Miyakogusa Predicted Gene

Lj4g3v3014900.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v3014900.1 Non Characterized Hit- tr|E1ZK35|E1ZK35_CHLVA
Putative uncharacterized protein OS=Chlorella
variabil,34.05,1e-17,PseudoU_synth_1,Pseudouridine synthase I, TruA,
alpha/beta domain; no description,Pseudouridine synt,CUFF.52095.1
         (390 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g101250.1 | tRNA pseudouridine synthase | HC | chr8:424998...   539   e-153
Medtr8g101250.2 | tRNA pseudouridine synthase | HC | chr8:424998...   443   e-124
Medtr4g131220.1 | tRNA pseudouridine synthase A, related protein...    63   6e-10
Medtr4g131270.1 | tRNA pseudouridine synthase A, related protein...    62   6e-10
Medtr4g123970.1 | tRNA pseudouridine synthase A | HC | chr4:5111...    55   1e-07

>Medtr8g101250.1 | tRNA pseudouridine synthase | HC |
           chr8:42499859-42506686 | 20130731
          Length = 435

 Score =  539 bits (1388), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 258/344 (75%), Positives = 293/344 (85%), Gaps = 5/344 (1%)

Query: 42  NYAHYNHTDPCKHARWNARESYEFMYARPWQRVNDFYLNAVLGNLSLPLLFGAEKIHDHN 101
           ++ HYNHT P K+ARWNARES+EFMYARPWQRVN FY N V GN SLPLLF ++ +  H+
Sbjct: 45  HHVHYNHTHPSKYARWNARESFEFMYARPWQRVNHFYSNTVRGNFSLPLLFQSQTLPVHD 104

Query: 102 NEPGIPAVSDKPEFESVASRGRGGRWARLTFKIVLSYHGGSFDGWQKQPGLNTVQSVIEG 161
           +   +P +S   EF+S AS+ R G+WAR  FKI+LSYHGGSFDGWQKQP LNTVQSV+E 
Sbjct: 105 SR--VPEIS---EFQSGASKDRSGKWARFNFKILLSYHGGSFDGWQKQPNLNTVQSVVEV 159

Query: 162 PLGTFVDENKTQQLKDRGLPIEGSVTVAGRTDKGVTALQQVCSFYTWKKDVKPRDIEDAI 221
            LG FVDENKTQ LKD+GLPI+G   VAGRTDKGVTALQQVCSFYTWKKD+KPR+IEDAI
Sbjct: 160 SLGKFVDENKTQLLKDKGLPIQGCAAVAGRTDKGVTALQQVCSFYTWKKDIKPREIEDAI 219

Query: 222 YHAAPGKLRVISVSEVSRAFHPNFSAKWRRYLYIFPLTDGGYKDQSSGSGEFFDTLRNNE 281
             AAPGKLRV+SV EVSRAFHPNFSAKWRRYLYIFPLTDG  KDQS G+GE  D+   NE
Sbjct: 220 NDAAPGKLRVVSVYEVSRAFHPNFSAKWRRYLYIFPLTDGQDKDQSGGNGESCDSFIYNE 279

Query: 282 IHDSSSKDDLENETKSYMFSVSKVNRLLQKLEGKLLSYKMFARDTKASRNEGPPTECFLY 341
           I DS SKD+LE E KSY+F V KVNRLLQKLEGK+LSYKMFARDTKASRN+GPPTECF++
Sbjct: 280 ICDSDSKDELEKENKSYVFGVRKVNRLLQKLEGKMLSYKMFARDTKASRNDGPPTECFVH 339

Query: 342 HARAIEARLPLADNDEETRVMCIELVANRFLRRMVRVLVATSIR 385
           HARA+EA+LP+ +N EETRVMCIELVANRFLR+MVRVLVAT+IR
Sbjct: 340 HARAMEAKLPITENGEETRVMCIELVANRFLRKMVRVLVATTIR 383


>Medtr8g101250.2 | tRNA pseudouridine synthase | HC |
           chr8:42499876-42506683 | 20130731
          Length = 355

 Score =  443 bits (1139), Expect = e-124,   Method: Compositional matrix adjust.
 Identities = 215/291 (73%), Positives = 242/291 (83%), Gaps = 5/291 (1%)

Query: 42  NYAHYNHTDPCKHARWNARESYEFMYARPWQRVNDFYLNAVLGNLSLPLLFGAEKIHDHN 101
           ++ HYNHT P K+ARWNARES+EFMYARPWQRVN FY N V GN SLPLLF ++ +  H+
Sbjct: 45  HHVHYNHTHPSKYARWNARESFEFMYARPWQRVNHFYSNTVRGNFSLPLLFQSQTLPVHD 104

Query: 102 NEPGIPAVSDKPEFESVASRGRGGRWARLTFKIVLSYHGGSFDGWQKQPGLNTVQSVIEG 161
           +   +P +S   EF+S AS+ R G+WAR  FKI+LSYHGGSFDGWQKQP LNTVQSV+E 
Sbjct: 105 SR--VPEIS---EFQSGASKDRSGKWARFNFKILLSYHGGSFDGWQKQPNLNTVQSVVEV 159

Query: 162 PLGTFVDENKTQQLKDRGLPIEGSVTVAGRTDKGVTALQQVCSFYTWKKDVKPRDIEDAI 221
            LG FVDENKTQ LKD+GLPI+G   VAGRTDKGVTALQQVCSFYTWKKD+KPR+IEDAI
Sbjct: 160 SLGKFVDENKTQLLKDKGLPIQGCAAVAGRTDKGVTALQQVCSFYTWKKDIKPREIEDAI 219

Query: 222 YHAAPGKLRVISVSEVSRAFHPNFSAKWRRYLYIFPLTDGGYKDQSSGSGEFFDTLRNNE 281
             AAPGKLRV+SV EVSRAFHPNFSAKWRRYLYIFPLTDG  KDQS G+GE  D+   NE
Sbjct: 220 NDAAPGKLRVVSVYEVSRAFHPNFSAKWRRYLYIFPLTDGQDKDQSGGNGESCDSFIYNE 279

Query: 282 IHDSSSKDDLENETKSYMFSVSKVNRLLQKLEGKLLSYKMFARDTKASRNE 332
           I DS SKD+LE E KSY+F V KVNRLLQKLEGK+LSYKMFARDTKASRN+
Sbjct: 280 ICDSDSKDELEKENKSYVFGVRKVNRLLQKLEGKMLSYKMFARDTKASRND 330


>Medtr4g131220.1 | tRNA pseudouridine synthase A, related protein |
           HC | chr4:54751590-54749272 | 20130731
          Length = 530

 Score = 62.8 bits (151), Expect = 6e-10,   Method: Compositional matrix adjust.
 Identities = 49/171 (28%), Positives = 76/171 (44%), Gaps = 25/171 (14%)

Query: 104 PGIP-AVSDKPEFESV-------------ASRGRGGRWARLTFKIVLSYHGGSFDGWQKQ 149
           P +P AV+++P  + V              + G   R+ R    I  +Y G  + G QK 
Sbjct: 14  PALPSAVAEEPATKKVKMSTTTSDDEGCTTAEGSKVRYKRRKVAIFFAYCGVGYQGMQKN 73

Query: 150 PGLNTVQSVIEGPLGTFVDENKTQQLKDRGLPIEGSVTVAGRTDKGVTALQQVCS--FYT 207
           PG  T++  +E  L  +V     +Q  DRGL        + RTDKGV+A+ QV S  FY 
Sbjct: 74  PGAKTIEGELEEAL--YVSGAVPEQ--DRGLSKRYDWARSARTDKGVSAVGQVVSGRFY- 128

Query: 208 WKKDVKPRDIEDAIYHAAPGKLRVISVSEVSRAFHPNFSAKWRRYLYIFPL 258
               + P    D +    P ++R+     V+ +F        RRY+Y+ P+
Sbjct: 129 ----IDPPGFVDRLNSNLPSQIRIFGFKRVTASFSAKKFCDRRRYVYLIPV 175


>Medtr4g131270.1 | tRNA pseudouridine synthase A, related protein |
           HC | chr4:54768312-54766231 | 20130731
          Length = 530

 Score = 62.4 bits (150), Expect = 6e-10,   Method: Compositional matrix adjust.
 Identities = 49/171 (28%), Positives = 76/171 (44%), Gaps = 25/171 (14%)

Query: 104 PGIP-AVSDKPEFESV-------------ASRGRGGRWARLTFKIVLSYHGGSFDGWQKQ 149
           P +P AV+++P  + V              + G   R+ R    I  +Y G  + G QK 
Sbjct: 14  PALPSAVAEEPATKKVKMSTTTSDDEGCTTAEGSKVRYKRRKVAIFFAYCGVGYQGMQKN 73

Query: 150 PGLNTVQSVIEGPLGTFVDENKTQQLKDRGLPIEGSVTVAGRTDKGVTALQQVCS--FYT 207
           PG  T++  +E  L  +V     +Q  DRGL        + RTDKGV+A+ QV S  FY 
Sbjct: 74  PGAKTIEGELEEAL--YVSGAVPEQ--DRGLSKRYDWARSARTDKGVSAVGQVVSGRFY- 128

Query: 208 WKKDVKPRDIEDAIYHAAPGKLRVISVSEVSRAFHPNFSAKWRRYLYIFPL 258
               + P    D +    P ++R+     V+ +F        RRY+Y+ P+
Sbjct: 129 ----IDPPGFVDRLNSNLPSQIRIFGFKRVTASFSAKKFCDRRRYVYLIPV 175


>Medtr4g123970.1 | tRNA pseudouridine synthase A | HC |
           chr4:51119303-51121602 | 20130731
          Length = 322

 Score = 55.1 bits (131), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 42/131 (32%), Positives = 58/131 (44%), Gaps = 15/131 (11%)

Query: 124 GGRWARLTFKIVLSYHGGSFDGWQKQPGLNTVQSVIEGPLGTFVDENKTQQLKDRGLPIE 183
           G +W     +++LSY G  + GWQ Q    TVQ  +E  L       +  +L+ + L + 
Sbjct: 41  GYKW-----RLLLSYDGTRYAGWQYQESPPTVQCAVEKAL------IRATKLQRKDLQLV 89

Query: 184 GSVTVAGRTDKGVTALQQVCSFYTWKKDVKPRDIEDAIYHAAPGKLRVISVSEVSRAFHP 243
           G    A RTD GV A  QV  F T         +  A+    P  +RV  +S  S  FH 
Sbjct: 90  G----ASRTDAGVHAWGQVAHFLTPFNYDNLDSVHAALNGLLPSDIRVREISPASAEFHA 145

Query: 244 NFSAKWRRYLY 254
            FS K + Y Y
Sbjct: 146 RFSVKSKIYHY 156