Miyakogusa Predicted Gene
- Lj4g3v1511450.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj4g3v1511450.1 Non Chatacterized Hit- tr|I3SR96|I3SR96_MEDTR
Uncharacterized protein OS=Medicago truncatula PE=2
SV,88.84,0,Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal
domain,NULL; NAD(P)-binding Rossmann-fold ,CUFF.49329.1
(472 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma05g02190.1 877 0.0
Glyma17g09740.1 862 0.0
Glyma16g10880.1 743 0.0
Glyma16g10880.3 722 0.0
Glyma16g10880.2 659 0.0
Glyma15g18270.1 265 7e-71
Glyma04g21480.1 80 4e-15
Glyma18g22140.1 53 9e-07
>Glyma05g02190.1
Length = 475
Score = 877 bits (2265), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 426/474 (89%), Positives = 448/474 (94%), Gaps = 2/474 (0%)
Query: 1 MALNLSSPAEVKSSFFTDSFNSSCFTPKFPVSFALKRKGCGGVSGRRIHCSVQT--PPPA 58
MALN+SSPAEVKS FF DSF S+C T KF FA KRK SG R++CSVQ PPPA
Sbjct: 2 MALNISSPAEVKSIFFADSFKSNCLTAKFSGGFAFKRKERRAASGGRVYCSVQATPPPPA 61
Query: 59 WPGRAVVEPGRKTWEGPKPISIVGCTGSIGTQTLDIVAENPDKFRVVALAAGSNITLLAD 118
WPGRAV E GRKTW+GPKPISIVG TGSIGTQTLDIVAENPDKF+VVALAAGSN+TLLAD
Sbjct: 62 WPGRAVPEQGRKTWDGPKPISIVGSTGSIGTQTLDIVAENPDKFKVVALAAGSNVTLLAD 121
Query: 119 QVRRFKPQLVAVRNESLISELEEALNDLEEKPEIIPGEQGVIEVARHPDAVSVVTGIVGC 178
QV+RFKPQLVAVRNESLI+ELEEAL+D+EEKPEIIPGEQG+IEVARHPDAVSVVTGIVGC
Sbjct: 122 QVKRFKPQLVAVRNESLIAELEEALHDVEEKPEIIPGEQGIIEVARHPDAVSVVTGIVGC 181
Query: 179 AGLKPTVAAIEAGKDIALANKETLIAGGPFVLPLAQKHNIKILPADSEHSAIFQCIQGLS 238
AGLKPTVAAIEAGKDIALANKETLIAGGPFVLPLAQKHN+KILPADSEHSAIFQCIQGL
Sbjct: 182 AGLKPTVAAIEAGKDIALANKETLIAGGPFVLPLAQKHNVKILPADSEHSAIFQCIQGLP 241
Query: 239 EGALRRVILTASGGAFRDWPADKLKDVKVADALKHPNWNMGKKITVDSATLFNKGLEVIE 298
EGALRRVILTASGGAFRDWP DKLKDVKVADALKHPNWNMGKKITVDSATLFNKGLEVIE
Sbjct: 242 EGALRRVILTASGGAFRDWPVDKLKDVKVADALKHPNWNMGKKITVDSATLFNKGLEVIE 301
Query: 299 AHYLFGADYDNIDIVIHPQSIIHSMIETQDSSVLAQLGWPDMRLPILYTLSWPDRIYCSE 358
AHYLFGADYD+I+IVIHPQSIIHSMIETQDSSVLAQLGWPDMRLPILYTLSWPDRIYCSE
Sbjct: 302 AHYLFGADYDHIEIVIHPQSIIHSMIETQDSSVLAQLGWPDMRLPILYTLSWPDRIYCSE 361
Query: 359 VTWPRLDLCKLGSLTFKAPDNVKYPSVNLAYAAGRAGGTMTGVLSAANEKAVEMFIDEKI 418
VTWPRLDLCKLGSLTFK PDNVKYPS+NLA++AGRAGGTMTGVLSAANEKAVEMFIDEKI
Sbjct: 362 VTWPRLDLCKLGSLTFKTPDNVKYPSMNLAFSAGRAGGTMTGVLSAANEKAVEMFIDEKI 421
Query: 419 SYLDIFKVVELTCEKHQNELRTSPSLEEIIHYDLWAREYAASLQNTSSFTPIPA 472
SYLDIFKVVELTCEKHQNEL +SPSLEEIIHYDLWAR+YAASLQ++SSFTPI A
Sbjct: 422 SYLDIFKVVELTCEKHQNELVSSPSLEEIIHYDLWARKYAASLQDSSSFTPILA 475
>Glyma17g09740.1
Length = 474
Score = 862 bits (2228), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 422/473 (89%), Positives = 443/473 (93%), Gaps = 1/473 (0%)
Query: 1 MALNLSSPAEVKSSFFTDSFNSSCFTPKFPVSFALKRKGCGGVSGRRIHCSVQTPPP-AW 59
MALN+SSPAEVKS FF D F S+C T KF FA KRK G SG R+ CSV PPP AW
Sbjct: 2 MALNISSPAEVKSIFFADYFKSNCITAKFTGGFAFKRKERGAASGGRVCCSVLAPPPPAW 61
Query: 60 PGRAVVEPGRKTWEGPKPISIVGCTGSIGTQTLDIVAENPDKFRVVALAAGSNITLLADQ 119
PGRAV E G KTW+GPKPISIVGCTGSIGTQTLDIVAENPDKF+VVALAAGSN+TLLADQ
Sbjct: 62 PGRAVPEQGSKTWDGPKPISIVGCTGSIGTQTLDIVAENPDKFKVVALAAGSNVTLLADQ 121
Query: 120 VRRFKPQLVAVRNESLISELEEALNDLEEKPEIIPGEQGVIEVARHPDAVSVVTGIVGCA 179
V+RFKPQLVAVRNESLI+EL+EAL+D+EEKPEIIPGEQG+IEVARHPDAVSVVTGIVGCA
Sbjct: 122 VKRFKPQLVAVRNESLIAELKEALHDVEEKPEIIPGEQGIIEVARHPDAVSVVTGIVGCA 181
Query: 180 GLKPTVAAIEAGKDIALANKETLIAGGPFVLPLAQKHNIKILPADSEHSAIFQCIQGLSE 239
GLKPTVAAIEAGK IALANKETLIAGGPFVLPLAQKHN+KILPADSEHSAIFQCIQGL E
Sbjct: 182 GLKPTVAAIEAGKYIALANKETLIAGGPFVLPLAQKHNVKILPADSEHSAIFQCIQGLPE 241
Query: 240 GALRRVILTASGGAFRDWPADKLKDVKVADALKHPNWNMGKKITVDSATLFNKGLEVIEA 299
GALRRVILTASGGAFRDWP DKLKDVKVADALKHPNWNMGKKITVDSATLFNKGLEVIEA
Sbjct: 242 GALRRVILTASGGAFRDWPVDKLKDVKVADALKHPNWNMGKKITVDSATLFNKGLEVIEA 301
Query: 300 HYLFGADYDNIDIVIHPQSIIHSMIETQDSSVLAQLGWPDMRLPILYTLSWPDRIYCSEV 359
HYLFGADYD+I+IVIHPQSIIHSMIETQDSSVLAQLGWPDMRLPILYTLSWPDRIYCSE
Sbjct: 302 HYLFGADYDHIEIVIHPQSIIHSMIETQDSSVLAQLGWPDMRLPILYTLSWPDRIYCSED 361
Query: 360 TWPRLDLCKLGSLTFKAPDNVKYPSVNLAYAAGRAGGTMTGVLSAANEKAVEMFIDEKIS 419
TWP LDLCK+GSLTFK PDNVKYPS+NLAYAAGRAGGTMTGVLSAANEKAVEMFIDEKIS
Sbjct: 362 TWPCLDLCKIGSLTFKTPDNVKYPSMNLAYAAGRAGGTMTGVLSAANEKAVEMFIDEKIS 421
Query: 420 YLDIFKVVELTCEKHQNELRTSPSLEEIIHYDLWAREYAASLQNTSSFTPIPA 472
YLDIFKVVELTCEKHQNEL +SPSLEEIIHYDLWARE+AASLQ++SSFTPI A
Sbjct: 422 YLDIFKVVELTCEKHQNELLSSPSLEEIIHYDLWAREFAASLQDSSSFTPILA 474
>Glyma16g10880.1
Length = 465
Score = 743 bits (1919), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 361/469 (76%), Positives = 413/469 (88%), Gaps = 8/469 (1%)
Query: 1 MALNLSSPAEVKSSFFTDSFNSSCFTPKFPVSFALKRKGCGGVSGRRIHCSV--QTPPPA 58
MALNL SPA+VK FF+ S NS+ K P SF+LKRK RR++CS Q+PPPA
Sbjct: 1 MALNLPSPAQVKPLFFS-SNNST----KLPGSFSLKRKDSDTTVERRVYCSAAAQSPPPA 55
Query: 59 WPGRAVVEPGR-KTWEGPKPISIVGCTGSIGTQTLDIVAENPDKFRVVALAAGSNITLLA 117
WPG A+ EP KTW+G KPIS++G TGSIGTQTL IVAE P++F+VV+LAAGSNITLLA
Sbjct: 56 WPGTAIPEPSDFKTWDGQKPISVLGSTGSIGTQTLSIVAEFPERFKVVSLAAGSNITLLA 115
Query: 118 DQVRRFKPQLVAVRNESLISELEEALNDLEEKPEIIPGEQGVIEVARHPDAVSVVTGIVG 177
DQ++ FKP++V +RNESLI EL+EAL D++ KPEIIPGEQGVIE ARHPDA +VVTGIVG
Sbjct: 116 DQIKTFKPEVVGLRNESLIDELKEALADVDHKPEIIPGEQGVIEAARHPDATTVVTGIVG 175
Query: 178 CAGLKPTVAAIEAGKDIALANKETLIAGGPFVLPLAQKHNIKILPADSEHSAIFQCIQGL 237
CAGLKPTVAAIEAGKDIALANKET+IAG PFVLPLA KHNIKILPADSEHSAIFQ IQGL
Sbjct: 176 CAGLKPTVAAIEAGKDIALANKETMIAGAPFVLPLAHKHNIKILPADSEHSAIFQSIQGL 235
Query: 238 SEGALRRVILTASGGAFRDWPADKLKDVKVADALKHPNWNMGKKITVDSATLFNKGLEVI 297
+GALR+++LT SGGAFR+WPA+K+KD+K+ADALKHP W++G+KIT+DSATLFNKGLEVI
Sbjct: 236 PKGALRKILLTGSGGAFREWPAEKMKDIKLADALKHPIWSLGRKITIDSATLFNKGLEVI 295
Query: 298 EAHYLFGADYDNIDIVIHPQSIIHSMIETQDSSVLAQLGWPDMRLPILYTLSWPDRIYCS 357
EAHYLFGA YD+I+IVIHPQSIIHS++ETQDSSV+AQLG PDMRLP+LYTLSWP+RIYCS
Sbjct: 296 EAHYLFGASYDDIEIVIHPQSIIHSLVETQDSSVIAQLGIPDMRLPLLYTLSWPERIYCS 355
Query: 358 EVTWPRLDLCKLGSLTFKAPDNVKYPSVNLAYAAGRAGGTMTGVLSAANEKAVEMFIDEK 417
EVTWPRLDL K GSLTF APD+ K+PSVNL YAAGRAGGTMTGVLSAANEKAVEMF++EK
Sbjct: 356 EVTWPRLDLSKYGSLTFYAPDDKKFPSVNLCYAAGRAGGTMTGVLSAANEKAVEMFVEEK 415
Query: 418 ISYLDIFKVVELTCEKHQNELRTSPSLEEIIHYDLWAREYAASLQNTSS 466
ISYLDIFKVVELTC++HQ EL SPSLEEIIHYD WAR+YAASLQ SS
Sbjct: 416 ISYLDIFKVVELTCQEHQKELVASPSLEEIIHYDQWARQYAASLQKASS 464
>Glyma16g10880.3
Length = 463
Score = 722 bits (1864), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 344/438 (78%), Positives = 393/438 (89%), Gaps = 3/438 (0%)
Query: 32 SFALKRKGCGGVSGRRIHCSV--QTPPPAWPGRAVVEPGR-KTWEGPKPISIVGCTGSIG 88
SF+LKRK RR++CS Q+PPPAWPG A+ EP KTW+G KPIS++G TGSIG
Sbjct: 25 SFSLKRKDSDTTVERRVYCSAAAQSPPPAWPGTAIPEPSDFKTWDGQKPISVLGSTGSIG 84
Query: 89 TQTLDIVAENPDKFRVVALAAGSNITLLADQVRRFKPQLVAVRNESLISELEEALNDLEE 148
TQTL IVAE P++F+VV+LAAGSNITLLADQ++ FKP++V +RNESLI EL+EAL D++
Sbjct: 85 TQTLSIVAEFPERFKVVSLAAGSNITLLADQIKTFKPEVVGLRNESLIDELKEALADVDH 144
Query: 149 KPEIIPGEQGVIEVARHPDAVSVVTGIVGCAGLKPTVAAIEAGKDIALANKETLIAGGPF 208
KPEIIPGEQGVIE ARHPDA +VVTGIVGCAGLKPTVAAIEAGKDIALANKET+IAG PF
Sbjct: 145 KPEIIPGEQGVIEAARHPDATTVVTGIVGCAGLKPTVAAIEAGKDIALANKETMIAGAPF 204
Query: 209 VLPLAQKHNIKILPADSEHSAIFQCIQGLSEGALRRVILTASGGAFRDWPADKLKDVKVA 268
VLPLA KHNIKILPADSEHSAIFQ IQGL +GALR+++LT SGGAFR+WPA+K+KD+K+A
Sbjct: 205 VLPLAHKHNIKILPADSEHSAIFQSIQGLPKGALRKILLTGSGGAFREWPAEKMKDIKLA 264
Query: 269 DALKHPNWNMGKKITVDSATLFNKGLEVIEAHYLFGADYDNIDIVIHPQSIIHSMIETQD 328
DALKHP W++G+KIT+DSATLFNKGLEVIEAHYLFGA YD+I+IVIHPQSIIHS++ETQD
Sbjct: 265 DALKHPIWSLGRKITIDSATLFNKGLEVIEAHYLFGASYDDIEIVIHPQSIIHSLVETQD 324
Query: 329 SSVLAQLGWPDMRLPILYTLSWPDRIYCSEVTWPRLDLCKLGSLTFKAPDNVKYPSVNLA 388
SSV+AQLG PDMRLP+LYTLSWP+RIYCSEVTWPRLDL K GSLTF APD+ K+PSVNL
Sbjct: 325 SSVIAQLGIPDMRLPLLYTLSWPERIYCSEVTWPRLDLSKYGSLTFYAPDDKKFPSVNLC 384
Query: 389 YAAGRAGGTMTGVLSAANEKAVEMFIDEKISYLDIFKVVELTCEKHQNELRTSPSLEEII 448
YAAGRAGGTMTGVLSAANEKAVEMF++EKISYLDIFKVVELTC++HQ EL SPSLEEII
Sbjct: 385 YAAGRAGGTMTGVLSAANEKAVEMFVEEKISYLDIFKVVELTCQEHQKELVASPSLEEII 444
Query: 449 HYDLWAREYAASLQNTSS 466
HYD WAR+YAASLQ SS
Sbjct: 445 HYDQWARQYAASLQKASS 462
>Glyma16g10880.2
Length = 415
Score = 659 bits (1701), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 321/420 (76%), Positives = 370/420 (88%), Gaps = 8/420 (1%)
Query: 1 MALNLSSPAEVKSSFFTDSFNSSCFTPKFPVSFALKRKGCGGVSGRRIHCSV--QTPPPA 58
MALNL SPA+VK FF+ S NS+ K P SF+LKRK RR++CS Q+PPPA
Sbjct: 1 MALNLPSPAQVKPLFFS-SNNST----KLPGSFSLKRKDSDTTVERRVYCSAAAQSPPPA 55
Query: 59 WPGRAVVEPGR-KTWEGPKPISIVGCTGSIGTQTLDIVAENPDKFRVVALAAGSNITLLA 117
WPG A+ EP KTW+G KPIS++G TGSIGTQTL IVAE P++F+VV+LAAGSNITLLA
Sbjct: 56 WPGTAIPEPSDFKTWDGQKPISVLGSTGSIGTQTLSIVAEFPERFKVVSLAAGSNITLLA 115
Query: 118 DQVRRFKPQLVAVRNESLISELEEALNDLEEKPEIIPGEQGVIEVARHPDAVSVVTGIVG 177
DQ++ FKP++V +RNESLI EL+EAL D++ KPEIIPGEQGVIE ARHPDA +VVTGIVG
Sbjct: 116 DQIKTFKPEVVGLRNESLIDELKEALADVDHKPEIIPGEQGVIEAARHPDATTVVTGIVG 175
Query: 178 CAGLKPTVAAIEAGKDIALANKETLIAGGPFVLPLAQKHNIKILPADSEHSAIFQCIQGL 237
CAGLKPTVAAIEAGKDIALANKET+IAG PFVLPLA KHNIKILPADSEHSAIFQ IQGL
Sbjct: 176 CAGLKPTVAAIEAGKDIALANKETMIAGAPFVLPLAHKHNIKILPADSEHSAIFQSIQGL 235
Query: 238 SEGALRRVILTASGGAFRDWPADKLKDVKVADALKHPNWNMGKKITVDSATLFNKGLEVI 297
+GALR+++LT SGGAFR+WPA+K+KD+K+ADALKHP W++G+KIT+DSATLFNKGLEVI
Sbjct: 236 PKGALRKILLTGSGGAFREWPAEKMKDIKLADALKHPIWSLGRKITIDSATLFNKGLEVI 295
Query: 298 EAHYLFGADYDNIDIVIHPQSIIHSMIETQDSSVLAQLGWPDMRLPILYTLSWPDRIYCS 357
EAHYLFGA YD+I+IVIHPQSIIHS++ETQDSSV+AQLG PDMRLP+LYTLSWP+RIYCS
Sbjct: 296 EAHYLFGASYDDIEIVIHPQSIIHSLVETQDSSVIAQLGIPDMRLPLLYTLSWPERIYCS 355
Query: 358 EVTWPRLDLCKLGSLTFKAPDNVKYPSVNLAYAAGRAGGTMTGVLSAANEKAVEMFIDEK 417
EVTWPRLDL K GSLTF APD+ K+PSVNL YAAGRAGGTMTGVLSAANEKAVEMF++EK
Sbjct: 356 EVTWPRLDLSKYGSLTFYAPDDKKFPSVNLCYAAGRAGGTMTGVLSAANEKAVEMFVEEK 415
>Glyma15g18270.1
Length = 361
Score = 265 bits (677), Expect = 7e-71, Method: Compositional matrix adjust.
Identities = 160/284 (56%), Positives = 178/284 (62%), Gaps = 53/284 (18%)
Query: 169 VSVVTGIVGCAGLKPTVAAIEAGKDIALANKETLIAGGPFVLPLAQKHNIKILPADSEHS 228
+SVVT IVGCAGLKPTVA I+A DIALANK TLI GGPFVLP Q+HNIK++ DSEH
Sbjct: 111 ISVVTRIVGCAGLKPTVAMIKAWTDIALANKGTLIVGGPFVLP-PQQHNIKLVTNDSEHY 169
Query: 229 AIFQCIQGLSEGALRRVILTASGGAFRDWPADKLKDVKVADALKHPNWNMGKKITVDSAT 288
IFQCIQGL +GAL ++ILTASGGAF DWP DKLK VKVA+AL W +
Sbjct: 170 IIFQCIQGLPKGALMQIILTASGGAFGDWPIDKLKAVKVANAL----WIL--------LY 217
Query: 289 LFNKGLEVIEAHYLFGADYDNIDIVIHPQSIIHSMIETQDSSVLAQLGWPDMRLPILYTL 348
K LEVI+A YLFGADYD+I+I IHP+S IH + L+
Sbjct: 218 FLIKDLEVIDADYLFGADYDHIEIFIHPKSSIHRI--------------------YLFLH 257
Query: 349 SWPDRIYCSEVTWPRLDLCKLGSLTFKAPDNVKYPSVNLAYAAGRAGGTMTGVLSAANEK 408
W I + P LGSLTF KYPS+NLAY G NEK
Sbjct: 258 IWGALICVCQS--PLHSHGLLGSLTF------KYPSMNLAYVVG------------PNEK 297
Query: 409 AVEMFIDEKISYLDIFKVVELTCEKHQNELRTSPSLEEIIHYDL 452
EMFIDEKISYLDI KVVELTCEKHQNEL SPSLEEIIHY L
Sbjct: 298 VAEMFIDEKISYLDILKVVELTCEKHQNELLASPSLEEIIHYYL 341
>Glyma04g21480.1
Length = 51
Score = 80.5 bits (197), Expect = 4e-15, Method: Compositional matrix adjust.
Identities = 40/52 (76%), Positives = 46/52 (88%), Gaps = 1/52 (1%)
Query: 181 LKPTVAAIEAGKDIALANKETLIAGGPFVLPLAQKHNIKILPADSEHSAIFQ 232
L+PTVAAIEAGKDIALANK+ +I G PFV P + KHNI+ILPA+SEHSAIFQ
Sbjct: 1 LQPTVAAIEAGKDIALANKDEMIVGAPFV-PPSHKHNIQILPANSEHSAIFQ 51
>Glyma18g22140.1
Length = 168
Score = 52.8 bits (125), Expect = 9e-07, Method: Compositional matrix adjust.
Identities = 24/40 (60%), Positives = 27/40 (67%)
Query: 62 RAVVEPGRKTWEGPKPISIVGCTGSIGTQTLDIVAENPDK 101
R V+ G KTW GPK ISIVGCTGSIGTQ V++ K
Sbjct: 67 RIVIFDGSKTWAGPKTISIVGCTGSIGTQATTFVSKGEKK 106