Miyakogusa Predicted Gene

Lj5g3v2240910.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v2240910.1 Non Characterized Hit- tr|D7TXX5|D7TXX5_VITVI
Putative uncharacterized protein OS=Vitis vinifera
GN=,92.81,0,Radical_SAM,Radical SAM; Elongator protein 3, MiaB family,
Radical SA,Elongator protein 3/MiaB/NifB;,CUFF.57005.1
         (340 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g050432.1 | lipoyl synthase | HC | chr1:19390953-19395413 ...   586   e-167
Medtr2g090140.1 | lipoyl synthase | HC | chr2:38226099-38230633 ...   353   1e-97
Medtr1g069485.1 | lipoate synthase, putative | LC | chr1:3009662...    55   1e-07

>Medtr1g050432.1 | lipoyl synthase | HC | chr1:19390953-19395413 |
           20130731
          Length = 389

 Score =  586 bits (1510), Expect = e-167,   Method: Compositional matrix adjust.
 Identities = 291/371 (78%), Positives = 306/371 (82%), Gaps = 32/371 (8%)

Query: 1   MIQQSLFNPPSIPFSTT----TTKRSRF-----------------------HHPPSASAK 33
           M  QSL  P S  FS+     TTK S F                         P S SA 
Sbjct: 1   MFHQSLLQPCSFSFSSNPFHKTTKPSSFTFTLRCQANDPSLSHSEVVVSPQQQPLSVSA- 59

Query: 34  LTTTTESEQQKS----GLGPYTGRDPNVKKPEWLRQKAPQGERFQQIKDSLSHLKLNTVC 89
           ++   ESE +      GLGP+TGRDPNVKKP WLRQKAPQGERF QIK+S+S LKLNTVC
Sbjct: 60  MSKLMESENKNGPYPGGLGPFTGRDPNVKKPAWLRQKAPQGERFSQIKESISQLKLNTVC 119

Query: 90  EEAQCPNIGECWNGGGDGIATATIMLLGDTCTRGCRFCAVKTSRNPAPPDPMEPENTANA 149
           EEAQCPNIGECWNGG DGIATATIM+LGDTCTRGCRFCAVKTS NPAPPDPMEPENTA A
Sbjct: 120 EEAQCPNIGECWNGGDDGIATATIMVLGDTCTRGCRFCAVKTSINPAPPDPMEPENTAKA 179

Query: 150 IASWDVDYIVLTSVDRDDIPDGGSGHFAQTVKAMKNLKPEIMVECLTSDFRGDLTAVETL 209
           IASW VDYIV+TSVDRDDIPDGGSGHFAQTVKAMKN+KPEIMVECLTSDFRGDL AVETL
Sbjct: 180 IASWGVDYIVITSVDRDDIPDGGSGHFAQTVKAMKNIKPEIMVECLTSDFRGDLKAVETL 239

Query: 210 VHSGLDVFAHNVETVKRLQRIVRDPRAGYEQSLSVLKHAKHSKEGMITKTSIMLGLGESD 269
           VHSGLDVFAHN+ETVKRLQRIVRDPRAGYEQSL+VL HAK SKEGMITK+SIMLGLGESD
Sbjct: 240 VHSGLDVFAHNIETVKRLQRIVRDPRAGYEQSLAVLNHAKQSKEGMITKSSIMLGLGESD 299

Query: 270 DELKEVMADLRAIDVDILTLGQYLQPTPLHLTVKEYVTPEKFAFWKEYGESIGFRYVASG 329
           DE+KE M DLRAI+VDILTLGQYLQPTPLHLTVKEYVTPEKF FWKEYGESIGFRYVASG
Sbjct: 300 DEVKETMMDLRAINVDILTLGQYLQPTPLHLTVKEYVTPEKFDFWKEYGESIGFRYVASG 359

Query: 330 PLVRSSYRAGE 340
           PLVRSSYRAGE
Sbjct: 360 PLVRSSYRAGE 370


>Medtr2g090140.1 | lipoyl synthase | HC | chr2:38226099-38230633 |
           20130731
          Length = 378

 Score =  353 bits (907), Expect = 1e-97,   Method: Compositional matrix adjust.
 Identities = 172/291 (59%), Positives = 221/291 (75%), Gaps = 2/291 (0%)

Query: 51  TGRDPNVKKPEWLRQKAPQGERFQQIKDSLSHLKLNTVCEEAQCPNIGECWNGGGDGIAT 110
           T ++P + KP+W+++  P G ++ QIK  L  LKL+TVCEEA+CPN+GECW+GG  G AT
Sbjct: 72  TKKNP-LPKPKWMKESIPGGWKYVQIKKKLRELKLHTVCEEAKCPNMGECWSGGETGTAT 130

Query: 111 ATIMLLGDTCTRGCRFCAVKTSRNPAPPDPMEPENTANAIASWDVDYIVLTSVDRDDIPD 170
           ATIM+LGDTCTRGCRFC VKTSR P PPDP EP N A AIASW +DY+V+TSVDRDD+PD
Sbjct: 131 ATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVITSVDRDDLPD 190

Query: 171 GGSGHFAQTVKAMKNLKPEIMVECLTSDFRGDLTAVETLVHSGLDVFAHNVETVKRLQRI 230
            GS HF +TV+ +K LKP I++E L  DFRG+   VE +  SGLDVFAHN+ETV+ LQ  
Sbjct: 191 QGSSHFTETVQKLKILKPSILIEALVPDFRGNAECVEKVSKSGLDVFAHNIETVEELQSA 250

Query: 231 VRDPRAGYEQSLSVLKHAK-HSKEGMITKTSIMLGLGESDDELKEVMADLRAIDVDILTL 289
           VRD RA + QSL VL+ AK ++  G +TKTSIMLG GE+ D++ + M  +RA  VD++T 
Sbjct: 251 VRDHRANFNQSLDVLRMAKDYAPAGTLTKTSIMLGCGETPDQIVKTMEKVRAAGVDVMTF 310

Query: 290 GQYLQPTPLHLTVKEYVTPEKFAFWKEYGESIGFRYVASGPLVRSSYRAGE 340
           GQY++P+  H+ V EY+TPE F  ++  G  +GFRYVASGP+VRSSY+AGE
Sbjct: 311 GQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361


>Medtr1g069485.1 | lipoate synthase, putative | LC |
           chr1:30096621-30095771 | 20130731
          Length = 80

 Score = 54.7 bits (130), Expect = 1e-07,   Method: Composition-based stats.
 Identities = 36/80 (45%), Positives = 41/80 (51%), Gaps = 16/80 (20%)

Query: 255 MITKTSIMLGLGESDDELKEVMADLRAIDVDILTLGQYLQPTPLHLTVKEYVTPEKFAFW 314
           MITK+SIMLGLGESDDE+KE   D+               P  +   VKEYV+       
Sbjct: 1   MITKSSIMLGLGESDDEVKETTMDI---------------PKSV-FAVKEYVSLTNLLSG 44

Query: 315 KEYGESIGFRYVASGPLVRS 334
           K  G    F YVASGPL  S
Sbjct: 45  KNMGSLSVFCYVASGPLFGS 64