Miyakogusa Predicted Gene
- Lj1g3v2418700.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj1g3v2418700.1 tr|G7KW01|G7KW01_MEDTR D-alanine--D-alanine
ligase OS=Medicago truncatula GN=MTR_7g071990 PE=4
SV=1,82.79,0,SUBFAMILY NOT NAMED,NULL; D-ALANINE--D-ALANINE
LIGASE,D-alanine--D-alanine ligase; PreATP-grasp doma,CUFF.29126.1
(942 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma09g41600.1 1543 0.0
Glyma18g44100.1 1504 0.0
Glyma16g22350.1 98 4e-20
Glyma16g22330.1 91 9e-18
>Glyma09g41600.1
Length = 936
Score = 1543 bits (3995), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 760/923 (82%), Positives = 817/923 (88%), Gaps = 37/923 (4%)
Query: 40 LRRFGLKHELRTCRATPSIARA---SAREVVVVDGGVEKTKEEGRVLKVG---------- 86
++ F KHE TC A SA EV VV+ G EK RVLK+G
Sbjct: 31 VKLFRSKHEASTCHGARVAAAVPRASAPEVAVVEKGREKV----RVLKLGLICGGPSAER 86
Query: 87 -----SARSVLDHLQGDDIHVSCYYIDINLNAFAISSAQVYSNTPADFDFKLESLAQSFS 141
SARSVLDHLQGDD+HVSCYYID NLNAFAISSAQVYSNTPADFDFKLESLAQSF
Sbjct: 87 GISLNSARSVLDHLQGDDLHVSCYYIDCNLNAFAISSAQVYSNTPADFDFKLESLAQSFQ 146
Query: 142 TLDDMAEHLATSVDIVFPVIHGQFGEDGRIQELLEKYNVPYVGTGSKECCQAFDKYKASL 201
TL D+A+HLAT+VDIVFPVIHGQFGEDG IQELLEKYNVP+VGTGSKECCQAFDK+KASL
Sbjct: 147 TLSDLAKHLATAVDIVFPVIHGQFGEDGGIQELLEKYNVPFVGTGSKECCQAFDKHKASL 206
Query: 202 ELRQHGFVTVPSFLVQGHKTDKSELSEWFRKHQLDPDLGKVVVKPTRGGSSIGVGVAYGV 261
ELR+HGF+TVPSFLVQG++TDKSELSEWF+KHQLDPDLGKVVVKPTRGGSSIGVGVAYGV
Sbjct: 207 ELRKHGFITVPSFLVQGYETDKSELSEWFKKHQLDPDLGKVVVKPTRGGSSIGVGVAYGV 266
Query: 262 NDSLVKANEIMTEGIDNKVLIEIFLEGGSEFTAIVLDVGSGSDCCPVVLLPTEVELQFLG 321
NDSLVKANEIM+EGID KVLIEIFLEGG+EFTAIVLDVGS D PVVLLPTE
Sbjct: 267 NDSLVKANEIMSEGIDKKVLIEIFLEGGNEFTAIVLDVGSDLDSFPVVLLPTE------- 319
Query: 322 ANDVKENDAIFNYRRKYLPTQQVAYHTPPRFPLDVIDNIRKGASLIFQRLCLQDFARIDG 381
NDAIFNYRRKYLPTQQVAYHTPPRFPLDVI+NIRKGASL+FQRLCLQDFARIDG
Sbjct: 320 ------NDAIFNYRRKYLPTQQVAYHTPPRFPLDVIENIRKGASLLFQRLCLQDFARIDG 373
Query: 382 WFLPNSDSKLS-CSENEFGRTEAGTILFTDINLISGMEQTSFLFQQASKVGFSHKNILRS 440
WFLPNS SKLS S++EFGRTE+G I+FTDIN+ISGMEQTSFLFQQASKVGFSH NILRS
Sbjct: 374 WFLPNSSSKLSPFSQSEFGRTESGAIIFTDINMISGMEQTSFLFQQASKVGFSHTNILRS 433
Query: 441 VVRHACLRFPNLPSESGISAQVPTRSKSFELNKAFPFPEGARKVFVIFGGDTSERQVSLM 500
++ HACLRFPNL S SGIS Q+P+RSKS + NK+F EG RKVFVIFGG+TSERQVSLM
Sbjct: 434 IIHHACLRFPNLASVSGISGQLPSRSKSLQRNKSFSRREGTRKVFVIFGGNTSERQVSLM 493
Query: 501 SGTNVWLNLLAFHDLEVTPFLLASTSECESNVDMVKKADDVRDRTIWSLPYSLVLRHTTE 560
SGTNVWLNLLAFHDLEVTP LL+ TSEC S++DM KKADDV +RT+WSLPYSLVLRHTTE
Sbjct: 494 SGTNVWLNLLAFHDLEVTPCLLSPTSECASSIDMGKKADDVMNRTVWSLPYSLVLRHTTE 553
Query: 561 EVLAACKEAIEPDRAALTSNLRKKVVNELMEGLKDHNWFTGFDIADELPMKFSLRLWIKS 620
EVL AC EAIEP+ AA+TS LRKKV+N+LMEGLKDHNWFTGFDIAD+LP+KFSLR WIK
Sbjct: 554 EVLDACMEAIEPEHAAITSELRKKVMNDLMEGLKDHNWFTGFDIADDLPVKFSLRQWIKL 613
Query: 621 AKEVQATVFIAVHGGIGEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVALKHLANF 680
AKEVQATVFIAVHGGIGEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVALKHLAN
Sbjct: 614 AKEVQATVFIAVHGGIGEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVALKHLANS 673
Query: 681 GVLTINKDVRRKDDLFDKPITDIWHDLTQKLQCETLCVKPARDGCSTGVARLCCSDDLAI 740
GVLTINKDVR+KDDL +KPI D WHDLT+KLQC+TLCVKPA+DGCSTGVARLCC +DLAI
Sbjct: 674 GVLTINKDVRQKDDLSNKPINDTWHDLTRKLQCQTLCVKPAKDGCSTGVARLCCPEDLAI 733
Query: 741 YVKALEDCLLRIPPNSFSKAHSIIEMPNPPPELLIFEPFIETDEIVVTSKFKE-TGHDLM 799
YVKALEDCLLRIPPN SKAH +IEMPNPPPE LIFEPFIETDEI+VTSKF++ TGH L
Sbjct: 734 YVKALEDCLLRIPPNCLSKAHGMIEMPNPPPEHLIFEPFIETDEIIVTSKFEDATGHGLT 793
Query: 800 WKGHSRWVEITVGVIGKRGSMHSLSPSVTVKETGDILSLEEKFQGGTGINLTPPPLSIMS 859
WKGHSRWVEITVGVIGKRGSMHSLSPSVTVKE+GDILSLEEKFQGGTGINLTPPPLSIMS
Sbjct: 794 WKGHSRWVEITVGVIGKRGSMHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIMS 853
Query: 860 EKALQRCKKHIEQIANTLQLEGFSRIDAFVNVDNGEVLIIEINTVPGMTPSTVLIHQALA 919
E AL+RCK+HIE IANTLQLEGFSRIDAFVNVD+GEVLIIE+NTVPGMTPSTVLIHQAL
Sbjct: 854 ENALKRCKQHIELIANTLQLEGFSRIDAFVNVDSGEVLIIEVNTVPGMTPSTVLIHQALV 913
Query: 920 EQPPLYPHQFFRTLLDLGSVRSM 942
EQPPLYPHQFFR LLDL S RSM
Sbjct: 914 EQPPLYPHQFFRKLLDLASERSM 936
>Glyma18g44100.1
Length = 912
Score = 1504 bits (3894), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 748/907 (82%), Positives = 805/907 (88%), Gaps = 32/907 (3%)
Query: 53 RATPSIARASAREVVVVDGGVEKTKEEGRVLKVG---------------SARSVLDHLQG 97
R + ARA VV G VEK +E+GRVLKVG SARS G
Sbjct: 21 RRSTRPARAMLHVAVVGGGVVEKGREKGRVLKVGLICGGPSAERGISLNSARS------G 74
Query: 98 DDIHVSCYYIDINLNAFAISSAQVYSNTPADFDFKLESLAQSFSTLDDMAEHLATSVDIV 157
DD+HVSCYYID NLNAFAISSAQVYSNTPADFDFKLESLAQSF TL D+A+HLAT+VDIV
Sbjct: 75 DDLHVSCYYIDCNLNAFAISSAQVYSNTPADFDFKLESLAQSFQTLSDLAKHLATAVDIV 134
Query: 158 FPVIHGQFGEDGRIQELLEKYNVPYVGTGSKECCQAFDKYKASLELRQHGFVTVPSFLVQ 217
FPVIHGQFGEDG IQELLEKYNVP+VGTGSKECCQAFDK+KASLELR+HGF+TVPSFLVQ
Sbjct: 135 FPVIHGQFGEDGGIQELLEKYNVPFVGTGSKECCQAFDKHKASLELRKHGFITVPSFLVQ 194
Query: 218 GHKTDKSELSEWFRKHQLDPDLGKVVVKPTRGGSSIGVGVAYGVNDSLVKANEIMTEGID 277
G++T KSELSEWF KHQLDPDLGKVVVKPTRGGSSIGV VAYGVNDSLVKANEIM+EGID
Sbjct: 195 GYETKKSELSEWFEKHQLDPDLGKVVVKPTRGGSSIGVRVAYGVNDSLVKANEIMSEGID 254
Query: 278 NKVLIEIFLEGGSEFTAIVLDVGSGSDCCPVVLLPTEVELQFLGANDVKENDAIFNYRRK 337
NKVLIEI+LEGGSEFTAIVLDVGS SD PVVLLPTEVELQF GANDVKENDAIFNYRRK
Sbjct: 255 NKVLIEIYLEGGSEFTAIVLDVGSASDSFPVVLLPTEVELQFRGANDVKENDAIFNYRRK 314
Query: 338 YLPTQQVAYHTPPRFPLDVIDNIRKGASLIFQRLCLQDFARIDGWFLPNSDSKLSCS-EN 396
YLPTQQVAYHTPPRFPLDVI+NIRKGASLIFQ+LCLQDFARIDGWFLPNS SKLS S E+
Sbjct: 315 YLPTQQVAYHTPPRFPLDVIENIRKGASLIFQQLCLQDFARIDGWFLPNSSSKLSPSPES 374
Query: 397 EFGRTEAGTILFTDINLISGMEQTSFLFQQASKVGFSHKNILRSVVRHACLRFPNLPSES 456
EFGRTE+GTI+FTDINLISGMEQTSFLFQQASKVGFSH NILRS++ HACLRFPNL S S
Sbjct: 375 EFGRTESGTIIFTDINLISGMEQTSFLFQQASKVGFSHTNILRSIIHHACLRFPNLASVS 434
Query: 457 GISAQVPTRSKSFELNKAFPFPEGARKVFVIFGGDTSERQVSLMSGTNVWLNLLAFHDLE 516
GIS Q+P+RSKS + +K+F EG RKVFVIFGG+TSERQVSLMSGTNVWLNLLAFHDLE
Sbjct: 435 GISGQLPSRSKSLQQSKSFSRHEGTRKVFVIFGGNTSERQVSLMSGTNVWLNLLAFHDLE 494
Query: 517 VTPFLLASTSECESNVDMVKKADDVRDRTIWSLPYSLVLRHTTEEVLAACKEAIEPDRAA 576
VTP LL+ TS + + D YSLVLRHTTEEVL AC EAIEP+RAA
Sbjct: 495 VTPCLLSPTS---FQLLAFAFRHSIHDH------YSLVLRHTTEEVLDACMEAIEPERAA 545
Query: 577 LTSNLRKKVVNELMEGLKDHNWFTGFDIADELPMKFSLRLWIKSAKEVQATVFIAVHGGI 636
+TS+LRKKV+N+LMEGLKDHNWFTGFDIAD+LP KFSLR WIK AKEVQAT+FIAVHGGI
Sbjct: 546 ITSDLRKKVMNDLMEGLKDHNWFTGFDIADDLPAKFSLRQWIKLAKEVQATIFIAVHGGI 605
Query: 637 GEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVALKHLANFGVLTINKDVRRKDDLF 696
GEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVA+KHLAN GVLTINK+VR+KDDL
Sbjct: 606 GEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVAIKHLANSGVLTINKNVRQKDDLS 665
Query: 697 DKPITDIWHDLTQKLQCETLCVKPARDGCSTGVARLCCSDDLAIYVKALEDCLLRIPPNS 756
+KPI+D WHDLT+KLQC+TLCVKPA+DGCSTGVARLCCS+DLAIYV+ALEDCLLRIPPNS
Sbjct: 666 NKPISDTWHDLTRKLQCQTLCVKPAKDGCSTGVARLCCSEDLAIYVRALEDCLLRIPPNS 725
Query: 757 FSKAHSIIEMPNPPPELLIFEPFIETDEIVVTSKFKE-TGHDLMWKGHSRWVEITVGVIG 815
SKAH +IEMPNPPPE LIFEPFIETDEI+VTSKF++ TGH L WKGHSRWVEITVGVIG
Sbjct: 726 LSKAHGMIEMPNPPPEYLIFEPFIETDEIIVTSKFEDATGHGLTWKGHSRWVEITVGVIG 785
Query: 816 KRGSMHSLSPSVTVKETGDILSLEEKFQGGTGINLTPPPLSIMSEKALQRCKKHIEQIAN 875
KRGSMHSLSPSVTVKE+GDILSLEEKFQGGTGINLTPPPLSIMSE AL+RCK+HIE IAN
Sbjct: 786 KRGSMHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIMSENALKRCKQHIELIAN 845
Query: 876 TLQLEGFSRIDAFVNVDNGEVLIIEINTVPGMTPSTVLIHQALAEQPPLYPHQFFRTLLD 935
TLQLEGFSRIDAFVNVD+GEVLIIE+NTVPGMTPSTVLIHQAL EQPPLYPHQFFR LLD
Sbjct: 846 TLQLEGFSRIDAFVNVDSGEVLIIEVNTVPGMTPSTVLIHQALVEQPPLYPHQFFRKLLD 905
Query: 936 LGSVRSM 942
L S RSM
Sbjct: 906 LASERSM 912
>Glyma16g22350.1
Length = 310
Score = 98.2 bits (243), Expect = 4e-20, Method: Compositional matrix adjust.
Identities = 87/231 (37%), Positives = 103/231 (44%), Gaps = 69/231 (29%)
Query: 289 GSEFTAIVLDVGSGSDCCPVVLLPTEVELQFLGANDVKENDAIFNYRRKYLPTQQVAYHT 348
GSE IVLDVG SD VVLL TE Q YL T + +
Sbjct: 1 GSELATIVLDVGFDSDSFLVVLLLTEKMTQ-------------------YLITTRSTFQ- 40
Query: 349 PPRFPLDVIDNIRKGASLIFQRLCLQDFARIDGWFLPNSDSKLSCSEN-EFGRTEAGTIL 407
S++FQ LC QDF RIDGWFLPNS SKL S EFGR E+G I+
Sbjct: 41 ----------------SMLFQWLCTQDFERIDGWFLPNSSSKLLSSSKSEFGRIESGVII 84
Query: 408 FTDINLISGMEQTSFLFQQASKVG-FSHKNILRSVVRHACLRFPNLPSESGISAQVPTRS 466
FTD NLIS MEQ FLFQQASK +S N++ +V + + F + I++
Sbjct: 85 FTDTNLISSMEQIGFLFQQASKFRIWSPSNLIHELVVYQ-VSFQAGQNPCHITSHFLAMK 143
Query: 467 KSFELNKAFPFPEGARKVFVIFGGDTSERQVSLMSGTNVWLNLLAFHDLEV 517
EL K F E LNLLAFHD+ V
Sbjct: 144 ---ELEKCLSFLE---------------------------LNLLAFHDVSV 164
>Glyma16g22330.1
Length = 55
Score = 90.5 bits (223), Expect = 9e-18, Method: Compositional matrix adjust.
Identities = 41/55 (74%), Positives = 47/55 (85%)
Query: 142 TLDDMAEHLATSVDIVFPVIHGQFGEDGRIQELLEKYNVPYVGTGSKECCQAFDK 196
TL D+A+HLAT+V+IVF + HGQFGED IQEL E YNVP+VGTGSKE CQAFDK
Sbjct: 1 TLSDLAKHLATTVEIVFLIRHGQFGEDADIQELSETYNVPFVGTGSKESCQAFDK 55