Miyakogusa Predicted Gene

Lj6g3v2193660.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj6g3v2193660.1 Non Characterized Hit- tr|F6HLD3|F6HLD3_VITVI
Putative uncharacterized protein OS=Vitis vinifera
GN=,73.02,4.2039e-45,Pseudouridine synthase,Pseudouridine synthase,
catalytic domain; DKCLD,Dyskerin-like; CENTROMERE/MIC,CUFF.60886.1
         (581 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g074160.1 | centromere/microtubule-binding protein | HC | ...   768   0.0  
Medtr6g004400.1 | tRNA pseudouridine synthase B | HC | chr6:2658...   102   1e-21
Medtr6g004400.2 | tRNA pseudouridine synthase B | HC | chr6:2658...    64   3e-10

>Medtr4g074160.1 | centromere/microtubule-binding protein | HC |
           chr4:28203993-28206323 | 20130731
          Length = 612

 Score =  768 bits (1983), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 366/400 (91%), Positives = 386/400 (96%), Gaps = 5/400 (1%)

Query: 32  DEPQ----FMIKPEKLTPTIDTSQWPILLKNYDRLNVRTGHYTPLPSGYSPLKRPLPEYL 87
           +EPQ    FMIKPEKLTPTIDTS WPILLKNYDRLNVRTGHYTPLP+G+SPLKRP+ +YL
Sbjct: 26  EEPQNPQDFMIKPEKLTPTIDTSNWPILLKNYDRLNVRTGHYTPLPAGHSPLKRPIADYL 85

Query: 88  KYGVLNLDKPANPSSHEVVAWIKRLLRVEKTGHSGTLDPKVTGNLIVCIDRATRLVKSQQ 147
           KYGV+NLDKPANPSSHEVVAWIKR+LRVEKTGHSGTLDPKVTGNLIVCIDRATRLVKSQQ
Sbjct: 86  KYGVINLDKPANPSSHEVVAWIKRILRVEKTGHSGTLDPKVTGNLIVCIDRATRLVKSQQ 145

Query: 148 GAGKEYVCIARLHSDVPDVSKVARALETLTGAVFQRPPLISAVKRQLRIRTIYESKLLEY 207
           GAGKEYVCIARLHS VPDV+KVARALETLTGAVFQRPPLISAVKRQLRIRTIYESK+LEY
Sbjct: 146 GAGKEYVCIARLHSAVPDVAKVARALETLTGAVFQRPPLISAVKRQLRIRTIYESKMLEY 205

Query: 208 DPDRHLVVFWISCEAGTYVRTMCVHLGLVLGVGAHMQELRRVRSGIMGEKDNMVTMHDVI 267
           D ++HLVVFWISCEAGTYVRTMCVHLGLVLGVG HMQELRRVRSGIMGEKDNMVTMHDV+
Sbjct: 206 DAEKHLVVFWISCEAGTYVRTMCVHLGLVLGVGGHMQELRRVRSGIMGEKDNMVTMHDVM 265

Query: 268 DAQWVFDNYR-DESYLRRVVMPLEVLLTSYKRLVVKDSAVNAICYGAKLMIPGLLRFEND 326
           DAQ+V+D  + DE+YLRRVVMPLEVLLTSYKRLVVKDSAVNAICYGAKLMIPGLLRFEND
Sbjct: 266 DAQYVYDKEKSDETYLRRVVMPLEVLLTSYKRLVVKDSAVNAICYGAKLMIPGLLRFEND 325

Query: 327 IEAGEEVVLMTTKGEAIALGIAEMTTAVMATCDHGVVAKIKRVVMDRDTYPRKWGLGPRA 386
           IE GEEVVLMTTKGEAIALGIAEMTTAVMATCDHG+VAKIKRVVMDRDTYPRKWGLGPRA
Sbjct: 326 IEPGEEVVLMTTKGEAIALGIAEMTTAVMATCDHGIVAKIKRVVMDRDTYPRKWGLGPRA 385

Query: 387 SMKKKLIAAGKLDKHGKPNESTPQEWMRNLVLPAGGDSMI 426
           SMKKKLIAAGKLDKHGKPNESTPQEWMRN+VLP GGD++I
Sbjct: 386 SMKKKLIAAGKLDKHGKPNESTPQEWMRNVVLPTGGDAVI 425


>Medtr6g004400.1 | tRNA pseudouridine synthase B | HC |
           chr6:265898-259538 | 20130731
          Length = 572

 Score =  102 bits (253), Expect = 1e-21,   Method: Compositional matrix adjust.
 Identities = 62/192 (32%), Positives = 99/192 (51%), Gaps = 34/192 (17%)

Query: 91  VLNLDKPANPSSHEVVAWIKRLLRVEKTGHSGTLDPKVTGNLIVCIDRATRLVKSQQGAG 150
           VL ++KP   +S  V   ++RL++V+K GH+GTLDP  TG LIVC+ ++T+LV   QG  
Sbjct: 355 VLLINKPKGWTSFTVCGKLRRLVKVKKVGHAGTLDPMATGLLIVCVGKSTKLVDRYQGMV 414

Query: 151 KEYVCIARL-------HSDVPDVSK----------VARALETLTGAVFQRPPLISAVK-- 191
           K Y  + RL        +D P + +          + ++  +  G ++Q PP+ SA+K  
Sbjct: 415 KGYSGVFRLGEATSTWDADSPVIQREPWEHIKDEDIKKSALSFCGEIWQVPPMFSAIKVG 474

Query: 192 ------RQLRIRTIYES----KLLEYD-----PDRHLVVFWISCEAGTYVRTMCVHLGLV 236
                 +  R  +I  S     + ++D      DR  ++F + C  GTY+R++C   G  
Sbjct: 475 GEKLYEKARRGESIELSPRRISIFQFDIERSLDDRQNLIFRVICSKGTYIRSLCADFGKA 534

Query: 237 LGVGAHMQELRR 248
           LG  AH+  LRR
Sbjct: 535 LGSCAHLTALRR 546


>Medtr6g004400.2 | tRNA pseudouridine synthase B | HC |
           chr6:265862-262540 | 20130731
          Length = 437

 Score = 64.3 bits (155), Expect = 3e-10,   Method: Compositional matrix adjust.
 Identities = 32/69 (46%), Positives = 46/69 (66%)

Query: 91  VLNLDKPANPSSHEVVAWIKRLLRVEKTGHSGTLDPKVTGNLIVCIDRATRLVKSQQGAG 150
           VL ++KP   +S  V   ++RL++V+K GH+GTLDP  TG LIVC+ ++T+LV   QG  
Sbjct: 355 VLLINKPKGWTSFTVCGKLRRLVKVKKVGHAGTLDPMATGLLIVCVGKSTKLVDRYQGMV 414

Query: 151 KEYVCIARL 159
           K Y  + RL
Sbjct: 415 KGYSGVFRL 423