            +GY+K    +AA +   +I G    G V ++PF+
Sbjct: 365 AMGYVKSGLHKAATKVKIIIRGKPNEGVVTKMPFV 399


>Glyma17g01550.2 
          Length = 407

 Score = 65.9 bits (159), Expect = 8e-11,   Method: Compositional matrix adjust.
 Identities = 85/335 (25%), Positives = 145/335 (43%), Gaps = 21/335 (6%)

Query: 91  EEALDAADNGVAVVDLSHFGRIRVSGEDRIQFLHNQSTANFECLQPGQGCDTVFVTPTAR 150
           +  ++  +NG ++ D+SH   + + G+D   FL     A+   L PG G  TVF      
Sbjct: 69  DSTINCRENG-SLFDVSHMCGLSLKGKDAAPFLEKLVIADVAGLAPGTGTLTVFTNEKGG 127

Query: 151 TIDIAH-AWVMKNAITLVVSP----ETCGTITEMLNKYIFFGDKVEIQDITKLTSLFVLV 205
            ID +    V  + I LVV+     +    I E +  +   G  V    I    SL  L 
Sbjct: 128 AIDDSVITKVTDDHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWH-IHDERSLLALQ 186

Query: 206 GPKSGQVMENLNLGDLIGKPYGAHQHFNVDKQPVTIGVGNLLSEDGFSLLMSPAAAPSVW 265
           GP +  V+++L   DL    +G  +  +++     +       EDGF + +       + 
Sbjct: 187 GPLAAPVLQHLTKEDLSKLYFGEFRVLDINGSECFLTRTGYTGEDGFEISVPSEHGVDLA 246

Query: 266 EAIL--AQGAVPM-GSNGWNKLRVIRGRPAPGMELTKEFNVLEAHLWNSISLNK---GCY 319
           +AIL  ++G V + G    + LR+  G    G ++ +    +EA L  +I   +   G +
Sbjct: 247 KAILEKSEGKVRLTGLGARDSLRLEAGLCLYGNDMEQHITPIEAGLTWAIGKRRRAEGGF 306

Query: 320 KGQETIARLITYDGVKQRLWGFHLSAAAEPGS---IITVDGKKIGKLTSYTSGRKQSEHF 376
            G + I + +  +G K R  GF  S+   P S   I    G  IG++TS        ++ 
Sbjct: 307 LGADVILKQLE-EGPKIRRVGF-FSSGPPPRSHSEIQDEGGNNIGEITSGGFSPCLQKNI 364

Query: 377 GLGYIK---RQAALEGDTVIVGDNISGTVVEVPFL 408
            +GY+K    +AA +   +I G    G V ++PF+
Sbjct: 365 AMGYVKSGLHKAATKVKIIIRGKPNEGVVTKMPFV 399


>Glyma17g01550.1 
          Length = 407

 Score = 65.9 bits (159), Expect = 8e-11,   Method: Compositional matrix adjust.
 Identities = 85/335 (25%), Positives = 145/335 (43%), Gaps = 21/335 (6%)

Query: 91  EEALDAADNGVAVVDLSHFGRIRVSGEDRIQFLHNQSTANFECLQPGQGCDTVFVTPTAR 150
           +  ++  +NG ++ D+SH   + + G+D   FL     A+   L PG G  TVF      
Sbjct: 69  DSTINCRENG-SLFDVSHMCGLSLKGKDAAPFLEKLVIADVAGLAPGTGTLTVFTNEKGG 127

Query: 151 TIDIAH-AWVMKNAITLVVSP----ETCGTITEMLNKYIFFGDKVEIQDITKLTSLFVLV 205
            ID +    V  + I LVV+     +    I E +  +   G  V    I    SL  L 
Sbjct: 128 AIDDSVITKVTDDHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWH-IHDERSLLALQ 186

Query: 206 GPKSGQVMENLNLGDLIGKPYGAHQHFNVDKQPVTIGVGNLLSEDGFSLLMSPAAAPSVW 265
           GP +  V+++L   DL    +G  +  +++     +       EDGF + +       + 
Sbjct: 187 GPLAAPVLQHLTKEDLSKLYFGEFRVLDINGSECFLTRTGYTGEDGFEISVPSEHGVDLA 246

Query: 266 EAIL--AQGAVPM-GSNGWNKLRVIRGRPAPGMELTKEFNVLEAHLWNSISLNK---GCY 319
           +AIL  ++G V + G    + LR+  G    G ++ +    +EA L  +I   +   G +
Sbjct: 247 KAILEKSEGKVRLTGLGARDSLRLEAGLCLYGNDMEQHITPIEAGLTWAIGKRRRAEGGF 306

Query: 320 KGQETIARLITYDGVKQRLWGFHLSAAAEPGS---IITVDGKKIGKLTSYTSGRKQSEHF 376
            G + I + +  +G K R  GF  S+   P S   I    G  IG++TS        ++ 
Sbjct: 307 LGADVILKQLE-EGPKIRRVGF-FSSGPPPRSHSEIQDEGGNNIGEITSGGFSPCLQKNI 364

Query: 377 GLGYIK---RQAALEGDTVIVGDNISGTVVEVPFL 408
            +GY+K    +AA +   +I G    G V ++PF+
Sbjct: 365 AMGYVKSGLHKAATKVKIIIRGKPNEGVVTKMPFV 399


>Glyma07g39190.1 
          Length = 407

 Score = 63.5 bits (153), Expect = 4e-10,   Method: Compositional matrix adjust.
 Identities = 83/335 (24%), Positives = 144/335 (42%), Gaps = 21/335 (6%)

Query: 91  EEALDAADNGVAVVDLSHFGRIRVSGEDRIQFLHNQSTANFECLQPGQGCDTVFVTPTAR 150
           +  ++   NG ++ D+SH   + + G+D + FL     A+   L PG G  TVF      
Sbjct: 69  DSTINCRQNG-SLFDVSHMCGLSLKGKDAVPFLEKLVIADVAGLAPGTGTLTVFTNEKGG 127

Query: 151 TIDIAH-AWVMKNAITLVVSP----ETCGTITEMLNKYIFFGDKVEIQDITKLTSLFVLV 205
            ID +    V  + I LVV+     +    I E +  +   G  V    I    SL  L 
Sbjct: 128 AIDDSVITKVTDDHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWH-IHDERSLLALQ 186

Query: 206 GPKSGQVMENLNLGDLIGKPYGAHQHFNVDKQPVTIGVGNLLSEDGFSLLMSPAAAPSVW 265
           GP +  V+++L   DL    +G  +  +++     +       EDGF + +       + 
Sbjct: 187 GPLAAPVLQHLTKEDLSKLFFGEFRVLDINGSQCFLTRTGYTGEDGFEISVPSEHGLDLA 246

Query: 266 EAIL--AQGAVPM-GSNGWNKLRVIRGRPAPGMELTKEFNVLEAHLWNSISLNK---GCY 319
           +AIL  ++G V + G    + LR+  G    G ++ +    +EA L  +I   +   G +
Sbjct: 247 KAILEKSEGKVRLTGLGARDSLRLEAGLCLYGNDMEQHITPIEAGLTWAIGKRRRAEGGF 306

Query: 320 KGQETIARLITYDGVKQRLWGFHLSAAAEPGS---IITVDGKKIGKLTSYTSGRKQSEHF 376
            G + I + +  +G K R  GF  S+   P S   I    G  IG++TS        ++ 
Sbjct: 307 LGADVILKQLE-EGPKIRRVGF-FSSGPPPRSHSEIQDEGGNNIGEITSGGFSPCLQKNI 364

Query: 377 GLGYIK---RQAALEGDTVIVGDNISGTVVEVPFL 408
            +GY+K    +A  +   +I G    G + ++PF+
Sbjct: 365 AMGYVKSGLHKAGTKVKIIIRGKPNEGVLTKMPFV 399