Miyakogusa Predicted Gene

Lj0g3v0069809.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0069809.1 tr|B9H4K8|B9H4K8_POPTR DNA topoisomerase
OS=Populus trichocarpa GN=POPTRDRAFT_861019 PE=3
SV=1,79.24,0,PRTPISMRASEI,DNA topoisomerase, type IA;
TOPOISOMERASE_I_PROK,DNA topoisomerase, type IA, active
sit,CUFF.3360.1
         (452 letters)

Database: Glyma1.pep 
           75,778 sequences; 25,431,882 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Glyma06g16200.1                                                       417   e-116
Glyma11g36800.1                                                       172   7e-43
Glyma06g16170.1                                                        58   3e-08

>Glyma06g16200.1 
          Length = 603

 Score =  417 bits (1071), Expect = e-116,   Method: Compositional matrix adjust.
 Identities = 197/225 (87%), Positives = 211/225 (93%), Gaps = 2/225 (0%)

Query: 12  LKVAEELYQTGFISYPRTETDSFSPRTNLHAIVQEQQGHPEWGIYAQRLLDPEAGLWRNP 71
           +KVAEELYQ GFISYPRTETDSFSP T+LH IVQEQQGHPEWGIYAQRL+DPEAGLWRNP
Sbjct: 314 MKVAEELYQAGFISYPRTETDSFSPGTDLHTIVQEQQGHPEWGIYAQRLMDPEAGLWRNP 373

Query: 72  RGGGHDDKAHPPIHPTKFSAGESGWSQNHRKLYELVVRHFLACVSKPAVGAETTVEIDIA 131
           RGGGHDDKAHPPI+PTKFS GESGWSQ+HRKLYELVVRHFLACVSKPA+GAETTVEI+IA
Sbjct: 374 RGGGHDDKAHPPIYPTKFSTGESGWSQDHRKLYELVVRHFLACVSKPALGAETTVEINIA 433

Query: 132 GELFSACGRVILEKNYLDVYPFETWGGSLIPTYTVGQQFTPTALTLDSGVTRPPPLLSEA 191
           GELFSACGRVILEKNYLDVY +E+WGGS+IPTYT GQQF PT LTL+SGVTRPPPLLSEA
Sbjct: 434 GELFSACGRVILEKNYLDVYRYESWGGSMIPTYTNGQQFNPTKLTLESGVTRPPPLLSEA 493

Query: 192 DLLSCMDKE--GIGTDATMHDHIKKLLDRFYATKDASTRFTPTNL 234
           DLLS MD+E   IGTDATM DHIKK+LDR YATKD+STRFTPTNL
Sbjct: 494 DLLSYMDREEAKIGTDATMQDHIKKMLDRSYATKDSSTRFTPTNL 538


>Glyma11g36800.1 
          Length = 863

 Score =  172 bits (436), Expect = 7e-43,   Method: Compositional matrix adjust.
 Identities = 104/320 (32%), Positives = 161/320 (50%), Gaps = 15/320 (4%)

Query: 12  LKVAEELYQTGFISYPRTETDSFSPRTNLHAIVQEQQGHPEWGIYAQRLLDPEAGLWRNP 71
           +++AE LY  GFISYPRTE+ ++ P  +   ++  Q  +P WG Y Q LL      ++ P
Sbjct: 314 MQLAERLYTQGFISYPRTESTAYPPSFDFRGVLSAQTNNPTWGNYVQGLL---TNGYQKP 370

Query: 72  RGGGHDDKAHPPIHPTKFSAGESGWSQNHRKLYELVVRHFLACVSKPAVGAETTVEIDIA 131
           R G  D   HPPI P + SA E     +  KLY+ + +HF+  VS         VE  I 
Sbjct: 371 RLGT-DVGDHPPITPMR-SAAEDMLGNDAWKLYQYICQHFIGTVSPDCKYIRRKVEFSIG 428

Query: 132 GELFSACGRVILEKNYLDVYPFETWGGSLIPTYTVGQQFTPTALTLDSGVTRPPPLLSEA 191
           GE F   G+ ++ K +  + P+       IP++  GQ+   + L L  G T PP  L+E+
Sbjct: 429 GESFHCTGQHVVTKGFTAIMPWLAINDKNIPSFIKGQKIEVSKLELYEGSTSPPDFLTES 488

Query: 192 DLLSCMDKEGIGTDATMHDHIKKLLDRFYATKDASTRFTPTNLGEALVMGYDDIEMAYKL 251
           +L+S M+K GIGTDA++  HI  + +R Y    A  +  PT LG  LV GY  I+    L
Sbjct: 489 ELISLMEKNGIGTDASIPVHINNICERNYVQVQAGRKLVPTTLGITLVRGYQTIDP--DL 546

Query: 252 WKPELRSAMESDMKSVSEGTKGKDAVLATSLDKMKACFLHARLNKVKLLEAMSIFFQRSN 311
             P++RS +E  +  +++G      V+   + +    F +     VK +E M   F+   
Sbjct: 547 CLPDIRSFIEQQITLIAKGQVDHRHVVQHVIQQFTQKFSYF----VKKIEDMDALFEAQF 602

Query: 312 RSAGDELHATGEVVRRCGLC 331
            +  D    +G ++ +CG C
Sbjct: 603 STLTD----SGRILSKCGKC 618


>Glyma06g16170.1 
          Length = 43

 Score = 57.8 bits (138), Expect = 3e-08,   Method: Composition-based stats.
 Identities = 26/42 (61%), Positives = 29/42 (69%)

Query: 249 YKLWKPELRSAMESDMKSVSEGTKGKDAVLATSLDKMKACFL 290
           YKLWKP+LRS ME  M  V +G K K  VL T L KM+ACFL
Sbjct: 1   YKLWKPDLRSEMEKKMDDVKKGDKSKAEVLVTILQKMEACFL 42