Miyakogusa Predicted Gene
- Lj1g3v0102560.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj1g3v0102560.1 Non Chatacterized Hit- tr|I1NGH9|I1NGH9_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.398
PE=4,78.57,0.000000000000003,Homocysteine
S-methyltransferase,Homocysteine S-methyltransferase;
S-methyl_trans,Homocysteine S-met,gene.g28835.t1.1
(263 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma20g28720.6 378 e-105
Glyma20g28720.5 377 e-105
Glyma20g28720.1 377 e-105
Glyma20g28720.4 377 e-105
Glyma20g28720.3 377 e-105
Glyma19g34120.1 284 8e-77
Glyma03g31280.1 241 5e-64
Glyma08g30150.1 231 4e-61
Glyma20g28720.2 211 6e-55
Glyma08g30150.2 170 2e-42
Glyma05g23960.1 83 3e-16
Glyma20g28690.1 78 1e-14
Glyma02g35470.1 60 3e-09
>Glyma20g28720.6
Length = 280
Score = 378 bits (971), Expect = e-105, Method: Compositional matrix adjust.
Identities = 184/215 (85%), Positives = 194/215 (90%)
Query: 1 MRDFLDKCGGYAVIDGGFATELERHGADLHDPLWSAKCLISSPHLVRRVHLDYLDAGANI 60
MRDFLDKCGG AVIDGGFATELERHGADL+D LWSAKCLISSPHLVRRVHLDYLDAGANI
Sbjct: 12 MRDFLDKCGGCAVIDGGFATELERHGADLNDELWSAKCLISSPHLVRRVHLDYLDAGANI 71
Query: 61 ILTASYQATLQGFEAKGFSREEGETLLRRSVELALEAREIYNARCTKDSSDFIRDERYRR 120
ILTASYQAT+QGFEAKGFSREEGET+LRRSVE+A EAREIY RCTKDSSDF+RDERYR+
Sbjct: 72 ILTASYQATIQGFEAKGFSREEGETMLRRSVEIAREAREIYYDRCTKDSSDFMRDERYRK 131
Query: 121 RPILVAASVGSYGAYLADGAEYTGDYGDAVTVQTLKDFHRERVKILVDAGADLIAFETIP 180
RPIL+AASVGSYGAYLADG+EY GDYGDAVTVQTLKDFHRERVKILV+AGADLIAFETIP
Sbjct: 132 RPILIAASVGSYGAYLADGSEYVGDYGDAVTVQTLKDFHRERVKILVEAGADLIAFETIP 191
Query: 181 NKMXXXXXXXXXXXXGIEIPAWLSFSCKDESNVKS 215
NK+ GIE PAW SFSCKDESNV S
Sbjct: 192 NKLEAQAYAELLEEEGIETPAWFSFSCKDESNVVS 226
>Glyma20g28720.5
Length = 298
Score = 377 bits (969), Expect = e-105, Method: Compositional matrix adjust.
Identities = 184/215 (85%), Positives = 194/215 (90%)
Query: 1 MRDFLDKCGGYAVIDGGFATELERHGADLHDPLWSAKCLISSPHLVRRVHLDYLDAGANI 60
MRDFLDKCGG AVIDGGFATELERHGADL+D LWSAKCLISSPHLVRRVHLDYLDAGANI
Sbjct: 12 MRDFLDKCGGCAVIDGGFATELERHGADLNDELWSAKCLISSPHLVRRVHLDYLDAGANI 71
Query: 61 ILTASYQATLQGFEAKGFSREEGETLLRRSVELALEAREIYNARCTKDSSDFIRDERYRR 120
ILTASYQAT+QGFEAKGFSREEGET+LRRSVE+A EAREIY RCTKDSSDF+RDERYR+
Sbjct: 72 ILTASYQATIQGFEAKGFSREEGETMLRRSVEIAREAREIYYDRCTKDSSDFMRDERYRK 131
Query: 121 RPILVAASVGSYGAYLADGAEYTGDYGDAVTVQTLKDFHRERVKILVDAGADLIAFETIP 180
RPIL+AASVGSYGAYLADG+EY GDYGDAVTVQTLKDFHRERVKILV+AGADLIAFETIP
Sbjct: 132 RPILIAASVGSYGAYLADGSEYVGDYGDAVTVQTLKDFHRERVKILVEAGADLIAFETIP 191
Query: 181 NKMXXXXXXXXXXXXGIEIPAWLSFSCKDESNVKS 215
NK+ GIE PAW SFSCKDESNV S
Sbjct: 192 NKLEAQAYAELLEEEGIETPAWFSFSCKDESNVVS 226
>Glyma20g28720.1
Length = 342
Score = 377 bits (968), Expect = e-105, Method: Compositional matrix adjust.
Identities = 184/215 (85%), Positives = 194/215 (90%)
Query: 1 MRDFLDKCGGYAVIDGGFATELERHGADLHDPLWSAKCLISSPHLVRRVHLDYLDAGANI 60
MRDFLDKCGG AVIDGGFATELERHGADL+D LWSAKCLISSPHLVRRVHLDYLDAGANI
Sbjct: 12 MRDFLDKCGGCAVIDGGFATELERHGADLNDELWSAKCLISSPHLVRRVHLDYLDAGANI 71
Query: 61 ILTASYQATLQGFEAKGFSREEGETLLRRSVELALEAREIYNARCTKDSSDFIRDERYRR 120
ILTASYQAT+QGFEAKGFSREEGET+LRRSVE+A EAREIY RCTKDSSDF+RDERYR+
Sbjct: 72 ILTASYQATIQGFEAKGFSREEGETMLRRSVEIAREAREIYYDRCTKDSSDFMRDERYRK 131
Query: 121 RPILVAASVGSYGAYLADGAEYTGDYGDAVTVQTLKDFHRERVKILVDAGADLIAFETIP 180
RPIL+AASVGSYGAYLADG+EY GDYGDAVTVQTLKDFHRERVKILV+AGADLIAFETIP
Sbjct: 132 RPILIAASVGSYGAYLADGSEYVGDYGDAVTVQTLKDFHRERVKILVEAGADLIAFETIP 191
Query: 181 NKMXXXXXXXXXXXXGIEIPAWLSFSCKDESNVKS 215
NK+ GIE PAW SFSCKDESNV S
Sbjct: 192 NKLEAQAYAELLEEEGIETPAWFSFSCKDESNVVS 226
Score = 88.6 bits (218), Expect = 6e-18, Method: Compositional matrix adjust.
Identities = 45/56 (80%), Positives = 46/56 (82%), Gaps = 2/56 (3%)
Query: 210 ESN--VKSSGVAERDFVPYIDKWCDAGASLFGGCCRTTPNTIRGIAEAIYGKPQGK 263
ESN VKSSG AE DFV YI KW DAGASLFGGCCRTTPNTIRGIAEA YGK + K
Sbjct: 285 ESNQWVKSSGAAEHDFVSYIGKWRDAGASLFGGCCRTTPNTIRGIAEATYGKLKDK 340
>Glyma20g28720.4
Length = 340
Score = 377 bits (967), Expect = e-105, Method: Compositional matrix adjust.
Identities = 184/215 (85%), Positives = 194/215 (90%)
Query: 1 MRDFLDKCGGYAVIDGGFATELERHGADLHDPLWSAKCLISSPHLVRRVHLDYLDAGANI 60
MRDFLDKCGG AVIDGGFATELERHGADL+D LWSAKCLISSPHLVRRVHLDYLDAGANI
Sbjct: 12 MRDFLDKCGGCAVIDGGFATELERHGADLNDELWSAKCLISSPHLVRRVHLDYLDAGANI 71
Query: 61 ILTASYQATLQGFEAKGFSREEGETLLRRSVELALEAREIYNARCTKDSSDFIRDERYRR 120
ILTASYQAT+QGFEAKGFSREEGET+LRRSVE+A EAREIY RCTKDSSDF+RDERYR+
Sbjct: 72 ILTASYQATIQGFEAKGFSREEGETMLRRSVEIAREAREIYYDRCTKDSSDFMRDERYRK 131
Query: 121 RPILVAASVGSYGAYLADGAEYTGDYGDAVTVQTLKDFHRERVKILVDAGADLIAFETIP 180
RPIL+AASVGSYGAYLADG+EY GDYGDAVTVQTLKDFHRERVKILV+AGADLIAFETIP
Sbjct: 132 RPILIAASVGSYGAYLADGSEYVGDYGDAVTVQTLKDFHRERVKILVEAGADLIAFETIP 191
Query: 181 NKMXXXXXXXXXXXXGIEIPAWLSFSCKDESNVKS 215
NK+ GIE PAW SFSCKDESNV S
Sbjct: 192 NKLEAQAYAELLEEEGIETPAWFSFSCKDESNVVS 226
Score = 86.7 bits (213), Expect = 2e-17, Method: Compositional matrix adjust.
Identities = 44/55 (80%), Positives = 45/55 (81%), Gaps = 1/55 (1%)
Query: 210 ESNV-KSSGVAERDFVPYIDKWCDAGASLFGGCCRTTPNTIRGIAEAIYGKPQGK 263
ESN KSSG AE DFV YI KW DAGASLFGGCCRTTPNTIRGIAEA YGK + K
Sbjct: 284 ESNQWKSSGAAEHDFVSYIGKWRDAGASLFGGCCRTTPNTIRGIAEATYGKLKDK 338
>Glyma20g28720.3
Length = 341
Score = 377 bits (967), Expect = e-105, Method: Compositional matrix adjust.
Identities = 184/215 (85%), Positives = 194/215 (90%)
Query: 1 MRDFLDKCGGYAVIDGGFATELERHGADLHDPLWSAKCLISSPHLVRRVHLDYLDAGANI 60
MRDFLDKCGG AVIDGGFATELERHGADL+D LWSAKCLISSPHLVRRVHLDYLDAGANI
Sbjct: 12 MRDFLDKCGGCAVIDGGFATELERHGADLNDELWSAKCLISSPHLVRRVHLDYLDAGANI 71
Query: 61 ILTASYQATLQGFEAKGFSREEGETLLRRSVELALEAREIYNARCTKDSSDFIRDERYRR 120
ILTASYQAT+QGFEAKGFSREEGET+LRRSVE+A EAREIY RCTKDSSDF+RDERYR+
Sbjct: 72 ILTASYQATIQGFEAKGFSREEGETMLRRSVEIAREAREIYYDRCTKDSSDFMRDERYRK 131
Query: 121 RPILVAASVGSYGAYLADGAEYTGDYGDAVTVQTLKDFHRERVKILVDAGADLIAFETIP 180
RPIL+AASVGSYGAYLADG+EY GDYGDAVTVQTLKDFHRERVKILV+AGADLIAFETIP
Sbjct: 132 RPILIAASVGSYGAYLADGSEYVGDYGDAVTVQTLKDFHRERVKILVEAGADLIAFETIP 191
Query: 181 NKMXXXXXXXXXXXXGIEIPAWLSFSCKDESNVKS 215
NK+ GIE PAW SFSCKDESNV S
Sbjct: 192 NKLEAQAYAELLEEEGIETPAWFSFSCKDESNVVS 226
Score = 88.6 bits (218), Expect = 6e-18, Method: Compositional matrix adjust.
Identities = 45/56 (80%), Positives = 46/56 (82%), Gaps = 2/56 (3%)
Query: 210 ESN--VKSSGVAERDFVPYIDKWCDAGASLFGGCCRTTPNTIRGIAEAIYGKPQGK 263
ESN VKSSG AE DFV YI KW DAGASLFGGCCRTTPNTIRGIAEA YGK + K
Sbjct: 284 ESNQWVKSSGAAEHDFVSYIGKWRDAGASLFGGCCRTTPNTIRGIAEATYGKLKDK 339
>Glyma19g34120.1
Length = 333
Score = 284 bits (726), Expect = 8e-77, Method: Compositional matrix adjust.
Identities = 143/217 (65%), Positives = 169/217 (77%), Gaps = 2/217 (0%)
Query: 1 MRDFLDKCGGYAVIDGGFATELERHGADLHDPLWSAKCLISSPHLVRRVHLDYLDAGANI 60
+ D L + GG AVIDGG ATELERHGADL+DPLWSAKCL S PHL+R+VHLDYL+ GA+I
Sbjct: 5 ITDLLRQTGGTAVIDGGLATELERHGADLNDPLWSAKCLFSFPHLIRQVHLDYLENGADI 64
Query: 61 ILTASYQATLQGFEAKGFSREEGETLLRRSVELALEAREIYNARCTK-DSSDFIRDER-Y 118
I+TASYQAT+QGF+AKG+S EE E LLR SVE+A EARE+Y C S D D R
Sbjct: 65 IITASYQATIQGFKAKGYSDEESEALLRSSVEIAREAREVYYKNCAGCRSGDGDDDGRIL 124
Query: 119 RRRPILVAASVGSYGAYLADGAEYTGDYGDAVTVQTLKDFHRERVKILVDAGADLIAFET 178
++RPILVAASVGSYGAYLADG+EY+GDYGDA+TV+TLKDFHR RV+IL D+GADL+AFET
Sbjct: 125 KQRPILVAASVGSYGAYLADGSEYSGDYGDAITVETLKDFHRRRVQILADSGADLLAFET 184
Query: 179 IPNKMXXXXXXXXXXXXGIEIPAWLSFSCKDESNVKS 215
+PNK+ I+IPAW SF+ KD NV S
Sbjct: 185 VPNKLEAEAYAQLLEEEDIKIPAWFSFNSKDGVNVVS 221
Score = 65.9 bits (159), Expect = 5e-11, Method: Compositional matrix adjust.
Identities = 25/40 (62%), Positives = 34/40 (85%)
Query: 213 VKSSGVAERDFVPYIDKWCDAGASLFGGCCRTTPNTIRGI 252
V+++GV + DF+ Y++KWC+ GASL GGCCRTTP+TIR I
Sbjct: 284 VQNTGVTDEDFISYVNKWCELGASLVGGCCRTTPDTIRKI 323
>Glyma03g31280.1
Length = 307
Score = 241 bits (615), Expect = 5e-64, Method: Compositional matrix adjust.
Identities = 144/299 (48%), Positives = 178/299 (59%), Gaps = 53/299 (17%)
Query: 1 MRDFLDKCGGYAVIDGGFATELERHGADLHDPLWSAKCLISSPHLVRRVHLDYLDAGANI 60
+ D L + GG AVIDGG ATELERHG DL+DPLWSAKCL SSPHL+R+VHLDYL+ GA+I
Sbjct: 5 ITDLLRETGGSAVIDGGLATELERHGDDLNDPLWSAKCLFSSPHLIRQVHLDYLENGADI 64
Query: 61 ILTASYQATLQGFEAKGFSREEGETLLRRSVELALEAREIYNARCTK-DSSDFIRDER-Y 118
I+TASYQAT+QGF+AKG+S EE E LL+RS E+A EARE+Y+ C S D D R
Sbjct: 65 IITASYQATIQGFKAKGYSDEESEALLKRSAEIAFEAREVYDKNCAGCCSGDGEDDGRIL 124
Query: 119 RRRPILVAASVGSYGAYLADGAEYTGDYGDAVTVQTLKDFHRERVKILVDAGADLIAFET 178
++RPILVAA VGSYGAYLADG+ Y+GDYGDA+TV+ R RV+IL D+ ADL+AFET
Sbjct: 125 KQRPILVAALVGSYGAYLADGSGYSGDYGDAITVEI-----RRRVQILADSSADLLAFET 179
Query: 179 IPNKMXXXXXXXXXXXXGIEIPAWLSFSCKDESNVKSSG--------------------- 217
+ NK+ I+IPA S + ++ SG
Sbjct: 180 VSNKLEAEAFAQLLEEEDIKIPA-CGDSLMECGSIAESGNKVVAVGIYCTPPRFIHGLIV 238
Query: 218 ----VAERDFVPY----------IDKWCDAGASLF----------GGCCRTTPNTIRGI 252
V + V Y + +W D A GCCRTTP+TIR I
Sbjct: 239 LLKRVTTKPIVIYPNSGETYDADLKEWVDRQAYFVFKIEERSIIECGCCRTTPDTIRKI 297
>Glyma08g30150.1
Length = 323
Score = 231 bits (590), Expect = 4e-61, Method: Compositional matrix adjust.
Identities = 116/229 (50%), Positives = 164/229 (71%), Gaps = 8/229 (3%)
Query: 1 MRDFLDKCGGYAVIDGGFATELERHGADLHDPLWSAKCLISSPHLVRRVHLDYLDAGANI 60
+ D ++ GG AV DGGFAT+LE+HGA ++DPLWSA LI PHL+++VHL+YL+AGA+I
Sbjct: 6 LHDLIENAGGCAVTDGGFATQLEKHGASINDPLWSAIYLIKDPHLIKQVHLEYLEAGADI 65
Query: 61 ILTASYQATLQGFEAKGFSREEGETLLRRSVELALEAREIYNARCTKDSSDFIRDERYRR 120
++T+SYQATL GF +KG S EEGE+LL +SV+LA+EAR+ + +S+ +YRR
Sbjct: 66 LVTSSYQATLPGFSSKGLSIEEGESLLEKSVKLAVEARDGF-----WNSAIINPGNKYRR 120
Query: 121 RPILVAASVGSYGAYLADGAEYTGDYGDAVTVQTLKDFHRERVKILVDAGADLIAFETIP 180
LVAAS+GSYG+YLADG+EY+G YG V ++ LKDFHR R+++LV+AG DL+AFETIP
Sbjct: 121 A--LVAASIGSYGSYLADGSEYSGCYGPDVNLKKLKDFHRRRLQVLVEAGPDLLAFETIP 178
Query: 181 NKMXXXXXXXXXXXXGIEIPAWLSFSCKDESNVKSSGVAERDFVPYIDK 229
NK+ ++IP+W+ F+ D N SG + +D + ++K
Sbjct: 179 NKLEAQACVELLEEESVKIPSWICFTTVDGENA-PSGESFKDCLEALNK 226
>Glyma20g28720.2
Length = 245
Score = 211 bits (537), Expect = 6e-55, Method: Compositional matrix adjust.
Identities = 103/130 (79%), Positives = 111/130 (85%)
Query: 86 LLRRSVELALEAREIYNARCTKDSSDFIRDERYRRRPILVAASVGSYGAYLADGAEYTGD 145
+LRRSVE+A EAREIY RCTKDSSDF+RDERYR+RPIL+AASVGSYGAYLADG+EY GD
Sbjct: 1 MLRRSVEIAREAREIYYDRCTKDSSDFMRDERYRKRPILIAASVGSYGAYLADGSEYVGD 60
Query: 146 YGDAVTVQTLKDFHRERVKILVDAGADLIAFETIPNKMXXXXXXXXXXXXGIEIPAWLSF 205
YGDAVTVQTLKDFHRERVKILV+AGADLIAFETIPNK+ GIE PAW SF
Sbjct: 61 YGDAVTVQTLKDFHRERVKILVEAGADLIAFETIPNKLEAQAYAELLEEEGIETPAWFSF 120
Query: 206 SCKDESNVKS 215
SCKDESNV S
Sbjct: 121 SCKDESNVVS 130
Score = 88.6 bits (218), Expect = 6e-18, Method: Compositional matrix adjust.
Identities = 45/56 (80%), Positives = 46/56 (82%), Gaps = 2/56 (3%)
Query: 210 ESN--VKSSGVAERDFVPYIDKWCDAGASLFGGCCRTTPNTIRGIAEAIYGKPQGK 263
ESN VKSSG AE DFV YI KW DAGASLFGGCCRTTPNTIRGIAEA YGK + K
Sbjct: 188 ESNQWVKSSGAAEHDFVSYIGKWRDAGASLFGGCCRTTPNTIRGIAEATYGKLKDK 243
>Glyma08g30150.2
Length = 278
Score = 170 bits (430), Expect = 2e-42, Method: Compositional matrix adjust.
Identities = 89/183 (48%), Positives = 128/183 (69%), Gaps = 8/183 (4%)
Query: 47 RRVHLDYLDAGANIILTASYQATLQGFEAKGFSREEGETLLRRSVELALEAREIYNARCT 106
+VHL+YL+AGA+I++T+SYQATL GF +KG S EEGE+LL +SV+LA+EAR+ +
Sbjct: 7 NQVHLEYLEAGADILVTSSYQATLPGFSSKGLSIEEGESLLEKSVKLAVEARDGF----- 61
Query: 107 KDSSDFIRDERYRRRPILVAASVGSYGAYLADGAEYTGDYGDAVTVQTLKDFHRERVKIL 166
+S+ +YRR LVAAS+GSYG+YLADG+EY+G YG V ++ LKDFHR R+++L
Sbjct: 62 WNSAIINPGNKYRR--ALVAASIGSYGSYLADGSEYSGCYGPDVNLKKLKDFHRRRLQVL 119
Query: 167 VDAGADLIAFETIPNKMXXXXXXXXXXXXGIEIPAWLSFSCKDESNVKSSGVAERDFVPY 226
V+AG DL+AFETIPNK+ ++IP+W+ F+ D N SG + +D +
Sbjct: 120 VEAGPDLLAFETIPNKLEAQACVELLEEESVKIPSWICFTTVDGENA-PSGESFKDCLEA 178
Query: 227 IDK 229
++K
Sbjct: 179 LNK 181
>Glyma05g23960.1
Length = 92
Score = 83.2 bits (204), Expect = 3e-16, Method: Compositional matrix adjust.
Identities = 39/51 (76%), Positives = 43/51 (84%)
Query: 213 VKSSGVAERDFVPYIDKWCDAGASLFGGCCRTTPNTIRGIAEAIYGKPQGK 263
VKSSGVAE DF+ +I KW D GASLFGGCC+TTPNTIRGIAEA YGK + K
Sbjct: 24 VKSSGVAEDDFISFIGKWRDVGASLFGGCCKTTPNTIRGIAEATYGKLKDK 74
>Glyma20g28690.1
Length = 125
Score = 77.8 bits (190), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 38/60 (63%), Positives = 41/60 (68%)
Query: 156 KDFHRERVKILVDAGADLIAFETIPNKMXXXXXXXXXXXXGIEIPAWLSFSCKDESNVKS 215
K F E+VKILV+AGADLIAFETIPNK+ GIE PAW SFSC DESNV S
Sbjct: 19 KMFLMEKVKILVEAGADLIAFETIPNKLEAQACAELLEEEGIETPAWFSFSCNDESNVVS 78
>Glyma02g35470.1
Length = 132
Score = 59.7 bits (143), Expect = 3e-09, Method: Compositional matrix adjust.
Identities = 29/63 (46%), Positives = 39/63 (61%), Gaps = 5/63 (7%)
Query: 144 GDYGDAVTVQTLKDFHRERVKILVDAGADLIAFETIPNKMXXXXXXXXXXXXGIEIPAWL 203
GDYGDA+TV+ R RV+IL ++ A L+AFET+PNK+ I+IPAW
Sbjct: 1 GDYGDAITVEI-----RRRVQILANSSAHLLAFETVPNKLEAEAFAQLLEEEDIKIPAWF 55
Query: 204 SFS 206
SF+
Sbjct: 56 SFN 58