Miyakogusa Predicted Gene

Lj5g3v0709800.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v0709800.2 Non Characterized Hit- tr|I1LZF1|I1LZF1_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.27919 PE,86.72,0,C-14
STEROL REDUCTASE,NULL; STEROL REDUCTASE/LAMIN B RECEPTOR,NULL;
ERG4_ERG24,Ergosterol biosynthes,CUFF.53830.2
         (369 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g061240.1 | sterol C-14 reductase | HC | chr1:26778541-267...   519   e-147
Medtr4g005450.1 | 7-dehydrocholesterol reductase-like protein | ...   111   1e-24
Medtr4g094512.3 | 7-dehydrocholesterol reductase-like protein | ...   100   3e-21
Medtr4g094512.5 | 7-dehydrocholesterol reductase-like protein | ...   100   3e-21
Medtr4g094512.1 | 7-dehydrocholesterol reductase-like protein | ...   100   3e-21
Medtr4g094512.4 | 7-dehydrocholesterol reductase-like protein | ...   100   3e-21
Medtr4g094512.2 | 7-dehydrocholesterol reductase-like protein | ...   100   3e-21
Medtr4g094510.1 | 7-dehydrocholesterol reductase-like protein | ...    83   3e-16

>Medtr1g061240.1 | sterol C-14 reductase | HC |
           chr1:26778541-26772409 | 20130731
          Length = 369

 Score =  519 bits (1337), Expect = e-147,   Method: Compositional matrix adjust.
 Identities = 263/369 (71%), Positives = 278/369 (75%)

Query: 1   MDLGCPVHGLIPSWNSXXXXXXXXXXXXXXXSVVPGKLIAGVVLPDKSRLRYRCNGXXXX 60
           MDL   +H LIPSWNS               S++PGKL+ GVVL D +RL YRCNG    
Sbjct: 1   MDLDFLIHALIPSWNSVALLAGFFTYLAIVGSILPGKLVPGVVLSDSTRLHYRCNGLLSL 60

Query: 61  XXXXXXXGIGAKMGFVSPTAISERGLELLSTTFIFSFLVALILYFXXXXXXXXXXXXXXX 120
                   I AKM FVS TAI++RGLELLSTTFIFSFLVALILYF               
Sbjct: 61  VLLVGLLWISAKMEFVSLTAIADRGLELLSTTFIFSFLVALILYFSGCKSRSKGSSLKPH 120

Query: 121 XXGNLIHDWWFGIQLNPQVMGIDLKFFFVRAGMMGWLLINLSVLAKSIQDDTLSQSMILF 180
             G+LIHDWWFGIQLNPQ MGIDLKFFFVRAGMMGWLLINLSVLAKSIQD TLS+SMILF
Sbjct: 121 ISGDLIHDWWFGIQLNPQFMGIDLKFFFVRAGMMGWLLINLSVLAKSIQDGTLSKSMILF 180

Query: 181 QLFCGIYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLRNKVELT 240
           QLF  +YILDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLRNKVELT
Sbjct: 181 QLFNALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLRNKVELT 240

Query: 241 TAAIVANCFVFLIGYMVFRGANKQKHEFKKNPKAPIWGKPPIVIGGKLLASGYWGVARHC 300
            AA+VANCFVFLIGYMVFRGANKQKH+FKKNPKAPIWG PP VIGGKLLASGYWGVARHC
Sbjct: 241 NAAVVANCFVFLIGYMVFRGANKQKHDFKKNPKAPIWGNPPKVIGGKLLASGYWGVARHC 300

Query: 301 NYXXXXXXXXXXXXPCGISSXXXXXXXXXXXXXXXWRERRDEARCAEKYKEIWAEYRRTV 360
           NY            PCGISS               WRERRDEARCAEKY++IW EYR+ V
Sbjct: 301 NYLGDLLLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEARCAEKYRDIWLEYRKLV 360

Query: 361 PWRILPYVY 369
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369


>Medtr4g005450.1 | 7-dehydrocholesterol reductase-like protein | HC
           | chr4:262823-259097 | 20130731
          Length = 277

 Score =  111 bits (278), Expect = 1e-24,   Method: Compositional matrix adjust.
 Identities = 77/266 (28%), Positives = 130/266 (48%), Gaps = 22/266 (8%)

Query: 123 GNLIHDWWFGIQLNPQVMG-IDLKFFF-VRAGMMGWLLINLSVLAKSIQDD-TLSQSMIL 179
           GN+I D+++G++L P++    D+K F   R GMM W ++ L+   K  +++  ++ SM++
Sbjct: 15  GNIIIDFYWGMELYPRIGKYFDIKVFTNCRFGMMSWAVLALTYCIKQYEENGKVADSMLV 74

Query: 180 FQLFCGIYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLRNKVEL 239
                 +Y+  +F  E    +T DI  +R GF + +G LVW+P  ++  G +L+ + V L
Sbjct: 75  NTTLMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNL 134

Query: 240 TTAAIVANCFVFLIGYM---VFRGANKQKHEFKK-NPKAPIWGKPPIVIGGK-------- 287
            T  ++   F+ + G M   +    ++Q  EF++ N K+ +WGK P  I           
Sbjct: 135 GTQLVL---FILVAGIMCIYINYDCDRQSQEFRRTNGKSLVWGKAPSKIEASYTTASGET 191

Query: 288 ----LLASGYWGVARHCNYXXXXXXXXXXXXPCGISSXXXXXXXXXXXXXXXWRERRDEA 343
               LL SG+WG+ARH +Y            P   +                 R +RD+ 
Sbjct: 192 KRSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLIILLFDRAKRDDD 251

Query: 344 RCAEKYKEIWAEYRRTVPWRILPYVY 369
           RC  KY + W  Y   VP+RI+P +Y
Sbjct: 252 RCRSKYGKYWKLYCDRVPYRIIPGIY 277


>Medtr4g094512.3 | 7-dehydrocholesterol reductase-like protein | HC
           | chr4:38115312-38122132 | 20130731
          Length = 396

 Score =  100 bits (249), Expect = 3e-21,   Method: Compositional matrix adjust.
 Identities = 83/340 (24%), Positives = 141/340 (41%), Gaps = 35/340 (10%)

Query: 33  VVPGKLIAGVVLPDKSRLRYRCNGXXXXXXXXXXXGIGAKMGFVSPTAISERGLELLSTT 92
           ++PG+ + G + P  +R  Y+ NG               + G  +PT + +   E+ ST 
Sbjct: 89  LLPGETVYGPISPTGNRPFYKANGVAAYLVTLVTYVALWRFGIFNPTIVYDHLGEIYSTL 148

Query: 93  FIFSFLVALILYFXXXXXXXXXXXXXXXXXGNLIHDWWFGIQLNPQV-MGIDLKFFF-VR 150
              SF+  + LY                  GN+I D+++G++L P++    D+K F   R
Sbjct: 149 SFGSFIFCIFLYIKGHLAPSSTDSGSS---GNIIIDFYWGMELYPRIGKYFDIKVFTNCR 205

Query: 151 AGMMGWLLINLSVLAKSIQDD-TLSQSMILFQLFCGIYILDYFVHEEYMTSTWDIIAERL 209
            GMM W ++ L+   K  +++  ++ SM++      +Y+  +F  E    +T DI  +R 
Sbjct: 206 FGMMSWAVLALTYCIKQYEENGKVADSMLVNTTLMLVYVTKFFWWEAGYWNTMDIAHDRA 265

Query: 210 GFMLVFGDLVWIPFTFSIQGWWLLRNKVELTTAAIVANCFVFLIGYMVFRGANKQKHEFK 269
           GF + +G LVW+P  ++  G +L+ + V L T    +        Y    G  K+     
Sbjct: 266 GFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQIEAS--------YTTSSGETKRS---- 313

Query: 270 KNPKAPIWGKPPIVIGGKLLASGYWGVARHCNYXXXXXXXXXXXXPCGISSXXXXXXXXX 329
                             LL SG+W +ARH +Y            P   +          
Sbjct: 314 -----------------LLLTSGWWRLARHFHYVPEILAAFFWTVPALFNHFLPYLYVIF 356

Query: 330 XXXXXXWRERRDEARCAEKYKEIWAEYRRTVPWRILPYVY 369
                  R +RD+ RC  KY + W  Y   V +RI+P +Y
Sbjct: 357 LIILLFDRAKRDDDRCRSKYGKYWKLYCDRVAYRIIPGIY 396


>Medtr4g094512.5 | 7-dehydrocholesterol reductase-like protein | HC
           | chr4:38115286-38122132 | 20130731
          Length = 396

 Score =  100 bits (249), Expect = 3e-21,   Method: Compositional matrix adjust.
 Identities = 83/340 (24%), Positives = 141/340 (41%), Gaps = 35/340 (10%)

Query: 33  VVPGKLIAGVVLPDKSRLRYRCNGXXXXXXXXXXXGIGAKMGFVSPTAISERGLELLSTT 92
           ++PG+ + G + P  +R  Y+ NG               + G  +PT + +   E+ ST 
Sbjct: 89  LLPGETVYGPISPTGNRPFYKANGVAAYLVTLVTYVALWRFGIFNPTIVYDHLGEIYSTL 148

Query: 93  FIFSFLVALILYFXXXXXXXXXXXXXXXXXGNLIHDWWFGIQLNPQV-MGIDLKFFF-VR 150
              SF+  + LY                  GN+I D+++G++L P++    D+K F   R
Sbjct: 149 SFGSFIFCIFLYIKGHLAPSSTDSGSS---GNIIIDFYWGMELYPRIGKYFDIKVFTNCR 205

Query: 151 AGMMGWLLINLSVLAKSIQDD-TLSQSMILFQLFCGIYILDYFVHEEYMTSTWDIIAERL 209
            GMM W ++ L+   K  +++  ++ SM++      +Y+  +F  E    +T DI  +R 
Sbjct: 206 FGMMSWAVLALTYCIKQYEENGKVADSMLVNTTLMLVYVTKFFWWEAGYWNTMDIAHDRA 265

Query: 210 GFMLVFGDLVWIPFTFSIQGWWLLRNKVELTTAAIVANCFVFLIGYMVFRGANKQKHEFK 269
           GF + +G LVW+P  ++  G +L+ + V L T    +        Y    G  K+     
Sbjct: 266 GFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQIEAS--------YTTSSGETKRS---- 313

Query: 270 KNPKAPIWGKPPIVIGGKLLASGYWGVARHCNYXXXXXXXXXXXXPCGISSXXXXXXXXX 329
                             LL SG+W +ARH +Y            P   +          
Sbjct: 314 -----------------LLLTSGWWRLARHFHYVPEILAAFFWTVPALFNHFLPYLYVIF 356

Query: 330 XXXXXXWRERRDEARCAEKYKEIWAEYRRTVPWRILPYVY 369
                  R +RD+ RC  KY + W  Y   V +RI+P +Y
Sbjct: 357 LIILLFDRAKRDDDRCRSKYGKYWKLYCDRVAYRIIPGIY 396


>Medtr4g094512.1 | 7-dehydrocholesterol reductase-like protein | HC
           | chr4:38115314-38122132 | 20130731
          Length = 396

 Score =  100 bits (249), Expect = 3e-21,   Method: Compositional matrix adjust.
 Identities = 83/340 (24%), Positives = 141/340 (41%), Gaps = 35/340 (10%)

Query: 33  VVPGKLIAGVVLPDKSRLRYRCNGXXXXXXXXXXXGIGAKMGFVSPTAISERGLELLSTT 92
           ++PG+ + G + P  +R  Y+ NG               + G  +PT + +   E+ ST 
Sbjct: 89  LLPGETVYGPISPTGNRPFYKANGVAAYLVTLVTYVALWRFGIFNPTIVYDHLGEIYSTL 148

Query: 93  FIFSFLVALILYFXXXXXXXXXXXXXXXXXGNLIHDWWFGIQLNPQV-MGIDLKFFF-VR 150
              SF+  + LY                  GN+I D+++G++L P++    D+K F   R
Sbjct: 149 SFGSFIFCIFLYIKGHLAPSSTDSGSS---GNIIIDFYWGMELYPRIGKYFDIKVFTNCR 205

Query: 151 AGMMGWLLINLSVLAKSIQDD-TLSQSMILFQLFCGIYILDYFVHEEYMTSTWDIIAERL 209
            GMM W ++ L+   K  +++  ++ SM++      +Y+  +F  E    +T DI  +R 
Sbjct: 206 FGMMSWAVLALTYCIKQYEENGKVADSMLVNTTLMLVYVTKFFWWEAGYWNTMDIAHDRA 265

Query: 210 GFMLVFGDLVWIPFTFSIQGWWLLRNKVELTTAAIVANCFVFLIGYMVFRGANKQKHEFK 269
           GF + +G LVW+P  ++  G +L+ + V L T    +        Y    G  K+     
Sbjct: 266 GFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQIEAS--------YTTSSGETKRS---- 313

Query: 270 KNPKAPIWGKPPIVIGGKLLASGYWGVARHCNYXXXXXXXXXXXXPCGISSXXXXXXXXX 329
                             LL SG+W +ARH +Y            P   +          
Sbjct: 314 -----------------LLLTSGWWRLARHFHYVPEILAAFFWTVPALFNHFLPYLYVIF 356

Query: 330 XXXXXXWRERRDEARCAEKYKEIWAEYRRTVPWRILPYVY 369
                  R +RD+ RC  KY + W  Y   V +RI+P +Y
Sbjct: 357 LIILLFDRAKRDDDRCRSKYGKYWKLYCDRVAYRIIPGIY 396


>Medtr4g094512.4 | 7-dehydrocholesterol reductase-like protein | HC
           | chr4:38115295-38122132 | 20130731
          Length = 396

 Score =  100 bits (249), Expect = 3e-21,   Method: Compositional matrix adjust.
 Identities = 83/340 (24%), Positives = 141/340 (41%), Gaps = 35/340 (10%)

Query: 33  VVPGKLIAGVVLPDKSRLRYRCNGXXXXXXXXXXXGIGAKMGFVSPTAISERGLELLSTT 92
           ++PG+ + G + P  +R  Y+ NG               + G  +PT + +   E+ ST 
Sbjct: 89  LLPGETVYGPISPTGNRPFYKANGVAAYLVTLVTYVALWRFGIFNPTIVYDHLGEIYSTL 148

Query: 93  FIFSFLVALILYFXXXXXXXXXXXXXXXXXGNLIHDWWFGIQLNPQV-MGIDLKFFF-VR 150
              SF+  + LY                  GN+I D+++G++L P++    D+K F   R
Sbjct: 149 SFGSFIFCIFLYIKGHLAPSSTDSGSS---GNIIIDFYWGMELYPRIGKYFDIKVFTNCR 205

Query: 151 AGMMGWLLINLSVLAKSIQDD-TLSQSMILFQLFCGIYILDYFVHEEYMTSTWDIIAERL 209
            GMM W ++ L+   K  +++  ++ SM++      +Y+  +F  E    +T DI  +R 
Sbjct: 206 FGMMSWAVLALTYCIKQYEENGKVADSMLVNTTLMLVYVTKFFWWEAGYWNTMDIAHDRA 265

Query: 210 GFMLVFGDLVWIPFTFSIQGWWLLRNKVELTTAAIVANCFVFLIGYMVFRGANKQKHEFK 269
           GF + +G LVW+P  ++  G +L+ + V L T    +        Y    G  K+     
Sbjct: 266 GFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQIEAS--------YTTSSGETKRS---- 313

Query: 270 KNPKAPIWGKPPIVIGGKLLASGYWGVARHCNYXXXXXXXXXXXXPCGISSXXXXXXXXX 329
                             LL SG+W +ARH +Y            P   +          
Sbjct: 314 -----------------LLLTSGWWRLARHFHYVPEILAAFFWTVPALFNHFLPYLYVIF 356

Query: 330 XXXXXXWRERRDEARCAEKYKEIWAEYRRTVPWRILPYVY 369
                  R +RD+ RC  KY + W  Y   V +RI+P +Y
Sbjct: 357 LIILLFDRAKRDDDRCRSKYGKYWKLYCDRVAYRIIPGIY 396


>Medtr4g094512.2 | 7-dehydrocholesterol reductase-like protein | HC
           | chr4:38115312-38122132 | 20130731
          Length = 396

 Score =  100 bits (249), Expect = 3e-21,   Method: Compositional matrix adjust.
 Identities = 83/340 (24%), Positives = 141/340 (41%), Gaps = 35/340 (10%)

Query: 33  VVPGKLIAGVVLPDKSRLRYRCNGXXXXXXXXXXXGIGAKMGFVSPTAISERGLELLSTT 92
           ++PG+ + G + P  +R  Y+ NG               + G  +PT + +   E+ ST 
Sbjct: 89  LLPGETVYGPISPTGNRPFYKANGVAAYLVTLVTYVALWRFGIFNPTIVYDHLGEIYSTL 148

Query: 93  FIFSFLVALILYFXXXXXXXXXXXXXXXXXGNLIHDWWFGIQLNPQV-MGIDLKFFF-VR 150
              SF+  + LY                  GN+I D+++G++L P++    D+K F   R
Sbjct: 149 SFGSFIFCIFLYIKGHLAPSSTDSGSS---GNIIIDFYWGMELYPRIGKYFDIKVFTNCR 205

Query: 151 AGMMGWLLINLSVLAKSIQDD-TLSQSMILFQLFCGIYILDYFVHEEYMTSTWDIIAERL 209
            GMM W ++ L+   K  +++  ++ SM++      +Y+  +F  E    +T DI  +R 
Sbjct: 206 FGMMSWAVLALTYCIKQYEENGKVADSMLVNTTLMLVYVTKFFWWEAGYWNTMDIAHDRA 265

Query: 210 GFMLVFGDLVWIPFTFSIQGWWLLRNKVELTTAAIVANCFVFLIGYMVFRGANKQKHEFK 269
           GF + +G LVW+P  ++  G +L+ + V L T    +        Y    G  K+     
Sbjct: 266 GFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQIEAS--------YTTSSGETKRS---- 313

Query: 270 KNPKAPIWGKPPIVIGGKLLASGYWGVARHCNYXXXXXXXXXXXXPCGISSXXXXXXXXX 329
                             LL SG+W +ARH +Y            P   +          
Sbjct: 314 -----------------LLLTSGWWRLARHFHYVPEILAAFFWTVPALFNHFLPYLYVIF 356

Query: 330 XXXXXXWRERRDEARCAEKYKEIWAEYRRTVPWRILPYVY 369
                  R +RD+ RC  KY + W  Y   V +RI+P +Y
Sbjct: 357 LIILLFDRAKRDDDRCRSKYGKYWKLYCDRVAYRIIPGIY 396


>Medtr4g094510.1 | 7-dehydrocholesterol reductase-like protein | HC
           | chr4:38108650-38112795 | 20130731
          Length = 239

 Score = 83.2 bits (204), Expect = 3e-16,   Method: Compositional matrix adjust.
 Identities = 65/250 (26%), Positives = 109/250 (43%), Gaps = 32/250 (12%)

Query: 123 GNLIHDWWFGIQLNPQV-MGIDLKFFF-VRAGMMGWLLINLSVLAKSIQDD-TLSQSMIL 179
           GN+I D+++G++L P++    D+K F   R GMM W ++ L+   K  +++  ++ SM++
Sbjct: 19  GNIIIDFYWGMELYPRIGKYFDIKVFTNCRFGMMSWAVLALTYCIKQYEENGKVADSMLV 78

Query: 180 FQLFCGIYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLRNKVEL 239
                 +Y+  +F  E    +T DI  +R GF + +G LVW+P  ++  G +L+ + V L
Sbjct: 79  NTTLMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNL 138

Query: 240 TTAAIVANCFVFLIGYMVFRGANKQKHEFKKNPKAPIWGKPPIVIGGKLLASGYWGVARH 299
            T    +        Y    G  K+                       LL SG+W +ARH
Sbjct: 139 GTQIEAS--------YTTSSGETKRS---------------------LLLTSGWWRLARH 169

Query: 300 CNYXXXXXXXXXXXXPCGISSXXXXXXXXXXXXXXXWRERRDEARCAEKYKEIWAEYRRT 359
            +Y            P   +                 R +RD+ RC  KY + W  Y   
Sbjct: 170 FHYVPEILAAFFWTVPALFNHFLPYLYVIFLIILLFDRAKRDDDRCRSKYGKYWKLYCDR 229

Query: 360 VPWRILPYVY 369
           V +RI+P +Y
Sbjct: 230 VAYRIIPGIY 239