Miyakogusa Predicted Gene

Lj4g3v1957770.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v1957770.1 tr|G7JGM0|G7JGM0_MEDTR Calcium binding
atopy-related autoantigen OS=Medicago truncatula
GN=MTR_4g116,78.66,0,EF-hand,NULL; EF_HAND_2,EF-HAND 2;
EF_hand_6,NULL; EF_hand_5,NULL; EF_hand_3,EF-hand-like domain;
CA,CUFF.49981.1
         (464 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g116190.1 | calcium-binding EF hand protein | HC | chr4:48...   707   0.0  
Medtr1g012850.1 | calcium-binding EF hand protein | HC | chr1:26...   522   e-148
Medtr3g106550.1 | calcium-binding EF hand protein | HC | chr3:49...   479   e-135
Medtr3g106550.2 | calcium-binding EF hand protein | HC | chr3:49...   409   e-114
Medtr4g107490.1 | calmodulin-domain kinase CDPK protein | HC | c...    50   5e-06

>Medtr4g116190.1 | calcium-binding EF hand protein | HC |
           chr4:48065719-48063074 | 20130731
          Length = 468

 Score =  707 bits (1824), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 348/464 (75%), Positives = 391/464 (84%), Gaps = 11/464 (2%)

Query: 1   MFSYGRSIQLCLRHNIRPLCXXXXXXXXXXXXXXXXFGGFGHNGDN---NLEWFLRSIXX 57
           +FS+GR  Q  LR+NIRPLC                F G G+ GD    + E+FLR+I  
Sbjct: 2   LFSWGRRFQQTLRYNIRPLCSQSQRSTSSSSSPLSSFDGSGNGGDERVRDFEYFLRTITS 61

Query: 58  XXXXXXXXLGYWYWSSLSSPA-------FADYNATEEQLQQNSQNRSKFLFNDGYRRRVF 110
                   LG+WYWSSLSSP+       F+DY ATE+Q QQN QN+SKFLFND YRRRVF
Sbjct: 62  GAVVVGTTLGFWYWSSLSSPSANNSLHSFSDY-ATEDQFQQNYQNKSKFLFNDNYRRRVF 120

Query: 111 FNYEKRIRLQSPPEKVFEYFATVRTPSGEVFMTPADLMRAIVPVFPPSESNRVREGFLRG 170
           FNYEKR+RLQSPPEKVFEYFA+ R P GEVFMTPADLMRAIVPVFPPSES RVREG LRG
Sbjct: 121 FNYEKRLRLQSPPEKVFEYFASNRIPGGEVFMTPADLMRAIVPVFPPSESRRVREGSLRG 180

Query: 171 EQVPGQLQCEPSDFFMLFDTNSDGLISFPEYIFFVTLLSIPESSFSVAFKMFDIDNNGEI 230
           EQ PG L+C+PS+FFMLFDT++DGLISF EYIFFVTLLSIPESSFSVAFKMFDIDNNGEI
Sbjct: 181 EQFPGSLECDPSEFFMLFDTDNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEI 240

Query: 231 DKQEFKKVMALMRSQNRQGANHRDGRRLGVKSSIENGGLLEYFFGKDGNACLQHGRFVEF 290
           DK+EFKKVM+LMRSQNRQGANHRDGRRLGVK+ IENGGLLEYFFGKDG  CLQH +FV+F
Sbjct: 241 DKEEFKKVMSLMRSQNRQGANHRDGRRLGVKTPIENGGLLEYFFGKDGKDCLQHDKFVQF 300

Query: 291 LRHLHDEILRLEFSHYDYNKKGTISAKDFALSLVASADINHINKLLDRVEELSSDQHLRD 350
           LR LHDEILRLEFSHYD+NK+GTISAKDFALSLVASADINHINKLLDRV+EL +D H+RD
Sbjct: 301 LRQLHDEILRLEFSHYDHNKRGTISAKDFALSLVASADINHINKLLDRVDELDNDPHIRD 360

Query: 351 IRITYEEFQAFAELRKKLQSFSLALFSYGKINGVLTKNDFQRAASQVCGICVTDKVVDII 410
            RIT+EEF+AFAELR+KL+SFSLA+FSYGK+NGVL K DFQRAASQVCG+ VT+ V+DII
Sbjct: 361 KRITFEEFKAFAELRRKLESFSLAIFSYGKVNGVLAKTDFQRAASQVCGVHVTENVLDII 420

Query: 411 FHVFDANRDGNLSASEFVRVIQRRENKSSGVGFGSLISCCMNCV 454
           FHVFDANRDG+LSASEFVRVIQRRE+ SS  GFG+LI+C +NCV
Sbjct: 421 FHVFDANRDGSLSASEFVRVIQRREDISSRFGFGNLITCWLNCV 464


>Medtr1g012850.1 | calcium-binding EF hand protein | HC |
           chr1:2683696-2678294 | 20130731
          Length = 464

 Score =  522 bits (1345), Expect = e-148,   Method: Compositional matrix adjust.
 Identities = 259/395 (65%), Positives = 315/395 (79%), Gaps = 14/395 (3%)

Query: 72  SSLSSPAFADYNATEEQLQQNSQNRSKFLFNDGYRRRVFFNYEKRIRLQSPPEKVFEYFA 131
           S+ +SP+F     T+  L + S     FLF    +R+VFFNYEKRIRL+SPPEKVFEYFA
Sbjct: 76  STPNSPSFL----TKFSLPETS---GAFLFRG--KRKVFFNYEKRIRLRSPPEKVFEYFA 126

Query: 132 TVRTPSGEVFMTPADLMRAIVPVFPPSESNRVREGFLRGEQVPGQLQCEPSDFFMLFDTN 191
           + R+P GEV M PADLMRA+VPVFPPSES+ VR+G+LRGE+ PG L+C PS+FFMLFD N
Sbjct: 127 SHRSPEGEVLMIPADLMRAVVPVFPPSESHLVRDGYLRGERSPGHLRCSPSEFFMLFDVN 186

Query: 192 SDGLISFPEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDKQEFKKVMALMRSQNRQGAN 251
           +DGLISF EYIFFVTLLSIPESSFSVAFKMFD+DNNGEI K+EF+KVMALMRS +RQG  
Sbjct: 187 NDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVDNNGEITKEEFQKVMALMRSHHRQGVQ 246

Query: 252 HRDGRRLGVK--SSIENGGLLEYFFGKDGNACLQHGRFVEFLRHLHDEILRLEFSHYDYN 309
           HRDG R G+K   S+ENGGLLEYFFGKDG  CLQ  +FV FL+ LHDEILRLEF HYDY 
Sbjct: 247 HRDGLRTGLKVNDSVENGGLLEYFFGKDGKGCLQLDKFVIFLQDLHDEILRLEFDHYDYK 306

Query: 310 KKGTISAKDFALSLVASADINHINKLLDRVEELSSDQHLRDIRITYEEFQAFAELRKKLQ 369
            +  ISAKDFALS+VASAD++H+ KLL+RV+EL+++   +D+RI +E+F+ FAELRKKL 
Sbjct: 307 SRKAISAKDFALSMVASADMSHLGKLLERVDELNNEPCFKDVRIRFEDFKNFAELRKKLL 366

Query: 370 SFSLALFSYGKINGVLTKNDFQRAASQVCGICVTDKVVDIIFHVFDANRDGNLSASEFVR 429
             SLALFS+GK+NG+LT++DFQRAAS VCGI ++D VV+I+FH+FD NRDGNLS  EFVR
Sbjct: 367 PLSLALFSFGKVNGLLTRDDFQRAASHVCGISLSDNVVEIVFHLFDTNRDGNLSTDEFVR 426

Query: 430 VIQRRE---NKSSGVGFGSLISCCMNCVSNLSSSK 461
           V+ +RE         G   L+SCC NC  N+SSS+
Sbjct: 427 VLHKRERDIGHPVETGMLGLLSCCWNCRENISSSR 461


>Medtr3g106550.1 | calcium-binding EF hand protein | HC |
           chr3:49246553-49249323 | 20130731
          Length = 455

 Score =  479 bits (1234), Expect = e-135,   Method: Compositional matrix adjust.
 Identities = 237/388 (61%), Positives = 296/388 (76%), Gaps = 7/388 (1%)

Query: 78  AFADYNATEEQLQQNSQNRSKFLFNDGYRRRVFFNYEKRIRLQSPPEKVFEYFATVRTPS 137
           AF +    +  L + S N    LF D YR +VFFNYEKRIRL SPPEKVFEYFA+ RTP 
Sbjct: 70  AFLNNILRKSSLPEGSNN--NLLFGDSYRSKVFFNYEKRIRLHSPPEKVFEYFASYRTPE 127

Query: 138 GEVFMTPADLMRAIVPVFPPSESNRVREGFLRGEQVPGQLQCEPSDFFMLFDTNSDGLIS 197
           GEV M PADLMRA+VPVFPPSESN VR+G L GE+ PG L C PS+FF+LFD N DGLIS
Sbjct: 128 GEVLMKPADLMRAVVPVFPPSESNLVRDGSLEGERSPGHLFCPPSEFFLLFDVNGDGLIS 187

Query: 198 FPEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDKQEFKKVMALMRSQNRQGANHRDGRR 257
           F EY+F VTLLSIPES+FS  FKMFD+DN+GEIDK+EFK+V+A MRS ++ G  HR   +
Sbjct: 188 FREYLFLVTLLSIPESNFSAVFKMFDMDNDGEIDKEEFKRVVASMRSHSKHGI-HRMYEQ 246

Query: 258 LGVKSSIENGGLLEYFFGKDGNACLQHGRFVEFLRHLHDEILRLEFSHYDYNKKGTISAK 317
               +S+ENG ++EY FGKDG   L+H +FV+F+R LHDEILRLEF HYD+  + TI AK
Sbjct: 247 -QTDASVENGRMVEYLFGKDGKGRLKHDKFVKFIRDLHDEILRLEFVHYDFKFQKTILAK 305

Query: 318 DFALSLVASADINHINKLLDRVEELSSDQHLRDIRITYEEFQAFAELRKKLQSFSLALFS 377
           DFA S+VASAD+NH++KLL+RV+EL++D     +RIT+EEF+ FAELRKKL   SLALFS
Sbjct: 306 DFAHSIVASADVNHLSKLLERVDELNNDPQFNSVRITFEEFKNFAELRKKLVPLSLALFS 365

Query: 378 YGKINGVLTKNDFQRAASQVCGICVTDKVVDIIFHVFDANRDGNLSASEFVRVIQRRE-- 435
           + K+NG+LT++DFQRAAS VCG+ ++D VV+I+FH+FD + DGNLS++EFVRV+QRRE  
Sbjct: 366 FAKVNGLLTRDDFQRAASSVCGLSLSDNVVEIVFHLFDTSGDGNLSSNEFVRVLQRRERD 425

Query: 436 -NKSSGVGFGSLISCCMNCVSNLSSSKL 462
             +S   G    +SCC NC  N   S+L
Sbjct: 426 VGRSVETGIMGFLSCCWNCTDNSPGSQL 453


>Medtr3g106550.2 | calcium-binding EF hand protein | HC |
           chr3:49246553-49249323 | 20130731
          Length = 402

 Score =  409 bits (1052), Expect = e-114,   Method: Compositional matrix adjust.
 Identities = 203/320 (63%), Positives = 249/320 (77%), Gaps = 4/320 (1%)

Query: 78  AFADYNATEEQLQQNSQNRSKFLFNDGYRRRVFFNYEKRIRLQSPPEKVFEYFATVRTPS 137
           AF +    +  L + S N    LF D YR +VFFNYEKRIRL SPPEKVFEYFA+ RTP 
Sbjct: 70  AFLNNILRKSSLPEGSNN--NLLFGDSYRSKVFFNYEKRIRLHSPPEKVFEYFASYRTPE 127

Query: 138 GEVFMTPADLMRAIVPVFPPSESNRVREGFLRGEQVPGQLQCEPSDFFMLFDTNSDGLIS 197
           GEV M PADLMRA+VPVFPPSESN VR+G L GE+ PG L C PS+FF+LFD N DGLIS
Sbjct: 128 GEVLMKPADLMRAVVPVFPPSESNLVRDGSLEGERSPGHLFCPPSEFFLLFDVNGDGLIS 187

Query: 198 FPEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDKQEFKKVMALMRSQNRQGANHRDGRR 257
           F EY+F VTLLSIPES+FS  FKMFD+DN+GEIDK+EFK+V+A MRS ++ G  HR   +
Sbjct: 188 FREYLFLVTLLSIPESNFSAVFKMFDMDNDGEIDKEEFKRVVASMRSHSKHGI-HRMYEQ 246

Query: 258 LGVKSSIENGGLLEYFFGKDGNACLQHGRFVEFLRHLHDEILRLEFSHYDYNKKGTISAK 317
               +S+ENG ++EY FGKDG   L+H +FV+F+R LHDEILRLEF HYD+  + TI AK
Sbjct: 247 -QTDASVENGRMVEYLFGKDGKGRLKHDKFVKFIRDLHDEILRLEFVHYDFKFQKTILAK 305

Query: 318 DFALSLVASADINHINKLLDRVEELSSDQHLRDIRITYEEFQAFAELRKKLQSFSLALFS 377
           DFA S+VASAD+NH++KLL+RV+EL++D     +RIT+EEF+ FAELRKKL   SLALFS
Sbjct: 306 DFAHSIVASADVNHLSKLLERVDELNNDPQFNSVRITFEEFKNFAELRKKLVPLSLALFS 365

Query: 378 YGKINGVLTKNDFQRAASQV 397
           + K+NG+LT++DFQRAAS V
Sbjct: 366 FAKVNGLLTRDDFQRAASSV 385


>Medtr4g107490.1 | calmodulin-domain kinase CDPK protein | HC |
           chr4:44509014-44514153 | 20130731
          Length = 555

 Score = 50.1 bits (118), Expect = 5e-06,   Method: Compositional matrix adjust.
 Identities = 52/196 (26%), Positives = 81/196 (41%), Gaps = 35/196 (17%)

Query: 219 FKMFDIDNNGEIDKQEFKKVMALMRSQNRQGANHRDGRRLGVKSSIENGGLLEYFFGKDG 278
           F + D D +G I  +E K  +                R++G + +     LL      DG
Sbjct: 383 FTLMDTDKDGRITYEELKAGL----------------RKVGSQLAEPEIKLLMDVADVDG 426

Query: 279 NACLQHGRFVEFLRHLH----DEILRLEFSHYDYNKKGTISAKDFALSLV---ASADINH 331
           N  L +G FV    HL     DE  R  F  +D ++ G I   +   +LV      D N 
Sbjct: 427 NGVLDYGEFVAVTIHLQRMENDEHFRKAFKFFDKDESGYIELSELEAALVDDLGETDTNV 486

Query: 332 INKLLDRVEELSSDQHLRDIRITYEEFQAF----AELRKKLQSFSLALFSYGKINGVLTK 387
           +N+++    E+ +D   +D RI+YEEF A      + RK  + +S   F    IN  L K
Sbjct: 487 LNEIM---REVDTD---KDGRISYEEFVAMMKTGTDWRKASRQYSRERFKSLSIN--LMK 538

Query: 388 NDFQRAASQVCGICVT 403
           +   +   ++ G  V 
Sbjct: 539 DGSLQLHDEISGQAVV 554