Miyakogusa Predicted Gene

Lj1g3v3330210.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v3330210.1 tr|G7J985|G7J985_MEDTR Cytoplasmic tRNA
2-thiolation protein OS=Medicago truncatula GN=MTR_3g118250
,87.93,0,ATP_bind_3,tRNA(Ile)-lysidine/2-thiocytidine synthase; no
description,Rossmann-like alpha/beta/alpha,CUFF.30464.1
         (349 letters)

Database: Glyma1.pep 
           75,778 sequences; 25,431,882 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Glyma04g00350.1                                                       605   e-173
Glyma04g00350.2                                                       478   e-135
Glyma04g00350.3                                                       408   e-114

>Glyma04g00350.1 
          Length = 353

 Score =  605 bits (1560), Expect = e-173,   Method: Compositional matrix adjust.
 Identities = 296/353 (83%), Positives = 315/353 (89%), Gaps = 4/353 (1%)

Query: 1   MEGEG-ERKRRS-LCCVCNERRPSLKRPKTLEHICRECFYTVFEDEIHQVIVTNNLFRRG 58
           MEGEG ERKR + LCCVC + R S+KRPKTLE ICRECFY  FE EIHQVI+ N LF  G
Sbjct: 1   MEGEGGERKRGARLCCVCKKSRASVKRPKTLEQICRECFYDAFESEIHQVILENQLFSPG 60

Query: 59  ERVAIGASGGKDSTVLAYVLSKLNRVHDYGLHLFLLSVDEGITGYRDDSLQTVHRNQIQY 118
           ERVAIGASGGKDSTVLAYVLSKLNR+H+YGLHLFLLSVDEGITGYRDDSL+TVHRNQIQY
Sbjct: 61  ERVAIGASGGKDSTVLAYVLSKLNRLHNYGLHLFLLSVDEGITGYRDDSLETVHRNQIQY 120

Query: 119 GLPLQVVSYMDLYGWTMDDIVKLIGLKNNCTFCGVFRRQALDRGAALLKADKLVTGHNAD 178
           GLPL+VVSY DLYGWTMD+IV++IGLKNNCTFCGVFRRQALDRGA+LLK DK+VTGHNAD
Sbjct: 121 GLPLKVVSYKDLYGWTMDEIVRVIGLKNNCTFCGVFRRQALDRGASLLKVDKVVTGHNAD 180

Query: 179 DIAETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKRLDYFST 238
           DIAETVLLN+LRGD+ARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFK+LDYFST
Sbjct: 181 DIAETVLLNLLRGDVARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFST 240

Query: 239 ECIYSPNAYRGFAREFIKDLERMRPRAILDIIKSGENFRISTTTKMPEQGTCERCGYISS 298
           ECIYSPNAYRGFAREFIKDLER+RPRAILDIIKSGENFRIST+TKMPEQGTCERCGYISS
Sbjct: 241 ECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGENFRISTSTKMPEQGTCERCGYISS 300

Query: 299 QKWCKACVLLDGLNRGLPKLXX--XXXXXXXXXXXXXXXXKSIESKQCGTLDF 349
           QKWCKACVLLDGLNRGLPKL                    KSI+ KQC TLDF
Sbjct: 301 QKWCKACVLLDGLNRGLPKLGIGRSRGSVASDNGNESKVEKSIQGKQCTTLDF 353


>Glyma04g00350.2 
          Length = 265

 Score =  478 bits (1231), Expect = e-135,   Method: Compositional matrix adjust.
 Identities = 231/264 (87%), Positives = 248/264 (93%), Gaps = 2/264 (0%)

Query: 1   MEGEG-ERKRRS-LCCVCNERRPSLKRPKTLEHICRECFYTVFEDEIHQVIVTNNLFRRG 58
           MEGEG ERKR + LCCVC + R S+KRPKTLE ICRECFY  FE EIHQVI+ N LF  G
Sbjct: 1   MEGEGGERKRGARLCCVCKKSRASVKRPKTLEQICRECFYDAFESEIHQVILENQLFSPG 60

Query: 59  ERVAIGASGGKDSTVLAYVLSKLNRVHDYGLHLFLLSVDEGITGYRDDSLQTVHRNQIQY 118
           ERVAIGASGGKDSTVLAYVLSKLNR+H+YGLHLFLLSVDEGITGYRDDSL+TVHRNQIQY
Sbjct: 61  ERVAIGASGGKDSTVLAYVLSKLNRLHNYGLHLFLLSVDEGITGYRDDSLETVHRNQIQY 120

Query: 119 GLPLQVVSYMDLYGWTMDDIVKLIGLKNNCTFCGVFRRQALDRGAALLKADKLVTGHNAD 178
           GLPL+VVSY DLYGWTMD+IV++IGLKNNCTFCGVFRRQALDRGA+LLK DK+VTGHNAD
Sbjct: 121 GLPLKVVSYKDLYGWTMDEIVRVIGLKNNCTFCGVFRRQALDRGASLLKVDKVVTGHNAD 180

Query: 179 DIAETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKRLDYFST 238
           DIAETVLLN+LRGD+ARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFK+LDYFST
Sbjct: 181 DIAETVLLNLLRGDVARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFST 240

Query: 239 ECIYSPNAYRGFAREFIKDLERMR 262
           ECIYSPNAYRGFAREFIKDLER+R
Sbjct: 241 ECIYSPNAYRGFAREFIKDLERIR 264


>Glyma04g00350.3 
          Length = 232

 Score =  408 bits (1048), Expect = e-114,   Method: Compositional matrix adjust.
 Identities = 197/229 (86%), Positives = 213/229 (93%), Gaps = 2/229 (0%)

Query: 1   MEGEG-ERKRRS-LCCVCNERRPSLKRPKTLEHICRECFYTVFEDEIHQVIVTNNLFRRG 58
           MEGEG ERKR + LCCVC + R S+KRPKTLE ICRECFY  FE EIHQVI+ N LF  G
Sbjct: 1   MEGEGGERKRGARLCCVCKKSRASVKRPKTLEQICRECFYDAFESEIHQVILENQLFSPG 60

Query: 59  ERVAIGASGGKDSTVLAYVLSKLNRVHDYGLHLFLLSVDEGITGYRDDSLQTVHRNQIQY 118
           ERVAIGASGGKDSTVLAYVLSKLNR+H+YGLHLFLLSVDEGITGYRDDSL+TVHRNQIQY
Sbjct: 61  ERVAIGASGGKDSTVLAYVLSKLNRLHNYGLHLFLLSVDEGITGYRDDSLETVHRNQIQY 120

Query: 119 GLPLQVVSYMDLYGWTMDDIVKLIGLKNNCTFCGVFRRQALDRGAALLKADKLVTGHNAD 178
           GLPL+VVSY DLYGWTMD+IV++IGLKNNCTFCGVFRRQALDRGA+LLK DK+VTGHNAD
Sbjct: 121 GLPLKVVSYKDLYGWTMDEIVRVIGLKNNCTFCGVFRRQALDRGASLLKVDKVVTGHNAD 180

Query: 179 DIAETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMY 227
           DIAETVLLN+LRGD+ARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVM+
Sbjct: 181 DIAETVLLNLLRGDVARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMH 229