Miyakogusa Predicted Gene

Lj4g3v2020600.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2020600.1 Non Characterized Hit- tr|I1JGU1|I1JGU1_SOYBN
Uncharacterized protein OS=Glycine max PE=4 SV=1,80.4,0,P-loop
containing nucleoside triphosphate hydrolases,NULL;
AFG1_ATPase,ATPase, AFG1-like; no descrip,CUFF.50204.1
         (502 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g129950.2 | AFG1-family ATPase | HC | chr4:54196842-541918...   826   0.0  
Medtr4g129950.1 | AFG1-family ATPase | HC | chr4:54196842-541918...   611   e-175
Medtr4g129950.3 | AFG1-family ATPase | HC | chr4:54196842-541917...   611   e-175
Medtr1g012370.4 | AFG1-family ATPase | HC | chr1:2443925-2434441...   174   2e-43
Medtr1g012370.2 | AFG1-family ATPase | HC | chr1:2443925-2434182...   174   2e-43
Medtr1g012370.1 | AFG1-family ATPase | HC | chr1:2443929-2434267...   174   2e-43
Medtr1g012370.3 | AFG1-family ATPase | HC | chr1:2443929-2434182...   174   2e-43

>Medtr4g129950.2 | AFG1-family ATPase | HC | chr4:54196842-54191819
           | 20130731
          Length = 500

 Score =  826 bits (2134), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 401/505 (79%), Positives = 444/505 (87%), Gaps = 8/505 (1%)

Query: 1   MRRVVRPVRQLAQASQRHQKCILITDPVAETPFHFEKSSHLHSSAYRVLDHHVETTLAKN 60
           MR  VR +RQL    QR Q C++I + VAE P +FEK   +H+SAYRVLD   E+ + KN
Sbjct: 1   MRISVRSLRQLRHVYQR-QSCVMINNLVAEKPCYFEKCCQIHNSAYRVLDQ-AESNIFKN 58

Query: 61  SSF---TTRGLSIDAAKLTHGEIERAGPLVEYERRIATGELVDGDNFQLYTLTELQRLYD 117
             F   +TR +S DA K+T+G  +++GPLVEYERRIA GELVDGD+ Q+ TLTELQRLYD
Sbjct: 59  HPFINTSTRSISADAVKITNGVTKKSGPLVEYERRIANGELVDGDSCQVETLTELQRLYD 118

Query: 118 ELVASADTCQLDRDAAEKPVRSGWLWSRLLYHPSHSPVKGLYLYGGVGTGKTMLMDLFFN 177
           ELV  AD CQLDR++ EKPVR GWLWSRLL HPSHSPVKGLYLYGGVGTGKTMLMDLF++
Sbjct: 119 ELVEHADECQLDRNS-EKPVRHGWLWSRLLSHPSHSPVKGLYLYGGVGTGKTMLMDLFYD 177

Query: 178 QLPSNWRKKRIHFHDFMLNVHSLLQKHMGLSDPLEVVAEEISDEAILLCLDEFMVTDVAD 237
           QLPSNW+KKRIHFHDFMLNVHSLLQKH GLSDPL+VVA EISDEA+LLCLDEFMVTDVAD
Sbjct: 178 QLPSNWKKKRIHFHDFMLNVHSLLQKHKGLSDPLDVVAGEISDEAVLLCLDEFMVTDVAD 237

Query: 238 ALLLNRLFKHLFSKGAILVATSNRVPDNLYEGGLQRDLFLPFIATLKERCVVHEIGSSID 297
           AL+LNRLF+HLFSKG ILV+TSNR PDNLYEGGLQRDLFLPFIATLKERC+ HEIGSS D
Sbjct: 238 ALILNRLFRHLFSKGIILVSTSNRAPDNLYEGGLQRDLFLPFIATLKERCIAHEIGSSTD 297

Query: 298 YRKMTSGEQGFYLVGSGLSDFLKKRFQDLIGEGTPTPQVVEVVMGRTLHVPLGANGCAYF 357
           YRKMTSG QGFYLVGS  S FLKK+FQ LIGEGTPTPQ VEVVMGR LHVPLGANGCAYF
Sbjct: 298 YRKMTSGGQGFYLVGSDSSGFLKKKFQQLIGEGTPTPQEVEVVMGRKLHVPLGANGCAYF 357

Query: 358 PFEELCDKPLGAADYFGLFKNFHTLALDGIPVFGVSNKSAAHRFVTLIDVMYENRARLLC 417
           PFEELCDKPLGAADYFGLFK FHTLALDGIP+FG+SNK+AAHRFVTL+DV+YEN+ARLLC
Sbjct: 358 PFEELCDKPLGAADYFGLFKKFHTLALDGIPIFGLSNKAAAHRFVTLVDVIYENKARLLC 417

Query: 418 TADGSPQDLFEKVVTVSEAKQMAPRTSSRSRKNDVIDLCVDNELGFAKDRTISRLTELNS 477
           +ADGSP DLF+K+VT+SEAKQ APRTSSRSRKND  DLCVDNELGFAKDRTISRLTE+NS
Sbjct: 418 SADGSPLDLFQKIVTISEAKQRAPRTSSRSRKNDEADLCVDNELGFAKDRTISRLTEINS 477

Query: 478 KEYLEHHAATLAEKKERVDQNVLEA 502
           +EYLEHHA  LAEKKER DQNV+EA
Sbjct: 478 REYLEHHA--LAEKKEREDQNVVEA 500


>Medtr4g129950.1 | AFG1-family ATPase | HC | chr4:54196842-54191869
           | 20130731
          Length = 400

 Score =  611 bits (1576), Expect = e-175,   Method: Compositional matrix adjust.
 Identities = 296/384 (77%), Positives = 327/384 (85%), Gaps = 6/384 (1%)

Query: 1   MRRVVRPVRQLAQASQRHQKCILITDPVAETPFHFEKSSHLHSSAYRVLDHHVETTLAKN 60
           MR  VR +RQL    QR Q C++I + VAE P +FEK   +H+SAYRVLD   E+ + KN
Sbjct: 1   MRISVRSLRQLRHVYQR-QSCVMINNLVAEKPCYFEKCCQIHNSAYRVLDQ-AESNIFKN 58

Query: 61  SSF---TTRGLSIDAAKLTHGEIERAGPLVEYERRIATGELVDGDNFQLYTLTELQRLYD 117
             F   +TR +S DA K+T+G  +++GPLVEYERRIA GELVDGD+ Q+ TLTELQRLYD
Sbjct: 59  HPFINTSTRSISADAVKITNGVTKKSGPLVEYERRIANGELVDGDSCQVETLTELQRLYD 118

Query: 118 ELVASADTCQLDRDAAEKPVRSGWLWSRLLYHPSHSPVKGLYLYGGVGTGKTMLMDLFFN 177
           ELV  AD CQLDR++ EKPVR GWLWSRLL HPSHSPVKGLYLYGGVGTGKTMLMDLF++
Sbjct: 119 ELVEHADECQLDRNS-EKPVRHGWLWSRLLSHPSHSPVKGLYLYGGVGTGKTMLMDLFYD 177

Query: 178 QLPSNWRKKRIHFHDFMLNVHSLLQKHMGLSDPLEVVAEEISDEAILLCLDEFMVTDVAD 237
           QLPSNW+KKRIHFHDFMLNVHSLLQKH GLSDPL+VVA EISDEA+LLCLDEFMVTDVAD
Sbjct: 178 QLPSNWKKKRIHFHDFMLNVHSLLQKHKGLSDPLDVVAGEISDEAVLLCLDEFMVTDVAD 237

Query: 238 ALLLNRLFKHLFSKGAILVATSNRVPDNLYEGGLQRDLFLPFIATLKERCVVHEIGSSID 297
           AL+LNRLF+HLFSKG ILV+TSNR PDNLYEGGLQRDLFLPFIATLKERC+ HEIGSS D
Sbjct: 238 ALILNRLFRHLFSKGIILVSTSNRAPDNLYEGGLQRDLFLPFIATLKERCIAHEIGSSTD 297

Query: 298 YRKMTSGEQGFYLVGSGLSDFLKKRFQDLIGEGTPTPQVVEVVMGRTLHVPLGANGCAYF 357
           YRKMTSG QGFYLVGS  S FLKK+FQ LIGEGTPTPQ VEVVMGR LHVPLGANGCAYF
Sbjct: 298 YRKMTSGGQGFYLVGSDSSGFLKKKFQQLIGEGTPTPQEVEVVMGRKLHVPLGANGCAYF 357

Query: 358 PFEELCDKPLGAADYFGLFKNFHT 381
           PFEELCDKPLGAADYFGLF   + 
Sbjct: 358 PFEELCDKPLGAADYFGLFSKLYV 381


>Medtr4g129950.3 | AFG1-family ATPase | HC | chr4:54196842-54191756
           | 20130731
          Length = 400

 Score =  611 bits (1576), Expect = e-175,   Method: Compositional matrix adjust.
 Identities = 296/384 (77%), Positives = 327/384 (85%), Gaps = 6/384 (1%)

Query: 1   MRRVVRPVRQLAQASQRHQKCILITDPVAETPFHFEKSSHLHSSAYRVLDHHVETTLAKN 60
           MR  VR +RQL    QR Q C++I + VAE P +FEK   +H+SAYRVLD   E+ + KN
Sbjct: 1   MRISVRSLRQLRHVYQR-QSCVMINNLVAEKPCYFEKCCQIHNSAYRVLDQ-AESNIFKN 58

Query: 61  SSF---TTRGLSIDAAKLTHGEIERAGPLVEYERRIATGELVDGDNFQLYTLTELQRLYD 117
             F   +TR +S DA K+T+G  +++GPLVEYERRIA GELVDGD+ Q+ TLTELQRLYD
Sbjct: 59  HPFINTSTRSISADAVKITNGVTKKSGPLVEYERRIANGELVDGDSCQVETLTELQRLYD 118

Query: 118 ELVASADTCQLDRDAAEKPVRSGWLWSRLLYHPSHSPVKGLYLYGGVGTGKTMLMDLFFN 177
           ELV  AD CQLDR++ EKPVR GWLWSRLL HPSHSPVKGLYLYGGVGTGKTMLMDLF++
Sbjct: 119 ELVEHADECQLDRNS-EKPVRHGWLWSRLLSHPSHSPVKGLYLYGGVGTGKTMLMDLFYD 177

Query: 178 QLPSNWRKKRIHFHDFMLNVHSLLQKHMGLSDPLEVVAEEISDEAILLCLDEFMVTDVAD 237
           QLPSNW+KKRIHFHDFMLNVHSLLQKH GLSDPL+VVA EISDEA+LLCLDEFMVTDVAD
Sbjct: 178 QLPSNWKKKRIHFHDFMLNVHSLLQKHKGLSDPLDVVAGEISDEAVLLCLDEFMVTDVAD 237

Query: 238 ALLLNRLFKHLFSKGAILVATSNRVPDNLYEGGLQRDLFLPFIATLKERCVVHEIGSSID 297
           AL+LNRLF+HLFSKG ILV+TSNR PDNLYEGGLQRDLFLPFIATLKERC+ HEIGSS D
Sbjct: 238 ALILNRLFRHLFSKGIILVSTSNRAPDNLYEGGLQRDLFLPFIATLKERCIAHEIGSSTD 297

Query: 298 YRKMTSGEQGFYLVGSGLSDFLKKRFQDLIGEGTPTPQVVEVVMGRTLHVPLGANGCAYF 357
           YRKMTSG QGFYLVGS  S FLKK+FQ LIGEGTPTPQ VEVVMGR LHVPLGANGCAYF
Sbjct: 298 YRKMTSGGQGFYLVGSDSSGFLKKKFQQLIGEGTPTPQEVEVVMGRKLHVPLGANGCAYF 357

Query: 358 PFEELCDKPLGAADYFGLFKNFHT 381
           PFEELCDKPLGAADYFGLF   + 
Sbjct: 358 PFEELCDKPLGAADYFGLFSKLYV 381


>Medtr1g012370.4 | AFG1-family ATPase | HC | chr1:2443925-2434441 |
           20130731
          Length = 520

 Score =  174 bits (442), Expect = 2e-43,   Method: Compositional matrix adjust.
 Identities = 122/408 (29%), Positives = 187/408 (45%), Gaps = 75/408 (18%)

Query: 150 PSHSPV-KGLYLYGGVGTGKTMLMDLFFNQLPSNWR-KKRIHFHDFMLNV----HSLLQK 203
           P+  P  KGLY+YG VG+GKTMLMD+F++      + ++R HFH+ ML +    H   +K
Sbjct: 83  PTAPPAPKGLYIYGNVGSGKTMLMDMFYSATEGIVKHRRRYHFHEAMLRINEHMHKTWKK 142

Query: 204 HM-------GLSD-----PLEVVAEE--------------------ISDE---------- 221
            M       G+S      P +  A+E                    ++D+          
Sbjct: 143 QMEEKPLQSGISSWIMNLPFDTKAKEWLAAEERYKKEVQMKHILPDVADKFFLDREGEEK 202

Query: 222 -AILLCLDEFMVTDVADALLLNRLFKHLFSKGAILVATSNRVPDNLYEGGLQRDLFLPFI 280
            A +LC DE    DV   + L+ +   L S G I+VATSNR P +L E  +  + F   +
Sbjct: 203 GANILCFDEIQTVDVFAIVALSGILSRLLSSGTIIVATSNRAPKDLNEANMVPEFFQNLL 262

Query: 281 ATLKERCVVHEIGSSIDYRKMTSGEQG-----FYLVGSGLSDFLKKRFQDLIGE--GTPT 333
           + L+E C    +GS IDYR+  +          + +     +  +K++QD  G   G   
Sbjct: 263 SNLEEHCEKVLVGSEIDYRRFIAQRSENRVNYLWPIERETINKFEKKWQDATGRFGGKVI 322

Query: 334 PQVVEVVMGRTLHVPLGANGCAYFPFEELCDKPLGAADYFGLFKNFHTLALDGIPVFGVS 393
              + V+ GRTL VP    G A F F+ LC +PLGAADY  + +N+HT+ +  IP+  + 
Sbjct: 323 SNTISVMFGRTLEVPESCEGVARFTFDYLCGRPLGAADYIAVAENYHTVFISDIPMMSMR 382

Query: 394 NKSAAHRFVTLIDVMYENRARLLCTADGSPQDLF----EKVVTVSEAKQMAPRTSSRSRK 449
            +  A RF+TLID +Y + + L C A  S  +LF    E  +   E+ Q          +
Sbjct: 383 IRDKARRFITLIDELYNHHSCLCCLASSSIDELFQGTEEGTLFDLESFQFETEAEGSKLR 442

Query: 450 NDVI---------------DLCVDNELGFAKDRTISRLTELNSKEYLE 482
            DV+                +    E  F   R +SRL E+ ++ YL+
Sbjct: 443 RDVLAEGNVGSGGTPVGITSILSGQEELFTFQRAVSRLIEMQTQLYLD 490


>Medtr1g012370.2 | AFG1-family ATPase | HC | chr1:2443925-2434182 |
           20130731
          Length = 497

 Score =  174 bits (441), Expect = 2e-43,   Method: Compositional matrix adjust.
 Identities = 122/408 (29%), Positives = 187/408 (45%), Gaps = 75/408 (18%)

Query: 150 PSHSPV-KGLYLYGGVGTGKTMLMDLFFNQLPSNWR-KKRIHFHDFMLNV----HSLLQK 203
           P+  P  KGLY+YG VG+GKTMLMD+F++      + ++R HFH+ ML +    H   +K
Sbjct: 60  PTAPPAPKGLYIYGNVGSGKTMLMDMFYSATEGIVKHRRRYHFHEAMLRINEHMHKTWKK 119

Query: 204 HM-------GLSD-----PLEVVAEE--------------------ISDE---------- 221
            M       G+S      P +  A+E                    ++D+          
Sbjct: 120 QMEEKPLQSGISSWIMNLPFDTKAKEWLAAEERYKKEVQMKHILPDVADKFFLDREGEEK 179

Query: 222 -AILLCLDEFMVTDVADALLLNRLFKHLFSKGAILVATSNRVPDNLYEGGLQRDLFLPFI 280
            A +LC DE    DV   + L+ +   L S G I+VATSNR P +L E  +  + F   +
Sbjct: 180 GANILCFDEIQTVDVFAIVALSGILSRLLSSGTIIVATSNRAPKDLNEANMVPEFFQNLL 239

Query: 281 ATLKERCVVHEIGSSIDYRKMTSGEQG-----FYLVGSGLSDFLKKRFQDLIGE--GTPT 333
           + L+E C    +GS IDYR+  +          + +     +  +K++QD  G   G   
Sbjct: 240 SNLEEHCEKVLVGSEIDYRRFIAQRSENRVNYLWPIERETINKFEKKWQDATGRFGGKVI 299

Query: 334 PQVVEVVMGRTLHVPLGANGCAYFPFEELCDKPLGAADYFGLFKNFHTLALDGIPVFGVS 393
              + V+ GRTL VP    G A F F+ LC +PLGAADY  + +N+HT+ +  IP+  + 
Sbjct: 300 SNTISVMFGRTLEVPESCEGVARFTFDYLCGRPLGAADYIAVAENYHTVFISDIPMMSMR 359

Query: 394 NKSAAHRFVTLIDVMYENRARLLCTADGSPQDLF----EKVVTVSEAKQMAPRTSSRSRK 449
            +  A RF+TLID +Y + + L C A  S  +LF    E  +   E+ Q          +
Sbjct: 360 IRDKARRFITLIDELYNHHSCLCCLASSSIDELFQGTEEGTLFDLESFQFETEAEGSKLR 419

Query: 450 NDVI---------------DLCVDNELGFAKDRTISRLTELNSKEYLE 482
            DV+                +    E  F   R +SRL E+ ++ YL+
Sbjct: 420 RDVLAEGNVGSGGTPVGITSILSGQEELFTFQRAVSRLIEMQTQLYLD 467


>Medtr1g012370.1 | AFG1-family ATPase | HC | chr1:2443929-2434267 |
           20130731
          Length = 621

 Score =  174 bits (441), Expect = 2e-43,   Method: Compositional matrix adjust.
 Identities = 122/408 (29%), Positives = 187/408 (45%), Gaps = 75/408 (18%)

Query: 150 PSHSPV-KGLYLYGGVGTGKTMLMDLFFNQLPSNWR-KKRIHFHDFMLNV----HSLLQK 203
           P+  P  KGLY+YG VG+GKTMLMD+F++      + ++R HFH+ ML +    H   +K
Sbjct: 184 PTAPPAPKGLYIYGNVGSGKTMLMDMFYSATEGIVKHRRRYHFHEAMLRINEHMHKTWKK 243

Query: 204 HM-------GLSD-----PLEVVAEE--------------------ISDE---------- 221
            M       G+S      P +  A+E                    ++D+          
Sbjct: 244 QMEEKPLQSGISSWIMNLPFDTKAKEWLAAEERYKKEVQMKHILPDVADKFFLDREGEEK 303

Query: 222 -AILLCLDEFMVTDVADALLLNRLFKHLFSKGAILVATSNRVPDNLYEGGLQRDLFLPFI 280
            A +LC DE    DV   + L+ +   L S G I+VATSNR P +L E  +  + F   +
Sbjct: 304 GANILCFDEIQTVDVFAIVALSGILSRLLSSGTIIVATSNRAPKDLNEANMVPEFFQNLL 363

Query: 281 ATLKERCVVHEIGSSIDYRKMTSGEQG-----FYLVGSGLSDFLKKRFQDLIGE--GTPT 333
           + L+E C    +GS IDYR+  +          + +     +  +K++QD  G   G   
Sbjct: 364 SNLEEHCEKVLVGSEIDYRRFIAQRSENRVNYLWPIERETINKFEKKWQDATGRFGGKVI 423

Query: 334 PQVVEVVMGRTLHVPLGANGCAYFPFEELCDKPLGAADYFGLFKNFHTLALDGIPVFGVS 393
              + V+ GRTL VP    G A F F+ LC +PLGAADY  + +N+HT+ +  IP+  + 
Sbjct: 424 SNTISVMFGRTLEVPESCEGVARFTFDYLCGRPLGAADYIAVAENYHTVFISDIPMMSMR 483

Query: 394 NKSAAHRFVTLIDVMYENRARLLCTADGSPQDLF----EKVVTVSEAKQMAPRTSSRSRK 449
            +  A RF+TLID +Y + + L C A  S  +LF    E  +   E+ Q          +
Sbjct: 484 IRDKARRFITLIDELYNHHSCLCCLASSSIDELFQGTEEGTLFDLESFQFETEAEGSKLR 543

Query: 450 NDVI---------------DLCVDNELGFAKDRTISRLTELNSKEYLE 482
            DV+                +    E  F   R +SRL E+ ++ YL+
Sbjct: 544 RDVLAEGNVGSGGTPVGITSILSGQEELFTFQRAVSRLIEMQTQLYLD 591


>Medtr1g012370.3 | AFG1-family ATPase | HC | chr1:2443929-2434182 |
           20130731
          Length = 621

 Score =  174 bits (441), Expect = 2e-43,   Method: Compositional matrix adjust.
 Identities = 122/408 (29%), Positives = 187/408 (45%), Gaps = 75/408 (18%)

Query: 150 PSHSPV-KGLYLYGGVGTGKTMLMDLFFNQLPSNWR-KKRIHFHDFMLNV----HSLLQK 203
           P+  P  KGLY+YG VG+GKTMLMD+F++      + ++R HFH+ ML +    H   +K
Sbjct: 184 PTAPPAPKGLYIYGNVGSGKTMLMDMFYSATEGIVKHRRRYHFHEAMLRINEHMHKTWKK 243

Query: 204 HM-------GLSD-----PLEVVAEE--------------------ISDE---------- 221
            M       G+S      P +  A+E                    ++D+          
Sbjct: 244 QMEEKPLQSGISSWIMNLPFDTKAKEWLAAEERYKKEVQMKHILPDVADKFFLDREGEEK 303

Query: 222 -AILLCLDEFMVTDVADALLLNRLFKHLFSKGAILVATSNRVPDNLYEGGLQRDLFLPFI 280
            A +LC DE    DV   + L+ +   L S G I+VATSNR P +L E  +  + F   +
Sbjct: 304 GANILCFDEIQTVDVFAIVALSGILSRLLSSGTIIVATSNRAPKDLNEANMVPEFFQNLL 363

Query: 281 ATLKERCVVHEIGSSIDYRKMTSGEQG-----FYLVGSGLSDFLKKRFQDLIGE--GTPT 333
           + L+E C    +GS IDYR+  +          + +     +  +K++QD  G   G   
Sbjct: 364 SNLEEHCEKVLVGSEIDYRRFIAQRSENRVNYLWPIERETINKFEKKWQDATGRFGGKVI 423

Query: 334 PQVVEVVMGRTLHVPLGANGCAYFPFEELCDKPLGAADYFGLFKNFHTLALDGIPVFGVS 393
              + V+ GRTL VP    G A F F+ LC +PLGAADY  + +N+HT+ +  IP+  + 
Sbjct: 424 SNTISVMFGRTLEVPESCEGVARFTFDYLCGRPLGAADYIAVAENYHTVFISDIPMMSMR 483

Query: 394 NKSAAHRFVTLIDVMYENRARLLCTADGSPQDLF----EKVVTVSEAKQMAPRTSSRSRK 449
            +  A RF+TLID +Y + + L C A  S  +LF    E  +   E+ Q          +
Sbjct: 484 IRDKARRFITLIDELYNHHSCLCCLASSSIDELFQGTEEGTLFDLESFQFETEAEGSKLR 543

Query: 450 NDVI---------------DLCVDNELGFAKDRTISRLTELNSKEYLE 482
            DV+                +    E  F   R +SRL E+ ++ YL+
Sbjct: 544 RDVLAEGNVGSGGTPVGITSILSGQEELFTFQRAVSRLIEMQTQLYLD 591