Miyakogusa Predicted Gene
- Lj1g3v5061070.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj1g3v5061070.1 Non Chatacterized Hit- tr|J9JB62|J9JB62_9SPIT
Membrane protein, putative OS=Oxytricha trifallax
PE=4,33.49,9e-19,S5A_REDUCTASE,3-oxo-5-alpha-steroid 4-dehydrogenase,
C-terminal; DUF1295,Protein of unknown
function,NODE_31073_length_1833_cov_57.566830.path1.1
(332 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma19g45120.1 502 e-142
Glyma16g01850.1 496 e-140
Glyma07g05300.1 493 e-139
Glyma19g45120.2 428 e-120
Glyma05g33940.3 81 1e-15
Glyma05g33940.1 81 2e-15
Glyma05g33940.2 77 2e-14
Glyma08g05750.1 74 2e-13
>Glyma19g45120.1
Length = 317
Score = 502 bits (1292), Expect = e-142, Method: Compositional matrix adjust.
Identities = 245/332 (73%), Positives = 275/332 (82%), Gaps = 15/332 (4%)
Query: 1 MGSGTAVSNMKNAILAFLVPLPSILFYLSFLSATNGSVADPKSPSFTSTVWTWCYHYPXX 60
MGS + SN+KNA++AFLVPLPSILFYLSF SA + +PS S++WTWCYH+P
Sbjct: 1 MGS-VSYSNLKNAMIAFLVPLPSILFYLSFQSAIS-------TPSSWSSLWTWCYHHPLL 52
Query: 61 XXXXXXXXXXXXXXWVIGLIQSSHWMIDPYWTVIPVMLVHYYSAHPLAQYHCWRSRIVIL 120
W+IGLIQSSHWMIDPYWTVIPVMLVHYYS HPLAQY WRSRIV L
Sbjct: 53 LANVLFFFNVNFLFWLIGLIQSSHWMIDPYWTVIPVMLVHYYSTHPLAQYQWWRSRIVTL 112
Query: 121 LTWVWSIRLIHNYFRREKWQWGVREDWRFTDMSHQYGSHWWWVSFFSIYVPQQLFLMGLS 180
LTW WS+RLIHNYFRREKWQWG REDWRFTD+SH+YG HWWW SFF+IYVPQQ+FL+GLS
Sbjct: 113 LTWAWSVRLIHNYFRREKWQWGAREDWRFTDLSHRYGRHWWWASFFAIYVPQQVFLIGLS 172
Query: 181 LPFYVIHSVNQPLSIWDLLATIVCVSGIVIAYFADTQLHNFVSRNNKLKGQGKSVVPVLD 240
LPFYVIHSVNQPLS+WDL+A +VCVSGIV AY ADTQL+NFVSR NK VP+LD
Sbjct: 173 LPFYVIHSVNQPLSMWDLVAIVVCVSGIVTAYIADTQLYNFVSRKNK-------EVPILD 225
Query: 241 NGLWYYSRHPNYFGEQLWWWGLVVFTWNLGHGWTVIGALANTMCLAYVTKLVENRMLSQD 300
GLWYYSRHPNYFGEQ+WWWG+ VF WNLGHGW IGALANTMCLAYVTKLVE+RML QD
Sbjct: 226 KGLWYYSRHPNYFGEQVWWWGMAVFAWNLGHGWAFIGALANTMCLAYVTKLVEDRMLKQD 285
Query: 301 NRAEAYRLYQRTTSVWVPWFKSSPLGLKSKNV 332
+RAEA+RLYQ+TTS+W+PWFKSSPLGLKSK+
Sbjct: 286 SRAEAFRLYQKTTSLWIPWFKSSPLGLKSKDA 317
>Glyma16g01850.1
Length = 325
Score = 496 bits (1276), Expect = e-140, Method: Compositional matrix adjust.
Identities = 236/331 (71%), Positives = 274/331 (82%), Gaps = 7/331 (2%)
Query: 1 MGSGTAVSNMKNAILAFLVPLPSILFYLSFLSATNGSVADPKSPSFTSTVWTWCYHYPXX 60
M SG N+KNA++AFLVPLPSI FYLSFL+ + + KSPSF ST+W+WCYH+P
Sbjct: 1 MASG---RNLKNAVIAFLVPLPSIFFYLSFLNHYDSA----KSPSFWSTLWSWCYHHPLL 53
Query: 61 XXXXXXXXXXXXXXWVIGLIQSSHWMIDPYWTVIPVMLVHYYSAHPLAQYHCWRSRIVIL 120
W+IGLIQSSHWMIDPYWTVIPVMLVHYY+ HPLA Y WRS+IVIL
Sbjct: 54 LANALFFLNVNVLFWLIGLIQSSHWMIDPYWTVIPVMLVHYYATHPLAPYDLWRSKIVIL 113
Query: 121 LTWVWSIRLIHNYFRREKWQWGVREDWRFTDMSHQYGSHWWWVSFFSIYVPQQLFLMGLS 180
LTWVWS+RL HNYFRRE+WQWG REDWRFT+MS QYG WWWVSFF++YV QQ+FL+ LS
Sbjct: 114 LTWVWSVRLTHNYFRRERWQWGAREDWRFTEMSQQYGKQWWWVSFFAVYVSQQMFLIALS 173
Query: 181 LPFYVIHSVNQPLSIWDLLATIVCVSGIVIAYFADTQLHNFVSRNNKLKGQGKSVVPVLD 240
LP Y +H+VNQPL++WDL+AT+VC+ GIVIAYFADTQL+ FVSRNNKLKG GK VV VLD
Sbjct: 174 LPLYAVHTVNQPLNMWDLVATVVCLCGIVIAYFADTQLYEFVSRNNKLKGLGKPVVSVLD 233
Query: 241 NGLWYYSRHPNYFGEQLWWWGLVVFTWNLGHGWTVIGALANTMCLAYVTKLVENRMLSQD 300
+GLWYY RHPNYFGEQLWWWGLVVF W+LG GWT IGA NTMCLAYVT+LVE+RML Q+
Sbjct: 234 SGLWYYCRHPNYFGEQLWWWGLVVFAWSLGVGWTFIGAFVNTMCLAYVTRLVEDRMLKQE 293
Query: 301 NRAEAYRLYQRTTSVWVPWFKSSPLGLKSKN 331
+RAEA+R+YQ TTSVW+PWFKSSP G+K+KN
Sbjct: 294 SRAEAFRVYQNTTSVWIPWFKSSPSGVKNKN 324
>Glyma07g05300.1
Length = 325
Score = 493 bits (1268), Expect = e-139, Method: Compositional matrix adjust.
Identities = 237/332 (71%), Positives = 270/332 (81%), Gaps = 7/332 (2%)
Query: 1 MGSGTAVSNMKNAILAFLVPLPSILFYLSFLSATNGSVADPKSPSFTSTVWTWCYHYPXX 60
M SG N+KNA++AFLVPLPSI FYLSFL+ + + SP F ST+W+WCYH+P
Sbjct: 1 MASG---RNLKNAVIAFLVPLPSIFFYLSFLNHYDSA----NSPLFWSTLWSWCYHHPLL 53
Query: 61 XXXXXXXXXXXXXXWVIGLIQSSHWMIDPYWTVIPVMLVHYYSAHPLAQYHCWRSRIVIL 120
W IGLIQSSHWMIDPYWTVIPVMLVHYY+ HPLA Y WRSRIVIL
Sbjct: 54 LANALFFLNVNVLFWAIGLIQSSHWMIDPYWTVIPVMLVHYYATHPLAHYDWWRSRIVIL 113
Query: 121 LTWVWSIRLIHNYFRREKWQWGVREDWRFTDMSHQYGSHWWWVSFFSIYVPQQLFLMGLS 180
LTWVWS RL HNYFRRE+WQWG REDWRFT+MS QYG WWWVSFF++YV QQ+FL+ LS
Sbjct: 114 LTWVWSARLTHNYFRRERWQWGAREDWRFTEMSQQYGKQWWWVSFFAVYVSQQMFLIALS 173
Query: 181 LPFYVIHSVNQPLSIWDLLATIVCVSGIVIAYFADTQLHNFVSRNNKLKGQGKSVVPVLD 240
LP Y +H+ N+PLS+WDL+AT+VC+ GIVIAYFADTQL+ FVSRNNKLKG GK VV VLD
Sbjct: 174 LPLYAVHTFNEPLSMWDLVATVVCLCGIVIAYFADTQLYEFVSRNNKLKGLGKPVVFVLD 233
Query: 241 NGLWYYSRHPNYFGEQLWWWGLVVFTWNLGHGWTVIGALANTMCLAYVTKLVENRMLSQD 300
+GLWYY RHPNYFGEQLWWWGLVVF WNLGHGWT IGAL NTMCLAYVT+LVE+RML Q
Sbjct: 234 SGLWYYCRHPNYFGEQLWWWGLVVFAWNLGHGWTFIGALVNTMCLAYVTRLVEDRMLKQK 293
Query: 301 NRAEAYRLYQRTTSVWVPWFKSSPLGLKSKNV 332
+RAEA+R+YQ+ TSVWVPWFKSSP G+K+KN
Sbjct: 294 SRAEAFRVYQKKTSVWVPWFKSSPSGVKNKNA 325
>Glyma19g45120.2
Length = 240
Score = 428 bits (1100), Expect = e-120, Method: Compositional matrix adjust.
Identities = 200/247 (80%), Positives = 220/247 (89%), Gaps = 7/247 (2%)
Query: 86 MIDPYWTVIPVMLVHYYSAHPLAQYHCWRSRIVILLTWVWSIRLIHNYFRREKWQWGVRE 145
MIDPYWTVIPVMLVHYYS HPLAQY WRSRIV LLTW WS+RLIHNYFRREKWQWG RE
Sbjct: 1 MIDPYWTVIPVMLVHYYSTHPLAQYQWWRSRIVTLLTWAWSVRLIHNYFRREKWQWGARE 60
Query: 146 DWRFTDMSHQYGSHWWWVSFFSIYVPQQLFLMGLSLPFYVIHSVNQPLSIWDLLATIVCV 205
DWRFTD+SH+YG HWWW SFF+IYVPQQ+FL+GLSLPFYVIHSVNQPLS+WDL+A +VCV
Sbjct: 61 DWRFTDLSHRYGRHWWWASFFAIYVPQQVFLIGLSLPFYVIHSVNQPLSMWDLVAIVVCV 120
Query: 206 SGIVIAYFADTQLHNFVSRNNKLKGQGKSVVPVLDNGLWYYSRHPNYFGEQLWWWGLVVF 265
SGIV AY ADTQL+NFVSR NK VP+LD GLWYYSRHPNYFGEQ+WWWG+ VF
Sbjct: 121 SGIVTAYIADTQLYNFVSRKNK-------EVPILDKGLWYYSRHPNYFGEQVWWWGMAVF 173
Query: 266 TWNLGHGWTVIGALANTMCLAYVTKLVENRMLSQDNRAEAYRLYQRTTSVWVPWFKSSPL 325
WNLGHGW IGALANTMCLAYVTKLVE+RML QD+RAEA+RLYQ+TTS+W+PWFKSSPL
Sbjct: 174 AWNLGHGWAFIGALANTMCLAYVTKLVEDRMLKQDSRAEAFRLYQKTTSLWIPWFKSSPL 233
Query: 326 GLKSKNV 332
GLKSK+
Sbjct: 234 GLKSKDA 240
>Glyma05g33940.3
Length = 291
Score = 81.3 bits (199), Expect = 1e-15, Method: Compositional matrix adjust.
Identities = 68/214 (31%), Positives = 114/214 (53%), Gaps = 25/214 (11%)
Query: 113 WRSRIVILLTWVWSIRL-IHNYFRREKWQWGVREDWRFTDMSHQYGSHWWWVSFFSIYVP 171
+R I+ L +W IRL + FR QWG ED RF +M G ++ F I+
Sbjct: 61 FRQIILTLFVGIWGIRLGLFLLFR--ILQWG--EDRRFDEMRSNLGR----LAIFWIF-- 110
Query: 172 QQLFLMGLSLPFYVIHSVNQP--LSIWDLLATIVCVSGIVIAYFADTQLHNFVSRNNKLK 229
Q +++ +SLP V+++ ++ L + D++ I+ G ++ AD Q +F R+++ +
Sbjct: 111 QAVWVWAVSLPVTVVNASDRNPFLQVVDIVGWILWAVGFIVEGTADQQKLHF-KRSSENR 169
Query: 230 GQGKSVVPVLDNGLWYYSRHPNYFGEQLWWWGLVVFTWNLGHG---WTVIGALANTMCLA 286
G+ +V GLW YSRHPNYFGE L WWG+ V + + G +IG + T+ L
Sbjct: 170 GKWCNV------GLWKYSRHPNYFGEILLWWGIFVASTPVLKGAEWLVIIGPIFLTLLLL 223
Query: 287 YVTK--LVENRMLSQDNRAEAYRLYQRTTSVWVP 318
+V+ L+E+ + + YR+Y++ TS +P
Sbjct: 224 FVSGIPLLEDSADKKFGNVDGYRIYKKRTSPLIP 257
>Glyma05g33940.1
Length = 306
Score = 80.9 bits (198), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 68/214 (31%), Positives = 114/214 (53%), Gaps = 25/214 (11%)
Query: 113 WRSRIVILLTWVWSIRL-IHNYFRREKWQWGVREDWRFTDMSHQYGSHWWWVSFFSIYVP 171
+R I+ L +W IRL + FR QWG ED RF +M G ++ F I+
Sbjct: 61 FRQIILTLFVGIWGIRLGLFLLFR--ILQWG--EDRRFDEMRSNLGR----LAIFWIF-- 110
Query: 172 QQLFLMGLSLPFYVIHSVNQP--LSIWDLLATIVCVSGIVIAYFADTQLHNFVSRNNKLK 229
Q +++ +SLP V+++ ++ L + D++ I+ G ++ AD Q +F R+++ +
Sbjct: 111 QAVWVWAVSLPVTVVNASDRNPFLQVVDIVGWILWAVGFIVEGTADQQKLHF-KRSSENR 169
Query: 230 GQGKSVVPVLDNGLWYYSRHPNYFGEQLWWWGLVVFTWNLGHG---WTVIGALANTMCLA 286
G+ +V GLW YSRHPNYFGE L WWG+ V + + G +IG + T+ L
Sbjct: 170 GKWCNV------GLWKYSRHPNYFGEILLWWGIFVASTPVLKGAEWLVIIGPIFLTLLLL 223
Query: 287 YVTK--LVENRMLSQDNRAEAYRLYQRTTSVWVP 318
+V+ L+E+ + + YR+Y++ TS +P
Sbjct: 224 FVSGIPLLEDSADKKFGNVDGYRIYKKRTSPLIP 257
>Glyma05g33940.2
Length = 286
Score = 77.0 bits (188), Expect = 2e-14, Method: Compositional matrix adjust.
Identities = 66/211 (31%), Positives = 112/211 (53%), Gaps = 25/211 (11%)
Query: 113 WRSRIVILLTWVWSIRL-IHNYFRREKWQWGVREDWRFTDMSHQYGSHWWWVSFFSIYVP 171
+R I+ L +W IRL + FR QWG ED RF +M G ++ F I+
Sbjct: 61 FRQIILTLFVGIWGIRLGLFLLFR--ILQWG--EDRRFDEMRSNLGR----LAIFWIF-- 110
Query: 172 QQLFLMGLSLPFYVIHSVNQP--LSIWDLLATIVCVSGIVIAYFADTQLHNFVSRNNKLK 229
Q +++ +SLP V+++ ++ L + D++ I+ G ++ AD Q +F R+++ +
Sbjct: 111 QAVWVWAVSLPVTVVNASDRNPFLQVVDIVGWILWAVGFIVEGTADQQKLHF-KRSSENR 169
Query: 230 GQGKSVVPVLDNGLWYYSRHPNYFGEQLWWWGLVVFTWNLGHG---WTVIGALANTMCLA 286
G+ +V GLW YSRHPNYFGE L WWG+ V + + G +IG + T+ L
Sbjct: 170 GKWCNV------GLWKYSRHPNYFGEILLWWGIFVASTPVLKGAEWLVIIGPIFLTLLLL 223
Query: 287 YVTK--LVENRMLSQDNRAEAYRLYQRTTSV 315
+V+ L+E+ + + YR+Y++ T +
Sbjct: 224 FVSGIPLLEDSADKKFGNVDGYRIYKKRTRL 254
>Glyma08g05750.1
Length = 291
Score = 74.3 bits (181), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 59/190 (31%), Positives = 103/190 (54%), Gaps = 22/190 (11%)
Query: 140 QWGVREDWRFTDMSHQYGSHWWWVSFFSIYVPQQLFLMGLSLPFYVIHSVNQP--LSIWD 197
QWG ED RF +M G ++ F I+ Q +++ +SLP ++++ ++ L + D
Sbjct: 87 QWG--EDRRFDEMRSNLGR----LAIFWIF--QAVWVWVVSLPVTLVNASDRNPFLQVVD 138
Query: 198 LLATIVCVSGIVIAYFADTQLHNFVSRNNKLKGQGKSVVPVLDNGLWYYSRHPNYFGEQL 257
++ I+ G ++ AD Q +F R+++ +G+ +V GLW YSRHPNYFGE L
Sbjct: 139 IVGWILWAVGFIVEGTADQQKLHF-KRSSENRGKWCNV------GLWKYSRHPNYFGEIL 191
Query: 258 WWWGLVVFTWNLGHG---WTVIGALANTMCLAYVTK--LVENRMLSQDNRAEAYRLYQRT 312
WWG+ V + + G +IG + T+ L +V+ L+E+ + + YR+Y++
Sbjct: 192 LWWGIFVASTPVLKGAEWLVIIGPIFLTLLLLFVSGIPLLEDSADKKFGNVDGYRIYKKR 251
Query: 313 TSVWVPWFKS 322
TS +P +S
Sbjct: 252 TSPLIPLPRS 261