Miyakogusa Predicted Gene
- Lj4g3v1073650.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj4g3v1073650.1 Non Characterized Hit- tr|G7I570|G7I570_MEDTR
Putative uncharacterized protein OS=Medicago
truncatul,72.19,0,DUF3537,Protein of unknown function DUF3537;
seg,NULL; SUBFAMILY NOT NAMED,NULL; FAMILY NOT NAMED,NU,CUFF.48551.1
(453 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr1g009230.1 | extracellular ligand-gated ion channel protein... 702 0.0
Medtr3g054010.1 | extracellular ligand-gated ion channel protein... 258 8e-69
Medtr2g025160.1 | extracellular ligand-gated ion channel protein... 236 4e-62
Medtr8g080210.1 | extracellular ligand-gated ion channel protein... 189 5e-48
Medtr2g025160.2 | extracellular ligand-gated ion channel protein... 187 2e-47
>Medtr1g009230.1 | extracellular ligand-gated ion channel protein |
HC | chr1:1241261-1238758 | 20130731
Length = 446
Score = 702 bits (1813), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 351/453 (77%), Positives = 387/453 (85%), Gaps = 13/453 (2%)
Query: 2 NNPSTNETSIDIGNSSGRRVSFAAENHPHPNGELTQHHQNNNVPLLLQPSYARSKSLVYD 61
+ ST +T+IDI NSS +RVSF+ + H QH NVPLLLQPSYARSKS+++D
Sbjct: 4 STESTKDTTIDIDNSSSKRVSFSDQQQQH------QH----NVPLLLQPSYARSKSMIFD 53
Query: 62 ELRNFRISLKWCALDHSSCVGKLISYFSFIFLTVIVPLFTSLFVEVPASAPEDDPFSFNK 121
ELRNFRI LKWCALDHSSCVGKLISY SFIFLT +VPLFT+LFV+V AS+PE DP S NK
Sbjct: 54 ELRNFRICLKWCALDHSSCVGKLISYVSFIFLTFVVPLFTTLFVQVSASSPEVDPISLNK 113
Query: 122 LVQIPESGLAIIAFFTLSCFFRRYGLRQLLFLDALEDDTTYVRRGYTRELEKAFKYLAYI 181
LVQIPES LAII+FFTL FFRRYGLRQLLFLDAL+DDTTYVRRGY RELEKAF+YLAYI
Sbjct: 114 LVQIPESALAIISFFTLYRFFRRYGLRQLLFLDALQDDTTYVRRGYARELEKAFRYLAYI 173
Query: 182 VLPSFFVELAHKITFFSAVKISTPYNTSPVLPLKVILFVLVLVSWVYRTGVFLLVCVFFR 241
+LPSFFVELAHKI FFSAVKIS P N S PL I+FV +L+SW+YRTGV+LLVCV F+
Sbjct: 174 ILPSFFVELAHKIIFFSAVKISAP-NLSAGFPLHSIVFVFMLLSWLYRTGVYLLVCVIFK 232
Query: 242 LTCQLQILRFEGVHKLFEGCGSDAGVIFREHVRIRRQLWVTSHRYRFFIVWCVVTMTVSQ 301
LTCQLQILRFEGVHKLFEGCGS+AGVIF+EHVRIRRQLW+ SHRYRFFI+ CVVTM+VSQ
Sbjct: 233 LTCQLQILRFEGVHKLFEGCGSEAGVIFKEHVRIRRQLWIISHRYRFFIICCVVTMSVSQ 292
Query: 302 LGALLLVLASKSDKTFFNSGDLVICSAVQLSGFFLCILGAAKITHRAQGIVSIATRWHML 361
LG+LLLVLASKS+KTFFNSGDLVICSAVQLSGFFLCILGAAKITHRAQGIVSIATRWHML
Sbjct: 293 LGSLLLVLASKSEKTFFNSGDLVICSAVQLSGFFLCILGAAKITHRAQGIVSIATRWHML 352
Query: 362 VTNASVESEQCKAEIPEGLAXXXXXXXXXIHISVV--PRLSSFQTRQSLVTYLQHNHGGI 419
VTNA ESEQCK + E LA + RLSSFQTRQSLVTYLQHNHGGI
Sbjct: 353 VTNAFAESEQCKDNMSEALASDSSDSDSSDIHISIIPQRLSSFQTRQSLVTYLQHNHGGI 412
Query: 420 TVYGFSLDRGLLHTLFAFEFSLVLWILSKVVVL 452
T+YGF+LDRG+LHTLFAFEFS+VLWILSKVVVL
Sbjct: 413 TLYGFALDRGMLHTLFAFEFSIVLWILSKVVVL 445
>Medtr3g054010.1 | extracellular ligand-gated ion channel protein |
HC | chr3:21416001-21420848 | 20130731
Length = 450
Score = 258 bits (659), Expect = 8e-69, Method: Compositional matrix adjust.
Identities = 162/467 (34%), Positives = 239/467 (51%), Gaps = 44/467 (9%)
Query: 7 NETSIDIGNSSGRRVSFAAENH-PHPNGELTQHHQNNNVPLLLQPSYARSKSLVYDELRN 65
NETS + ++++FA E+ P + +H N RS S V DEL
Sbjct: 2 NETS-----TPTKKINFAEESLLPKTTTNINRHKFN------------RSISHVNDELHT 44
Query: 66 FRISLKWCALDHSSCVGKLISYFSFIFLTVIVPLFTSLFVEV-PASAPEDDPFSFNKLVQ 124
FR L+W +D S+ + +S+F F + VP + F+ A P+ + +VQ
Sbjct: 45 FRSYLRWMCVDQSNAIHATLSWFVFFVFALAVPALSHFFLACEDCDARHSRPY--DSVVQ 102
Query: 125 IPESGLAIIAFFTLSCFFRRYGLRQLLFLDALEDDTTYVRRGYTRELEKAFKYLAYIVLP 184
I S +A+++F LS F ++YGLR+ LFLD L D++ VR Y +L ++ K ++ P
Sbjct: 103 ISLSSVAVLSFLCLSTFVKKYGLRRFLFLDKLCDESENVRMNYMVQLNRSLKLISVFSGP 162
Query: 185 SFFVELAHKITFFSAVKISTPYNTSPVLPLKVILFVLVLVSWVYRTGVFLLVCVFFRLTC 244
F +KI ++++ P+ + L + +L L SW+YRT LVCV FRL C
Sbjct: 163 CFIAMATYKIWWYASGGSQIPFLGNVYLS-DAVACILELCSWLYRTTTIFLVCVLFRLIC 221
Query: 245 QLQILRFEGVHKLFEGCGSDAGVIFREHVRIRRQLWVTSHRYRFFIVWCVVTMTVSQLGA 304
LQILR + LF SD + EH+RIRR L + SHRYR FI+ +V +T SQ
Sbjct: 222 HLQILRLKDFATLFH-VDSDVHSVMSEHLRIRRHLRIISHRYRAFILLALVLVTGSQFAC 280
Query: 305 LLLVLASKSDKTFFNSGDLVICSAVQLSGFFLCILGAAKITHRAQGIVSIATRWHMLVT- 363
LL+ + ++ D + +G+L +CS LS + A KITH+AQ I +A +WH+ T
Sbjct: 281 LLVTMKARHDLNVYKTGELALCSVTLLSALSIMFRSATKITHKAQAITGLAAKWHVCATL 340
Query: 364 ---NASVESEQCKAEIP----------EGLAXXXXXXXXXIHIS---VVPRLS----SFQ 403
+ E E A+I +G + I ++P S S+Q
Sbjct: 341 DSFDGVGEGETLSAQISRERIYPSVGTDGESETDDAGSEEDEIDTTKMIPSYSYSTISYQ 400
Query: 404 TRQSLVTYLQHNHGGITVYGFSLDRGLLHTLFAFEFSLVLWILSKVV 450
RQ+LV Y ++N GITVYGF LDR LHT+F E SLVLW+L K +
Sbjct: 401 KRQALVNYFENNKAGITVYGFMLDRSTLHTIFGIEMSLVLWLLGKTI 447
>Medtr2g025160.1 | extracellular ligand-gated ion channel protein |
HC | chr2:8949512-8952434 | 20130731
Length = 431
Score = 236 bits (602), Expect = 4e-62, Method: Compositional matrix adjust.
Identities = 141/409 (34%), Positives = 217/409 (53%), Gaps = 24/409 (5%)
Query: 62 ELRNFRISLKWCALDHSSCVGKLISYFSFIFLTVIVPLFTSLFVEVPASAPEDDPFSFNK 121
ELR FR L+W +D S+ IS+ F L +VP+ + ++ + D ++
Sbjct: 25 ELRRFRAYLRWVYVDQSNLCKAGISWSVFFTLAYVVPILSHFLLDCSTTCDADHRRPYHV 84
Query: 122 LVQIPESGLAIIAFFTLSCFFRRYGLRQLLFLDALEDDTTYVRRGYTRELEKAFKYLAYI 181
VQI S A ++F +LS + R+YG + LFLD + D++ ++RGY ++++ K +
Sbjct: 85 PVQISLSVFATLSFISLSKWDRKYGFSKFLFLDKVSDESLKIQRGYAIQMKRTMKLILLW 144
Query: 182 VLPSFFVELAHKITFFSAVKISTPYNTSPVLPLKVILFVLVLVSWVYRTGVFLLVCVFFR 241
LP F + +KI ++ + PY V +IL L L SW YRT +F LVCV FR
Sbjct: 145 GLPCFICQCVYKIWWYISGASQIPY-YGEVYVSGIILCTLELCSWFYRTSIFFLVCVLFR 203
Query: 242 LTCQLQILRFEGVHKLFEGCGSDAGVIFREHVRIRRQLWVTSHRYRFFIVWCVVTMTVSQ 301
L LQI R + +F+ ++ G I EH+RIRR L V SHR+R FI+ ++ +T SQ
Sbjct: 204 LIGYLQIQRLDEFAPVFQR-ETEVGTILLEHLRIRRNLRVISHRFRAFILSSLLLVTASQ 262
Query: 302 LGALLLVLASKSDKTFFNSGDLVICSAVQLSGFFLCILGAAKITHRAQGIVSIATRWHML 361
L LL+V+ +D G+L + S +SG ++ + A KITH+AQ + +A++WH+
Sbjct: 263 LIFLLMVIKPHADVDILKGGELGLVSITLVSGLYILLRSATKITHKAQSLTGLASKWHIC 322
Query: 362 VTNASVESEQCKAEIPEGLAXXXXXXXXXI-----------------HISVVP---RLSS 401
T S ++ E P L I + ++P R S
Sbjct: 323 ATINSFDN--IDGETPTALIASAQAMAADISWGSSEDESGDEEDELNNTKMMPIQTRTIS 380
Query: 402 FQTRQSLVTYLQHNHGGITVYGFSLDRGLLHTLFAFEFSLVLWILSKVV 450
F RQ+LVTY+++N GITVYGF LDR LH++F + +L LW+L+K V
Sbjct: 381 FHKRQALVTYMENNRAGITVYGFMLDRTWLHSIFGIQLALCLWLLNKTV 429
>Medtr8g080210.1 | extracellular ligand-gated ion channel protein |
HC | chr8:34461075-34457370 | 20130731
Length = 498
Score = 189 bits (480), Expect = 5e-48, Method: Compositional matrix adjust.
Identities = 123/413 (29%), Positives = 203/413 (49%), Gaps = 31/413 (7%)
Query: 55 SKSLVYDELRNFRISLKWCALDHSSCVGKLISYFSFIFLTVIVPLFTSLFVEVPASAPED 114
+K+ V L + L + S + +I + F L V VPL +L++ S E
Sbjct: 44 NKTEVDKSLARLELFLNILGFNPRSILSSIICWSGFFTLGVAVPL-VALWMG-DCSECEK 101
Query: 115 DPFSFNKLVQIP-ESGLAIIAFFTLSCFFRRYGLRQLLFLDALEDDTTYVRRGYTRELEK 173
S ++V + ++ LA ++ LS R+YGLR+ LF+D R Y ++
Sbjct: 102 YELSGCEMVIVAFQASLAAVSLLCLSHCLRKYGLRRFLFVDRYTGHIAAFHRDYVNQISG 161
Query: 174 AFKYLAYIVLPSFFVELAHKITFFSAVKISTPYNTSPVLPLKVILFVLVLVSWVYRTGVF 233
+ + V P F ++ +I I Y + +F+ ++VSW Y + +
Sbjct: 162 SLRLFILWVFPCFLLKTVREI-------IRISYIQHGSWGFSIAIFIALVVSWTYVSAIS 214
Query: 234 LLVCVFFRLTCQLQILRFEGVHKLFEGCGSDAGVIFREHVRIRRQLWVTSHRYRFFIVWC 293
L C+ F L C LQ++ F+ KL E SD V EH+R+R L SHR+R +++
Sbjct: 215 LTACIMFHLVCNLQVIHFDDYGKLLER-ESDVLVFLEEHMRLRYHLSKISHRFRVYLLLE 273
Query: 294 VVTMTVSQLGALLLVLASKSDKTFFNSGDLVICSAVQLSGFFLCILGAAKITHRAQGIVS 353
+ +TVSQ+ LL + + T N GD + + VQ+ G +C+ A +I+HRAQG+VS
Sbjct: 274 FLVVTVSQVVTLLQITGYRDMITMVNGGDFAVSTLVQVVGIIICLHAATRISHRAQGVVS 333
Query: 354 IATRWHMLVTNASVESEQCKAEIPEGLAXXXXXXXXXIHIS------------------- 394
+A+RWH + T AS ++ Q ++ G + IH+
Sbjct: 334 LASRWHAIATCASSDNSQMRSSASAG-SLEVANHLNSIHLDYSESDLESLDFGGMAVNTQ 392
Query: 395 VVPRLSSFQTRQSLVTYLQHNHGGITVYGFSLDRGLLHTLFAFEFSLVLWILS 447
++ +SS RQ+ V YLQ N GGIT++G+++DR L++T+F E SLV ++L
Sbjct: 393 LISYMSSHHKRQAFVMYLQSNPGGITIFGWTVDRSLVNTIFFLELSLVTFVLD 445
>Medtr2g025160.2 | extracellular ligand-gated ion channel protein |
HC | chr2:8949512-8952434 | 20130731
Length = 408
Score = 187 bits (474), Expect = 2e-47, Method: Compositional matrix adjust.
Identities = 110/319 (34%), Positives = 173/319 (54%), Gaps = 4/319 (1%)
Query: 62 ELRNFRISLKWCALDHSSCVGKLISYFSFIFLTVIVPLFTSLFVEVPASAPEDDPFSFNK 121
ELR FR L+W +D S+ IS+ F L +VP+ + ++ + D ++
Sbjct: 25 ELRRFRAYLRWVYVDQSNLCKAGISWSVFFTLAYVVPILSHFLLDCSTTCDADHRRPYHV 84
Query: 122 LVQIPESGLAIIAFFTLSCFFRRYGLRQLLFLDALEDDTTYVRRGYTRELEKAFKYLAYI 181
VQI S A ++F +LS + R+YG + LFLD + D++ ++RGY ++++ K +
Sbjct: 85 PVQISLSVFATLSFISLSKWDRKYGFSKFLFLDKVSDESLKIQRGYAIQMKRTMKLILLW 144
Query: 182 VLPSFFVELAHKITFFSAVKISTPYNTSPVLPLKVILFVLVLVSWVYRTGVFLLVCVFFR 241
LP F + +KI ++ + PY V +IL L L SW YRT +F LVCV FR
Sbjct: 145 GLPCFICQCVYKIWWYISGASQIPYY-GEVYVSGIILCTLELCSWFYRTSIFFLVCVLFR 203
Query: 242 LTCQLQILRFEGVHKLFEGCGSDAGVIFREHVRIRRQLWVTSHRYRFFIVWCVVTMTVSQ 301
L LQI R + +F+ ++ G I EH+RIRR L V SHR+R FI+ ++ +T SQ
Sbjct: 204 LIGYLQIQRLDEFAPVFQR-ETEVGTILLEHLRIRRNLRVISHRFRAFILSSLLLVTASQ 262
Query: 302 LGALLLVLASKSDKTFFNSGDLVICSAVQLSGFFLCILGAAKITHRAQGIVSIATRWHML 361
L LL+V+ +D G+L + S +SG ++ + A KITH+AQ + +A++WH+
Sbjct: 263 LIFLLMVIKPHADVDILKGGELGLVSITLVSGLYILLRSATKITHKAQSLTGLASKWHIC 322
Query: 362 VTNASVESEQCKAEIPEGL 380
T S ++ E P L
Sbjct: 323 ATINSFDN--IDGETPTAL 339