Miyakogusa Predicted Gene

Lj4g3v1288450.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v1288450.1 Non Chatacterized Hit- tr|I3S559|I3S559_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,99.52,0,BILE
ACID:SODIUM SYMPORTER FAMILY PROTEIN,NULL; SODIUM-BILE ACID
COTRANSPORTER,Bile acid:sodium
symp,NODE_74711_length_1443_cov_20.528067.path2.1
         (414 letters)

Database: Glyma1.pep 
           75,778 sequences; 25,431,882 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Glyma01g42260.1                                                       484   e-136
Glyma11g03100.1                                                       454   e-128
Glyma01g42260.2                                                       443   e-124
Glyma08g45790.1                                                       169   4e-42
Glyma17g12890.1                                                       141   1e-33
Glyma08g45790.2                                                       135   7e-32
Glyma13g33940.1                                                       135   1e-31
Glyma05g08120.1                                                       132   5e-31
Glyma12g36310.1                                                       124   2e-28

>Glyma01g42260.1 
          Length = 408

 Score =  484 bits (1245), Expect = e-136,   Method: Compositional matrix adjust.
 Identities = 253/391 (64%), Positives = 294/391 (75%), Gaps = 6/391 (1%)

Query: 24  KSPVSVRCRTNVTSPFSIHLRPHLPPHWSPKLPLKCAPLTSSDSLGPDQFPVHSGDLTQT 83
           K P+ + C   ++SP SI    H P + S K+PLKCAPL SSDSL PD     S   TQT
Sbjct: 24  KPPILLPCCPTISSPCSIRFNSHFP-YRSTKVPLKCAPLPSSDSLPPDL----SDAPTQT 78

Query: 84  LKQNWVSFLEILKQSNSLLPYVALTSTLIALVIPQSFTWFTTRHYAPALGFLMFAVGVNS 143
            +QN +S LEILKQSNS LP+V + S L+AL+ P S TWFT+R+YAPALGFLMFAVGVNS
Sbjct: 79  -EQNSMSILEILKQSNSYLPHVLIASILLALIYPPSLTWFTSRYYAPALGFLMFAVGVNS 137

Query: 144 KEKDFLDAFKRPTELLTAYACQFILKPLLGYLLCIISVNVFGLPTAIGAGIVLLSCVSGA 203
            E DFL+AFKRP E++T Y  QF +KPLLGYL C+I+V V  LPT +GAGIVL++CVSGA
Sbjct: 138 NENDFLEAFKRPAEIVTGYFGQFAVKPLLGYLFCMIAVTVLSLPTTVGAGIVLVACVSGA 197

Query: 204 QLSSYATFLSDPEMAPLSIVMTSLSTVSAVFVTPXXXXXXXGKRLPIDVKGMVFSITQIV 263
           QLSSYATFL+DP+MAPLSIVMTSLST SAVFVTP       GK+LPIDV+GMV+SITQIV
Sbjct: 198 QLSSYATFLTDPQMAPLSIVMTSLSTASAVFVTPLLLLLLIGKKLPIDVRGMVYSITQIV 257

Query: 264 LVPITGGLLLNRFFPHICNSIRXXXXXXXXXXXXXXXXXXIAHNIESVKSPFGVAIFLLA 323
           +VPI  GLLLNRF+P ICN IR                  +A N+E++KSP GVAI LL 
Sbjct: 258 VVPIAAGLLLNRFYPRICNVIRPFLPPLSVLVASICAGAPLAFNVETMKSPLGVAILLLV 317

Query: 324 VAFHLTSFIIGYIISGFTFRDSPDAKPLQRTISFEMGMQSSLLALALANKFFEDPVVGIP 383
           VAFHL+SFI GYI+SGF FRDS D K LQRTISFE GMQSSLLALALANKFFEDP V IP
Sbjct: 318 VAFHLSSFIAGYILSGFVFRDSLDVKALQRTISFETGMQSSLLALALANKFFEDPKVAIP 377

Query: 384 SAISSPLMSLMGFSLVLIWSKGRKSKTKHST 414
            AIS+ +MSLMGF LVLIW++  K   KHS+
Sbjct: 378 PAISTSIMSLMGFVLVLIWTRRGKRDIKHSS 408


>Glyma11g03100.1 
          Length = 532

 Score =  454 bits (1168), Expect = e-128,   Method: Compositional matrix adjust.
 Identities = 240/361 (66%), Positives = 274/361 (75%), Gaps = 10/361 (2%)

Query: 31  CRTN----VTSPFSIHLRPHLPPHWSPKLPLKCAPLTSSDSLGPDQFPVHSGDLTQTLKQ 86
           CRTN    ++SPFSI      P + SPK+PLKCAPL SSDSL PD     S   TQ ++Q
Sbjct: 35  CRTNTNNNISSPFSIRFNSPFP-YRSPKIPLKCAPLHSSDSLPPDP----SSASTQ-MEQ 88

Query: 87  NWVSFLEILKQSNSLLPYVALTSTLIALVIPQSFTWFTTRHYAPALGFLMFAVGVNSKEK 146
           N +S LEILKQSNS LP+  + S L+AL+ P+S TWFT+R YAPALGFLMFAVGVNS E 
Sbjct: 89  NSMSILEILKQSNSYLPHALIASILLALIYPRSLTWFTSRFYAPALGFLMFAVGVNSNEN 148

Query: 147 DFLDAFKRPTELLTAYACQFILKPLLGYLLCIISVNVFGLPTAIGAGIVLLSCVSGAQLS 206
           DFL+AFKRP E++T Y  QF +KPLLGYL C+I+V V GLPT +GAGIVL++CVSGAQLS
Sbjct: 149 DFLEAFKRPAEIVTGYFGQFAVKPLLGYLFCMIAVTVLGLPTTVGAGIVLVACVSGAQLS 208

Query: 207 SYATFLSDPEMAPLSIVMTSLSTVSAVFVTPXXXXXXXGKRLPIDVKGMVFSITQIVLVP 266
           SYATFL+DP+MAPLSIVMTSLST SAVFVTP       GK+LPIDVKGMV++ITQIV+VP
Sbjct: 209 SYATFLTDPQMAPLSIVMTSLSTASAVFVTPLLLLLLIGKKLPIDVKGMVYNITQIVVVP 268

Query: 267 ITGGLLLNRFFPHICNSIRXXXXXXXXXXXXXXXXXXIAHNIESVKSPFGVAIFLLAVAF 326
           I  GLLLNRFFP ICN IR                  +A N+E++KSP GVAI LL VAF
Sbjct: 269 IAAGLLLNRFFPRICNVIRPFLPPLSVLVASICAGAPLALNVETMKSPLGVAILLLVVAF 328

Query: 327 HLTSFIIGYIISGFTFRDSPDAKPLQRTISFEMGMQSSLLALALANKFFEDPVVGIPSAI 386
           HL+SFI GYI+SGF FRDS D K LQRTISFE GMQSSLLALALANKFFEDP V IP AI
Sbjct: 329 HLSSFIAGYILSGFVFRDSLDVKALQRTISFETGMQSSLLALALANKFFEDPKVAIPPAI 388

Query: 387 S 387
           S
Sbjct: 389 S 389


>Glyma01g42260.2 
          Length = 326

 Score =  443 bits (1139), Expect = e-124,   Method: Compositional matrix adjust.
 Identities = 222/326 (68%), Positives = 256/326 (78%)

Query: 89  VSFLEILKQSNSLLPYVALTSTLIALVIPQSFTWFTTRHYAPALGFLMFAVGVNSKEKDF 148
           +S LEILKQSNS LP+V + S L+AL+ P S TWFT+R+YAPALGFLMFAVGVNS E DF
Sbjct: 1   MSILEILKQSNSYLPHVLIASILLALIYPPSLTWFTSRYYAPALGFLMFAVGVNSNENDF 60

Query: 149 LDAFKRPTELLTAYACQFILKPLLGYLLCIISVNVFGLPTAIGAGIVLLSCVSGAQLSSY 208
           L+AFKRP E++T Y  QF +KPLLGYL C+I+V V  LPT +GAGIVL++CVSGAQLSSY
Sbjct: 61  LEAFKRPAEIVTGYFGQFAVKPLLGYLFCMIAVTVLSLPTTVGAGIVLVACVSGAQLSSY 120

Query: 209 ATFLSDPEMAPLSIVMTSLSTVSAVFVTPXXXXXXXGKRLPIDVKGMVFSITQIVLVPIT 268
           ATFL+DP+MAPLSIVMTSLST SAVFVTP       GK+LPIDV+GMV+SITQIV+VPI 
Sbjct: 121 ATFLTDPQMAPLSIVMTSLSTASAVFVTPLLLLLLIGKKLPIDVRGMVYSITQIVVVPIA 180

Query: 269 GGLLLNRFFPHICNSIRXXXXXXXXXXXXXXXXXXIAHNIESVKSPFGVAIFLLAVAFHL 328
            GLLLNRF+P ICN IR                  +A N+E++KSP GVAI LL VAFHL
Sbjct: 181 AGLLLNRFYPRICNVIRPFLPPLSVLVASICAGAPLAFNVETMKSPLGVAILLLVVAFHL 240

Query: 329 TSFIIGYIISGFTFRDSPDAKPLQRTISFEMGMQSSLLALALANKFFEDPVVGIPSAISS 388
           +SFI GYI+SGF FRDS D K LQRTISFE GMQSSLLALALANKFFEDP V IP AIS+
Sbjct: 241 SSFIAGYILSGFVFRDSLDVKALQRTISFETGMQSSLLALALANKFFEDPKVAIPPAIST 300

Query: 389 PLMSLMGFSLVLIWSKGRKSKTKHST 414
            +MSLMGF LVLIW++  K   KHS+
Sbjct: 301 SIMSLMGFVLVLIWTRRGKRDIKHSS 326


>Glyma08g45790.1 
          Length = 417

 Score =  169 bits (429), Expect = 4e-42,   Method: Compositional matrix adjust.
 Identities = 115/308 (37%), Positives = 163/308 (52%), Gaps = 10/308 (3%)

Query: 99  NSLLPYVALTSTLIALVIPQSFTWFTTRHYAPALGFLMFAVGVNSKEKDFLDAFKRPTEL 158
           ++LLP V   + + AL  P +FTW +   YAPALG +M ++G+     DF  AFKRP  L
Sbjct: 99  SALLPVVVAATAVAALAKPSTFTWVSKELYAPALGGIMLSIGIRLSVDDFALAFKRPLPL 158

Query: 159 LTAYACQFILKPLLGYLLCIISVNVFGLPTAIGAGIVLLSCVSGAQLSSYATFLSDPEMA 218
                 Q++LKP+LG    I+    FGL     AG VL +CVSGAQLSSYA FLS  ++A
Sbjct: 159 TIGLIAQYVLKPVLG----ILVAKAFGLSRMFYAGFVLTACVSGAQLSSYANFLSKGDVA 214

Query: 219 PLSIVMTSLSTVSAVFVTPXXXXXXXGKRLPIDVKGMVFSITQIVLVPITGGLLLNRFFP 278
            L I++TS +T+++V VTP       G  +P+D   M  SI Q+VLVP+T GLLLN +  
Sbjct: 215 -LGILLTSYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVLVPVTLGLLLNTYAK 273

Query: 279 HICNSIRXXXXXXXXXXXXXXXXXXIAHNIESVKSPFGVAIFLLAVAFHLTSFIIGYIIS 338
            + + ++                  +A N   + +  G+ +    + FH+ +F +GY  S
Sbjct: 274 SVVSVLQPVMPFVAMICTSLCIGSPLALNRSQILTGEGLRLVFPVLTFHVVAFTLGYWFS 333

Query: 339 GF-TFRDSPDAKPLQRTISFEMGMQSSLLALALANKFFEDPVVGIPSAISSPLMSLMGFS 397
              + R     + + RTIS   GMQSS LA  LA +F  D    +P A S   M++MG  
Sbjct: 334 KIPSLRQE---EQVSRTISLCTGMQSSTLAGLLATQFL-DSSQAVPPACSVIAMAIMGLC 389

Query: 398 LVLIWSKG 405
           L   W  G
Sbjct: 390 LASFWGSG 397


>Glyma17g12890.1 
          Length = 429

 Score =  141 bits (356), Expect = 1e-33,   Method: Compositional matrix adjust.
 Identities = 98/333 (29%), Positives = 165/333 (49%), Gaps = 19/333 (5%)

Query: 72  QFPVHSGDLTQTLKQNWVSFLEILKQSNSLLPYVALTSTLIALVIPQSFTWFTTRHYAPA 131
             P  +G+L+Q  K      +EIL    +L P   +   ++ +  P + TW  T  ++  
Sbjct: 103 DIPESAGELSQYEK-----VIEIL---TALFPVWVILGAIVGIYKPTAVTWLATDLFSLG 154

Query: 132 LGFLMFAVGVNSKEKDFLDAFKRPTELLTAYACQFILKPLLGYLLCIISVNVFGLPTAIG 191
            GFL+ ++G+    +DF    + P  +   +  Q+++KP+LG+ + +       L   + 
Sbjct: 155 FGFLILSMGLTLTFEDFRRCLRNPWTVGVGFLAQYLIKPMLGFAIAM----TLKLSAPLA 210

Query: 192 AGIVLLSCVSGAQLSSYATFLSDPEMAPLSIVMTSLSTVSAVFVTPXXXXXXXGKRLPID 251
            G++L++C  G Q S+ ATF++   +A LS++MT+ ST+ A+ +TP       G+ +P+D
Sbjct: 211 TGLILVACCPGCQASNVATFIAKGNVA-LSVLMTTCSTIGAIIMTPLLTKLLAGQLVPVD 269

Query: 252 VKGMVFSITQIVLVPITGGLLLNRFFPHICNSIRXXXXXXXXXXXXXXXXXXIAHNIESV 311
             G+  S  Q+VLVP   G+L N  FP   + I                   I    + +
Sbjct: 270 AVGLALSTFQVVLVPTIVGVLANELFPKFTSKIITVTPLIGVILTTLLCASPIGLASDVL 329

Query: 312 KSPFGVAIFLLAVAFHLTSFIIGYIISGFTFRDSPDAKPLQRTISFEMGMQSSLLALALA 371
           K+  G  + L  V  H  SF +GY +S  +F +S       RT+S E GMQSS     LA
Sbjct: 330 KAQ-GAQLVLPVVFLHAASFALGYWVSRISFGESSS-----RTVSIECGMQSSAFGFLLA 383

Query: 372 NKFFEDPVVGIPSAISSPLMSLMGFSLVLIWSK 404
            + F +P+V +PSA+S   M+L G +L + W+ 
Sbjct: 384 QRHFTNPLVAVPSAVSVVCMALGGSALAVFWTN 416


>Glyma08g45790.2 
          Length = 315

 Score =  135 bits (341), Expect = 7e-32,   Method: Compositional matrix adjust.
 Identities = 81/178 (45%), Positives = 109/178 (61%), Gaps = 5/178 (2%)

Query: 99  NSLLPYVALTSTLIALVIPQSFTWFTTRHYAPALGFLMFAVGVNSKEKDFLDAFKRPTEL 158
           ++LLP V   + + AL  P +FTW +   YAPALG +M ++G+     DF  AFKRP  L
Sbjct: 99  SALLPVVVAATAVAALAKPSTFTWVSKELYAPALGGIMLSIGIRLSVDDFALAFKRPLPL 158

Query: 159 LTAYACQFILKPLLGYLLCIISVNVFGLPTAIGAGIVLLSCVSGAQLSSYATFLSDPEMA 218
                 Q++LKP+LG    I+    FGL     AG VL +CVSGAQLSSYA FLS  ++A
Sbjct: 159 TIGLIAQYVLKPVLG----ILVAKAFGLSRMFYAGFVLTACVSGAQLSSYANFLSKGDVA 214

Query: 219 PLSIVMTSLSTVSAVFVTPXXXXXXXGKRLPIDVKGMVFSITQIVLVPITGGLLLNRF 276
            L I++TS +T+++V VTP       G  +P+D   M  SI Q+VLVP+T GLLLN +
Sbjct: 215 -LGILLTSYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVLVPVTLGLLLNTY 271


>Glyma13g33940.1 
          Length = 415

 Score =  135 bits (339), Expect = 1e-31,   Method: Compositional matrix adjust.
 Identities = 101/376 (26%), Positives = 175/376 (46%), Gaps = 17/376 (4%)

Query: 33  TNVTSPFSIHLRPHLPPHWSPKLPLKCAPLTSSDSLGPDQFPVHSGDLTQTLKQNWVSFL 92
           T + S + I LR +  P   P L    +P  S  ++ P +  + S + +    ++   +L
Sbjct: 49  TQIKSTYRITLRCNSQPQPLPNLFASKSPRVSGSNVAPLRCGISSNEYSANEGRSVGEWL 108

Query: 93  EILKQSNSL-LPYVALTSTLIALVIPQSFTWFTTRHYAPALGFLMFAVGVNSKEKDFLDA 151
            +  +  S   P     ++++ L+ P  F W T +     L  +M  +G+     D    
Sbjct: 109 VLAGEVLSTGFPLWVTIASVLGLMKPSYFNWVTPKLSIMGLNIIMLGMGMTLTLDDLRGV 168

Query: 152 FKRPTELLTAYACQFILKPLLGYLLCIISVNVFGLPTAIGAGIVLLSCVSGAQLSSYATF 211
              P E+L+ +  Q+ + PL G+L+      +  LP+   AGI+LL C  G   S+  T+
Sbjct: 169 LAMPKEVLSGFLIQYSVMPLSGFLIS----KLLNLPSHSAAGIILLGCCPGGTASNIITY 224

Query: 212 LSDPEMAPLSIVMTSLSTVSAVFVTPXXXXXXXGKRLPIDVKGMVFSITQIVLVPITGGL 271
           L+   +A LS++MT+ ST+SA+ +TP       GK + +D  G++ S  Q+VLVP+  G 
Sbjct: 225 LARANVA-LSVIMTTASTISAMIMTPLLTAKLAGKYVAVDAPGLLISTLQVVLVPVLAGA 283

Query: 272 LLNRFFPHICNSIRXXXXXXXXXXXXXXXXXXIAHNIESVKSPFG---VAIFLLAVAFHL 328
            LN+FF  I   +                   I  +  ++ +  G   +A FLL      
Sbjct: 284 FLNQFFQPIVKFVSPLMPPIAVITVAILCGNAIGQSSSAILASGGQVILATFLL----QA 339

Query: 329 TSFIIGYIISGFTFRDSPDAKPLQRTISFEMGMQSSLLALALANKFFEDPVVGIPSAISS 388
           + F  GY+ +     D   +    RTIS ++GM++ +L + LA K F DP+  +P A++ 
Sbjct: 340 SGFFFGYLFARLLRLDVSSS----RTISSQVGMKNCVLQIVLATKHFGDPLSAVPGALAI 395

Query: 389 PLMSLMGFSLVLIWSK 404
              S++G  L  IW +
Sbjct: 396 VNQSIIGSILAGIWRR 411


>Glyma05g08120.1 
          Length = 382

 Score =  132 bits (333), Expect = 5e-31,   Method: Compositional matrix adjust.
 Identities = 91/291 (31%), Positives = 147/291 (50%), Gaps = 16/291 (5%)

Query: 121 TWFTTRHYAPALGFLMFAVGVNSKEKDFLDAFKRPTELLTAYACQFILKPLLGYLLCI-- 178
           TW  T  ++  LGFLM ++G+    +DF    + P  +   +  Q+++KP+LG+ + +  
Sbjct: 84  TWLATDLFSLGLGFLMLSMGLTLTFEDFRRCLQNPWTVGIGFLAQYLIKPMLGFAIAMQA 143

Query: 179 -------ISVNVFGLPTAIGAGIVLLSCVSGAQLSSYATFLSDPEMAPLSIVMTSLSTVS 231
                    V ++     +  G++L++C  G Q S+ ATF++   +A LS++MT+ ST+ 
Sbjct: 144 ETKAMLYFQVILYLRQAPLATGLILVACCPGCQASNVATFIAKGNVA-LSVLMTTCSTIG 202

Query: 232 AVFVTPXXXXXXXGKRLPIDVKGMVFSITQIVLVPITGGLLLNRFFPHICNSIRXXXXXX 291
           A+ +TP       G+ +P+D  G+  S  Q+VLVP   G+L N  FP   + I       
Sbjct: 203 AIIMTPLLTNLLAGQLVPVDAVGLALSTFQVVLVPTIVGVLANELFPKFTSKIITITPLI 262

Query: 292 XXXXXXXXXXXXIAHNIESVKSPFGVAIFLLAVAFHLTSFIIGYIISGFTFRDSPDAKPL 351
                       I    +++K+  G  + L  V  H  SF +GY +S  +F +S      
Sbjct: 263 GVILTTLLCASPIGLASDALKAQ-GAQLVLPVVFLHAASFALGYWVSRISFGESTS---- 317

Query: 352 QRTISFEMGMQSSLLALALANKFFEDPVVGIPSAISSPLMSLMGFSLVLIW 402
            RTIS E GMQSS     LA K F +P+V +PSA+S   M+L G +L + W
Sbjct: 318 -RTISIECGMQSSAFGFLLAQKHFTNPLVAVPSAVSVVCMALGGSALAVFW 367


>Glyma12g36310.1 
          Length = 366

 Score =  124 bits (310), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 91/331 (27%), Positives = 152/331 (45%), Gaps = 19/331 (5%)

Query: 75  VHSGDLTQTLKQNWVSFLEILKQSNSLLPYVALTSTLIALVIPQSFTWFTTRHYAPALGF 134
           ++ G+  +++++  V   E L  +    P       ++ L+ P  F W T +     L  
Sbjct: 44  MYGGNEVRSVREWLVLAGEFLSMA---FPLWVSIGCVLGLMKPNCFNWVTPKVTIRGLNI 100

Query: 135 LMFAVGVNSKEKDFLDAFKRPTELLTAYACQFILKPLLGYLLCIISVNVFGLPTAIGAGI 194
           +M  +G+     D   A   P ++L  +  Q+ + PL G+L+      +  L     AG+
Sbjct: 101 IMLGMGMTLTLDDLRGALAMPKQVLYGFVLQYSVMPLSGFLIS----TLLNLAPHFAAGL 156

Query: 195 VLLSCVSGAQLSSYATFLSDPEMAPLSIVMTSLSTVSAVFVTPXXXXXXXGKRLPIDVKG 254
           +L+ C  G   S+  T+LS   +A LS++MT+ ST++A  +TP       GK + +D  G
Sbjct: 157 ILIGCSPGGTASNIITYLSRGNVA-LSVIMTTASTLTATIMTPFLTATLAGKYVAVDASG 215

Query: 255 MVFSITQIVLVPITGGLLLNRFFPHICNSIRXXXXXXXXXXXXXXXXXXIAHNIESVKSP 314
           ++ S  Q+VL P+  G  LN+FF      +                   IA +  ++   
Sbjct: 216 LLISTLQVVLFPVLAGAFLNQFFKPFVKLVSPLMPPIAVTTVAILCGNSIAQSSSAILVC 275

Query: 315 FG---VAIFLLAVAFHLTSFIIGYIISGFTFRDSPDAKPLQRTISFEMGMQSSLLALALA 371
            G   +A FLL    H + F  GYI       D   +    RTIS ++GM++S+L + LA
Sbjct: 276 GGQVILATFLL----HASGFFFGYIFGRLLGLDVSSS----RTISTQVGMKNSVLGIVLA 327

Query: 372 NKFFEDPVVGIPSAISSPLMSLMGFSLVLIW 402
            K F DP+  +P A+S    S++G  L  IW
Sbjct: 328 TKHFGDPLTAVPGAVSIVFQSIIGSILAGIW 358