Miyakogusa Predicted Gene

Lj6g3v0657890.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj6g3v0657890.2 tr|I1LN46|I1LN46_SOYBN DNA topoisomerase
OS=Glycine max GN=Gma.49661 PE=3 SV=1,90.88,0,PRTPISMRASEI,DNA
topoisomerase, type IA; TOPOISOMERASE_I_PROK,DNA topoisomerase, type
IA, active sit,CUFF.58128.2
         (669 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr3g084800.1 | DNA topoisomerase I | HC | chr3:38297430-38308...  1194   0.0  
Medtr3g084800.2 | DNA topoisomerase I | HC | chr3:38297420-38308...  1192   0.0  
Medtr3g096640.1 | DNA topoisomerase | HC | chr3:44222682-4420848...   231   1e-60
Medtr5g061930.2 | DNA topoisomerase | HC | chr5:25738278-2573623...   146   8e-35
Medtr5g061930.1 | DNA topoisomerase | HC | chr5:25738278-2573529...   146   8e-35
Medtr5g061940.1 | DNA topoisomerase I | HC | chr5:25738373-25739...    64   4e-10

>Medtr3g084800.1 | DNA topoisomerase I | HC | chr3:38297430-38308919
           | 20130731
          Length = 860

 Score = 1194 bits (3090), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 571/664 (85%), Positives = 601/664 (90%), Gaps = 2/664 (0%)

Query: 7   LIAVYFSYGPCQTPTLGFCVQRYLQINTFKPEKFWSVNPYIVHSGYEIQLEWQRSKLFDI 66
           L A   SYGPCQTPTLGFCVQRYLQINTFKPEKFWS+ PYI+ SGYEI LEWQRSKLFDI
Sbjct: 198 LDARVISYGPCQTPTLGFCVQRYLQINTFKPEKFWSLQPYIIQSGYEILLEWQRSKLFDI 257

Query: 67  NVATMFQKLVAEDGLLEVTEISEKQETKSRPVGLNTVNLLKVASSALGFGPHMAMQLAER 126
           NVA MFQKLVA+DG++EVT ISEK ETK RPVGLNTVNLLKVASSALGFGP MAMQLAER
Sbjct: 258 NVAMMFQKLVADDGIVEVTNISEKPETKGRPVGLNTVNLLKVASSALGFGPQMAMQLAER 317

Query: 127 LYTQGFISYPRTESTAYPPSFDFRGALSAQANNPTWGNYVEGLLTNGYLKPRLGTDVGDH 186
           LYTQGFISYPRTESTAYPPSFDFR ALSAQ NNPTWGNYVEGLL +GY KPR GTDVGDH
Sbjct: 318 LYTQGFISYPRTESTAYPPSFDFRSALSAQRNNPTWGNYVEGLLASGYQKPRSGTDVGDH 377

Query: 187 PPITPMRPAAEDMLGNDAWKLYQYICQHFIGTVSPDCKYARRKVEFSIGGESFHCTGQHV 246
           PPITPM+ A+EDMLGNDAWKLYQYICQ+FIGTVSPDCKY R+KVEF +GGESFHCTGQHV
Sbjct: 378 PPITPMKSASEDMLGNDAWKLYQYICQYFIGTVSPDCKYIRKKVEFQVGGESFHCTGQHV 437

Query: 247 ITKGFTAIMPWLAINDKNIPSFIKGQKIEVSKVELYEGTTTPPDYLTESELISLMEKNGI 306
           ITKGFTAIMPWLAINDK++PSF +GQKI++SKVELYEG+TTPPD+LTESELISLMEKNGI
Sbjct: 438 ITKGFTAIMPWLAINDKSLPSFEEGQKIKLSKVELYEGSTTPPDFLTESELISLMEKNGI 497

Query: 307 GTDASIPVHINNICERNYVQVQAGRKLVPTTLGITLVRGYQSIDPDLCLPDIRSFIEQQI 366
           GTDASIPVHINNICERNYVQVQAGRKL PTTLGITLVRGYQSIDPDLCLPDIRSFIEQQI
Sbjct: 498 GTDASIPVHINNICERNYVQVQAGRKLSPTTLGITLVRGYQSIDPDLCLPDIRSFIEQQI 557

Query: 367 TLIAKGQIDHHRVVQHVIQQFKQKFCYFVKKIEAMDALFEAQFSTLVDSGRILSKCGKCL 426
           TLIAKGQ+DHHRVVQHVI+QFKQKFCYFVKKIEAMDALFEAQFS LVDSGRI+SKCGKCL
Sbjct: 558 TLIAKGQVDHHRVVQHVIEQFKQKFCYFVKKIEAMDALFEAQFSALVDSGRIMSKCGKCL 617

Query: 427 RYMKYISAQPSRLYCGICEEVYYLPQKGTIKLYKELSCPLDNFELLLCSM-GPDGKSFPL 485
           RYMKYIS QPSRLYCG CEEVY LPQKGTIKLYKEL+CPLDNFELL+CSM GP+GKSFPL
Sbjct: 618 RYMKYISVQPSRLYCGTCEEVYDLPQKGTIKLYKELTCPLDNFELLICSMPGPEGKSFPL 677

Query: 486 CPYCYSNPPFEGIEALINTAKTSTSGKIGKGAGMPCILCPHPTCPNSLVSQGVCACPECS 545
           CPYCYSNPPFEGI+ LIN+AKT+TS K GKGAGMPC LCPHPTCPNSLVSQGVCACPECS
Sbjct: 678 CPYCYSNPPFEGIDTLINSAKTTTSSKTGKGAGMPCNLCPHPTCPNSLVSQGVCACPECS 737

Query: 546 GTLVLDPVSAPKWRLCCNMCNCLVFLAQGAHRISTTKERCPECDSSIIEVDFNKKATPLE 605
           GTLVLDPVSAPKWRLCCNMCNCLVFLAQGAHRISTTKERCPECDSSI+EVDFNKK TPL 
Sbjct: 738 GTLVLDPVSAPKWRLCCNMCNCLVFLAQGAHRISTTKERCPECDSSILEVDFNKKTTPLA 797

Query: 606 DGSTLHRGCILCDELLHSLVEMKHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMVDPKMS 665
           DGSTLHRGCILCD LLHSLVEMK+                             M+DPKMS
Sbjct: 798 DGSTLHRGCILCDVLLHSLVEMKY-GRGFKSSRGRGRGGRTQRYRGRGRGNAKMMDPKMS 856

Query: 666 FRDF 669
           FRDF
Sbjct: 857 FRDF 860


>Medtr3g084800.2 | DNA topoisomerase I | HC | chr3:38297420-38308924
           | 20130731
          Length = 800

 Score = 1192 bits (3083), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 571/664 (85%), Positives = 601/664 (90%), Gaps = 2/664 (0%)

Query: 7   LIAVYFSYGPCQTPTLGFCVQRYLQINTFKPEKFWSVNPYIVHSGYEIQLEWQRSKLFDI 66
           L A   SYGPCQTPTLGFCVQRYLQINTFKPEKFWS+ PYI+ SGYEI LEWQRSKLFDI
Sbjct: 138 LDARVISYGPCQTPTLGFCVQRYLQINTFKPEKFWSLQPYIIQSGYEILLEWQRSKLFDI 197

Query: 67  NVATMFQKLVAEDGLLEVTEISEKQETKSRPVGLNTVNLLKVASSALGFGPHMAMQLAER 126
           NVA MFQKLVA+DG++EVT ISEK ETK RPVGLNTVNLLKVASSALGFGP MAMQLAER
Sbjct: 198 NVAMMFQKLVADDGIVEVTNISEKPETKGRPVGLNTVNLLKVASSALGFGPQMAMQLAER 257

Query: 127 LYTQGFISYPRTESTAYPPSFDFRGALSAQANNPTWGNYVEGLLTNGYLKPRLGTDVGDH 186
           LYTQGFISYPRTESTAYPPSFDFR ALSAQ NNPTWGNYVEGLL +GY KPR GTDVGDH
Sbjct: 258 LYTQGFISYPRTESTAYPPSFDFRSALSAQRNNPTWGNYVEGLLASGYQKPRSGTDVGDH 317

Query: 187 PPITPMRPAAEDMLGNDAWKLYQYICQHFIGTVSPDCKYARRKVEFSIGGESFHCTGQHV 246
           PPITPM+ A+EDMLGNDAWKLYQYICQ+FIGTVSPDCKY R+KVEF +GGESFHCTGQHV
Sbjct: 318 PPITPMKSASEDMLGNDAWKLYQYICQYFIGTVSPDCKYIRKKVEFQVGGESFHCTGQHV 377

Query: 247 ITKGFTAIMPWLAINDKNIPSFIKGQKIEVSKVELYEGTTTPPDYLTESELISLMEKNGI 306
           ITKGFTAIMPWLAINDK++PSF +GQKI++SKVELYEG+TTPPD+LTESELISLMEKNGI
Sbjct: 378 ITKGFTAIMPWLAINDKSLPSFEEGQKIKLSKVELYEGSTTPPDFLTESELISLMEKNGI 437

Query: 307 GTDASIPVHINNICERNYVQVQAGRKLVPTTLGITLVRGYQSIDPDLCLPDIRSFIEQQI 366
           GTDASIPVHINNICERNYVQVQAGRKL PTTLGITLVRGYQSIDPDLCLPDIRSFIEQQI
Sbjct: 438 GTDASIPVHINNICERNYVQVQAGRKLSPTTLGITLVRGYQSIDPDLCLPDIRSFIEQQI 497

Query: 367 TLIAKGQIDHHRVVQHVIQQFKQKFCYFVKKIEAMDALFEAQFSTLVDSGRILSKCGKCL 426
           TLIAKGQ+DHHRVVQHVI+QFKQKFCYFVKKIEAMDALFEAQFS LVDSGRI+SKCGKCL
Sbjct: 498 TLIAKGQVDHHRVVQHVIEQFKQKFCYFVKKIEAMDALFEAQFSALVDSGRIMSKCGKCL 557

Query: 427 RYMKYISAQPSRLYCGICEEVYYLPQKGTIKLYKELSCPLDNFELLLCSM-GPDGKSFPL 485
           RYMKYIS QPSRLYCG CEEVY LPQKGTIKLYKEL+CPLDNFELL+CSM GP+GKSFPL
Sbjct: 558 RYMKYISVQPSRLYCGTCEEVYDLPQKGTIKLYKELTCPLDNFELLICSMPGPEGKSFPL 617

Query: 486 CPYCYSNPPFEGIEALINTAKTSTSGKIGKGAGMPCILCPHPTCPNSLVSQGVCACPECS 545
           CPYCYSNPPFEGI+ LIN+AKT+TS K GKGAGMPC LCPHPTCPNSLVSQGVCACPECS
Sbjct: 618 CPYCYSNPPFEGIDTLINSAKTTTSSKTGKGAGMPCNLCPHPTCPNSLVSQGVCACPECS 677

Query: 546 GTLVLDPVSAPKWRLCCNMCNCLVFLAQGAHRISTTKERCPECDSSIIEVDFNKKATPLE 605
           GTLVLDPVSAPKWRLCCNMCNCLVFLAQGAHRISTTKERCPECDSSI+EVDFNKK TPL 
Sbjct: 678 GTLVLDPVSAPKWRLCCNMCNCLVFLAQGAHRISTTKERCPECDSSILEVDFNKKTTPLA 737

Query: 606 DGSTLHRGCILCDELLHSLVEMKHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMVDPKMS 665
           DGSTLHRGCILCD LLHSLVEMK+                             M+DPKMS
Sbjct: 738 DGSTLHRGCILCDVLLHSLVEMKY-GRGFKSSRGRGRGGRTQRYRGRGRGNAKMMDPKMS 796

Query: 666 FRDF 669
           FRDF
Sbjct: 797 FRDF 800


>Medtr3g096640.1 | DNA topoisomerase | HC | chr3:44222682-44208485 |
           20130731
          Length = 956

 Score =  231 bits (590), Expect = 1e-60,   Method: Compositional matrix adjust.
 Identities = 142/429 (33%), Positives = 211/429 (49%), Gaps = 17/429 (3%)

Query: 12  FSYGPCQTPTLGFCVQRYLQINTFKPEKFWSVNPYIVHSGYEIQLEWQRSKLFDINVATM 71
            SYGPCQ PTLGF V+RY +I   +PE+FW +              W+R  LFD   A +
Sbjct: 216 LSYGPCQFPTLGFVVERYWEIQAHEPEEFWLIKCSHRSDEGVANFSWERGHLFDYTCAVI 275

Query: 72  FQKLVAEDGLLEVTEISEKQETKSRPVGLNTVNLLKVASSALGFGPHMAMQLAERLYTQG 131
             ++  E+    VT+++ K++ K  P  L+T+ L K AS          M++AE LY  G
Sbjct: 276 IYEMCIEEPTATVTKVTPKEKPKYPPHPLSTIELEKRASRYFRMSSERTMKVAEELYQGG 335

Query: 132 FISYPRTESTAYPPSFDFRGALSAQANNPTWGNYVEGLLTNG---YLKPRLGT-DVGDHP 187
           FISYPRTE+  +    D    +  Q  +P WG Y + LL  G   +  PR G  D   HP
Sbjct: 336 FISYPRTETDNFSSRTDLHAIVQEQQGHPEWGIYAQRLLDPGAGLWRNPRGGGHDDQAHP 395

Query: 188 PITPMR-PAAEDMLGNDAWKLYQYICQHFIGTVSPDCKYARRKVEFSIGGESFHCTGQHV 246
           PI P +  A E     D  KLY+ + +HF+  VS     A   VE  I GE F   G+ +
Sbjct: 396 PIHPTKFSAGESGWSQDHRKLYELVVRHFLACVSKPALGAETTVEIDIAGERFSACGRVI 455

Query: 247 ITKGFTAIMPWLAINDKNIPSFIKGQKIEVSKVELYEGTTTPPDYLTESELISLMEKNGI 306
           + + +  I  +   +   IP++  G++   + + L  G T PP  L+E++L+S M+K  I
Sbjct: 456 LERNYLDIYIYDRWDGSMIPNYAIGEQFIPTTLTLDPGKTNPPPLLSEADLLSRMDKECI 515

Query: 307 GTDASIPVHINNICERNYVQVQAGRKLVPTTLGITLVRGYQSIDPDLCLPDIRSFIEQQI 366
           GTDA++  HI  + +R Y    A     PT LG  LV GY  +   L  PD+R+ +E+ +
Sbjct: 516 GTDATMHDHIKKLLDRFYATKDANMCFTPTNLGEALVMGYDDMGYKLWKPDLRAVMERDM 575

Query: 367 TLIAKGQIDHHRVVQHVIQQFKQKFCYF------VKKIEAMDALFEAQFSTLVD----SG 416
             +++G      V+   +QQ K   C+       VK +EAM   FE       D    +G
Sbjct: 576 KSVSEGNKSKAEVLDTSLQQMKT--CFLDARLNKVKLLEAMAIFFERLNRVGSDEQNGTG 633

Query: 417 RILSKCGKC 425
            ++ +C  C
Sbjct: 634 VVVRRCRLC 642


>Medtr5g061930.2 | DNA topoisomerase | HC | chr5:25738278-25736234 |
           20130731
          Length = 438

 Score =  146 bits (368), Expect = 8e-35,   Method: Compositional matrix adjust.
 Identities = 109/379 (28%), Positives = 176/379 (46%), Gaps = 25/379 (6%)

Query: 12  FSYGPCQTPTLGFCVQRYLQINTFKPEKFWSVNPYIVHSGYEIQLEWQRSKLFDINVATM 71
            SYG CQ+  LG  V+R+ Q+   K +K+   N      GY  ++ +     +D      
Sbjct: 55  LSYGLCQSALLGIVVERFNQVAKPKAQKY---NISFNDRGYGPKVTFTSPVWYDAKSKAS 111

Query: 72  FQKLVAEDGLLEVTEISEKQETK--SRPVGLNTVNLLKVASSALGFGPHMAMQLAERLYT 129
            +     D    + E+  +   K  S PV L T+ L K A+      P   M  AE LYT
Sbjct: 112 KKLQSCSDEKATIVEVFVEITNKFHSPPVPLTTMELEKFAAKYFKMTPKQTMDTAEDLYT 171

Query: 130 QGFISYPRTESTAYP-PSFDFRGALSAQANNPTWGNYVEGLLTNG---YLKPRL--GTDV 183
           Q FISYPRTE+  +   + + R  +S Q  +  WG+Y   LL +    +  PR    TD 
Sbjct: 172 QRFISYPRTETDGFSMNTKELRSIVSKQTKHEKWGSYASDLLDSNKGLWRDPRACKHTD- 230

Query: 184 GDHPPITPMRPAAEDMLGNDAWK-----LYQYICQHFIGTVSPDCKYARRKVEFSIGGES 238
             HPPI P++ +     G   WK     LY+ I +HF+  +S D    +  VE  + G+ 
Sbjct: 231 HSHPPIHPIKFSNRKESG---WKDHQCVLYELIVRHFLACLSEDALVQKTTVEVDLAGQQ 287

Query: 239 FHCTGQHVITKGFTAIMPW--LAINDKNIPSFIKGQKIEVSKVELYEGTTTPPDYLTESE 296
           F      +   G+  +  +  L +     P +  G ++    + +   T + P  L++ +
Sbjct: 288 FFAMTMTLYKLGYRKMYHYESLFLGFSKFPPYTAGGELTPMDLIVKGSTYSSPQNLSDED 347

Query: 297 LISLMEKNGIGTDASIPVHINNICERNYVQVQ---AGRKLVPTTLGITLVRGYQSIDPDL 353
           L+S M++  IGTD++  + IN +  R YV+ +      +  PT LG+ L  GY  +   L
Sbjct: 348 LLSRMQQERIGTDSTWHLLINTVIGRKYVERKFDGNTWRYEPTKLGLALFNGYDQMKLPL 407

Query: 354 CLPDIRSFIEQQITLIAKG 372
             P++R+ +E+QIT+I  G
Sbjct: 408 WKPEMRAKMEKQITMIKNG 426


>Medtr5g061930.1 | DNA topoisomerase | HC | chr5:25738278-25735295 |
           20130731
          Length = 438

 Score =  146 bits (368), Expect = 8e-35,   Method: Compositional matrix adjust.
 Identities = 109/379 (28%), Positives = 176/379 (46%), Gaps = 25/379 (6%)

Query: 12  FSYGPCQTPTLGFCVQRYLQINTFKPEKFWSVNPYIVHSGYEIQLEWQRSKLFDINVATM 71
            SYG CQ+  LG  V+R+ Q+   K +K+   N      GY  ++ +     +D      
Sbjct: 55  LSYGLCQSALLGIVVERFNQVAKPKAQKY---NISFNDRGYGPKVTFTSPVWYDAKSKAS 111

Query: 72  FQKLVAEDGLLEVTEISEKQETK--SRPVGLNTVNLLKVASSALGFGPHMAMQLAERLYT 129
            +     D    + E+  +   K  S PV L T+ L K A+      P   M  AE LYT
Sbjct: 112 KKLQSCSDEKATIVEVFVEITNKFHSPPVPLTTMELEKFAAKYFKMTPKQTMDTAEDLYT 171

Query: 130 QGFISYPRTESTAYP-PSFDFRGALSAQANNPTWGNYVEGLLTNG---YLKPRL--GTDV 183
           Q FISYPRTE+  +   + + R  +S Q  +  WG+Y   LL +    +  PR    TD 
Sbjct: 172 QRFISYPRTETDGFSMNTKELRSIVSKQTKHEKWGSYASDLLDSNKGLWRDPRACKHTD- 230

Query: 184 GDHPPITPMRPAAEDMLGNDAWK-----LYQYICQHFIGTVSPDCKYARRKVEFSIGGES 238
             HPPI P++ +     G   WK     LY+ I +HF+  +S D    +  VE  + G+ 
Sbjct: 231 HSHPPIHPIKFSNRKESG---WKDHQCVLYELIVRHFLACLSEDALVQKTTVEVDLAGQQ 287

Query: 239 FHCTGQHVITKGFTAIMPW--LAINDKNIPSFIKGQKIEVSKVELYEGTTTPPDYLTESE 296
           F      +   G+  +  +  L +     P +  G ++    + +   T + P  L++ +
Sbjct: 288 FFAMTMTLYKLGYRKMYHYESLFLGFSKFPPYTAGGELTPMDLIVKGSTYSSPQNLSDED 347

Query: 297 LISLMEKNGIGTDASIPVHINNICERNYVQVQ---AGRKLVPTTLGITLVRGYQSIDPDL 353
           L+S M++  IGTD++  + IN +  R YV+ +      +  PT LG+ L  GY  +   L
Sbjct: 348 LLSRMQQERIGTDSTWHLLINTVIGRKYVERKFDGNTWRYEPTKLGLALFNGYDQMKLPL 407

Query: 354 CLPDIRSFIEQQITLIAKG 372
             P++R+ +E+QIT+I  G
Sbjct: 408 WKPEMRAKMEKQITMIKNG 426


>Medtr5g061940.1 | DNA topoisomerase I | HC | chr5:25738373-25739284
           | 20130731
          Length = 303

 Score = 64.3 bits (155), Expect = 4e-10,   Method: Compositional matrix adjust.
 Identities = 47/137 (34%), Positives = 68/137 (49%), Gaps = 8/137 (5%)

Query: 12  FSYGPCQTPTLGFCVQRYLQINTFKPEKFWSVNPYIVHSGYEIQLEWQRSKLFDIN--VA 69
            SYG CQ+  LG  V+R+ Q+   K +K+   N      GY  ++ +     +D     +
Sbjct: 167 LSYGLCQSALLGIVVERFNQVAKPKAQKY---NISFNDRGYGPKVTFTSPVWYDAKSKAS 223

Query: 70  TMFQKLVAEDG-LLEV-TEISEK-QETKSRPVGLNTVNLLKVASSALGFGPHMAMQLAER 126
              Q    E   ++EV  EI+ K  +  S PV L T+ L K+A+      P   M  AE 
Sbjct: 224 KKLQSCSDEKATIVEVFVEITNKFNKFHSPPVPLTTMELEKLAAKYFKMTPKQTMNTAED 283

Query: 127 LYTQGFISYPRTESTAY 143
           LYTQGFI+YPRTE+  +
Sbjct: 284 LYTQGFINYPRTETDGF 300