Miyakogusa Predicted Gene
- Lj2g3v0523450.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj2g3v0523450.1 tr|G7KHS6|G7KHS6_MEDTR 4,5-DOPA dioxygenase
extradiol-like protein OS=Medicago truncatula
GN=MTR_6g0,72.41,1e-16,LigB,Extradiol ring-cleavage dioxygenase, class
III enzyme, subunit B; LigB subunit of an aromatic-r,CUFF.34670.1
(267 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma16g33280.1 489 e-138
Glyma09g28490.1 408 e-114
Glyma16g33270.1 395 e-110
Glyma20g35700.1 332 3e-91
Glyma09g28500.1 295 2e-80
Glyma16g33290.1 74 2e-13
>Glyma16g33280.1
Length = 266
Score = 489 bits (1258), Expect = e-138, Method: Compositional matrix adjust.
Identities = 234/264 (88%), Positives = 251/264 (95%), Gaps = 1/264 (0%)
Query: 1 MALKDTFYISHGSPTLSIDESLVARKFLQSWKKEVFPPRPTSILVISGHWDTAVPTVNVV 60
MALKDTFYISHGSPTLSIDES+ ARKFLQSWKK+VFP RP+SILVISGHW+TAVPTVNVV
Sbjct: 1 MALKDTFYISHGSPTLSIDESIQARKFLQSWKKDVFPQRPSSILVISGHWETAVPTVNVV 60
Query: 61 DSTNDTIYDFYGFPKPMYQLKYPAPGAPHLAKRVKELLKEGGFSRVDEDKKRGLDHGAWV 120
DS NDTIYDFYGFPK MYQLKYPAPGAP LA+RVKELLK+ GFS VDED KRGLDHGAWV
Sbjct: 61 DSINDTIYDFYGFPKQMYQLKYPAPGAPQLARRVKELLKKSGFSHVDEDTKRGLDHGAWV 120
Query: 121 PLLLMYPEADIPVCQLSVQSNLDGTHHYNIGKALAPLKDEGVLIVGSGSAVHNLRALERH 180
PL LMYPEADIPVCQ+S+QS DGT+HYN+GKALAPLKDEGVLI+GSGSAVHNLRALE H
Sbjct: 121 PLFLMYPEADIPVCQISIQSQQDGTYHYNLGKALAPLKDEGVLIMGSGSAVHNLRALEPH 180
Query: 181 ATVAAPWAVEFDNWLKEALLEGRYEDVNHYEQKAPHAKKAHPWPDHFYPLHVAIGAAGEN 240
+TV APWA+EFDNWLK+ALLEGRYEDVNHYEQKAPHAKKAHPWPDHFYPLHVAIGAAGE+
Sbjct: 181 STV-APWALEFDNWLKDALLEGRYEDVNHYEQKAPHAKKAHPWPDHFYPLHVAIGAAGED 239
Query: 241 SKAKLIHSSIDLGSLSYASYQFTS 264
+KAKLIHSSI+LGSLSYASYQFTS
Sbjct: 240 AKAKLIHSSIELGSLSYASYQFTS 263
>Glyma09g28490.1
Length = 303
Score = 408 bits (1049), Expect = e-114, Method: Compositional matrix adjust.
Identities = 197/264 (74%), Positives = 225/264 (85%), Gaps = 2/264 (0%)
Query: 1 MALKDTFYISHGSPTLSIDESLVARKFLQSWKKEVFPPRPTSILVISGHWDTAVPTVNVV 60
+ LK+TFY+SHG+PTL++D+S+ A F SWK E FP RP+SIL+IS HWDT VPTVNVV
Sbjct: 39 LRLKETFYLSHGAPTLAVDDSIPAWNFFNSWK-EQFPTRPSSILIISAHWDTHVPTVNVV 97
Query: 61 DSTNDTIYDFYGFPKPMYQLKYPAPGAPHLAKRVKELLKEGGFSRVDEDKKRGLDHGAWV 120
NDTIYDFYGFPK MY+LKYPAPGAPHLAKRVKELL GFS VDEDKKRGLDHGAWV
Sbjct: 98 HQ-NDTIYDFYGFPKSMYKLKYPAPGAPHLAKRVKELLLGSGFSHVDEDKKRGLDHGAWV 156
Query: 121 PLLLMYPEADIPVCQLSVQSNLDGTHHYNIGKALAPLKDEGVLIVGSGSAVHNLRALERH 180
PLLLMYPEADIPVCQLS+ SN GT+HYN+GKALAPLKDEGVLIVGSGSA HNLRA+
Sbjct: 157 PLLLMYPEADIPVCQLSISSNKGGTYHYNMGKALAPLKDEGVLIVGSGSATHNLRAIAPR 216
Query: 181 ATVAAPWAVEFDNWLKEALLEGRYEDVNHYEQKAPHAKKAHPWPDHFYPLHVAIGAAGEN 240
T APWA F +WLK +LL+GRYE+VN YE+KAP+AK AHPWPDHF+PLHVA+GAAGEN
Sbjct: 217 GTPPAPWASAFMSWLKTSLLDGRYEEVNEYEEKAPYAKMAHPWPDHFFPLHVAMGAAGEN 276
Query: 241 SKAKLIHSSIDLGSLSYASYQFTS 264
SKAK++H S D GS+SYAS+ FT+
Sbjct: 277 SKAKVVHDSWDGGSMSYASFGFTT 300
>Glyma16g33270.1
Length = 269
Score = 395 bits (1016), Expect = e-110, Method: Compositional matrix adjust.
Identities = 190/264 (71%), Positives = 222/264 (84%), Gaps = 2/264 (0%)
Query: 1 MALKDTFYISHGSPTLSIDESLVARKFLQSWKKEVFPPRPTSILVISGHWDTAVPTVNVV 60
+ LK+TFY+SHG+P+L ID+S+ A F SWK E FP +P+SILVIS HWDT VPTVNVV
Sbjct: 5 LKLKETFYLSHGAPSLVIDDSIPAWHFFNSWK-EQFPTKPSSILVISAHWDTHVPTVNVV 63
Query: 61 DSTNDTIYDFYGFPKPMYQLKYPAPGAPHLAKRVKELLKEGGFSRVDEDKKRGLDHGAWV 120
D NDTIYDF GFPK MY+LKYPAPGAP LAKRVKELL GFS VDEDKKRGLDHGAWV
Sbjct: 64 DQ-NDTIYDFSGFPKSMYKLKYPAPGAPQLAKRVKELLLGSGFSHVDEDKKRGLDHGAWV 122
Query: 121 PLLLMYPEADIPVCQLSVQSNLDGTHHYNIGKALAPLKDEGVLIVGSGSAVHNLRALERH 180
PL LMYPEADIPVCQLS+ SN GT+HYN+GKALAPLKDEGVLI+GSGSA HNLRA+
Sbjct: 123 PLFLMYPEADIPVCQLSISSNKGGTYHYNMGKALAPLKDEGVLIIGSGSATHNLRAMAPR 182
Query: 181 ATVAAPWAVEFDNWLKEALLEGRYEDVNHYEQKAPHAKKAHPWPDHFYPLHVAIGAAGEN 240
+ APWA F +WL+ +LL+GRYE+VN +E+KAP+AK AHPWPDHF+PLHVA+GAAGEN
Sbjct: 183 GSPPAPWASAFMSWLETSLLDGRYEEVNEFEEKAPYAKMAHPWPDHFFPLHVAMGAAGEN 242
Query: 241 SKAKLIHSSIDLGSLSYASYQFTS 264
+KAK++H S D GS+SYAS+ FT+
Sbjct: 243 AKAKVVHDSWDAGSISYASFGFTT 266
>Glyma20g35700.1
Length = 250
Score = 332 bits (850), Expect = 3e-91, Method: Compositional matrix adjust.
Identities = 169/266 (63%), Positives = 207/266 (77%), Gaps = 21/266 (7%)
Query: 1 MALKDTFYISHGSPTLSIDESLVARKFLQSWKKEVFPPRPTSILVISGHWDTAVPTVNVV 60
MALKDTF+ISHGS T+SID+S+ A +FL SWK EV P R +SILVISGHWDT VPTVNVV
Sbjct: 1 MALKDTFFISHGSSTISIDDSIPAWEFLTSWK-EVLPQRFSSILVISGHWDTDVPTVNVV 59
Query: 61 DSTNDTIYDFYGFPKPMYQLKYPAP--GAPHLAKRVKELLKEGGFSRVDEDKKRGLDHGA 118
D N TIYD+YGFPK MY+ + GAPH+AKRVKELL+ GFS+VDED+K G DHGA
Sbjct: 60 DH-NKTIYDYYGFPKVMYKKTKVSSTRGAPHVAKRVKELLEASGFSKVDEDRKGGHDHGA 118
Query: 119 WVPLLLMYPEADIPVCQLSVQSNLDGTHHYNIGKALAPLKDEGVLIVGSGSAVHNLRALE 178
WVPL+L +DGT+HYN+GK LAPLKD+ VLI+ SGSA+HNLRA+
Sbjct: 119 WVPLML-----------------IDGTYHYNLGKVLAPLKDQDVLIIRSGSALHNLRAIG 161
Query: 179 RHATVAAPWAVEFDNWLKEALLEGRYEDVNHYEQKAPHAKKAHPWPDHFYPLHVAIGAAG 238
+ APWA+ FD+WLK +L+EGRYE+VN +++KAP+AK AHPWPDHF+PLHVA+GAAG
Sbjct: 162 PRNSPTAPWALAFDSWLKNSLIEGRYEEVNKFDEKAPYAKLAHPWPDHFFPLHVAMGAAG 221
Query: 239 ENSKAKLIHSSIDLGSLSYASYQFTS 264
ENSKAK++H S D GS+S AS+ FT+
Sbjct: 222 ENSKAKIVHHSWDAGSISCASFGFTA 247
>Glyma09g28500.1
Length = 271
Score = 295 bits (756), Expect = 2e-80, Method: Compositional matrix adjust.
Identities = 167/289 (57%), Positives = 190/289 (65%), Gaps = 46/289 (15%)
Query: 1 MALKDTFYISHGSPTLSIDESLVARKFLQSWKKEVFPPRP----TSILVISGHWD----- 51
M LK T YISHGSPTLSIDE + ARKFLQSWKK+VFP P +S+L ++ +
Sbjct: 1 MMLKITLYISHGSPTLSIDEYIDARKFLQSWKKDVFPQNPFQYLSSLLTVTLQFHPSTLL 60
Query: 52 TAVPTVNVVDSTNDTIYDFYGFPKPMYQLKYPAPGAPHLAKRVKELLKEGGFSRVDEDKK 111
T T +++ T+ Y + + Y KYPAPGAP LA+RVKELL + GFSRVDED K
Sbjct: 61 TPSTTPSMISITSPNKCTRYIYIQISYIHKYPAPGAPQLARRVKELLIKSGFSRVDEDTK 120
Query: 112 RGLDHGAWVPLLLMYPEADIPVCQLSVQSNLDGTHHYNIGKALAPLKDEGVLIVGSGSAV 171
GLDHGA VPL LMYPEADIPVCQLSVQS DGT+HYN GKALAPLKDE VLI+GSG
Sbjct: 121 PGLDHGARVPLFLMYPEADIPVCQLSVQSQQDGTYHYNFGKALAPLKDESVLIIGSG--- 177
Query: 172 HNLRALERHATVAAPWAVEFDNWLKEALLEGR------------YED----VNHYEQKAP 215
+F NWLK+ALLEGR Y D V +
Sbjct: 178 ------------------KFYNWLKDALLEGRELFRSSLIGLKDYCDSGMMVYCIDLITI 219
Query: 216 HAKKAHPWPDHFYPLHVAIGAAGENSKAKLIHSSIDLGSLSYASYQFTS 264
+ AHPWPDHFYPLHVAIGAAGEN+K KLIHSSIDLG+LSYASYQFTS
Sbjct: 220 YQDTAHPWPDHFYPLHVAIGAAGENAKVKLIHSSIDLGTLSYASYQFTS 268
>Glyma16g33290.1
Length = 42
Score = 73.6 bits (179), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 35/42 (83%), Positives = 40/42 (95%), Gaps = 1/42 (2%)
Query: 220 AHPWPDHFY-PLHVAIGAAGENSKAKLIHSSIDLGSLSYASY 260
AHPWPDHFY PLHVAIGAAGEN+KAKLIH+SI++G+LSYA Y
Sbjct: 1 AHPWPDHFYHPLHVAIGAAGENAKAKLIHNSIEMGTLSYAFY 42