Miyakogusa Predicted Gene

Lj5g3v2056240.2

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v2056240.2 Non Characterized Hit- tr|I3SY33|I3SY33_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,100,0,seg,NULL;
VACUOLE MEMBRANE PROTEIN 1,NULL; MEMBRANE-ASSOCIATED PROGESTERONE
RECEPTOR COMPONENT-RELAT,CUFF.56560.2
         (412 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g104680.1 | vacuole membrane-like protein | HC | chr1:4714...   633   0.0  
Medtr5g006110.1 | vacuole membrane-like protein | HC | chr5:7765...   585   e-167
Medtr5g006110.3 | vacuole membrane-like protein | HC | chr5:7765...   501   e-142
Medtr5g006110.2 | vacuole membrane-like protein | HC | chr5:7765...   499   e-141

>Medtr1g104680.1 | vacuole membrane-like protein | HC |
           chr1:47149684-47156443 | 20130731
          Length = 418

 Score =  633 bits (1632), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 306/392 (78%), Positives = 329/392 (83%), Gaps = 2/392 (0%)

Query: 21  LHKKHQXXXXXXXXXXQPFKTLKFFTFSVIQYLKKTAFYXXXXXXXXXXXSFVVGALGIM 80
           L +KHQ          QPFKTLKFFT ++IQYLKKTA Y           S VVGALGIM
Sbjct: 20  LIEKHQLELDNLTLTTQPFKTLKFFTLAIIQYLKKTATYLLAKGGLLMLLSVVVGALGIM 79

Query: 81  LITFDGPHEKHLEELLKYFRFGLWWVVXXXXXXXXXXXXXHTFVLYLGPHIAFFTLKAMQ 140
           LIT +GPHEKH EELLKYFRFGLWW+              HTFVLYLGPHIAFFT+KAMQ
Sbjct: 80  LITVEGPHEKHFEELLKYFRFGLWWITLGIASSIGLGSGLHTFVLYLGPHIAFFTIKAMQ 139

Query: 141 CGRVDLKSAPYDTIQLDRGPSWLDKDCSQFGPPLL--EYSSHIPLSSILSQVVLEAVLWG 198
           CGRVDLKSAPYDTIQLDRGP+WLD DCSQFGPP+   ++ S IPLS IL QV LEAVLWG
Sbjct: 140 CGRVDLKSAPYDTIQLDRGPTWLDMDCSQFGPPMFQSDHGSQIPLSIILIQVQLEAVLWG 199

Query: 199 MGTAIGELPPYFISRAARLSGSKLDSIEELDTENNGIMATYVNKIKRWFLSHTQHLNFLT 258
           MGTAIGELPPYFISRAARLSGSKLD++EELDTEN GI+ TY+N+IKRWFLSH  HLNFLT
Sbjct: 200 MGTAIGELPPYFISRAARLSGSKLDAMEELDTENEGIITTYLNRIKRWFLSHCHHLNFLT 259

Query: 259 IMALASVPNPLFDLAGIMCGQFGIPFWKFFLATFIGKAIIKTHIQTVFIISVCNNQLLNW 318
           I+ALASVPNPLFDLAGIMCGQFG+PFWKFFLATFIGKA+IKTHIQTVFIISVCNNQLLNW
Sbjct: 260 ILALASVPNPLFDLAGIMCGQFGVPFWKFFLATFIGKALIKTHIQTVFIISVCNNQLLNW 319

Query: 319 IENEFIWVLGHIPGFESILPSLIAKLHAAKDKYLKASHPVSPNIQGKKWDFSIASVWNTV 378
           IENEFIWVLGHIP F SILPS+IA LHA KDKYLKA H VSP I+G +WDFSIAS+WNTV
Sbjct: 320 IENEFIWVLGHIPAFASILPSVIANLHAVKDKYLKAPHQVSPKIKGTRWDFSIASIWNTV 379

Query: 379 VWLMLMNFFVKIVNSTAQSYVKKQQEKELAAS 410
           VWLMLMNFFVKIVNSTAQSY+KKQQE ELAAS
Sbjct: 380 VWLMLMNFFVKIVNSTAQSYLKKQQEIELAAS 411


>Medtr5g006110.1 | vacuole membrane-like protein | HC |
           chr5:776561-770690 | 20130731
          Length = 417

 Score =  585 bits (1507), Expect = e-167,   Method: Compositional matrix adjust.
 Identities = 280/394 (71%), Positives = 316/394 (80%), Gaps = 6/394 (1%)

Query: 21  LHKKHQXXXXXXXXXXQPFKTLKFFTFSVIQYLKKTAFYXXXXXXXXXXXSFVVGALGIM 80
           LH+KH+          QPFKTLK+ T +VIQY+KKT  Y           S  VG LG++
Sbjct: 21  LHEKHKMELENLTLTTQPFKTLKYSTLAVIQYIKKTMLYLLAKGGWLMLFSVAVGTLGVV 80

Query: 81  LITFDGPHEKHLEELLKYFRFGLWWVVXXXXXXXXXXXXXHTFVLYLGPHIAFFTLKAMQ 140
           L+T    HEKHLEE L+YFRFGLWWV              HTFVLYLGPHIA FT+KAMQ
Sbjct: 81  LMTLGCLHEKHLEEFLEYFRFGLWWVALGVASSIGLGSGLHTFVLYLGPHIALFTIKAMQ 140

Query: 141 CGRVDLKSAPYDTIQLDRGPSWLDKDCSQFGPPLL--EYSSHIPLSSILSQVVLEAVLWG 198
           CGRVDLKSAPYDTIQL+RGPSWLDK+CSQFGPPL   EY + +PLSSIL QV +EAVLWG
Sbjct: 141 CGRVDLKSAPYDTIQLNRGPSWLDKNCSQFGPPLFQSEYGTRVPLSSILPQVQMEAVLWG 200

Query: 199 MGTAIGELPPYFISRAARLSGSKLDSIEELDTENNGIMATYVNKIKRWFLSHTQHLNFLT 258
           +GTAIGELPPYFISRAARLSGS++D++EELD+E+ G++    N+IK WF SHTQHLNF T
Sbjct: 201 LGTAIGELPPYFISRAARLSGSRMDAMEELDSEDKGVL----NQIKCWFFSHTQHLNFFT 256

Query: 259 IMALASVPNPLFDLAGIMCGQFGIPFWKFFLATFIGKAIIKTHIQTVFIISVCNNQLLNW 318
           I+ LASVPNPLFDLAGIMCGQFGIPFW FFLAT IGKAIIKTHIQTVFIISVCNNQLL+W
Sbjct: 257 ILVLASVPNPLFDLAGIMCGQFGIPFWNFFLATLIGKAIIKTHIQTVFIISVCNNQLLDW 316

Query: 319 IENEFIWVLGHIPGFESILPSLIAKLHAAKDKYLKASHPVSPNIQGKKWDFSIASVWNTV 378
           IENEFIWVL HIPGF S+LP L A LHA KDKYLKA HPVSPN +GKKWDFS  S+WNTV
Sbjct: 317 IENEFIWVLSHIPGFASVLPKLTANLHAMKDKYLKAPHPVSPNTKGKKWDFSFTSIWNTV 376

Query: 379 VWLMLMNFFVKIVNSTAQSYVKKQQEKELAASTE 412
           VWLMLMNFF+KIVNSTAQ+++KKQQE E+AA T+
Sbjct: 377 VWLMLMNFFIKIVNSTAQTHLKKQQESEVAALTK 410


>Medtr5g006110.3 | vacuole membrane-like protein | HC |
           chr5:776561-770690 | 20130731
          Length = 313

 Score =  501 bits (1290), Expect = e-142,   Method: Compositional matrix adjust.
 Identities = 236/294 (80%), Positives = 263/294 (89%), Gaps = 6/294 (2%)

Query: 121 HTFVLYLGPHIAFFTLKAMQCGRVDLKSAPYDTIQLDRGPSWLDKDCSQFGPPLL--EYS 178
           HTFVLYLGPHIA FT+KAMQCGRVDLKSAPYDTIQL+RGPSWLDK+CSQFGPPL   EY 
Sbjct: 17  HTFVLYLGPHIALFTIKAMQCGRVDLKSAPYDTIQLNRGPSWLDKNCSQFGPPLFQSEYG 76

Query: 179 SHIPLSSILSQVVLEAVLWGMGTAIGELPPYFISRAARLSGSKLDSIEELDTENNGIMAT 238
           + +PLSSIL QV +EAVLWG+GTAIGELPPYFISRAARLSGS++D++EELD+E+ G++  
Sbjct: 77  TRVPLSSILPQVQMEAVLWGLGTAIGELPPYFISRAARLSGSRMDAMEELDSEDKGVL-- 134

Query: 239 YVNKIKRWFLSHTQHLNFLTIMALASVPNPLFDLAGIMCGQFGIPFWKFFLATFIGKAII 298
             N+IK WF SHTQHLNF TI+ LASVPNPLFDLAGIMCGQFGIPFW FFLAT IGKAII
Sbjct: 135 --NQIKCWFFSHTQHLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWNFFLATLIGKAII 192

Query: 299 KTHIQTVFIISVCNNQLLNWIENEFIWVLGHIPGFESILPSLIAKLHAAKDKYLKASHPV 358
           KTHIQTVFIISVCNNQLL+WIENEFIWVL HIPGF S+LP L A LHA KDKYLKA HPV
Sbjct: 193 KTHIQTVFIISVCNNQLLDWIENEFIWVLSHIPGFASVLPKLTANLHAMKDKYLKAPHPV 252

Query: 359 SPNIQGKKWDFSIASVWNTVVWLMLMNFFVKIVNSTAQSYVKKQQEKELAASTE 412
           SPN +GKKWDFS  S+WNTVVWLMLMNFF+KIVNSTAQ+++KKQQE E+AA T+
Sbjct: 253 SPNTKGKKWDFSFTSIWNTVVWLMLMNFFIKIVNSTAQTHLKKQQESEVAALTK 306


>Medtr5g006110.2 | vacuole membrane-like protein | HC |
           chr5:776561-770690 | 20130731
          Length = 306

 Score =  499 bits (1286), Expect = e-141,   Method: Compositional matrix adjust.
 Identities = 236/294 (80%), Positives = 263/294 (89%), Gaps = 6/294 (2%)

Query: 121 HTFVLYLGPHIAFFTLKAMQCGRVDLKSAPYDTIQLDRGPSWLDKDCSQFGPPLL--EYS 178
           HTFVLYLGPHIA FT+KAMQCGRVDLKSAPYDTIQL+RGPSWLDK+CSQFGPPL   EY 
Sbjct: 10  HTFVLYLGPHIALFTIKAMQCGRVDLKSAPYDTIQLNRGPSWLDKNCSQFGPPLFQSEYG 69

Query: 179 SHIPLSSILSQVVLEAVLWGMGTAIGELPPYFISRAARLSGSKLDSIEELDTENNGIMAT 238
           + +PLSSIL QV +EAVLWG+GTAIGELPPYFISRAARLSGS++D++EELD+E+ G++  
Sbjct: 70  TRVPLSSILPQVQMEAVLWGLGTAIGELPPYFISRAARLSGSRMDAMEELDSEDKGVL-- 127

Query: 239 YVNKIKRWFLSHTQHLNFLTIMALASVPNPLFDLAGIMCGQFGIPFWKFFLATFIGKAII 298
             N+IK WF SHTQHLNF TI+ LASVPNPLFDLAGIMCGQFGIPFW FFLAT IGKAII
Sbjct: 128 --NQIKCWFFSHTQHLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWNFFLATLIGKAII 185

Query: 299 KTHIQTVFIISVCNNQLLNWIENEFIWVLGHIPGFESILPSLIAKLHAAKDKYLKASHPV 358
           KTHIQTVFIISVCNNQLL+WIENEFIWVL HIPGF S+LP L A LHA KDKYLKA HPV
Sbjct: 186 KTHIQTVFIISVCNNQLLDWIENEFIWVLSHIPGFASVLPKLTANLHAMKDKYLKAPHPV 245

Query: 359 SPNIQGKKWDFSIASVWNTVVWLMLMNFFVKIVNSTAQSYVKKQQEKELAASTE 412
           SPN +GKKWDFS  S+WNTVVWLMLMNFF+KIVNSTAQ+++KKQQE E+AA T+
Sbjct: 246 SPNTKGKKWDFSFTSIWNTVVWLMLMNFFIKIVNSTAQTHLKKQQESEVAALTK 299