Miyakogusa Predicted Gene

Lj5g3v1698870.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v1698870.1 Non Characterized Hit- tr|I1LCL8|I1LCL8_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.5460 PE=,84.46,0,no
description,Immunoglobulin-like fold; no description,Glycoside
hydrolase, catalytic domain; PULLU,CUFF.55789.1
         (939 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g090693.1 | alpha-1,6-glucosidase, pullulanase-type protei...  1581   0.0  
Medtr3g099500.1 | isoamylase-type starch debranching enzyme | HC...   105   1e-22
Medtr3g099500.2 | isoamylase-type starch debranching enzyme | HC...   105   2e-22
Medtr8g101490.1 | isoamylase-type starch debranching enzyme | HC...    85   3e-16
Medtr8g101490.2 | isoamylase-type starch debranching enzyme | HC...    84   5e-16

>Medtr1g090693.1 | alpha-1,6-glucosidase, pullulanase-type protein |
           HC | chr1:40662278-40674399 | 20130731
          Length = 957

 Score = 1581 bits (4093), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 758/871 (87%), Positives = 807/871 (92%), Gaps = 1/871 (0%)

Query: 70  QMQSSLLLYSRAYWVTESVIAWNVDL-GNGFCYLLSSKDASLSIADCKIQGEDLKIKLEE 128
           QMQ +LLLYSRAYW+T+S+IAWNVD   NG CYLLSSKDASL+I++ +IQGEDLKIKL+E
Sbjct: 70  QMQDNLLLYSRAYWLTQSLIAWNVDHHQNGICYLLSSKDASLNISNSQIQGEDLKIKLQE 129

Query: 129 DTAGLPANVVEKFPHIQGYRAFKLPPNLDIKSLLKSQLVVAIYDSDEKCRDCTGLQLPGV 188
           D AGLPANVVEKFPHI+GY+AF LPP  DIKSLLKSQL V IYDSDEKCRDCTGLQLPGV
Sbjct: 130 DKAGLPANVVEKFPHIRGYKAFNLPPGSDIKSLLKSQLAVVIYDSDEKCRDCTGLQLPGV 189

Query: 189 LDELFSYNGPLGAHFSEEAVSLYLWAPTAQAVHAYIYRDPSGDDPIEIVPLEEEHGVWRT 248
           LDELFSYNGPLGA FSEEAVSLYLWAPTAQ+V AYIY+ PSGDDPIEIVPLEEEHGVWRT
Sbjct: 190 LDELFSYNGPLGALFSEEAVSLYLWAPTAQSVRAYIYKHPSGDDPIEIVPLEEEHGVWRT 249

Query: 249 EGPKCWEGXXXXXXXXXXHPSTLRFEKCYANDPYARGLSADGRRTFLLNIDSDELKPDGW 308
           +GPK WEG          HPST R EKCYANDPYARGLS+DGRRTFLLN+DS+ELKPD W
Sbjct: 250 KGPKSWEGCYYVYEVCVYHPSTSRVEKCYANDPYARGLSSDGRRTFLLNLDSNELKPDRW 309

Query: 309 DSLANEKPGLHSFSDISIYEMHIRDFSANDLSVQSEFRGGYLAFTLLESAGVLHLKKLSS 368
           ++LA+EKP LHSFSDISIYE+HIRDFSANDLSVQ EFRGGYLAFTL +SAGVLHLKKLSS
Sbjct: 310 ENLADEKPILHSFSDISIYELHIRDFSANDLSVQPEFRGGYLAFTLTDSAGVLHLKKLSS 369

Query: 369 AGVTHVHLLPTFQFAGVDDRKENWRFVDTSILESFPADSDQQQALITAIQNVDAYNWGYN 428
           AG+THVHLLPTFQFAGVDD+KENWR VDTS+LESFP DSDQQQALITAIQN D YNWGYN
Sbjct: 370 AGITHVHLLPTFQFAGVDDQKENWRNVDTSVLESFPPDSDQQQALITAIQNFDGYNWGYN 429

Query: 429 PVLWGVPKGSYASNPNGPYRIIEFRKMIQALNRTGLRIVLDTVYNHLQGSGPFDDHSVLD 488
           PVLWGVPKGSYASNPNGP R IEFRKM+QAL+  GLR+VLDTVYNHLQG+GPFD+HSVLD
Sbjct: 430 PVLWGVPKGSYASNPNGPNRTIEFRKMVQALSHIGLRVVLDTVYNHLQGNGPFDEHSVLD 489

Query: 489 KIVPGYYLRRNTDGFIENSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGFRFDLMGHI 548
           KIVPGYYLRRNTDGFIENSTCMNNTASEH MVERLILDDL+HWAVNYK+DGFRFDLMGHI
Sbjct: 490 KIVPGYYLRRNTDGFIENSTCMNNTASEHSMVERLILDDLLHWAVNYKVDGFRFDLMGHI 549

Query: 549 MKSTMVKAKNALHCLRKEKDGVDGSSIYIYGEGWDFGEVANNGRGINASQFNLSGTQIGS 608
           MKSTMVKAKNAL  L KEKDGVDGSSIYIYGEGWDFGEVA NGRGINASQFNL+GTQIGS
Sbjct: 550 MKSTMVKAKNALQRLTKEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNLAGTQIGS 609

Query: 609 FNDRIRDAILGGSPFGHPLQQGFVTGLLLQPNGHDHGTEANAKSMLATSMDHIQVGMAAN 668
           FNDRIRD++LGGSPFGHPLQQGFVTGLLLQPNGHDHGTEAN KSMLA SMDHIQ+GMA N
Sbjct: 610 FNDRIRDSVLGGSPFGHPLQQGFVTGLLLQPNGHDHGTEANMKSMLAASMDHIQIGMAGN 669

Query: 669 LKDFVLTNSKGEEVKGSEVLTYGGTPVAYASCPTDTINYVSAHDNETLFDIVSLKTPMDI 728
           LKDFVLTNS+GEEVKGSEVLTYGGTPVA+AS P +T+NYVSAHDNETLFDIVSLKTPMDI
Sbjct: 670 LKDFVLTNSEGEEVKGSEVLTYGGTPVAFASYPIETVNYVSAHDNETLFDIVSLKTPMDI 729

Query: 729 NVAERCRINHLATSIIALSQGIPFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYNSNNW 788
            VAERCRINHLATS+IALSQGIPFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYNSNNW
Sbjct: 730 GVAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYNSNNW 789

Query: 789 GVGLPPQEKNEKSWPLIKPRLADPSFKPQKINILAALDNFLNLLRIRYSSPLFRLRTANA 848
           GVGLPPQEKNEK W L+KPRLADPSF+PQ+I+ILA +DNFLNLLRIRYSS LFRLRTANA
Sbjct: 790 GVGLPPQEKNEKHWSLMKPRLADPSFRPQRIDILATMDNFLNLLRIRYSSALFRLRTANA 849

Query: 849 IQQRVCFHNTGPSLVDGVIVMSIEDGHEGFPGLPQLDPIYSFIVVVVNAGPEEVSFVSPS 908
           IQQRV FHNTGPSLV GVIVMSIEDGH+GFPGL QLDPIYSFIVVV NA P+EVSFVSPS
Sbjct: 850 IQQRVRFHNTGPSLVSGVIVMSIEDGHDGFPGLSQLDPIYSFIVVVFNASPQEVSFVSPS 909

Query: 909 LQSRSLQLHPILVTSSDELVKSSTYEASSGC 939
           LQSR+LQLHPI   SSDELVKSS YEASSGC
Sbjct: 910 LQSRNLQLHPIQEMSSDELVKSSKYEASSGC 940


>Medtr3g099500.1 | isoamylase-type starch debranching enzyme | HC |
           chr3:45613850-45627548 | 20130731
          Length = 777

 Score =  105 bits (263), Expect = 1e-22,   Method: Compositional matrix adjust.
 Identities = 138/507 (27%), Positives = 207/507 (40%), Gaps = 85/507 (16%)

Query: 280 DPYARGLSADGRR----------TFLLNIDSDELKPDGWDSLANEKPGLHSFSDISIYEM 329
           DPYA+ +  +GRR           FL   D D L P  W    N  P +    D+ IYEM
Sbjct: 200 DPYAKLI--EGRRYFGDISRKLSKFLGTYDFDSL-PFDWGENYN-LPNIAE-KDLVIYEM 254

Query: 330 HIRDFSANDLS-VQSEFRGGYLAFTLLESAGVLHLKKLSSAGVTHVHLLPTFQFAGVDDR 388
           ++R F+ ++ S + +  RG YL   ++E   + HL +L   G+  V LLP F+F      
Sbjct: 255 NVRAFTMDESSELDNNIRGSYLG--VIEK--IPHLLEL---GINAVELLPIFEF------ 301

Query: 389 KENWRFVDTSILESFPADSDQQQALITAIQNVDAYNWGYNPVLWGVPKGSYASNPNGPYR 448
                  D   L+  P   D    +I          WGY+ + +  P   YAS   GP  
Sbjct: 302 -------DEMELQRRPNPRDH---MINT--------WGYSTINFFAPMSRYASAGGGPAN 343

Query: 449 II-EFRKMIQALNRTGLRIVLDTVYNHLQGSGPFDDHSV----LDKIVPGYYLRRNTDGF 503
              E ++M++AL+  G+ ++LD VYNH   +   + ++     +D  V  YY+  +    
Sbjct: 344 ASQELKQMVKALHSAGIEVILDVVYNHTNEADDPNPYTTSFRGIDNKV--YYMLDDKGQL 401

Query: 504 IENSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGFRFDLMGHIMKSTMVKAKNALHCL 563
           +  S C N     H +V  LILD L HW   Y +DGFRFDL   + + T     NA   +
Sbjct: 402 LNFSGCGNTLNCNHPVVMDLILDSLRHWVTEYHVDGFRFDLASILCRGTDGSPLNAPPLI 461

Query: 564 RKEKDGVDGSSIYIYGEGWDFGEVANNGRGINASQFNLSGTQIGSFNDRIRDAILGGSPF 623
           R        S   I  E WD G +   G   N  ++       G + D IR  I G S  
Sbjct: 462 RAIAKDAVLSRCKIIAEPWDCGGLYLVGSFPNWDRW---AEWNGKYRDDIRKFIKGDS-- 516

Query: 624 GHPLQQGFVTGLLLQPNGHDHGTEANAKSMLATSMDHIQVGMAANLKDFVLTNSKGEEVK 683
                 G       + +G       N K      ++ +      +L+D V  N K  E  
Sbjct: 517 ------GMKGSFATRVSGSSDLYRVN-KRRPYHGINFVIAHDGFSLRDLVSYNFKHNEAN 569

Query: 684 GSEVLTYGGTPVAYASCPTDTINYVSAHDNETLFDIVSLKTPMDINVAERCRINHLATSI 743
           G      GG       C  D  ++    + ET  D  S++       + + +  HLA   
Sbjct: 570 GE-----GGND----GC-NDNFSWNCGFEGET--DDASIRALR----SRQMKNFHLA--- 610

Query: 744 IALSQGIPFFHSGDEILRSKSLDRDSY 770
           + +SQG P    GDE   ++  + +SY
Sbjct: 611 LMISQGTPMMLMGDEYGHTRYGNNNSY 637


>Medtr3g099500.2 | isoamylase-type starch debranching enzyme | HC |
           chr3:45613850-45627548 | 20130731
          Length = 794

 Score =  105 bits (263), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 137/517 (26%), Positives = 205/517 (39%), Gaps = 88/517 (17%)

Query: 280 DPYARGLSADGRR----------TFLLNIDSDELKPDGWDSLANEKPGLHSFSDISIYEM 329
           DPYA+ +  +GRR           FL   D D L P  W    N  P +    D+ IYEM
Sbjct: 200 DPYAKLI--EGRRYFGDISRKLSKFLGTYDFDSL-PFDWGENYN-LPNIAE-KDLVIYEM 254

Query: 330 HIRDFSANDLS-VQSEFRGGYL----------AFTLLESAGVLHLKKLSSAGVTHVHLLP 378
           ++R F+ ++ S + +  RG YL          +  +    GV  +  L   G+  V LLP
Sbjct: 255 NVRAFTMDESSELDNNIRGSYLGVIEKVTSVLSLGITVQYGVSMIPHLLELGINAVELLP 314

Query: 379 TFQFAGVDDRKENWRFVDTSILESFPADSDQQQALITAIQNVDAYNWGYNPVLWGVPKGS 438
            F+F             D   L+  P   D    +I          WGY+ + +  P   
Sbjct: 315 IFEF-------------DEMELQRRPNPRDH---MINT--------WGYSTINFFAPMSR 350

Query: 439 YASNPNGPYRII-EFRKMIQALNRTGLRIVLDTVYNHLQGSGPFDDHSV----LDKIVPG 493
           YAS   GP     E ++M++AL+  G+ ++LD VYNH   +   + ++     +D  V  
Sbjct: 351 YASAGGGPANASQELKQMVKALHSAGIEVILDVVYNHTNEADDPNPYTTSFRGIDNKV-- 408

Query: 494 YYLRRNTDGFIENSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGFRFDLMGHIMKSTM 553
           YY+  +    +  S C N     H +V  LILD L HW   Y +DGFRFDL   + + T 
Sbjct: 409 YYMLDDKGQLLNFSGCGNTLNCNHPVVMDLILDSLRHWVTEYHVDGFRFDLASILCRGTD 468

Query: 554 VKAKNALHCLRKEKDGVDGSSIYIYGEGWDFGEVANNGRGINASQFNLSGTQIGSFNDRI 613
               NA   +R        S   I  E WD G +   G   N  ++       G + D I
Sbjct: 469 GSPLNAPPLIRAIAKDAVLSRCKIIAEPWDCGGLYLVGSFPNWDRW---AEWNGKYRDDI 525

Query: 614 RDAILGGSPFGHPLQQGFVTGLLLQPNGHDHGTEANAKSMLATSMDHIQVGMAANLKDFV 673
           R  I G S        G       + +G       N K      ++ +      +L+D V
Sbjct: 526 RKFIKGDS--------GMKGSFATRVSGSSDLYRVN-KRRPYHGINFVIAHDGFSLRDLV 576

Query: 674 LTNSKGEEVKGSEVLTYGGTPVAYASCPTDTINYVSAHDNETLFDIVSLKTPMDINVAER 733
             N K  E  G      GG       C  D  ++    + ET  D  S++       + +
Sbjct: 577 SYNFKHNEANGE-----GGND----GC-NDNFSWNCGFEGET--DDASIRALR----SRQ 620

Query: 734 CRINHLATSIIALSQGIPFFHSGDEILRSKSLDRDSY 770
            +  HLA   + +SQG P    GDE   ++  + +SY
Sbjct: 621 MKNFHLA---LMISQGTPMMLMGDEYGHTRYGNNNSY 654


>Medtr8g101490.1 | isoamylase-type starch debranching enzyme | HC |
           chr8:42635829-42624990 | 20130731
          Length = 788

 Score = 85.1 bits (209), Expect = 3e-16,   Method: Compositional matrix adjust.
 Identities = 125/543 (23%), Positives = 202/543 (37%), Gaps = 123/543 (22%)

Query: 280 DPYARGLSADGRRTFLLNIDSDELKPDG--WDSLA------NEK-------PGLHSFSDI 324
           DPYA+ + + G        +  E+ PDG  W  +A      NE+       P  +   D+
Sbjct: 172 DPYAKAVISRG--------EFGEVGPDGNCWPQMAGMVPFDNEEFDWEGDLPLKYPQKDL 223

Query: 325 SIYEMHIRDFSANDLSVQSEFRGGYLAFTLLESAGVLHLKKLSSAGVTHVHLLPTFQFAG 384
            IYEMH+R F+ ++ S ++EF G YL         V  L  L   GV  + L+P  +F  
Sbjct: 224 IIYEMHVRGFTKHE-SSKAEFPGTYLGV-------VEKLDHLKELGVNCIELMPCHEFNE 275

Query: 385 VDDRKENWRFVDTSILESFPADSDQQQALITAIQNVDAYN-WGYNPVLWGVPKGSYAS-- 441
           ++    N                        AIQ     N WGY+ + +  P   Y+S  
Sbjct: 276 LEYYSYN------------------------AIQGDYRVNFWGYSTINYFSPMIRYSSAG 311

Query: 442 -NPNGPYRIIEFRKMIQALNRTGLRIVLDTVYNHL---QGSGPFDDHSVLDKIVPGYYLR 497
               G   I E + +I+  ++ G+ +++D V+NH       GP      +D  V  YY+ 
Sbjct: 312 IQNCGRDGINEMKLLIKEAHKRGIEVIMDVVFNHTAEGNEKGPIISFRGVDNSV--YYMV 369

Query: 498 RNTDGFIENSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGFRFDLMGHIMKSTMVKAK 557
                F   S C N     H +V + I+D L +W     +DGFRFDL   + +S+ +   
Sbjct: 370 APKGEFYNYSGCGNTFNCNHPVVRKFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSL--W 427

Query: 558 NALHCLRKEKDG--------------VDGSSI-------YIYGEGWDFGEVANNGRGINA 596
           N ++      +G              +D  SI        +  E WD G +   G     
Sbjct: 428 NGVNVFGAPIEGDFLTTGTPLSSPPLIDMISIDPILRGVKLIAEAWDAGGLYQVG---TF 484

Query: 597 SQFNLSGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLLLQPNGHDHGTEANAKSMLAT 656
             + +     G + D +R  I G   F       F   +   PN +  G +      L  
Sbjct: 485 PHWGIWSEWNGKYRDTVRQFIKGTDGFA----GAFAECICGSPNLYQGGRKPWNSINLVC 540

Query: 657 SMDHIQVGMAANLKDFVLTNSKGEEVKGSEVLTYGGTPVAYASCPTDTINYVSAHDNETL 716
           + D         L D V  N+K     G +                D  N+ S+ +    
Sbjct: 541 AHD------GFTLADLVTYNNKHNLPNGED--------------NNDGENHNSSWNCGEE 580

Query: 717 FDIVSLKTPMDINVAERCRINHLATSIIAL--SQGIPFFHSGDEILRSKSLDRDSYNSGD 774
            + VS          ++ R   +    ++L  SQG+P    GDE   +K  + ++Y   +
Sbjct: 581 GEFVSAS-------VKKMRKRQMRNFFLSLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDN 633

Query: 775 WFN 777
           + N
Sbjct: 634 YLN 636


>Medtr8g101490.2 | isoamylase-type starch debranching enzyme | HC |
           chr8:42635829-42624990 | 20130731
          Length = 660

 Score = 84.3 bits (207), Expect = 5e-16,   Method: Compositional matrix adjust.
 Identities = 78/294 (26%), Positives = 125/294 (42%), Gaps = 62/294 (21%)

Query: 280 DPYARGLSADGRRTFLLNIDSDELKPDG--WDSLA------NEK-------PGLHSFSDI 324
           DPYA+ + + G        +  E+ PDG  W  +A      NE+       P  +   D+
Sbjct: 172 DPYAKAVISRG--------EFGEVGPDGNCWPQMAGMVPFDNEEFDWEGDLPLKYPQKDL 223

Query: 325 SIYEMHIRDFSANDLSVQSEFRGGYLAFTLLESAGVLHLKKLSSAGVTHVHLLPTFQFAG 384
            IYEMH+R F+ ++ S ++EF G YL         V  L  L   GV  + L+P  +F  
Sbjct: 224 IIYEMHVRGFTKHE-SSKAEFPGTYLGV-------VEKLDHLKELGVNCIELMPCHEF-- 273

Query: 385 VDDRKENWRFVDTSILESFPADSDQQQALITAIQNVDAYNWGYNPVLWGVPKGSYAS--- 441
                        + LE +  ++ Q    +          WGY+ + +  P   Y+S   
Sbjct: 274 -------------NELEYYSYNAIQGDYRVNF--------WGYSTINYFSPMIRYSSAGI 312

Query: 442 NPNGPYRIIEFRKMIQALNRTGLRIVLDTVYNHL---QGSGPFDDHSVLDKIVPGYYLRR 498
              G   I E + +I+  ++ G+ +++D V+NH       GP      +D  V  YY+  
Sbjct: 313 QNCGRDGINEMKLLIKEAHKRGIEVIMDVVFNHTAEGNEKGPIISFRGVDNSV--YYMVA 370

Query: 499 NTDGFIENSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGFRFDLMGHIMKST 552
               F   S C N     H +V + I+D L +W     +DGFRFDL   + +S+
Sbjct: 371 PKGEFYNYSGCGNTFNCNHPVVRKFIVDCLRYWVTEMHVDGFRFDLASIMTRSS 424