Miyakogusa Predicted Gene

Lj4g3v3113090.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v3113090.1 CUFF.52327.1
         (694 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g105270.1 | vacuolar 14 Fab1-binding region protein | HC |...  1202   0.0  
Medtr1g032110.1 | VAC14-like protein | HC | chr1:11371433-113624...  1192   0.0  
Medtr1g032110.2 | VAC14-like protein | HC | chr1:11371422-113624...  1111   0.0  
Medtr8g105270.2 | vacuolar 14 Fab1-binding region protein | HC |...   867   0.0  
Medtr1g033440.1 | VAC14-like protein, putative | LC | chr1:12052...   340   3e-93

>Medtr8g105270.1 | vacuolar 14 Fab1-binding region protein | HC |
           chr8:44410257-44400965 | 20130731
          Length = 723

 Score = 1202 bits (3109), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 590/692 (85%), Positives = 620/692 (89%)

Query: 1   MADALSLIPAAVLRNLADKLYEKRKNAALEIEGIVKQLATGGDHDKIAAVVKLLTTEFTF 60
           MADALS IPAAVLRNLADKLYEKRKNAALEIE IVKQLA+ GDHDKIAAV+ L+T EFT+
Sbjct: 1   MADALSAIPAAVLRNLADKLYEKRKNAALEIENIVKQLASNGDHDKIAAVITLMTNEFTY 60

Query: 61  SPQANQRKGGLIGLAAATVGLASEAAQHLEHIVPPVLNSFSDQDSRVRYYACEALYNIAK 120
           SP AN RKGGLIGLAAATVGL SEA QHLE IVPPVL+SFSDQDSRVRYYACEALYNIAK
Sbjct: 61  SPHANHRKGGLIGLAAATVGLTSEAPQHLEQIVPPVLSSFSDQDSRVRYYACEALYNIAK 120

Query: 121 VVRGDFIVFFNQIFDALCKLSADSDANVQSAAHLLDRLVKDIVTESDQFSIEEFIPLLRE 180
           VVRGDFIVFFNQIFDALCKLSADSD NVQSAAHLLDRLVKDIVTESDQFSIEEFIPLLRE
Sbjct: 121 VVRGDFIVFFNQIFDALCKLSADSDPNVQSAAHLLDRLVKDIVTESDQFSIEEFIPLLRE 180

Query: 181 RMNVLNPYVRQFLVGWITVLDSVPDIDMXXXXXXXXXXXXNMLSDSSHEIRQQADSALSE 240
           RMNVLNP+VRQFLVGWITVLDSVPDIDM            NMLSDSSHEIRQQADSALSE
Sbjct: 181 RMNVLNPFVRQFLVGWITVLDSVPDIDMLGFLPDFLDGLFNMLSDSSHEIRQQADSALSE 240

Query: 241 FLQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLTAITWINEFVKLGGDQLVPYYADILGA 300
           FLQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLT+ITWINEFVKLGGDQLVPYYADILGA
Sbjct: 241 FLQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLTSITWINEFVKLGGDQLVPYYADILGA 300

Query: 301 ILPCISDKEEKIRVVARETNEELRTIKADPAEGFDVGAILSIARRQLSSDWEATRIEALH 360
           ILPCISDKEEKIRVVARETNE LR IKADPAE  DV +ILSIARRQLSS+WEATRIEALH
Sbjct: 301 ILPCISDKEEKIRVVARETNEALRGIKADPAEACDVESILSIARRQLSSEWEATRIEALH 360

Query: 361 WISTLLNKHRTEVLQYLNDIFDSLLKALSDPSDEVVLLVLDVHACIAKDPQHFRQLVVFL 420
           WI+TLLNK+RTE LQ+LNDIFD+LLKALSDPSDEVVLLVLDVHACIAKDPQHFRQLVVFL
Sbjct: 361 WIATLLNKYRTECLQFLNDIFDTLLKALSDPSDEVVLLVLDVHACIAKDPQHFRQLVVFL 420

Query: 421 VHNFRIDNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDLDFASIMVQAXXXXXX 480
           +HNFRIDNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDL+FASIMVQA      
Sbjct: 421 MHNFRIDNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDLNFASIMVQALNLILL 480

Query: 481 XXXXXXXXRDLLKQSLVNPAGKDLYVSLYASWCHSPMAIISLCLLAQTYQHASTVIQSLV 540
                   R LLKQSLVNPAGKDLYVSLYASWCHSPMAIISLC LAQTYQHASTVI+SL 
Sbjct: 481 TSSELSEMRVLLKQSLVNPAGKDLYVSLYASWCHSPMAIISLCFLAQTYQHASTVIESLA 540

Query: 541 EEDINVKLLVQLDKLIRLLETPVFTYLRLQLLEPGRYPWLFKALYGLLMLLPQQSAAFKI 600
           EEDINVK LVQLDKLIRLLETPVF YLRLQLLEPGRY WLFKALYGLLMLLPQQSAAFK+
Sbjct: 541 EEDINVKFLVQLDKLIRLLETPVFAYLRLQLLEPGRYTWLFKALYGLLMLLPQQSAAFKV 600

Query: 601 LKTRLKAVPSFSFNGEQLKKTSSGNPYQFLHHMSGGSHTSEDGDITADSGNSHNGINFAA 660
           LKTRLKAVPS+SFNG+ LKK+S+GNPYQFLHH+SGGSH +EDG++  D+G+ HNGINF A
Sbjct: 601 LKTRLKAVPSYSFNGDHLKKSSNGNPYQFLHHISGGSHITEDGNVAVDNGDPHNGINFVA 660

Query: 661 RLXXXXXXXXXHRVHMKVQTLSRKNSPSLSKE 692
           RL         HR   + Q  +RK S S+SKE
Sbjct: 661 RLHQFHQMQQQHREFFRNQAQTRKTSTSVSKE 692


>Medtr1g032110.1 | VAC14-like protein | HC | chr1:11371433-11362455
           | 20130731
          Length = 733

 Score = 1192 bits (3084), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 589/693 (84%), Positives = 614/693 (88%), Gaps = 2/693 (0%)

Query: 1   MADALSLIPAAVLRNLADKLYEKRKNAALEIEGIVKQLATGGDHDKIAAVVKLLTTEFTF 60
           MADAL+++PAAVLRNLADKLYEKRKNAALE+EGIVKQLA+ GDHDKI AV+ LLTTEFT+
Sbjct: 1   MADALTVMPAAVLRNLADKLYEKRKNAALEVEGIVKQLASAGDHDKITAVITLLTTEFTY 60

Query: 61  SPQANQRKGGLIGLAAATVGLASEAAQHLEHIVPPVLNSFSDQDSRVRYYACEALYNIAK 120
           SPQAN RKGGLI LAAATVGL +EAAQHLE IVPPVLNSFSDQDSRVRYYACEALYNIAK
Sbjct: 61  SPQANHRKGGLISLAAATVGLTNEAAQHLEQIVPPVLNSFSDQDSRVRYYACEALYNIAK 120

Query: 121 VVRGDFIVFFNQIFDALCKLSADSDANVQSAAHLLDRLVKDIVTESDQFSIEEFIPLLRE 180
           VVRGDFIVFFNQIFDALCKLSADSDANVQSAAHLLDRLVKDIVTESDQFSIEEFIPLLRE
Sbjct: 121 VVRGDFIVFFNQIFDALCKLSADSDANVQSAAHLLDRLVKDIVTESDQFSIEEFIPLLRE 180

Query: 181 RMNVLNPYVRQFLVGWITVLDSVPDIDMXXXXXXXXXXXXNMLSDSSHEIRQQADSALSE 240
           RMNVLNPYVRQFLVGWITVLDSVPDIDM            NMLSDSSHEIRQQADSALSE
Sbjct: 181 RMNVLNPYVRQFLVGWITVLDSVPDIDMLGFLPDFLDGLFNMLSDSSHEIRQQADSALSE 240

Query: 241 FLQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLTAITWINEFVKLGGDQLVPYYADILGA 300
           FLQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLTAITWINEFVKLGGDQLVPYYADILGA
Sbjct: 241 FLQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLTAITWINEFVKLGGDQLVPYYADILGA 300

Query: 301 ILPCISDKEEKIRVVARETNEELRTIKADPAEGFDVGAILSIARRQLSSDWEATRIEALH 360
           ILPCISDKEEKIRVVARETNEELR I ADPAE FDVGAILSIARRQLSS+WEATRIEALH
Sbjct: 301 ILPCISDKEEKIRVVARETNEELRAINADPAEAFDVGAILSIARRQLSSEWEATRIEALH 360

Query: 361 WISTLLNKHRTEVLQYLNDIFDSLLKALSDPSDEVVLLVLDVHACIAKDPQHFRQLVVFL 420
           WISTLLN+HR EVL YLNDIFD+LLKALSDPSDEVVLLVLDVHACIA+DPQHFRQLVVFL
Sbjct: 361 WISTLLNRHRIEVLTYLNDIFDTLLKALSDPSDEVVLLVLDVHACIARDPQHFRQLVVFL 420

Query: 421 VHNFRIDNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDLDFASIMVQAXXXXXX 480
           VHNFR+DNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDLDFASIMVQA      
Sbjct: 421 VHNFRLDNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDLDFASIMVQALNLILL 480

Query: 481 XXXXXXXXRDLLKQSLVNPAGKDLYVSLYASWCHSPMAIISLCLLAQTYQHASTVIQSLV 540
                   RDLLKQSLVNPAGKDLY+SLYASWCHSPMAIISLC +AQTY+HAS VIQSL 
Sbjct: 481 TSSELSEIRDLLKQSLVNPAGKDLYISLYASWCHSPMAIISLCFVAQTYRHASAVIQSLE 540

Query: 541 EEDINVKLLVQLDKLIRLLETPVFTYLRLQLLEPGRYPWLFKALYGLLMLLPQQSAAFKI 600
           EEDINVK LVQLDKLIRLLETP+F YLRLQLL+P RY WLFKALYGLLMLLPQQSAAFKI
Sbjct: 541 EEDINVKFLVQLDKLIRLLETPIFAYLRLQLLDPARYLWLFKALYGLLMLLPQQSAAFKI 600

Query: 601 LKTRLKAVPSFSFNGEQLKKTSSGNPYQFLHHMSGGSHTSEDGDITADSGNSHNGINFAA 660
           LKTRLKAVPS+SFN  QL +  SG+ YQFL  M  G  T EDGD+T D G+S+N INFAA
Sbjct: 601 LKTRLKAVPSYSFNRAQLNREPSGDYYQFLPQMPDG--TKEDGDVTEDGGSSYNAINFAA 658

Query: 661 RLXXXXXXXXXHRVHMKVQTLSRKNSPSLSKEA 693
           RL         HRVH K +  SR  S SL K A
Sbjct: 659 RLQQFQKMQQQHRVHTKSRRTSRSLSTSLPKVA 691


>Medtr1g032110.2 | VAC14-like protein | HC | chr1:11371422-11362455
           | 20130731
          Length = 702

 Score = 1111 bits (2873), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 558/693 (80%), Positives = 583/693 (84%), Gaps = 33/693 (4%)

Query: 1   MADALSLIPAAVLRNLADKLYEKRKNAALEIEGIVKQLATGGDHDKIAAVVKLLTTEFTF 60
           MADAL+++PAAVLRNLADKLYEKRKNAALE+EGIVKQLA+ GDHDKI AV+ LLTTEFT+
Sbjct: 1   MADALTVMPAAVLRNLADKLYEKRKNAALEVEGIVKQLASAGDHDKITAVITLLTTEFTY 60

Query: 61  SPQANQRKGGLIGLAAATVGLASEAAQHLEHIVPPVLNSFSDQDSRVRYYACEALYNIAK 120
           SPQAN RKGGLI LAAATVGL +EAAQHLE IVPPVLNSFSDQDSRVRYYACEALYNIAK
Sbjct: 61  SPQANHRKGGLISLAAATVGLTNEAAQHLEQIVPPVLNSFSDQDSRVRYYACEALYNIAK 120

Query: 121 VVRGDFIVFFNQIFDALCKLSADSDANVQSAAHLLDRLVKDIVTESDQFSIEEFIPLLRE 180
           VVRGDFIVFFNQIFDALCKLSADSDANVQSAAHLLDRLVK                    
Sbjct: 121 VVRGDFIVFFNQIFDALCKLSADSDANVQSAAHLLDRLVK-------------------- 160

Query: 181 RMNVLNPYVRQFLVGWITVLDSVPDIDMXXXXXXXXXXXXNMLSDSSHEIRQQADSALSE 240
                      FLVGWITVLDSVPDIDM            NMLSDSSHEIRQQADSALSE
Sbjct: 161 -----------FLVGWITVLDSVPDIDMLGFLPDFLDGLFNMLSDSSHEIRQQADSALSE 209

Query: 241 FLQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLTAITWINEFVKLGGDQLVPYYADILGA 300
           FLQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLTAITWINEFVKLGGDQLVPYYADILGA
Sbjct: 210 FLQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLTAITWINEFVKLGGDQLVPYYADILGA 269

Query: 301 ILPCISDKEEKIRVVARETNEELRTIKADPAEGFDVGAILSIARRQLSSDWEATRIEALH 360
           ILPCISDKEEKIRVVARETNEELR I ADPAE FDVGAILSIARRQLSS+WEATRIEALH
Sbjct: 270 ILPCISDKEEKIRVVARETNEELRAINADPAEAFDVGAILSIARRQLSSEWEATRIEALH 329

Query: 361 WISTLLNKHRTEVLQYLNDIFDSLLKALSDPSDEVVLLVLDVHACIAKDPQHFRQLVVFL 420
           WISTLLN+HR EVL YLNDIFD+LLKALSDPSDEVVLLVLDVHACIA+DPQHFRQLVVFL
Sbjct: 330 WISTLLNRHRIEVLTYLNDIFDTLLKALSDPSDEVVLLVLDVHACIARDPQHFRQLVVFL 389

Query: 421 VHNFRIDNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDLDFASIMVQAXXXXXX 480
           VHNFR+DNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDLDFASIMVQA      
Sbjct: 390 VHNFRLDNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDLDFASIMVQALNLILL 449

Query: 481 XXXXXXXXRDLLKQSLVNPAGKDLYVSLYASWCHSPMAIISLCLLAQTYQHASTVIQSLV 540
                   RDLLKQSLVNPAGKDLY+SLYASWCHSPMAIISLC +AQTY+HAS VIQSL 
Sbjct: 450 TSSELSEIRDLLKQSLVNPAGKDLYISLYASWCHSPMAIISLCFVAQTYRHASAVIQSLE 509

Query: 541 EEDINVKLLVQLDKLIRLLETPVFTYLRLQLLEPGRYPWLFKALYGLLMLLPQQSAAFKI 600
           EEDINVK LVQLDKLIRLLETP+F YLRLQLL+P RY WLFKALYGLLMLLPQQSAAFKI
Sbjct: 510 EEDINVKFLVQLDKLIRLLETPIFAYLRLQLLDPARYLWLFKALYGLLMLLPQQSAAFKI 569

Query: 601 LKTRLKAVPSFSFNGEQLKKTSSGNPYQFLHHMSGGSHTSEDGDITADSGNSHNGINFAA 660
           LKTRLKAVPS+SFN  QL +  SG+ YQFL  M  G  T EDGD+T D G+S+N INFAA
Sbjct: 570 LKTRLKAVPSYSFNRAQLNREPSGDYYQFLPQMPDG--TKEDGDVTEDGGSSYNAINFAA 627

Query: 661 RLXXXXXXXXXHRVHMKVQTLSRKNSPSLSKEA 693
           RL         HRVH K +  SR  S SL K A
Sbjct: 628 RLQQFQKMQQQHRVHTKSRRTSRSLSTSLPKVA 660


>Medtr8g105270.2 | vacuolar 14 Fab1-binding region protein | HC |
           chr8:44407857-44400965 | 20130731
          Length = 542

 Score =  867 bits (2240), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 425/511 (83%), Positives = 450/511 (88%)

Query: 182 MNVLNPYVRQFLVGWITVLDSVPDIDMXXXXXXXXXXXXNMLSDSSHEIRQQADSALSEF 241
           MNVLNP+VRQFLVGWITVLDSVPDIDM            NMLSDSSHEIRQQADSALSEF
Sbjct: 1   MNVLNPFVRQFLVGWITVLDSVPDIDMLGFLPDFLDGLFNMLSDSSHEIRQQADSALSEF 60

Query: 242 LQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLTAITWINEFVKLGGDQLVPYYADILGAI 301
           LQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLT+ITWINEFVKLGGDQLVPYYADILGAI
Sbjct: 61  LQEIKNSPSVDYGRMAEILVQRAGSPDEFTRLTSITWINEFVKLGGDQLVPYYADILGAI 120

Query: 302 LPCISDKEEKIRVVARETNEELRTIKADPAEGFDVGAILSIARRQLSSDWEATRIEALHW 361
           LPCISDKEEKIRVVARETNE LR IKADPAE  DV +ILSIARRQLSS+WEATRIEALHW
Sbjct: 121 LPCISDKEEKIRVVARETNEALRGIKADPAEACDVESILSIARRQLSSEWEATRIEALHW 180

Query: 362 ISTLLNKHRTEVLQYLNDIFDSLLKALSDPSDEVVLLVLDVHACIAKDPQHFRQLVVFLV 421
           I+TLLNK+RTE LQ+LNDIFD+LLKALSDPSDEVVLLVLDVHACIAKDPQHFRQLVVFL+
Sbjct: 181 IATLLNKYRTECLQFLNDIFDTLLKALSDPSDEVVLLVLDVHACIAKDPQHFRQLVVFLM 240

Query: 422 HNFRIDNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDLDFASIMVQAXXXXXXX 481
           HNFRIDNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDL+FASIMVQA       
Sbjct: 241 HNFRIDNSLLEKRGALIIRRLCVLLNAERVYRELSTILEGESDLNFASIMVQALNLILLT 300

Query: 482 XXXXXXXRDLLKQSLVNPAGKDLYVSLYASWCHSPMAIISLCLLAQTYQHASTVIQSLVE 541
                  R LLKQSLVNPAGKDLYVSLYASWCHSPMAIISLC LAQTYQHASTVI+SL E
Sbjct: 301 SSELSEMRVLLKQSLVNPAGKDLYVSLYASWCHSPMAIISLCFLAQTYQHASTVIESLAE 360

Query: 542 EDINVKLLVQLDKLIRLLETPVFTYLRLQLLEPGRYPWLFKALYGLLMLLPQQSAAFKIL 601
           EDINVK LVQLDKLIRLLETPVF YLRLQLLEPGRY WLFKALYGLLMLLPQQSAAFK+L
Sbjct: 361 EDINVKFLVQLDKLIRLLETPVFAYLRLQLLEPGRYTWLFKALYGLLMLLPQQSAAFKVL 420

Query: 602 KTRLKAVPSFSFNGEQLKKTSSGNPYQFLHHMSGGSHTSEDGDITADSGNSHNGINFAAR 661
           KTRLKAVPS+SFNG+ LKK+S+GNPYQFLHH+SGGSH +EDG++  D+G+ HNGINF AR
Sbjct: 421 KTRLKAVPSYSFNGDHLKKSSNGNPYQFLHHISGGSHITEDGNVAVDNGDPHNGINFVAR 480

Query: 662 LXXXXXXXXXHRVHMKVQTLSRKNSPSLSKE 692
           L         HR   + Q  +RK S S+SKE
Sbjct: 481 LHQFHQMQQQHREFFRNQAQTRKTSTSVSKE 511


>Medtr1g033440.1 | VAC14-like protein, putative | LC |
           chr1:12052407-12048536 | 20130731
          Length = 376

 Score =  340 bits (871), Expect = 3e-93,   Method: Compositional matrix adjust.
 Identities = 164/189 (86%), Positives = 174/189 (92%)

Query: 250 SVDYGRMAEILVQRAGSPDEFTRLTAITWINEFVKLGGDQLVPYYADILGAILPCISDKE 309
           SVDYGRMAEILVQRAGSPDEFT LTAITWINEFVKLGGDQLVPYYADILGAILPCISDKE
Sbjct: 56  SVDYGRMAEILVQRAGSPDEFTSLTAITWINEFVKLGGDQLVPYYADILGAILPCISDKE 115

Query: 310 EKIRVVARETNEELRTIKADPAEGFDVGAILSIARRQLSSDWEATRIEALHWISTLLNKH 369
           EKIRVVARET EELR I ADPAE FDVGAILSIARR  SS+WEATRIEALHWISTLLN+H
Sbjct: 116 EKIRVVARETIEELRAINADPAEAFDVGAILSIARRCNSSEWEATRIEALHWISTLLNRH 175

Query: 370 RTEVLQYLNDIFDSLLKALSDPSDEVVLLVLDVHACIAKDPQHFRQLVVFLVHNFRIDNS 429
             EVL YLNDIFD+LLKALSDPSDEVVLLVLDVH CI++DP+H RQLVVFLVHNFR+DNS
Sbjct: 176 CIEVLTYLNDIFDTLLKALSDPSDEVVLLVLDVHTCISRDPRHLRQLVVFLVHNFRLDNS 235

Query: 430 LLEKRGALI 438
           LLE   +++
Sbjct: 236 LLENFQSIV 244