Miyakogusa Predicted Gene
- Lj4g3v1983210.2
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj4g3v1983210.2 Non Characterized Hit- tr|I1MSK7|I1MSK7_SOYBN
Uncharacterized protein OS=Glycine max PE=4
SV=1,75.69,0,GLYHDRLASE47,Glycoside hydrolase, family 47;
Seven-hairpin glycosidases,Glycoside hydrolase, family ,CUFF.50025.2
(340 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr4g127310.1 | mannosyl-oligosaccharide 1,2-alpha-mannosidase... 540 e-154
Medtr4g127310.2 | mannosyl-oligosaccharide 1,2-alpha-mannosidase... 540 e-154
Medtr4g008140.1 | mannosyl-oligosaccharide 1,2-alpha-mannosidase... 439 e-123
Medtr2g019520.1 | mannosyl-oligosaccharide 1,2-alpha-mannosidase... 196 3e-50
Medtr1g076280.1 | glycosyl hydrolase family 47 protein | HC | ch... 152 6e-37
Medtr1g076280.2 | glycosyl hydrolase family 47 protein | HC | ch... 151 8e-37
Medtr4g106370.1 | glycosyl hydrolase family 47 protein | HC | ch... 124 1e-28
>Medtr4g127310.1 | mannosyl-oligosaccharide 1,2-alpha-mannosidase |
HC | chr4:52832404-52824659 | 20130731
Length = 584
Score = 540 bits (1391), Expect = e-154, Method: Compositional matrix adjust.
Identities = 264/345 (76%), Positives = 302/345 (87%), Gaps = 8/345 (2%)
Query: 1 MGKKSYSSSSTWRGWRYVQPRYYIKRPKRLALIIIVCIALTWLVYDRQLLNKEHQEDILR 60
M +K+YSSSS+ RYVQPRYY KRPKR A +I + I+L W VYDRQLL++EHQ+DI R
Sbjct: 1 MARKNYSSSSS--ALRYVQPRYYAKRPKRFAFLIFLLISLAWFVYDRQLLSREHQQDIFR 58
Query: 61 LQEEVTGLQNTLEEIKGQMKGSEAESLQNDEKMNDLTNVVSVE-----DDPISIHRREKV 115
L++EVT LQ TLE+IKG +KGSE ES++ DEK+N +V E +DPIS+ RREKV
Sbjct: 59 LKQEVTRLQKTLEDIKGNIKGSE-ESIEIDEKINKGVSVADEEEEEEENDPISLERREKV 117
Query: 116 KDAMLHAWTAYEKYAWGMDELKPQSMNGVDSFGGLGATLVDSLDTLFIMGLDSQFRRASE 175
KDAMLHAWT+YEKYAWG DELKP SM GVDSFGGLGATLVDSLDTLFIMGLD+QF+RA E
Sbjct: 118 KDAMLHAWTSYEKYAWGKDELKPPSMTGVDSFGGLGATLVDSLDTLFIMGLDTQFKRARE 177
Query: 176 WIAESLHFNKNIEVSVFETTIRVLGGLLSAYDLSGDKVFLEKARDIADKLLPAWDTPSGI 235
WI +SL+FNK++EVSVFETTIRVLGGLLSAYDLSGD+VFLEKARD+AD+LLPAW+TPSGI
Sbjct: 178 WIEKSLYFNKDLEVSVFETTIRVLGGLLSAYDLSGDEVFLEKARDLADRLLPAWNTPSGI 237
Query: 236 PYNRINLENGNTNNPRWTRGNSILADSGSEQLEFIALSQRTNDPKYQQRAEKVIKQFRKI 295
PYNRINL GNT+NP+WTRGNSILADSGSEQLEFIALSQRT DPKYQ++AEKVIK+ RK
Sbjct: 238 PYNRINLAYGNTDNPKWTRGNSILADSGSEQLEFIALSQRTKDPKYQEKAEKVIKELRKR 297
Query: 296 FPEDGLLPIYIDPKTGTKPSGAITFGAMGDSFYEYLLKAWVQGNK 340
FPEDGLLPIYI+P TG K +GAITFGAMGDSFYEYLLKAW+QGNK
Sbjct: 298 FPEDGLLPIYINPLTGDKSAGAITFGAMGDSFYEYLLKAWIQGNK 342
>Medtr4g127310.2 | mannosyl-oligosaccharide 1,2-alpha-mannosidase |
HC | chr4:52832404-52824659 | 20130731
Length = 584
Score = 540 bits (1391), Expect = e-154, Method: Compositional matrix adjust.
Identities = 264/345 (76%), Positives = 302/345 (87%), Gaps = 8/345 (2%)
Query: 1 MGKKSYSSSSTWRGWRYVQPRYYIKRPKRLALIIIVCIALTWLVYDRQLLNKEHQEDILR 60
M +K+YSSSS+ RYVQPRYY KRPKR A +I + I+L W VYDRQLL++EHQ+DI R
Sbjct: 1 MARKNYSSSSS--ALRYVQPRYYAKRPKRFAFLIFLLISLAWFVYDRQLLSREHQQDIFR 58
Query: 61 LQEEVTGLQNTLEEIKGQMKGSEAESLQNDEKMNDLTNVVSVE-----DDPISIHRREKV 115
L++EVT LQ TLE+IKG +KGSE ES++ DEK+N +V E +DPIS+ RREKV
Sbjct: 59 LKQEVTRLQKTLEDIKGNIKGSE-ESIEIDEKINKGVSVADEEEEEEENDPISLERREKV 117
Query: 116 KDAMLHAWTAYEKYAWGMDELKPQSMNGVDSFGGLGATLVDSLDTLFIMGLDSQFRRASE 175
KDAMLHAWT+YEKYAWG DELKP SM GVDSFGGLGATLVDSLDTLFIMGLD+QF+RA E
Sbjct: 118 KDAMLHAWTSYEKYAWGKDELKPPSMTGVDSFGGLGATLVDSLDTLFIMGLDTQFKRARE 177
Query: 176 WIAESLHFNKNIEVSVFETTIRVLGGLLSAYDLSGDKVFLEKARDIADKLLPAWDTPSGI 235
WI +SL+FNK++EVSVFETTIRVLGGLLSAYDLSGD+VFLEKARD+AD+LLPAW+TPSGI
Sbjct: 178 WIEKSLYFNKDLEVSVFETTIRVLGGLLSAYDLSGDEVFLEKARDLADRLLPAWNTPSGI 237
Query: 236 PYNRINLENGNTNNPRWTRGNSILADSGSEQLEFIALSQRTNDPKYQQRAEKVIKQFRKI 295
PYNRINL GNT+NP+WTRGNSILADSGSEQLEFIALSQRT DPKYQ++AEKVIK+ RK
Sbjct: 238 PYNRINLAYGNTDNPKWTRGNSILADSGSEQLEFIALSQRTKDPKYQEKAEKVIKELRKR 297
Query: 296 FPEDGLLPIYIDPKTGTKPSGAITFGAMGDSFYEYLLKAWVQGNK 340
FPEDGLLPIYI+P TG K +GAITFGAMGDSFYEYLLKAW+QGNK
Sbjct: 298 FPEDGLLPIYINPLTGDKSAGAITFGAMGDSFYEYLLKAWIQGNK 342
>Medtr4g008140.1 | mannosyl-oligosaccharide 1,2-alpha-mannosidase |
HC | chr4:1398909-1405875 | 20130731
Length = 577
Score = 439 bits (1130), Expect = e-123, Method: Compositional matrix adjust.
Identities = 216/326 (66%), Positives = 258/326 (79%), Gaps = 3/326 (0%)
Query: 15 WRYVQPRYYIKRPKRLALIIIVCIALTWLVYDRQLLNKEHQEDILRLQEEVTGLQNTLEE 74
WRY P YY+KRPKRLA++ IV ++++++V+DRQ L +E+Q ++ LQ+E+T L N LEE
Sbjct: 15 WRYCNPAYYLKRPKRLAMLFIVFVSVSFVVWDRQTLVREYQVEVAELQKEITDLHNLLEE 74
Query: 75 IKGQMKGSEAESLQNDEKMNDLTNVVSVEDDPISIHRREKVKDAMLHAWTAYEKYAWGMD 134
++ G L K N T V DDPI I RREKVKDAMLHAW++YEKYAWG D
Sbjct: 75 -SNKVHGGTVRKLDLKGKTNKSTK--EVLDDPIDIQRREKVKDAMLHAWSSYEKYAWGQD 131
Query: 135 ELKPQSMNGVDSFGGLGATLVDSLDTLFIMGLDSQFRRASEWIAESLHFNKNIEVSVFET 194
EL+PQS +GV+SFGGLGATL+DSLDTL+IMGLD QF++A +W+A SL FNK+ E SVFET
Sbjct: 132 ELQPQSKSGVNSFGGLGATLIDSLDTLYIMGLDEQFQKARDWVANSLDFNKDYEASVFET 191
Query: 195 TIRVLGGLLSAYDLSGDKVFLEKARDIADKLLPAWDTPSGIPYNRINLENGNTNNPRWTR 254
TIRV+GGLLS YDLSGDK+FL+KARDIAD+LLPAW+TP+GIPYN INL G +NP WT
Sbjct: 192 TIRVVGGLLSTYDLSGDKIFLDKARDIADRLLPAWNTPTGIPYNIINLSQGRAHNPGWTG 251
Query: 255 GNSILADSGSEQLEFIALSQRTNDPKYQQRAEKVIKQFRKIFPEDGLLPIYIDPKTGTKP 314
G SILADSG+EQLEFIALSQRT DPKYQQ+ E VI K FP+DGLLPIYI+P +GT
Sbjct: 252 GESILADSGTEQLEFIALSQRTGDPKYQQKVENVITVLNKTFPDDGLLPIYINPHSGTTG 311
Query: 315 SGAITFGAMGDSFYEYLLKAWVQGNK 340
ITFGAMGDSFYEYLLK WVQGNK
Sbjct: 312 YSPITFGAMGDSFYEYLLKVWVQGNK 337
>Medtr2g019520.1 | mannosyl-oligosaccharide 1,2-alpha-mannosidase |
HC | chr2:6378959-6384832 | 20130731
Length = 631
Score = 196 bits (497), Expect = 3e-50, Method: Compositional matrix adjust.
Identities = 113/268 (42%), Positives = 164/268 (61%), Gaps = 19/268 (7%)
Query: 87 LQNDEKMNDLTNV-VSVEDDPISIHRREKVKDAMLHAWTAYEKYAWGMDELKPQSMNGVD 145
L D K+++ +V +++D + I R++KVK+A HAW+ Y+KYA G DEL P S +G D
Sbjct: 111 LPPDTKVSNNNHVPATLDDKSLWITRQQKVKEAFTHAWSGYKKYAMGYDELMPVSQHGTD 170
Query: 146 SFGGLGATLVDSLDTLFIMGLDSQFRRASEWIAESL--HFNKNIEVSVFETTIRVLGGLL 203
GGLGAT+VD+LDT IMG+D A W+ E L +K +V++FETTIRVLGGLL
Sbjct: 171 GLGGLGATVVDALDTAMIMGIDEVVAEAGLWVEEHLSERISKKGQVNLFETTIRVLGGLL 230
Query: 204 SAYDLSGDK-------------VFLEKARDIADKLLPAW-DTPSGIPYNRINLENGNTNN 249
SAY LSG + V+LE A+++ D+LL A+ +P+ IP++ + L + + +N
Sbjct: 231 SAYHLSGGEQGTNVTHAGPKPSVYLETAKNLGDRLLSAFTSSPTPIPFSDVVLHDSSAHN 290
Query: 250 PRWTRGNSILADSGSEQLEFIALSQRTNDPKYQQRAEKVIKQFRKIFPEDGLLPIYIDPK 309
G S ++ + QLEF LS + D KY A KV++ + + +GL+PIYI P
Sbjct: 291 A--PGGLSSTSEVSTLQLEFNYLSAVSGDQKYGLEAMKVMEHIKTLPKVEGLVPIYISPD 348
Query: 310 TGTKPSGAITFGAMGDSFYEYLLKAWVQ 337
+G I G+ GDS+YEYLLK W+Q
Sbjct: 349 SGQFSGENIRLGSRGDSYYEYLLKVWLQ 376
>Medtr1g076280.1 | glycosyl hydrolase family 47 protein | HC |
chr1:33971398-33960805 | 20130731
Length = 624
Score = 152 bits (383), Expect = 6e-37, Method: Compositional matrix adjust.
Identities = 94/238 (39%), Positives = 134/238 (56%), Gaps = 17/238 (7%)
Query: 112 REKVKDAMLHAWTAYEKYAWGMDELKPQSMNGVDSFGGLGATLVDSLDTLFIMGLDSQFR 171
R +V + HA+ Y A+ +DELKP S G D+ GG TL+DSLDTL ++G +F
Sbjct: 48 RNEVGEMFYHAFNGYMDNAFPLDELKPLSCKGEDTLGGYALTLIDSLDTLALLGDRERFA 107
Query: 172 RASEWIAESLHFNK-NIEVSVFETTIRVLGGLLSAYDLSGDKV-----------FLEKAR 219
+ EWI ++L F+ N VSVFETTIRVLGGL+SA+ ++ D L A
Sbjct: 108 ASVEWIGKNLRFDMINKTVSVFETTIRVLGGLISAHQIASDYATGMRVPSYDNQLLNLAE 167
Query: 220 DIADKLLPAWDTPSGIPYNRINLENG-NTNNPRWTRGNSILADSGSEQLEFIALSQRTND 278
D+A +LLPA+DTP+GIP+ +NL +G + + + T A G+ LEF LS+ TND
Sbjct: 168 DLARRLLPAFDTPTGIPFGSVNLLHGVDKHESKITS----TAGGGTLTLEFGVLSRLTND 223
Query: 279 PKYQQRAEKVIKQFRKIFPEDGLLPIYIDPKTGTKPSGAITFGAMGDSFYEYLLKAWV 336
P ++Q + + + L+ +I+ TG G DSFYEYLLKA++
Sbjct: 224 PIFEQVTKNAVLAIWARRSKLNLVGAHINVFTGEWTQKDAGIGTSIDSFYEYLLKAYL 281
>Medtr1g076280.2 | glycosyl hydrolase family 47 protein | HC |
chr1:33971330-33960839 | 20130731
Length = 552
Score = 151 bits (382), Expect = 8e-37, Method: Compositional matrix adjust.
Identities = 93/237 (39%), Positives = 131/237 (55%), Gaps = 15/237 (6%)
Query: 112 REKVKDAMLHAWTAYEKYAWGMDELKPQSMNGVDSFGGLGATLVDSLDTLFIMGLDSQFR 171
R +V + HA+ Y A+ +DELKP S G D+ GG TL+DSLDTL ++G +F
Sbjct: 48 RNEVGEMFYHAFNGYMDNAFPLDELKPLSCKGEDTLGGYALTLIDSLDTLALLGDRERFA 107
Query: 172 RASEWIAESLHFNK-NIEVSVFETTIRVLGGLLSAYDLSGDKV-----------FLEKAR 219
+ EWI ++L F+ N VSVFETTIRVLGGL+SA+ ++ D L A
Sbjct: 108 ASVEWIGKNLRFDMINKTVSVFETTIRVLGGLISAHQIASDYATGMRVPSYDNQLLNLAE 167
Query: 220 DIADKLLPAWDTPSGIPYNRINLENGNTNNPRWTRGNSILADSGSEQLEFIALSQRTNDP 279
D+A +LLPA+DTP+GIP+ +NL +G + A G+ LEF LS+ TNDP
Sbjct: 168 DLARRLLPAFDTPTGIPFGSVNLLHGVDKHESKITST---AGGGTLTLEFGVLSRLTNDP 224
Query: 280 KYQQRAEKVIKQFRKIFPEDGLLPIYIDPKTGTKPSGAITFGAMGDSFYEYLLKAWV 336
++Q + + + L+ +I+ TG G DSFYEYLLKA++
Sbjct: 225 IFEQVTKNAVLAIWARRSKLNLVGAHINVFTGEWTQKDAGIGTSIDSFYEYLLKAYL 281
>Medtr4g106370.1 | glycosyl hydrolase family 47 protein | HC |
chr4:44116468-44111121 | 20130731
Length = 570
Score = 124 bits (312), Expect = 1e-28, Method: Compositional matrix adjust.
Identities = 87/257 (33%), Positives = 129/257 (50%), Gaps = 35/257 (13%)
Query: 113 EKVKDAMLHAWTAYEKYAWGMDELKPQSMNGVDSFGGLGATLVDSLDTLF---------- 162
EKV++ HA+ Y +A+ DELKP + DS LG ++ L +
Sbjct: 39 EKVRNMFHHAYDNYMTHAFPHDELKPLTKTFTDSLSELGNLKLERLPQDYNGSALSLIES 98
Query: 163 -----IMGLDSQFRRASEWIAESLHFNKNIEVSVFETTIRVLGGLLSAYDLSGDKV---- 213
IMG +++F + W++E+L F+ + +++FE IRVLGGL+SA+ L+ D
Sbjct: 99 LSSLVIMGNNTEFEKGVRWLSENLKFDVDARINLFECNIRVLGGLVSAHLLASDSSKKLL 158
Query: 214 -------FLEKARDIADKLLPAWDTPSGIPYNRINLENGNTNNPRWTRGNSILADSGSEQ 266
L A D+ + LPA++TP+G+PY INL+ G N S GS
Sbjct: 159 QGSYKNELLGLAEDLGKRFLPAFNTPTGLPYAWINLKYGVMENETTETSTS---GCGSLI 215
Query: 267 LEFIALSQRTNDPKYQQRAEKVIKQFRKIFPEDGLLPIY---IDPKTGTKPSGAITFGAM 323
LE ALS+ T DP Y+ A ++ RK++ LL ++ +D TG + GA
Sbjct: 216 LEMGALSKLTGDPIYESVA---LRALRKLWSMQSLLKLFGTTLDVSTGQWIEHSSGIGAG 272
Query: 324 GDSFYEYLLKAWVQGNK 340
DSFYEYLLKA + K
Sbjct: 273 VDSFYEYLLKAHILFGK 289