Miyakogusa Predicted Gene

Lj5g3v2045280.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v2045280.1 Non Characterized Hit- tr|I1JRJ1|I1JRJ1_SOYBN
Uncharacterized protein OS=Glycine max PE=4 SV=1,83.57,0,EUKARIOTIC
TRANSLATION INITIATION FACTOR 2B, EPSILON SUBUNIT,NULL;
SUGAR-1-PHOSPHATE GUANYL TRANSFER,CUFF.56520.1
         (722 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr7g115610.1 | initiation factor-2Bepsilon-like protein | HC ...  1209   0.0  
Medtr6g023790.1 | eIF4-gamma/eIF5/eIF2-epsilon domain protein | ...   153   5e-37
Medtr8g085640.1 | translation initiation factor eIF-2B gamma sub...    86   1e-16
Medtr5g080770.1 | mannose-1-phosphate guanylyltransferase-like p...    54   4e-07

>Medtr7g115610.1 | initiation factor-2Bepsilon-like protein | HC |
           chr7:47797427-47785063 | 20130731
          Length = 722

 Score = 1209 bits (3128), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 586/715 (81%), Positives = 631/715 (88%), Gaps = 1/715 (0%)

Query: 1   MGAPKKSSARVSEDPDDLVRVPWQAILLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60
           MGAPKKS+ARVSED D+LVRVP QAILLADSFTTKFRPITLERPKVLLPL NVPMINYTL
Sbjct: 1   MGAPKKSAARVSEDHDELVRVPLQAILLADSFTTKFRPITLERPKVLLPLANVPMINYTL 60

Query: 61  TWLESAGVEEVFVFCCAHAKQVIQYLDKSEWLSQPNFTVTTIESQNSVSAGDALRVIYER 120
           TWLESAGVEEVFVFCCAHAKQVI YL+KSE+ +QPNFTVTTIESQN VSAGDALRVIYER
Sbjct: 61  TWLESAGVEEVFVFCCAHAKQVINYLEKSEF-NQPNFTVTTIESQNCVSAGDALRVIYER 119

Query: 121 NVIHGDFVLISGDTVSNMSLTQALLEHKERKKKDSNAVMTMVIKRSKPNQAIHQSRLGTD 180
           NVI GDFVLISGDTVSNMSLTQALLEHKERKKKDSNAVMTMVIKRSK + AIHQSRLGTD
Sbjct: 120 NVIQGDFVLISGDTVSNMSLTQALLEHKERKKKDSNAVMTMVIKRSKTHPAIHQSRLGTD 179

Query: 181 EIFMAIDPDTKQLLYYEDKADYSKSTLHLDKSLLVDNPSLSLHHDKQDCYIDICSPEVLS 240
           EIFMAIDP++KQLLYYEDKAD+SK TLHL+ SLL DNPSLSLHHDKQDCYIDICSPEVLS
Sbjct: 180 EIFMAIDPNSKQLLYYEDKADFSKGTLHLENSLLADNPSLSLHHDKQDCYIDICSPEVLS 239

Query: 241 LFTDNFDYQHLRRHFVKGLLVDDIMGYKIFVHEIHSDYAARIDNFRSYDTVSKDIIHRWT 300
           LFTDNFDYQHLRRHFVKGLL+DDIMGYKIFVHEI +DYAARIDNFRSYDTVSKDIIHRWT
Sbjct: 240 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIFVHEIRADYAARIDNFRSYDTVSKDIIHRWT 299

Query: 301 YPFVPDVMNFGKTATKLERQGMYRASEISQSQSAVIGPSTAIGSGTKIGYNTKISNSIIG 360
           YP VPDVMNFG TATKLERQG+YR S+ISQ+QSAV+GP + IGSGTKIG NTKI NS++G
Sbjct: 300 YPLVPDVMNFGNTATKLERQGIYRGSDISQAQSAVVGPFSVIGSGTKIGKNTKILNSVVG 359

Query: 361 EGCKIGSDVLIEGCYIWDNVTIEDGCKLWHAIVCDGATMKSGAVLEPGVVLSFKVVVGQG 420
            GCKIGS+V IEGCYIWDNVTIEDGC+L HAIVC+G  MKSG+ LEPGVVLSFKVVVGQ 
Sbjct: 360 IGCKIGSNVHIEGCYIWDNVTIEDGCELRHAIVCEGVIMKSGSALEPGVVLSFKVVVGQE 419

Query: 421 FVVPPYSKVSLLQQPIEEDSDEELEYADSASAITSTVDKSDVEIAYQELDTHFSPPSELG 480
           FVVPPYS+VSLLQQP+EEDSDEELEYADS SAITSTVDKSD E A + LD HF+P SELG
Sbjct: 420 FVVPPYSQVSLLQQPVEEDSDEELEYADSTSAITSTVDKSDGETASELLDGHFTPASELG 479

Query: 481 MGGAGYVWPKCEGGHEEEWRHSVAPITADKILEALKAIEDDLELTHDDTILPPSGELKPX 540
            GGAGYVWPKCEGGHEEEWRHSVAPIT DKILEA+KA+ED+LEL HD +ILPPSGEL P 
Sbjct: 480 FGGAGYVWPKCEGGHEEEWRHSVAPITEDKILEAVKAMEDELELIHDGSILPPSGELIPN 539

Query: 541 XXXXXXXXXXXXXXXXXKEVEATFLRGVDENIQDNHLILEVNSLKLSYNKVTADCAGAVF 600
                            KEVEATFLR V ENIQ+NHL LEVNSLKLSYNKV ADCAGA+F
Sbjct: 540 PNDSDDDDNDDLRDDFDKEVEATFLRAVLENIQENHLTLEVNSLKLSYNKVAADCAGALF 599

Query: 601 YAMMKHAIDMPHSSADSLMKNVQDVLKQWKNVLAPYLSDMDEQIEVILKLEEMCLESAKE 660
           YAMMK+A+D+PHSSAD L++NV  VL +WK VLA YL+D DEQIEVILK EE+CLESAKE
Sbjct: 600 YAMMKYAVDLPHSSADGLVQNVHVVLTKWKKVLASYLNDTDEQIEVILKFEEICLESAKE 659

Query: 661 YAPLFTKILHFMYEEDIVDEDAILSWXXXXXXXXXXXXVFVKQAQKLIQWLKEAP 715
           +APLFTKILH+MY EDI++EDAILSW            VFV QAQ LIQWLKEAP
Sbjct: 660 FAPLFTKILHYMYNEDIIEEDAILSWEDEKKDADEADKVFVNQAQPLIQWLKEAP 714


>Medtr6g023790.1 | eIF4-gamma/eIF5/eIF2-epsilon domain protein | LC
           | chr6:8363733-8368854 | 20130731
          Length = 270

 Score =  153 bits (387), Expect = 5e-37,   Method: Compositional matrix adjust.
 Identities = 79/136 (58%), Positives = 98/136 (72%), Gaps = 4/136 (2%)

Query: 581 VNSLKLSYNKVTADCAGAVFYAMMKHAIDMPHSSADSLMKNVQDVLKQWKNVLAPYLSDM 640
           +N+ KLSYNK+ ADCAGA+FYAMMK+A+DMPHSSA   ++    +L ++  +L    + +
Sbjct: 130 LNAPKLSYNKIAADCAGALFYAMMKYAVDMPHSSA---VQKCTSILFKFYLILEFSETTV 186

Query: 641 DE-QIEVILKLEEMCLESAKEYAPLFTKILHFMYEEDIVDEDAILSWXXXXXXXXXXXXV 699
               IEVILK EEMCLESAKE+APLFTKILH+MY ED+++EDAILSW            V
Sbjct: 187 KALAIEVILKFEEMCLESAKEFAPLFTKILHYMYNEDVIEEDAILSWEDEKKDPDEADKV 246

Query: 700 FVKQAQKLIQWLKEAP 715
           FV  AQ LIQWLKEAP
Sbjct: 247 FVNLAQPLIQWLKEAP 262



 Score = 67.4 bits (163), Expect = 4e-11,   Method: Compositional matrix adjust.
 Identities = 37/67 (55%), Positives = 44/67 (65%), Gaps = 10/67 (14%)

Query: 1   MGAPKKSSARVSEDPDDLVRVPWQAILLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60
           MGAPKKS+ARVSED D+LVRVP QAI+LADSFTTK            L L+N P ++Y  
Sbjct: 90  MGAPKKSAARVSEDHDELVRVPLQAIMLADSFTTKLD----------LSLLNAPKLSYNK 139

Query: 61  TWLESAG 67
              + AG
Sbjct: 140 IAADCAG 146


>Medtr8g085640.1 | translation initiation factor eIF-2B gamma
           subunit | HC | chr8:35495862-35491497 | 20130731
          Length = 467

 Score = 85.9 bits (211), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 105/502 (20%), Positives = 196/502 (39%), Gaps = 121/502 (24%)

Query: 23  WQAILLADSFTTKFRP-ITLERPKVLLPLVNVPMINYTLTWLESAGVEEVFVFCCAHAKQ 81
           +Q ++LA   + K  P ++ E P  LLP+ N P+++Y +  LE + ++++ V      K 
Sbjct: 3   FQVVVLAGGVSKKLLPLVSHELPNALLPVANRPVLSYVVELLELSNLKDLIVV--VEGKD 60

Query: 82  VIQYLDKSEWLSQP---NFTVTTIESQNSVSAGDALRVIYERNVIHGDFVLISGDTVSNM 138
               L+   W+S        V        V    A+R I  R++   D +++SGD VS++
Sbjct: 61  T--ALNVGAWISGAYADRLHVEVAAVPEDVGTAGAIRAI-ARHLYAKDILIVSGDLVSDV 117

Query: 139 SLTQALLEHKERKKKDSNAVMTMVIKR---SKPNQAIHQSRLGTDEI-------FMAIDP 188
            +      H+       +AV+T ++     S P +++  S  G D+         + +DP
Sbjct: 118 PIGAVAATHRRH-----DAVVTALLCNTPVSGPLESV--SSGGKDKAKKPGRYDLIGLDP 170

Query: 189 DTKQLLYYEDKADYSKSTLHLDKSLLVDNPSLSLHHDKQDCYIDICSPEVLSLFTDNFDY 248
             + LL+    A+  K  L + KS+L     + +  D  D ++      VL    D    
Sbjct: 171 TKQFLLHIATGAEVEKD-LRIQKSILRSVGQVEIRADLMDAHLYAFKRSVLLEVLDQNGE 229

Query: 249 QHLRRHFVKGLLVDDIMGYKIFV------------------------------------- 271
            H  +H V   LV   +  ++ +                                     
Sbjct: 230 FHSLKHDVLPYLVRSQLKSEVLLNGTPQAEENGTEKVISQSNQQMLSQILANASEPTFHL 289

Query: 272 -HEIHSD--------------------YAARIDNFRSYDTVSKDII----HRWTYPFVPD 306
            HE+ ++                    Y AR+++ ++Y+ +++D+I    H   Y F   
Sbjct: 290 RHELSTNGSDSVRRTHKCCVYIAGSNKYCARLNSLQAYNDINRDVIGEASHLSGYSFSSH 349

Query: 307 VMNFGKTATKLERQGMYRASEISQSQSAVIGPSTAIGSGTKIGYNTKISNSIIGEGCKIG 366
                 TA            E+    +  +GP   +G G+++G    +  S+IG  C+IG
Sbjct: 350 NNIIDPTA------------ELGAKTT--VGPHCMLGEGSQMGDKCSVKRSVIGRHCRIG 395

Query: 367 SDVLIEGCYIWDNVTIEDGCKLWHAIVCDGATMKSGAVLEPGVVLSFKVVVGQGFVVPPY 426
           ++V +    + ++VTI DGC +  +++C    ++  A L+          VG G++V   
Sbjct: 396 ANVKVVNSVVMNHVTIGDGCSIQGSVICSNVQLQERATLK-------DCQVGAGYMVTAG 448

Query: 427 SKVSLLQQPIEEDSDEELEYAD 448
           S           D   EL + D
Sbjct: 449 S-----------DCKGELRFGD 459


>Medtr5g080770.1 | mannose-1-phosphate guanylyltransferase-like
           protein | HC | chr5:34582070-34586696 | 20130731
          Length = 361

 Score = 54.3 bits (129), Expect = 4e-07,   Method: Compositional matrix adjust.
 Identities = 94/402 (23%), Positives = 153/402 (38%), Gaps = 92/402 (22%)

Query: 24  QAILLADSFTTKFRPITLERPKVLLPLVNVPMINYTLTWLESAGVEEVFVFCCAHAKQVI 83
           +A++L   F T+ RP+TL  PK L+   N PMI + +  L++ GV EV +      + ++
Sbjct: 2   KALILVGGFGTRLRPLTLSVPKPLVDFANKPMILHQIEALKATGVTEVVLAINYQPEVML 61

Query: 84  QYLDKSEWLSQPNFTVTTIESQNSVSAGDALRVIYERNVIHGD----FVLISGDTVSNMS 139
            +L   E        +T   SQ +   G A  +   R+ +  D    F +++ D +S+  
Sbjct: 62  NFLKDFE----AKLGITISCSQETEPLGTAGPLALARDKLIDDSGEPFFVLNSDVISDYP 117

Query: 140 LTQALLEHKERKKKDSNAVMTMVIKRSKPNQAIHQSRLGTDEIFMAIDPDTKQLLYYEDK 199
           L + +  HK    + S     MV K  +P      S+ G     + ++  T Q+  + +K
Sbjct: 118 LKEMIEFHKSHGGEAS----IMVTKVDEP------SKYGV----VVMEETTGQVEKFVEK 163

Query: 200 ADYSKSTLHLDKSLLVDNPSLSLHHDKQDCYIDICSPEVLSLFTDNFDYQHLRRHFVKGL 259
                        L V N        K +  I + +P VL       D   LR   ++  
Sbjct: 164 P-----------KLFVGN--------KINAGIYLLNPSVL-------DRIELRPTSIEKE 197

Query: 260 LVDDIMGYKIFVHEIHSDYAARIDNFRSYDTVSKDIIHRWTYPFVPDVMNFGKTATKLER 319
           +   I   K         YA  +  F             W        M+ G+    +  
Sbjct: 198 IFPKIAAEKKL-------YAMVLPGF-------------W--------MDIGQPRDYITG 229

Query: 320 QGMYRASEISQSQSAVIGPSTAIGSGTKIGYNTKISNSI-IGEGCKIGSDVLI-EGCYIW 377
             +Y  S   +S S + G S  +G       N  +  +  IGEGC IG DV I  GC   
Sbjct: 230 LRLYLDSLRKKSSSKLAGGSNIVG-------NVIVDETAKIGEGCLIGPDVAIGPGC--- 279

Query: 378 DNVTIEDGCKLWHAIVCDGATMKSGAVLEPGVVLSFKVVVGQ 419
               +E G +L    V  G  +K  A +   ++  +   VGQ
Sbjct: 280 ---IVESGVRLSRCTVMRGVRIKKHACISSSII-GWHSTVGQ 317