Miyakogusa Predicted Gene
- Lj2g3v0523330.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj2g3v0523330.1 Non Chatacterized Hit- tr|I3SYT8|I3SYT8_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,99.34,0,seg,NULL;
LigB subunit of an aromatic-ring-opening dioxygenase LigAB,Extradiol
ring-cleavage dioxyge,CUFF.34701.1
(304 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma09g28490.1 493 e-139
Glyma16g33270.1 468 e-132
Glyma16g33280.1 415 e-116
Glyma20g35700.1 380 e-106
Glyma09g28500.1 260 2e-69
Glyma16g33290.1 64 2e-10
>Glyma09g28490.1
Length = 303
Score = 493 bits (1269), Expect = e-139, Method: Compositional matrix adjust.
Identities = 236/304 (77%), Positives = 260/304 (85%), Gaps = 2/304 (0%)
Query: 1 MNSIFRFCHSSANSKPRXXXXXXXXXXXXXNINNNSI-MALKETFYISHGSPTLAIDDSI 59
MN IFRFC+S +N NI ++ + LKETFY+SHG+PTLA+DDSI
Sbjct: 1 MNKIFRFCYSFSNYSVARREEKIIESGDSSNIEERNMGLRLKETFYLSHGAPTLAVDDSI 60
Query: 60 PAWKFLNSWKEEVFPHRPSSILIISGHWDTHVPTVNAVDRNDTIYDFYGFPKNMYKLKYP 119
PAW F NSWKE+ FP RPSSILIIS HWDTHVPTVN V +NDTIYDFYGFPK+MYKLKYP
Sbjct: 61 PAWNFFNSWKEQ-FPTRPSSILIISAHWDTHVPTVNVVHQNDTIYDFYGFPKSMYKLKYP 119
Query: 120 APGAPHLAKRVKELLTASGFSRVDEDRKRGLDHGAWVPLMLMYPDADIPVCQLSVSSSMG 179
APGAPHLAKRVKELL SGFS VDED+KRGLDHGAWVPL+LMYP+ADIPVCQLS+SS+ G
Sbjct: 120 APGAPHLAKRVKELLLGSGFSHVDEDKKRGLDHGAWVPLLLMYPEADIPVCQLSISSNKG 179
Query: 180 GTYHYEMGKALAPLKDEGVLIIGSGSATHNLRAIGPRNSPPPPWAVDFMSWLKSSLLHGR 239
GTYHY MGKALAPLKDEGVLI+GSGSATHNLRAI PR +PP PWA FMSWLK+SLL GR
Sbjct: 180 GTYHYNMGKALAPLKDEGVLIVGSGSATHNLRAIAPRGTPPAPWASAFMSWLKTSLLDGR 239
Query: 240 YEEVNQYEEKAPYAKMAHPWPDHFFPLHVAMGAAGENAKAKVVHDSWDGGSISYASFGFT 299
YEEVN+YEEKAPYAKMAHPWPDHFFPLHVAMGAAGEN+KAKVVHDSWDGGS+SYASFGFT
Sbjct: 240 YEEVNEYEEKAPYAKMAHPWPDHFFPLHVAMGAAGENSKAKVVHDSWDGGSMSYASFGFT 299
Query: 300 ADNT 303
++
Sbjct: 300 TADS 303
>Glyma16g33270.1
Length = 269
Score = 468 bits (1203), Expect = e-132, Method: Compositional matrix adjust.
Identities = 222/265 (83%), Positives = 240/265 (90%), Gaps = 1/265 (0%)
Query: 35 NSIMALKETFYISHGSPTLAIDDSIPAWKFLNSWKEEVFPHRPSSILIISGHWDTHVPTV 94
N + LKETFY+SHG+P+L IDDSIPAW F NSWKE+ FP +PSSIL+IS HWDTHVPTV
Sbjct: 2 NMGLKLKETFYLSHGAPSLVIDDSIPAWHFFNSWKEQ-FPTKPSSILVISAHWDTHVPTV 60
Query: 95 NAVDRNDTIYDFYGFPKNMYKLKYPAPGAPHLAKRVKELLTASGFSRVDEDRKRGLDHGA 154
N VD+NDTIYDF GFPK+MYKLKYPAPGAP LAKRVKELL SGFS VDED+KRGLDHGA
Sbjct: 61 NVVDQNDTIYDFSGFPKSMYKLKYPAPGAPQLAKRVKELLLGSGFSHVDEDKKRGLDHGA 120
Query: 155 WVPLMLMYPDADIPVCQLSVSSSMGGTYHYEMGKALAPLKDEGVLIIGSGSATHNLRAIG 214
WVPL LMYP+ADIPVCQLS+SS+ GGTYHY MGKALAPLKDEGVLIIGSGSATHNLRA+
Sbjct: 121 WVPLFLMYPEADIPVCQLSISSNKGGTYHYNMGKALAPLKDEGVLIIGSGSATHNLRAMA 180
Query: 215 PRNSPPPPWAVDFMSWLKSSLLHGRYEEVNQYEEKAPYAKMAHPWPDHFFPLHVAMGAAG 274
PR SPP PWA FMSWL++SLL GRYEEVN++EEKAPYAKMAHPWPDHFFPLHVAMGAAG
Sbjct: 181 PRGSPPAPWASAFMSWLETSLLDGRYEEVNEFEEKAPYAKMAHPWPDHFFPLHVAMGAAG 240
Query: 275 ENAKAKVVHDSWDGGSISYASFGFT 299
ENAKAKVVHDSWD GSISYASFGFT
Sbjct: 241 ENAKAKVVHDSWDAGSISYASFGFT 265
>Glyma16g33280.1
Length = 266
Score = 415 bits (1067), Expect = e-116, Method: Compositional matrix adjust.
Identities = 197/264 (74%), Positives = 229/264 (86%), Gaps = 2/264 (0%)
Query: 38 MALKETFYISHGSPTLAIDDSIPAWKFLNSWKEEVFPHRPSSILIISGHWDTHVPTVNAV 97
MALK+TFYISHGSPTL+ID+SI A KFL SWK++VFP RPSSIL+ISGHW+T VPTVN V
Sbjct: 1 MALKDTFYISHGSPTLSIDESIQARKFLQSWKKDVFPQRPSSILVISGHWETAVPTVNVV 60
Query: 98 DR-NDTIYDFYGFPKNMYKLKYPAPGAPHLAKRVKELLTASGFSRVDEDRKRGLDHGAWV 156
D NDTIYDFYGFPK MY+LKYPAPGAP LA+RVKELL SGFS VDED KRGLDHGAWV
Sbjct: 61 DSINDTIYDFYGFPKQMYQLKYPAPGAPQLARRVKELLKKSGFSHVDEDTKRGLDHGAWV 120
Query: 157 PLMLMYPDADIPVCQLSVSSSMGGTYHYEMGKALAPLKDEGVLIIGSGSATHNLRAIGPR 216
PL LMYP+ADIPVCQ+S+ S GTYHY +GKALAPLKDEGVLI+GSGSA HNLRA+ P
Sbjct: 121 PLFLMYPEADIPVCQISIQSQQDGTYHYNLGKALAPLKDEGVLIMGSGSAVHNLRALEP- 179
Query: 217 NSPPPPWAVDFMSWLKSSLLHGRYEEVNQYEEKAPYAKMAHPWPDHFFPLHVAMGAAGEN 276
+S PWA++F +WLK +LL GRYE+VN YE+KAP+AK AHPWPDHF+PLHVA+GAAGE+
Sbjct: 180 HSTVAPWALEFDNWLKDALLEGRYEDVNHYEQKAPHAKKAHPWPDHFYPLHVAIGAAGED 239
Query: 277 AKAKVVHDSWDGGSISYASFGFTA 300
AKAK++H S + GS+SYAS+ FT+
Sbjct: 240 AKAKLIHSSIELGSLSYASYQFTS 263
>Glyma20g35700.1
Length = 250
Score = 380 bits (977), Expect = e-106, Method: Compositional matrix adjust.
Identities = 191/265 (72%), Positives = 213/265 (80%), Gaps = 20/265 (7%)
Query: 38 MALKETFYISHGSPTLAIDDSIPAWKFLNSWKEEVFPHRPSSILIISGHWDTHVPTVNAV 97
MALK+TF+ISHGS T++IDDSIPAW+FL SWKE V P R SSIL+ISGHWDT VPTVN V
Sbjct: 1 MALKDTFFISHGSSTISIDDSIPAWEFLTSWKE-VLPQRFSSILVISGHWDTDVPTVNVV 59
Query: 98 DRNDTIYDFYGFPKNMYKLKYPAP--GAPHLAKRVKELLTASGFSRVDEDRKRGLDHGAW 155
D N TIYD+YGFPK MYK + GAPH+AKRVKELL ASGFS+VDEDRK G DHGAW
Sbjct: 60 DHNKTIYDYYGFPKVMYKKTKVSSTRGAPHVAKRVKELLEASGFSKVDEDRKGGHDHGAW 119
Query: 156 VPLMLMYPDADIPVCQLSVSSSMGGTYHYEMGKALAPLKDEGVLIIGSGSATHNLRAIGP 215
VPLML+ GTYHY +GK LAPLKD+ VLII SGSA HNLRAIGP
Sbjct: 120 VPLMLI-----------------DGTYHYNLGKVLAPLKDQDVLIIRSGSALHNLRAIGP 162
Query: 216 RNSPPPPWAVDFMSWLKSSLLHGRYEEVNQYEEKAPYAKMAHPWPDHFFPLHVAMGAAGE 275
RNSP PWA+ F SWLK+SL+ GRYEEVN+++EKAPYAK+AHPWPDHFFPLHVAMGAAGE
Sbjct: 163 RNSPTAPWALAFDSWLKNSLIEGRYEEVNKFDEKAPYAKLAHPWPDHFFPLHVAMGAAGE 222
Query: 276 NAKAKVVHDSWDGGSISYASFGFTA 300
N+KAK+VH SWD GSIS ASFGFTA
Sbjct: 223 NSKAKIVHHSWDAGSISCASFGFTA 247
>Glyma09g28500.1
Length = 271
Score = 260 bits (664), Expect = 2e-69, Method: Compositional matrix adjust.
Identities = 148/290 (51%), Positives = 178/290 (61%), Gaps = 49/290 (16%)
Query: 38 MALKETFYISHGSPTLAIDDSIPAWKFLNSWKEEVFPHRP----SSILIISGHWD----- 88
M LK T YISHGSPTL+ID+ I A KFL SWK++VFP P SS+L ++ +
Sbjct: 1 MMLKITLYISHGSPTLSIDEYIDARKFLQSWKKDVFPQNPFQYLSSLLTVTLQFHPSTLL 60
Query: 89 --THVPTVNAVDRNDTIYDFYGFPKNMYKLKYPAPGAPHLAKRVKELLTASGFSRVDEDR 146
+ P++ ++ + Y + + Y KYPAPGAP LA+RVKELL SGFSRVDED
Sbjct: 61 TPSTTPSMISITSPNKCTR-YIYIQISYIHKYPAPGAPQLARRVKELLIKSGFSRVDEDT 119
Query: 147 KRGLDHGAWVPLMLMYPDADIPVCQLSVSSSMGGTYHYEMGKALAPLKDEGVLIIGSGSA 206
K GLDHGA VPL LMYP+ADIPVCQLSV S GTYHY GKALAPLKDE VLIIGSG
Sbjct: 120 KPGLDHGARVPLFLMYPEADIPVCQLSVQSQQDGTYHYNFGKALAPLKDESVLIIGSGK- 178
Query: 207 THNLRAIGPRNSPPPPWAVDFMSWLKSSLLHGR------------YEE----VNQYEEKA 250
F +WLK +LL GR Y + V +
Sbjct: 179 --------------------FYNWLKDALLEGRELFRSSLIGLKDYCDSGMMVYCIDLIT 218
Query: 251 PYAKMAHPWPDHFFPLHVAMGAAGENAKAKVVHDSWDGGSISYASFGFTA 300
Y AHPWPDHF+PLHVA+GAAGENAK K++H S D G++SYAS+ FT+
Sbjct: 219 IYQDTAHPWPDHFYPLHVAIGAAGENAKVKLIHSSIDLGTLSYASYQFTS 268
>Glyma16g33290.1
Length = 42
Score = 63.5 bits (153), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 29/42 (69%), Positives = 38/42 (90%), Gaps = 1/42 (2%)
Query: 256 AHPWPDHFF-PLHVAMGAAGENAKAKVVHDSWDGGSISYASF 296
AHPWPDHF+ PLHVA+GAAGENAKAK++H+S + G++SYA +
Sbjct: 1 AHPWPDHFYHPLHVAIGAAGENAKAKLIHNSIEMGTLSYAFY 42