Miyakogusa Predicted Gene

Lj4g3v0877830.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v0877830.1 tr|I1L9S4|I1L9S4_SOYBN DNA topoisomerase 2
OS=Glycine max GN=Gma.48179 PE=3 SV=1,59.4,0,DNA_topoisoIV,DNA
topoisomerase, type IIA, subunit A/C-terminal; TOPISMRASEII,DNA
topoisomerase II, ,CUFF.48145.1
         (369 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr3g031040.1 | DNA topoisomerase IV subunit A | HC | chr3:987...   460   e-129
Medtr3g085840.1 | DNA topoisomerase IV subunit A | HC | chr3:388...   409   e-114
Medtr1g051315.1 | DNA topoisomerase IV | LC | chr1:20097532-2010...   349   2e-96
Medtr1g075370.1 | DNA topoisomerase IV | LC | chr1:33373055-3336...   339   2e-93
Medtr0756s0010.1 | DNA topoisomerase IV | LC | scaffold0756:6777...   253   2e-67
Medtr1g031690.1 | DNA gyrase subunit A | HC | chr1:11104096-1108...    63   4e-10
Medtr6g029520.1 | DNA topoisomerase | LC | chr6:10170084-1016718...    51   2e-06

>Medtr3g031040.1 | DNA topoisomerase IV subunit A | HC |
           chr3:9877949-9869222 | 20130731
          Length = 1504

 Score =  460 bits (1183), Expect = e-129,   Method: Compositional matrix adjust.
 Identities = 216/367 (58%), Positives = 278/367 (75%), Gaps = 3/367 (0%)

Query: 1   MESFIYGRVMFMADQDYDGSHIKGLLINFFYMLSPSLLRIPYFLSELITPIIRAFHCNGI 60
           ++S  YG +M MADQD+DGSHIKGLLINF +   PSLL++P F+ E  TPIIRA H NG 
Sbjct: 513 VKSLRYGHLMIMADQDHDGSHIKGLLINFIHSFWPSLLKVPSFMVEFTTPIIRASHKNGT 572

Query: 61  QKLSFYSYPEYEKWWKNWGNSD--WRIKYYKGLGSFTHEEGKEFFQNVEIQRKYFVWRND 118
            KLSFYS PEY+ W +  GN+   W IKYYKGLG+ T +EG+E+F++++  RK F+W N+
Sbjct: 573 -KLSFYSMPEYDAWRERLGNTANAWAIKYYKGLGTSTPQEGREYFKDLDKHRKDFIWENE 631

Query: 119 EDLDAINIAFSKSKTRERRGLMLKFKSSDYRDYTDLSICYKDFLQKEFILYSMASVRRCI 178
            D +AI +AFSK K  +R+  M  F+    RD+    I YKDF+ KE IL+S A ++R I
Sbjct: 632 LDGNAIELAFSKKKAEDRKNWMRSFEPGTCRDHEAKLISYKDFVDKELILFSRADLQRSI 691

Query: 179 PSLIDGQKPSQRKILYGAFKRELFTEIRVDKLIGYVAEHSAYHHDESSLATTLMRMARDY 238
           PS++DG KP QRKIL+ +FK+ L  E++V + IGYV+EHSAYHH E SLA+T++ MA+D+
Sbjct: 692 PSMVDGLKPGQRKILFCSFKKNLSKEVKVGQFIGYVSEHSAYHHGEQSLASTIIGMAQDF 751

Query: 239 VGSMNINLLMANGQFGTHIQGGKDHASPRYIYTELNPISRFLFPEIDNKLLKYLNEDGIS 298
           VGS NINLL  NGQFGT   GGKDHAS RYIYT+LN ++R LF   D+KLL+YLNEDG S
Sbjct: 752 VGSNNINLLTPNGQFGTRNLGGKDHASSRYIYTQLNTVTRSLFHADDDKLLEYLNEDGRS 811

Query: 299 VEPEWYLPIIPLVLVNGVSGIATGWNCSIPCYNPKDIIANIKRFLNDDKLQPMVPWYKGF 358
           +EP WY+PIIPLVLVNG  GI TGW+  IP YNP++IIAN++R LND+++ PM PWYKGF
Sbjct: 812 IEPNWYIPIIPLVLVNGSEGIGTGWSSFIPNYNPREIIANVRRLLNDEEMVPMDPWYKGF 871

Query: 359 KGTIDKS 365
           +GTI+KS
Sbjct: 872 RGTIEKS 878


>Medtr3g085840.1 | DNA topoisomerase IV subunit A | HC |
           chr3:38841641-38834996 | 20130731
          Length = 1345

 Score =  409 bits (1051), Expect = e-114,   Method: Compositional matrix adjust.
 Identities = 200/368 (54%), Positives = 258/368 (70%), Gaps = 32/368 (8%)

Query: 1   MESFIYGRVMFMADQDYDGSHIKGLLINFFYMLSPSLLRIPYFLSELITPIIRAFHCNGI 60
           ++S  YG +M MADQD+DGSHIKGLLINF +   PSLL++P F+ E  TPIIR  H NG 
Sbjct: 490 VKSLRYGHLMIMADQDHDGSHIKGLLINFIHSFWPSLLKVPSFIVEFTTPIIRVSHSNGT 549

Query: 61  QKLSFYSYPEYEKWWKNWGNSD--WRIKYYKGLGSFTHEEGKEFFQNVEIQRKYFVWRND 118
            KLSFYS  +YE W K  GN+   W+IKYYKGLG+ T +EG+E+FQ+++   K F+W   
Sbjct: 550 -KLSFYSMSDYEVWRKRIGNNANCWKIKYYKGLGTSTSQEGREYFQDLDKHMKEFIW--- 605

Query: 119 EDLDAINIAFSKSKTRERRGLMLKFKSSDYRDYTDLSICYKDFLQKEFILYSMASVRRCI 178
                                      S   D     I YK F+ +E IL+S A ++R I
Sbjct: 606 --------------------------PSTCHDLEAKLIRYKSFIDRELILFSRAELQRSI 639

Query: 179 PSLIDGQKPSQRKILYGAFKRELFTEIRVDKLIGYVAEHSAYHHDESSLATTLMRMARDY 238
           PS++DG KP QRKIL+ +FK++L+ EI+V + IGYV+ HSAYHH E SLA T++ MA+D+
Sbjct: 640 PSMVDGLKPGQRKILFCSFKKKLYKEIKVSQFIGYVSLHSAYHHGEQSLANTIIGMAQDF 699

Query: 239 VGSMNINLLMANGQFGTHIQGGKDHASPRYIYTELNPISRFLFPEIDNKLLKYLNEDGIS 298
           VG  NINLL  NGQFGT   GGKDHASP+YIYTELN ++RFLF E D+KLLKYLNE+G S
Sbjct: 700 VGGNNINLLNPNGQFGTRNLGGKDHASPKYIYTELNIVTRFLFHEDDDKLLKYLNEEGKS 759

Query: 299 VEPEWYLPIIPLVLVNGVSGIATGWNCSIPCYNPKDIIANIKRFLNDDKLQPMVPWYKGF 358
           +EP WY+PIIPLVLVNG   I TGW+  IP YNP++IIAN++R LN++++ PM PWY+GF
Sbjct: 760 IEPNWYIPIIPLVLVNGSEEIGTGWSSYIPNYNPREIIANVRRLLNEEEMVPMDPWYRGF 819

Query: 359 KGTIDKSI 366
           +GTI+KS+
Sbjct: 820 RGTIEKSM 827


>Medtr1g051315.1 | DNA topoisomerase IV | LC |
           chr1:20097532-20106196 | 20130731
          Length = 1178

 Score =  349 bits (896), Expect = 2e-96,   Method: Compositional matrix adjust.
 Identities = 182/376 (48%), Positives = 253/376 (67%), Gaps = 12/376 (3%)

Query: 2   ESFIYGRVMFMADQDYDGSHIKGLLINFFYMLSPSLLRIPYFLSELITPIIRAFHCNGIQ 61
           ES  YGR+M MA+QD DG+++KGLLINF Y   PSLL++  F+S    PII+A +    +
Sbjct: 433 ESLRYGRLMIMANQDEDGAYLKGLLINFIYSFWPSLLKVSKFMSVFTIPIIKASNSKEGK 492

Query: 62  KLSFYSYPEYEKWWKNWGNS---DWRIKYYKGLGSFTHEEGKEFFQNVEIQRKYFVWRND 118
           +LSF S  +YE W K+ GN+   DW I+Y KG  +   +E KE+FQ++  +R YFVW +D
Sbjct: 493 ELSFDSMQQYEDWEKDLGNTSTDDWEIEYCKGFTA--AQEVKEYFQDLGERRAYFVWDDD 550

Query: 119 EDLD----AINIAFSKSKTRERRGLML--KFKSSDYRDYTDLSICYKDFLQKEFILYSMA 172
           ++ D    +I +AFSK K  E +  +   +       +Y   SI Y+DF+ KE + +SM 
Sbjct: 551 DEQDGTSTSIELAFSK-KAEEWKAWVCNSQMMQPGTCNYESKSIKYRDFVNKELLFFSMK 609

Query: 173 SVRRCIPSLIDGQKPSQRKILYGAFKRELFTEIRVDKLIGYVAEHSAYHHDESSLATTLM 232
           +++R IPS++DG    QRK L+ +FK +L     V +L  YVA HS +H DE S+A+T++
Sbjct: 610 NLQRFIPSMVDGLTLGQRKTLFCSFKMKLTKPTEVFRLTTYVALHSFFHDDEQSIASTII 669

Query: 233 RMARDYVGSMNINLLMANGQFGTHIQGGKDHASPRYIYTELNPISRFLFPEIDNKLLKYL 292
            MA+D+VGS NINLL+  GQFGT   GGKDHAS R +  +LN ++R LFP  D+KLL+YL
Sbjct: 670 GMAQDFVGSNNINLLIPYGQFGTRELGGKDHASFRKLCNKLNCVTRLLFPVDDDKLLEYL 729

Query: 293 NEDGISVEPEWYLPIIPLVLVNGVSGIATGWNCSIPCYNPKDIIANIKRFLNDDKLQPMV 352
           NEDG SVEP WY+PIIPLVLVNG   + T ++  IP Y+P +II N++RFLN +++ PM 
Sbjct: 730 NEDGKSVEPNWYIPIIPLVLVNGCHAMGTCFSSDIPKYHPCEIIENVRRFLNKEEMVPMK 789

Query: 353 PWYKGFKGTIDKSIGG 368
           PWY+GF+GTI KS  G
Sbjct: 790 PWYRGFRGTIKKSAKG 805


>Medtr1g075370.1 | DNA topoisomerase IV | LC |
           chr1:33373055-33366326 | 20130731
          Length = 1000

 Score =  339 bits (870), Expect = 2e-93,   Method: Compositional matrix adjust.
 Identities = 176/366 (48%), Positives = 246/366 (67%), Gaps = 7/366 (1%)

Query: 6   YGRVMFMADQDYDGSHIKGLLINFFYMLSPSLLRIPYFLSELITPIIRAFHCNGIQKLSF 65
           YG++M MA+QD DG+H KGLLINFFY   PSLL++  F+S    PII+A      ++LSF
Sbjct: 373 YGQLMVMANQDEDGAHFKGLLINFFYSFWPSLLKVSKFMSVFTFPIIKASDSKKGKELSF 432

Query: 66  YSYPEYEKWWKNWGNS--DWRIKYYKGLGSFTHEEGKEFFQNVEIQRKYFVWRNDEDLDA 123
            S  +YE W K  GN+  DW IKY +GL S   +E +E+FQ++   R YFVW +D+D  +
Sbjct: 433 DSMQQYEDWKKELGNTANDWEIKYCQGLSS--AQEVREYFQDLGKHRAYFVW-DDKDETS 489

Query: 124 INIAFSKSKTRERRGLMLKFKSSDYRDYTDLSICYKDFLQKEFILYSMASVRRCIPSLID 183
           I +AFSK K  E    +   +     +Y    I Y++F+ KE +  SM +++R IPS++D
Sbjct: 490 IKLAFSK-KAEEWEAWIGNSQPGTC-NYERKVINYRNFVNKELLFSSMKNLQRSIPSVVD 547

Query: 184 GQKPSQRKILYGAFKRELFTEIRVDKLIGYVAEHSAYHHDESSLATTLMRMARDYVGSMN 243
           G    QRK L+ +F+  L  +  V +L  YV EHS  H+ + S+A+T++ MA+D+VGS N
Sbjct: 548 GLTLGQRKTLFCSFEMNLTKQTEVCRLTAYVTEHSYCHYGQQSIASTIIGMAQDFVGSNN 607

Query: 244 INLLMANGQFGTHIQGGKDHASPRYIYTELNPISRFLFPEIDNKLLKYLNEDGISVEPEW 303
           INLL+  GQFGT   GGKDHAS R +Y +LN ++R LFP  D+KLL+YLNEDG S++P W
Sbjct: 608 INLLIPYGQFGTRDLGGKDHASSRKLYIKLNCVTRLLFPVDDDKLLEYLNEDGRSIQPNW 667

Query: 304 YLPIIPLVLVNGVSGIATGWNCSIPCYNPKDIIANIKRFLNDDKLQPMVPWYKGFKGTID 363
           Y+PIIPLVLVNG   + T ++  IP Y+P +II N++RFLN++++  M PWYKGF GTI+
Sbjct: 668 YIPIIPLVLVNGCHAMGTCFSSDIPKYHPYEIIKNVRRFLNNEEMVSMKPWYKGFGGTIE 727

Query: 364 KSIGGM 369
           KS  G+
Sbjct: 728 KSAKGI 733


>Medtr0756s0010.1 | DNA topoisomerase IV | LC | scaffold0756:6777-31
           | 20130731
          Length = 996

 Score =  253 bits (646), Expect = 2e-67,   Method: Compositional matrix adjust.
 Identities = 142/311 (45%), Positives = 195/311 (62%), Gaps = 12/311 (3%)

Query: 2   ESFIYGRVMFMADQDYDGSHIKGLLINFFYMLSPSLLRIPYFLSELITPIIRAFHCNGIQ 61
           ES  YGR+M MA QD DG+ +KGLLINF Y   PSLL++  F+S    PI++A      +
Sbjct: 673 ESLRYGRLMIMAYQDEDGAQLKGLLINFIYSFWPSLLKVSKFMSVFTFPIMKASDLKKGK 732

Query: 62  KLSFYSYPEYEKWWKNWGNS--DWRIKYYKGLGSFTHEEGKEFFQNVEIQRKYFVWRNDE 119
           +LSF S  +YE W K  GN+  DW I Y KG  +    E +E+FQ++  +R YFVW +D+
Sbjct: 733 ELSFDSMQQYEDWIKELGNTANDWDINYCKGFSA--AREVREYFQDLGERRAYFVWDDDD 790

Query: 120 DLD----AINIAFSKSKTRERRGLMLKFKSSDYRDYTDLSICYKDFLQKEFILYSMASVR 175
           + D    +I +AFSK K  E    +   +     +Y   SI Y+DF+ KE + +SM +++
Sbjct: 791 EQDGTSTSIELAFSK-KAEEWMAWVCNSQPGTC-NYESKSIKYRDFVNKELLFFSMKNLQ 848

Query: 176 RCIPSLIDGQKPSQRKILYGAFKRELFTEIRVDKLIGYVAEHSAYHHD--ESSLATTLMR 233
             IPS++DG    QRK L+ +FK  L     V +L  YV +HS  H D  E S+A+T++ 
Sbjct: 849 GSIPSMVDGLTLGQRKTLFTSFKMNLTKPTEVFRLTAYVNQHSDCHGDGDEQSIASTIIG 908

Query: 234 MARDYVGSMNINLLMANGQFGTHIQGGKDHASPRYIYTELNPISRFLFPEIDNKLLKYLN 293
           MA+D+VGS NINLL+  GQFGT   GGKDHAS R + T LN ++R LFP  D+KLL+YLN
Sbjct: 909 MAQDFVGSNNINLLVPYGQFGTRDLGGKDHASFRKLCTNLNCVTRLLFPVDDDKLLEYLN 968

Query: 294 EDGISVEPEWY 304
           E+G S+EP WY
Sbjct: 969 ENGNSIEPNWY 979


>Medtr1g031690.1 | DNA gyrase subunit A | HC |
           chr1:11104096-11086066 | 20130731
          Length = 938

 Score = 63.2 bits (152), Expect = 4e-10,   Method: Compositional matrix adjust.
 Identities = 50/201 (24%), Positives = 103/201 (51%), Gaps = 11/201 (5%)

Query: 164 KEFILYSMASV-RRCIPSLIDGQKPSQRKILYGAFKRELFTEIRVDKLIGYVAE-HSAYH 221
           + ++ Y+M+ +  R +P + DG KP  R+IL+   +  L ++    K    V E    +H
Sbjct: 105 EAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELGLSSKKPFKKCARVVGEVLGKFH 164

Query: 222 -HDESSLATTLMRMARDYVGSMNINLLMANGQFGTHIQGGKDHASPRYIYTELNPISR-F 279
            H ++++  +++RMA+D+  S+   L+  +G FG+        A+ RY    L  ++   
Sbjct: 165 PHGDTAVYDSMVRMAQDF--SLRSPLVNGHGNFGSI--DADPPAAMRYTECRLEELAEAV 220

Query: 280 LFPEIDNKLLKYL-NEDGISVEPEWYLPIIPLVLVNGVSGIATGWNCSIPCYNPKDIIAN 338
           +  ++D   + ++ N D    EP      +P +L+NG SGIA G   +IP +N  +++  
Sbjct: 221 MLADLDQDTVDFVPNFDNSQKEPSLLPARLPTLLLNGSSGIAVGMATNIPPHNLGEVVDV 280

Query: 339 IKRFLNDDK--LQPMVPWYKG 357
           ++  +++ +  LQ ++ +  G
Sbjct: 281 LRVMIHNPEATLQELLEYMPG 301


>Medtr6g029520.1 | DNA topoisomerase | LC | chr6:10170084-10167186
          | 20130731
          Length = 100

 Score = 50.8 bits (120), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 31/85 (36%), Positives = 47/85 (55%), Gaps = 11/85 (12%)

Query: 6  YGRVMFMADQDYDGSHIKGLLINFFY-MLSPSLLRIPYFLSELITPIIRAFHCNGIQKLS 64
          YG+++ +A+ D+DG H+KGL++   Y M  P ++    FLS + T II+AF   G     
Sbjct: 17 YGKIIIIANADFDGIHMKGLILYLIYKMWIPIVMNRSKFLSTINTLIIKAFTTLG----- 71

Query: 65 FYSYPEYEKWWKNWGNSDWRIKYYK 89
                +E+  K    S W+IKYYK
Sbjct: 72 -----SFEELKKKVDLSGWKIKYYK 91