Miyakogusa Predicted Gene
- Lj0g3v0208009.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj0g3v0208009.1 tr|G7J608|G7J608_MEDTR Neutral ceramidase
OS=Medicago truncatula GN=MTR_3g079190 PE=4
SV=1,84.98,0,CERAMIDASE,Neutral/alkaline nonlysosomal ceramidase;
Ceramidase_alk,Neutral/alkaline nonlysosomal ce,CUFF.13352.1
(408 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma13g03750.1 496 e-140
Glyma08g45100.1 368 e-102
Glyma18g07680.1 362 e-100
Glyma02g47420.1 356 3e-98
Glyma14g01330.1 356 3e-98
Glyma18g07700.1 260 2e-69
Glyma20g10250.1 250 2e-66
Glyma18g07690.1 240 2e-63
>Glyma13g03750.1
Length = 768
Score = 496 bits (1276), Expect = e-140, Method: Compositional matrix adjust.
Identities = 238/293 (81%), Positives = 254/293 (86%)
Query: 41 DIGQLVKKAQSIKATGGKDCNQHTSQASKVRKNDGSLFVGSFCQSNVGDVSPNVLGAFCI 100
+IGQL+KKAQSIKAT GKDC + SQASKVRKNDGSLFVG+FCQSNVGDVSPNVLGAFCI
Sbjct: 295 NIGQLMKKAQSIKATRGKDCKKLASQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCI 354
Query: 101 DTGHPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVDLFESASEELTGNI 160
D+G PCDFN SSC+GNDQLCVGRGPGYPDEILSTKIIGERQFKTAV LFES SEEL+G I
Sbjct: 355 DSGKPCDFNRSSCHGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVKLFESTSEELSGKI 414
Query: 161 DYRHAYLNFTNIEVELDTNKVVKTCXXXXXXXXXXXXXXXXXXXXFQQGDTEINPFLKTV 220
DYRH YLNFT+IEVELD+NKVVKTC FQQGDT+INPF K V
Sbjct: 415 DYRHVYLNFTDIEVELDSNKVVKTCPAALGPGFAAGTTDGPGLFGFQQGDTKINPFWKNV 474
Query: 221 RDTLKEPSQYQVGCQNPKAVLLSTGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTM 280
RD L +PSQYQV CQNPK VLLSTGEMF PYPWAPAILPIQILRLGKLIILSVPGE TTM
Sbjct: 475 RDFLTKPSQYQVDCQNPKPVLLSTGEMFYPYPWAPAILPIQILRLGKLIILSVPGELTTM 534
Query: 281 SGRRLREAVKKTLISSSNGEFNNETHVVVAGLTNTYSQYIATFEEYQQQRYEV 333
+GRRLREAVK+TLISSSNGEF++ETHVV+AGLTNTYSQYIATFEEYQQQRYE
Sbjct: 535 AGRRLREAVKETLISSSNGEFDDETHVVIAGLTNTYSQYIATFEEYQQQRYEA 587
>Glyma08g45100.1
Length = 757
Score = 368 bits (944), Expect = e-102, Method: Compositional matrix adjust.
Identities = 189/354 (53%), Positives = 235/354 (66%), Gaps = 23/354 (6%)
Query: 1 MSRENKLISGDNKGVAARLFEDLFAS-----------QXXXXXXXXXXXVP----DIGQL 45
MSR N LISGDNKG AAR ED F + +P + +L
Sbjct: 223 MSRTNSLISGDNKGAAARFMEDWFEQKDYGRTDSVVFEDDALPRRMSNIIPSHHDNHREL 282
Query: 46 VKKAQSIKATGGKDCNQHTSQASKVR----KNDGSLFVGSFCQSNVGDVSPNVLGAFCID 101
++ A S ++ G+ + +S A +VR K FV +FCQSN GDVSPNVLGAFCID
Sbjct: 283 LELATSFQSPPGRPVTKTSSVAKRVRSAHRKVGKRRFVSAFCQSNCGDVSPNVLGAFCID 342
Query: 102 TGHPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVDLFESASEELTGNID 161
TG PCDFNHS+C G ++LC RGPGYPDE ST+IIGERQF+ AVDLF +A EE+ G++D
Sbjct: 343 TGLPCDFNHSTCGGKNELCYSRGPGYPDEFESTRIIGERQFRKAVDLFNAADEEIEGDVD 402
Query: 162 YRHAYLNFTNIEVELDT---NKVVKTCXXXXXXXXXXXXXXXXXXXXFQQGDTEINPFLK 218
+RHAY++F+ +EV + ++VVKTC FQQGD + NPF K
Sbjct: 403 FRHAYIDFSQLEVTISDQGYSEVVKTCPAAMGFAFAAGTTDGPGAFDFQQGDDKGNPFWK 462
Query: 219 TVRDTLKEPSQYQVGCQNPKAVLLSTGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFT 278
VRD LK PS+ Q+ CQ+PK +LL TGEM PY WAP+ILPIQILR+G+LIILSVPGEFT
Sbjct: 463 LVRDMLKTPSREQIDCQHPKPILLDTGEMKKPYDWAPSILPIQILRIGQLIILSVPGEFT 522
Query: 279 TMSGRRLREAVKKTLISSSNGEFNNETHVVVAGLTNTYSQYIATFEEYQQQRYE 332
TM+GRRLR+AVK L S + EF ++ H+V+AGLTNTYSQYI T+EEYQ QRYE
Sbjct: 523 TMAGRRLRDAVKMVLTSEEDFEF-DDIHIVIAGLTNTYSQYITTYEEYQVQRYE 575
>Glyma18g07680.1
Length = 779
Score = 362 bits (928), Expect = e-100, Method: Compositional matrix adjust.
Identities = 188/354 (53%), Positives = 231/354 (65%), Gaps = 23/354 (6%)
Query: 1 MSRENKLISGDNKGVAARLFEDLFASQXXXXXXXX-----------XXXVP----DIGQL 45
MSR N LISGDNKG AAR ED F + +P + +L
Sbjct: 245 MSRTNSLISGDNKGAAARFMEDWFEQKDYGKTDSVVFEDDVLLRRISNIIPSRHDNHHEL 304
Query: 46 VKKAQSIKATGGKDCNQHTSQASKVR----KNDGSLFVGSFCQSNVGDVSPNVLGAFCID 101
++ A S ++ G+ ++ +S A +VR K D FV +FCQSN GDVSPNVLGAFCID
Sbjct: 305 LELATSFQSPPGRPVSKTSSVAKRVRSAHRKVDKPRFVSAFCQSNCGDVSPNVLGAFCID 364
Query: 102 TGHPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVDLFESASEELTGNID 161
TG PCDFNHS+C G ++LC RGPGYPDE ST+IIGERQF+ AVDLF +A EE+ G +D
Sbjct: 365 TGLPCDFNHSTCGGKNELCYSRGPGYPDEFESTRIIGERQFRKAVDLFNAADEEIEGGVD 424
Query: 162 YRHAYLNFTNIEVELDT---NKVVKTCXXXXXXXXXXXXXXXXXXXXFQQGDTEINPFLK 218
+RHAY++F+ +EV + ++VVKTC FQQGD + NPF K
Sbjct: 425 FRHAYIDFSQLEVTISDQGYSEVVKTCPAAMGFAFAAGTTDGPGAFDFQQGDDKGNPFWK 484
Query: 219 TVRDTLKEPSQYQVGCQNPKAVLLSTGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFT 278
VRD LK PS+ Q CQ PK +LL TGEM PY WA +ILPIQILR+G+LIILSVPG FT
Sbjct: 485 LVRDMLKTPSKEQTDCQRPKPILLDTGEMKKPYDWAASILPIQILRIGQLIILSVPGGFT 544
Query: 279 TMSGRRLREAVKKTLISSSNGEFNNETHVVVAGLTNTYSQYIATFEEYQQQRYE 332
TM+GRRLR+AVK L S EF ++ H+V+AGLTNTYSQYI T+EEYQ QRYE
Sbjct: 545 TMAGRRLRDAVKTVLTSEEYFEF-DDIHIVIAGLTNTYSQYITTYEEYQVQRYE 597
>Glyma02g47420.1
Length = 768
Score = 356 bits (913), Expect = 3e-98, Method: Compositional matrix adjust.
Identities = 183/354 (51%), Positives = 230/354 (64%), Gaps = 24/354 (6%)
Query: 1 MSRENKLISGDNKGVAARLFEDLF---ASQXXXXXXXXXXXVP------------DIGQL 45
MSR N LISGDNKG AAR ED F S VP + +L
Sbjct: 235 MSRTNSLISGDNKGAAARFMEDWFERKGSVRMDLVRFENDGVPRRISNIIPSLHDNYHEL 294
Query: 46 VKKAQSIKATGGKDCNQHTSQASKVR----KNDGSLFVGSFCQSNVGDVSPNVLGAFCID 101
++ A S ++ GK + +S A +VR + D FV +FCQ+N GDVSPNVLG FCID
Sbjct: 295 LELAASFRSPLGKPATKTSSIARRVRGVLRQVDKPRFVSAFCQTNCGDVSPNVLGTFCID 354
Query: 102 TGHPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVDLFESASEELTGNID 161
TG PCDFNHS+C G ++LC GRGPGYPDE ST+IIGERQFK AV+LF ASE++ G +D
Sbjct: 355 TGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFKKAVELFNGASEQIKGKVD 414
Query: 162 YRHAYLNFTNIEVELD---TNKVVKTCXXXXXXXXXXXXXXXXXXXXFQQGDTEINPFLK 218
+RHA+++F+ + V L ++V+KTC F+QGD + NPF K
Sbjct: 415 FRHAFIDFSQLGVNLSKVGASEVIKTCPAAMGFAFAAGTTDGPGAFDFKQGDDQGNPFWK 474
Query: 219 TVRDTLKEPSQYQVGCQNPKAVLLSTGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFT 278
VR+ LK P + Q+ C +PK +LL TGEM PY WAP+ILPIQ+LR+G+L+ILSVPGEFT
Sbjct: 475 LVRNLLKTPGKEQIDCHHPKPILLDTGEMKLPYDWAPSILPIQVLRVGQLVILSVPGEFT 534
Query: 279 TMSGRRLREAVKKTLISSSNGEFNNETHVVVAGLTNTYSQYIATFEEYQQQRYE 332
TM+GRRLR+AVK L S N F + HVV+AGLTNTYSQY+ T+EEYQ QRYE
Sbjct: 535 TMAGRRLRDAVKTVL--SGNKGFGSNIHVVIAGLTNTYSQYVTTYEEYQVQRYE 586
>Glyma14g01330.1
Length = 768
Score = 356 bits (913), Expect = 3e-98, Method: Compositional matrix adjust.
Identities = 184/354 (51%), Positives = 234/354 (66%), Gaps = 24/354 (6%)
Query: 1 MSRENKLISGDNKGVAARLFEDLFASQXXXXXXXX-----------XXXVPDIG----QL 45
MSR N LISGDNKG AAR ED F + +P + +L
Sbjct: 235 MSRTNSLISGDNKGAAARFMEDWFERKGSVRMDSVGFENDGIPRRISNIIPSLHDNHHEL 294
Query: 46 VKKAQSIKATGGKDCNQHTSQASKVR----KNDGSLFVGSFCQSNVGDVSPNVLGAFCID 101
++ A S ++ GK + +S A +VR + D FV +FCQ+N GDVSPNVLGAFCID
Sbjct: 295 LELAASFQSPPGKPATKTSSVARRVRGVLTQVDKPRFVSAFCQTNCGDVSPNVLGAFCID 354
Query: 102 TGHPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVDLFESASEELTGNID 161
T PCDFNHS+C G ++LC GRGPGYPDE ST+IIGERQFK AV+LF ASE++ G +D
Sbjct: 355 TELPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFKKAVELFNGASEQIKGKVD 414
Query: 162 YRHAYLNFTNIEV---ELDTNKVVKTCXXXXXXXXXXXXXXXXXXXXFQQGDTEINPFLK 218
+RHA+++F+ +EV ++ ++VVKTC F+QGD + NPF
Sbjct: 415 FRHAFIDFSQLEVNPSKVGASEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDQGNPFWM 474
Query: 219 TVRDTLKEPSQYQVGCQNPKAVLLSTGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFT 278
VR+ LK P + QV C +PK +LL TGEM PY WAP+ILPIQILR+G+L+ILSVPGEFT
Sbjct: 475 LVRNLLKTPGKEQVDCHHPKPILLDTGEMKLPYDWAPSILPIQILRVGQLVILSVPGEFT 534
Query: 279 TMSGRRLREAVKKTLISSSNGEFNNETHVVVAGLTNTYSQYIATFEEYQQQRYE 332
TM+GRRLR+AV KT++S S G F + HVV+AGLTNTYSQY+ T+EEYQ QRYE
Sbjct: 535 TMAGRRLRDAV-KTVLSGSKG-FGSNIHVVIAGLTNTYSQYVTTYEEYQVQRYE 586
>Glyma18g07700.1
Length = 481
Score = 260 bits (665), Expect = 2e-69, Method: Compositional matrix adjust.
Identities = 132/248 (53%), Positives = 163/248 (65%), Gaps = 8/248 (3%)
Query: 92 PNVLGAFCIDTGHPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVDLFES 151
PNVLGAFCID G PCDFN S C G ++LC +GP YPDE+ ST+IIGERQF+ AVDLF +
Sbjct: 73 PNVLGAFCIDIGLPCDFNRSICGGKNELCYSQGPSYPDELESTRIIGERQFRKAVDLFNA 132
Query: 152 ASEELTGNIDYRHAYLNFTNIEVELDT---NKVVKTCXXXXXXXXXXXXXXXXXXXXFQQ 208
A E+ +D+RHAY++F+ +EV + ++VVKTC F +
Sbjct: 133 ADGEIGRGVDFRHAYIDFSQLEVTISDQGYSEVVKTCPAAMGLHLRLEQQMDLEFLIFSK 192
Query: 209 GDTEINPF----LKTVRDTLKEPSQYQVGCQNPKAVLLSTGEMFDPYPWAPAILPIQILR 264
+ K VRD LK PS+ Q CQ PK +LL TGEM PY WAP+ILPIQILR
Sbjct: 193 VMIRLAVLNISHGKLVRDMLKTPSKEQTDCQCPKPILLDTGEMKKPYDWAPSILPIQILR 252
Query: 265 LGKLIILSVPGEFTTMSGRRLREAVKKTLISSSNGEFNNETHVVVAGLTNTYSQYIATFE 324
+G+LIILSVPGEFTTM+ R LR+ VK L S EF++ H+V+AGLTNTYS I T+E
Sbjct: 253 IGQLIILSVPGEFTTMAKRHLRDIVKTVLTSEEYFEFDD-IHIVIAGLTNTYSHDITTYE 311
Query: 325 EYQQQRYE 332
EYQ QRYE
Sbjct: 312 EYQVQRYE 319
>Glyma20g10250.1
Length = 445
Score = 250 bits (638), Expect = 2e-66, Method: Compositional matrix adjust.
Identities = 153/321 (47%), Positives = 183/321 (57%), Gaps = 81/321 (25%)
Query: 44 QLVKKAQSIKATGGKDCNQHTSQASKVRKNDGSLF----------------VGSFC---Q 84
QLVKKAQSIKA GGKDCN+ SQASKVRKNDGSLF +G+FC +
Sbjct: 1 QLVKKAQSIKAIGGKDCNKQASQASKVRKNDGSLFVGAFCQSNVGDVSPDVLGAFCIDSE 60
Query: 85 SNVGDVSPNVLGAFCIDTGHPCDF---------------NHSSCNGNDQLCVGRGPG--- 126
+ +L + F N+ +G ++ +GR
Sbjct: 61 KSWKCAKEEMLFVLGFKRRNHITFQLMKVAFGQQWRNKNNNGILSGMKKIGIGRVDKHLV 120
Query: 127 ------------------YPDEILSTKIIGERQFKTAVDLFESASEELTGNIDYRH--AY 166
YPDEILSTKIIGERQFKT V+LFES SEEL+G IDY H +
Sbjct: 121 LNCSYILYYFLNFFNISMYPDEILSTKIIGERQFKTVVELFESTSEELSGKIDYNHVNSK 180
Query: 167 LNFTNIEVELDTNKVVKTCXXXXXXXXXXXXXXXXXXXXFQQGDTEINPFLKTVRDTLKE 226
NF I+ + T + +++ Q INPF K VRD LKE
Sbjct: 181 QNFYYIK-DSTTKQFIRS-----------------------QSKHLINPFWKNVRDFLKE 216
Query: 227 PSQYQVGCQNPKAVLLSTGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMSGRRLR 286
PSQ+Q CQ PK VLLSTGEMF PYPWAPAILPIQI+RLGK IILSVPGEF TM+GRRLR
Sbjct: 217 PSQHQEHCQKPKPVLLSTGEMFFPYPWAPAILPIQIIRLGKFIILSVPGEFITMAGRRLR 276
Query: 287 EAVKKTLISSSNGEFNNETHV 307
EAVK+TLISS++GEF++ET+V
Sbjct: 277 EAVKETLISSNDGEFDDETYV 297
>Glyma18g07690.1
Length = 474
Score = 240 bits (613), Expect = 2e-63, Method: Compositional matrix adjust.
Identities = 141/349 (40%), Positives = 179/349 (51%), Gaps = 77/349 (22%)
Query: 93 NVLGAFCIDTGHPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVDLFESA 152
+V FCIDTG PCDFN S+C ++L +GPGYPDE+ +DLF +A
Sbjct: 23 SVAKPFCIDTGLPCDFNRSTCGRKNELVYSQGPGYPDEL-------------EMDLFNAA 69
Query: 153 SEELTGNIDYRHAYLNFTNIEVELDT---NKVVKTCXXXXXXXXXXXXXXXXXXXXFQQG 209
EE+ G++D+RHAY++F+ +E+ + ++VVKTC FQQG
Sbjct: 70 DEEIEGDVDFRHAYIDFSQLELTISDQGYSEVVKTCPATMGFAFSARTTDGPRAFDFQQG 129
Query: 210 DTEINPFLKTVRDTLKEPSQYQVGCQNPKAVLLSTGEMFDPYPWA--------------- 254
D + NPF K VRD LK PS+ Q CQ PK +LL TGEM PY WA
Sbjct: 130 DDKGNPFWKLVRDMLKTPSKEQTDCQCPKPILLDTGEMKKPYDWALALNKYNIVSSWSNL 189
Query: 255 ------------------------------------PAILPIQILRLGKLIILSVPGEFT 278
P+ILPIQILR+G+LIILSVPGEFT
Sbjct: 190 LFWDRLLLESHLNCNHPQVDSLAITHKLIALGLRGSPSILPIQILRIGQLIILSVPGEFT 249
Query: 279 TMSGRRLREAVKKTLISSSNGEFNNETHVVVAGLTNTYSQYIATFEEYQQQRYEVWKRIP 338
TM+GR LR+AVK L S EF ++ H+V+AGLTNTYSQYI T+EEYQ QRYE
Sbjct: 250 TMAGRHLRDAVKTVLTSEEYFEF-DDIHIVIAGLTNTYSQYITTYEEYQVQRYET----- 303
Query: 339 SSIGNLYLEQXXXXXXXXXXXXXCRLLQHYMVLTHYQHTSKNLRDSHKQ 387
N + ++L YMV TH HT +LR+ KQ
Sbjct: 304 ----NHHAHLCAYVPTCTCMWRILQVLLRYMVHTHSGHTFWSLRNLRKQ 348