Miyakogusa Predicted Gene

Lj5g3v2220290.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v2220290.2 tr|A9S5X8|A9S5X8_PHYPA RNA polymerase II
transcription elongation factor SPT5 OS=Physcomitrella
pate,30.8,4e-17,KOW (Kyprides, Ouzounis, Woese) motif.,KOW;
Translation proteins SH3-like domain,Translation protein,CUFF.56974.2
         (788 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g111560.1 | transcription elongation factor Spt5-like prot...   790   0.0  
Medtr3g089910.1 | transcription elongation factor SPT5-like prot...   200   5e-51
Medtr1g023975.1 | global transcription factor group protein | HC...   129   1e-29
Medtr5g062110.1 | hypothetical protein | LC | chr5:25797247-2579...   114   4e-25
Medtr7g050470.1 | transcription elongation factor SPT5-like prot...    78   4e-14

>Medtr1g111560.1 | transcription elongation factor Spt5-like
           protein, putative | HC | chr1:50409508-50417420 |
           20130731
          Length = 1326

 Score =  790 bits (2040), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 424/664 (63%), Positives = 489/664 (73%), Gaps = 28/664 (4%)

Query: 4   KGKQVAGKDSAGKRKANFXXXXXXXXXXXXXXXVVQFFEEAAAVXXXXXXXXXXXXXXXX 63
           KGK  AGK S+GKRK NF               VVQFFE+ AA                 
Sbjct: 11  KGKAPAGKGSSGKRKNNFDDDKTGKRKNRG---VVQFFEDEAA--DVDSDDSEFSDFSDE 65

Query: 64  XXXXEYETLSTMLNDTNASAKSQSRPAFVPKEEVVDEE-FDRFLEERYAGGSKFSHFA-- 120
               E+ETLST  N+   SAK  S    VPK+E+VDEE FDR +EER    S+F  FA  
Sbjct: 66  SYEDEFETLSTTRNE---SAKGSSSVPRVPKQELVDEEEFDRLMEERV--NSRFFRFAED 120

Query: 121 -DEYDDKPM-DPSSMHPGAKEVTPAIWKVKCTVGRERLSAFCLMQKFADMKKLGTNLKLI 178
            DE++DKPM D SS+H   KE  P IW+VKCTVGRERLSA CLMQKF  +K L T L+LI
Sbjct: 121 GDEFEDKPMEDNSSLHHALKESIPTIWRVKCTVGRERLSAICLMQKFVHLKGLDTKLQLI 180

Query: 179 SAFAVDHMKGFVYMEADKQCDINEACKGLTGIYVTRVQPVPPNEVYHLFSVRKKSPDISE 238
           SAFAVDH+KGF+Y+EA++Q DIN+AC G+TGIY TRVQPVP NEV+HLFSV+ K P+ISE
Sbjct: 181 SAFAVDHVKGFIYVEAERQNDINQACYGITGIYATRVQPVPRNEVFHLFSVKIKKPEISE 240

Query: 239 GMRARIKSGNYKGDLPQVAAVS---KKTTVKLIPRVDLQALAAKFGGGNSRQKTAIAAPR 295
           GM  R+K GNYKGDL QV AV+   KK TVKL+PR+DL ALAAKFGGG  RQK A+ APR
Sbjct: 241 GMWGRVKGGNYKGDLAQVVAVNNTHKKVTVKLVPRIDLFALAAKFGGG-CRQKAAVPAPR 299

Query: 296 LISSSELDEFRPLIQLKRDRDTGKVFEVLDGMMFKDGFVYKKVSPDSLSLWGVVPTEEEL 355
           LISS+EL+EFRPLIQ+K DR+TGKVF+VLDGM+ KDGF+YKKVSPDSLS WGVVPTEEEL
Sbjct: 300 LISSTELEEFRPLIQIKHDRETGKVFQVLDGMLLKDGFLYKKVSPDSLSFWGVVPTEEEL 359

Query: 356 LKFGTSENNESTDLEWLSQLYGDSKKKRVVRGADXXXXXXXXXXXXXVVNGFELNDLVCF 415
           LKFG+SENN+S D+EWLS +YGD+KKKRV   AD               N FEL  LV F
Sbjct: 360 LKFGSSENNDSNDMEWLSNIYGDTKKKRVTI-ADKGDGKGEGSSASGAGNDFELYALVRF 418

Query: 416 GKKDFGVIVSMDKEDCYKILKEGSDGPVAVTLERHQIKSGLFDLKLTAQDRHSKTILVND 475
            KKDFGVI+S DK+  YKILKE S+GPVAVT++R++IKS L DLKL+AQD H KTI+VND
Sbjct: 419 RKKDFGVIISTDKDGTYKILKESSEGPVAVTVQRNEIKSALCDLKLSAQDLHRKTIVVND 478

Query: 476 NVRVLEGPSKDKQGIVKHIYRGIVFLYNEIEEENCGYFTAKSSVCEKVKHGVAD--CIGK 533
           NV+VL+GPS+ KQGIV+HIY+GIVFLY+  EEEN GY TAK+ +CEKVK  V+D   IGK
Sbjct: 479 NVKVLDGPSQGKQGIVRHIYQGIVFLYDGNEEENGGYVTAKAIMCEKVKLAVSDFSAIGK 538

Query: 534 DGEPGPLIFEDPLEDPPTSPRSPLSLKKPWQARENNREFNRGDQDSLFAIGQTLRIRIGP 593
           D EPGPL+F+D      +SPRSPLS KKPWQA+E  ++FNRGD DSLFAIGQTLRIRIGP
Sbjct: 539 DSEPGPLVFDDQP----SSPRSPLSPKKPWQAKE--KDFNRGDSDSLFAIGQTLRIRIGP 592

Query: 594 LKGYLCRVIAIRRSDVTVKLDSQQKVLTVKSEHLSEVQGKXXXXXXXXXXXXXXLKPFDL 653
           LKGYLCRV  IRRSDVTVKLDSQQK LTVKSEHLS VQGK              L PFDL
Sbjct: 593 LKGYLCRVTGIRRSDVTVKLDSQQKFLTVKSEHLSVVQGKSTAVSASGDPDSSSLNPFDL 652

Query: 654 LGTE 657
           LG E
Sbjct: 653 LGAE 656


>Medtr3g089910.1 | transcription elongation factor SPT5-like
           protein, putative | HC | chr3:40863011-40870564 |
           20130731
          Length = 1040

 Score =  200 bits (508), Expect = 5e-51,   Method: Compositional matrix adjust.
 Identities = 115/292 (39%), Positives = 174/292 (59%), Gaps = 14/292 (4%)

Query: 100 EEFDRFLEERYAGGSKFSHFADEYDDKPMDPSSMHPGAKEVTPAIWKVKCTVGRERLSAF 159
           EE  R ++ERY G  + + + +E  D  ++  ++ P  ++  P +W VKC +GRER +A 
Sbjct: 147 EEMARRIQERY-GKQRLAEYDEETTD--VEQQALLPSVRD--PKLWMVKCAIGRERETAV 201

Query: 160 CLMQKFADMKKLGTNLKLISAFAVDHMKGFVYMEADKQCDINEACKGLTGIYVTRVQPVP 219
           CLMQK+ D    G+ L++ SA A+DH+K ++Y+EADK+  + EACKGL  I+  ++  VP
Sbjct: 202 CLMQKYIDK---GSELQIRSAIALDHLKNYIYVEADKEAHVREACKGLRNIFGQKITLVP 258

Query: 220 PNEVYHLFSVRKKSPDISEGMRARIKSGNYKGDLPQVA---AVSKKTTVKLIPRVDLQAL 276
             E+  + SV  K+ D++     R+K G YKGDL +V     V ++  VKLIPR+DLQAL
Sbjct: 259 IREMTDVLSVESKAIDLARDTWVRMKIGTYKGDLAKVVDVDNVRQRVRVKLIPRIDLQAL 318

Query: 277 AAKFGGGNSRQKTA-IAAPRLISSSELDEFRPLIQLKRDRDTGKVFEVLDGMMFKDGFVY 335
           A K  G    +K A +  PR ++  E  E    ++ +RD   G+ F+ + GMMFKDGF+Y
Sbjct: 319 ANKLEGREVVKKKAFVPPPRFMNVEEARELHIRVEHRRDATGGERFDTIGGMMFKDGFLY 378

Query: 336 KKVSPDSLSLWGVVPTEEELLKFGTSENNESTDLEWLSQLYGDSKKKRVVRG 387
           K VS  SL    + PT +EL KF   +  E+ D+  LS L+ + KK   ++G
Sbjct: 379 KSVSIKSLYSQNIKPTFDELEKF--RKPGETGDVASLSTLFANRKKGHFMKG 428



 Score =  116 bits (291), Expect = 8e-26,   Method: Compositional matrix adjust.
 Identities = 87/262 (33%), Positives = 131/262 (50%), Gaps = 20/262 (7%)

Query: 375 LYGDSKKKRVVRGADXXXXXXXXXXXXXVVNGFELNDLVCFGKKDFGVIVSMDKEDCYKI 434
           L  D+ K+ +   AD              +  +EL DLV      FGVI+ ++ E  +++
Sbjct: 507 LISDTTKEHIRAFADDVVESSEVTTGVTKIGDYELRDLVLLDNSSFGVIIRVESE-AFQV 565

Query: 435 LKEGSDGPVAVTLERHQIKSGLFDLKLTAQDRHSKTILVNDNVRVLEGPSKDKQGIVKHI 494
           LK  +D P  V ++  +IK  L + K+  QD+   T+   D VR+LEGP K  QG V+HI
Sbjct: 566 LKGVTDRPEVVLVKLREIKCKL-EKKINVQDKFRNTVSSKDVVRILEGPCKGNQGSVEHI 624

Query: 495 YRGIVFLYNEIEEENCGYFTAKSSVCEKVKHGVADCIGKDGE-----PGPLIFEDPLEDP 549
           YRG++F+++    E+ G+   K+  C  V  G      ++G+     PG       L  P
Sbjct: 625 YRGVLFVFDRHHLEHAGFMCVKAQSCVVVG-GSRSNSDRNGDVHSRFPG-------LRTP 676

Query: 550 PTSPRSP--LSLKKPWQARENNREFNRGDQDSLFAIGQTLRIRIGPLKGYLCRVIAIRRS 607
           P  P+SP   S   P  A   +    R   D L   G T+++R G  KGY  RVI ++ S
Sbjct: 677 PRIPQSPHRFSRGGPPSAGGRHNRGGR-GHDGL--TGATVKVRQGSYKGYRGRVIEVKGS 733

Query: 608 DVTVKLDSQQKVLTVKSEHLSE 629
            V V+L+SQ KV+TV   H+S+
Sbjct: 734 FVRVELESQMKVVTVDRNHISD 755


>Medtr1g023975.1 | global transcription factor group protein | HC |
           chr1:7742965-7743951 | 20130731
          Length = 193

 Score =  129 bits (323), Expect = 1e-29,   Method: Composition-based stats.
 Identities = 77/188 (40%), Positives = 112/188 (59%), Gaps = 17/188 (9%)

Query: 103 DRFLEERYAGGSKFSHFADEYDDKPMD--PSSMHPGAKEVTPAIWKVKCTVGRERLSAFC 160
           +R + ERY         A+EYD++  D    S+ P  ++  P +W VKC +GRER SA C
Sbjct: 2   ERRIHERYGK----QRLAEEYDEETTDVEQQSLLPSVRD--PKLWMVKCVIGRERESAVC 55

Query: 161 LMQKFADMKKLGTNLKLISAFAVDHMKGFVYMEADKQCDINEA--CKGLTGIYVTR-VQP 217
           LMQK+ +    G++L++ SA A+DH+K ++Y+EADK+  + E   C   +  Y+ R +  
Sbjct: 56  LMQKYINK---GSDLQIRSAIALDHLKNYIYVEADKEAHVREVLYCLQRSSQYIGRQITL 112

Query: 218 VPPNEVYHLFSVRKKSPDISEGMRARIKSGNYKGDLPQVAAVS---KKTTVKLIPRVDLQ 274
           VP  E+  + SV  K+ D++     R+K G YKGDL +V  V    +K TVKLIPR+DLQ
Sbjct: 113 VPIREMTDVLSVESKAIDLARDTWIRMKIGTYKGDLAKVVNVDNVRQKVTVKLIPRIDLQ 172

Query: 275 ALAAKFGG 282
           ALA K  G
Sbjct: 173 ALANKLEG 180


>Medtr5g062110.1 | hypothetical protein | LC |
           chr5:25797247-25799563 | 20130731
          Length = 304

 Score =  114 bits (285), Expect = 4e-25,   Method: Compositional matrix adjust.
 Identities = 60/108 (55%), Positives = 71/108 (65%), Gaps = 1/108 (0%)

Query: 327 MMFKDGFVYKKVSPDSLSLWGVVPTEEELLKFGTSENNESTDLEWLSQLYGDSKKKRVVR 386
           M+ KDGF+YKKVS DSL+ W VVPTEEELLKFG S+N+ES D+EWLS +YGDSKKKRV+ 
Sbjct: 1   MLLKDGFLYKKVSLDSLNFWDVVPTEEELLKFGPSKNSESNDMEWLSNIYGDSKKKRVII 60

Query: 387 GADXXXXXXXXXXXXXVVNGFELNDLVCFGKKDFG-VIVSMDKEDCYK 433
                             N FEL  LV F KK+   VI+S DK+  YK
Sbjct: 61  AEKGGDGKGEGSLASGAGNDFELYALVRFRKKEENCVIISTDKDGTYK 108


>Medtr7g050470.1 | transcription elongation factor SPT5-like
           protein, putative | LC | chr7:16835096-16837074 |
           20130731
          Length = 241

 Score = 77.8 bits (190), Expect = 4e-14,   Method: Compositional matrix adjust.
 Identities = 46/135 (34%), Positives = 73/135 (54%), Gaps = 10/135 (7%)

Query: 142 PAIWKVKCTVGRERLSAFCLMQKFADMKKLGTNLKLISAFAVDHMKGFVYMEADKQCDIN 201
           P +WKV+CT GRE+ +   LMQK   M+K      + SAF V+ +   VY+EA K+ D+ 
Sbjct: 35  PKLWKVQCTAGREKEAVIRLMQK--RMQKC----TIFSAFVVESLPKCVYVEAHKESDVK 88

Query: 202 EACKGLTGIYVTRVQPVPPNEVYHLFSVRKKSPDISEGMRARIKSGN----YKGDLPQVA 257
           EACKG+  +Y  ++  VP NE+  L ++  KS    +      +SG      KGD+  V 
Sbjct: 89  EACKGVQTLYCKKIIAVPMNEMNDLMTLASKSNKEEQNFTQSGESGKKQQFRKGDIVMVT 148

Query: 258 AVSKKTTVKLIPRVD 272
               +  +K++ +V+
Sbjct: 149 DGETEGKIKVVDKVE 163