Miyakogusa Predicted Gene

Lj5g3v0670650.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v0670650.1 tr|Q9STA5|Q9STA5_MEDTR Nodulin 6 OS=Medicago
truncatula GN=MtN6 PE=4 SV=1,73.11,0,no description,NULL; seg,NULL;
Amidohydro_2,Amidohydrolase 2; NODULIN / GLUTAMATE-AMMONIA
LIGASE-LIK,CUFF.53734.1
         (420 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g062710.1 | nodulin/glutamate-ammonia ligase-like protein ...   596   e-170
Medtr1g062620.1 | glutamate-ammonia ligase-like protein | HC | c...   555   e-158
Medtr1g062650.1 | glutamate-ammonia ligase-like protein | HC | c...   553   e-157
Medtr1g062620.3 | glutamate-ammonia ligase-like protein | HC | c...   486   e-137
Medtr1g062620.2 | glutamate-ammonia ligase-like protein | HC | c...   417   e-117
Medtr1g062620.4 | glutamate-ammonia ligase-like protein | HC | c...   335   7e-92

>Medtr1g062710.1 | nodulin/glutamate-ammonia ligase-like protein |
           HC | chr1:27448210-27451293 | 20130731
          Length = 423

 Score =  596 bits (1537), Expect = e-170,   Method: Compositional matrix adjust.
 Identities = 281/383 (73%), Positives = 329/383 (85%), Gaps = 2/383 (0%)

Query: 37  MDFNELRKAVEEVELVDGHAHNLVEVDSNFPFIHAFSLAHGDAVSFSNSSIAFKRNLRDV 96
           MDF+ LRK +E VELVDGHAHNLV VDSNF  IHAFSLAHGDAV+ +  S++FKRNLRDV
Sbjct: 42  MDFSVLRKTIESVELVDGHAHNLVAVDSNFSLIHAFSLAHGDAVASTQYSLSFKRNLRDV 101

Query: 97  AELYGTELSLEGVEEYRRVNGLHSISSTCFKAGRFSAILFDDGIGMDKVQDIEWHKSFIP 156
           AELYG E +LE VEEYRR++GL S+SSTCFKA R S ++FDDGI +DK+ D EWH+SF P
Sbjct: 102 AELYGCEPTLEAVEEYRRISGLESVSSTCFKAARISTVIFDDGIHLDKILDTEWHRSFTP 161

Query: 157 KVGRILRVEKLAEKILNEGLQGGSSWTLASFIGAFSSQLNSVVGEIYSLKSVVAYYAGLQ 216
            V R++RVE+LAE ILNEGL  G+SWTL SF  AF S+L SV  EI  LKS+ AYY GL 
Sbjct: 162 HVARLVRVERLAENILNEGLPNGTSWTLDSFTKAFLSKLESVADEIVGLKSIAAYYFGLD 221

Query: 217 INTNVTEEDAEEGLRQVLSAQMPIMITNKNLVDYLFLISLEFAQSHDLPMQIHTGFGDGG 276
           INT VT+++AEEGL+QVL+   P+++ NKNLVDY+FL+SLEF+QSHDLPMQ+HTGFGD  
Sbjct: 222 INTKVTKKEAEEGLQQVLAVFKPVLVANKNLVDYIFLLSLEFSQSHDLPMQLHTGFGDR- 280

Query: 277 YGLDLRLTNPLNLYNVLEDKRFSKSRIVLLHASYPFSKEASYLASVYSQVYLDFGLVIPK 336
            GL+LR++NPL L+NVLEDKRF+K RIVLLH SYPFSKEASYLAS+YSQVYLDFGL IPK
Sbjct: 281 -GLNLRMSNPLYLHNVLEDKRFAKCRIVLLHTSYPFSKEASYLASLYSQVYLDFGLAIPK 339

Query: 337 LSKHGMVTSVKGLLEQAPMSKVMFSTDSYAFPELFYLGAKNAREVVFTVLRDACIDGELS 396
           LS HGMV++VK LLEQAP++KVMFSTD+YAFPELFYLGAKNAREVVFTVLRD+CIDG+L+
Sbjct: 340 LSVHGMVSAVKDLLEQAPLNKVMFSTDAYAFPELFYLGAKNAREVVFTVLRDSCIDGDLT 399

Query: 397 VPEAVEAAKDLFARNAIQFYKIT 419
           VPEAVE AKDLFARN+I FY IT
Sbjct: 400 VPEAVEVAKDLFARNSINFYNIT 422


>Medtr1g062620.1 | glutamate-ammonia ligase-like protein | HC |
           chr1:27400992-27408624 | 20130731
          Length = 838

 Score =  555 bits (1431), Expect = e-158,   Method: Compositional matrix adjust.
 Identities = 269/385 (69%), Positives = 321/385 (83%), Gaps = 3/385 (0%)

Query: 37  MDFNELRKAVEEVELVDGHAHNLVEVDSNFPFIHAFSLAHGDAVSFSNSSIAFKRNLRDV 96
           MD +ELRKAVEEVELVDGHAHN+V + SN PFIHAFS A GDA++ S  S++FKRNLRD+
Sbjct: 1   MDLSELRKAVEEVELVDGHAHNIVSLQSNLPFIHAFSEAQGDALASSQHSLSFKRNLRDL 60

Query: 97  AELYGTELSLEGVEEYRRVNGLHSISSTCFKAGRFSAILFDDGIGMDKVQDIEWHKSFIP 156
           AELYG ELSL+GVEE+R+V+GL    STCFKA R SAIL DDG  +DK+ DIEWHKSF+P
Sbjct: 61  AELYGCELSLQGVEEHRKVSGLELSCSTCFKAARISAILMDDGFELDKMHDIEWHKSFVP 120

Query: 157 KVGRILRVEKLAEKILNEGLQGGSSWTLASFIGAFSSQLN-SVVGEIYSLKSVVAYYAGL 215
            VGRILR+EK+AE+IL++ L  GS WTL SF  AF S ++ +V GEIY LKS+ AY +GL
Sbjct: 121 LVGRILRIEKVAEEILDQDLPDGSCWTLDSFTKAFVSNISLTVAGEIYGLKSIAAYRSGL 180

Query: 216 QINTNVTEEDAEEGLRQVLSAQMPIMITNKNLVDYLFLISLEFAQSHDLPMQIHTGFGDG 275
           +INTNVT +DAEEGL QVL A+ P+ I NKNL+DY+FL SL+ AQS+DLPMQIHTGFGD 
Sbjct: 181 EINTNVTNQDAEEGLGQVLLARKPVRIANKNLIDYIFLQSLDVAQSYDLPMQIHTGFGDK 240

Query: 276 GYGLDLRLTNPLNLYNVLEDKRFSKSRIVLLHASYPFSKEASYLASVYSQVYLDFGLVIP 335
              LD+RL NPL+L +V EDKR+SKSRIVLLHASYPFS+EASYLASVY QVYLDFGL IP
Sbjct: 241 --DLDMRLANPLHLRSVFEDKRYSKSRIVLLHASYPFSREASYLASVYPQVYLDFGLAIP 298

Query: 336 KLSKHGMVTSVKGLLEQAPMSKVMFSTDSYAFPELFYLGAKNAREVVFTVLRDACIDGEL 395
           KLS HGM++S+K LLE AP++KVMFSTD   FPE FYLGAK +REVVF+VLRD+C+DG+L
Sbjct: 299 KLSVHGMISSLKELLELAPINKVMFSTDGCIFPETFYLGAKKSREVVFSVLRDSCLDGDL 358

Query: 396 SVPEAVEAAKDLFARNAIQFYKITI 420
           +VPEAVEAAK + ARN+I FYKI +
Sbjct: 359 TVPEAVEAAKGILARNSIHFYKINL 383


>Medtr1g062650.1 | glutamate-ammonia ligase-like protein | HC |
           chr1:27423091-27429047 | 20130731
          Length = 844

 Score =  553 bits (1425), Expect = e-157,   Method: Compositional matrix adjust.
 Identities = 268/385 (69%), Positives = 321/385 (83%), Gaps = 3/385 (0%)

Query: 37  MDFNELRKAVEEVELVDGHAHNLVEVDSNFPFIHAFSLAHGDAVSFSNSSIAFKRNLRDV 96
           MD +ELRKAVEEVELVDGHAHN+V + SN PFIH+FS AHGDA++ S  S++FKRNLRD+
Sbjct: 1   MDLSELRKAVEEVELVDGHAHNIVSLQSNLPFIHSFSEAHGDALASSQHSLSFKRNLRDL 60

Query: 97  AELYGTELSLEGVEEYRRVNGLHSISSTCFKAGRFSAILFDDGIGMDKVQDIEWHKSFIP 156
           AELYG ELSL+GVEE+R+V+GL    STCFKA R SAIL DDG+ +DK+ DIEWHKSFIP
Sbjct: 61  AELYGCELSLQGVEEHRKVSGLELSCSTCFKAARISAILMDDGLELDKMYDIEWHKSFIP 120

Query: 157 KVGRILRVEKLAEKILNEGLQGGSSWTLASFIGAF-SSQLNSVVGEIYSLKSVVAYYAGL 215
            VGRILR+E++AEKIL++ L  GS WTL S   AF S+   +   EIY LKS+ AY +GL
Sbjct: 121 LVGRILRIERVAEKILDQDLPDGSCWTLDSLTEAFLSNDTLTAAAEIYGLKSIAAYRSGL 180

Query: 216 QINTNVTEEDAEEGLRQVLSAQMPIMITNKNLVDYLFLISLEFAQSHDLPMQIHTGFGDG 275
           +INTNVTE+DAEEGLRQVL +  PI I NKNL+DY+FL SLE AQS+DLPMQIHTGF D 
Sbjct: 181 EINTNVTEKDAEEGLRQVLLSGKPIRIANKNLIDYIFLQSLEVAQSYDLPMQIHTGFRDK 240

Query: 276 GYGLDLRLTNPLNLYNVLEDKRFSKSRIVLLHASYPFSKEASYLASVYSQVYLDFGLVIP 335
              LD+RL NPL+L ++ EDK++SKSRIVLLHASYPFSKEASYLASVY QVYLDFGL IP
Sbjct: 241 --DLDMRLANPLHLRSIFEDKKYSKSRIVLLHASYPFSKEASYLASVYPQVYLDFGLAIP 298

Query: 336 KLSKHGMVTSVKGLLEQAPMSKVMFSTDSYAFPELFYLGAKNAREVVFTVLRDACIDGEL 395
           KLS HGM++S+K +LE AP++KVMFSTD YAFPE FYLGAK +REVVF+VLRD+C+DG+L
Sbjct: 299 KLSVHGMISSLKDILELAPINKVMFSTDGYAFPESFYLGAKKSREVVFSVLRDSCLDGDL 358

Query: 396 SVPEAVEAAKDLFARNAIQFYKITI 420
           +V EAVEA+KD+ ARN+I FYKI +
Sbjct: 359 TVTEAVEASKDILARNSIHFYKINL 383


>Medtr1g062620.3 | glutamate-ammonia ligase-like protein | HC |
           chr1:27400918-27408864 | 20130731
          Length = 790

 Score =  486 bits (1252), Expect = e-137,   Method: Compositional matrix adjust.
 Identities = 234/334 (70%), Positives = 278/334 (83%), Gaps = 2/334 (0%)

Query: 87  IAFKRNLRDVAELYGTELSLEGVEEYRRVNGLHSISSTCFKAGRFSAILFDDGIGMDKVQ 146
           +  KRNLRD+AELYG ELSL+GVEE+R+V+GL    STCFKA R SAIL DDG  +DK+ 
Sbjct: 4   LKLKRNLRDLAELYGCELSLQGVEEHRKVSGLELSCSTCFKAARISAILMDDGFELDKMH 63

Query: 147 DIEWHKSFIPKVGRILRVEKLAEKILNEGLQGGSSWTLASFIGAFSSQLNSVVGEIYSLK 206
           DIEWHKSF+P VGRILR+EK+AE+IL++ L  GS WTL SF  AF S+L SV GEIY LK
Sbjct: 64  DIEWHKSFVPLVGRILRIEKVAEEILDQDLPDGSCWTLDSFTKAFVSKLKSVAGEIYGLK 123

Query: 207 SVVAYYAGLQINTNVTEEDAEEGLRQVLSAQMPIMITNKNLVDYLFLISLEFAQSHDLPM 266
           S+ AY +GL+INTNVT +DAEEGL QVL A+ P+ I NKNL+DY+FL SL+ AQS+DLPM
Sbjct: 124 SIAAYRSGLEINTNVTNQDAEEGLGQVLLARKPVRIANKNLIDYIFLQSLDVAQSYDLPM 183

Query: 267 QIHTGFGDGGYGLDLRLTNPLNLYNVLEDKRFSKSRIVLLHASYPFSKEASYLASVYSQV 326
           QIHTGFGD    LD+RL NPL+L +V EDKR+SKSRIVLLHASYPFS+EASYLASVY QV
Sbjct: 184 QIHTGFGDK--DLDMRLANPLHLRSVFEDKRYSKSRIVLLHASYPFSREASYLASVYPQV 241

Query: 327 YLDFGLVIPKLSKHGMVTSVKGLLEQAPMSKVMFSTDSYAFPELFYLGAKNAREVVFTVL 386
           YLDFGL IPKLS HGM++S+K LLE AP++KVMFSTD   FPE FYLGAK +REVVF+VL
Sbjct: 242 YLDFGLAIPKLSVHGMISSLKELLELAPINKVMFSTDGCIFPETFYLGAKKSREVVFSVL 301

Query: 387 RDACIDGELSVPEAVEAAKDLFARNAIQFYKITI 420
           RD+C+DG+L+VPEAVEAAK + ARN+I FYKI +
Sbjct: 302 RDSCLDGDLTVPEAVEAAKGILARNSIHFYKINL 335


>Medtr1g062620.2 | glutamate-ammonia ligase-like protein | HC |
           chr1:27400951-27408864 | 20130731
          Length = 738

 Score =  417 bits (1073), Expect = e-117,   Method: Compositional matrix adjust.
 Identities = 200/285 (70%), Positives = 238/285 (83%), Gaps = 2/285 (0%)

Query: 136 FDDGIGMDKVQDIEWHKSFIPKVGRILRVEKLAEKILNEGLQGGSSWTLASFIGAFSSQL 195
            DDG  +DK+ DIEWHKSF+P VGRILR+EK+AE+IL++ L  GS WTL SF  AF S+L
Sbjct: 1   MDDGFELDKMHDIEWHKSFVPLVGRILRIEKVAEEILDQDLPDGSCWTLDSFTKAFVSKL 60

Query: 196 NSVVGEIYSLKSVVAYYAGLQINTNVTEEDAEEGLRQVLSAQMPIMITNKNLVDYLFLIS 255
            SV GEIY LKS+ AY +GL+INTNVT +DAEEGL QVL A+ P+ I NKNL+DY+FL S
Sbjct: 61  KSVAGEIYGLKSIAAYRSGLEINTNVTNQDAEEGLGQVLLARKPVRIANKNLIDYIFLQS 120

Query: 256 LEFAQSHDLPMQIHTGFGDGGYGLDLRLTNPLNLYNVLEDKRFSKSRIVLLHASYPFSKE 315
           L+ AQS+DLPMQIHTGFGD    LD+RL NPL+L +V EDKR+SKSRIVLLHASYPFS+E
Sbjct: 121 LDVAQSYDLPMQIHTGFGDKD--LDMRLANPLHLRSVFEDKRYSKSRIVLLHASYPFSRE 178

Query: 316 ASYLASVYSQVYLDFGLVIPKLSKHGMVTSVKGLLEQAPMSKVMFSTDSYAFPELFYLGA 375
           ASYLASVY QVYLDFGL IPKLS HGM++S+K LLE AP++KVMFSTD   FPE FYLGA
Sbjct: 179 ASYLASVYPQVYLDFGLAIPKLSVHGMISSLKELLELAPINKVMFSTDGCIFPETFYLGA 238

Query: 376 KNAREVVFTVLRDACIDGELSVPEAVEAAKDLFARNAIQFYKITI 420
           K +REVVF+VLRD+C+DG+L+VPEAVEAAK + ARN+I FYKI +
Sbjct: 239 KKSREVVFSVLRDSCLDGDLTVPEAVEAAKGILARNSIHFYKINL 283


>Medtr1g062620.4 | glutamate-ammonia ligase-like protein | HC |
           chr1:27403359-27408864 | 20130731
          Length = 684

 Score =  335 bits (858), Expect = 7e-92,   Method: Compositional matrix adjust.
 Identities = 161/224 (71%), Positives = 191/224 (85%), Gaps = 2/224 (0%)

Query: 197 SVVGEIYSLKSVVAYYAGLQINTNVTEEDAEEGLRQVLSAQMPIMITNKNLVDYLFLISL 256
           +V GEIY LKS+ AY +GL+INTNVT +DAEEGL QVL A+ P+ I NKNL+DY+FL SL
Sbjct: 8   TVAGEIYGLKSIAAYRSGLEINTNVTNQDAEEGLGQVLLARKPVRIANKNLIDYIFLQSL 67

Query: 257 EFAQSHDLPMQIHTGFGDGGYGLDLRLTNPLNLYNVLEDKRFSKSRIVLLHASYPFSKEA 316
           + AQS+DLPMQIHTGFGD    LD+RL NPL+L +V EDKR+SKSRIVLLHASYPFS+EA
Sbjct: 68  DVAQSYDLPMQIHTGFGDKD--LDMRLANPLHLRSVFEDKRYSKSRIVLLHASYPFSREA 125

Query: 317 SYLASVYSQVYLDFGLVIPKLSKHGMVTSVKGLLEQAPMSKVMFSTDSYAFPELFYLGAK 376
           SYLASVY QVYLDFGL IPKLS HGM++S+K LLE AP++KVMFSTD   FPE FYLGAK
Sbjct: 126 SYLASVYPQVYLDFGLAIPKLSVHGMISSLKELLELAPINKVMFSTDGCIFPETFYLGAK 185

Query: 377 NAREVVFTVLRDACIDGELSVPEAVEAAKDLFARNAIQFYKITI 420
            +REVVF+VLRD+C+DG+L+VPEAVEAAK + ARN+I FYKI +
Sbjct: 186 KSREVVFSVLRDSCLDGDLTVPEAVEAAKGILARNSIHFYKINL 229