Miyakogusa Predicted Gene

Lj4g3v0654580.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v0654580.1 Non Characterized Hit- tr|I3S077|I3S077_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,99.69,0,Glutathione S-transferase (GST), C-terminal
domain,Glutathione S-transferase, C-terminal-like; Thior,CUFF.48027.1
         (360 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g084040.1 | glutathione S-transferase-like protein | HC | ...   618   e-177
Medtr4g084040.2 | glutathione S-transferase-like protein | HC | ...   607   e-174
Medtr4g084040.3 | glutathione S-transferase-like protein | HC | ...   607   e-174
Medtr4g084040.4 | glutathione S-transferase-like protein | HC | ...   607   e-174
Medtr4g084040.5 | glutathione S-transferase-like protein | HC | ...   607   e-174
Medtr4g084040.6 | glutathione S-transferase-like protein | HC | ...   473   e-133
Medtr4g084040.7 | glutathione S-transferase-like protein | HC | ...   473   e-133
Medtr1g069575.1 | glutathione S-transferase-like protein | HC | ...   248   6e-66

>Medtr4g084040.1 | glutathione S-transferase-like protein | HC |
           chr4:32724728-32721844 | 20130731
          Length = 355

 Score =  618 bits (1594), Expect = e-177,   Method: Compositional matrix adjust.
 Identities = 287/330 (86%), Positives = 311/330 (94%)

Query: 31  KHIVEMVRSSLDEISDTGAFVRSASSFRQFVSRDPNSQFPAESGRYHLYISYACPWACRC 90
           KHIVEMVRSSLDEISD+GAF RSAS+FRQFVS+DPNSQFP ESGRYHLYISYACPWACRC
Sbjct: 26  KHIVEMVRSSLDEISDSGAFTRSASTFRQFVSKDPNSQFPPESGRYHLYISYACPWACRC 85

Query: 91  LAYLYIKGLDKAISVTSVKPIWGSITESGEFMGWVFPDTKTEVLGAEPDPLNGAKSIREI 150
           LAYL IKGLDKAIS +SVKPIWG   ES E+MGW+FP++ TEVLGAEPDPLNGAKS+RE+
Sbjct: 86  LAYLKIKGLDKAISFSSVKPIWGRTKESDEYMGWIFPESNTEVLGAEPDPLNGAKSVREL 145

Query: 151 YEIASTNYAGKYTVPILWDKKLKTIVNNESSEIIRMFNTEFNNIAENPTLDLYPADLRAQ 210
           YEIASTNY+GK+TVPILWDKKLKTIVNNESSEIIRMFNTEFN+IAENP LDLYP++L+AQ
Sbjct: 146 YEIASTNYSGKFTVPILWDKKLKTIVNNESSEIIRMFNTEFNDIAENPILDLYPSELQAQ 205

Query: 211 IDETNEWIYDSINNGVYKCGFAKKQEPYNDAARRLYEALDKCETILSKQRYLCGNTLTEA 270
           IDETNEWIY +INNGVY+CGFAKKQEPY DAAR+LYEALDKCE ILSKQRY+CGN LTEA
Sbjct: 206 IDETNEWIYPNINNGVYRCGFAKKQEPYIDAARQLYEALDKCEDILSKQRYICGNKLTEA 265

Query: 271 DIRLFVTLIRFDEVYAVHFKCNKKLLREYPNLFNYVKDIFQIPGVSNTVNMEHIKLHYYG 330
           DIRLFVTLIRFDEVYAVHFKCNKKL+REYPN+FNY KDIFQIPG+S+TVNMEHIKLHYYG
Sbjct: 266 DIRLFVTLIRFDEVYAVHFKCNKKLIREYPNIFNYTKDIFQIPGISSTVNMEHIKLHYYG 325

Query: 331 SHPSINPFAIVPAGPNIDYSAPHDRERFSA 360
           SHPSINPF IVP GPNIDY APHDRERFS 
Sbjct: 326 SHPSINPFGIVPMGPNIDYFAPHDRERFST 355


>Medtr4g084040.2 | glutathione S-transferase-like protein | HC |
           chr4:32724734-32721836 | 20130731
          Length = 325

 Score =  607 bits (1565), Expect = e-174,   Method: Compositional matrix adjust.
 Identities = 282/325 (86%), Positives = 306/325 (94%)

Query: 36  MVRSSLDEISDTGAFVRSASSFRQFVSRDPNSQFPAESGRYHLYISYACPWACRCLAYLY 95
           MVRSSLDEISD+GAF RSAS+FRQFVS+DPNSQFP ESGRYHLYISYACPWACRCLAYL 
Sbjct: 1   MVRSSLDEISDSGAFTRSASTFRQFVSKDPNSQFPPESGRYHLYISYACPWACRCLAYLK 60

Query: 96  IKGLDKAISVTSVKPIWGSITESGEFMGWVFPDTKTEVLGAEPDPLNGAKSIREIYEIAS 155
           IKGLDKAIS +SVKPIWG   ES E+MGW+FP++ TEVLGAEPDPLNGAKS+RE+YEIAS
Sbjct: 61  IKGLDKAISFSSVKPIWGRTKESDEYMGWIFPESNTEVLGAEPDPLNGAKSVRELYEIAS 120

Query: 156 TNYAGKYTVPILWDKKLKTIVNNESSEIIRMFNTEFNNIAENPTLDLYPADLRAQIDETN 215
           TNY+GK+TVPILWDKKLKTIVNNESSEIIRMFNTEFN+IAENP LDLYP++L+AQIDETN
Sbjct: 121 TNYSGKFTVPILWDKKLKTIVNNESSEIIRMFNTEFNDIAENPILDLYPSELQAQIDETN 180

Query: 216 EWIYDSINNGVYKCGFAKKQEPYNDAARRLYEALDKCETILSKQRYLCGNTLTEADIRLF 275
           EWIY +INNGVY+CGFAKKQEPY DAAR+LYEALDKCE ILSKQRY+CGN LTEADIRLF
Sbjct: 181 EWIYPNINNGVYRCGFAKKQEPYIDAARQLYEALDKCEDILSKQRYICGNKLTEADIRLF 240

Query: 276 VTLIRFDEVYAVHFKCNKKLLREYPNLFNYVKDIFQIPGVSNTVNMEHIKLHYYGSHPSI 335
           VTLIRFDEVYAVHFKCNKKL+REYPN+FNY KDIFQIPG+S+TVNMEHIKLHYYGSHPSI
Sbjct: 241 VTLIRFDEVYAVHFKCNKKLIREYPNIFNYTKDIFQIPGISSTVNMEHIKLHYYGSHPSI 300

Query: 336 NPFAIVPAGPNIDYSAPHDRERFSA 360
           NPF IVP GPNIDY APHDRERFS 
Sbjct: 301 NPFGIVPMGPNIDYFAPHDRERFST 325


>Medtr4g084040.3 | glutathione S-transferase-like protein | HC |
           chr4:32724737-32721836 | 20130731
          Length = 325

 Score =  607 bits (1565), Expect = e-174,   Method: Compositional matrix adjust.
 Identities = 282/325 (86%), Positives = 306/325 (94%)

Query: 36  MVRSSLDEISDTGAFVRSASSFRQFVSRDPNSQFPAESGRYHLYISYACPWACRCLAYLY 95
           MVRSSLDEISD+GAF RSAS+FRQFVS+DPNSQFP ESGRYHLYISYACPWACRCLAYL 
Sbjct: 1   MVRSSLDEISDSGAFTRSASTFRQFVSKDPNSQFPPESGRYHLYISYACPWACRCLAYLK 60

Query: 96  IKGLDKAISVTSVKPIWGSITESGEFMGWVFPDTKTEVLGAEPDPLNGAKSIREIYEIAS 155
           IKGLDKAIS +SVKPIWG   ES E+MGW+FP++ TEVLGAEPDPLNGAKS+RE+YEIAS
Sbjct: 61  IKGLDKAISFSSVKPIWGRTKESDEYMGWIFPESNTEVLGAEPDPLNGAKSVRELYEIAS 120

Query: 156 TNYAGKYTVPILWDKKLKTIVNNESSEIIRMFNTEFNNIAENPTLDLYPADLRAQIDETN 215
           TNY+GK+TVPILWDKKLKTIVNNESSEIIRMFNTEFN+IAENP LDLYP++L+AQIDETN
Sbjct: 121 TNYSGKFTVPILWDKKLKTIVNNESSEIIRMFNTEFNDIAENPILDLYPSELQAQIDETN 180

Query: 216 EWIYDSINNGVYKCGFAKKQEPYNDAARRLYEALDKCETILSKQRYLCGNTLTEADIRLF 275
           EWIY +INNGVY+CGFAKKQEPY DAAR+LYEALDKCE ILSKQRY+CGN LTEADIRLF
Sbjct: 181 EWIYPNINNGVYRCGFAKKQEPYIDAARQLYEALDKCEDILSKQRYICGNKLTEADIRLF 240

Query: 276 VTLIRFDEVYAVHFKCNKKLLREYPNLFNYVKDIFQIPGVSNTVNMEHIKLHYYGSHPSI 335
           VTLIRFDEVYAVHFKCNKKL+REYPN+FNY KDIFQIPG+S+TVNMEHIKLHYYGSHPSI
Sbjct: 241 VTLIRFDEVYAVHFKCNKKLIREYPNIFNYTKDIFQIPGISSTVNMEHIKLHYYGSHPSI 300

Query: 336 NPFAIVPAGPNIDYSAPHDRERFSA 360
           NPF IVP GPNIDY APHDRERFS 
Sbjct: 301 NPFGIVPMGPNIDYFAPHDRERFST 325


>Medtr4g084040.4 | glutathione S-transferase-like protein | HC |
           chr4:32724728-32721836 | 20130731
          Length = 325

 Score =  607 bits (1565), Expect = e-174,   Method: Compositional matrix adjust.
 Identities = 282/325 (86%), Positives = 306/325 (94%)

Query: 36  MVRSSLDEISDTGAFVRSASSFRQFVSRDPNSQFPAESGRYHLYISYACPWACRCLAYLY 95
           MVRSSLDEISD+GAF RSAS+FRQFVS+DPNSQFP ESGRYHLYISYACPWACRCLAYL 
Sbjct: 1   MVRSSLDEISDSGAFTRSASTFRQFVSKDPNSQFPPESGRYHLYISYACPWACRCLAYLK 60

Query: 96  IKGLDKAISVTSVKPIWGSITESGEFMGWVFPDTKTEVLGAEPDPLNGAKSIREIYEIAS 155
           IKGLDKAIS +SVKPIWG   ES E+MGW+FP++ TEVLGAEPDPLNGAKS+RE+YEIAS
Sbjct: 61  IKGLDKAISFSSVKPIWGRTKESDEYMGWIFPESNTEVLGAEPDPLNGAKSVRELYEIAS 120

Query: 156 TNYAGKYTVPILWDKKLKTIVNNESSEIIRMFNTEFNNIAENPTLDLYPADLRAQIDETN 215
           TNY+GK+TVPILWDKKLKTIVNNESSEIIRMFNTEFN+IAENP LDLYP++L+AQIDETN
Sbjct: 121 TNYSGKFTVPILWDKKLKTIVNNESSEIIRMFNTEFNDIAENPILDLYPSELQAQIDETN 180

Query: 216 EWIYDSINNGVYKCGFAKKQEPYNDAARRLYEALDKCETILSKQRYLCGNTLTEADIRLF 275
           EWIY +INNGVY+CGFAKKQEPY DAAR+LYEALDKCE ILSKQRY+CGN LTEADIRLF
Sbjct: 181 EWIYPNINNGVYRCGFAKKQEPYIDAARQLYEALDKCEDILSKQRYICGNKLTEADIRLF 240

Query: 276 VTLIRFDEVYAVHFKCNKKLLREYPNLFNYVKDIFQIPGVSNTVNMEHIKLHYYGSHPSI 335
           VTLIRFDEVYAVHFKCNKKL+REYPN+FNY KDIFQIPG+S+TVNMEHIKLHYYGSHPSI
Sbjct: 241 VTLIRFDEVYAVHFKCNKKLIREYPNIFNYTKDIFQIPGISSTVNMEHIKLHYYGSHPSI 300

Query: 336 NPFAIVPAGPNIDYSAPHDRERFSA 360
           NPF IVP GPNIDY APHDRERFS 
Sbjct: 301 NPFGIVPMGPNIDYFAPHDRERFST 325


>Medtr4g084040.5 | glutathione S-transferase-like protein | HC |
           chr4:32724734-32721836 | 20130731
          Length = 325

 Score =  607 bits (1565), Expect = e-174,   Method: Compositional matrix adjust.
 Identities = 282/325 (86%), Positives = 306/325 (94%)

Query: 36  MVRSSLDEISDTGAFVRSASSFRQFVSRDPNSQFPAESGRYHLYISYACPWACRCLAYLY 95
           MVRSSLDEISD+GAF RSAS+FRQFVS+DPNSQFP ESGRYHLYISYACPWACRCLAYL 
Sbjct: 1   MVRSSLDEISDSGAFTRSASTFRQFVSKDPNSQFPPESGRYHLYISYACPWACRCLAYLK 60

Query: 96  IKGLDKAISVTSVKPIWGSITESGEFMGWVFPDTKTEVLGAEPDPLNGAKSIREIYEIAS 155
           IKGLDKAIS +SVKPIWG   ES E+MGW+FP++ TEVLGAEPDPLNGAKS+RE+YEIAS
Sbjct: 61  IKGLDKAISFSSVKPIWGRTKESDEYMGWIFPESNTEVLGAEPDPLNGAKSVRELYEIAS 120

Query: 156 TNYAGKYTVPILWDKKLKTIVNNESSEIIRMFNTEFNNIAENPTLDLYPADLRAQIDETN 215
           TNY+GK+TVPILWDKKLKTIVNNESSEIIRMFNTEFN+IAENP LDLYP++L+AQIDETN
Sbjct: 121 TNYSGKFTVPILWDKKLKTIVNNESSEIIRMFNTEFNDIAENPILDLYPSELQAQIDETN 180

Query: 216 EWIYDSINNGVYKCGFAKKQEPYNDAARRLYEALDKCETILSKQRYLCGNTLTEADIRLF 275
           EWIY +INNGVY+CGFAKKQEPY DAAR+LYEALDKCE ILSKQRY+CGN LTEADIRLF
Sbjct: 181 EWIYPNINNGVYRCGFAKKQEPYIDAARQLYEALDKCEDILSKQRYICGNKLTEADIRLF 240

Query: 276 VTLIRFDEVYAVHFKCNKKLLREYPNLFNYVKDIFQIPGVSNTVNMEHIKLHYYGSHPSI 335
           VTLIRFDEVYAVHFKCNKKL+REYPN+FNY KDIFQIPG+S+TVNMEHIKLHYYGSHPSI
Sbjct: 241 VTLIRFDEVYAVHFKCNKKLIREYPNIFNYTKDIFQIPGISSTVNMEHIKLHYYGSHPSI 300

Query: 336 NPFAIVPAGPNIDYSAPHDRERFSA 360
           NPF IVP GPNIDY APHDRERFS 
Sbjct: 301 NPFGIVPMGPNIDYFAPHDRERFST 325


>Medtr4g084040.6 | glutathione S-transferase-like protein | HC |
           chr4:32724734-32721836 | 20130731
          Length = 259

 Score =  473 bits (1217), Expect = e-133,   Method: Compositional matrix adjust.
 Identities = 219/254 (86%), Positives = 239/254 (94%)

Query: 107 SVKPIWGSITESGEFMGWVFPDTKTEVLGAEPDPLNGAKSIREIYEIASTNYAGKYTVPI 166
           SVKPIWG   ES E+MGW+FP++ TEVLGAEPDPLNGAKS+RE+YEIASTNY+GK+TVPI
Sbjct: 6   SVKPIWGRTKESDEYMGWIFPESNTEVLGAEPDPLNGAKSVRELYEIASTNYSGKFTVPI 65

Query: 167 LWDKKLKTIVNNESSEIIRMFNTEFNNIAENPTLDLYPADLRAQIDETNEWIYDSINNGV 226
           LWDKKLKTIVNNESSEIIRMFNTEFN+IAENP LDLYP++L+AQIDETNEWIY +INNGV
Sbjct: 66  LWDKKLKTIVNNESSEIIRMFNTEFNDIAENPILDLYPSELQAQIDETNEWIYPNINNGV 125

Query: 227 YKCGFAKKQEPYNDAARRLYEALDKCETILSKQRYLCGNTLTEADIRLFVTLIRFDEVYA 286
           Y+CGFAKKQEPY DAAR+LYEALDKCE ILSKQRY+CGN LTEADIRLFVTLIRFDEVYA
Sbjct: 126 YRCGFAKKQEPYIDAARQLYEALDKCEDILSKQRYICGNKLTEADIRLFVTLIRFDEVYA 185

Query: 287 VHFKCNKKLLREYPNLFNYVKDIFQIPGVSNTVNMEHIKLHYYGSHPSINPFAIVPAGPN 346
           VHFKCNKKL+REYPN+FNY KDIFQIPG+S+TVNMEHIKLHYYGSHPSINPF IVP GPN
Sbjct: 186 VHFKCNKKLIREYPNIFNYTKDIFQIPGISSTVNMEHIKLHYYGSHPSINPFGIVPMGPN 245

Query: 347 IDYSAPHDRERFSA 360
           IDY APHDRERFS 
Sbjct: 246 IDYFAPHDRERFST 259


>Medtr4g084040.7 | glutathione S-transferase-like protein | HC |
           chr4:32724734-32721836 | 20130731
          Length = 259

 Score =  473 bits (1217), Expect = e-133,   Method: Compositional matrix adjust.
 Identities = 219/254 (86%), Positives = 239/254 (94%)

Query: 107 SVKPIWGSITESGEFMGWVFPDTKTEVLGAEPDPLNGAKSIREIYEIASTNYAGKYTVPI 166
           SVKPIWG   ES E+MGW+FP++ TEVLGAEPDPLNGAKS+RE+YEIASTNY+GK+TVPI
Sbjct: 6   SVKPIWGRTKESDEYMGWIFPESNTEVLGAEPDPLNGAKSVRELYEIASTNYSGKFTVPI 65

Query: 167 LWDKKLKTIVNNESSEIIRMFNTEFNNIAENPTLDLYPADLRAQIDETNEWIYDSINNGV 226
           LWDKKLKTIVNNESSEIIRMFNTEFN+IAENP LDLYP++L+AQIDETNEWIY +INNGV
Sbjct: 66  LWDKKLKTIVNNESSEIIRMFNTEFNDIAENPILDLYPSELQAQIDETNEWIYPNINNGV 125

Query: 227 YKCGFAKKQEPYNDAARRLYEALDKCETILSKQRYLCGNTLTEADIRLFVTLIRFDEVYA 286
           Y+CGFAKKQEPY DAAR+LYEALDKCE ILSKQRY+CGN LTEADIRLFVTLIRFDEVYA
Sbjct: 126 YRCGFAKKQEPYIDAARQLYEALDKCEDILSKQRYICGNKLTEADIRLFVTLIRFDEVYA 185

Query: 287 VHFKCNKKLLREYPNLFNYVKDIFQIPGVSNTVNMEHIKLHYYGSHPSINPFAIVPAGPN 346
           VHFKCNKKL+REYPN+FNY KDIFQIPG+S+TVNMEHIKLHYYGSHPSINPF IVP GPN
Sbjct: 186 VHFKCNKKLIREYPNIFNYTKDIFQIPGISSTVNMEHIKLHYYGSHPSINPFGIVPMGPN 245

Query: 347 IDYSAPHDRERFSA 360
           IDY APHDRERFS 
Sbjct: 246 IDYFAPHDRERFST 259


>Medtr1g069575.1 | glutathione S-transferase-like protein | HC |
           chr1:30231346-30229576 | 20130731
          Length = 407

 Score =  248 bits (634), Expect = 6e-66,   Method: Compositional matrix adjust.
 Identities = 139/332 (41%), Positives = 188/332 (56%), Gaps = 23/332 (6%)

Query: 35  EMVRSSLDEISDTGAFVRSASSFRQFVSRDPNSQFPAESGRYHLYISYACPWACRCLAYL 94
           +++ S L     TG + R AS FR F    PN          HLY+   CPWA R L   
Sbjct: 85  QLMMSQLAPSDPTGGYSRPASKFR-FSGPPPNG--------LHLYVGLPCPWAHRTLIVR 135

Query: 95  YIKGLDKAISVTSVKPIWGSITESGEFMGWVFP--DTKTEVLGAEPDPLNGAKSIREIYE 152
            +KGL+ A+ ++   P        G    W F       +V+    D  NG K+++E+Y 
Sbjct: 136 ALKGLEDAVPLSVASP--------GMDGSWEFKRVGNTGQVISPSLDKANGCKTLKEVYR 187

Query: 153 IASTNYAGKYTVPILWDKKLKTIVNNESSEIIRMFNTEFNNIAENPTLDLYPADLRAQID 212
           + S  Y G+ TVP+LW+K  K +V NES +II++FN+  N +A NP LDL P +L+ +I+
Sbjct: 188 LRSGGYDGRSTVPMLWNKDSKDVVCNESYDIIQLFNSGLNGLAGNPDLDLSPPELKGKIE 247

Query: 213 ETNEWIYDSINNGVYKCGFAKKQEPYNDAARRLYEALDKCETILSKQRYLCGNTLTEADI 272
           E  + IY ++NNGVY+CGFA+ QE Y+ A   L+  LDK E  LS  RYLCG+TLT  DI
Sbjct: 248 EWYQVIYPNVNNGVYRCGFAQSQEAYDRAVNELFCTLDKLEDHLSNSRYLCGDTLTLVDI 307

Query: 273 RLFVTLIRFDEVYAVHFKCNKKLLREYPNLFNYVKDIFQIPGVSNTVNMEHIKLHYYGSH 332
            LF TLIRFD  Y V FKC KK L EY NL  Y++DI+QIP V++T N + I   YY + 
Sbjct: 308 CLFTTLIRFDIAYNVLFKCTKKKLYEYTNLHAYMRDIYQIPKVADTCNFQEIMDGYYITL 367

Query: 333 PSINPFAIVPAGPNID----YSAPHDRERFSA 360
             +NP +I P  P+         PH RE  S+
Sbjct: 368 FPLNPGSIRPIMPSTSEHEILCRPHGRESLSS 399