Miyakogusa Predicted Gene

Lj1g3v2418700.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v2418700.1 tr|G7KW01|G7KW01_MEDTR D-alanine--D-alanine
ligase OS=Medicago truncatula GN=MTR_7g071990 PE=4
SV=1,82.79,0,SUBFAMILY NOT NAMED,NULL; D-ALANINE--D-ALANINE
LIGASE,D-alanine--D-alanine ligase; PreATP-grasp doma,CUFF.29126.1
         (942 letters)

Database: Glyma1.pep 
           75,778 sequences; 25,431,882 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Glyma09g41600.1                                                      1543   0.0  
Glyma18g44100.1                                                      1504   0.0  
Glyma16g22350.1                                                        98   4e-20
Glyma16g22330.1                                                        91   9e-18

>Glyma09g41600.1 
          Length = 936

 Score = 1543 bits (3995), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 760/923 (82%), Positives = 817/923 (88%), Gaps = 37/923 (4%)

Query: 40  LRRFGLKHELRTCRATPSIARA---SAREVVVVDGGVEKTKEEGRVLKVG---------- 86
           ++ F  KHE  TC      A     SA EV VV+ G EK     RVLK+G          
Sbjct: 31  VKLFRSKHEASTCHGARVAAAVPRASAPEVAVVEKGREKV----RVLKLGLICGGPSAER 86

Query: 87  -----SARSVLDHLQGDDIHVSCYYIDINLNAFAISSAQVYSNTPADFDFKLESLAQSFS 141
                SARSVLDHLQGDD+HVSCYYID NLNAFAISSAQVYSNTPADFDFKLESLAQSF 
Sbjct: 87  GISLNSARSVLDHLQGDDLHVSCYYIDCNLNAFAISSAQVYSNTPADFDFKLESLAQSFQ 146

Query: 142 TLDDMAEHLATSVDIVFPVIHGQFGEDGRIQELLEKYNVPYVGTGSKECCQAFDKYKASL 201
           TL D+A+HLAT+VDIVFPVIHGQFGEDG IQELLEKYNVP+VGTGSKECCQAFDK+KASL
Sbjct: 147 TLSDLAKHLATAVDIVFPVIHGQFGEDGGIQELLEKYNVPFVGTGSKECCQAFDKHKASL 206

Query: 202 ELRQHGFVTVPSFLVQGHKTDKSELSEWFRKHQLDPDLGKVVVKPTRGGSSIGVGVAYGV 261
           ELR+HGF+TVPSFLVQG++TDKSELSEWF+KHQLDPDLGKVVVKPTRGGSSIGVGVAYGV
Sbjct: 207 ELRKHGFITVPSFLVQGYETDKSELSEWFKKHQLDPDLGKVVVKPTRGGSSIGVGVAYGV 266

Query: 262 NDSLVKANEIMTEGIDNKVLIEIFLEGGSEFTAIVLDVGSGSDCCPVVLLPTEVELQFLG 321
           NDSLVKANEIM+EGID KVLIEIFLEGG+EFTAIVLDVGS  D  PVVLLPTE       
Sbjct: 267 NDSLVKANEIMSEGIDKKVLIEIFLEGGNEFTAIVLDVGSDLDSFPVVLLPTE------- 319

Query: 322 ANDVKENDAIFNYRRKYLPTQQVAYHTPPRFPLDVIDNIRKGASLIFQRLCLQDFARIDG 381
                 NDAIFNYRRKYLPTQQVAYHTPPRFPLDVI+NIRKGASL+FQRLCLQDFARIDG
Sbjct: 320 ------NDAIFNYRRKYLPTQQVAYHTPPRFPLDVIENIRKGASLLFQRLCLQDFARIDG 373

Query: 382 WFLPNSDSKLS-CSENEFGRTEAGTILFTDINLISGMEQTSFLFQQASKVGFSHKNILRS 440
           WFLPNS SKLS  S++EFGRTE+G I+FTDIN+ISGMEQTSFLFQQASKVGFSH NILRS
Sbjct: 374 WFLPNSSSKLSPFSQSEFGRTESGAIIFTDINMISGMEQTSFLFQQASKVGFSHTNILRS 433

Query: 441 VVRHACLRFPNLPSESGISAQVPTRSKSFELNKAFPFPEGARKVFVIFGGDTSERQVSLM 500
           ++ HACLRFPNL S SGIS Q+P+RSKS + NK+F   EG RKVFVIFGG+TSERQVSLM
Sbjct: 434 IIHHACLRFPNLASVSGISGQLPSRSKSLQRNKSFSRREGTRKVFVIFGGNTSERQVSLM 493

Query: 501 SGTNVWLNLLAFHDLEVTPFLLASTSECESNVDMVKKADDVRDRTIWSLPYSLVLRHTTE 560
           SGTNVWLNLLAFHDLEVTP LL+ TSEC S++DM KKADDV +RT+WSLPYSLVLRHTTE
Sbjct: 494 SGTNVWLNLLAFHDLEVTPCLLSPTSECASSIDMGKKADDVMNRTVWSLPYSLVLRHTTE 553

Query: 561 EVLAACKEAIEPDRAALTSNLRKKVVNELMEGLKDHNWFTGFDIADELPMKFSLRLWIKS 620
           EVL AC EAIEP+ AA+TS LRKKV+N+LMEGLKDHNWFTGFDIAD+LP+KFSLR WIK 
Sbjct: 554 EVLDACMEAIEPEHAAITSELRKKVMNDLMEGLKDHNWFTGFDIADDLPVKFSLRQWIKL 613

Query: 621 AKEVQATVFIAVHGGIGEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVALKHLANF 680
           AKEVQATVFIAVHGGIGEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVALKHLAN 
Sbjct: 614 AKEVQATVFIAVHGGIGEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVALKHLANS 673

Query: 681 GVLTINKDVRRKDDLFDKPITDIWHDLTQKLQCETLCVKPARDGCSTGVARLCCSDDLAI 740
           GVLTINKDVR+KDDL +KPI D WHDLT+KLQC+TLCVKPA+DGCSTGVARLCC +DLAI
Sbjct: 674 GVLTINKDVRQKDDLSNKPINDTWHDLTRKLQCQTLCVKPAKDGCSTGVARLCCPEDLAI 733

Query: 741 YVKALEDCLLRIPPNSFSKAHSIIEMPNPPPELLIFEPFIETDEIVVTSKFKE-TGHDLM 799
           YVKALEDCLLRIPPN  SKAH +IEMPNPPPE LIFEPFIETDEI+VTSKF++ TGH L 
Sbjct: 734 YVKALEDCLLRIPPNCLSKAHGMIEMPNPPPEHLIFEPFIETDEIIVTSKFEDATGHGLT 793

Query: 800 WKGHSRWVEITVGVIGKRGSMHSLSPSVTVKETGDILSLEEKFQGGTGINLTPPPLSIMS 859
           WKGHSRWVEITVGVIGKRGSMHSLSPSVTVKE+GDILSLEEKFQGGTGINLTPPPLSIMS
Sbjct: 794 WKGHSRWVEITVGVIGKRGSMHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIMS 853

Query: 860 EKALQRCKKHIEQIANTLQLEGFSRIDAFVNVDNGEVLIIEINTVPGMTPSTVLIHQALA 919
           E AL+RCK+HIE IANTLQLEGFSRIDAFVNVD+GEVLIIE+NTVPGMTPSTVLIHQAL 
Sbjct: 854 ENALKRCKQHIELIANTLQLEGFSRIDAFVNVDSGEVLIIEVNTVPGMTPSTVLIHQALV 913

Query: 920 EQPPLYPHQFFRTLLDLGSVRSM 942
           EQPPLYPHQFFR LLDL S RSM
Sbjct: 914 EQPPLYPHQFFRKLLDLASERSM 936


>Glyma18g44100.1 
          Length = 912

 Score = 1504 bits (3894), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 748/907 (82%), Positives = 805/907 (88%), Gaps = 32/907 (3%)

Query: 53  RATPSIARASAREVVVVDGGVEKTKEEGRVLKVG---------------SARSVLDHLQG 97
           R +   ARA     VV  G VEK +E+GRVLKVG               SARS      G
Sbjct: 21  RRSTRPARAMLHVAVVGGGVVEKGREKGRVLKVGLICGGPSAERGISLNSARS------G 74

Query: 98  DDIHVSCYYIDINLNAFAISSAQVYSNTPADFDFKLESLAQSFSTLDDMAEHLATSVDIV 157
           DD+HVSCYYID NLNAFAISSAQVYSNTPADFDFKLESLAQSF TL D+A+HLAT+VDIV
Sbjct: 75  DDLHVSCYYIDCNLNAFAISSAQVYSNTPADFDFKLESLAQSFQTLSDLAKHLATAVDIV 134

Query: 158 FPVIHGQFGEDGRIQELLEKYNVPYVGTGSKECCQAFDKYKASLELRQHGFVTVPSFLVQ 217
           FPVIHGQFGEDG IQELLEKYNVP+VGTGSKECCQAFDK+KASLELR+HGF+TVPSFLVQ
Sbjct: 135 FPVIHGQFGEDGGIQELLEKYNVPFVGTGSKECCQAFDKHKASLELRKHGFITVPSFLVQ 194

Query: 218 GHKTDKSELSEWFRKHQLDPDLGKVVVKPTRGGSSIGVGVAYGVNDSLVKANEIMTEGID 277
           G++T KSELSEWF KHQLDPDLGKVVVKPTRGGSSIGV VAYGVNDSLVKANEIM+EGID
Sbjct: 195 GYETKKSELSEWFEKHQLDPDLGKVVVKPTRGGSSIGVRVAYGVNDSLVKANEIMSEGID 254

Query: 278 NKVLIEIFLEGGSEFTAIVLDVGSGSDCCPVVLLPTEVELQFLGANDVKENDAIFNYRRK 337
           NKVLIEI+LEGGSEFTAIVLDVGS SD  PVVLLPTEVELQF GANDVKENDAIFNYRRK
Sbjct: 255 NKVLIEIYLEGGSEFTAIVLDVGSASDSFPVVLLPTEVELQFRGANDVKENDAIFNYRRK 314

Query: 338 YLPTQQVAYHTPPRFPLDVIDNIRKGASLIFQRLCLQDFARIDGWFLPNSDSKLSCS-EN 396
           YLPTQQVAYHTPPRFPLDVI+NIRKGASLIFQ+LCLQDFARIDGWFLPNS SKLS S E+
Sbjct: 315 YLPTQQVAYHTPPRFPLDVIENIRKGASLIFQQLCLQDFARIDGWFLPNSSSKLSPSPES 374

Query: 397 EFGRTEAGTILFTDINLISGMEQTSFLFQQASKVGFSHKNILRSVVRHACLRFPNLPSES 456
           EFGRTE+GTI+FTDINLISGMEQTSFLFQQASKVGFSH NILRS++ HACLRFPNL S S
Sbjct: 375 EFGRTESGTIIFTDINLISGMEQTSFLFQQASKVGFSHTNILRSIIHHACLRFPNLASVS 434

Query: 457 GISAQVPTRSKSFELNKAFPFPEGARKVFVIFGGDTSERQVSLMSGTNVWLNLLAFHDLE 516
           GIS Q+P+RSKS + +K+F   EG RKVFVIFGG+TSERQVSLMSGTNVWLNLLAFHDLE
Sbjct: 435 GISGQLPSRSKSLQQSKSFSRHEGTRKVFVIFGGNTSERQVSLMSGTNVWLNLLAFHDLE 494

Query: 517 VTPFLLASTSECESNVDMVKKADDVRDRTIWSLPYSLVLRHTTEEVLAACKEAIEPDRAA 576
           VTP LL+ TS     +        + D       YSLVLRHTTEEVL AC EAIEP+RAA
Sbjct: 495 VTPCLLSPTS---FQLLAFAFRHSIHDH------YSLVLRHTTEEVLDACMEAIEPERAA 545

Query: 577 LTSNLRKKVVNELMEGLKDHNWFTGFDIADELPMKFSLRLWIKSAKEVQATVFIAVHGGI 636
           +TS+LRKKV+N+LMEGLKDHNWFTGFDIAD+LP KFSLR WIK AKEVQAT+FIAVHGGI
Sbjct: 546 ITSDLRKKVMNDLMEGLKDHNWFTGFDIADDLPAKFSLRQWIKLAKEVQATIFIAVHGGI 605

Query: 637 GEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVALKHLANFGVLTINKDVRRKDDLF 696
           GEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVA+KHLAN GVLTINK+VR+KDDL 
Sbjct: 606 GEDGTLQSLLDAEGVPYTGPGAMASKICMDKVATSVAIKHLANSGVLTINKNVRQKDDLS 665

Query: 697 DKPITDIWHDLTQKLQCETLCVKPARDGCSTGVARLCCSDDLAIYVKALEDCLLRIPPNS 756
           +KPI+D WHDLT+KLQC+TLCVKPA+DGCSTGVARLCCS+DLAIYV+ALEDCLLRIPPNS
Sbjct: 666 NKPISDTWHDLTRKLQCQTLCVKPAKDGCSTGVARLCCSEDLAIYVRALEDCLLRIPPNS 725

Query: 757 FSKAHSIIEMPNPPPELLIFEPFIETDEIVVTSKFKE-TGHDLMWKGHSRWVEITVGVIG 815
            SKAH +IEMPNPPPE LIFEPFIETDEI+VTSKF++ TGH L WKGHSRWVEITVGVIG
Sbjct: 726 LSKAHGMIEMPNPPPEYLIFEPFIETDEIIVTSKFEDATGHGLTWKGHSRWVEITVGVIG 785

Query: 816 KRGSMHSLSPSVTVKETGDILSLEEKFQGGTGINLTPPPLSIMSEKALQRCKKHIEQIAN 875
           KRGSMHSLSPSVTVKE+GDILSLEEKFQGGTGINLTPPPLSIMSE AL+RCK+HIE IAN
Sbjct: 786 KRGSMHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIMSENALKRCKQHIELIAN 845

Query: 876 TLQLEGFSRIDAFVNVDNGEVLIIEINTVPGMTPSTVLIHQALAEQPPLYPHQFFRTLLD 935
           TLQLEGFSRIDAFVNVD+GEVLIIE+NTVPGMTPSTVLIHQAL EQPPLYPHQFFR LLD
Sbjct: 846 TLQLEGFSRIDAFVNVDSGEVLIIEVNTVPGMTPSTVLIHQALVEQPPLYPHQFFRKLLD 905

Query: 936 LGSVRSM 942
           L S RSM
Sbjct: 906 LASERSM 912


>Glyma16g22350.1 
          Length = 310

 Score = 98.2 bits (243), Expect = 4e-20,   Method: Compositional matrix adjust.
 Identities = 87/231 (37%), Positives = 103/231 (44%), Gaps = 69/231 (29%)

Query: 289 GSEFTAIVLDVGSGSDCCPVVLLPTEVELQFLGANDVKENDAIFNYRRKYLPTQQVAYHT 348
           GSE   IVLDVG  SD   VVLL TE   Q                   YL T +  +  
Sbjct: 1   GSELATIVLDVGFDSDSFLVVLLLTEKMTQ-------------------YLITTRSTFQ- 40

Query: 349 PPRFPLDVIDNIRKGASLIFQRLCLQDFARIDGWFLPNSDSKLSCSEN-EFGRTEAGTIL 407
                           S++FQ LC QDF RIDGWFLPNS SKL  S   EFGR E+G I+
Sbjct: 41  ----------------SMLFQWLCTQDFERIDGWFLPNSSSKLLSSSKSEFGRIESGVII 84

Query: 408 FTDINLISGMEQTSFLFQQASKVG-FSHKNILRSVVRHACLRFPNLPSESGISAQVPTRS 466
           FTD NLIS MEQ  FLFQQASK   +S  N++  +V +  + F    +   I++      
Sbjct: 85  FTDTNLISSMEQIGFLFQQASKFRIWSPSNLIHELVVYQ-VSFQAGQNPCHITSHFLAMK 143

Query: 467 KSFELNKAFPFPEGARKVFVIFGGDTSERQVSLMSGTNVWLNLLAFHDLEV 517
              EL K   F E                           LNLLAFHD+ V
Sbjct: 144 ---ELEKCLSFLE---------------------------LNLLAFHDVSV 164


>Glyma16g22330.1 
          Length = 55

 Score = 90.5 bits (223), Expect = 9e-18,   Method: Compositional matrix adjust.
 Identities = 41/55 (74%), Positives = 47/55 (85%)

Query: 142 TLDDMAEHLATSVDIVFPVIHGQFGEDGRIQELLEKYNVPYVGTGSKECCQAFDK 196
           TL D+A+HLAT+V+IVF + HGQFGED  IQEL E YNVP+VGTGSKE CQAFDK
Sbjct: 1   TLSDLAKHLATTVEIVFLIRHGQFGEDADIQELSETYNVPFVGTGSKESCQAFDK 55