Miyakogusa Predicted Gene
- Lj4g3v0818970.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj4g3v0818970.1 Non Characterized Hit- tr|I1K265|I1K265_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.372
PE=4,83.21,0,UPF0034,tRNA-dihydrouridine synthase, conserved site;
FMN-linked oxidoreductases,NULL; no descriptio,CUFF.48089.1
(414 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr4g094905.1 | tRNA-dihydrouridine synthase-like protein | HC... 634 0.0
Medtr4g094902.1 | tRNA-dihydrouridine synthase-like protein | HC... 164 1e-40
Medtr8g067420.1 | tRNA-dihydrouridine synthase | HC | chr8:28151... 122 6e-28
Medtr8g067420.3 | tRNA-dihydrouridine synthase | HC | chr8:28151... 110 3e-24
Medtr8g067420.2 | tRNA-dihydrouridine synthase | HC | chr8:28151... 110 3e-24
Medtr3g117580.1 | tRNA-dihydrouridine synthase | HC | chr3:55052... 71 2e-12
Medtr4g037515.1 | tRNA-dihydrouridine synthase | HC | chr4:13808... 65 1e-10
Medtr3g030890.1 | tRNA-dihydrouridine synthase | HC | chr3:98123... 65 2e-10
>Medtr4g094905.1 | tRNA-dihydrouridine synthase-like protein | HC |
chr4:39249252-39247003 | 20130731
Length = 375
Score = 634 bits (1635), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 307/384 (79%), Positives = 332/384 (86%), Gaps = 21/384 (5%)
Query: 30 MAQTPPSPDPNSPQQTDNDDHQQPPFSPPRNLA--GETQVERAWAHWTKLGRPKFLVAPM 87
MAQT SPDP + PPRNL + +VERAWAHWTKLGRPK +VAPM
Sbjct: 1 MAQTRTSPDPENEH-------------PPRNLTLTADARVERAWAHWTKLGRPKLIVAPM 47
Query: 88 VDNSELPFRMLCRKYGAEGAYTPMLHSRIFTETEKYRKEEFTTCKEDRPLFVQFCANDPD 147
VDNSELP+RMLCRKYGA+ AYTPMLH RIFTE EKYR EEFTTCKEDRPLFVQFCANDPD
Sbjct: 48 VDNSELPYRMLCRKYGAQAAYTPMLHGRIFTEAEKYRNEEFTTCKEDRPLFVQFCANDPD 107
Query: 148 VLLEAARKVEPYCDYVDINLGCPQRIAKRGYYGAFLMDNLPLVKSLVENLALNLQVPVSC 207
+LLEAAR+VEP+ DYVDINLGCPQRIAK+G YGAFLMDNLPLVKSLVE LALNL+VPVSC
Sbjct: 108 ILLEAARRVEPFTDYVDINLGCPQRIAKKGNYGAFLMDNLPLVKSLVEKLALNLEVPVSC 167
Query: 208 KIRLFPNLEDTLKYARMLEEAGCFLLAVHGRTRDEKDGKKFRADWKAIKAVKDAVRIPVL 267
KIRLFP LEDTLKYA+MLEEAGC LLAVHGRTRDEKDG KFRADWKAIKAVK+AVRIPVL
Sbjct: 168 KIRLFPKLEDTLKYAKMLEEAGCLLLAVHGRTRDEKDGSKFRADWKAIKAVKEAVRIPVL 227
Query: 268 ANGNIRHVDDVRDCLEETGVEGVLSAETLLENPALFAGFRTAEWVSGSEGTIVDSEGTIV 327
ANGNIRH+DDV+DCLE TGVEGVLSAETLLENPALFAGFRT EWVSG E EGT V
Sbjct: 228 ANGNIRHMDDVKDCLEATGVEGVLSAETLLENPALFAGFRTEEWVSGCE------EGTFV 281
Query: 328 DGRLDQADLVIEYLKLCEKYPVPWRMIRSHVHKMLGDWFSLQPHIREELNQQSKLTFEFL 387
DG+LDQADL+IEYL LCEKYPVPWR+IRSHVHK+LGDWFSLQPHIRE+ N+Q K+TFE+L
Sbjct: 282 DGKLDQADLLIEYLNLCEKYPVPWRIIRSHVHKLLGDWFSLQPHIREDFNKQYKVTFEYL 341
Query: 388 YDMVDRLRATGTRIPLYLKDDRVE 411
YDMVD+LR TGTRIPLY K +E
Sbjct: 342 YDMVDQLRETGTRIPLYRKSTEME 365
>Medtr4g094902.1 | tRNA-dihydrouridine synthase-like protein | HC |
chr4:39244860-39244543 | 20130731
Length = 105
Score = 164 bits (415), Expect = 1e-40, Method: Composition-based stats.
Identities = 76/92 (82%), Positives = 84/92 (91%)
Query: 223 RMLEEAGCFLLAVHGRTRDEKDGKKFRADWKAIKAVKDAVRIPVLANGNIRHVDDVRDCL 282
+MLEEAGC LLAVHGR R++KDG KFRADWK IKAVK+AVRIPV ANGNIRH+DDV+DCL
Sbjct: 3 KMLEEAGCLLLAVHGRMRNKKDGHKFRADWKTIKAVKEAVRIPVHANGNIRHMDDVKDCL 62
Query: 283 EETGVEGVLSAETLLENPALFAGFRTAEWVSG 314
+ TGVEGVLSAETLLENPALFAGF T E+VSG
Sbjct: 63 KATGVEGVLSAETLLENPALFAGFCTEEYVSG 94
>Medtr8g067420.1 | tRNA-dihydrouridine synthase | HC |
chr8:28151579-28145915 | 20130731
Length = 303
Score = 122 bits (306), Expect = 6e-28, Method: Compositional matrix adjust.
Identities = 80/242 (33%), Positives = 120/242 (49%), Gaps = 18/242 (7%)
Query: 79 RPKFLVAPMVDNSELPFRMLCRKYGAEGAYTPMLHSRIFTETEKYRKEE----------- 127
R K ++APMV LP R+L +YGA+ Y + + E+ E
Sbjct: 4 RNKKILAPMVRVGTLPLRLLAAEYGADITYGEEIVDHKIIKCERRINEHLGSTDFVEKGT 63
Query: 128 ----FTTCKEDRPLFV-QFCANDPDVLLEAARKVEPYCDYVDINLGCPQRIAKRGYYGAF 182
F TC +++ V Q ++ L AA+ V VDIN+GCP+ + G GA
Sbjct: 64 ESVVFRTCDQEKNRVVFQIGTSNAVRALTAAQLVCNDVAAVDINMGCPKAFSLSGGMGAA 123
Query: 183 LMDNLPLVKSLVENLALNLQVPVSCKIRLFPNLEDTLKYARMLEEAGCFLLAVHGRTRDE 242
L+ L+ ++ L NL +PV+CKIRL + DT++ AR +E+ G +AVHGR
Sbjct: 124 LLSKPELISDILTTLRRNLSLPVTCKIRLLKSPHDTVELARRIEKTGVDAIAVHGRY--V 181
Query: 243 KDGKKFRADWKAIKAVKDAVRIPVLANGNIRHVDDVRDCLEETGVEGVLSAETLLENPAL 302
D + A W I V A+ IPV+ANG++ DD + TG V++A L NP++
Sbjct: 182 LDRPRDPAKWNGIADVVSALSIPVIANGDVFEYDDFQRIKSATGASSVMAARGALWNPSI 241
Query: 303 FA 304
F+
Sbjct: 242 FS 243
>Medtr8g067420.3 | tRNA-dihydrouridine synthase | HC |
chr8:28151611-28143518 | 20130731
Length = 253
Score = 110 bits (274), Expect = 3e-24, Method: Compositional matrix adjust.
Identities = 75/227 (33%), Positives = 111/227 (48%), Gaps = 18/227 (7%)
Query: 79 RPKFLVAPMVDNSELPFRMLCRKYGAEGAYTPMLHSRIFTETEKYRKEE----------- 127
R K ++APMV LP R+L +YGA+ Y + + E+ E
Sbjct: 4 RNKKILAPMVRVGTLPLRLLAAEYGADITYGEEIVDHKIIKCERRINEHLGSTDFVEKGT 63
Query: 128 ----FTTCKEDRPLFV-QFCANDPDVLLEAARKVEPYCDYVDINLGCPQRIAKRGYYGAF 182
F TC +++ V Q ++ L AA+ V VDIN+GCP+ + G GA
Sbjct: 64 ESVVFRTCDQEKNRVVFQIGTSNAVRALTAAQLVCNDVAAVDINMGCPKAFSLSGGMGAA 123
Query: 183 LMDNLPLVKSLVENLALNLQVPVSCKIRLFPNLEDTLKYARMLEEAGCFLLAVHGRTRDE 242
L+ L+ ++ L NL +PV+CKIRL + DT++ AR +E+ G +AVHGR
Sbjct: 124 LLSKPELISDILTTLRRNLSLPVTCKIRLLKSPHDTVELARRIEKTGVDAIAVHGRY--V 181
Query: 243 KDGKKFRADWKAIKAVKDAVRIPVLANGNIRHVDDVRDCLEETGVEG 289
D + A W I V A+ IPV+ANG++ DD + TG+ G
Sbjct: 182 LDRPRDPAKWNGIADVVSALSIPVIANGDVFEYDDFQRIKSATGMFG 228
>Medtr8g067420.2 | tRNA-dihydrouridine synthase | HC |
chr8:28151636-28143499 | 20130731
Length = 257
Score = 110 bits (274), Expect = 3e-24, Method: Compositional matrix adjust.
Identities = 75/227 (33%), Positives = 111/227 (48%), Gaps = 18/227 (7%)
Query: 79 RPKFLVAPMVDNSELPFRMLCRKYGAEGAYTPMLHSRIFTETEKYRKEE----------- 127
R K ++APMV LP R+L +YGA+ Y + + E+ E
Sbjct: 4 RNKKILAPMVRVGTLPLRLLAAEYGADITYGEEIVDHKIIKCERRINEHLGSTDFVEKGT 63
Query: 128 ----FTTCKEDRPLFV-QFCANDPDVLLEAARKVEPYCDYVDINLGCPQRIAKRGYYGAF 182
F TC +++ V Q ++ L AA+ V VDIN+GCP+ + G GA
Sbjct: 64 ESVVFRTCDQEKNRVVFQIGTSNAVRALTAAQLVCNDVAAVDINMGCPKAFSLSGGMGAA 123
Query: 183 LMDNLPLVKSLVENLALNLQVPVSCKIRLFPNLEDTLKYARMLEEAGCFLLAVHGRTRDE 242
L+ L+ ++ L NL +PV+CKIRL + DT++ AR +E+ G +AVHGR
Sbjct: 124 LLSKPELISDILTTLRRNLSLPVTCKIRLLKSPHDTVELARRIEKTGVDAIAVHGRY--V 181
Query: 243 KDGKKFRADWKAIKAVKDAVRIPVLANGNIRHVDDVRDCLEETGVEG 289
D + A W I V A+ IPV+ANG++ DD + TG+ G
Sbjct: 182 LDRPRDPAKWNGIADVVSALSIPVIANGDVFEYDDFQRIKSATGMFG 228
>Medtr3g117580.1 | tRNA-dihydrouridine synthase | HC |
chr3:55052867-55057843 | 20130731
Length = 691
Score = 71.2 bits (173), Expect = 2e-12, Method: Compositional matrix adjust.
Identities = 51/200 (25%), Positives = 84/200 (42%), Gaps = 8/200 (4%)
Query: 79 RPKFLVAPMVDNSELPFRMLCRKYGAEGAYTPMLHSRIFTETEKYRKEEFTTCKEDRPLF 138
R K +AP+ LPFR +C+ GA+ M E + +
Sbjct: 337 REKLYLAPLTTVGNLPFRRVCKVLGADITCGEMAMCTNLLEGQASEWALLRRHSSEDLFG 396
Query: 139 VQFCANDPDVLLEAARKVEPYC--DYVDINLGCPQRIAKRGYYGAFLMDNLPLVKSLVEN 196
VQ C PD + A +E C D++DIN+GCP I G+ L+ ++++VE
Sbjct: 397 VQICGAHPDTVGRAVELIEKECTVDFIDINMGCPIDIVVNKGAGSALLTKPMRMQNVVEV 456
Query: 197 LALNLQVPVSCKIRL--FPNLEDTLKYARMLEEAGCFLLAVHGRTRDEKDGKKFRADWKA 254
+ + +PV+ K+R F G + +HGR+R ++ K ADW
Sbjct: 457 ASGTVDIPVTIKVRTAYFEGRNRIDSLIANFSSWGASAVTIHGRSRQQRYSKP--ADWDY 514
Query: 255 IKAV--KDAVRIPVLANGNI 272
+ K + V+ NG++
Sbjct: 515 VYQCTRKAPNNLQVVGNGDV 534
>Medtr4g037515.1 | tRNA-dihydrouridine synthase | HC |
chr4:13808253-13802133 | 20130731
Length = 432
Score = 64.7 bits (156), Expect = 1e-10, Method: Compositional matrix adjust.
Identities = 62/242 (25%), Positives = 97/242 (40%), Gaps = 28/242 (11%)
Query: 80 PKFLVAPMVDNSELPFRMLCRKYGAEG-AYTPMLHSRIFTETEKYRKEEFTTCKEDRPLF 138
P F VAPM+D ++ +R L R YT ML + + E P+
Sbjct: 67 PWFSVAPMMDWTDNHYRTLARLISKHAWLYTEMLAAETIVHQKDNLDRFLAYSPEQHPIV 126
Query: 139 VQFCANDPDVLLEAARKVEPYC-DYVDINLGCPQ-RIAKRGYYGAFLMDNLPLVKSLVEN 196
+Q ++ + L +A YC D +++N GCP R+A G +G LM + V +
Sbjct: 127 LQIGGSNIESLAKATELANAYCYDEINLNCGCPSPRVAGHGSFGVSLMLDPKFVAEAMSA 186
Query: 197 LALNLQVPVSCKIRLFPNLEDTLKYARMLEEAGCFLLAVHG---------RTRDEKDGKK 247
+A N VPVS K R+ ++D Y + E F+ V +R
Sbjct: 187 IAANTNVPVSVKCRI--GVDDRDSYDELCE----FISTVSSLSPTKHFIIHSRKALLNGI 240
Query: 248 FRADWKAIKAVK---------DAVRIPVLANGNIRHVDDVRDCLEETGVEGVLSAETLLE 298
A+ ++I +K D + NG I VD+V + + G GV+
Sbjct: 241 SPAENRSIPPLKYEYFYGLLRDFPDLTFTINGGITSVDEV-NAARDAGAHGVMIGRAAFY 299
Query: 299 NP 300
NP
Sbjct: 300 NP 301
>Medtr3g030890.1 | tRNA-dihydrouridine synthase | HC |
chr3:9812366-9802443 | 20130731
Length = 375
Score = 64.7 bits (156), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 71/244 (29%), Positives = 107/244 (43%), Gaps = 32/244 (13%)
Query: 80 PKFLVAPMVDNSELPFRMLCRKYGAEG-AYTPMLHSRIFTETEKYRK---EEFTTCKEDR 135
P F VAPM+D ++ +R L R YT ML + ET Y+K + F D+
Sbjct: 9 PWFSVAPMMDWTDNHYRTLARLISKHAWLYTEMLAA----ETIVYQKGNLDRFLAYPPDQ 64
Query: 136 -PLFVQFCANDPDVLLEAARKVEPYC-DYVDINLGCPQ-RIAKRGYYGAFLMDNLPLVKS 192
P+ +Q ++ + L +A + + Y D ++ N GCP R+A RG +GA LM V
Sbjct: 65 HPIVLQIGGSNLENLAKATKLADAYGYDEINFNCGCPSPRVAGRGCFGARLMLEPKFVAE 124
Query: 193 LVENLALNLQVPVSCKIRL-------FPNLEDTLKYARMLEEAGCFLLAVHGRTRDEKDG 245
V +A N VPVS K R+ + L D + L A F+ +H R K
Sbjct: 125 AVSVIAANTNVPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPARHFI--IHSRKALLKGL 182
Query: 246 KKFRADWKAIKAVK---------DAVRIPVLANGNIRHVDDVRDCLEETGVEGVLSAETL 296
A+ ++I +K D + NG I +D++ L +TG GV+
Sbjct: 183 SP--AENRSIPPLKYEYFYGLLRDFPDLTFTINGGITCIDEINAAL-KTGAHGVMLGRAA 239
Query: 297 LENP 300
NP
Sbjct: 240 YHNP 243