Miyakogusa Predicted Gene

Lj1g3v1465200.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v1465200.1 Non Chatacterized Hit- tr|I1N7W1|I1N7W1_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.27482
PE,29.51,0.000000000005,OS09G0509400 PROTEIN,NULL; FAMILY NOT
NAMED,NULL; seg,NULL; DUF4210,Domain of unknown function
DUF42,CUFF.27351.1
         (499 letters)

Database: Glyma1.pep 
           75,778 sequences; 25,431,882 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Glyma04g37640.2                                                       580   e-166
Glyma04g37640.1                                                       580   e-166
Glyma04g37640.3                                                       503   e-142
Glyma06g17450.1                                                       229   4e-60
Glyma16g05600.1                                                       176   8e-44
Glyma19g26870.2                                                        69   8e-12
Glyma19g26870.1                                                        69   1e-11

>Glyma04g37640.2 
          Length = 665

 Score =  580 bits (1496), Expect = e-166,   Method: Compositional matrix adjust.
 Identities = 317/503 (63%), Positives = 359/503 (71%), Gaps = 28/503 (5%)

Query: 1   MGLPQVSSGCIAEEVAAS---FVQTAPRIASISXXXXXXXXXXXXRCNRTKIHMPHSERN 57
           MGLPQV+SGC AEEVAAS   FVQTAPR+ASI               N   I +  SE+ 
Sbjct: 1   MGLPQVASGCTAEEVAASLGTFVQTAPRMASIGSYEVNLLAGEDL-GNCMHIDVLKSEKK 59

Query: 58  NVIELSK-SHVSKTSKDGKSNIQKLKIDSMEQIARLSVNTE---QTPTSRTVGFRIRAST 113
           NV+ELSK +H+S    D K     LK D MEQI++L VN+E   QTP SRTVGF+IRAST
Sbjct: 60  NVLELSKETHISSMCSDSK-----LKNDPMEQISKLFVNSEKTRQTPVSRTVGFQIRAST 114

Query: 114 PCVNGFGGNGYSSSVFNATNDATEASKSQVRKRLLSPLNGMMLADHFRGDPLDIGEGNHR 173
           P VNGFGGNGYSS VFN  +DATEAS+SQ+RK+LLSPLNGM+LADHF+GDPLDIG G ++
Sbjct: 115 PRVNGFGGNGYSSPVFNVISDATEASESQLRKQLLSPLNGMLLADHFKGDPLDIGAGIYQ 174

Query: 174 TCPKVGDDCHNAIHEYKKVHIGDDINIQPTIWSSAYFQEFVNSSCNDSGMNKNKIVXXXX 233
           +  K GDD  NA+HEYKK HIG++  I   IWS++ FQEF NS CNDS +  N IV    
Sbjct: 175 SRSKAGDDNSNALHEYKKDHIGNNNQIHSMIWSTSCFQEFTNSFCNDSTI--NHIV---- 228

Query: 234 XXXXXXXXXMCYKHLKSSSELNDSKERTKLKSLPVALSIPQKKVSSPRFPLSPLGKKSST 293
                      YKH KSS   N S+E TK +S   ALSIPQK VSSP FPLSPLGKKSS 
Sbjct: 229 SSHHQRAEPWSYKHFKSSPVRNVSEETTKTRSQTAALSIPQKNVSSPPFPLSPLGKKSSK 288

Query: 294 NEKLGGCRTIDVMLDDGNLSFKDVEESLDKTCQGF--------KSQLYYNSMQQKSDMFT 345
           NE LG CR IDV+LDDGN +F  VE+SLD+TCQG         KSQL  N+MQQKSD+FT
Sbjct: 289 NENLGECRNIDVILDDGNFTFNSVEQSLDRTCQGILSAQEIPGKSQLNLNNMQQKSDLFT 348

Query: 346 RDKIIDMNDYWTRPASFPPKHANFCGTMSRLPSRRSLIXXXXXXXXXXXXXXXKVSQKIE 405
            D +IDM +YWT   SFPP+ A  CGTMSRLP RRSL+               KVS KIE
Sbjct: 349 PDNMIDMKEYWTHSGSFPPRDAKLCGTMSRLPIRRSLVGSFEESLLSGRLLSGKVSPKIE 408

Query: 406 GFLAVLNVTGGNFSPQSQKVPFGVSSVQGDKYLLYYSSINLSGK-LSSKSRVSKFQRTLS 464
           GFLA+LNVTGGNFSPQSQK+PF V+SV GDKYLLYYSSINLSGK LS KSRV+KFQRTLS
Sbjct: 409 GFLALLNVTGGNFSPQSQKIPFAVTSVDGDKYLLYYSSINLSGKLLSGKSRVAKFQRTLS 468

Query: 465 MDESRSEKSRIRIPMKGRIQLVL 487
           MDESRSEK RIRIPMKGRIQLVL
Sbjct: 469 MDESRSEKPRIRIPMKGRIQLVL 491


>Glyma04g37640.1 
          Length = 665

 Score =  580 bits (1496), Expect = e-166,   Method: Compositional matrix adjust.
 Identities = 317/503 (63%), Positives = 359/503 (71%), Gaps = 28/503 (5%)

Query: 1   MGLPQVSSGCIAEEVAAS---FVQTAPRIASISXXXXXXXXXXXXRCNRTKIHMPHSERN 57
           MGLPQV+SGC AEEVAAS   FVQTAPR+ASI               N   I +  SE+ 
Sbjct: 1   MGLPQVASGCTAEEVAASLGTFVQTAPRMASIGSYEVNLLAGEDL-GNCMHIDVLKSEKK 59

Query: 58  NVIELSK-SHVSKTSKDGKSNIQKLKIDSMEQIARLSVNTE---QTPTSRTVGFRIRAST 113
           NV+ELSK +H+S    D K     LK D MEQI++L VN+E   QTP SRTVGF+IRAST
Sbjct: 60  NVLELSKETHISSMCSDSK-----LKNDPMEQISKLFVNSEKTRQTPVSRTVGFQIRAST 114

Query: 114 PCVNGFGGNGYSSSVFNATNDATEASKSQVRKRLLSPLNGMMLADHFRGDPLDIGEGNHR 173
           P VNGFGGNGYSS VFN  +DATEAS+SQ+RK+LLSPLNGM+LADHF+GDPLDIG G ++
Sbjct: 115 PRVNGFGGNGYSSPVFNVISDATEASESQLRKQLLSPLNGMLLADHFKGDPLDIGAGIYQ 174

Query: 174 TCPKVGDDCHNAIHEYKKVHIGDDINIQPTIWSSAYFQEFVNSSCNDSGMNKNKIVXXXX 233
           +  K GDD  NA+HEYKK HIG++  I   IWS++ FQEF NS CNDS +  N IV    
Sbjct: 175 SRSKAGDDNSNALHEYKKDHIGNNNQIHSMIWSTSCFQEFTNSFCNDSTI--NHIV---- 228

Query: 234 XXXXXXXXXMCYKHLKSSSELNDSKERTKLKSLPVALSIPQKKVSSPRFPLSPLGKKSST 293
                      YKH KSS   N S+E TK +S   ALSIPQK VSSP FPLSPLGKKSS 
Sbjct: 229 SSHHQRAEPWSYKHFKSSPVRNVSEETTKTRSQTAALSIPQKNVSSPPFPLSPLGKKSSK 288

Query: 294 NEKLGGCRTIDVMLDDGNLSFKDVEESLDKTCQGF--------KSQLYYNSMQQKSDMFT 345
           NE LG CR IDV+LDDGN +F  VE+SLD+TCQG         KSQL  N+MQQKSD+FT
Sbjct: 289 NENLGECRNIDVILDDGNFTFNSVEQSLDRTCQGILSAQEIPGKSQLNLNNMQQKSDLFT 348

Query: 346 RDKIIDMNDYWTRPASFPPKHANFCGTMSRLPSRRSLIXXXXXXXXXXXXXXXKVSQKIE 405
            D +IDM +YWT   SFPP+ A  CGTMSRLP RRSL+               KVS KIE
Sbjct: 349 PDNMIDMKEYWTHSGSFPPRDAKLCGTMSRLPIRRSLVGSFEESLLSGRLLSGKVSPKIE 408

Query: 406 GFLAVLNVTGGNFSPQSQKVPFGVSSVQGDKYLLYYSSINLSGK-LSSKSRVSKFQRTLS 464
           GFLA+LNVTGGNFSPQSQK+PF V+SV GDKYLLYYSSINLSGK LS KSRV+KFQRTLS
Sbjct: 409 GFLALLNVTGGNFSPQSQKIPFAVTSVDGDKYLLYYSSINLSGKLLSGKSRVAKFQRTLS 468

Query: 465 MDESRSEKSRIRIPMKGRIQLVL 487
           MDESRSEK RIRIPMKGRIQLVL
Sbjct: 469 MDESRSEKPRIRIPMKGRIQLVL 491


>Glyma04g37640.3 
          Length = 632

 Score =  503 bits (1295), Expect = e-142,   Method: Compositional matrix adjust.
 Identities = 287/502 (57%), Positives = 327/502 (65%), Gaps = 59/502 (11%)

Query: 1   MGLPQVSSGCIAEEVAAS---FVQTAPRIASISXXXXXXXXXXXXRCNRTKIHMPHSERN 57
           MGLPQV+SGC AEEVAAS   FVQTAPR+ASI               N   I +  SE+ 
Sbjct: 1   MGLPQVASGCTAEEVAASLGTFVQTAPRMASIGSYEVNLLAGEDLG-NCMHIDVLKSEKK 59

Query: 58  NVIELSK-SHVSKTSKDGKSNIQKLKIDSMEQIARLSVNTE---QTPTSRTVGFRIRAST 113
           NV+ELSK +H+S    D K     LK D MEQI++L VN+E   QTP SRTVGF+IRAST
Sbjct: 60  NVLELSKETHISSMCSDSK-----LKNDPMEQISKLFVNSEKTRQTPVSRTVGFQIRAST 114

Query: 114 PCVNGFGGNGYSSSVFNATNDATEASKSQVRKRLLSPLNGMMLADHFRGDPLDIGEGNHR 173
           P VNGFGGNGYSS VFN  +DATEAS+SQ+RK+LLSPLNGM+LADHF+GDPLDIG G ++
Sbjct: 115 PRVNGFGGNGYSSPVFNVISDATEASESQLRKQLLSPLNGMLLADHFKGDPLDIGAGIYQ 174

Query: 174 TCPKVGDDCHNAIHEYKKVHIGDDINIQPTIWSSAYFQEFVNSSCNDSGMNKNKIVXXXX 233
           +  K GDD  NA+HEYKK HIG++  I   IWS++ FQEF NS CNDS +  N IV    
Sbjct: 175 SRSKAGDDNSNALHEYKKDHIGNNNQIHSMIWSTSCFQEFTNSFCNDSTI--NHIV---- 228

Query: 234 XXXXXXXXXMCYKHLKSSSELNDSKERTKLKSLPVALSIPQKKVSSPRFPLSPLGKKSST 293
                      YKH KSS   N S+E TK +S   ALSIPQK VSSP FPLSPLGKKSS 
Sbjct: 229 SSHHQRAEPWSYKHFKSSPVRNVSEETTKTRSQTAALSIPQKNVSSPPFPLSPLGKKSSK 288

Query: 294 NEKLGGCRTIDVMLDDGNLSFKDVEESLDKTCQGF--------KSQLYYNSMQQKSDMFT 345
           NE LG CR IDV+LDDGN +F  VE+SLD+TCQG         KSQL  N+MQQKSD+FT
Sbjct: 289 NENLGECRNIDVILDDGNFTFNSVEQSLDRTCQGILSAQEIPGKSQLNLNNMQQKSDLFT 348

Query: 346 RDKIIDMNDYWTRPASFPPKHANFCGTMSRLPSRRSLIXXXXXXXXXXXXXXXKVSQKIE 405
            D +IDM +YWT   SFPP+ A  CGTMSRLP RRSL+               KVS KIE
Sbjct: 349 PDNMIDMKEYWTHSGSFPPRDAKLCGTMSRLPIRRSLVGSFEESLLSGRLLSGKVSPKIE 408

Query: 406 GFLAVLNVTGGNFSPQSQKVPFGVSSVQGDKYLLYYSSINLSGKLSSKSRVSKFQRTLSM 465
           GFLA+LNVTG                                  LS KSRV+KFQRTLSM
Sbjct: 409 GFLALLNVTG--------------------------------KLLSGKSRVAKFQRTLSM 436

Query: 466 DESRSEKSRIRIPMKGRIQLVL 487
           DESRSEK RIRIPMKGRIQLVL
Sbjct: 437 DESRSEKPRIRIPMKGRIQLVL 458


>Glyma06g17450.1 
          Length = 559

 Score =  229 bits (585), Expect = 4e-60,   Method: Compositional matrix adjust.
 Identities = 120/177 (67%), Positives = 131/177 (74%), Gaps = 9/177 (5%)

Query: 320 SLDKTCQGFKS--------QLYYNSMQQKSDMFTRDKIIDMNDYWTRPASFPPKHANFCG 371
           SLD+TCQG  S        QL  N+MQQKSD+FT D +I+M +YWT   SFPP  A   G
Sbjct: 189 SLDRTCQGISSAQEIPSTSQLNLNNMQQKSDLFTPDNMINMKEYWTHSGSFPPWDAKLRG 248

Query: 372 TMSRLPSRRSLIXXXXXXXXXXXXXXXKVSQKIEGFLAVLNVTGGNFSPQSQKVPFGVSS 431
           TMSRLP RRSL+               KVSQKIEGFLA LNVTGGNFSPQSQK+PF V+S
Sbjct: 249 TMSRLPIRRSLVGSFEESLLSGRLLSGKVSQKIEGFLAFLNVTGGNFSPQSQKIPFAVTS 308

Query: 432 VQGDKYLLYYSSINLSGK-LSSKSRVSKFQRTLSMDESRSEKSRIRIPMKGRIQLVL 487
           V GDKYLLYYSSINLSGK LS KSRV+KFQRTLSMDESRSEK  IR+PMKGRIQLVL
Sbjct: 309 VDGDKYLLYYSSINLSGKLLSGKSRVAKFQRTLSMDESRSEKPHIRVPMKGRIQLVL 365



 Score =  110 bits (276), Expect = 3e-24,   Method: Compositional matrix adjust.
 Identities = 50/83 (60%), Positives = 64/83 (77%)

Query: 145 KRLLSPLNGMMLADHFRGDPLDIGEGNHRTCPKVGDDCHNAIHEYKKVHIGDDINIQPTI 204
           K+LLSPLNGM+LADHF+GDPLDIG G +++  K GD   NA+HEYKK HIG++  I   I
Sbjct: 78  KQLLSPLNGMLLADHFKGDPLDIGAGIYQSRSKAGDHNSNALHEYKKDHIGNNNKIHSMI 137

Query: 205 WSSAYFQEFVNSSCNDSGMNKNK 227
           W+++ FQEF NSSCND+ +N  K
Sbjct: 138 WTTSCFQEFTNSSCNDNTINHIK 160


>Glyma16g05600.1 
          Length = 761

 Score =  176 bits (445), Expect = 8e-44,   Method: Compositional matrix adjust.
 Identities = 175/523 (33%), Positives = 253/523 (48%), Gaps = 48/523 (9%)

Query: 1   MGLPQVSSGCIAEEVA--ASFVQTAPRIASISXXXXXXXXXXXX------RCNRTKIHMP 52
           MGLPQ+     AE+VA   + + ++P+ +  S                      +    P
Sbjct: 1   MGLPQIPPNETAEKVALVGAALTSSPQFSDASPSAIGSMNSVNTGGLAGCTLGSSFDDFP 60

Query: 53  HSERNNVIELSKSHVSKTSKDGKSNIQKLKIDSMEQ---IARLSVNTEQTPTSRTVGF-- 107
            +    +  +S S   + + +  SN+  LKI S ++    A  S      P SR VGF  
Sbjct: 61  KNASLEISNVSDSTFYRGAMEVTSNVHSLKIGSADRSTPFAPKSGRNVHIPASRVVGFES 120

Query: 108 -RIRASTPCVNGFGGNGYSSSVF-NATNDATEASKSQVRKRLLSPLNGMMLADHFRGDPL 165
            R   ST  +         SSVF N   + TE++ S VRKRLLSPL+ M+   HF+GDPL
Sbjct: 121 GRTNTSTDGLTDVSAVNLHSSVFTNFPANDTESASSLVRKRLLSPLSSMLSPSHFKGDPL 180

Query: 166 DIGEGNHRTCPKV-GDDCHNAI-HEYKKVHIGDDINIQPTIWS--SAYFQEFVNSSCNDS 221
           DIG  ++ T   V  D+  N+I  + KK +IG   +   + WS  S   Q  V  S    
Sbjct: 181 DIGCRSNETESLVKNDNFRNSIAQDNKKANIGSKSSYTVSSWSLTSCLEQRTVPHSTESF 240

Query: 222 GMNKNKIVXXXXXXXXXXXXXMCYKHLKSSSELNDSKERTKLKSLPVALSIPQKKVSSPR 281
             +   +                  H    SEL+  + ++ +      +SI  K VSSP 
Sbjct: 241 FQSDGPLKENRGLLSQGSLPTAGIDHF---SELSQVRPQSGI------ISISPKTVSSP- 290

Query: 282 FPLSPLGKKSSTNEK-LGGCRTIDVMLDDGNLSFKDVEESLDKT-------CQGFKSQLY 333
             LSPLG+K S   K  GGCR +   +++ N++ + +E+SLD +       C+   S++ 
Sbjct: 291 LSLSPLGRKFSERIKTAGGCRNVVEEINNWNITSRSIEQSLDNSNSRLMSNCKDDNSRVA 350

Query: 334 YNSMQQKSDMF------TRDKIIDMNDYWTR-PASFPPKHA-NFCGTMSRLPSRRSLIXX 385
             S  +   +       + D I +M+  W     S P  H+  F  ++S L  RRSL+  
Sbjct: 351 RKSFDEVEFLCKDFCPSSLDDIAEMS--WPLCQESAPTSHSMRFTRSLSGLSVRRSLVGS 408

Query: 386 XXXXXXXXXXXXXKVSQKIEGFLAVLNVTGGNFSPQSQKVPFGVSSVQGDKYLLYYSSIN 445
                          S+KI+GFLAVL++TGGNFSP+SQK+PF V+SV GD YLLYY+SI+
Sbjct: 409 FEESLLSGRFLSGNNSKKIDGFLAVLSITGGNFSPKSQKLPFSVTSVDGDCYLLYYASID 468

Query: 446 LSGKLSS-KSRVSKFQRTLSMDESRSEKSRIRIPMKGRIQLVL 487
           L+G  SS K R    +R    D+S+  KSR+RIPMKGRIQLVL
Sbjct: 469 LAGNSSSNKLRGQLLKRGFGKDDSQIVKSRLRIPMKGRIQLVL 511


>Glyma19g26870.2 
          Length = 663

 Score = 69.3 bits (168), Expect = 8e-12,   Method: Compositional matrix adjust.
 Identities = 95/344 (27%), Positives = 146/344 (42%), Gaps = 30/344 (8%)

Query: 1   MGLPQVSSGCIAEEVA--ASFVQTAPRIASISXXXXXXXXXXXX------RCNRTKIHMP 52
           MGLPQ+     AE+VA   + + ++P  +  S                      +    P
Sbjct: 1   MGLPQIPPNETAEKVALVGAALTSSPHFSDASPSAINSLNSVNTGGLAGCTLGSSFDDFP 60

Query: 53  HSERNNVIELSKSHVSKTSKDGKSNIQKLKIDSMEQ---IARLSVNTEQTPTSRTVGFRI 109
            +    +  +S     + + +  SN+  LKI S ++    A  S      P  R VGF  
Sbjct: 61  KNASLEISNVSDDTFYRGAMEVASNVHSLKIGSTDKSTPFAPKSGRNVHIPALRVVGFES 120

Query: 110 RASTPCVNGFGG----NGYSSSVFNATNDATEASKSQVRKRLLSPLNGMMLADHFRGDPL 165
             +    +G       N +SS   N   +  E++ S VRKRLLSPL+ ++   HF+GDPL
Sbjct: 121 GQTNTSTDGLADVSVVNLHSSVSTNFPANDNESASSLVRKRLLSPLSSILSPSHFKGDPL 180

Query: 166 DIGEGNHRTCPKV-GDDCHNAI-HEYKKVHIGDDINIQPTIWS--SAYFQEFVNSSCNDS 221
           DIG  N+ T   V  D+  N+I H+ KK +IG   +   + WS  +   Q  V  S    
Sbjct: 181 DIGCRNNETGSLVKNDNVRNSIAHDNKKANIGSKSSYTMSSWSLTNCLEQRIVPHSTESF 240

Query: 222 GMNKNKIVXXXXXXXXXXXXXMCYKHLKSSSELNDSKERTKLKSLPVALSIPQKKVSSPR 281
             +   +                  H    SEL+  + +  +       SI  K VSSP 
Sbjct: 241 FQSDGPLKENRGLLSQGSSPTAGIDHF---SELSQVRPQNGI------TSISPKNVSSP- 290

Query: 282 FPLSPLGKKSSTNEK-LGGCRTIDVMLDDGNLSFKDVEESLDKT 324
             LSPLG+K S   K  GGCR +   + + N++ + +E+SLD +
Sbjct: 291 LSLSPLGRKFSERIKTAGGCRNVVEEIKNCNITSRSIEQSLDNS 334


>Glyma19g26870.1 
          Length = 673

 Score = 69.3 bits (168), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 95/344 (27%), Positives = 146/344 (42%), Gaps = 30/344 (8%)

Query: 1   MGLPQVSSGCIAEEVA--ASFVQTAPRIASISXXXXXXXXXXXX------RCNRTKIHMP 52
           MGLPQ+     AE+VA   + + ++P  +  S                      +    P
Sbjct: 1   MGLPQIPPNETAEKVALVGAALTSSPHFSDASPSAINSLNSVNTGGLAGCTLGSSFDDFP 60

Query: 53  HSERNNVIELSKSHVSKTSKDGKSNIQKLKIDSMEQ---IARLSVNTEQTPTSRTVGFRI 109
            +    +  +S     + + +  SN+  LKI S ++    A  S      P  R VGF  
Sbjct: 61  KNASLEISNVSDDTFYRGAMEVASNVHSLKIGSTDKSTPFAPKSGRNVHIPALRVVGFES 120

Query: 110 RASTPCVNGFGG----NGYSSSVFNATNDATEASKSQVRKRLLSPLNGMMLADHFRGDPL 165
             +    +G       N +SS   N   +  E++ S VRKRLLSPL+ ++   HF+GDPL
Sbjct: 121 GQTNTSTDGLADVSVVNLHSSVSTNFPANDNESASSLVRKRLLSPLSSILSPSHFKGDPL 180

Query: 166 DIGEGNHRTCPKV-GDDCHNAI-HEYKKVHIGDDINIQPTIWS--SAYFQEFVNSSCNDS 221
           DIG  N+ T   V  D+  N+I H+ KK +IG   +   + WS  +   Q  V  S    
Sbjct: 181 DIGCRNNETGSLVKNDNVRNSIAHDNKKANIGSKSSYTMSSWSLTNCLEQRIVPHSTESF 240

Query: 222 GMNKNKIVXXXXXXXXXXXXXMCYKHLKSSSELNDSKERTKLKSLPVALSIPQKKVSSPR 281
             +   +                  H    SEL+  + +  +       SI  K VSSP 
Sbjct: 241 FQSDGPLKENRGLLSQGSSPTAGIDHF---SELSQVRPQNGIT------SISPKNVSSP- 290

Query: 282 FPLSPLGKKSSTNEK-LGGCRTIDVMLDDGNLSFKDVEESLDKT 324
             LSPLG+K S   K  GGCR +   + + N++ + +E+SLD +
Sbjct: 291 LSLSPLGRKFSERIKTAGGCRNVVEEIKNCNITSRSIEQSLDNS 334