Miyakogusa Predicted Gene

Lj5g3v1889480.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v1889480.2 tr|F6HJC9|F6HJC9_VITVI tRNA pseudouridine
synthase A OS=Vitis vinifera GN=truA PE=3 SV=1,67.51,0,Pseudouridine
synthase,Pseudouridine synthase, catalytic domain; TruA,Pseudouridine
synthase I, TruA,CUFF.56115.2
         (427 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g097390.1 | tRNA pseudouridine synthase A | HC | chr1:4393...   625   e-179
Medtr4g123970.1 | tRNA pseudouridine synthase A | HC | chr4:5111...    93   4e-19
Medtr2g005830.1 | tRNA pseudouridine synthase A | HC | chr2:3378...    64   2e-10
Medtr5g074640.1 | tRNA pseudouridine synthase | HC | chr5:317260...    58   2e-08
Medtr5g074620.1 | tRNA pseudouridine synthase | HC | chr5:317201...    55   1e-07

>Medtr1g097390.1 | tRNA pseudouridine synthase A | HC |
           chr1:43937515-43930054 | 20130731
          Length = 435

 Score =  625 bits (1611), Expect = e-179,   Method: Compositional matrix adjust.
 Identities = 297/386 (76%), Positives = 329/386 (85%), Gaps = 4/386 (1%)

Query: 1   MATQLSDVDHIATLQARVKELEAENAKLLSQLADSHIHEMEKKLHVSGEKN---TGSIKG 57
           MATQLSDVD+I TLQARVKELEAENA LLS+LA     E+ KK++   +K    T     
Sbjct: 9   MATQLSDVDYITTLQARVKELEAENANLLSRLAHCQCSEVGKKVYAPDDKTAEVTEPKIS 68

Query: 58  NRKSEEKIEKMTGYNTRFMSHHSKRYVALKVMYFGKRFYGFASEAQMEPTVESEIFKALA 117
           N KSEE  +K  GY TR++SHHSKRYVALKVMYFGKRFYGF+SEAQMEP+VESE+FKAL 
Sbjct: 69  NGKSEETTKKKPGYKTRYLSHHSKRYVALKVMYFGKRFYGFSSEAQMEPSVESELFKALE 128

Query: 118 KIRLLVGDKKESQYSRCGRTDKGVSSVGQVIALYLRSNLKISGANTGSSGEIV-EEQHEG 176
             RLLVGDKKESQYSRCGRTDKGVSSVGQVIAL+LRS LKISG N GSSGE V EEQ EG
Sbjct: 129 TTRLLVGDKKESQYSRCGRTDKGVSSVGQVIALFLRSKLKISGVNNGSSGEFVSEEQLEG 188

Query: 177 EIDYVRVLNRVLPNDIRILGWCPAPVDFHARFSCLSREYKYFFWKEGLNIPAMESAGIKL 236
           E+DYVRV+NR LP+DIRILGWCP PVDFHARFSC  REY+YFFWKE LNI AME+AG KL
Sbjct: 189 ELDYVRVINRELPSDIRILGWCPVPVDFHARFSCSGREYRYFFWKENLNILAMENAGTKL 248

Query: 237 IGEHDFRNFCKMDATNVHNYRRHITMFEIFPTDVRYGGNQLWVIKIRGSAFLWHQVRCMV 296
           +GEHDFRNFCKMDA NVH Y R ITMFEI+ TDVRY  NQLWVIKIRG AFLWHQVRCMV
Sbjct: 249 VGEHDFRNFCKMDAANVHTYMRRITMFEIYATDVRYEDNQLWVIKIRGRAFLWHQVRCMV 308

Query: 297 AVLFLVGKGLESPNVIDMLLDTNKTPRKPQYIMSSEVPLVLQSCEFEDIKFMCSSDAGEA 356
           AVLF +GKGLESP+VIDMLLDTN+ PRKPQY+M+SE PLVLQSCEFED+KF+CSSD+G+A
Sbjct: 309 AVLFSIGKGLESPDVIDMLLDTNRIPRKPQYVMASEAPLVLQSCEFEDLKFICSSDSGKA 368

Query: 357 LRIHLVNECQTFQLQAAVFHEALLNC 382
           L  HLVNEC+ FQLQ A+FHEA+LNC
Sbjct: 369 LHAHLVNECKIFQLQTAIFHEAILNC 394


>Medtr4g123970.1 | tRNA pseudouridine synthase A | HC |
           chr4:51119303-51121602 | 20130731
          Length = 322

 Score = 93.2 bits (230), Expect = 4e-19,   Method: Compositional matrix adjust.
 Identities = 80/247 (32%), Positives = 115/247 (46%), Gaps = 42/247 (17%)

Query: 86  LKVMYFGKRFYGFASEAQMEPTVESEIFKALAKIRLLVGDKKESQYSRCGRTDKGVSSVG 145
           L + Y G R+ G+  + +  PTV+  + KAL  IR     +K+ Q     RTD GV + G
Sbjct: 46  LLLSYDGTRYAGWQYQ-ESPPTVQCAVEKAL--IRATKLQRKDLQLVGASRTDAGVHAWG 102

Query: 146 QVIALYLRSNLKISGANTGSSGEIVEEQHEGEIDYVRVLNRVLPNDIRILGWCPAPVDFH 205
           QV A +L          T  + + ++  H         LN +LP+DIR+    PA  +FH
Sbjct: 103 QV-AHFL----------TPFNYDNLDSVHAA-------LNGLLPSDIRVREISPASAEFH 144

Query: 206 ARFSCLSREYKYFFWKEG----------------LNIPAMESAGIKLIGEHDFRNFCKMD 249
           ARFS  S+ Y Y  + +                 LN  AM  A    IG+HDF  F    
Sbjct: 145 ARFSVKSKIYHYKIYSDTIMDPFQRHFAYHNMYKLNSAAMREAARYFIGKHDFTAF---- 200

Query: 250 ATNVHNYRRHITMFEIFPTDVRYGGNQLWVIKIRGSAFLWHQVRCMVAVLFLVGKGLESP 309
               HN R    +  IF  DV+  G  L  +++ GS FL+ QVR MVA+L  +GK    P
Sbjct: 201 ENASHNDRIPDPVKHIFRFDVKEMG-ALLQLEVEGSGFLYRQVRNMVALLLQIGKEATPP 259

Query: 310 NVIDMLL 316
           +++  +L
Sbjct: 260 DIVPHIL 266


>Medtr2g005830.1 | tRNA pseudouridine synthase A | HC |
           chr2:337857-333131 | 20130731
          Length = 361

 Score = 64.3 bits (155), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 86/325 (26%), Positives = 125/325 (38%), Gaps = 92/325 (28%)

Query: 78  HHSK--RYVALKVMYFGKRFYGFASEAQMEPTVESEIFKALAKIRLLVGDKKESQYSRCG 135
           HH K  RY+ + V Y G RF+GF  +   + TV   +  A  K    VG       S   
Sbjct: 37  HHRKIQRYL-VAVEYIGTRFFGFQKQL-TDRTVAGVLEDAFCK---FVGQPVSVVCS--S 89

Query: 136 RTDKGVSSVGQVIALYLRSNLKISGANTGSSGEIVEEQHEGEIDYVRVLNRVLP---NDI 192
           RTD GV ++  V       ++ I   +    GE+++  HE  +   R +N  L     D+
Sbjct: 90  RTDAGVHALSNV------CHVDIERISKRKPGEVLQP-HEPAV-VRRAVNHFLQKENGDL 141

Query: 193 RILGWCPAPVDFHARFSCLSREYKYFF---------------W--KEGLNIPAMESAGIK 235
            ++     P DFHAR+    R Y Y                 W   E LN+PAM+ A   
Sbjct: 142 AVIDVRCVPSDFHARYKAQERTYFYRLLSGPETLSTFEKDRAWHIPEELNLPAMQEACKV 201

Query: 236 LIGEHDFRNF----CK-------------------------MDATNVHNYRRHITMFE-- 264
           L+G HDF +F    C+                         MD  N  N    +  F   
Sbjct: 202 LVGHHDFSSFRASGCQAKSPIRSLDELSVVEVTRSPYFPSVMDRENPINVTDDLHCFHSN 261

Query: 265 ---IFP--------------TDVRYGG---NQLWVIKIRGSAFLWHQVRCMVAVLFLVGK 304
              + P               DV +G    ++  V+  R  AFL+HQVR +V VL  VG 
Sbjct: 262 SETVIPLGSSARIDEVSASKEDVGFGKRRYHRCLVVTARARAFLYHQVRLLVGVLKDVGT 321

Query: 305 GLESPNVIDMLLD----TNKTPRKP 325
           G  +   ++ +L+    T  +P  P
Sbjct: 322 GNITVTDVERILNAKTVTATSPMAP 346


>Medtr5g074640.1 | tRNA pseudouridine synthase | HC |
           chr5:31726081-31722203 | 20130731
          Length = 501

 Score = 57.8 bits (138), Expect = 2e-08,   Method: Compositional matrix adjust.
 Identities = 60/236 (25%), Positives = 98/236 (41%), Gaps = 47/236 (19%)

Query: 51  NTGSIKGNRKSEEKIEKMTGYNTRFMSHH----SKRYVALKVMYFGKRFYGFA------S 100
           N+  I GNR+  E   +   +++   S+      K+ V +++ Y G  + G        S
Sbjct: 23  NSTPIIGNRRRWECF-RYCSFSSTPQSYSWQPFRKKKVVMRIAYTGTNYRGLQMQRDQNS 81

Query: 101 EAQMEPTVESEIFKALAKIRLLVGDKKESQYSRCGRTDKGVSSVGQVIALYLRSNLKISG 160
            + +E  +ES IFKA       +GD ++  ++R  RTDKGV S+  ++A  +        
Sbjct: 82  LSTVEKELESAIFKAGGIRESNLGDLEKIGWARSSRTDKGVHSLATMVAFKM-------- 133

Query: 161 ANTGSSGEIVEEQ-HEGEIDY--VRVLNRVLPNDIRILGWCPAPVDFHARFSCLSREYKY 217
                  EI E   +E    +     +N  LP DI+++   PA   F  R  C+ R+Y Y
Sbjct: 134 -------EIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQRSFDPRKECVLRKYSY 186

Query: 218 FFWKEGLNIPAMESAG-------------IKLIGEHDFRNFCKMDATNVHNYRRHI 260
               + + I +  S                +  G H F N+     T+   YRRHI
Sbjct: 187 LLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNY-----TSRSRYRRHI 237


>Medtr5g074620.1 | tRNA pseudouridine synthase | HC |
           chr5:31720119-31716063 | 20130731
          Length = 483

 Score = 55.5 bits (132), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 41/148 (27%), Positives = 62/148 (41%), Gaps = 24/148 (16%)

Query: 81  KRYVALKVMYFGKRFYGFASE------AQMEPTVESEIFKALAKIRLLVGDKKESQYSRC 134
           K+ V ++V Y G  F G   +      + +E  +E+ IFK         GD  +  + R 
Sbjct: 56  KKKVVMRVGYIGTDFRGLQIQRNDHKLSTIEKELETAIFKVGGIRDSNYGDLDKINWGRS 115

Query: 135 GRTDKGVSSVGQVIALYLRSNLKISGANTGSSGEIVEEQHEGE---IDYVRVLNRVLPND 191
            RTDKGV S+  +I+  +               EI E   +G+   I+    +N  LPN 
Sbjct: 116 SRTDKGVHSLSTMISFKM---------------EIPENAWKGDDYGIEMANHINSYLPNS 160

Query: 192 IRILGWCPAPVDFHARFSCLSREYKYFF 219
           IR+    P+   F  R  C  R+Y Y  
Sbjct: 161 IRVFSVLPSTKRFDPRKECSMRKYSYLL 188