Miyakogusa Predicted Gene

Lj0g3v0362709.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0362709.1 Non Chatacterized Hit- tr|I3S5I7|I3S5I7_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,100,0,TRP_SYNTHASE_BETA,Tryptophan synthase, beta chain,
conserved site; Tryptophan synthase beta
subunit-,NODE_60639_length_1598_cov_14.050689.path1.1
         (417 letters)

Database: Glyma1.pep 
           75,778 sequences; 25,431,882 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Glyma02g25300.1                                                       763   0.0  
Glyma18g12090.1                                                       755   0.0  
Glyma11g00580.1                                                       508   e-144
Glyma09g15270.1                                                       486   e-137
Glyma09g21940.1                                                       227   2e-59
Glyma10g14640.1                                                       153   3e-37
Glyma18g11650.1                                                       140   4e-33
Glyma13g07130.1                                                       123   4e-28
Glyma14g02830.1                                                        75   9e-14
Glyma19g05630.1                                                        62   9e-10

>Glyma02g25300.1 
          Length = 479

 Score =  763 bits (1971), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 366/417 (87%), Positives = 395/417 (94%), Gaps = 5/417 (1%)

Query: 1   MEDRTKQDSEETNGSSVLSHRPDSFGRYGRFGGKYVPETLMHAITELEAAFHSLAHDEAF 60
           +E+ +K D    NGS VL  RPDSFGR+G+FGGKYVPETLMHA+TELEAAF+SL+ DE F
Sbjct: 68  LEEHSKMD----NGS-VLLQRPDSFGRFGKFGGKYVPETLMHALTELEAAFYSLSADEEF 122

Query: 61  QKELGGILRDYVGRESPLYFAERLTEHYRRGDGEGPHVYLKREDLNHTGAHKINNAVAQA 120
           QKEL GIL+DYVGRESPLYFAERLTEHY+R +GEGPH+YLKREDLNHTGAHKINNAVAQA
Sbjct: 123 QKELAGILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQA 182

Query: 121 LLAKRLGKKRVIAETGAGQHGVATATVCARFGLECVVYMGAQDMERQSLNVFRMRLLGAE 180
           LLAKRLGKKR+IAETGAGQHGVATATVCARFGLEC++YMGAQDMERQ+LNVFRMRLLGAE
Sbjct: 183 LLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAE 242

Query: 181 VRAVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFQAVIGKETRKQ 240
           VR VHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREF AVIGKETRKQ
Sbjct: 243 VRPVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFHAVIGKETRKQ 302

Query: 241 ALEKWGGKPDILVACVGGGSNAMGLFHEFIDDKDVRLIGVEAAGLGLESGKHAATLTKGE 300
           ALEKWGGKPDIL+ACVGGGSNAMGLF+EF+DDKDVRLIGVEAAG GL+SGKHAATLTKGE
Sbjct: 303 ALEKWGGKPDILIACVGGGSNAMGLFNEFVDDKDVRLIGVEAAGFGLDSGKHAATLTKGE 362

Query: 301 IGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDVGRAEYYSVTDEEALEA 360
           +GVLHGAMSYLLQDDDGQI+EPHSISAGLDYPGVGPEHSFLKD+GRAEY+S+TDEEALEA
Sbjct: 363 VGVLHGAMSYLLQDDDGQIVEPHSISAGLDYPGVGPEHSFLKDLGRAEYHSITDEEALEA 422

Query: 361 FKRLSQLEGIIPALETSHALAYLEKLCPTLPNGTKVVVNCSGRGDKDVQTASKYFNV 417
           FKR+S+LEGIIPALETSHALAYLEK+CPTLPNG KVVVN SGRGDKDVQTA K   +
Sbjct: 423 FKRVSRLEGIIPALETSHALAYLEKVCPTLPNGAKVVVNFSGRGDKDVQTAIKCLKL 479


>Glyma18g12090.1 
          Length = 471

 Score =  755 bits (1950), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 356/411 (86%), Positives = 389/411 (94%)

Query: 7   QDSEETNGSSVLSHRPDSFGRYGRFGGKYVPETLMHAITELEAAFHSLAHDEAFQKELGG 66
           ++ ++    SVL  RPDSFGR+G+FGGKYVPETLMHA+++LEAAF+SLA DE FQ EL G
Sbjct: 61  EEHDKMGNGSVLLQRPDSFGRFGKFGGKYVPETLMHALSQLEAAFYSLAADEEFQTELAG 120

Query: 67  ILRDYVGRESPLYFAERLTEHYRRGDGEGPHVYLKREDLNHTGAHKINNAVAQALLAKRL 126
           IL+DYVGRESPLYFAERLTEHY+R +GEGPH+YLKREDLNHTGAHKINNAVAQALLAKRL
Sbjct: 121 ILKDYVGRESPLYFAERLTEHYKRPNGEGPHIYLKREDLNHTGAHKINNAVAQALLAKRL 180

Query: 127 GKKRVIAETGAGQHGVATATVCARFGLECVVYMGAQDMERQSLNVFRMRLLGAEVRAVHS 186
           GKKR+IAETGAGQHGVATATVCARFGLEC++YMGAQDMERQ+LNVFRMRLLGAEVR VHS
Sbjct: 181 GKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVHS 240

Query: 187 GTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFQAVIGKETRKQALEKWG 246
           GTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREF AVIGKETRKQALEKWG
Sbjct: 241 GTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFHAVIGKETRKQALEKWG 300

Query: 247 GKPDILVACVGGGSNAMGLFHEFIDDKDVRLIGVEAAGLGLESGKHAATLTKGEIGVLHG 306
           GKPD+L+ACVGGGSNA+GLF+EF+DDKDVRLIGVEAAG GL+SGKHAATLTKGE+GVLHG
Sbjct: 301 GKPDVLIACVGGGSNAIGLFNEFVDDKDVRLIGVEAAGFGLDSGKHAATLTKGEVGVLHG 360

Query: 307 AMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDVGRAEYYSVTDEEALEAFKRLSQ 366
           AMSYLLQDDDGQI+EPHSISAGLDYPGVGPEHSFLKD+GRAEY+S+TDEEALE FKR+S+
Sbjct: 361 AMSYLLQDDDGQIVEPHSISAGLDYPGVGPEHSFLKDLGRAEYHSITDEEALEGFKRVSR 420

Query: 367 LEGIIPALETSHALAYLEKLCPTLPNGTKVVVNCSGRGDKDVQTASKYFNV 417
           LEGIIPALETSHALAYLEK+CPTL NG KVVVN SGRGDKDVQTA KY  +
Sbjct: 421 LEGIIPALETSHALAYLEKVCPTLANGAKVVVNFSGRGDKDVQTAIKYLKL 471


>Glyma11g00580.1 
          Length = 462

 Score =  508 bits (1309), Expect = e-144,   Method: Compositional matrix adjust.
 Identities = 252/392 (64%), Positives = 312/392 (79%), Gaps = 7/392 (1%)

Query: 26  GRYGRFGGKYVPETLMHAITELEAAFHSLAHDEAFQKELGGILRDYVGRESPLYFAERLT 85
           G++GRFGGK+VPETL+ ++T+LEA F +   D AFQ EL   LRDY GRE+PLY A+RL+
Sbjct: 72  GKFGRFGGKFVPETLVASLTQLEAEFKNALRDHAFQAELAEALRDYAGRETPLYHAKRLS 131

Query: 86  EHYRR-GDGEGPHVYLKREDLNHTGAHKINNAVAQALLAKRLGKKRVIAETGAGQHGVAT 144
           E+Y+   +G+GP +YLKREDLNH+G+HK+NNA+AQA++AKR+G K V+  TG+GQHG+AT
Sbjct: 132 EYYKSVNNGKGPDIYLKREDLNHSGSHKMNNALAQAMIAKRMGCKSVVTATGSGQHGLAT 191

Query: 145 ATVCARFGLECVVYMGAQDMERQSLNVFRMRLLGAEVRAVHSGTATLKDATSEAIRDWVT 204
           A  CA+  L+C V+M  +D+ RQ  NV  ++LLGA+V AV  G     DA SEA R WV 
Sbjct: 192 AAACAKLALDCTVFMADKDIHRQYSNVRLIKLLGAQVEAVDGG---FTDAASEAFRYWVG 248

Query: 205 NVETTHYILGSVAGPHPYPMMVREFQAVIGKETRKQALEKWGGKPDILVACVGGGSNAMG 264
           ++E  +++ GS  GPHP P MVREFQ+VIGKETR QALEKWGGKPD+LVA VG GSNA+G
Sbjct: 249 DLENRYHLSGSAVGPHPCPTMVREFQSVIGKETRMQALEKWGGKPDVLVASVGTGSNALG 308

Query: 265 LFHEFIDDKDVRLIGVEAAGLGLESGKHAATLTKGEIGVLHGAMSYLLQDDDGQIIEPHS 324
           LFHEFI DKDVR +GVEA GLGLESGKH++TLT GE+G  HGA+SYLLQD DGQII PHS
Sbjct: 309 LFHEFIADKDVRFVGVEAGGLGLESGKHSSTLTTGEVGAYHGAISYLLQDQDGQIIHPHS 368

Query: 325 ISAGLDYPGVGPEHSFLKDVGRAEYYSVTDEEALEAFKRLSQLEGIIPALETSHALAYLE 384
           I+AG++YPGVGPE SFLK+ GRAE+    DEEAL+A++RL +LEGI P+LE +HALA L+
Sbjct: 369 IAAGMEYPGVGPELSFLKESGRAEFCVANDEEALDAYQRLCKLEGIFPSLEAAHALAILD 428

Query: 385 KLCPTLPNGTKVVVNCSGRGDKDVQTASKYFN 416
           KL PTL NGTKVVVNCSGRGDKD   A+  FN
Sbjct: 429 KLVPTLCNGTKVVVNCSGRGDKD---AAIVFN 457


>Glyma09g15270.1 
          Length = 359

 Score =  486 bits (1251), Expect = e-137,   Method: Compositional matrix adjust.
 Identities = 258/393 (65%), Positives = 290/393 (73%), Gaps = 37/393 (9%)

Query: 21  RPDSFGRYGRFGGKYVPETLMHAITELEAAFHSLAHDEAFQKELGGILRDYVGRESPLYF 80
           RPDSFGR+G+FGGKYVPETLMHA+TELEA F+SL+ DE FQ EL GIL+DYVGRESPLYF
Sbjct: 1   RPDSFGRFGKFGGKYVPETLMHALTELEANFYSLSADEEFQTELAGILKDYVGRESPLYF 60

Query: 81  AERLTEHYRRGDGEGPHVYLKREDLNHTGAHKINNAVAQALLAKRLGKKRVIAETGAGQH 140
           AER +     G+   P +      L      +       ALL K    +RV  E     +
Sbjct: 61  AERASHLSEEGNSITPGLTKLTMLLLKLCLLR--GWAKNALLLKL---ERVSMELPLLLY 115

Query: 141 GVATATVCARFGLECVVYMGAQDMERQSLNVFRMRLLGAEVRAVHSGTATLKDATSEAIR 200
                             +   DMERQ+LNVFRMRLLGAE R  HSGT TLKDATSEAIR
Sbjct: 116 ------------------VLDLDMERQALNVFRMRLLGAEARPFHSGTTTLKDATSEAIR 157

Query: 201 DWVTNVETTHYILGSVAGPHPYPMMVREFQAVIGKETRKQALEKWGGKPDILVACVGGGS 260
           DWVTNV TTHYIL  VA PHPYPMM   F  V G+ETR+             +ACVGGGS
Sbjct: 158 DWVTNVGTTHYILCYVARPHPYPMMKASFGKV-GRETRR-------------IACVGGGS 203

Query: 261 NAMGLFHEFIDDKDVRLIGVEAAGLGLESGKHAATLTKGEIGVLHGAMSYLLQDDDGQII 320
           NAMGLF+EF+DDKDVRLIG+EA G GL+SGKHA TLTKGE+GVLHGAMSYLLQDDDG+I+
Sbjct: 204 NAMGLFNEFVDDKDVRLIGMEAVGFGLDSGKHATTLTKGEVGVLHGAMSYLLQDDDGEIV 263

Query: 321 EPHSISAGLDYPGVGPEHSFLKDVGRAEYYSVTDEEALEAFKRLSQLEGIIPALETSHAL 380
           EPHSISAGLDY GVG +HSF+KD+GRAEY+S+T EEALEAFKR+S+LEGIIPALETSHAL
Sbjct: 264 EPHSISAGLDYLGVGSKHSFMKDLGRAEYHSITYEEALEAFKRVSRLEGIIPALETSHAL 323

Query: 381 AYLEKLCPTLPNGTKVVVNCSGRGDKDVQTASK 413
           AYLEK+CPTLPNG KVVVN SGRGDKDVQTA K
Sbjct: 324 AYLEKVCPTLPNGAKVVVNFSGRGDKDVQTAIK 356


>Glyma09g21940.1 
          Length = 326

 Score =  227 bits (578), Expect = 2e-59,   Method: Compositional matrix adjust.
 Identities = 145/359 (40%), Positives = 197/359 (54%), Gaps = 61/359 (16%)

Query: 39  TLMHAITELEAAFHSLAHDEAFQKELGGILRDYVGRESPLYFAERLTEHYRRGDGEGPHV 98
           TL+   ++LEA F+   HD+ FQ EL   LRDYVGRE+PLY +E                
Sbjct: 1   TLVACSSQLEAEFNKALHDDVFQAELVVALRDYVGRETPLYLSECW-------------- 46

Query: 99  YLKREDLNHTGAHKINNAVAQALLAKRLGKKRVIAETGAGQHGVATATVCARFGLECVVY 158
               ++LNH+G+HK+NN +AQA++AKR+G  R         H   T   C    +    +
Sbjct: 47  ----KNLNHSGSHKMNNVLAQAMIAKRVGCHR---------HWFGTTWPCHSCSMRKTCF 93

Query: 159 MGAQDMERQSLNVFRMRLLGAEVRAVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAG 218
                 +RQS  V  ++LLGA+V    S    L+  T   IR   ++  T+ +  GS   
Sbjct: 94  A-----DRQSSKVRLIKLLGAQVLIEASKIHHLRHFT---IR--FSSGTTSMFDHGSQVS 143

Query: 219 PHPYPMMVREFQAVIGKETRKQALEKWGGKPDILVACVGGGSNAMGLFHEFIDDKDVRLI 278
                         +GKE R           D+LV CVG GSNA+GLFHEFI   DVRLI
Sbjct: 144 VDDRERNKDSSIGKMGKEAR-----------DVLVTCVGIGSNAIGLFHEFIAHSDVRLI 192

Query: 279 GVEAAGLGLESGKHAATLTKGEIGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEH 338
            VE  GLGLESG+H++ LT GE+G+ HG+MSYLLQDDDGQ+IEPHSI  G+         
Sbjct: 193 RVETGGLGLESGRHSSKLTMGEVGIYHGSMSYLLQDDDGQVIEPHSIIDGV--------- 243

Query: 339 SFLKDVGRAEYYSVTDEEALEAFKRLSQLEGIIPALETSHALAYLEKLCPTLPNGTKVV 397
                +  +  + +   +A + +KRLS+LEG+  +LE +HALA L+KL PTL NG++VV
Sbjct: 244 ----SMSWSRKFVLQMIKAFDEYKRLSKLEGLFLSLEAAHALAILDKLVPTLCNGSQVV 298


>Glyma10g14640.1 
          Length = 122

 Score =  153 bits (387), Expect = 3e-37,   Method: Compositional matrix adjust.
 Identities = 83/151 (54%), Positives = 100/151 (66%), Gaps = 29/151 (19%)

Query: 30  RFGGKYVPETLMHAITELEAAFHSLAHDEAFQKELGGILRDYVGRESPLYFAERLTEHYR 89
           RFG KYV ETL+HA+TELE+ FHSLA DE F+  L   LR+YVGR+S L FA+RL   Y 
Sbjct: 1   RFGSKYVLETLIHALTELESVFHSLASDEHFK--LAKTLREYVGRKSLLCFAKRLMRRYA 58

Query: 90  RGDGEGPHVYLKREDLNHTGAHKINNAVAQALLAKRLGKKRVIAETGAGQHGVATATVCA 149
              G G                            +RLGK+RVIAETG GQHGVATATVC 
Sbjct: 59  CKSGGGL---------------------------ERLGKRRVIAETGVGQHGVATATVCE 91

Query: 150 RFGLECVVYMGAQDMERQSLNVFRMRLLGAE 180
           RFGLECV+Y+GA++ME+ +LNVF+MRLLGA+
Sbjct: 92  RFGLECVMYVGARNMEKHALNVFKMRLLGAK 122


>Glyma18g11650.1 
          Length = 95

 Score =  140 bits (352), Expect = 4e-33,   Method: Compositional matrix adjust.
 Identities = 82/135 (60%), Positives = 84/135 (62%), Gaps = 40/135 (29%)

Query: 282 AAGLGLESGKHAATLTKGEIGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFL 341
           AAGLGLES          + GVL G+MSYLLQDDDG           LDYPGVG EHSF 
Sbjct: 1   AAGLGLES----------KAGVLDGSMSYLLQDDDG-----------LDYPGVGLEHSFS 39

Query: 342 KDVGRAEYYSVTDEEALEAFKRLSQLEGIIPALETSHALAYLEKLCPTLPNGTKVVVNCS 401
           KD                   RLSQLEGI  ALETSHALAYLEK CPTLPN TKVVVNCS
Sbjct: 40  KD-------------------RLSQLEGITAALETSHALAYLEKFCPTLPNVTKVVVNCS 80

Query: 402 GRGDKDVQTASKYFN 416
           GRGDKDVQT  KY N
Sbjct: 81  GRGDKDVQTTLKYLN 95


>Glyma13g07130.1 
          Length = 502

 Score =  123 bits (308), Expect = 4e-28,   Method: Compositional matrix adjust.
 Identities = 107/333 (32%), Positives = 150/333 (45%), Gaps = 45/333 (13%)

Query: 74  RESPLYFAERLTEHYRRGDGEGPHVYLKREDLNHTGAHKINNAVAQALLAKRLGKKRVIA 133
           R +PL  A+RL +           +Y K E ++  G+HK N+AV QA    + G K V+ 
Sbjct: 127 RPTPLIRAKRLEKLLDTPA----RIYYKYEGVSPAGSHKPNSAVPQAWYNLQAGVKNVVT 182

Query: 134 ETGAGQHGVATATVCARFGLECVVYMGAQDMERQSLNVFRMRLLGAEVRAV--------- 184
           ETGAGQ G A A  C+ FGL C V+      + +      M+  GA+V            
Sbjct: 183 ETGAGQWGSALAFACSIFGLGCEVWQVRASYDSKPYRRLMMQTWGAKVHPSPSMITEAGR 242

Query: 185 ------HSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFQAVIGKETR 238
                  S   +L  A SEA+     N + T Y LGSV         V   Q+VIG+E  
Sbjct: 243 RMLQEDPSSPGSLGIAISEAVEVAAKNAD-TKYCLGSVLN------HVLLHQSVIGEECI 295

Query: 239 KQALEKWGGKPDILVACVGGGSNAMGLFHEFIDDKDVRLI-----GVEAAGLGLESGKHA 293
           KQ +E  G  PD+++ C GGGSN  GL   F+ +K  + I      VE A          
Sbjct: 296 KQ-MEAIGETPDVIIGCTGGGSNFAGLSFPFLREKLNKKINPVIRAVEPAA--------C 346

Query: 294 ATLTKG----EIGVLHGAMSYLLQDDDGQIIEPHSISA-GLDYPGVGPEHSFLKDVGRAE 348
            +LTKG    + G   G    +     G    P  I A GL Y G+ P  S + D+G  E
Sbjct: 347 PSLTKGVYTYDYGDTAGMTPLMKMHTLGHDFVPDPIHAGGLRYHGMAPLISHVYDLGLME 406

Query: 349 YYSVTDEEALEAFKRLSQLEGIIPALETSHALA 381
             ++   E  +   + ++ EG+IPA E +HA+A
Sbjct: 407 AIAIPQTECFQGAIQFARSEGLIPAPEPTHAIA 439


>Glyma14g02830.1 
          Length = 167

 Score = 75.5 bits (184), Expect = 9e-14,   Method: Compositional matrix adjust.
 Identities = 38/72 (52%), Positives = 45/72 (62%), Gaps = 19/72 (26%)

Query: 200 RDWVTNVETTHYILGSVAGPHPYPMMVREFQAVIGKETR-------------------KQ 240
           RD + N+ETTH I+ SVAGP+PYP+MVREF AVI KETR                   KQ
Sbjct: 83  RDRMANLETTHNIMDSVAGPYPYPVMVREFHAVIDKETRKQALEKCIRNQMCCLDNCKKQ 142

Query: 241 ALEKWGGKPDIL 252
           ALEKW  +PD+L
Sbjct: 143 ALEKWIDEPDVL 154


>Glyma19g05630.1 
          Length = 241

 Score = 62.4 bits (150), Expect = 9e-10,   Method: Compositional matrix adjust.
 Identities = 61/200 (30%), Positives = 87/200 (43%), Gaps = 23/200 (11%)

Query: 230 QAVIGKETRKQALEKWGGKPDILVACVGGGSNAMGLFHEFIDDKDVRLI-----GVEAAG 284
            +VIG+E  KQ +E  G  PD++  C GGGSN  GL   F+ +K  + I      VE A 
Sbjct: 30  HSVIGEEWIKQ-MEAIGETPDVIKGCTGGGSNFAGLSFPFLREKLNKKINPVIRAVEPAA 88

Query: 285 LGLESGKHAATLTKG----EIGVLHGAMSYLLQDDDGQIIEPHSISA-GLDYPGVGPEHS 339
                     +LTKG    + G   G    +     G    P  I A GL Y G+ P  S
Sbjct: 89  --------CPSLTKGVYTYDYGDTAGMTPLIKMHTLGHDFVPDPIHAGGLRYHGMAPLIS 140

Query: 340 FLKDVGRAEYYSVTDEEALEAFKRLSQLEGIIPALETSHALAYLEK---LCPTLPNGTKV 396
            + D+G  E  ++   E L A  + ++ EG+IPA E +HA+A   +    C        +
Sbjct: 141 HVFDLGLMEAIAIPQTECLGAI-QFARSEGLIPASEPTHAIAATIREAIRCRETGEAKVI 199

Query: 397 VVNCSGRGDKDVQTASKYFN 416
           +    G G  D+    KY  
Sbjct: 200 LTAMCGHGHFDLPAYEKYLQ 219