Miyakogusa Predicted Gene

Lj6g3v1392460.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj6g3v1392460.1 Non Characterized Hit- tr|I3SXQ0|I3SXQ0_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,73.49,0,seg,NULL;
MOSC N-terminal domain-like,NULL; PK beta-barrel domain-like,Pyruvate
kinase-like, insert ,CUFF.59459.1
         (331 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr2g035480.1 | Mo-molybdopterin cofactor sulfurase | HC | chr...   484   e-137
Medtr2g035470.1 | Mo-molybdopterin cofactor sulfurase | HC | chr...   473   e-134
Medtr2g035460.1 | Mo-molybdopterin cofactor sulfurase | HC | chr...   473   e-133
Medtr2g035460.2 | Mo-molybdopterin cofactor sulfurase | HC | chr...   423   e-118
Medtr2g035470.2 | Mo-molybdopterin cofactor sulfurase | HC | chr...   356   2e-98
Medtr2g035480.2 | Mo-molybdopterin cofactor sulfurase | HC | chr...   356   2e-98
Medtr2g035480.3 | Mo-molybdopterin cofactor sulfurase | HC | chr...   271   6e-73
Medtr4g030930.1 | molybdenum cofactor sulfurase | HC | chr4:1052...   108   6e-24

>Medtr2g035480.1 | Mo-molybdopterin cofactor sulfurase | HC |
           chr2:15090768-15095115 | 20130731
          Length = 315

 Score =  484 bits (1246), Expect = e-137,   Method: Compositional matrix adjust.
 Identities = 229/303 (75%), Positives = 260/303 (85%), Gaps = 1/303 (0%)

Query: 30  KVAEIFIYPIKSCSGISLPSAPLRPTGLRWDREWMVVNEKGRGVTQRVEPKLAFVQVELP 89
           K++ IFIYPIKSC GISL  +PL P+GLRWDR+W+VVN KGR  TQRVEPKLA V+VELP
Sbjct: 13  KISAIFIYPIKSCRGISLSHSPLTPSGLRWDRQWVVVNSKGRACTQRVEPKLALVEVELP 72

Query: 90  NEAFLEDWEPSQDSFMVLKAPGMEPLKVCLNKQHEVANGLSVWEWSGSAWDEGAEASQWF 149
            EAF E WEP+ DSFMVLKAPGMEPLKV LNKQ+EVA+ ++VWEW+GSAWDEGAEA+QWF
Sbjct: 73  PEAFDEHWEPTTDSFMVLKAPGMEPLKVFLNKQYEVADDITVWEWTGSAWDEGAEAAQWF 132

Query: 150 SDYLGKPSQLVRFNTASEVRQVDPDYVKEHHSTFFTDGFPFLLISQESLDSLNEHLEEPI 209
           SDYLG P++LVRFNTASEVR+VDPDYV+    TFFTDG+PFLL+SQESLD+LN HLEEPI
Sbjct: 133 SDYLGNPTKLVRFNTASEVRKVDPDYVEGQQQTFFTDGYPFLLVSQESLDALNAHLEEPI 192

Query: 210 RINRFRPNILVEGCEPFSEDLWSEIKIGRFSFLGSKLCGRCKVTTINQETGIAGREPLET 269
            +NRFRPNILVEGCEP+SEDLW +IKI RFSF G KLC RCKV TINQET + G EP ET
Sbjct: 193 PMNRFRPNILVEGCEPYSEDLWRDIKISRFSFQGVKLCARCKVPTINQETAVGGSEPTET 252

Query: 270 LATFRSGKVIRPNG-KNKGAVYFGQNLVWNWNDSSAKGSGKVLKVGDPVYVLRKVSSPAE 328
           L   RSGKV+RPN  KNK  VYFGQNLVWNW+DSSAKG GKVLK+GDPV V++K+SSPAE
Sbjct: 253 LMKVRSGKVLRPNSDKNKNKVYFGQNLVWNWSDSSAKGDGKVLKLGDPVCVIKKLSSPAE 312

Query: 329 MPA 331
             A
Sbjct: 313 AAA 315


>Medtr2g035470.1 | Mo-molybdopterin cofactor sulfurase | HC |
           chr2:15096715-15100323 | 20130731
          Length = 319

 Score =  473 bits (1218), Expect = e-134,   Method: Compositional matrix adjust.
 Identities = 221/302 (73%), Positives = 255/302 (84%)

Query: 30  KVAEIFIYPIKSCSGISLPSAPLRPTGLRWDREWMVVNEKGRGVTQRVEPKLAFVQVELP 89
           KV+ IFIYP+KSC GISL  APL P+G RWDR+WMVVN KGR  TQRVEPKLA V+VE P
Sbjct: 18  KVSGIFIYPVKSCRGISLSQAPLTPSGFRWDRQWMVVNSKGRACTQRVEPKLALVEVEFP 77

Query: 90  NEAFLEDWEPSQDSFMVLKAPGMEPLKVCLNKQHEVANGLSVWEWSGSAWDEGAEASQWF 149
            EAF E WEP+ DSFMVLKAPGMEPLKVCLNKQ+EVA+ ++VWEW+GSAWDEGAEASQWF
Sbjct: 78  PEAFDEHWEPTTDSFMVLKAPGMEPLKVCLNKQYEVADDITVWEWTGSAWDEGAEASQWF 137

Query: 150 SDYLGKPSQLVRFNTASEVRQVDPDYVKEHHSTFFTDGFPFLLISQESLDSLNEHLEEPI 209
           SDYLG P++LVRFNTASEVR+VDPDYV+  + TFF+DG+PFL+ SQESLD+LNE LEEPI
Sbjct: 138 SDYLGNPTKLVRFNTASEVRKVDPDYVEGQYQTFFSDGYPFLIASQESLDALNELLEEPI 197

Query: 210 RINRFRPNILVEGCEPFSEDLWSEIKIGRFSFLGSKLCGRCKVTTINQETGIAGREPLET 269
            +NRFRPNILVEGCEP+SEDLW + KI  FSF G+KLC RCK+ TINQET   G EP ET
Sbjct: 198 LMNRFRPNILVEGCEPYSEDLWRDFKISSFSFQGAKLCYRCKIPTINQETAKVGPEPNET 257

Query: 270 LATFRSGKVIRPNGKNKGAVYFGQNLVWNWNDSSAKGSGKVLKVGDPVYVLRKVSSPAEM 329
           L  +RSG++IRPN KNK  VYFG ++VWNW +SSAK  GKVLK+GDPVYV++K SSPAE 
Sbjct: 258 LMKYRSGQIIRPNDKNKKRVYFGHDIVWNWMESSAKEDGKVLKLGDPVYVIKKFSSPAEA 317

Query: 330 PA 331
            A
Sbjct: 318 AA 319


>Medtr2g035460.1 | Mo-molybdopterin cofactor sulfurase | HC |
           chr2:15100868-15106361 | 20130731
          Length = 324

 Score =  473 bits (1217), Expect = e-133,   Method: Compositional matrix adjust.
 Identities = 222/303 (73%), Positives = 258/303 (85%), Gaps = 1/303 (0%)

Query: 30  KVAEIFIYPIKSCSGISLPSAPLRPTGLRWDREWMVVNEKGRGVTQRVEPKLAFVQVELP 89
           KV  +FIYP+KSC GI L +APL P+G RWDR+WMVVN KGR  T RVEP+ A + VELP
Sbjct: 22  KVTGLFIYPVKSCRGIPLSNAPLTPSGFRWDRQWMVVNSKGRACTIRVEPRFALIDVELP 81

Query: 90  NEAFLEDWEPSQDSFMVLKAPGMEPLKVCLNKQHEVANGLSVWEWSGSAWDEGAEASQWF 149
            EAFL+ WEP+ DSFMVLKAPGMEPLKVCLNKQ+EV + ++VWEW+GSAWDEGAEASQWF
Sbjct: 82  PEAFLQHWEPTTDSFMVLKAPGMEPLKVCLNKQYEVEDDVTVWEWTGSAWDEGAEASQWF 141

Query: 150 SDYLGKPSQLVRFNTASEVRQVDPDYVKEHHSTFFTDGFPFLLISQESLDSLNEHLEEPI 209
           SDYLG P++LVRFNTASEVR+VDPDYVK  H TFF+DG+PFLL+SQ+SLDSLNE LEEPI
Sbjct: 142 SDYLGYPAKLVRFNTASEVRKVDPDYVKGQHQTFFSDGYPFLLVSQDSLDSLNELLEEPI 201

Query: 210 RINRFRPNILVEGCEPFSEDLWSEIKIGRFSFLGSKLCGRCKVTTINQETGIAGREPLET 269
            +NRFRPNILVEGC P+SEDLW +IKI RFSF G+KLCGRCKV T++QET + G EP +T
Sbjct: 202 PMNRFRPNILVEGCGPYSEDLWRDIKISRFSFQGAKLCGRCKVPTVDQETAMYGDEPTKT 261

Query: 270 LATFRSGKVIRPNG-KNKGAVYFGQNLVWNWNDSSAKGSGKVLKVGDPVYVLRKVSSPAE 328
           L   RSG+V+RPN  KNK  VYFGQN+VWNWN+SSAKG  KVLK+GDPVYV++KVSSPAE
Sbjct: 262 LMKIRSGEVLRPNSDKNKKMVYFGQNVVWNWNESSAKGDEKVLKLGDPVYVIKKVSSPAE 321

Query: 329 MPA 331
             A
Sbjct: 322 AAA 324


>Medtr2g035460.2 | Mo-molybdopterin cofactor sulfurase | HC |
           chr2:15101357-15106361 | 20130731
          Length = 269

 Score =  423 bits (1088), Expect = e-118,   Method: Compositional matrix adjust.
 Identities = 200/269 (74%), Positives = 231/269 (85%), Gaps = 1/269 (0%)

Query: 64  MVVNEKGRGVTQRVEPKLAFVQVELPNEAFLEDWEPSQDSFMVLKAPGMEPLKVCLNKQH 123
           MVVN KGR  T RVEP+ A + VELP EAFL+ WEP+ DSFMVLKAPGMEPLKVCLNKQ+
Sbjct: 1   MVVNSKGRACTIRVEPRFALIDVELPPEAFLQHWEPTTDSFMVLKAPGMEPLKVCLNKQY 60

Query: 124 EVANGLSVWEWSGSAWDEGAEASQWFSDYLGKPSQLVRFNTASEVRQVDPDYVKEHHSTF 183
           EV + ++VWEW+GSAWDEGAEASQWFSDYLG P++LVRFNTASEVR+VDPDYVK  H TF
Sbjct: 61  EVEDDVTVWEWTGSAWDEGAEASQWFSDYLGYPAKLVRFNTASEVRKVDPDYVKGQHQTF 120

Query: 184 FTDGFPFLLISQESLDSLNEHLEEPIRINRFRPNILVEGCEPFSEDLWSEIKIGRFSFLG 243
           F+DG+PFLL+SQ+SLDSLNE LEEPI +NRFRPNILVEGC P+SEDLW +IKI RFSF G
Sbjct: 121 FSDGYPFLLVSQDSLDSLNELLEEPIPMNRFRPNILVEGCGPYSEDLWRDIKISRFSFQG 180

Query: 244 SKLCGRCKVTTINQETGIAGREPLETLATFRSGKVIRPNG-KNKGAVYFGQNLVWNWNDS 302
           +KLCGRCKV T++QET + G EP +TL   RSG+V+RPN  KNK  VYFGQN+VWNWN+S
Sbjct: 181 AKLCGRCKVPTVDQETAMYGDEPTKTLMKIRSGEVLRPNSDKNKKMVYFGQNVVWNWNES 240

Query: 303 SAKGSGKVLKVGDPVYVLRKVSSPAEMPA 331
           SAKG  KVLK+GDPVYV++KVSSPAE  A
Sbjct: 241 SAKGDEKVLKLGDPVYVIKKVSSPAEAAA 269


>Medtr2g035470.2 | Mo-molybdopterin cofactor sulfurase | HC |
           chr2:15098726-15100843 | 20130731
          Length = 226

 Score =  356 bits (914), Expect = 2e-98,   Method: Compositional matrix adjust.
 Identities = 165/226 (73%), Positives = 194/226 (85%)

Query: 106 VLKAPGMEPLKVCLNKQHEVANGLSVWEWSGSAWDEGAEASQWFSDYLGKPSQLVRFNTA 165
           +LKAPGMEPLKVCLNKQ+EVA+ ++VWEW+GSAWDEGAEASQWFSDYLG P++LVRFNTA
Sbjct: 1   MLKAPGMEPLKVCLNKQYEVADDITVWEWTGSAWDEGAEASQWFSDYLGNPTKLVRFNTA 60

Query: 166 SEVRQVDPDYVKEHHSTFFTDGFPFLLISQESLDSLNEHLEEPIRINRFRPNILVEGCEP 225
           SEVR+VDPDYV+  + TFF+DG+PFL+ SQESLD+LNE LEEPI +NRFRPNILVEGCEP
Sbjct: 61  SEVRKVDPDYVEGQYQTFFSDGYPFLIASQESLDALNELLEEPILMNRFRPNILVEGCEP 120

Query: 226 FSEDLWSEIKIGRFSFLGSKLCGRCKVTTINQETGIAGREPLETLATFRSGKVIRPNGKN 285
           +SEDLW + KI  FSF G+KLC RCK+ TINQET   G EP ETL  +RSG++IRPN KN
Sbjct: 121 YSEDLWRDFKISSFSFQGAKLCYRCKIPTINQETAKVGPEPNETLMKYRSGQIIRPNDKN 180

Query: 286 KGAVYFGQNLVWNWNDSSAKGSGKVLKVGDPVYVLRKVSSPAEMPA 331
           K  VYFG ++VWNW +SSAK  GKVLK+GDPVYV++K SSPAE  A
Sbjct: 181 KKRVYFGHDIVWNWMESSAKEDGKVLKLGDPVYVIKKFSSPAEAAA 226


>Medtr2g035480.2 | Mo-molybdopterin cofactor sulfurase | HC |
           chr2:15091556-15095067 | 20130731
          Length = 221

 Score =  356 bits (913), Expect = 2e-98,   Method: Compositional matrix adjust.
 Identities = 168/221 (76%), Positives = 191/221 (86%), Gaps = 1/221 (0%)

Query: 112 MEPLKVCLNKQHEVANGLSVWEWSGSAWDEGAEASQWFSDYLGKPSQLVRFNTASEVRQV 171
           MEPLKV LNKQ+EVA+ ++VWEW+GSAWDEGAEA+QWFSDYLG P++LVRFNTASEVR+V
Sbjct: 1   MEPLKVFLNKQYEVADDITVWEWTGSAWDEGAEAAQWFSDYLGNPTKLVRFNTASEVRKV 60

Query: 172 DPDYVKEHHSTFFTDGFPFLLISQESLDSLNEHLEEPIRINRFRPNILVEGCEPFSEDLW 231
           DPDYV+    TFFTDG+PFLL+SQESLD+LN HLEEPI +NRFRPNILVEGCEP+SEDLW
Sbjct: 61  DPDYVEGQQQTFFTDGYPFLLVSQESLDALNAHLEEPIPMNRFRPNILVEGCEPYSEDLW 120

Query: 232 SEIKIGRFSFLGSKLCGRCKVTTINQETGIAGREPLETLATFRSGKVIRPNG-KNKGAVY 290
            +IKI RFSF G KLC RCKV TINQET + G EP ETL   RSGKV+RPN  KNK  VY
Sbjct: 121 RDIKISRFSFQGVKLCARCKVPTINQETAVGGSEPTETLMKVRSGKVLRPNSDKNKNKVY 180

Query: 291 FGQNLVWNWNDSSAKGSGKVLKVGDPVYVLRKVSSPAEMPA 331
           FGQNLVWNW+DSSAKG GKVLK+GDPV V++K+SSPAE  A
Sbjct: 181 FGQNLVWNWSDSSAKGDGKVLKLGDPVCVIKKLSSPAEAAA 221


>Medtr2g035480.3 | Mo-molybdopterin cofactor sulfurase | HC |
           chr2:15090768-15095115 | 20130731
          Length = 193

 Score =  271 bits (693), Expect = 6e-73,   Method: Compositional matrix adjust.
 Identities = 128/176 (72%), Positives = 146/176 (82%), Gaps = 1/176 (0%)

Query: 157 SQLVRFNTASEVRQVDPDYVKEHHSTFFTDGFPFLLISQESLDSLNEHLEEPIRINRFRP 216
           S+++  N ASEVR+VDPDYV+    TFFTDG+PFLL+SQESLD+LN HLEEPI +NRFRP
Sbjct: 18  SRVIYLNAASEVRKVDPDYVEGQQQTFFTDGYPFLLVSQESLDALNAHLEEPIPMNRFRP 77

Query: 217 NILVEGCEPFSEDLWSEIKIGRFSFLGSKLCGRCKVTTINQETGIAGREPLETLATFRSG 276
           NILVEGCEP+SEDLW +IKI RFSF G KLC RCKV TINQET + G EP ETL   RSG
Sbjct: 78  NILVEGCEPYSEDLWRDIKISRFSFQGVKLCARCKVPTINQETAVGGSEPTETLMKVRSG 137

Query: 277 KVIRPNG-KNKGAVYFGQNLVWNWNDSSAKGSGKVLKVGDPVYVLRKVSSPAEMPA 331
           KV+RPN  KNK  VYFGQNLVWNW+DSSAKG GKVLK+GDPV V++K+SSPAE  A
Sbjct: 138 KVLRPNSDKNKNKVYFGQNLVWNWSDSSAKGDGKVLKLGDPVCVIKKLSSPAEAAA 193


>Medtr4g030930.1 | molybdenum cofactor sulfurase | HC |
           chr4:10524602-10538101 | 20130731
          Length = 742

 Score =  108 bits (271), Expect = 6e-24,   Method: Compositional matrix adjust.
 Identities = 91/308 (29%), Positives = 141/308 (45%), Gaps = 41/308 (13%)

Query: 33  EIFIYPIKSCSGISLPSAPLRPTG-LRWDREWMVVNEKGRGVTQRVEPKLAFVQVELPNE 91
           ++ +YPIKSC G S  S PL   G L+ DREW++ +  G  +T +  P++  +       
Sbjct: 452 QLKVYPIKSCGGFSASSWPLSNNGSLKHDREWILKSLSGEILTLKRVPEMGLIS------ 505

Query: 92  AFLEDWEPSQDSFMVLKAPGMEPLKVCLNKQHEVANGLSVWEWSGS---AWDEGAEASQW 148
           +F++    SQ    V      E L++ L       + +   E  G     +    E + W
Sbjct: 506 SFID---LSQGMLFVESPHCKERLQIRLQLDF-YDSAIQDIELQGQRYKVYSYDNETNSW 561

Query: 149 FSDYLGKPSQLVRFNTASEV----RQVDPDYVKEHHSTF-FTDGFPFLLISQESLDSLNE 203
           FS  + +P  L+R++ +S      R  D    K+ +S   F +   FLL+S+ES+  LN+
Sbjct: 562 FSKAIERPCTLLRYSGSSHDFVLDRTKDIVTCKDTNSAVSFANEGQFLLVSEESVSDLNK 621

Query: 204 HL-----------EEPIRINRFRPNILVEGCEPFSEDLWSEIKIGRFSFLGSKLCGRCKV 252
            L           E  I  NRFRPN++V G  P+ ED WS+I+IG   F     C RC+V
Sbjct: 622 RLCSDVQMDMCETEIEINTNRFRPNLVVSGGRPYDEDGWSDIRIGNKYFRSLGGCNRCQV 681

Query: 253 TTINQETGIA--GREPLETLATFRSGKVIRPNGKNKGAVYFGQNLVWNWNDSSAKGSGKV 310
             +    G     +EPL TLA++R         + KG + FG  L +   +   +     
Sbjct: 682 INLTLNAGQVQKSKEPLATLASYR---------RVKGRILFGILLKYVSVNGEQQQGDSW 732

Query: 311 LKVGDPVY 318
           L VG  V+
Sbjct: 733 LHVGQEVH 740