Miyakogusa Predicted Gene

Lj4g3v1302690.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v1302690.1 tr|G7JK82|G7JK82_MEDTR Sterol-4-methyl-oxidase
OS=Medicago truncatula GN=MTR_4g102060 PE=4 SV=1,86.8,0,seg,NULL;
FA_hydroxylase,Fatty acid hydroxylase; SUBFAMILY NOT NAMED,NULL;
STEROL DESATURASE,NULL,CUFF.48787.1
         (302 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g102060.1 | sterol C4-methyl oxidase 1-2 | HC | chr4:42239...   519   e-147
Medtr7g074900.1 | C-4 methylsterol oxidase | HC | chr7:28035902-...   379   e-105
Medtr6g039970.1 | C-4 methylsterol oxidase | HC | chr6:14370848-...   190   1e-48
Medtr8g063210.1 | C-4 methylsterol oxidase | HC | chr8:26467122-...   187   1e-47
Medtr5g035190.1 | C-4 methylsterol oxidase | HC | chr5:15313529-...   186   3e-47
Medtr5g035190.2 | C-4 methylsterol oxidase | HC | chr5:15314728-...   172   3e-43
Medtr5g040320.1 | sphingoid base hydroxylase-like protein | HC |...    52   7e-07

>Medtr4g102060.1 | sterol C4-methyl oxidase 1-2 | HC |
           chr4:42239567-42243413 | 20130731
          Length = 303

 Score =  519 bits (1337), Expect = e-147,   Method: Compositional matrix adjust.
 Identities = 250/303 (82%), Positives = 271/303 (89%), Gaps = 1/303 (0%)

Query: 1   MLPYHSIHEATAALGRNLTAAETLWFNYSATKSDYFLYCHNILFLFLIFSLVPLPFVFLE 60
           M+PY S+H+A  +LGRNLT AETLWFNYSATKSDYFL+CHNILFLFLIFSLVPLP VFLE
Sbjct: 1   MIPYQSLHDAEISLGRNLTFAETLWFNYSATKSDYFLFCHNILFLFLIFSLVPLPLVFLE 60

Query: 61  IKRLSPFDDYKIQPKVRLSLRDMFVCYKSVMRMFFLVVGPLQLVSYPSIQIIGIRTGLPL 120
           IKRL PFD+YKIQPKVRLSL +M  CYK V++MFFLVVGPLQL+SYPSI++IGIRTGLPL
Sbjct: 61  IKRLYPFDEYKIQPKVRLSLHEMLNCYKDVLKMFFLVVGPLQLISYPSIKMIGIRTGLPL 120

Query: 121 PSGWEILCQLLVYFVVEDYTNYWIHRFLHNKWGYEKIHRVHHEYQAPIGYAAPYAHWAEI 180
           PSG+EIL QLLVYF+VEDYTNYWIHRFLHNKWGYEKIHRVHHEYQAPIG+AAPYAHWAEI
Sbjct: 121 PSGFEILSQLLVYFLVEDYTNYWIHRFLHNKWGYEKIHRVHHEYQAPIGFAAPYAHWAEI 180

Query: 181 LILGIPSFLGPAMVPGHIITFWLWIALRQIEAIDTHSGYDFPWNPTKYIPFXXXXXXXXX 240
           LILGIPSFLGPAMVPGH+ITFWLWIALRQ+EAIDTHSGYDFPWNPTKYIPF         
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQVEAIDTHSGYDFPWNPTKYIPFYGGAEHHDY 240

Query: 241 XXXXXXQSQSNFASVFTYCDYIYGTDKGYRYQKKILRKLKEDLTNGADQNGGSYKTQ-SY 299
                 QSQSNFASVFTYCDYIYGTDKG+RYQKKIL+KLKED TNGA QNG SY  Q +Y
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGFRYQKKILQKLKEDSTNGAAQNGRSYSEQENY 300

Query: 300 KSD 302
           KS+
Sbjct: 301 KSE 303


>Medtr7g074900.1 | C-4 methylsterol oxidase | HC |
           chr7:28035902-28030735 | 20130731
          Length = 293

 Score =  379 bits (972), Expect = e-105,   Method: Compositional matrix adjust.
 Identities = 176/302 (58%), Positives = 221/302 (73%), Gaps = 9/302 (2%)

Query: 1   MLPYHSIHEATAALGRNLTAAETLWFNYSATKSDYFLYCHNILFLFLIFSLVPLPFVFLE 60
           MLPY ++ E+  ALGRNLT AETLWFNYSA K+D+ L+CHN LFL L +S+ P+PFV +E
Sbjct: 1   MLPYQTLDESKHALGRNLTFAETLWFNYSAKKTDFVLHCHNTLFLCLFYSIAPIPFVLIE 60

Query: 61  IKRLSPFDDYKIQPKVRLSLRDMFVCYKSVMRMFFLVVGPLQLVSYPSIQIIGIRTGLPL 120
           +      + YKIQP V  +L +MF CYK VM  F + VGPLQ++SYP+I+ +GIRTGLPL
Sbjct: 61  LSGYKKLNKYKIQPSVNKTLLEMFKCYKYVMWTFIIAVGPLQIISYPTIKWLGIRTGLPL 120

Query: 121 PSGWEILCQLLVYFVVEDYTNYWIHRFLHNKWGYEKIHRVHHEYQAPIGYAAPYAHWAEI 180
           PSGWE+   L+VY ++EDY NYW HR LHNKW ++ IH+VHHEY+ PI  AAPYAHW+EI
Sbjct: 121 PSGWELFWHLIVYVIIEDYVNYWFHRMLHNKWAFDNIHKVHHEYKTPIALAAPYAHWSEI 180

Query: 181 LILGIPSFLGPAMVPGHIITFWLWIALRQIEAIDTHSGYDFPWNPTKYIPFXXXXXXXXX 240
           LILG P+FLGPA+VPGHI T+WLW  LRQ+EAI+THSGY+ PW+PTKYIPF         
Sbjct: 181 LILGFPAFLGPALVPGHITTYWLWFILRQLEAIETHSGYELPWSPTKYIPFYGGPAYHDY 240

Query: 241 XXXXXXQSQSNFASVFTYCDYIYGTDKGYRYQKKILRKLKEDLTNGADQNGGSYKTQSYK 300
                 +SQ NFASVFTYCDYIYGT KGY+Y+K++  K+    T         + +Q+YK
Sbjct: 241 HHYVGGRSQGNFASVFTYCDYIYGTIKGYQYKKRMYEKISPTYT---------FNSQNYK 291

Query: 301 SD 302
            D
Sbjct: 292 VD 293


>Medtr6g039970.1 | C-4 methylsterol oxidase | HC |
           chr6:14370848-14368250 | 20130731
          Length = 271

 Score =  190 bits (482), Expect = 1e-48,   Method: Compositional matrix adjust.
 Identities = 103/269 (38%), Positives = 146/269 (54%), Gaps = 4/269 (1%)

Query: 22  ETLWFNYSATKSDYFLYCHNILFLF-LIFSLVPLPFVFLEIKRLSPFDDYKIQPKVRLSL 80
           E+ W       SD+ L C    FL  ++F L  LPF++LE  R     +YKIQ K+  S 
Sbjct: 6   ESAWQYLIINFSDFQLACFGTFFLHEIVFFLSGLPFIWLE--RAGWLSNYKIQTKIN-SP 62

Query: 81  RDMFVCYKSVMRMFFLVVGPLQLVSYPSIQIIGIRTGLPLPSGWEILCQLLVYFVVEDYT 140
                C   ++ + F V  P  + SYP  + +G+ + LPLPS   +L Q++ YF++ED+ 
Sbjct: 63  ESKERCIIRLLVLHFGVNLPAMIFSYPVFKYMGMHSSLPLPSWRVVLTQIIFYFILEDFL 122

Query: 141 NYWIHRFLHNKWGYEKIHRVHHEYQAPIGYAAPYAHWAEILILGIPSFLGPAMVPGHIIT 200
            YW HR +H KW Y+ +HRVHHEY  P G  + Y H AEIL LG P+ LGPA+   H+IT
Sbjct: 123 FYWEHRIMHTKWLYKHVHRVHHEYATPFGLTSEYGHPAEILFLGFPTMLGPAITGPHLIT 182

Query: 201 FWLWIALRQIEAIDTHSGYDFPWNPTKYIPFXXXXXXXXXXXXXXXQSQSNFASVFTYCD 260
            WL+  LR +E ++ H GY FPW+P+ ++P                    N++S FTY D
Sbjct: 183 LWLYTVLRVLETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRVLYTKSGNYSSTFTYMD 242

Query: 261 YIYGTDKGYRYQKKILRKLKEDLTNGADQ 289
            I+GTD GYR  K +     E  +   +Q
Sbjct: 243 RIFGTDIGYRKLKALKNTGVEYSSEQMNQ 271


>Medtr8g063210.1 | C-4 methylsterol oxidase | HC |
           chr8:26467122-26463827 | 20130731
          Length = 271

 Score =  187 bits (474), Expect = 1e-47,   Method: Compositional matrix adjust.
 Identities = 101/255 (39%), Positives = 140/255 (54%), Gaps = 4/255 (1%)

Query: 22  ETLWFNYSATKSDYFLYCHNILFLF-LIFSLVPLPFVFLEIKRLSPFDDYKIQPKVRLSL 80
           E+ W       SD+ L C    FL   +F L  LPF++LE  R      YKIQ K   S 
Sbjct: 6   ESAWQYLITNFSDFQLACLGSFFLHESVFFLSGLPFIWLE--RAGWLSKYKIQAKTN-SR 62

Query: 81  RDMFVCYKSVMRMFFLVVGPLQLVSYPSIQIIGIRTGLPLPSGWEILCQLLVYFVVEDYT 140
                C   ++   F V  P+ + SYP  + +G+++ LPLPS   +L Q++ YF++ED+ 
Sbjct: 63  ESEERCIIRLLVYHFGVNLPVMIFSYPVFKYMGMQSSLPLPSWRVVLTQIIFYFILEDFV 122

Query: 141 NYWIHRFLHNKWGYEKIHRVHHEYQAPIGYAAPYAHWAEILILGIPSFLGPAMVPGHIIT 200
            YW HR LH KW Y+ +H VHHEY  P G  + YAH AEIL LG  + +GPA+   H+IT
Sbjct: 123 FYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLIT 182

Query: 201 FWLWIALRQIEAIDTHSGYDFPWNPTKYIPFXXXXXXXXXXXXXXXQSQSNFASVFTYCD 260
            WLW+ LR +E ++ H GY FPW+P+ ++P                    N++S FTY D
Sbjct: 183 LWLWMVLRVLETVEAHCGYHFPWSPSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFTYMD 242

Query: 261 YIYGTDKGYRYQKKI 275
            I+GTD GYR  K +
Sbjct: 243 RIFGTDIGYRKLKAL 257


>Medtr5g035190.1 | C-4 methylsterol oxidase | HC |
           chr5:15313529-15317196 | 20130731
          Length = 271

 Score =  186 bits (471), Expect = 3e-47,   Method: Compositional matrix adjust.
 Identities = 102/251 (40%), Positives = 140/251 (55%), Gaps = 4/251 (1%)

Query: 33  SDYFLYCHNILFLF-LIFSLVPLPFVFLEIKRLSPFDDYKIQPKVRLSLRDMFVCYKSVM 91
           SD+ L C    FL   +F L  LPFV++E  R      YKIQ K   S      C   ++
Sbjct: 17  SDFQLACLGSFFLHESVFFLSGLPFVWIE--RAGWLSKYKIQAKNN-SPEAQDKCIVRLL 73

Query: 92  RMFFLVVGPLQLVSYPSIQIIGIRTGLPLPSGWEILCQLLVYFVVEDYTNYWIHRFLHNK 151
              F V  P+ + SYP  + +G+++ LPLPS   IL Q++ YF++ED+  YW HR LH K
Sbjct: 74  LYHFGVNLPVMIFSYPVFRYMGMQSSLPLPSWNIILTQIMFYFILEDFIFYWGHRILHTK 133

Query: 152 WGYEKIHRVHHEYQAPIGYAAPYAHWAEILILGIPSFLGPAMVPGHIITFWLWIALRQIE 211
           W Y+ IH VHHEY  P G  + YAH AEIL LG  + +GPA+   H+IT WLW+ +R +E
Sbjct: 134 WLYKHIHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLITLWLWMVVRVLE 193

Query: 212 AIDTHSGYDFPWNPTKYIPFXXXXXXXXXXXXXXXQSQSNFASVFTYCDYIYGTDKGYRY 271
            ++ H GY FPW+P+ ++P                    N++S FTY D I+GTD GYR 
Sbjct: 194 TVEAHCGYHFPWSPSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDVGYRK 253

Query: 272 QKKILRKLKED 282
            K +  +  ED
Sbjct: 254 LKALKSREFED 264


>Medtr5g035190.2 | C-4 methylsterol oxidase | HC |
           chr5:15314728-15317193 | 20130731
          Length = 217

 Score =  172 bits (437), Expect = 3e-43,   Method: Compositional matrix adjust.
 Identities = 82/197 (41%), Positives = 116/197 (58%)

Query: 86  CYKSVMRMFFLVVGPLQLVSYPSIQIIGIRTGLPLPSGWEILCQLLVYFVVEDYTNYWIH 145
           C   ++   F V  P+ + SYP  + +G+++ LPLPS   IL Q++ YF++ED+  YW H
Sbjct: 14  CIVRLLLYHFGVNLPVMIFSYPVFRYMGMQSSLPLPSWNIILTQIMFYFILEDFIFYWGH 73

Query: 146 RFLHNKWGYEKIHRVHHEYQAPIGYAAPYAHWAEILILGIPSFLGPAMVPGHIITFWLWI 205
           R LH KW Y+ IH VHHEY  P G  + YAH AEIL LG  + +GPA+   H+IT WLW+
Sbjct: 74  RILHTKWLYKHIHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLITLWLWM 133

Query: 206 ALRQIEAIDTHSGYDFPWNPTKYIPFXXXXXXXXXXXXXXXQSQSNFASVFTYCDYIYGT 265
            +R +E ++ H GY FPW+P+ ++P                    N++S FTY D I+GT
Sbjct: 134 VVRVLETVEAHCGYHFPWSPSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFTYMDRIFGT 193

Query: 266 DKGYRYQKKILRKLKED 282
           D GYR  K +  +  ED
Sbjct: 194 DVGYRKLKALKSREFED 210


>Medtr5g040320.1 | sphingoid base hydroxylase-like protein | HC |
           chr5:17732499-17735533 | 20130731
          Length = 263

 Score = 52.0 bits (123), Expect = 7e-07,   Method: Compositional matrix adjust.
 Identities = 35/101 (34%), Positives = 50/101 (49%), Gaps = 8/101 (7%)

Query: 129 QLLVYFVVEDYTNYWIHRFLH-NKWGYEKIHRVHHEYQAPIGYAAPYAHWAEILIL---- 183
           Q +   +V D   Y++HR++H NK+ Y+ IH +HH    P  + A Y H  E L+L    
Sbjct: 101 QFITAMLVMDTWQYFMHRYMHQNKFLYKHIHSLHHRLIVPYSFGALYNHPIEGLLLDTIG 160

Query: 184 GIPSFLGPAMVPGHIITFWLWIALRQIEAIDTHSGYDFPWN 224
           G  SFL   M P   I F+   +   I+ +D H G   P N
Sbjct: 161 GALSFLLSGMSPRASIFFF---SFATIKTVDDHCGLWLPGN 198