Miyakogusa Predicted Gene

Lj4g3v2468350.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2468350.2 Non Characterized Hit- tr|I3SEX4|I3SEX4_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,98.99,0,Transketolase, pyrimidine binding
domain,Transketolase-like, pyrimidine-binding domain; Thiamin
diph,CUFF.51053.2
         (400 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr3g076630.1 | pyruvate dehydrogenase E1 beta subunit | HC | ...   611   e-175
Medtr7g005380.2 | pyruvate dehydrogenase E1 beta subunit | HC | ...   238   1e-62
Medtr7g005380.1 | pyruvate dehydrogenase E1 beta subunit | HC | ...   238   1e-62
Medtr4g021470.1 | branched chain alpha-keto acid dehydrogenase E...   190   3e-48

>Medtr3g076630.1 | pyruvate dehydrogenase E1 beta subunit | HC |
           chr3:34442945-34446106 | 20130731
          Length = 403

 Score =  611 bits (1576), Expect = e-175,   Method: Compositional matrix adjust.
 Identities = 316/409 (77%), Positives = 329/409 (80%), Gaps = 15/409 (3%)

Query: 1   MATLFQGLGALTXXXXXXXXXXXXXXXXXXXXXKAGIFVVRS-DARANQT-----GARKH 54
           MATLFQ LGA                        + IFVVRS DA+ NQ      GA + 
Sbjct: 1   MATLFQALGA------ALTPFSPSTSFQSKERKCSSIFVVRSSDAKVNQVVLKSGGATRK 54

Query: 55  --DLLITNAVATK-EGSSVASTSKPGHXXXXXXXXXXXXXXXXXRDPNVCVMGEDVGHYG 111
              LLI NAVAT+   S  ++ SKPGH                 RDP VCVMGEDVGHYG
Sbjct: 55  AGQLLIPNAVATQGSSSVASAASKPGHELLLFEALREGLEEEMERDPCVCVMGEDVGHYG 114

Query: 112 GSYKVTKDLANKFGDLRVLDTPIAENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQIS 171
           GSYKVT++LA KFGDLRVLDTPIAENAFTGMGIGAAMTGLRP+IEGMNMGFLLLAFNQIS
Sbjct: 115 GSYKVTRNLAEKFGDLRVLDTPIAENAFTGMGIGAAMTGLRPIIEGMNMGFLLLAFNQIS 174

Query: 172 NNCGMLHYTSGGQFKXXXXXXXXXXXXXQLGAEHSQRLESYFQSIPGIQMVACSTPYNAK 231
           NNCGMLHYTSGGQFK             QLGAEHSQRLESYFQSIPGIQMVACSTPYNAK
Sbjct: 175 NNCGMLHYTSGGQFKIPVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAK 234

Query: 232 GLMKAAIRSDNPVILFEHVLLYNLKERIPDEEYVLSLEEAEMVRPGEHVTILTYSRMRYH 291
           GLMKAAIRSDNPVILFEHVLLYNLKERIPDEEYVLSLEEAEMVRPGEHVTILTYSRMRYH
Sbjct: 235 GLMKAAIRSDNPVILFEHVLLYNLKERIPDEEYVLSLEEAEMVRPGEHVTILTYSRMRYH 294

Query: 292 VMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAA 351
           VMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNS+KKTHRVLIVEECMRTGGIGASLTAA
Sbjct: 295 VMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSIKKTHRVLIVEECMRTGGIGASLTAA 354

Query: 352 ISENFNDYLDAPIVCLSSQDVPTPYAGPLEEMTVVQPAQIVTAVEQLCQ 400
           I+ENF+DYLDAPI+CLSSQDVPTPYAG LEE TVVQPAQIVTAVEQLCQ
Sbjct: 355 ITENFHDYLDAPIICLSSQDVPTPYAGTLEEWTVVQPAQIVTAVEQLCQ 403


>Medtr7g005380.2 | pyruvate dehydrogenase E1 beta subunit | HC |
           chr7:85481-92086 | 20130731
          Length = 361

 Score =  238 bits (606), Expect = 1e-62,   Method: Compositional matrix adjust.
 Identities = 124/308 (40%), Positives = 185/308 (60%), Gaps = 4/308 (1%)

Query: 97  DPNVCVMGEDVGHYGGSYKVTKDLANKFGDLRVLDTPIAENAFTGMGIGAAMTGLRPVIE 156
           DP V +MGE+VG Y G+YK++K L  K+G  RVLDTPI E  FTG+G+GAA  GL+PV+E
Sbjct: 45  DPKVFLMGEEVGEYQGAYKISKGLLEKYGPERVLDTPITEAGFTGIGVGAAYYGLKPVVE 104

Query: 157 GMNMGFLLLAFNQISNNCGMLHYTSGGQFKXXXXXXXXXXXXXQLGAEHSQRLESYFQSI 216
            M   F + A + I N+    +Y S GQ                +GA+HS    S++ S 
Sbjct: 105 FMTFNFSMQAIDHIINSAAKSNYMSAGQINVPIVFRGPNGAAAGVGAQHSHCYASWYGSC 164

Query: 217 PGIQMVACSTPYNAKGLMKAAIRSDNPVILFEHVLLYN----LKERIPDEEYVLSLEEAE 272
           PG++++A  +  +A+GL+KAAIR  +PV+  E+ LLY     +   + D  + L + +A+
Sbjct: 165 PGLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFCLPIGKAK 224

Query: 273 MVRPGEHVTILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRV 332
           + R G+ VTI  +S+M    ++AA+TL  +G   EVI++RS++P D  TI  SV+KT+R+
Sbjct: 225 IEREGKDVTITAFSKMVGFALKAAETLEKEGISAEVINLRSIRPLDRATINASVRKTNRL 284

Query: 333 LIVEECMRTGGIGASLTAAISENFNDYLDAPIVCLSSQDVPTPYAGPLEEMTVVQPAQIV 392
           + VEE     G+GA + A++ E    YLDAP+  ++  DVP PYA  LE + V Q   IV
Sbjct: 285 VTVEEGFPQHGVGAEICASVIEESFGYLDAPVERIAGADVPMPYAANLERLAVPQIEDIV 344

Query: 393 TAVEQLCQ 400
            A ++ C 
Sbjct: 345 RAAKRACH 352


>Medtr7g005380.1 | pyruvate dehydrogenase E1 beta subunit | HC |
           chr7:86084-92086 | 20130731
          Length = 361

 Score =  238 bits (606), Expect = 1e-62,   Method: Compositional matrix adjust.
 Identities = 124/308 (40%), Positives = 185/308 (60%), Gaps = 4/308 (1%)

Query: 97  DPNVCVMGEDVGHYGGSYKVTKDLANKFGDLRVLDTPIAENAFTGMGIGAAMTGLRPVIE 156
           DP V +MGE+VG Y G+YK++K L  K+G  RVLDTPI E  FTG+G+GAA  GL+PV+E
Sbjct: 45  DPKVFLMGEEVGEYQGAYKISKGLLEKYGPERVLDTPITEAGFTGIGVGAAYYGLKPVVE 104

Query: 157 GMNMGFLLLAFNQISNNCGMLHYTSGGQFKXXXXXXXXXXXXXQLGAEHSQRLESYFQSI 216
            M   F + A + I N+    +Y S GQ                +GA+HS    S++ S 
Sbjct: 105 FMTFNFSMQAIDHIINSAAKSNYMSAGQINVPIVFRGPNGAAAGVGAQHSHCYASWYGSC 164

Query: 217 PGIQMVACSTPYNAKGLMKAAIRSDNPVILFEHVLLYN----LKERIPDEEYVLSLEEAE 272
           PG++++A  +  +A+GL+KAAIR  +PV+  E+ LLY     +   + D  + L + +A+
Sbjct: 165 PGLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFCLPIGKAK 224

Query: 273 MVRPGEHVTILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRV 332
           + R G+ VTI  +S+M    ++AA+TL  +G   EVI++RS++P D  TI  SV+KT+R+
Sbjct: 225 IEREGKDVTITAFSKMVGFALKAAETLEKEGISAEVINLRSIRPLDRATINASVRKTNRL 284

Query: 333 LIVEECMRTGGIGASLTAAISENFNDYLDAPIVCLSSQDVPTPYAGPLEEMTVVQPAQIV 392
           + VEE     G+GA + A++ E    YLDAP+  ++  DVP PYA  LE + V Q   IV
Sbjct: 285 VTVEEGFPQHGVGAEICASVIEESFGYLDAPVERIAGADVPMPYAANLERLAVPQIEDIV 344

Query: 393 TAVEQLCQ 400
            A ++ C 
Sbjct: 345 RAAKRACH 352


>Medtr4g021470.1 | branched chain alpha-keto acid dehydrogenase E1
           beta subunit | HC | chr4:6990880-6984927 | 20130731
          Length = 358

 Score =  190 bits (482), Expect = 3e-48,   Method: Compositional matrix adjust.
 Identities = 106/281 (37%), Positives = 152/281 (54%), Gaps = 3/281 (1%)

Query: 97  DPNVCVMGEDVGHYGGSYKVTKDLANKFGDLRVLDTPIAENAFTGMGIGAAMTGLRPVIE 156
           DP   V GEDVG +GG ++ T  LA++FG  RV +TP+ E    G GIG A  G R + E
Sbjct: 55  DPRAYVFGEDVG-FGGVFRCTTGLADRFGKKRVFNTPLCEQGIVGFGIGLAAMGNRAIAE 113

Query: 157 GMNMGFLLLAFNQISNNCGMLHYTSGGQFKXXXXXXXXXXXXXQLGAE-HSQRLESYFQS 215
                ++  AF+QI N      Y SG QF                G   HSQ  E++F  
Sbjct: 114 IQFADYIFPAFDQIVNEAAKFRYRSGNQFNCGGLTIRTPYGAVGHGGHYHSQSPEAFFCH 173

Query: 216 IPGIQMVACSTPYNAKGLMKAAIRSDNPVILFEHVLLYNLK-ERIPDEEYVLSLEEAEMV 274
           +PGI++V   +P  AKGL+ + IR  NP++ FE   LY L  E +P+ +Y+L L EAE++
Sbjct: 174 VPGIKVVIPRSPKEAKGLLLSCIRDPNPIVFFEPKWLYRLAVEEVPEGDYMLPLSEAEVI 233

Query: 275 RPGEHVTILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLI 334
           R G  +T++ +      + QA      +G   E+ID+++L P+D  T+  SVKKT R+LI
Sbjct: 234 REGSDITLVGWGAQLSIMEQACVDAEKEGISCELIDLKTLIPWDKETVEASVKKTGRLLI 293

Query: 335 VEECMRTGGIGASLTAAISENFNDYLDAPIVCLSSQDVPTP 375
             E   TGG GA ++A+I E     L+AP+  +   D P P
Sbjct: 294 SHEAPVTGGFGAEISASILERCFSRLEAPVARVCGLDTPFP 334