Miyakogusa Predicted Gene
- Lj4g3v1635340.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj4g3v1635340.1 Non Characterized Hit- tr|I3SFA8|I3SFA8_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,99.33,0,Ldh_1_C,Lactate/malate dehydrogenase, C-terminal;
Ldh_1_N,Lactate/malate dehydrogenase, N-terminal; ,gene.g55383.t1.1
(298 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr5g014710.1 | glyoxysomal malate dehydrogenase | HC | chr5:4... 388 e-108
Medtr4g092690.1 | glyoxysomal malate dehydrogenase | HC | chr4:3... 374 e-104
Medtr8g463760.1 | malate dehydrogenase | HC | chr8:22494348-2249... 367 e-102
Medtr8g463760.2 | malate dehydrogenase | HC | chr8:22494347-2249... 230 1e-60
Medtr2g045010.1 | malate dehydrogenase | HC | chr2:19586812-1958... 76 4e-14
Medtr2g021700.1 | malate dehydrogenase | HC | chr2:7390300-73922... 76 4e-14
Medtr4g033285.1 | malate dehydrogenase | LC | chr4:11658666-1165... 68 1e-11
Medtr1g043040.1 | cytoplasmic-like malate dehydrogenase | HC | c... 57 2e-08
Medtr8g005980.1 | cytoplasmic-like malate dehydrogenase | HC | c... 56 4e-08
Medtr1g076130.1 | cytoplasmic-like malate dehydrogenase | HC | c... 54 2e-07
Medtr4g101130.1 | L-lactate dehydrogenase A-like protein | HC | ... 48 9e-06
>Medtr5g014710.1 | glyoxysomal malate dehydrogenase | HC |
chr5:4992016-4987668 | 20130731
Length = 358
Score = 388 bits (997), Expect = e-108, Method: Compositional matrix adjust.
Identities = 190/293 (64%), Positives = 234/293 (79%), Gaps = 2/293 (0%)
Query: 1 MSPLVSDLHLYDIANVKGVAADISHCNTPSQVRDFTGASELGDCLKGVNVVVIPAGVPRK 60
++PLVS LHLYD+ N GV +DISH +T + VR F G ++L D L G+++V+IPAGVPRK
Sbjct: 68 INPLVSVLHLYDVVNTPGVTSDISHMDTSAVVRGFLGQNQLEDALTGMDLVIIPAGVPRK 127
Query: 61 PGMTRDDLFNINAGIVRDLVSAVADNCPGAFIHIISNPVNSTVPIAAEILKQKGVYDPKK 120
PGMTRDDLFNINAGIV+ L A+A CP A +++ISNPVNSTVPIAAE+ K+ G YDPK+
Sbjct: 128 PGMTRDDLFNINAGIVKTLCEAIAKQCPKAIVNLISNPVNSTVPIAAEVFKRAGTYDPKR 187
Query: 121 LFGVTTLDVVRANTFVAQRKNLKLIDVDVPVVGGHAGITILPLLSKTRPSASFTDEEIEE 180
L GVT LDVVRANTFVA+ L DVDVPVVGGHAGITILPLLS+ +P +SFT +EIE
Sbjct: 188 LLGVTMLDVVRANTFVAEVMGLDPRDVDVPVVGGHAGITILPLLSQVKPPSSFTPKEIEY 247
Query: 181 LTVRIQNAGTEVVEAKAGAGSATLSMAYAAARFVESSLRALDGDGDVYECAYVQSDLTEL 240
LT RIQN GTEVVEAKAGAGSATLSMAYAA +F ++ LRAL G+ D+ +CAYV S +TEL
Sbjct: 248 LTDRIQNGGTEVVEAKAGAGSATLSMAYAAVKFADACLRALKGEADIVQCAYVDSQVTEL 307
Query: 241 PFFASRVKLGKKGVEALIPSDLQGLTEYEQKALEALKPELKSSIEKGVAFAQK 293
PFFAS+V+LG+ GVE +P L L++YE+ +LE K EL +S+EKGV+F +K
Sbjct: 308 PFFASKVRLGRNGVEEFLP--LGPLSDYERASLEKAKKELATSVEKGVSFIRK 358
>Medtr4g092690.1 | glyoxysomal malate dehydrogenase | HC |
chr4:36762718-36765660 | 20130731
Length = 356
Score = 374 bits (961), Expect = e-104, Method: Compositional matrix adjust.
Identities = 188/293 (64%), Positives = 222/293 (75%), Gaps = 2/293 (0%)
Query: 1 MSPLVSDLHLYDIANVKGVAADISHCNTPSQVRDFTGASELGDCLKGVNVVVIPAGVPRK 60
M+PLVS LHLYD+ N GV AD+SH +T + VR F G +L + L G+++VVIPAGVPRK
Sbjct: 66 MNPLVSVLHLYDVVNTPGVTADVSHMDTGAVVRGFLGQPQLENALTGMDLVVIPAGVPRK 125
Query: 61 PGMTRDDLFNINAGIVRDLVSAVADNCPGAFIHIISNPVNSTVPIAAEILKQKGVYDPKK 120
PGMTRDDLF INAGIVR L +A CP A +++ISNPVNSTVPIAAE+ K+ G YDPK+
Sbjct: 126 PGMTRDDLFKINAGIVRTLCEGIAKCCPNAIVNLISNPVNSTVPIAAEVFKKAGTYDPKR 185
Query: 121 LFGVTTLDVVRANTFVAQRKNLKLIDVDVPVVGGHAGITILPLLSKTRPSASFTDEEIEE 180
L GVT LDVVRANTFVA+ + DVDVPVVGGHAG+TILPLLS+ +P +SFT EE E
Sbjct: 186 LLGVTALDVVRANTFVAEVLGVDPRDVDVPVVGGHAGVTILPLLSQVKPPSSFTAEETEY 245
Query: 181 LTVRIQNAGTEVVEAKAGAGSATLSMAYAAARFVESSLRALDGDGDVYECAYVQSDLTEL 240
LT RIQN GTEVVEAKAGAGSATLSMAYAAA+F S L L G+ V ECAYV S +TEL
Sbjct: 246 LTNRIQNGGTEVVEAKAGAGSATLSMAYAAAKFANSCLHGLKGEAGVIECAYVDSQVTEL 305
Query: 241 PFFASRVKLGKKGVEALIPSDLQGLTEYEQKALEALKPELKSSIEKGVAFAQK 293
PFFA++V+LG+ G E + P L L EYE+ LE K EL SI+KGV F +K
Sbjct: 306 PFFATKVRLGRAGAEEIFP--LGPLNEYERIGLEKAKRELAGSIQKGVEFIKK 356
>Medtr8g463760.1 | malate dehydrogenase | HC |
chr8:22494348-22490385 | 20130731
Length = 343
Score = 367 bits (941), Expect = e-102, Method: Compositional matrix adjust.
Identities = 180/294 (61%), Positives = 228/294 (77%), Gaps = 3/294 (1%)
Query: 1 MSPLVSDLHLYDIANVKGVAADISHCNTPSQVRDFTGASELGDCLKGVNVVVIPAGVPRK 60
++PLVS L LYDIA GVAAD+SH N+ SQV + G ELG L+G +VV+IPAGVPRK
Sbjct: 52 LNPLVSTLSLYDIAGTPGVAADVSHINSRSQVTGYAGEDELGKALEGADVVIIPAGVPRK 111
Query: 61 PGMTRDDLFNINAGIVRDLVSAVADNCPGAFIHIISNPVNSTVPIAAEILKQKGVYDPKK 120
PGMTRDDLFNINAGIV+ L +A++ CP A +++ISNPVNSTVPIAAE+ K+ G YD K+
Sbjct: 112 PGMTRDDLFNINAGIVKSLATAISKYCPHALVNMISNPVNSTVPIAAEVFKKAGTYDEKR 171
Query: 121 LFGVTTLDVVRANTFVAQRKNLKLIDVDVPVVGGHAGITILPLLSKTRPSASFTDEEIEE 180
LFGVTTLDVVRA TF A + N+ + +V+VPV+GGHAG+TILPL S+ P A+ D+ I+
Sbjct: 172 LFGVTTLDVVRAKTFYAGKANVPVAEVNVPVIGGHAGVTILPLFSQASPQANLDDDVIKA 231
Query: 181 LTVRIQNAGTEVVEAKAGAGSATLSMAYAAARFVESSLRALDGDGDVYECAYVQSDL-TE 239
LT R Q+ GTEVV AKAG GSATLSMAYA A F ++ L+ L+G DV EC+YVQS+L E
Sbjct: 232 LTARTQDGGTEVVTAKAGKGSATLSMAYAGAIFADACLKGLNGVPDVVECSYVQSNLIAE 291
Query: 240 LPFFASRVKLGKKGVEALIPSDLQGLTEYEQKALEALKPELKSSIEKGVAFAQK 293
LPFFAS+V++GK GVE ++ L L+++E++ LE LK ELKSSIEKG+ FA +
Sbjct: 292 LPFFASKVRIGKNGVEEIL--GLGSLSDFEKQGLENLKSELKSSIEKGIKFASQ 343
>Medtr8g463760.2 | malate dehydrogenase | HC |
chr8:22494347-22490385 | 20130731
Length = 251
Score = 230 bits (586), Expect = 1e-60, Method: Compositional matrix adjust.
Identities = 108/166 (65%), Positives = 134/166 (80%)
Query: 1 MSPLVSDLHLYDIANVKGVAADISHCNTPSQVRDFTGASELGDCLKGVNVVVIPAGVPRK 60
++PLVS L LYDIA GVAAD+SH N+ SQV + G ELG L+G +VV+IPAGVPRK
Sbjct: 52 LNPLVSTLSLYDIAGTPGVAADVSHINSRSQVTGYAGEDELGKALEGADVVIIPAGVPRK 111
Query: 61 PGMTRDDLFNINAGIVRDLVSAVADNCPGAFIHIISNPVNSTVPIAAEILKQKGVYDPKK 120
PGMTRDDLFNINAGIV+ L +A++ CP A +++ISNPVNSTVPIAAE+ K+ G YD K+
Sbjct: 112 PGMTRDDLFNINAGIVKSLATAISKYCPHALVNMISNPVNSTVPIAAEVFKKAGTYDEKR 171
Query: 121 LFGVTTLDVVRANTFVAQRKNLKLIDVDVPVVGGHAGITILPLLSK 166
LFGVTTLDVVRA TF A + N+ + +V+VPV+GGHAG+TILPL S+
Sbjct: 172 LFGVTTLDVVRAKTFYAGKANVPVAEVNVPVIGGHAGVTILPLFSQ 217
>Medtr2g045010.1 | malate dehydrogenase | HC |
chr2:19586812-19584816 | 20130731
Length = 150
Score = 75.9 bits (185), Expect = 4e-14, Method: Compositional matrix adjust.
Identities = 36/51 (70%), Positives = 43/51 (84%)
Query: 146 DVDVPVVGGHAGITILPLLSKTRPSASFTDEEIEELTVRIQNAGTEVVEAK 196
+VDVPVVGGHAG+TILPLLS+ + +SFT EE E LT RIQN GTEVVE++
Sbjct: 52 EVDVPVVGGHAGVTILPLLSQAKSPSSFTAEETEYLTNRIQNGGTEVVESQ 102
>Medtr2g021700.1 | malate dehydrogenase | HC | chr2:7390300-7392293
| 20130731
Length = 150
Score = 75.9 bits (185), Expect = 4e-14, Method: Compositional matrix adjust.
Identities = 36/51 (70%), Positives = 43/51 (84%)
Query: 146 DVDVPVVGGHAGITILPLLSKTRPSASFTDEEIEELTVRIQNAGTEVVEAK 196
+VDVPVVGGHAG+TILPLLS+ + +SFT EE E LT RIQN GTEVVE++
Sbjct: 52 EVDVPVVGGHAGVTILPLLSQAKSPSSFTAEETEYLTNRIQNGGTEVVESQ 102
>Medtr4g033285.1 | malate dehydrogenase | LC |
chr4:11658666-11657042 | 20130731
Length = 96
Score = 67.8 bits (164), Expect = 1e-11, Method: Compositional matrix adjust.
Identities = 32/45 (71%), Positives = 37/45 (82%)
Query: 146 DVDVPVVGGHAGITILPLLSKTRPSASFTDEEIEELTVRIQNAGT 190
+VDVPVVGGHAG+TILPLLS+ + +SFT EE E LT RIQN GT
Sbjct: 52 EVDVPVVGGHAGVTILPLLSQDKSPSSFTAEETEYLTNRIQNGGT 96
>Medtr1g043040.1 | cytoplasmic-like malate dehydrogenase | HC |
chr1:16122538-16117304 | 20130731
Length = 332
Score = 57.0 bits (136), Expect = 2e-08, Method: Compositional matrix adjust.
Identities = 49/183 (26%), Positives = 91/183 (49%), Gaps = 17/183 (9%)
Query: 8 LHLYDIA----NVKGVAADISHCNTPSQVRDFTGASELGDCLKGVNVVVIPAGVPRKPGM 63
LH+ DIA ++ GV ++ P ++ +++ + GVN+ V+ G PRK GM
Sbjct: 39 LHMLDIAPAAESLNGVKMELVDAAFP-LLKGVVATTDVVEACTGVNIAVMVGGFPRKEGM 97
Query: 64 TRDDLFNINAGIVRDLVSAV----ADNCPGAFIHIISNPVNSTVPIAAEILKQKGVYDPK 119
R D+ + N I + SA+ A NC + +++NP N+ A ILK+ P+
Sbjct: 98 ERKDVMSKNVSIYKSQASALEKHAAANCK---VLVVANPANTN----ALILKEFAPSIPE 150
Query: 120 K-LFGVTTLDVVRANTFVAQRKNLKLIDVDVPVVGGHAGITILPLLSKTRPSASFTDEEI 178
+ + +T LD RA +++R N+++ DV ++ G+ T P ++ + ++ +
Sbjct: 151 RNISCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSTQYPDVNHATVNTPAGEKPV 210
Query: 179 EEL 181
EL
Sbjct: 211 REL 213
>Medtr8g005980.1 | cytoplasmic-like malate dehydrogenase | HC |
chr8:430275-432634 | 20130731
Length = 413
Score = 56.2 bits (134), Expect = 4e-08, Method: Compositional matrix adjust.
Identities = 52/203 (25%), Positives = 94/203 (46%), Gaps = 11/203 (5%)
Query: 8 LHLYDIA----NVKGVAADISHCNTPSQVRDFTGASELGDCLKGVNVVVIPAGVPRKPGM 63
LH+ DI +KGV ++ P +R +++ + K VN+ V+ G PRK GM
Sbjct: 121 LHMLDIEPALEALKGVKMELIDAAFP-LLRGVVATTDVVEACKDVNIAVMVGGFPRKEGM 179
Query: 64 TRDDLFNINAGIVRDLVSAVADNCPGAF-IHIISNPVNSTVPIAAEILKQKGVYDPKK-L 121
R D+ + N I + SA+ ++ + +I+NP N+ A ILK+ P+K +
Sbjct: 180 ERKDVMSKNVSIYKTQASALEEHAAAYCKVLVIANPANTN----ALILKEFAPSIPEKNI 235
Query: 122 FGVTTLDVVRANTFVAQRKNLKLIDVDVPVVGGHAGITILPLLSKTRPSASFTDEEIEEL 181
+T LD RA ++++ N+ + DV ++ G+ T P ++ + S + + EL
Sbjct: 236 TCLTRLDHNRALGQISEKLNVDVSDVKNVIIWGNHSSTQYPDVNHATVATSNGKKHVREL 295
Query: 182 TVRIQNAGTEVVEAKAGAGSATL 204
+E + G A +
Sbjct: 296 VADDNWLNSEFITTVQQRGGAII 318
>Medtr1g076130.1 | cytoplasmic-like malate dehydrogenase | HC |
chr1:33721743-33725370 | 20130731
Length = 332
Score = 53.5 bits (127), Expect = 2e-07, Method: Compositional matrix adjust.
Identities = 48/183 (26%), Positives = 89/183 (48%), Gaps = 17/183 (9%)
Query: 8 LHLYDI----ANVKGVAADISHCNTPSQVRDFTGASELGDCLKGVNVVVIPAGVPRKPGM 63
LH+ DI ++ GV ++ P ++ +++ + GVN+ V+ G PRK GM
Sbjct: 39 LHMLDIPPAAESLNGVKMELVDAAFP-LLKGVVATTDVVEGCTGVNIAVMVGGFPRKEGM 97
Query: 64 TRDDLFNINAGIVRDLVSAV----ADNCPGAFIHIISNPVNSTVPIAAEILKQKGVYDPK 119
R D+ N I + SA+ A NC + +++NP N+ A ILK+ P+
Sbjct: 98 ERKDVMTKNVSIYKSQASALEKHAAANCK---VLVVANPANTN----ALILKEFAPSIPE 150
Query: 120 K-LFGVTTLDVVRANTFVAQRKNLKLIDVDVPVVGGHAGITILPLLSKTRPSASFTDEEI 178
K + +T LD RA +++R N+++ +V ++ G+ + P ++ S ++ +
Sbjct: 151 KNISCLTRLDHNRALGQISERLNVEVSNVKNVIIWGNHSSSQYPDVNHATVKISSAEKPV 210
Query: 179 EEL 181
EL
Sbjct: 211 REL 213
>Medtr4g101130.1 | L-lactate dehydrogenase A-like protein | HC |
chr4:41764793-41767250 | 20130731
Length = 350
Score = 48.1 bits (113), Expect = 9e-06, Method: Compositional matrix adjust.
Identities = 44/186 (23%), Positives = 78/186 (41%), Gaps = 21/186 (11%)
Query: 47 GVNVVVIPAGVPRKPGMTRDDLFNINAGIVRDLVSAVADNCPGAFIHIISNPVNSTVPIA 106
G ++ ++ AG + G +R +L N + + ++ +A P + I+SNPV+ IA
Sbjct: 105 GSDLCIVTAGARQIGGESRLNLLQRNLSLFKAIIPPLARYSPETVLIIVSNPVDVLTYIA 164
Query: 107 AEILKQKGVYDPKKLFGVTTLDVVRANTFVAQRKNLKLIDVDVPVVGGH----------- 155
K G + + T LD R +A + DV +VG H
Sbjct: 165 ---WKLSGFPSNRVIGSGTNLDSSRFCFLIADHLQVNAQDVQACIVGEHGDSSVALWSSI 221
Query: 156 --AGITILPLLSKTRPSASFTDEEIEELTVRIQNAGTEVVEAKAGAGSATLSMAYAAARF 213
GI +L L K + ++ E +E + + N+ EV+ K G + ++ Y+ A
Sbjct: 222 SIGGIPVLSFLEKQQ--IAYEKETLENIHKTVINSAYEVISLK---GYTSWAIGYSVASL 276
Query: 214 VESSLR 219
S +R
Sbjct: 277 ARSIIR 282