Miyakogusa Predicted Gene

Lj4g3v3019490.1
Show Alignment:  
BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v3019490.1 Non Characterized Hit- tr|I1KPT8|I1KPT8_SOYBN
Uncharacterized protein OS=Glycine max PE=3 SV=1,86.53,0,ZINC
PROTEASE-RELATED,NULL; METALLOPROTEASE,NULL; no description,Peptidase
M16, core; LuxS/MPP-like ,CUFF.52150.1
         (930 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g101950.1 | peptidase M16 inactive domain protein | HC | c...  1517   0.0  
Medtr5g007380.2 | insulinase (peptidase family M16) | HC | chr5:...    99   2e-20
Medtr5g007380.1 | insulinase (peptidase family M16) | HC | chr5:...    99   2e-20
Medtr7g053330.1 | processing peptidase | HC | chr7:18766364-1876...    50   9e-06

>Medtr8g101950.1 | peptidase M16 inactive domain protein | HC |
           chr8:42848743-42859442 | 20130731
          Length = 978

 Score = 1517 bits (3927), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 754/893 (84%), Positives = 787/893 (88%), Gaps = 42/893 (4%)

Query: 38  GATPIPKKQRFRSLKLVNVDMEQLLPHQPVGVDYGTLDNGLRYYVRCNSKPRMXXXXXXX 97
            A PI +KQ FRSLKLV+ DM QLL  QPVGVD+GTLDNGLRYYVRCNSKPRM       
Sbjct: 8   AAAPISRKQGFRSLKLVSTDMNQLLSDQPVGVDFGTLDNGLRYYVRCNSKPRMRAALALA 67

Query: 98  XXXGSVLEEEDERGVAHIVEHLAFSATTKYTNHDIVKFLESIGAEFGACQNAVTSSDDTV 157
              GSVLEEEDERGVAHIVEHLAFSAT +Y NHDIVKFLESIGAEFGACQNAVTSSDDTV
Sbjct: 68  VRVGSVLEEEDERGVAHIVEHLAFSATKRYNNHDIVKFLESIGAEFGACQNAVTSSDDTV 127

Query: 158 YELLVPVDKPELLSQAISILAEFSSEIRVSKDDLEKERGAVMEEYRGSRNATGRLQDAHW 217
           YELLVPVDKPELLSQAISILAEFSSEIRVSKDDLEKERGAVMEEYRGSRNATGRLQDAHW
Sbjct: 128 YELLVPVDKPELLSQAISILAEFSSEIRVSKDDLEKERGAVMEEYRGSRNATGRLQDAHW 187

Query: 218 ILLMEGSKYAERLPIGLEKVIRTVPHETVKNFYKKWYHLCNMAVIAVGDFPDTQSVVELI 277
            LLMEGSKYAERLPIGLEKVIRTV  ETV++FYKKWYHLCNMAVIAVG      SVVELI
Sbjct: 188 TLLMEGSKYAERLPIGLEKVIRTVSPETVRHFYKKWYHLCNMAVIAVG------SVVELI 241

Query: 278 KTHFGQKIPAPDPPLIPTFHIPSHDEPRFSCFVESEAGGSAVMISYKMQAEELKTVKDYR 337
           K HFGQKIPAPDPP IPTF +PSHD+PRFSCFVESEA GSAVMISYKM A ELK      
Sbjct: 242 KVHFGQKIPAPDPPPIPTFQVPSHDDPRFSCFVESEAAGSAVMISYKMPANELK------ 295

Query: 338 NLLAESMFFHALNQRFFKISRRKDPPFFSCSASADNLVRPLKANIMTSSCKGKGTLQALE 397
                                         + SAD LVRP+K NI+TSSC+GKGTL+ALE
Sbjct: 296 ------------------------------NTSADVLVRPVKTNIITSSCRGKGTLEALE 325

Query: 398 SMLIEVARVRLHGFSEREVSIVRSLLMSEIESAFLERDQVQSTSMRDEYLQHFLHNEPVV 457
           SML EVARVRLHGFSEREVSIVR+LLMSEIESA+LERDQVQSTS+RDEYLQHFLHNEPVV
Sbjct: 326 SMLTEVARVRLHGFSEREVSIVRALLMSEIESAYLERDQVQSTSLRDEYLQHFLHNEPVV 385

Query: 458 GIEYEAQLQKTLLPHISALEVSKCSERLRTSNSCVIKTIEPQAFAXXXXXXXXXXXXXXX 517
           GIEYEAQLQKTLLPHISALEVSK SE+LRTS SCVIKT+EP+AFA               
Sbjct: 386 GIEYEAQLQKTLLPHISALEVSKYSEKLRTSCSCVIKTMEPRAFAVFDDLKNVVKKVNLL 445

Query: 518 XXXGEISSWDDEYIPEEIVTTKPNMGHVMKELEYSNIGATELVLSNGMRVCYKRTDFLDD 577
              G IS WDD++IPEEIVTTKPNMGHV+KELEYSNIGATEL+LSNGMR+CYKRTDFLDD
Sbjct: 446 EEEGGISLWDDDHIPEEIVTTKPNMGHVVKELEYSNIGATELILSNGMRICYKRTDFLDD 505

Query: 578 QVIFTGYSYGGLSELPENEYFSCSMGPTIAGEIGVFGYRPSVLMDMLAGKRAEVGTKIGA 637
           QVIFTGYSYGGLSELPE+EYFSCSMGPTIAGEIGVFGYRPSVLMDMLAGKRAEVGTKIGA
Sbjct: 506 QVIFTGYSYGGLSELPESEYFSCSMGPTIAGEIGVFGYRPSVLMDMLAGKRAEVGTKIGA 565

Query: 638 YMRTFYGDCSPSDLETALQLVYQLFTTNLTPGEESVKIVMQMAEEAVCAQDRDPYTAFTN 697
           YMRTFYGDCSPSDLET LQLVYQLFTTNLTP EE+VKIVMQMAEEAVCAQDRDPYTAFTN
Sbjct: 566 YMRTFYGDCSPSDLETGLQLVYQLFTTNLTPDEENVKIVMQMAEEAVCAQDRDPYTAFTN 625

Query: 698 RVKELNYGNSYFFRPVKKADLQKVDPVKACEYFSKCFRDPSTFTVVIVGNIDSTIALPLI 757
           RVKELNYGNSYFFRP+KK DLQKVDP++ACEYFSKCFRDPS FTVVIVGNID TIALPLI
Sbjct: 626 RVKELNYGNSYFFRPIKKCDLQKVDPLEACEYFSKCFRDPSAFTVVIVGNIDPTIALPLI 685

Query: 758 LQYLGGILKPPEPIMHFNRDELKGLPFTFPTTIHREVVRSPMVEAQCLVQICFPVELKNG 817
           LQYLGGI KPPEPIMHFNRD+LKGLPFTFPT IHREVVRSPMVEAQCLVQICFPVELKNG
Sbjct: 686 LQYLGGIPKPPEPIMHFNRDDLKGLPFTFPTAIHREVVRSPMVEAQCLVQICFPVELKNG 745

Query: 818 TMVEEIHFVGFLSKLLETKIMQVLRFKHGQIYSVGVSVFLGGNKPSRTGDARGDISINFS 877
           TMVEEIHFVGFLSKLLETKIMQVLRFKHGQIYSVGVSVFLGGNKPSRTG  RGDISINFS
Sbjct: 746 TMVEEIHFVGFLSKLLETKIMQVLRFKHGQIYSVGVSVFLGGNKPSRTGIVRGDISINFS 805

Query: 878 CDPEISSKLVDLALDEMLRLQEEGPSEQDVSTVLEIEQRAHENGLQENYYWLD 930
           CDPEISSKLVD+ALDEMLRLQ+EGP+EQDVSTVLEIEQRAHENGLQENYYWLD
Sbjct: 806 CDPEISSKLVDIALDEMLRLQDEGPTEQDVSTVLEIEQRAHENGLQENYYWLD 858


>Medtr5g007380.2 | insulinase (peptidase family M16) | HC |
           chr5:1336812-1323954 | 20130731
          Length = 1258

 Score = 99.0 bits (245), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 69/225 (30%), Positives = 104/225 (46%), Gaps = 14/225 (6%)

Query: 62  LPHQPVGVDYGTLDNGLRYYVRCNSKPRMXXXXXXXXXXGSVLEEEDERGVAHIVEHLAF 121
           LP  P  +  G L NGLRY +  N  P            GS+ E +DE+G+AH++EH+AF
Sbjct: 186 LPSHP-KLHRGQLKNGLRYLILPNKVPPTRFEAHMEVHAGSIDEGDDEQGIAHMIEHVAF 244

Query: 122 SATTKYTNHDIVKFLESIGAEFGACQNAVTSSDDTVYELLVPV---DKPELLSQAISILA 178
             + K       K L +     GA  NA T    TV+ +  P    D  +LL   +  L 
Sbjct: 245 LGSKKRE-----KLLGT-----GARSNAYTDFHHTVFHIHAPTTTKDSDDLLPSVLDALN 294

Query: 179 EFSSEIRVSKDDLEKERGAVMEEYRGSRNATGRLQDAHWILLMEGSKYAERLPIGLEKVI 238
           E +   +     +EKER A++ E +       R+       L   +K ++R PIGLE+ I
Sbjct: 295 EIAFHPKFLSSRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQI 354

Query: 239 RTVPHETVKNFYKKWYHLCNMAVIAVGDFPDTQSVVELIKTHFGQ 283
           +    E ++ F+++WY   N  +  VGD  +    V  I+  FGQ
Sbjct: 355 KKWDAEKIRKFHERWYFPANATLYIVGDIDNIPKTVGQIEAVFGQ 399


>Medtr5g007380.1 | insulinase (peptidase family M16) | HC |
           chr5:1336812-1323954 | 20130731
          Length = 1258

 Score = 99.0 bits (245), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 69/225 (30%), Positives = 104/225 (46%), Gaps = 14/225 (6%)

Query: 62  LPHQPVGVDYGTLDNGLRYYVRCNSKPRMXXXXXXXXXXGSVLEEEDERGVAHIVEHLAF 121
           LP  P  +  G L NGLRY +  N  P            GS+ E +DE+G+AH++EH+AF
Sbjct: 186 LPSHP-KLHRGQLKNGLRYLILPNKVPPTRFEAHMEVHAGSIDEGDDEQGIAHMIEHVAF 244

Query: 122 SATTKYTNHDIVKFLESIGAEFGACQNAVTSSDDTVYELLVPV---DKPELLSQAISILA 178
             + K       K L +     GA  NA T    TV+ +  P    D  +LL   +  L 
Sbjct: 245 LGSKKRE-----KLLGT-----GARSNAYTDFHHTVFHIHAPTTTKDSDDLLPSVLDALN 294

Query: 179 EFSSEIRVSKDDLEKERGAVMEEYRGSRNATGRLQDAHWILLMEGSKYAERLPIGLEKVI 238
           E +   +     +EKER A++ E +       R+       L   +K ++R PIGLE+ I
Sbjct: 295 EIAFHPKFLSSRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQI 354

Query: 239 RTVPHETVKNFYKKWYHLCNMAVIAVGDFPDTQSVVELIKTHFGQ 283
           +    E ++ F+++WY   N  +  VGD  +    V  I+  FGQ
Sbjct: 355 KKWDAEKIRKFHERWYFPANATLYIVGDIDNIPKTVGQIEAVFGQ 399


>Medtr7g053330.1 | processing peptidase | HC |
           chr7:18766364-18760054 | 20130731
          Length = 532

 Score = 50.1 bits (118), Expect = 9e-06,   Method: Compositional matrix adjust.
 Identities = 54/212 (25%), Positives = 88/212 (41%), Gaps = 15/212 (7%)

Query: 73  TLDNGLRYYVRCNSKPRMXXXXXXXXXXGSVLEEEDERGVAHIVEHLAFSATTKYTNHDI 132
           TL NGLR      S   M          GS  E E+  G AH +EH+ F  T +    D+
Sbjct: 102 TLPNGLRVATES-SLSAMTATVGVWIDAGSRFETEESNGTAHFLEHMIFKGTERRNARDL 160

Query: 133 VKFLESIGAEFGACQNAVTSSDDTVYELLVPV-DKPELLSQAISILAEFSSEIRVSKDDL 191
            + +E++G       NA TS + T Y   V   D P     A+ ILA+     + +++ +
Sbjct: 161 EEEIENMGGHL----NAYTSREQTTYYAKVSQGDVP----VALDILADILQNSKFNENRI 212

Query: 192 EKERGAVMEEYRGSRNATGRLQDAHWILLMEGSKYAE--RLPIGLEKVIRTVPHETVKNF 249
            +ER  ++ E       T  +   H  L     +Y    R  +G  + I+T+    ++++
Sbjct: 213 SRERDVILREMEEVEGQTEEVIFDH--LHATAFQYTPLGRTILGPAQNIKTITKAHLQDY 270

Query: 250 YKKWYHLCNMAVIAVGDFPDTQSVVELIKTHF 281
            +  Y    M + A G     +  VE +K  F
Sbjct: 271 IQTHYTAPRMVIAASGAVKH-EDFVEQVKKLF 301