Miyakogusa Predicted Gene

Lj4g3v1212310.2

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v1212310.2 Non Characterized Hit- tr|I1MV77|I1MV77_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.21664
PE,85.27,0,alpha/beta knot,NULL; SpoU_methylase,tRNA/rRNA
methyltransferase, SpoU; 23S RRNA METHYLTRANSFERASE,N,CUFF.48635.2
         (404 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g099300.1 | tRNA/rRNA methyltransferase SpoU family protei...   666   0.0  
Medtr7g012240.1 | tRNA/rRNA methyltransferase SpoU family protei...    52   1e-06

>Medtr4g099300.1 | tRNA/rRNA methyltransferase SpoU family protein |
            HC | chr4:41173123-41201425 | 20130731
          Length = 1837

 Score =  666 bits (1718), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 323/403 (80%), Positives = 349/403 (86%), Gaps = 1/403 (0%)

Query: 2    QALSSYVFIAANVILHSSKDVQPRHLDELFPPLVPLLTSHHHSLRGFTQLLVYQILHKLF 61
            QALSSYVFIAANVIL+SSKDVQ  HLD+LFPPLVPLLTSHHHSLRGFTQLL+YQILHKLF
Sbjct: 1436 QALSSYVFIAANVILNSSKDVQSGHLDDLFPPLVPLLTSHHHSLRGFTQLLIYQILHKLF 1495

Query: 62   PLLNDGSSEILPLEKRCLVDLKLYLARNSDCARLRASMEGYLDAYNPNSSVTPAGIFVNR 121
            P+LN GSS+I PLEKRC VDLK YLARNSDCARLR SMEGYLDAYNPNSS TPAGIF+NR
Sbjct: 1496 PMLNYGSSDIPPLEKRCFVDLKTYLARNSDCARLRVSMEGYLDAYNPNSSATPAGIFINR 1555

Query: 122  VEEDNFECVPTSLMEQVLKFLNDVREDLRCSMAKDVVTLRNETLKSYGDTNCMKNVSGDT 181
             EE+NFECVPT LMEQVLKFLND REDLRCSMAKDVVT+RNETLK  GD +CM+ +SG +
Sbjct: 1556 AEENNFECVPTCLMEQVLKFLNDAREDLRCSMAKDVVTIRNETLKFNGD-DCMEKLSGGS 1614

Query: 182  EGIVLKDMSSDFQKKVTLAKHNKGDNEAGFLYSNDETYRKMAEIEXXXXXXXXXXXXXXX 241
            E  + KDMSSDFQKK+T  KH+ GDN+AG    +DETYRKMAEIE               
Sbjct: 1615 EAALFKDMSSDFQKKITFTKHDAGDNDAGLCCDDDETYRKMAEIERDDLLLDQVLQSRRS 1674

Query: 242  XXXXXXASRQNCILVASLLDRIPNLAGLARSCEVFGASGLAIADKNIISDKQFQLISVTA 301
                  ASRQ+ ILVASL+DRIPNLAGLAR+CEVF ASGLAIAD NII+DKQFQLISVTA
Sbjct: 1675 SLDQQKASRQSFILVASLIDRIPNLAGLARTCEVFKASGLAIADTNIINDKQFQLISVTA 1734

Query: 302  EKWVPIIELPVDSIKAYLQKKKREGYSILGLEQTANSVALDQYVFPQKMVLVLGREKEGI 361
            EKWVPIIE+PVDSIK YLQKKKREG+SILGLEQTANSV LDQY+FP+KMVLVLGREKEGI
Sbjct: 1735 EKWVPIIEVPVDSIKTYLQKKKREGFSILGLEQTANSVPLDQYIFPKKMVLVLGREKEGI 1794

Query: 362  PVDIIHILDACIEIPQLGVVRSLNVHVSGAIALWEYTRQQRSR 404
            PVDIIHILDAC+EIPQ GVVRSLNVHVSGAIALWEYTRQQR R
Sbjct: 1795 PVDIIHILDACVEIPQFGVVRSLNVHVSGAIALWEYTRQQRFR 1837


>Medtr7g012240.1 | tRNA/rRNA methyltransferase SpoU family protein |
           HC | chr7:3456747-3461056 | 20130731
          Length = 322

 Score = 52.0 bits (123), Expect = 1e-06,   Method: Compositional matrix adjust.
 Identities = 40/145 (27%), Positives = 70/145 (48%), Gaps = 8/145 (5%)

Query: 253 CILVASLLDRIPNLAGLARSCEVFGASGLAIADKNIISDKQFQ---LISVTAEKWVPIIE 309
           C++V  L D   N++   RS +  G   + +   +  ++K+++    +S+ AEKW+ I E
Sbjct: 130 CLVVEGLCD-FGNVSAAFRSADALGVQSVHVVSSD--ANKRYKDNRHVSMGAEKWLDI-E 185

Query: 310 LPVDSIKAYLQKKKREGYSILGLEQTANSVALDQYVFPQKMVLVLGREKEGIPVDIIHIL 369
           L  DS K   +  K  GY I       ++V++    +     +V+G E  GI  + + + 
Sbjct: 186 L-WDSTKECFEMLKSRGYRIATTHVGIDAVSIYDMDWSCPTAIVVGNENRGISDEALAMS 244

Query: 370 DACIEIPQLGVVRSLNVHVSGAIAL 394
           D    IP  G+V S NV V+  I +
Sbjct: 245 DLHCSIPMKGMVDSFNVSVAAGILM 269