Miyakogusa Predicted Gene

Lj5g3v0529110.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v0529110.1 Non Characterized Hit- tr|I1MWU9|I1MWU9_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.45199
PE,74.26,0,EF-hand,NULL; EF_hand_6,NULL; efhand,EF-hand; no
description,EF-hand-like domain; CALCIUM BINDING AT,CUFF.53211.1
         (482 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g012850.1 | calcium-binding EF hand protein | HC | chr1:26...   700   0.0  
Medtr3g106550.1 | calcium-binding EF hand protein | HC | chr3:49...   570   e-162
Medtr4g116190.1 | calcium-binding EF hand protein | HC | chr4:48...   529   e-150
Medtr3g106550.2 | calcium-binding EF hand protein | HC | chr3:49...   462   e-130

>Medtr1g012850.1 | calcium-binding EF hand protein | HC |
           chr1:2683696-2678294 | 20130731
          Length = 464

 Score =  700 bits (1806), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 361/482 (74%), Positives = 390/482 (80%), Gaps = 21/482 (4%)

Query: 1   MPSFSTLSRRSSHFTQHFPLQRFSIRHXXXXXXXXXXXXXXXXXFGRKLVSVIIAGTSLG 60
           M   STL   +SHF  HF +QR  I H                     L + IIAGTSLG
Sbjct: 1   MSFLSTLRNSTSHFFHHFSIQRLQILHTSSSPSLLNAR--------NGLFAAIIAGTSLG 52

Query: 61  LLLYS-EFDSASVKPSLSSFVDWSALTSDGQNXXXXXXXXXXXXXXLPESTGTLLFGDAY 119
            +LY+ +FDS   + S+SSFVD S   S                  LPE++G  LF    
Sbjct: 53  YVLYTTDFDSTHFQSSISSFVDRSTPNSPS----------FLTKFSLPETSGAFLFRG-- 100

Query: 120 RRKIFFNYEKRIRLRSPPEKVFEYFASCRTSDGELLMIPADLMRAVIPVFPPSESHHVRD 179
           +RK+FFNYEKRIRLRSPPEKVFEYFAS R+ +GE+LMIPADLMRAV+PVFPPSESH VRD
Sbjct: 101 KRKVFFNYEKRIRLRSPPEKVFEYFASHRSPEGEVLMIPADLMRAVVPVFPPSESHLVRD 160

Query: 180 GNLRGERSPGHLRCPRSEFFMLFDVNGDELISFKEYIFFVTLLSIPESSFSVAFKMFDVD 239
           G LRGERSPGHLRC  SEFFMLFDVN D LISFKEYIFFVTLLSIPESSFSVAFKMFDVD
Sbjct: 161 GYLRGERSPGHLRCSPSEFFMLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVD 220

Query: 240 NNGEISKEEFKKVMALMRSHHRQGVEHRDGLRTGLKVNDSVENGGLLEYFFGKDGKGCLQ 299
           NNGEI+KEEF+KVMALMRSHHRQGV+HRDGLRTGLKVNDSVENGGLLEYFFGKDGKGCLQ
Sbjct: 221 NNGEITKEEFQKVMALMRSHHRQGVQHRDGLRTGLKVNDSVENGGLLEYFFGKDGKGCLQ 280

Query: 300 HDKFVQFLRDLHDEILRLEFAHYDYKARKTISAKDFALSMVASADMSHIGKLLERVDELG 359
            DKFV FL+DLHDEILRLEF HYDYK+RK ISAKDFALSMVASADMSH+GKLLERVDEL 
Sbjct: 281 LDKFVIFLQDLHDEILRLEFDHYDYKSRKAISAKDFALSMVASADMSHLGKLLERVDELN 340

Query: 360 NDPLFKDVRITFEDFKSFAELRKKLFPLSLALFSFGKLNGLLTRDDFQRAASHVCGISLS 419
           N+P FKDVRI FEDFK+FAELRKKL PLSLALFSFGK+NGLLTRDDFQRAASHVCGISLS
Sbjct: 341 NEPCFKDVRIRFEDFKNFAELRKKLLPLSLALFSFGKVNGLLTRDDFQRAASHVCGISLS 400

Query: 420 DDVVEIVFHLFDTNWDGNLSSDEFVRVLHKRERDIVQPVETGIPGFLSCCWNCTDNISSL 479
           D+VVEIVFHLFDTN DGNLS+DEFVRVLHKRERDI  PVETG+ G LSCCWNC +NISS 
Sbjct: 401 DNVVEIVFHLFDTNRDGNLSTDEFVRVLHKRERDIGHPVETGMLGLLSCCWNCRENISSS 460

Query: 480 RF 481
           RF
Sbjct: 461 RF 462


>Medtr3g106550.1 | calcium-binding EF hand protein | HC |
           chr3:49246553-49249323 | 20130731
          Length = 455

 Score =  570 bits (1469), Expect = e-162,   Method: Compositional matrix adjust.
 Identities = 293/435 (67%), Positives = 339/435 (77%), Gaps = 35/435 (8%)

Query: 47  RKLVSVIIAGTSLGLLLY-----SEFDSASVKPSLSSFVDWSALTSDGQNXXXXXXXXXX 101
           RK VS ++ G++L  + Y     S FDSA +   L                         
Sbjct: 42  RKWVSALVVGSALAFVCYFSKTNSSFDSAFLNNILRK----------------------- 78

Query: 102 XXXXLPE-STGTLLFGDAYRRKIFFNYEKRIRLRSPPEKVFEYFASCRTSDGELLMIPAD 160
               LPE S   LLFGD+YR K+FFNYEKRIRL SPPEKVFEYFAS RT +GE+LM PAD
Sbjct: 79  --SSLPEGSNNNLLFGDSYRSKVFFNYEKRIRLHSPPEKVFEYFASYRTPEGEVLMKPAD 136

Query: 161 LMRAVIPVFPPSESHHVRDGNLRGERSPGHLRCPRSEFFMLFDVNGDELISFKEYIFFVT 220
           LMRAV+PVFPPSES+ VRDG+L GERSPGHL CP SEFF+LFDVNGD LISF+EY+F VT
Sbjct: 137 LMRAVVPVFPPSESNLVRDGSLEGERSPGHLFCPPSEFFLLFDVNGDGLISFREYLFLVT 196

Query: 221 LLSIPESSFSVAFKMFDVDNNGEISKEEFKKVMALMRSHHRQGVEHRDGLRTGLKVNDSV 280
           LLSIPES+FS  FKMFD+DN+GEI KEEFK+V+A MRSH + G+ HR       + + SV
Sbjct: 197 LLSIPESNFSAVFKMFDMDNDGEIDKEEFKRVVASMRSHSKHGI-HR---MYEQQTDASV 252

Query: 281 ENGGLLEYFFGKDGKGCLQHDKFVQFLRDLHDEILRLEFAHYDYKARKTISAKDFALSMV 340
           ENG ++EY FGKDGKG L+HDKFV+F+RDLHDEILRLEF HYD+K +KTI AKDFA S+V
Sbjct: 253 ENGRMVEYLFGKDGKGRLKHDKFVKFIRDLHDEILRLEFVHYDFKFQKTILAKDFAHSIV 312

Query: 341 ASADMSHIGKLLERVDELGNDPLFKDVRITFEDFKSFAELRKKLFPLSLALFSFGKLNGL 400
           ASAD++H+ KLLERVDEL NDP F  VRITFE+FK+FAELRKKL PLSLALFSF K+NGL
Sbjct: 313 ASADVNHLSKLLERVDELNNDPQFNSVRITFEEFKNFAELRKKLVPLSLALFSFAKVNGL 372

Query: 401 LTRDDFQRAASHVCGISLSDDVVEIVFHLFDTNWDGNLSSDEFVRVLHKRERDIVQPVET 460
           LTRDDFQRAAS VCG+SLSD+VVEIVFHLFDT+ DGNLSS+EFVRVL +RERD+ + VET
Sbjct: 373 LTRDDFQRAASSVCGLSLSDNVVEIVFHLFDTSGDGNLSSNEFVRVLQRRERDVGRSVET 432

Query: 461 GIPGFLSCCWNCTDN 475
           GI GFLSCCWNCTDN
Sbjct: 433 GIMGFLSCCWNCTDN 447


>Medtr4g116190.1 | calcium-binding EF hand protein | HC |
           chr4:48065719-48063074 | 20130731
          Length = 468

 Score =  529 bits (1362), Expect = e-150,   Method: Compositional matrix adjust.
 Identities = 267/430 (62%), Positives = 322/430 (74%), Gaps = 21/430 (4%)

Query: 45  FGRKLVS-VIIAGTSLGLLLYSEFDSASVKPSLSSFVDWSALTSDGQNXXXXXXXXXXXX 103
           F R + S  ++ GT+LG   +S   S S   SL SF D++      QN            
Sbjct: 55  FLRTITSGAVVVGTTLGFWYWSSLSSPSANNSLHSFSDYATEDQFQQNY----------- 103

Query: 104 XXLPESTGTLLFGDAYRRKIFFNYEKRIRLRSPPEKVFEYFASCRTSDGELLMIPADLMR 163
               ++    LF D YRR++FFNYEKR+RL+SPPEKVFEYFAS R   GE+ M PADLMR
Sbjct: 104 ----QNKSKFLFNDNYRRRVFFNYEKRLRLQSPPEKVFEYFASNRIPGGEVFMTPADLMR 159

Query: 164 AVIPVFPPSESHHVRDGNLRGERSPGHLRCPRSEFFMLFDVNGDELISFKEYIFFVTLLS 223
           A++PVFPPSES  VR+G+LRGE+ PG L C  SEFFMLFD + D LISF EYIFFVTLLS
Sbjct: 160 AIVPVFPPSESRRVREGSLRGEQFPGSLECDPSEFFMLFDTDNDGLISFAEYIFFVTLLS 219

Query: 224 IPESSFSVAFKMFDVDNNGEISKEEFKKVMALMRSHHRQGVEHRDGLRTGLKVNDSVENG 283
           IPESSFSVAFKMFD+DNNGEI KEEFKKVM+LMRS +RQG  HRDG R G+K    +ENG
Sbjct: 220 IPESSFSVAFKMFDIDNNGEIDKEEFKKVMSLMRSQNRQGANHRDGRRLGVKT--PIENG 277

Query: 284 GLLEYFFGKDGKGCLQHDKFVQFLRDLHDEILRLEFAHYDYKARKTISAKDFALSMVASA 343
           GLLEYFFGKDGK CLQHDKFVQFLR LHDEILRLEF+HYD+  R TISAKDFALS+VASA
Sbjct: 278 GLLEYFFGKDGKDCLQHDKFVQFLRQLHDEILRLEFSHYDHNKRGTISAKDFALSLVASA 337

Query: 344 DMSHIGKLLERVDELGNDPLFKDVRITFEDFKSFAELRKKLFPLSLALFSFGKLNGLLTR 403
           D++HI KLL+RVDEL NDP  +D RITFE+FK+FAELR+KL   SLA+FS+GK+NG+L +
Sbjct: 338 DINHINKLLDRVDELDNDPHIRDKRITFEEFKAFAELRRKLESFSLAIFSYGKVNGVLAK 397

Query: 404 DDFQRAASHVCGISLSDDVVEIVFHLFDTNWDGNLSSDEFVRVLHKRERDIVQPVETGIP 463
            DFQRAAS VCG+ ++++V++I+FH+FD N DG+LS+ EFVRV+ +RE DI      G  
Sbjct: 398 TDFQRAASQVCGVHVTENVLDIIFHVFDANRDGSLSASEFVRVIQRRE-DISSRF--GFG 454

Query: 464 GFLSCCWNCT 473
             ++C  NC 
Sbjct: 455 NLITCWLNCV 464


>Medtr3g106550.2 | calcium-binding EF hand protein | HC |
           chr3:49246553-49249323 | 20130731
          Length = 402

 Score =  462 bits (1188), Expect = e-130,   Method: Compositional matrix adjust.
 Identities = 244/377 (64%), Positives = 284/377 (75%), Gaps = 35/377 (9%)

Query: 47  RKLVSVIIAGTSLGLLLY-----SEFDSASVKPSLSSFVDWSALTSDGQNXXXXXXXXXX 101
           RK VS ++ G++L  + Y     S FDSA +   L                         
Sbjct: 42  RKWVSALVVGSALAFVCYFSKTNSSFDSAFLNNILRK----------------------- 78

Query: 102 XXXXLPE-STGTLLFGDAYRRKIFFNYEKRIRLRSPPEKVFEYFASCRTSDGELLMIPAD 160
               LPE S   LLFGD+YR K+FFNYEKRIRL SPPEKVFEYFAS RT +GE+LM PAD
Sbjct: 79  --SSLPEGSNNNLLFGDSYRSKVFFNYEKRIRLHSPPEKVFEYFASYRTPEGEVLMKPAD 136

Query: 161 LMRAVIPVFPPSESHHVRDGNLRGERSPGHLRCPRSEFFMLFDVNGDELISFKEYIFFVT 220
           LMRAV+PVFPPSES+ VRDG+L GERSPGHL CP SEFF+LFDVNGD LISF+EY+F VT
Sbjct: 137 LMRAVVPVFPPSESNLVRDGSLEGERSPGHLFCPPSEFFLLFDVNGDGLISFREYLFLVT 196

Query: 221 LLSIPESSFSVAFKMFDVDNNGEISKEEFKKVMALMRSHHRQGVEHRDGLRTGLKVNDSV 280
           LLSIPES+FS  FKMFD+DN+GEI KEEFK+V+A MRSH + G+ HR       + + SV
Sbjct: 197 LLSIPESNFSAVFKMFDMDNDGEIDKEEFKRVVASMRSHSKHGI-HR---MYEQQTDASV 252

Query: 281 ENGGLLEYFFGKDGKGCLQHDKFVQFLRDLHDEILRLEFAHYDYKARKTISAKDFALSMV 340
           ENG ++EY FGKDGKG L+HDKFV+F+RDLHDEILRLEF HYD+K +KTI AKDFA S+V
Sbjct: 253 ENGRMVEYLFGKDGKGRLKHDKFVKFIRDLHDEILRLEFVHYDFKFQKTILAKDFAHSIV 312

Query: 341 ASADMSHIGKLLERVDELGNDPLFKDVRITFEDFKSFAELRKKLFPLSLALFSFGKLNGL 400
           ASAD++H+ KLLERVDEL NDP F  VRITFE+FK+FAELRKKL PLSLALFSF K+NGL
Sbjct: 313 ASADVNHLSKLLERVDELNNDPQFNSVRITFEEFKNFAELRKKLVPLSLALFSFAKVNGL 372

Query: 401 LTRDDFQRAASHVCGIS 417
           LTRDDFQRAAS V  +S
Sbjct: 373 LTRDDFQRAASSVRHVS 389